Results for “Community Science” — Page 90

NASA NTRSDate not supplied

The Tables of Aerosol Optics (TAO) Project

The Table of Aerosol Optics (TAO) project is a community repository of optics computations (extinction, absorption, single-scatter albedo, lidar ratio, etc) that are useful for global models and remote sensing applications. TAO expands upon historical efforts (e.g., Hess et al., 1998) by building an open database that uses recent measurements and new computational techniques for non-spherical particles. The ‘open’ aspect of TAO is important, since the size distributions, hygroscopicities, refractive indices, and morphological recommendations of today will undoubtedly yield to different values in the future; the open framework of TAO allows scientists to keep adding new computations to the database as the science evolves. TAO is meant to be a community repository where specialists can put their computations for other scientists to use. So for instance, some groups are advancing new techniques that can accommodate complex fractal aggregates of black carbon, other groups are working on realistic irregular shapes for mineral dust, and different groups are updating the hygroscopicity of various aerosol types using new techniques. The TAO database gives these scientists a place to distribute their products. As TAO grows, modelers and remote sensing specialists will look to TAO as a place to find a wide variety of choices for testing. Meanwhile, global modelers can also use TAO to lobby for new tables that accommodate their needs. Eventually, TAO will provide mass extinction coefficients, mass absorption coefficients, lidar ratios, etc., at popular remote sensing and global modeling wavelengths (0.25-40 µm) for all pertinent species (sulfate, sea salt, BC, OC, BrC, dust, etc.). TAO will also accept computations for aerosol ‘type’ (e.g., biomass burning, urban, background, etc.) that may include regional and seasonal variability. Multiple tables may be created for each species or type to account for the multiple valid size distributions, hygroscopicities, complex refractive indices, and shapes that can be found in the literature.

Greg Schuster↗

NASA NTRSDate not supplied

The Tables of Aerosol Optics (TAO) Project

The Table of Aerosol Optics (TAO) project is a community repository of optics computations (extinction, absorption, single-scatter albedo, lidar ratio, etc) that are useful for global models and remote sensing applications. TAO expands upon historical efforts (e.g., Hess et al., 1998) by building an open database that uses recent measurements and new computational techniques for non-spherical particles. The ‘open’ aspect of TAO is important, since the size distributions, hygroscopicities, refractive indices, and morphological recommendations of today will undoubtedly yield to different values in the future; the open framework of TAO allows scientists to keep adding new computations to the database as the science evolves. TAO is meant to be a community repository where specialists can put their computations for other scientists to use. So for instance, some groups are advancing new techniques that can accommodate complex fractal aggregates of black carbon, other groups are working on realistic irregular shapes for mineral dust, and different groups are updating the hygroscopicity of various aerosol types using new techniques. The TAO database gives these scientists a place to distribute their products. As TAO grows, modelers and remote sensing specialists will look to TAO as a place to find a wide variety of choices for testing. Meanwhile, global modelers can also use TAO to lobby for new tables that accommodate their needs. Eventually, TAO will provide mass extinction coefficients, mass absorption coefficients, lidar ratios, etc., at popular remote sensing and global modeling wavelengths (0.25-40 µm) for all pertinent species (sulfate, sea salt, BC, OC, BrC, dust, etc.). TAO will also accept computations for aerosol ‘type’ (e.g., biomass burning, urban, background, etc.) that may include regional and seasonal variability. Multiple tables may be created for each species or type to account for the multiple valid size distributions, hygroscopicities, complex refractive indices, and shapes that can be found in the literature.

