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At least 1,549 records · Page 86

Hindlimb unweighting decreases endothelium-dependent dilation and eNOS expression in soleus not gastrocnemius

We tested the hypothesis that hindlimb unweighting (HLU) decreases endothelium-dependent vasodilation and expression of endothelial nitric oxide synthase (eNOS) and superoxide dismutase-1 (SOD-1) in arteries of skeletal muscle with reduced blood flow during HLU. Sprague-Dawley rats (300-350 g) were exposed to HLU (n = 15) or control (n = 15) conditions for 14 days. ACh-induced dilation was assessed in muscle with reduced [soleus (Sol)] or unchanged [gastrocnemius (Gast)] blood flow during HLU. eNOS and SOD-1 expression were measured in feed arteries (FA) and in first-order (1A), second-order (2A), and third-order (3A) arterioles. Dilation to infusion of ACh in vivo was blunted in Sol but not Gast. In arteries of Sol muscle, HLU decreased eNOS mRNA and protein content. eNOS mRNA content was significantly less in Sol FA (35%), 1A arterioles (25%) and 2A arterioles (18%). eNOS protein content was less in Sol FA (64%) and 1A arterioles (65%) from HLU rats. In arteries of Gast, HLU did not decrease eNOS mRNA or protein. SOD-1 mRNA expression was less in Sol 2A arterioles (31%) and 3A arterioles (29%) of HLU rats. SOD-1 protein content was less in Sol FA (67%) but not arterioles. SOD-1 mRNA and protein content were not decreased in arteries from Gast. These data indicate that HLU decreases endothelium-dependent vasodilation, eNOS expression, and SOD-1 expression primarily in arteries of Sol muscle where blood flow is reduced during HLU.

NASA Discipline Cardiopulmonary↗

Extending unbiased stereology of brain ultrastructure to three-dimensional volumes

OBJECTIVE: Analysis of brain ultrastructure is needed to reveal how neurons communicate with one another via synapses and how disease processes alter this communication. In the past, such analyses have usually been based on single or paired sections obtained by electron microscopy. Reconstruction from multiple serial sections provides a much needed, richer representation of the three-dimensional organization of the brain. This paper introduces a new reconstruction system and new methods for analyzing in three dimensions the location and ultrastructure of neuronal components, such as synapses, which are distributed non-randomly throughout the brain. DESIGN AND MEASUREMENTS: Volumes are reconstructed by defining transformations that align the entire area of adjacent sections. Whole-field alignment requires rotation, translation, skew, scaling, and second-order nonlinear deformations. Such transformations are implemented by a linear combination of bivariate polynomials. Computer software for generating transformations based on user input is described. Stereological techniques for assessing structural distributions in reconstructed volumes are the unbiased bricking, disector, unbiased ratio, and per-length counting techniques. A new general method, the fractional counter, is also described. This unbiased technique relies on the counting of fractions of objects contained in a test volume. A volume of brain tissue from stratum radiatum of hippocampal area CA1 is reconstructed and analyzed for synaptic density to demonstrate and compare the techniques. RESULTS AND CONCLUSIONS: Reconstruction makes practicable volume-oriented analysis of ultrastructure using such techniques as the unbiased bricking and fractional counter methods. These analysis methods are less sensitive to the section-to-section variations in counts and section thickness, factors that contribute to the inaccuracy of other stereological methods. In addition, volume reconstruction facilitates visualization and modeling of structures and analysis of three-dimensional relationships such as synaptic connectivity.

NASA Discipline Neuroscience↗

Characteristics of the Shuttle Orbiter Leeside Flow During A Reentry Condition

A study of the leeside flow characteristics of the Shuttle Orbiter is presented for a reentry flight condition. The flow is computed using a point-implicit, finite-volume scheme known as the Langley Aerothermodynamic Upwind Relaxation Algorithm (LAURA). LAURA is a second-order accurate, laminar Navier-Stokes solver, incorporating finite-rate chemistry with a radiative equilibrium wall temperature distribution and finite-rate wall catalysis. The resulting computational solution is analyzed in terms of salient flow features and the surface quantities are compared with flight data.

