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WSF PISA example for SAFER pilot

On October 15, 2021, the Radioactive and Hazardous Waste Management Facility Manager declared a Potential Inadequacy in the Safety Analysis (PISA) due to a new information relating to an error discovered in calculation AB-WSF-20-002. Calculation AB-WSF-20-002 is used to determine source terms and heat release rates for vehicle and aircraft impacts with fuel fires involving TRU waste containers. The source term for an aircraft’s impact has different source terms factors (i.e., DR, ARF, RF, LPF) that are applied to multiple categories of drums impacted in the accident scenario based on the physical stresses of the aircraft impact and involvement in the subsequent fuel pool fire. The source terms of the multiple categories of affected drums are summed to determine the final source term for the accident scenario. Certain categories of affected drums are modeled to lose their lid, causing material to eject and burn unconfined on the ground. For these specific categories, a lower ARF value was incorrectly applied in AB-WSF-20-002 (i.e., an ARF value of 1E-3 was used instead of the DOE-STD-5506-2007 directed ARF value of 1E-2). For these categories, the ARF is applied to a relatively small number of low-activity drums (i.e., 17 drums at 2.9 PE-Ci each) compared to the total number of drums affected (i.e., 166 drums totaling 621.8 PE-Ci). Therefore, this error is not expected to significantly increase final dose for the accident scenario. No immediate actions or compensatory measures are required to maintain the facility in a safe condition.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

A Study of Flame Propagation on Water-Mist Laden Gas Mixtures in Microgravity

The use of water mists (very fine water sprays) for fire suppression is currently receiving increased attention as a replacement technology for halogen-based chemical agents-such as Halon 1301 (CF3Br)--the manufacturing of which has been banned by the Montreal Protocol due to their high ozone depletion potential. Water mist technology has been found effective for a wide range of applications such as Class B pool fires, shipboard machinery, aircraft cabins, computers, and electronic equipment. There are five distinct mechanisms by which water droplets may interact with a flame. First, the high enthalpy of vaporization of water (2450 kJ/kg) leads to heat removal from the flame front as the liquid droplets turn to steam. Second, as water vaporizes its volume increases approximately three orders of magnitude, which leads to the dilution of the oxygen and vaporized fuel required to maintain the flame. The third effect is the recombination of H-atoms and other radicals on the droplet surface. A fourth effect of water mists in fires is the retardation of surface propagation rates due to the wetting of walls and surfaces. The last potential impact of fine water mists affects the radiative propagation of the fire by forming an optically thick barrier to infrared radiation which prevents ignition of the unburned regions. Unfortunately, little fundamental information exists on the interaction of a flame with a water mist. To date, there is no widely accepted interpretation of the critical concentration of droplets required to suppress a flame or of the fundamental mechanisms involved in flame extinguishment by water mists. One of the main obstacles to obtaining such understanding is the difficulty of providing a simple, well-defined experimental setup for the flame front/water mist interaction. Some of the difficulty stems from the problem of generating, distributing and maintaining a homogeneous concentration of droplets throughout a chamber while gravity depletes the concentration and alters the droplet size by coalescence and agglomeration mechanisms. Experiments conducted in the absence of gravity provide an ideal environment to study the interaction of water mists and flames by eliminating these distorting effects. In addition, microgravity eliminates the complex flow patterns induced between the flame front and the water droplets. The long duration and quality of microgravity in space flights provide the required conditions to perform the setup and monitoring of flame suppression experiments. Consequently, a series of experiments have been identified to be performed on the Combustion Module (CM-2) in the Space Shuttle. These consist of measuring the extinguishing capability of a water mist on a premixed flame propagating along a tube. These experiments should provide the necessary data to obtain further understanding of the water mist suppression phenomena that can be later used to design and manufacture appropriate fire suppression systems. In preparation for the orbital flights, experiments have been conducted on low-gravity ground facilities to obtain the preliminary data necessary to define the scientific objectives and technical issues of the spacecraft experiments.