Elisabeth Andrews↗

NASA NTRSDate not supplied

The Tables of Aerosol Optics (TAO) Project

The Table of Aerosol Optics (TAO) project is a community repository of optics computations (extinction, absorption, single-scatter albedo, lidar ratio, etc) that are useful for global models and remote sensing applications. TAO expands upon historical efforts (e.g., Hess et al., 1998) by building an open database that uses recent measurements and new computational techniques for non-spherical particles. The ‘open’ aspect of TAO is important, since the size distributions, hygroscopicities, refractive indices, and morphological recommendations of today will undoubtedly yield to different values in the future; the open framework of TAO allows scientists to keep adding new computations to the database as the science evolves. TAO is meant to be a community repository where specialists can put their computations for other scientists to use. So for instance, some groups are advancing new techniques that can accommodate complex fractal aggregates of black carbon, other groups are working on realistic irregular shapes for mineral dust, and different groups are updating the hygroscopicity of various aerosol types using new techniques. The TAO database gives these scientists a place to distribute their products. As TAO grows, modelers and remote sensing specialists will look to TAO as a place to find a wide variety of choices for testing. Meanwhile, global modelers can also use TAO to lobby for new tables that accommodate their needs. Eventually, TAO will provide mass extinction coefficients, mass absorption coefficients, lidar ratios, etc., at popular remote sensing and global modeling wavelengths (0.25-40 µm) for all pertinent species (sulfate, sea salt, BC, OC, BrC, dust, etc.). TAO will also accept computations for aerosol ‘type’ (e.g., biomass burning, urban, background, etc.) that may include regional and seasonal variability. Multiple tables may be created for each species or type to account for the multiple valid size distributions, hygroscopicities, complex refractive indices, and shapes that can be found in the literature.

Greg Schuster↗

DOE OSTI2020

The Ontologies Community of Practice: A CGIAR Initiative for Big Data in Agrifood Systems

Heterogeneous and multidisciplinary data generated by research on sustainable global agriculture and agrifood systems requires quality data labeling or annotation in order to be interoperable. As recommended by the FAIR principles, data, labels, and metadata must use controlled vocabularies and ontologies that are popular in the knowledge domain and commonly used by the community. Despite the existence of robust ontologies in the Life Sciences, there is currently no comprehensive full set of ontologies recommended for data annotation across agricultural research disciplines. In this paper, we discuss the added value of the Ontologies Community of Practice (CoP) of the CGIAR Platform for Big Data in Agriculture for harnessing relevant expertise in ontology development and identifying innovative solutions that support quality data annotation. The Ontologies CoP stimulates knowledge sharing among stakeholders, such as researchers, data managers, domain experts, experts in ontology design, and platform development teams.

96 KNOWLEDGE MANAGEMENT AND PRESERVATION↗

NASA NTRS2011

Improvements in Space Geodesy Data Discovery at the CDDIS

The Crustal Dynamics Data Information System (CDDIS) supports data archiving and distribution activities for the space geodesy and geodynamics community. The main objectives of the system are to store space geodesy and geodynamics related data products in a central data bank. to maintain information about the archival of these data, and to disseminate these data and information in a timely manner to a global scientific research community. The archive consists of GNSS, laser ranging, VLBI, and DORIS data sets and products derived from these data. The CDDIS is one of NASA's Earth Observing System Data and Information System (EOSDIS) distributed data centers; EOSDIS data centers serve a diverse user community and arc tasked to provide facilities to search and access science data and products. Several activities are currently under development at the CDDIS to aid users in data discovery, both within the current community and beyond. The CDDIS is cooperating in the development of Geodetic Seamless Archive Centers (GSAC) with colleagues at UNAVCO and SIO. TIle activity will provide web services to facilitate data discovery within and across participating archives. In addition, the CDDIS is currently implementing modifications to the metadata extracted from incoming data and product files pushed to its archive. These enhancements will permit information about COOlS archive holdings to be made available through other data portals such as Earth Observing System (EOS) Clearinghouse (ECHO) and integration into the Global Geodetic Observing System (GGOS) portal.

Noll, C.↗

NASA NTRSDate not supplied

Identifying Improvements to Airborne and Field Data Stewardship from Data User and Producer Needs (Findings from the March 2022 Airborne and Field Data Workshop)

Workshop Purpose: to gather valuable feedback from data users and data producers in order to improve the acquisition and use of NASA’s airborne and field data. Workshop Goals: - Identify new ways to improve the discovery, access, and reuse of NASA’s airborne and field data - Intentionally include the airborne community in future planning to ensure the community needs are met - Understand how NASA can better help researchers realize the full value of airborne and field data - Enable Open Source Science and determine what this means for airborne and field data communities

Geoffrey Stano↗

DOE OSTI2025

In anaerobic reactors the microbial community structure depends on feed type, with no “keystone” species tied to COD removal