Kleb, William L.↗

Evolution of the vestibulo-ocular system

The evolutionary and developmental changes in the eye muscle innervation, the inner ear, and the vestibulo-ocular reflex are examined. Three eye muscle patterns, based on the innervation by distinct ocular motoneurons populations, can be identified: a lamprey, an elasmobranch, and a bony fish/tetrapod pattern. Four distinct patterns of variation in the vestibular system are described: a hagfish pattern, a lamprey pattern, an elasmobranch pattern, and a bony fish/tetrapod pattern. Developmental data suggest an influence of the hindbrain on ear pattern formation, thus potentially allowing a concomitant change of eye muscle innervation and ear variation. The connections between the ear and the vestibular nuclei and between the vestibular nuclei and ocular motoneurons are reviewed, and the role of neurotrophins for pattern specification is discussed. Three patterns are recognized in central projections: a hagfish pattern, a lamprey pattern, and a pattern for jawed vertebrates. Second-order connections show both similarities and differences between distantly related species such as lampreys and mammals. For example, elasmobranchs lack an internuclear system, which is at best poorly developed in lampreys. It is suggested that the vestibulo-ocular system shows only a limited degree of variation because of the pronounced functional constraints imposed on it.

Non-NASA Center↗

Coupling of fingertip somatosensory information to head and body sway

Light touch contact of a fingertip with a stationary surface can provide orientation information that enhances control of upright stance. Slight changes in contact force at the fingertip provide sensory cues about the direction of body sway, allowing attenuation of sway. In the present study, we asked to which extent somatosensory cues are part of the postural control system, that is, which sensory signal supports this coupling? We investigated postural control not only when the contact surface was stationary, but also when it was moving rhythmically (from 0.1 to 0.5 Hz). In doing so, we brought somatosensory cues from the hand into conflict with other parts of the postural control system. Our focus was the temporal relationship between body sway and the contact surface. Postural sway was highly coherent with contact surface motion. Head and body sway assumed the frequency of the moving contact surface at all test frequencies. To account for these results, a simple model was formulated by approximating the postural control system as a second-order linear dynamical system. The influence of the touch stimulus was captured as the difference between the velocity of the contact surface and the velocity of body sway, multiplied by a coupling constant. Comparison of empirical results (relative phase, coherence, and gain) with model predictions supports the hypothesis of coupling between body sway and touch cues through the velocity of the somatosensory stimulus at the fingertip. One subject, who perceived movement of the touch surface, demonstrated weaker coupling than other subjects, suggesting that cognitive mechanisms introduce flexibility into the postural control scheme.

NASA Discipline Neuroscience↗

A dynamical model for reflex activated head movements in the horizontal plane

We present a controls systems model of horizontal-plane head movements during perturbations of the trunk, which for the first time interfaces a model of the human head with neural feedback controllers representing the vestibulocollic (VCR) and the cervicocollic (CCR) reflexes. This model is homeomorphic such that model structure and parameters are drawn directly from anthropomorphic, biomechanical and physiological studies. Using control theory we analyzed the system model in the time and frequency domains, simulating neck movement responses to input perturbations of the trunk. Without reflex control, the head and neck system produced a second-order underdamped response with a 5.2 dB resonant peak at 2.1 Hz. Adding the CCR component to the system dampened the response by approximately 7%. Adding the VCR component dampened head oscillations by 75%. The VCR also improved low-frequency compensation by increasing the gain and phase lag, creating a phase minimum at 0.1 Hz and a phase peak at 1.1 Hz. Combining all three components (mechanics, VCR and CCR) linearly in the head and neck system reduced the amplitude of the resonant peak to 1.1 dB and increased the resonant frequency to 2.9 Hz. The closed loop results closely fit human data, and explain quantitatively the characteristic phase peak often observed.