Abbud-Madrid, A.↗

Model Data Archive Associated with Manuscript "Fire-altered Carbon Pools Create Disturbance Memory in Stream Dissolved Organic Carbon"

This data package supports the publication “Fire-altered Carbon Pools Create Disturbance Memory in Stream Dissolved Organic Carbon” by Li et al. (2026). The package contains processed model inputs, configuration files, restart files, simulation outputs, scripts, and visualization products used to evaluate post-fire dissolved organic carbon (DOC) dynamics in the Naches River Watershed, Washington, USA, following the 2021 Schneider Springs Fire. The modeling workflow couples ELM-BGC, the biogeochemistry-enabled Energy Exascale Earth System Model Land Model; ATS, the Advanced Terrestrial Simulator for integrated surface-subsurface hydrology; and PFLOTRAN, a reactive transport model for multicomponent aqueous geochemistry. Together, these models simulate how wildfire-induced changes in vegetation, litter, coarse woody debris, and soil organic matter influence DOC production, transport, and reaction from burned hillslopes to stream networks. The archive includes preprocessed meteorological, geospatial, hydrologic, and biogeochemical forcing data; ELM-BGC-derived DOC source terms; ATS mesh files; PFLOTRAN reactive-transport inputs; model configuration files; spin-up and transient restart files; watershed-scale diagnostic outputs; stream concentration time series; and figures or visualization files used to inspect and reproduce key results. File types include Hierarchical Data Format 5 (HDF5) files for gridded forcing and model-coupling data, model input and configuration files for ELM-BGC, ATS, and PFLOTRAN, restart and simulation-output files generated by the modeling workflow, tabular or time-series diagnostic outputs, scripts for post-processing and figure generation, and image or visualization products associated with the manuscript. Use of the package depends on the intended task. Re-running the simulations requires the relevant modeling software, including ELM-BGC, ATS, and PFLOTRAN as ATS's geochemical engine. Inspecting outputs and reproducing figures requires Python with scientific plotting libraries such as Matplotlib, and three-dimensional model outputs may be viewed with ParaView. Geographic information system files or maps may be inspected with ArcGIS Pro or comparable GIS software. The data package is intended to enable traceability, reuse, and partial reproduction of the coupled land-to-watershed hydro-biogeochemical modeling workflow used to test how wildfire disturbance affects terrestrial carbon pools and downstream DOC dynamics.

ATS↗

Benchmarking MELCOR's NAC Package to ABCOVE Test AB7

This report presents analyses of the AB7 ABCOVE sodium spray fire experiment with the MELCOR code. This code simulates the progression of accident events for analysis and auditing purposes of nuclear facilities during accident conditions. Historically, the ABCOVE experiments have contributed to the validation of aerosol physics and related phenomena. Given advancements in sodium-cooled reactor designs, characterization of the sodium spray combustion may further the review and validation of newly incorporated sodium properties and physics packages, namely, the sodium equations of state (EOS) and the sodium combustion (NAC) package within MELCOR. Previously, the AB5 and AB6 experiments were analyzed with and without the NAC package. This work builds on the previous analyses with a demonstration of the current code capabilities of MELCOR with a more mild Na spray and pool fire scenario.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Accurate Compression of Tabulated Chemistry Models with Partition of Unity Networks