Two-stage anaerobic digestion (AD) systems provide treatment for high strength wastewater with high stability and performance. Encapsulation technology can intensify AD to facilitate the separation of the solids retention time from the hydraulic retention time (HRT), offering lower HRTs, smaller reactors, and high effluent quality. To support successful deployment, however, the encapsulated community must contain all the needed microorganisms for successful treatment and be flexible enough to treat a variety of wastewaters. Here, a two-stage system was investigated in which microbial cultures were enriched on various high-strength wastewaters in suspended flow-through systems to determine how feed type influenced performance and microbial community structure. The hypothesis was that specific genera, or so-called “keystone species” would positively correlate to organic carbon degradation for a given feed, enabling construction of a well-functioning community for encapsulation. Results showed that the number of total bacteria (as 16S rRNA gene copies) did not correlate to soluble chemical oxygen demand (sCOD) removal, indicating that the community structure and/or members were important for good performance. Results also showed that feed type strongly influenced carbon removal and microbial community structure for 1st-stage fermenting communities, but not 2nd-stage methanogenic communities. In this study, the “core” community members were defined as organisms common to all of either the 1st- or 2nd-stage reactors irrespective of the feed they received and were present in at least 50% of the samples throughout the entire experiment. “Unique” community members were specific to a single feed, and hence, only present in either the 1st- or 2nd-stage reactors receiving that feed. In both 1st- and 2nd-stage communities, only one core genera and no unique genera were positively and significantly correlated to sCOD removal. Verification experiments performed with encapsulated communities showed that organisms identified in flow-through system and correlated with carbon degradation, though not significantly, seemed to be important for performance. Our results suggest that one cannot construct a community containing specific populations in lieu of enrichment. Nevertheless, a single diverse encapsulated anaerobic community should provide good (>80%) carbon removal when fed a variety of influents, if time is provided for enrichment after deployment.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2023

Static Energy Deserves Greater Emphasis in the Meteorology Community

Potential temperature and static energy are both useful quantities for understanding our atmosphere, yet static energy receives much less attention in weather science relative to climate science. Bridging this conceptual gap is important, as there is a pressing need for our communities to work together to understand and predict changing weather patterns in a warming world. Here we provide evidence for this gap in usage in American Meteorological Society journal publications and in introductory textbooks. We then describe key benefits of static energy for explaining basic concepts in atmospheric science. We encourage scientists and educators unfamiliar with static energy to familiarize themselves with the concept and consider incorporating it into their science and teaching.

54 ENVIRONMENTAL SCIENCES↗

NASA NTRSDate not supplied

Exploring our Changing Planet through NASA’s Earth Information Center and Novel Methods for Earth Science Communication

In June 2023, NASA unveiled the Earth Information Center (EIC), an interagency initiative which invites the global community to explore how our planet is changing through interactive installations, captivating visualizations, and novel storytelling. Existing in both physical and virtual space, the EIC serves as a gateway to actionable information collected through an expanding fleet of Earth observing satellites and sensors. The EIC features data driven visualizations, near real-time information, immersive experiences, and curated stories that highlight the applications of publicly available data to address environmental challenges across nine thematic areas: agriculture, biodiversity, disasters, greenhouse gases, air quality, sea level rise, sustainable energy, water resources, and wildfires. Designed by an interdisciplinary team, EIC exhibits are developed to reach a wide base of end-users across multiple learning modalities. During the first year of operation, the inaugural location of the EIC at NASA Headquarters in Washington, DC, welcomed an estimated 24,000 visitors, hosted over 145 tours for domestic and international organizations, supported NASA’s Earth Day 2024 programming, and led six STEM programs for diverse student communities. With lessons learned from the first year of being open to the public and a variety of novel science communication tools in development, the EIC is expanding the mission’s reach by collaborating with museums and visitor centers. Through these collaborations, we aim to inform a broader demographic about the unprecedented changes observed in Earth’s climate and inspire communities to learn more about their one and only home, planet Earth.

Nicole Ramberg-Pihl↗

NASA NTRS2018

Automated Impact Assessment: A New Approach to ISS Payload Operations Anomaly Response

The International Space Station (ISS) Payload Operations and Integration Center (POIC) is undergoing rapid growth as the space station program focuses on science and commercial activities. The ISS is expanding its onboard capabilities to support additional science activities. In parallel, the POIC is expanding the capabilities of our operations tools to support the higher pace of payload activities being executed each week. An effect of these changes is that anomaly resolution has become more challenging. In the event of a real-time system fault, operators are responsible for analyzing telemetry displays, anomaly monitoring tools, documentation, and system models in order to produce failure impacts and recovery strategies. This approach to operations relies on the operator to ingest, process, and analyze information from an array of deterministic sources to provide actionable data on impacted systems and activities. Changing the existing approach of anomaly response is necessary if the ISS community is to succeed in the age of science and commercialization of space. The creation of a tool that captures deterministic technical systems knowledge and integrates existing telemetry, documentation, and planning information will allow the burden of impact assessment to be automated, thereby allowing the operator to focus on non-deterministic tasks, such as recovering failed systems and restoring critical payload operations.