NASA Discipline Neuroscience↗

Digital Particle Image Velocimetry (DPIV) Used for Space-Time Correlations in Nozzle Flow

An optical measurement technique known as Digital Particle Image Velocimetry (DPIV) was used previously to characterize the first- and second-order statistical properties of both cold and hot jet flows from externally mixed nozzles in NASA Glenn Research Center's Nozzle Acoustic Test Rig. In this technique, an electronic camera records particles entrained in a flow as a laser light sheet is pulsed at two instances in time. Correlation processing of the recorded particle image pairs yields the two-component velocity field across the imaged plane of the flow. The information acquired using DPIV is being used to improve our understanding of the decay of turbulence in jet flows-a critical element for understanding the acoustic properties of the flow. Recently, two independent DPIV systems were installed in Glenn's Small Hot Jet Acoustic Rig, enabling multiplane correlations in time and space. The data were collected over a range of different Mach numbers and temperature ratios. DPIV system 1 was fixed to a large traverse rig, and DPIV system 2 was mounted on a small traverse system mounted on the large traverse frame. The light sheets from the two DPIV systems were aligned to lie in the same axial plane, with DPIV system 2 being independently traversed downstream along the flow direction. For each measurement condition, the DPIV systems were started at a fully overlapping orientation. A polarization separation technique was used to avoid cross-talk between the two systems. Then, the DPIV systems fields were shifted axially apart, in successively increasing steps. The downstream DPIV system 2 was triggered at a short time delay after the upstream DPIV system 1, where the time delay was proportional to the convective flow velocity in the shear layer of the jet flow and the axial separation of the two DPIV systems. The acquired data were processed to obtain the instantaneous velocity vector maps over a range of time delays and spatial separations. The velocity fields from the different DPIV systems were then cross-correlated to determine the degree of correlation remaining in the flow as the downstream convection distance was increased. The new data provide Lagrangian measurements of the convective turbulent structures in the shear layer of an exhaust nozzle. These measurements, obtained in both cold and hot flows, will be used to validate and correct models for space-time velocity correlations-long a missing key to predicting jet noise.

Wernet, Mark P.↗

The Parametrized Post-Newtonian Gravitational Redshift

A derivation of the gravitational redshift effect to order c ^(-4) is presented. The calculation isperformed within the framework of the parametrized post-Newtonian formalism for analyzing metrictheories of gravity, which includes corrections to second- order in the Newtonian potential,gravitomagnetic contributions, and preferred-frame terms. We briefly discuss how to generalize ourresults to include possible violations of local Lorentz invariance or local position invariance which canarise in nonmetric theories. Our results are useful for analyzing possible new redshift experimentswhich may be sensitive to second-order effects, such as a close solar flyby mission.

Krisher, T. P.↗

The Effects of Noise on Polarimetric SAR Data

Polarimetric SAR data can provide a great deal of information about the scattering behavior of the surface under observation. Polarimetric SAR systems often measure the scattering matrices of the areas under observation in linear polarizations (H and V). From the scattering matrix commonly used forms such as the covariance matrix and the Stokes matrix can be easily derived. Other measures derived from polarimetric SAR data include correlation coefficients between scattering matrix terms and the mode and variance of phase differences between scattering matrix terms. The effects of additive system noise on these measurements is not often considered in the literature on this subject. In this paper, the effects of additive system noise on measurements derived from polarimetric SAR data will be examined. It will be shown how first-order noise effects can be removed and how second-order noise effects can be reduced for some measurements...

Freeman, A.↗

A Parallel Incompressible Navier-Stokes Solver with Multigrid Iterations

We developed a parallel, numerically accurate and stable, and computationally efficient finate-difference incompressible Navier-Stokes (N-S) fluid flow solver. The solver runs on both sequential and massively parallel computers. The numerical method used here is a second-order projection method on a staggered grid. The code is highly modular and it can be used either as a stand-alone flow solver and or a template code which can be adapted or expanded to a specific application. Numerical results and parallel performances of our code on Intel Delta and Paragon are reported.

Navier-Stokes solver↗

Method for suppressing noise in measurements

Techniques of combining separate but correlated measurements to form a second-order or higher order correlation function to suppress the effects of noise in the initial condition of a system capable of retaining memory of an initial state of the system with a characteristic relaxation time. At least two separate measurements are obtained from the system. The temporal separation between the two separate measurements is preferably comparable to or less than the characteristic relaxation time and is adjusted to allow for a correlation between two measurements.