Tabulated chemistry models are widely used to simulate large-scale turbulent fires in applications including energy generation and fire safety. Tabulation via piecewise Cartesian interpolation suffers from the curse-of-dimensionality, leading to a prohibitive exponential growth in parameters and memory usage as more dimensions are considered. Artificial neural networks (ANNs) have attracted attention for constructing surrogates for chemistry models due to their ability to perform high-dimensional approximation. However, due to well-known pathologies regarding the realization of suboptimal local minima during training, in practice they do not converge and provide unreliable accuracy. Partition of unity networks (POUnets) are a recently introduced family of ANNs which preserve notions of convergence while performing high-dimensional approximation, discovering a mesh-free partition of space which may be used to perform optimal polynomial approximation. In this work, we assess their performance with respect to accuracy and model complexity in reconstructing unstructured flamelet data representative of nonadiabatic pool fire models. Our results show that POUnets can provide the desirable accuracy of classical spline-based interpolants with the low memory footprint of traditional ANNs while converging faster to significantly lower errors than ANNs. For example, we observe POUnets obtaining target accuracies in two dimensions with 40 to 50 times less memory and roughly double the compression in three dimensions. We also address the practical matter of efficiently training accurate POUnets by studying convergence over key hyperparameters, the impact of partition/basis formulation, and the sensitivity to initialization.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Release of Contaminants from Burning Liquids and Solids

This report details model development, theory, and a literature review focusing on the emission of contaminants on solid substrates in fires. This is the final report from a 2-year Nuclear Safety Research and Development (NSRD) project. The work represents progress towards a goal of having modeling and simulation capabilities that are sufficiently mature and accurate that they can be utilized in place of physical tests for determining safe handling practices. At present, the guidelines for safety are largely empirically based, derived from a survey of existing datasets. This particular report details the development, verification and calibration of a number of code improvements that have been implemented in the SIERRA suite of codes, and the application of those codes to three different experimental scenarios that have been subject of prior tests. The first scenario involves a contaminated PMMA slab, which is exposed to heat. The modeling involved a novel method for simulating the viscous diffusion of the particles in the slab. The second scenario involved a small pool fire of contaminated combustible liquid mimicking historical tests and finds that the release of contaminants has a high functionality with the height of the liquid in the container. The third scenario involves the burning of a contaminated tray of shredded cellulose. A novel release mechanism was formulated based on predicted progress of the decomposition of the cellulose, and while the model was found to result in release that can be tuned to match the experiments, some modifications to the model are desirable to achieve quantitative accuracy.

54 ENVIRONMENTAL SCIENCES↗

Structural uncertainty assessment for fire-engulfed objects in crosswind: Establishing credibility for a multiphysics wall-modeled large-eddy simulation paradigm

A structural uncertainty validation study for a large-scale, fire-engulfed, elevated object subjected to crosswind is presented to establish the credibility of a high-fidelity, low-Mach, turbulent reacting flow wall-modeled large-eddy simulation (WMLES) approach that includes multiphysics coupling to participating media radiation and conjugate heat transfer. To establish that WMLES can accurately predict surface quantities including drag and pressure coefficient in the low-Mach crosswind regime, a foundational elevated isothermal cylinder validation case is presented at a similar gap-to-diameter ratio of 0.25, spanning the subcritical to supercritical drag regime (Re 𝐷 = 1.1 × 10 5 and 4.3 × 10 5 , respectively). Here, this study exercised both static and dynamic coefficient LES (Smagorinsky and 𝑘 sgs ) with both local and exchange-based velocity sampling. Results showcase that the drag crisis (or the sudden drop in drag coefficient at increased Re 𝐷 ) is well captured when using an exchange-based dynamic coefficient WMLES methodology, while noting lack of mesh convergence and overall drag and pressure coefficient predictively when using a static coefficient, local velocity sampling WMLES. For the 𝒪⁡(10) m JP-8 liquid pool fire crosswind validation study presented, two experimental crosswind configurations (2 m/s and 9.5 m/s) are showcased for a fire-engulfed mock fuselage roughly 4 m in diameter. Using the best model-form practices identified in the isothermal study, dynamic coefficient 𝑘 sgs exchange-based WMLES fire validation findings demonstrate accurate peak irradiation and skin temperature predictions as a function of crosswind magnitude. Excessive yaw in the low-crosswind fuselage configuration, consistent with experimental findings, captured a significant predicted asymmetry in flame attachment and heat flux toward the downwind cylindrical cap—indicative of axial vortex structures transporting the flame along the upper and lower fuselage leeward surface. All fire mesh resolution simulations captured the experimental finding that as crosswind increased, predicted flame shape and peak irradiation magnitude onto the fuselage transitioned from a windward to a leeward cylinder location due to the migration of the upper- to lower-shear fuel/air mixing layer thereby demonstrating the novelty, significance, and credibility of this high-fidelity WMLES reacting flow framework.

