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Simulation model for plant growth in controlled environment systems

The role of the mathematical model is to relate the individual processes to environmental conditions and the behavior of the whole plant. Using the controlled-environment facilities of the phytotron at North Carolina State University for experimentation at the whole-plant level and methods for handling complex models, researchers developed a plant growth model to describe the relationships between hierarchial levels of the crop production system. The fundamental processes that are considered are: (1) interception of photosynthetically active radiation by leaves, (2) absorption of photosynthetically active radiation, (3) photosynthetic transformation of absorbed radiation into chemical energy of carbon bonding in solube carbohydrates in the leaves, (4) translocation between carbohydrate pools in leaves, stems, and roots, (5) flow of energy from carbohydrate pools for respiration, (6) flow from carbohydrate pools for growth, and (7) aging of tissues. These processes are described at the level of organ structure and of elementary function processes. The driving variables of incident photosynthetically active radiation and ambient temperature as inputs pertain to characterization at the whole-plant level. The output of the model is accumulated dry matter partitioned among leaves, stems, and roots; thus, the elementary processes clearly operate under the constraints of the plant structure which is itself the output of the model.

Raper, C. D., Jr.

Selection of Cultivars for use in Controlled Environment Life Support System (CELSS) Human Rated Test Facility (HRTF) Trials

The aims under this training grant, as under the subsequent fellowship, were to elaborate the theory and technique of cultivar evaluation for specialized controlled environments, then to employ the technique on selected crops, ultimately conducting cultivar trials, and making the knowledge gained available for use in NASA's space program. We undertook a comprehensive search of the Cornell agricultural library (Mann library) and its data-bases for any and all material relating to cultivar evaluation of vegetable crops, and also developed the logic of how to go about narrowing down the field of contending cultivars when undertaking cultivar trials. The results of this work, the principal outcome of the grant, are reflected in his MS thesis, particularly in Chapter 2, "Commercial and Scientific Literature," and even more so in Chapter 8, "Selecting cultivars and lines for screening." David also attended annual conferences of vegetable crop plant breeders, annual yield trials and breeding trials for vegetable crops, as well as relevant professional conferences such as the ASHS annual meetings, and the others.

Langhans, Robert W.

Gaseous emissions from plants in controlled environments

Plant growth in a controlled ecological life support system may entail the build-up over extended time periods of phytotoxic concentrations of volatile organic compounds produced by the plants themselves. Ethylene is a prominent gaseous emission of plants, and is the focus of this report. The objective was to determine the rate of ethylene release by spring wheat, white potato, and lettuce during early, middle, and late growth stages, and during both the light and dark segments of the diurnal cycle. Plants grown hydroponically using the nutrient film technique were covered with plexiglass containers for 4 to 6 h. At intervals after enclosure, gas samples were withdrawn with a syringe and analyzed for ethylene with a gas chromatograph. Lettuce produced 10 to 100 times more ethylene than wheat or potato, with production rates ranging from 141 to 158 ng g-dry/wt/h. Wheat produced from 1.7 to 14.3 ng g-dry/wt/h, with senescent wheat producing the least amount and flowering wheat the most. Potatoes produced the least amount of ethylene, with values never exceeding 5 ng g-dry/wt/h. Lettuce and potatoes each produced ethylene at similar rates whether in dark period or light period. Ethylene sequestering of 33 to 43 percent by the plexiglass enclosures indicated that these production estimates may be low by one-third to one-half. These results suggest that concern for ethylene build-up in a contained atmosphere should be greatest when growing lettuce, and less when growing wheat or potato.

Dubay, Denis T.

Light-Emitting Diodes (LED) for Primary Animal Habitat Lighting in Highly Controlled Environments

Significant alterations in Biological Clock responses have been reported following sidereal time changes (e.g., Jet-lag), and exposure to microgravity (e.g., daytime sleepiness). Additionally, light reduces circulating melatonin (spectral specificity greatest between 450-500 nm). It was hypothesized that LEDs can replace the current light sources used in zero gravity and terrestrial research laboratories because of their small size, low mass, low energy consumption and long functional life. This report evaluates the capacity of LEDs to entrain the circadian system of rats as judged by measurement of overt behavioral circadian rhythms (activity, feeding, drinking). These data were collected in highly controlled environments similar to the shuttle Animal Enclosure Modules. Two groups were compared: control - animals exposed to standard cool-white fluorescent lights, and test - animals exposed to LEDs with a spectral power distribution matching the fluorescent lights. Gross locomotor activity, feeding and drinking frequencies were continuously monitored and stored at 10 minute intervals. Animals were exposed to the following photoperiods: 28 days of 12L:12D, 19 days of 24L:0D and 16 days of 12L:12D. Light intensities tested varied between 0.1 to 100 lux. Rats received food and water ad libitum, and temperature and humidity were controlled throughout the study. The general health status of all rats was acceptable for each day of this study. No incidents of aggressive behavior were observed. Growth, locomotor activity, food and water consumption were comparable for all groups of animals, i.e, the circadian characteristics of the animals under these conditions were comparable. These results indicate that LED arrays are as effective in maintaining circadian rhythm stability as the commonly used cool-white fluorescent light sources. LEDs with their flexible spectrum, low energy requirements and minimal heat production have advantages for some chronopharmacology studies and for microgravity animal habitats.