Carson, Paul J.↗

Resonance Cases and Small Divisors in a Third Integral of Motion

In this paper a general discussion of the resonance cases in an axially-symmetric potential field is presented, when the unperturbed frequencies in the radial and z direction have a rational ratio. The general form of the third integral is not valid in these cases because of the appearance of divisors of the form (m(exp 2)P-n(exp 2)Q), which become zero in the resonance cases. However, a new isolating integral of the unperturbed case is available, and this can be used to construct a third integral in the form of a power series and eliminate all secular terms. Three cases are distinguished, (alpha) m+n>4, (beta) m+n=4, and (gamma) m+n<4. In the first case the orbits are rather similar to those of the general irrational case. In the third case th e orbits show a quite peculiar character, which, however, can be explained rather accurately by a first-order theory of the third integral. Numerical integrations were made for the cases P=16Q, 4P=9Q, and P=4Q. The third integral, given in first- or second-order approximation, is rather well conserved. Case beta and the cases of small divisors, when m(exp 2)P-n(exp 2)Q is near zero but not equal to zero, are discussed in Paper II.

Contopoulos, George↗

An analysis of digital phase-locked loops

This report focuses on second-order digital phase-locked loops (DPLLs) with uniformly sampled input, an amplitude-insenstive phase extractor and a conventional loop filter.

Thomas, J. B.↗

Higher-Order Ionospheric Effects on the GPS Observables and Means of Modeling Them

Based on realistic modeling of the electron density of the ionosphere and using a dipole moment approximation for the earth magnetic field, we are able to estimate the effect of the ionosphere on the GPS signal for a ground user. Our study implies reduction of the second-order ionospheric error to less than 2 mm on the average and, therefore, potential improvement of orbit determination and baseline solutions.

ionosphere↗

Resolution requirements for high-speed droplet impact along a rigid wall

The mechanical and thermal loads that are produced on high-speed projectiles from a droplet are key in the projectile development.To accurately predict the pressure produced along the wall surface during impact, it is important to correctly model the air/water interface of the droplet. Novel modeling approaches such as the Phase-Field enable control of the (numerical) interface thickness. However, the interplay between the grid resolution (including potential adaptive mesh refinement) and the interface thickness is unclear. In this work, the resolution requirements for numerical simulations of a two-dimensional cylindrical water droplet impacting a rigid wall at Mach four are computationally investigated. Numerical simulations are performed using a second-order accurate scheme with adaptive mesh refinement and a Phase-Field interface model. The choice of numerical interface thickness and resolution parameters affects the maximum pressure and location of the maximum pressure along the wall. The overall maximum pressure does not converge even at high resolutions. The number of levels and thickness (number of cells across the interface) play a role in obtaining a well behaved solution.

Burrell, E.↗

Time correlations from steady-state expectation values

Recovering properties of correlation functions is typically challenging. On one hand, experimentally, it requires measurements with a temporal resolution finer than the system's dynamics. On the other hand, analytical or numerical analysis requires solving the system evolution. Here, we use recent results of quantum metrology with continuous measurements to derive general lower bounds on the relaxation and second-order correlation times that are both easy to calculate and measure. These bounds are based solely on steady-state expectation values and their derivatives with respect to a control parameter, and can be readily extended to the autocorrelation of arbitrary observables. We validate our method on two examples of critical quantum systems: a critical driven-dissipative resonator, where the bound matches analytical results for the dynamics, and the infinite-range Ising model, where only the steady state is solvable and thus the bound provides information beyond the reach of existing analytical approaches. Our results can be applied to experimentally characterize ultrafast systems, and to theoretically analyze many-body models with dynamics that are analytically or numerically hard.

Górecki, Wojciech [INFN, Pavia]↗

Time correlations from steady-state expectation values

Recovering properties of correlation functions is typically challenging. On one hand, experimentally, it requires measurements with a temporal resolution finer than the system's dynamics. On the other hand, analytical or numerical analysis requires solving the system evolution. Here, we use recent results of quantum metrology with continuous measurements to derive general lower bounds on the relaxation and second-order correlation times that are both easy to calculate and measure. These bounds are based solely on steady-state expectation values and their derivatives with respect to a control parameter, and can be readily extended to the autocorrelation of arbitrary observables. We validate our method on two examples of critical quantum systems: a critical driven-dissipative resonator, where the bound matches analytical results for the dynamics, and the infinite-range Ising model, where only the steady state is solvable and thus the bound provides information beyond the reach of existing analytical approaches. Our results can be applied to experimentally characterize ultrafast systems, and to theoretically analyze many-body models with dynamics that are analytically or numerically hard.

Górecki, Wojciech [INFN, Pavia]↗