Domino, Stefan Paul [Sandia National Laboratories ↗

Investigation of fuel film formation and soot emissions in a GDI engine during cold-start with Split-injection strategies

This study investigates the impact of fuel-film formation on engine-out soot emissions in a gasoline direct injection (GDI) engine under cold-start conditions. Split-injection strategies were applied by varying the number of injections, injection duration, and total fuel quantity to affect wall wetting and control the average in-cylinder equivalence ratio. A combined experimental and numerical approach was employed to analyze fuel-film deposition, combustion efficiency, and engine-out soot and unburnt hydrocarbons (UHC) emissions. In particular, fuel film distribution estimated by means of non-reacting, 3-D, computational fluid dynamics (CFD) simulations, together with experimentally measured soot data, were used to investigate fuel film formation and its role in soot generation. In the experiments, a skip-fired engine control strategy was applied to mimic the transient nature of engine cold-start operation. The results indicate that, under the same total number of injections, increasing the average in-cylinder equivalence ratio through longer injection durations improves combustion stability, as indicated by the decrease of the coefficient of variation of IMEP n (Net Indicated Mean Effective Pressure) from 6.0 % to 0.6 %. However, this strategy leads to higher soot emissions, which increased by nearly an order of magnitude, primarily due to enhanced wall-film formation. In contrast, increasing the number of injections while maintaining a constant equivalence ratio significantly impacts fuel-film deposition and, consequently, soot emissions, with a fivefold reduction of the measured engine-out soot, decreasing from 3.5 mg to 0.7 mg. A soot-film correlation was developed and achieved a high coefficient of determination (r 2 = 0.95) and was further extended to account for spark timing effects. These findings confirm the effectiveness of split-injection for avoiding wall film formation and soot emissions, and the critical role of fuel film in soot generation, supporting the hypothesis that pool fires play a crucial role in contributing to soot formation under these cold-start conditions. In conclusion, the study also indicates the value of a predictive soot-film correlation for developing cold-start emission control strategies.

Engine Cold-start↗

Studying Turbulence Using Numerical Simulation Databases: Proceedings of the Summer Program - No. 7

The Seventh Summer Program of the Center for Turbulence Research took place in the four-week period, July 5 to July 31, 1998. This was the largest CTR Summer Program to date, involving thirty-six participants from the U. S. and nine other countries. Thirty-one Stanford and NASA-Ames staff members facilitated and contributed to most of the Summer projects. A new feature, and perhaps a preview of the future programs, was that many of the projects were executed on non-NASA computers. These included supercomputers located in Europe as well as those operated by the Departments of Defense and Energy in the United States. In addition, several simulation programs developed by the visiting participants at their home institutions were used. Another new feature was the prevalence of lap-top personal computers which were used by several participants to carry out some of the work that in the past were performed on desk-top workstations. We expect these trends to continue as computing power is enhanced and as more researchers (many of whom CTR alumni) use numerical simulations to study turbulent flows. CTR's main role continues to be in providing a forum for the study of turbulence for engineering analysis and in facilitating intellectual exchange among the leading researchers in the field. Once again the combustion group was the largest. Turbulent combustion has enjoyed remarkable progress in using simulations to address increasingly complex and practically more relevant questions. The combustion group's studies included such challenging topics as fuel evaporation, soot chemistry, and thermonuclear reactions. The latter study was one of three projects related to the Department of Energy's ASCI Program (www.llnl.gov/asci); the other two (rocket propulsion and fire safety) were carried out in the turbulence modeling group. The flow control and acoustics group demonstrated a successful application of the so-called evolution algorithms which actually led to a previously unknown forcing strategy for jets yielding increased spreading rate. A very efficient algorithm for flow in complex geometries with moving boundaries based on the immersed boundary forcing technique was tested with very encouraging results. Also a new strategy for the destruction of aircraft trailing vortices was introduced and tested. The Reynolds Averaged Modeling (RANS) group demonstrated that the elliptic relaxation concept for RANS calculations is also applicable to transonic flows with shocks; however, prediction of laminar/turbulent transition remains an important pacing item. A large fraction of the LES effort was devoted to the development and testing of a new algorithmic procedure (as opposed to phenomenological model) for subgrid scale modeling based on regularized de-filtering of the flow variables. This appears to be a very promising approach, and a significant effort is currently underway to assess its robustness in high Reynolds number flows and in conjunction with numerical methods for complex flows. As part of the Summer Program two review tutorials were given on Turbulent structures in hydrocarbon pool fires (Sheldon Tieszen), and Turbulent combustion modeling: from RANS to LES via DNS (Luc Vervisch); and two seminars entitled Assessment of turbulence models for engineering applications (Paul Durbin) and Subgrid-scale modeling for non-premixed, turbulent reacting flows (James Riley) were presented. A number of colleagues from universities, government agencies, and industry attended the final presentations of the participants on July 31 and participated in the discussions. There are twenty-six papers in this volume grouped in five areas. Each group is preceded with an overview by its coordinator.