Hall, R. Mason↗

DOE OSTI2024

Panama Rainforest Changes with Experimental Drought (PaRChED): Initial Effects of Partial Throughfall Exclusion on Soil Dynamics in Lowland Forests Across Variation in Rainfall and Soil Fertility

Changes in rainfall are predicted across tropical regions, with effects on nutrient, water, and carbon cycling. This chapter summarizes results from the first two years of a throughfall exclusion experiment in four lowland Panamanian forests that span a 1,000-mm change in rainfall and variation in soil fertility. Soil respiration (i.e., soil carbon dioxide [CO2 ] flux) declined with throughfall exclusion, with a site*season interaction, and the radiocarbon age of respired carbon was older in exclusion versus control plots. The decline in soil CO2 flux could be related to reduced fine root production and soil microbial biomass. Microbial community composition also changed with throughfall exclusion in infertile soils, and soil nutrients accumulated more in exclusion versus control plots during the dry season. The net effects on soil carbon storage will depend on the relative strengths of these effects over time. Continued research could improve predictions of tropical forest-climate feedbacks with changes in precipitation.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2020

Diagnosing and Predicting Mixed-Culture Fermentations with Unicellular and Guild-Based Metabolic Models

Microbiomes are vital to human health, agriculture, and protecting the environment. Predicting behavior of self-assembled or synthetic microbiomes, however, remains a challenge. In this work, we used unicellular and guild-based metabolic models to investigate production of medium-chain fatty acids by a mixed microbial community that is fed multiple organic substrates. Modeling results provided insights into metabolic pathways of three medium-chain fatty acid-producing guilds and identified potential strategies to increase production of medium-chain fatty acids. This work demonstrates the role of metabolic models in augmenting multi-omic studies to gain greater insights into microbiome behavior.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2026

Smart building HVAC control challenge: experience and solutions from the ADRENALIN project

A smart building HVAC control competition crowdsourced and compared algorithms on fair and equal ground using the standardized BOPTEST framework. The competition attracted 138 participants, but only 9% submitted valid solutions for the final stage, highlighting the complexity of advanced HVAC control design. The winning solutions showed significant potential to reduce energy use and cost by shifting demand, without compromising occupant comfort. Across scenarios, thermal energy cost reductions of 36–76% relative to a baseline, were achieved. In peak heat periods, the cost reduction leveraged limited energy use reduction (0–15%), but more significant energy price reduction (34–62%). This shows smart controls' ability to avoid as much as possible consumption during the morning peak hours, when spot prices are tendentially the highest. Hosting the competition has highlighted challenges in creating competitions that both are fair and promotes solutions that are transferable to real life implementation.

BOPTEST↗

DOE OSTI2020

In-field bioreactors demonstrate dynamic shifts in microbial communities in response to geochemical perturbations

Subsurface microbial communities mediate the transformation and fate of redox sensitive materials including organic matter, metals and radionuclides. Few studies have explored how changing geochemical conditions influence the composition of groundwater microbial communities over time. We temporally monitored alterations in abiotic forces on microbial community structure using 1L in-field bioreactors receiving background and contaminated groundwater at the Oak Ridge Reservation, TN. Planktonic and biofilm microbial communities were initialized with background water for 4 days to establish communities in triplicate control reactors and triplicate test reactors and then fed filtered water for 14 days. On day 18, three reactors were switched to receive filtered groundwater from a contaminated well, enriched in total dissolved solids relative to the background site, particularly chloride, nitrate, uranium, and sulfate. Biological and geochemical data were collected throughout the experiment, including planktonic and biofilm DNA for 16S rRNA amplicon sequencing, cell counts, total protein, anions, cations, trace metals, organic acids, bicarbonate, pH, Eh, DO, and conductivity. We observed significant shifts in both planktonic and biofilm microbial communities receiving contaminated water. This included a loss of rare taxa, especially amongst members of the Bacteroidetes, Acidobacteria, Chloroflexi, and Betaproteobacteria, but enrichment in the Fe- and nitrate- reducing Ferribacterium and parasitic Bdellovibrio. These shifted communities were more similar to the contaminated well community, suggesting that geochemical forces substantially influence microbial community diversity and structure. These influences can only be captured through such comprehensive temporal studies, which also enable more robust and accurate predictive models to be developed.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2011

