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At least 1,081 records · Page 60

Electrophoretic purification of cells in space - Evaluation of results from STS-3

The procedure and results of Electrophoresis Equipment Verification Test, designed to examine electrophoretic behavior of animal cells is suspension more concentrated than possible on earth and flown on the Shuttle flight STS-3, were discussed. Ground-based laboratory values of electrophoretic mobilities of a mixture of human and rabbit aldehyde-fixed red blood cells (RBC) were compared with those recorded at 11 minute intervals on the Shuttle STS-3. RBC migration and separation observed through photographic records were not as expected. However, cell mobilities and migrating band profiles were consistent with the results of laboratory simulation experiments. It was concluded that zero G electrophoresis of very high concentrations (1 x 10 to the 9th) is possible and similar to electrophoresis of normal cell concentrations on earth.

Sarnoff, B. E.↗

Approximating a finite element model of a hybrid deployable truss by a discrete mass model

The NASTRAN finite element model of the MSFC/hybrid deployable truss is approximated by a model consisting of four bodies serially connected by a spring hinge suspension. The truss is decomposed into four serially connected modules determining the total mass and rotational inertias for a rigid body with its mass center on the axis of the truss for each module. A five-body cantilever truss model is developed along with an undamped form of this model. Spring coefficient values are generated to match eigenvalues of the latter model with those of the NASTRAN model. Damping coefficients are then determined that provide n percent of critical damping for the model.

Chichester, F. D.↗

Containerless processing technology

A partial description of the technology, hardware, and facilities developed by NASA in support of space-based containerless processing experiments in the early 1970s is presented, along with recent results. Supercooling was necessary, at a rate of 500 K over a 400 ft span, in early drop tower and molten sample experiments. Placing an electromagnetic levitation coil/chamber at the top of the tube avoided contamination of the sample by guide wires. Recent tests have been performed in attempts to produce metastable bulk A-15 Nb3Ge. The use of copper coils for levitation is suspected to have induced stirring motions in samples. Aerodynamic levitation, needed for nonconducting materials, has been employed to process glass beads at temperatures over 1000 K. An electromagnetic levitation device used to process a gram of BeO on a sounding rocket flight will be carried on a Shuttle flight, as will an acoustic suspension system.

Oran, W. A.↗

High accuracy pointing for earth observation experiments

An evaluation of mission requirements for postulated earth observation orbital payload experiments has indicated that high accuracy pointing and stabilization must be provided for adequate performance. A computer simulation has been conducted to determine the capabilities of an auxiliary pointing system which employs a combination of conventional gimbals and magnetic bearing actuators. This Annular Suspension and Pointing System (ASPS) is found to be a viable means of providing the high slew rates associated with landmark tracking from low orbit, while simultaneously achieving the high degree pointing and stabilization dictated by science payload requirements.

Keckler, C. R.↗

A mathematical simulation model of the CH-47B helicopter, volume 1

A nonlinear simulation model of the CH-47B helicopter was adapted for use in the NASA Ames Research Center (ARC) simulation facility. The model represents the specific configuration of the ARC variable stability CH-47B helicopter and will be used in ground simulation research and to expedite and verify flight experiment design. Modeling of the helicopter uses a total force approach in six rigid body degrees of freedom. Rotor dynamics are simulated using the Wheatlely-Bailey equations including steady-state flapping dynamics. Also included in the model is the option for simulation of external suspension, slung-load equations of motion.

Weber, J. M.↗

Modular design attitude control system

A sequence of single axismodels and a series of reduced state linear observers of minimum order are used to reconstruct inaccessible variables pertaining to the modular attitude control of a rigid body flexible suspension model of a flexible spacecraft. The single axis models consist of two, three, four, and five rigid bodies, each interconnected by a flexible shaft passing through the mass centers of the bodies. Modal damping is added to each model. Reduced state linear observers are developed for synthesizing the inaccessible modal state variables for each modal model.