Source record↗

Spatiotemporal flame propagations, combustion and solid particle emissions from lean and stoichiometric gasoline direct injection engine operation

Increased particle number and mass emissions in gasoline direct injection (GDI) engines should require to investigate in-cylinder flame and combustion characteristics associated with primary source of particle emissions. In this article, in-cylinder spatiotemporal flame luminosity is quantitatively characterized to features combustion process and solid particle emissions from a GDI engine operating in two lean and one stoichiometric modes. Low- and high-steady state operating points were used to compare combustion strategies on flame development and emission characteristics. A fiber-optic sensor composed of eight measurement channels detected the flame front and the direction of the flame propagation in the combustion chamber. Solid particle emissions in the exhaust were measured using an engine exhaust particle sizer and a micro soot sensor. Results of the experiments showed that two lean combustion modes by injection strategies resulted in distinct combustion and flame development. Lean combustion modes generated high diffusion flame by burning stratified rich-mixture. Although the lean cases resulted in strong diffusion flames, the lean-homogeneous produced similar particle size distributions with the stoichiometric mode with high ash particles. Piston pool fires on the piston surface in the lean-stratified mode induced a large accumulation mode with high particle mass concentrations.

42 ENGINEERING↗

Modeling Ethanol-Blend Fuel Sprays under Direct-Injection Spark-Ignition Engine Conditions

Computational modeling of fuel-injection and fuel-impingement processes was carried out to provide insight into previous experimental results obtained from a direct-injection spark-ignition research engine operated with various ethanol and aromatic fuel blends. Seven model fuels were evaluated using both single- and split-injection strategies. Model results showed that droplet vaporization enriched the concentrations of aromatic species in the liquid phase, especially those with low vapor pressure, and ethanol-containing fuels altered droplet sizes and led to increased liquid-phase mass in the cylinder. For the spray-modeling conditions studied, most of the injected fuel remained in the liquid phase, which would be expected to result in more fuel impingement on cylinder or piston surfaces. Fuel impinging on lower cylinder-temperature surfaces was found to create wall films that could interact with the piston during the compression stroke. Fuel impinging on hotter piston-temperature surfaces vaporized more readily, but for fuels with high ethanol and aromatic content, wall films remained and were highly concentrated with lower vapor pressure and aromatic compounds. These films could lead to pool fires during combustion and therefore significant particulate matter emissions.