Application of ESE Data and Tools to Air Quality Management: Services for Helping the Air Quality Community use ESE Data (SHAirED)

The goal of this REASoN applications and technology project is to deliver and use Earth Science Enterprise (ESE) data and tools in support of air quality management. Its scope falls within the domain of air quality management and aims to develop a federated air quality information sharing network that includes data from NASA, EPA, US States and others. Project goals were achieved through a access of satellite and ground observation data, web services information technology, interoperability standards, and air quality community collaboration. In contributing to a network of NASA ESE data in support of particulate air quality management, the project will develop access to distributed data, build Web infrastructure, and create tools for data processing and analysis. The key technologies used in the project include emerging web services for developing self describing and modular data access and processing tools, and service oriented architecture for chaining web services together to assemble customized air quality management applications. The technology and tools required for this project were developed within DataFed.net, a shared infrastructure that supports collaborative atmospheric data sharing and processing web services. Much of the collaboration was facilitated through community interactions through the Federation of Earth Science Information Partners (ESIP) Air Quality Workgroup. The main activities during the project that successfully advanced DataFed, enabled air quality applications and established community-oriented infrastructures were: develop access to distributed data (surface and satellite), build Web infrastructure to support data access, processing and analysis create tools for data processing and analysis foster air quality community collaboration and interoperability.

Falke, Stefan↗

NASA NTRS2012

Highlights of Space Weather Services/Capabilities at NASA/GSFC Space Weather Center

The importance of space weather has been recognized world-wide. Our society depends increasingly on technological infrastructure, including the power grid as well as satellites used for communication and navigation. Such technologies, however, are vulnerable to space weather effects caused by the Sun's variability. NASA GSFC's Space Weather Center (SWC) (http://science.gsfc.nasa.gov//674/swx services/swx services.html) has developed space weather products/capabilities/services that not only respond to NASA's needs but also address broader interests by leveraging the latest scientific research results and state-of-the-art models hosted at the Community Coordinated Modeling Center (CCMC: http://ccmc.gsfc.nasa.gov). By combining forefront space weather science and models, employing an innovative and configurable dissemination system (iSWA.gsfc.nasa.gov), taking advantage of scientific expertise both in-house and from the broader community as well as fostering and actively participating in multilateral collaborations both nationally and internationally, NASA/GSFC space weather Center, as a sibling organization to CCMC, is poised to address NASA's space weather needs (and needs of various partners) and to help enhancing space weather forecasting capabilities collaboratively. With a large number of state-of-the-art physics-based models running in real-time covering the whole space weather domain, it offers predictive capabilities and a comprehensive view of space weather events throughout the solar system. In this paper, we will provide some highlights of our service products/capabilities. In particular, we will take the 23 January and the 27 January space weather events as examples to illustrate how we can use the iSWA system to track them in the interplanetary space and forecast their impacts.