Chichester, F. D.↗

Measurement of the inertial constants of a rigid or flexible structure of arbitrary share through a vibration test

The inertial constants of an aircraft rocket, or of any other structure, are defined without materializing any rotating axis. The necessary equipment is very similar to that used normally for ground vibration tests. An elastic suspension is used to obtain the total natural modes corresponding to the motions of the structure as a solid. From the measurements of the generalized masses of these modes it is possible to compute the inertial constants: (1) center of inertia; (2) tensor of inertia; and (3) mass. When the structure is not strictly rigid a purification process, based on the mean square method makes it possible to rigidify it at the price of some approximations and a few more measurements. Eventual additional masses, that are not parts of the structure, can be taken into account.

Engrand, D.↗

The rheology of icy satellites

High-temperature creep in orthoenstatite under conditions of controlled oxygen fugacity was studied. It was found that creep was conttrolled by the extremely thin layer of SiO2 which wetted the grain boundaries. Slight reduction of the (Mg, Fe)SiO3 enstatite during hot pressing produced microscopic particles of Fe and the thin film of intergranular SiO2. This result highlights another complication in determining the flow properties of iron bearing silicates which constitute the bulk of terrestrial planets and moons. The Phenomenon may be important in the ductile formation of any extraterrestrial body which is formed in a reducing environment. The rheology of dirty ice was studied. This involves micromechanical modeling of hardening phenomena due to contamination by a cosmic distribution of silicate particles. The larger particles are modeled by suspension theory. In order to handle the distribution of particles sizes, the hardening is readed as a critical phenomenon, and real space renormalization group techniques are used. Smaller particles interact directly with the dislocations. The particulate hardening effect was studied in metals. The magnitude of such hardening in ice and the defect chemistry of ice are studied to assess the effects of chemical contamination by methane, ammonia, or other likely contaminants.

Sammis, C. G.↗

An Assessment of Integrated Flywheel System Technology

The current state of the technology in flywheel storage systems and ancillary components, the technology in light of future requirements, and technology development needs to rectify these shortfalls were identified. Technology efforts conducted in Europe and in the United States were reviewed. Results of developments in composite material rotors, magnetic suspension systems, motor/generators and electronics, and system dynamics and control were presented. The technology issues for the various disciplines and technology enhancement scenarios are discussed. A summary of the workshop, and conclusions and recommendations are presented.

Keckler, C. R.↗

Electrical/electronics working group summary

The electrical/electronics, technology area was considered. It was found that there are no foreseeable circuit or component problems to hinder the implementation of the flywheel energy storage concept. The definition of the major component or technology developments required to permit a technology ready date of 1987 was addressed. Recommendations: motor/generators, suspension electronics, power transfer, power conditioning and distribution, and modeling. An introduction to the area of system engineering is also included.

Schoenfeld, A. D.↗

European development experience on energy storage wheels for space

High speed fiber composite rotors suspended by contactless magnetic bearings were produced. European industry has acquired expertise in the study and fabrication of energy storage wheels and magnetic suspension systems for space. Sufficient energy density performance for space viability is being achieved on fully representative hardware. Stress cycle testing to demonstrate life capability and the development of burst containment structures remains to be done and is the next logical step.

Robinson, A. A.↗

Overview of magnetic bearing control and linearization approaches for annular magnetically suspended devices

An overview of magnetic bearing control and linearization approaches which have been considered for annular magnetically suspended devices is presented. These devices include the Annular Momentum Control Device and the Annular Suspension and Pointing System. Two approaches were investigated for controlling the magnetic actuator. One approach involves controlling the upper and lower electromagnets differentially about a bias flux. The bias flux can either be supplied by permanent magnets in the magnetic circuit or by bias currents. In the other approach, either the upper electromagnet or the lower electromagnet is controlled depending on the direction of force required. One advantage of the bias flux is that for small gap perturbations about a fixed operating point, the force-current characteristic is linear. Linearization approaches investigated for individual element control include an analog solution of the nonlinear electromagnet force equation and a microprocessor-based table lookup method.