BIOMASS FUELS,INORGANIC, ORGANIC, PHYSICAL, AND AN↗

Black carbon aerosol number and mass concentration measurements by picosecond short-range elastic backscatter lidar

Abstract Black carbon aerosol emissions are recognized as contributors to global warming and air pollution. There remains, however, a lack of techniques to remotely measure black carbon aerosol particles with high range and time resolution. This article presents a direct and contact-free remote technique to estimate the black carbon aerosol number and mass concentration at a few meters from the emission source. This is done using the Colibri instrument based on a novel technique, referred to here as Picosecond Short-Range Elastic Backscatter Lidar (PSR-EBL). To address the complexity of retrieving lidar products at short measurement ranges, we apply a forward inversion method featuring radiometric lidar calibration. Our method is based on an extension of a well-established light-scattering model, the Rayleigh–Debye–Gans for Fractal-Aggregates (RDG-FA) theory, which computes an analytical expression of lidar parameters. These parameters are the backscattering cross-sections and the lidar ratio for black carbon fractal aggregates. Using a small-scale Jet A-1 kerosene pool fire, we demonstrate the ability of the technique to quantify the aerosol number and mass concentration with centimetre range-resolution and millisecond time-resolution.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Editorial: Particulate Matter Emissions From Conventional and Reformulated Fuel Combustion: Advances in Experiments and Simulations

Particulate matter (PM or soot) emissions generated by combustion of conventional and reformulated fuels adversely impact the environment and human health (Bond et al., 2013; Landrigan et al., 2018; World Health Organization (WHO), 2016), generating public awareness and motivating efforts towards the mitigation of their harmful effects. Soot formation is one of the most complex phenomena in combustion, involving interactions between combustion chemistry, fluid mechanics, mass/heat transport, and particle dynamics, spanning different spatial and temporal scales. A fundamental understanding of soot formation process is thus necessary to achieve a strong reduction of PM emissions and design cleaner and more efficient combustion systems. This has motivated a longstanding and ongoing research activity aimed at improving our understanding of the physical and chemical processes involved in soot formation, well reviewed in these recent works (Wang and Chung, 2019; Michelsen et al., 2020; Martin et al., 2022). Despite the wide interest, the transition from gas-phase molecules to incipient soot particles is still elusive and the successive particle growth and oxidation processes are far from being fully understood, especially in conditions relevant to real-world applications. Recent advances in combustion PM emission diagnostic and computational capabilities helped in improving the predictability of fundamental chemical and aerosol models for practical applications, thus tackling some of the above-mentioned challenges. The aim of this research topic is to display the ongoing research efforts in addressing the existing gaps on particulate formation from various fuel sources and in conditions typical of practical combustion applications (e.g. flames, engines, pool fires), through both experimental and numerical approaches.

42 ENGINEERING↗

Evaluation of micron size carbon fibers released from burning graphite composites

Quantitative estimates were developed of micron carbon fibers released during the burning of graphite composites. Evidence was found of fibrillated particles which were the predominant source of the micron fiber data obtained from large pool fire tests. The fibrillation phenomena were attributed to fiber oxidation effects caused by the fire environment. Analysis of propane burn test records indicated that wind sources can cause considerable carbon fiber oxidation. Criteria estimates were determined for the number of micron carbon fibers released during an aircraft accident. An extreme case analysis indicated that the upper limit of the micron carbon fiber concentration level was only about half the permissible asbestos ceiling concentration level.

Sussholz, B.↗

The NASA Microgravity Combustion Space Experiment Program

This paper summarizes the scientific objectives of the expanded microgravity combustion science program including efforts in laminar and turbulent premixed-gas flames, laminar and turbulent gas-jet diffusion flames, droplet combustion, smoldering, flame spreading, pool fires, and metals combustion. Secondly, the paper will summarize the flight hardware that is currently flying and new hardware being proposed to carry out this extensive program of microgravity combustion experiments.