Fok, Mei-Ching↗

DOE OSTI2022

The Status and Ambitions of the US Heavy Element Program

The aim of this whitepaper is to highlight the current capabilities and priorities of the US Heavy Element community and to provide the framework for a coordinated advancement of nuclear science from these studies. This is an organized effort to reflect on what has been achieved in the field given the recommendations and initiatives of the 2015 NSAC Long Range Plan, and on what can be realized in the next decade given current and possibly expanded investments. Current investments have positioned the US community to be among the world leaders in studies of the nuclear and chemical properties of the heaviest elements. These include studies of reaction mechanisms, moving us ever closer to the “island of stability”, in spectroscopy, allowing us to better understand nuclear structure at these extreme proton numbers, in chemical behavior, looking to determine how these elements should be placed on the Periodic Table, in performing the first measurements where isotopes are directly identified by their mass numbers, and in laying the foundation for a potential US-led new element discovery experiment. At present, the highest priority of the US Heavy Element community is to capitalize on the current investments by supporting the operations of US facilities at optimal values. These facilities include the Argonne Tandem Linac Accelerator System at Argonne National Laboratory and other Department of Energy facilities such as the 88-Inch Cyclotron at Lawrence Berkeley National Laboratory, which has a dedicated superheavy element program, as well as university laboratories, including Texas A&M University. The High Flux Isotope Reactor at Oak Ridge National Laboratory is crucial to providing the radioactive isotopes required for heavy element science targets. This facility should be supported to provide the actinide materials that are essential for US-based science. Production of stable, rare isotopes for beam material, including 48 Ca, 50 Ti, 54 Cr and 58 Fe, at the Stable Isotope Production and Research Center is critical to continued research in heavy element science and should be a priority. The continued development of targets for heavy element science and retaining US-based expertise is critical for the heavy element community. This is an area that is currently under pressure. For example, the target laboratory at Argonne National Laboratory serves a broad community and is currently under threat due to loss of critical personnel. The skills needed to make targets for nuclear science and develop new targetry methods need to be supported long term at Argonne, Oak Ridge and Lawrence Livermore National Laboratories as well as maintaining the programs at Oregon State University and San José State University as vital pipelines for training students. Advances in theory are the foundation to understand how nuclei behave and to predict those behaviors in new circumstances. Progress in these studies will necessitate continued and new investment and access to high-performance computing. The future health of the heavy element field is dependent on the continuous support of talented early-career professionals at all levels. It is critical that opportunities continue to be created for the next generation to become established in heavy element research so that we can ensure the field is attracting and retaining the best minds for continued success. It is also clear that to ensure diversity of ideas, perspectives and techniques, we need to recruit diverse personnel that are trained at the best facilities. The heavy element community is in support of continued investment to programs with initiatives in diversity, equity, and inclusion. Looking to the next decade of research, support needs to maintain and grow US leadership in heavy element science. Specifically, new investments in state-of-the-art instrumentation will be essential to scientific development of the field and in expanding scientific knowledge. Advances in the next generation of electron cyclotron resonance ion sources, multi-reflection time-of-flight devices, laser spectroscopy, trapping methods and next generation alpha and gamma spectroscopy systems should be prioritized.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

DOE OSTI2024

Seasonality and longer-term development generate temporal dynamics in the Populus microbiome

ABSTRACT Temporal variation in community composition is central to our understanding of the assembly and functioning of microbial communities, yet the controls over temporal dynamics for microbiomes of long-lived plants, such as trees, remain unclear. Temporal variation in tree microbiomes could arise primarily from seasonal (i.e., intra-annual) fluctuations in community composition or from longer-term changes across years as host plants age. To test these alternatives, we experimentally isolated temporal variation in plant microbiome composition using a common garden and clonally propagated plants, and we used amplicon sequencing to characterize bacterial/archaeal and fungal communities in the leaf endosphere, root endosphere, and rhizosphere of two Populus spp. over four seasons across two consecutive years. Microbial community composition differed among seasons and years (which accounted for up to 21% of the variation in microbial community composition) and was correlated with seasonal dissimilarity in climatic conditions. However, microbial community dissimilarity was also positively correlated with time, reflecting longer-term compositional shifts as host trees aged. Together, our findings demonstrate that temporal patterns in tree microbiomes arise from both seasonal fluctuations and longer-term changes, which interact to generate unique seasonal patterns each year. In addition to shedding light on two important controls over the assembly of plant microbiomes, our results also suggest future studies of tree microbiomes should account for background temporal dynamics when testing the drivers of spatial patterns in microbial community composition and temporal responses of plant microbiomes to environmental change. IMPORTANCE Microbiomes are integral to the health of host plants, but we have a limited understanding of the factors that control how the composition of plant microbiomes changes over time. Especially little is known about the microbiome of long-lived trees, relative to annual and non-woody plants. We tested how tree microbiomes changed between seasons and years in poplar (genus Populus ), which are widespread and ecologically important tree species that also serve as important biofuel feedstocks. We found the composition of bacterial, archaeal, and fungal communities differed among seasons, but these seasonal differences depended on year. This dependence was driven by longer-term changes in microbial composition as host trees developed across consecutive years. Our findings suggest that temporal variation in tree microbiomes is driven by both seasonal fluctuations and longer-term (i.e., multiyear) development.

59 BASIC BIOLOGICAL SCIENCES↗