Groom, N. J.↗

Purification and cultivation of human pituitary growth hormone secreting cells

A multiphase study was conducted to examine the properties of growth hormone cells. Topics investigated included: (1) to determine if growth hormone (GH) cells contained within the rat pituitary gland can be separated from the other hormone producing cell types by continuous flow electrophoresis (CFE); (2) to determine what role, if any, gravity plays in the electrophoretic separation of GH cells; (3) to compare in vitro GH release from rat pituitary cells previously exposed to microgravity conditions vs release from cells not exposed to microgravity; (4) to determine if the frequency of different hormone producing pituitary cell types contained in cell suspensions can be quantitated by flow cytometry; and (5) to determine if GH contained within the human post mortem pituitary gland can be purified by CFE. Specific experimental procedures and results are included.

Hymer, W. C.↗

The salutary effect of dietary calcium on bone mass in a rat model of simulated weightlessness

Whether supplementation of dietary calcium reduces the differences in bone mass of unweighed limbs and normally weighted limbs, and whether parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D (1,25(OH)2D) respond differently to dietary calcium in unweighted animals in comparison with pair-fed controls was studied. The hind limbs of rats were unweighted by a tail suspension method and diets containing 0.1% to 2.4% calcium. After 2 weeks serum calcium, phosphorus, PTH and 1,25(OH)2D intestinal calcium transport were determined and bone mass, ash weight, and calcium in the tibia, L-1 vertebra, and humerus were measured. No significant differences in body weights were observed among the various groups. Suspended rats maintained constant levels of serum calcium and phosphate over the wide range of dietary calcium. Serum PTH and 1,25(OH)2D and intestinal calcium transport fell as dietary calcium was increased. Bone calcium in the tibia and vertebra from suspended rats remained less than that from pair-fed control. It is suggested that although no striking difference between suspended and control animals was observed in response to dieteary calcium, increasing dietary calcium may reduce the negative impact of unloading on the calcium content of the unweighted bones. The salutary effect of high dietary calcium appears to be due to inhibition of bone resorption rather than to stimulation of bone formation.

Bikle, D. D.↗

Quantitative Alterations in the Function of Bone Forming Cells Due to Reduced Weight Bearing

Rats subjected to spaceflight or suspended in a non-weight bearing position for 2 to 3 weeks, show a significant reduction in new bone formation. This reduction is associated with a decrease in akaline phosphatase activity in the differentiated osteoblast population. Those cells in the siaphyseal region of bone are more affected than the same cell type in metaphyseal bone. Measurements of alkaline phosphate activity in specific regions of bone, and the autoradiographic localization of H(3) proline in bone forming areas are described. Concomitant with decreased bone matrix synthesis, the osteoblast population also demonstrate changes in the Golgi/lysosomal complex as a result of whole animal suspension. Morphometric techniques are being applied for quantitation of the lysosomal population and the percentage of lysosomal or Golgi bodies containing acid phosphatase activity.

Doty, S. B.↗

Changes in bone structure and metabolism during simulated weightlessness: Endocrine and dietary factors

The role of vitamin D, PTH and corticosterone in the skeletal alterations induced by simulated weightlessness was examined. The first objective was to determine if changes in the serum concentrations of Ca, P sub i, osteocalcin, 25-OH-D, 24,25(OH)2D or 1,25(OH)2D also occur following acute skeletal unloading. Animals were either suspended or pair fed for 2, 5, 7, 10, 12 and 15 days and the serum concentrations of Ca, P sub i, osteocalcin and the vitamin D metabolites measured. Bone histology was examined at day 5 after suspension. Acute skeletal unloading produced a transient hypercalcemia, a significant fall in serum osteocalcin and serum 1,25(OH)2D, a slight rise in serum 24,25(OH)2D, but did not affect the serum concentrations of P sub i or 25-OH-D. At the nadir in serum 1,25(OH)2D serum osteocalcin was reduced by 22%, osteoblast surface by 32% and longitudinal bone growth by 21%.

Halloran, B. P.↗