Sacksteder, Kurt↗

Boulder County Disasters: Mapping Forest Carbon Stocks to Understand Carbon Implications of Treatment and Wildfire

In recent years, record-breaking wildfire activities in the western US illustrate the need for fire mitigation efforts, such as forest fuels reduction treatments. Forests serve as crucial carbon sinks that combat the increasing effects of climate change while fuels reduction treatments may remove carbon from forested systems. As a result, forest managers need to find a balance between fire mitigation and carbon preservation. This project partnered with Boulder County Parks and Open Space (BCPOS) and the University of Colorado, Denver to investigate the 2020 Cal-Wood fire in Boulder County, Colorado. Using remote sensing data from Landsat 8 OLI, SRTM, Sentinel-2 MSI, and LiDAR, we mapped post-fire forest carbon pools and compared these values with values derived from measurements from plots on the ground. Results indicate that the correlations are R2=0.76 for aboveground live carbon, R2=0.44 for standing carbon, and R2=0.43 for aboveground dead carbon (R2=0.43), and R2=0.35 for total carbon (R2=0.35). Additionally, 38.5% of total carbon was stored in dead carbon and 14.4% was stored in live carbon. We next compared the post-fire pools between treated and untreated areas. Our analysis suggests fuels reduction treatments did not reduce carbon loss in the presence of wildfire enough to clearly distinguish the post-fire carbon in the treated and untreated areas. However, our final carbon maps still provide BCPOS and researchers with an opportunity to explore carbon estimation models based on remotely sensed data as well as a framework to evaluate fuels reduction treatment effectiveness and impact on forest carbon stocks for future wildfire events.

Sarah Hettema↗

Comparative genomics of pyrophilous fungi reveals a link between fire events and developmental genes

Forest fires generate a large amount of carbon that remains resident on the site as dead and partially ‘pyrolysed’ (i.e. burnt) material that has long residency times and constitutes a significant pool in fire–prone ecosystems. In addition, fire–induced hydrophobic soil layers, caused by condensation of pyrolysed waxes and lipids, increase post–fire erosion and can lead to long–term productivity losses. A small set of pyrophilous fungi dominate post–fire soils and are likely to be involved with the degradation of all these compounds, yet almost nothing is currently known about what these fungi do or the metabolic processes they employ. In this study, we sequenced and analyzed genomes from fungi isolated after Rim fire near Yosemite National Park in 2013 and showed the enrichment/expansion of CAZymes and families known to be involved in fruiting body initiation when compared to other basidiomycete fungi. We found gene families potentially involved in the degradation of the hydrophobic layer and pyrolysed organic matter, such as hydrophobic surface binding proteins, laccases (AA1_1), xylanases (GH10, GH11), fatty acid desaturases and tannases. Furthermore, pyrophilous fungi are important actors to restate the soil's functional capabilities.

59 BASIC BIOLOGICAL SCIENCES↗

Mapping Forest Carbon Stocks to Understand Carbon Implications of Treatment on Wildfire for the Calwood Fire, Boulder County Colorado

Recent, record-breaking wildfire activity in the western U.S. illustrates the need for fire mitigation, such as forest fuels reduction treatments. Forests serve as crucial carbon sinks that combat the increasing effects of climate change, but fuels reduction treatments may remove carbon from forested systems. As a result, forest managers need to find a balance between fire mitigation and carbon preservation. This project partnered with Boulder County Parks and Open Space (BCPOS) and the University of Colorado, Denver to investigate the 2020 Cal-Wood fire in Boulder County, Colorado. Using remote sensing data from Landsat 8 Operational Land Imager, Shuttle Radar Topography Mission, Sentinel-2 MultiSpectral Imagery, and LiDAR, we mapped post-fire forest carbon pools and compared these values with values derived from field measurements. The analysis suggested that fuels reduction treatments did not reduce carbon loss in the presence of wildfire enough to clearly distinguish post-fire carbon between treated and untreated areas. However, the final carbon maps provide BCPOS and researchers with an opportunity to explore carbon estimation models based on remote sensing data as well as a framework to evaluate fuels reduction treatment effectiveness and impact on forest carbon stocks for future wildfire events.

Sarah Hettema↗