Winget, C. M.

Phytochrome-mediated responses: Implications for controlled environment research facilities

Light is undoubtedly the most important environmental variable for plant growth and development; plants not only use radiant energy in photosynthesis, they also respond to the quantity, quality, direction and timing of incident radiation through photomorphogenic response that can have huge effects on the rate of growth and the pattern of development. It is surprising, therefore, that the manufacturers and suppliers of controlled environment facilities have been singularly uninventive in the design of the lighting assemblies they provide. The consumer has one choice only - a lighting assembly that provides irradiance levels usually only a fraction of sunlight, and a control system that is limited to regulating the timing of the on-off switch. The reasons for these limitations are partly technological, but in the main they result from ignorance on the part of both the consumer and the manufacturer. A specific and powerful example of this ignorance relates to the importance of the so-called far-red wavelengths (FR = 700-800 nm). Because the human eye can hardly detect wavelengths above 700 nm, and photosynthesis also cuts off at about 700 nm, the majority of plant and crop physiologists are still almost completely unaware that FR radiation can have massive effects on growth rate and development. In consequence, most growth cabinets have light sources based on fluorescent tubes, and provide very little FR apart from that emitted by a token number of small incandescent bulbs. Larger growth facilities often use broader spectrum light sources, but growth facilities that provide the capability to vary the FR incident upon the plants are about as abundant as seals in the Sahara. This article sets the background of the significance of FR radiation in the natural environment and its importance for plant growth and development in the hope that it might inform intelligently those concerned with improving the design of plant growth facilities.

Smith, Harry

NASA's Controlled Environment Agriculture Testing for Space Habitats

NASA and other space agencies have an interest in using plants for human life support in space. The plants could provide food and O2 for the humans, while removing CO2 and helping purify wastewater. Studies to date have shown that a wide range of crops can be grown in controlled environment conditions envisioned for space. Light is a critical factor both for crop productivity and system power costs, and recent improvements in LEDs make them a preferred lighting option for space. Because space systems would be tightly closed, issues such as ethylene build-up and management must be considered. Ultimately, the costs and reliability of biological life support options must be compared with more conventional life support approaches. Findings to date suggest that about 20-25 sq. meters of crops could supply the O2 for one human, while about 50 sq. meters would be required for food (dietary calories).

Bioregenerative

Studies on maximum yield of wheat for the controlled environments of space

The economic feasibility of using food-producing crop plants in a closed ecological Life-Support System (CELSS) will ultimately depend on the energy and area (or volume) required to provide the nutritional requirements for each person. Energy and area requirements are, to some extent, inversely related; that is, an increased energy input results in a decreased area requirement and vice versa. A major goal of the research effort was to determine the controlled-environment good-production efficiency of wheat per unit area, per unit time, and per unit energy input.

Bugbee, B. G.

Current and potential productivity of wheat for a controlled environment life support system

Several determinants of crop growth are analyzed to determine theoretical and potentially achievable productivity. These include: incident photosynthetic photon flux (PPF); percent absorption of the incident PPF by photo synthetic tissue; photosynthetic efficiency; respiratory carbon use efficiency; and harvest index. The effects of optimal environmental and cultural factors on each of these determinants are also investigated. Results indicate that an increase in the percentage of absorbed photons is responsible for most of the improvement in wheat yields in an optimal controlled environment. An integrated PPF of 150 mol/sq m per d has produced 60 g/sq m per d of grain. There is almost a linear increase in wheat yields with increasing PPF. At intermediate and equal integrated daily PPF levels, photoperiod had little effect on yield per day or energy efficiency. Decreasing temperature from 23 to 17 deg increased yield per day by 20 percent but increased the life cycle from 62 to 89 days.

Bugbee, B. G.

Ultrastable environment control for the NEID spectrometer: design and performance demonstration

Two key areas of emphasis in contemporary experimental exoplanet science are the detailed characterization of transiting terrestrial planets and the search for Earth analog planets to be targeted by future imaging missions. Both of these pursuits are dependent on an order-of-magnitude improvement in the measurement of stellar radial velocities (RV), setting a requirement on single-measurement instrumental uncertainty of order 10 cm∕s. Achieving such extraordinary precision on a high-resolution spectrometer requires thermomechanically stabilizing the instrument to unprecedented levels. We describe the environment control system (ECS) of the NEID spectrometer, which will be commissioned on the 3.5-m WIYN Telescope at Kitt Peak National Observatory in 2019, and has a performance specification of on-sky RV precision <50 cm/s. Because NEID’s optical table and mounts are made from aluminum, which has a high coefficient of thermal expansion, sub-milliKelvin temperature control is especially critical. NEID inherits its ECS from that of the Habitable-Zone Planet Finder (HPF), but with modifications for improved performance and operation near room temperature. Our full-system stability test shows the NEID system exceeds the already impressive performance of HPF, maintaining vacuum pressures below 10(exp −6) Torr and a root mean square (RMS) temperature stability better than 0.4 mK over 30 days. Our ECS design is fully open-source; the design of our temperature-controlled vacuum chamber has already been made public, and here we release the electrical schematics for our custom temperature monitoring and control system.

Paul Robertson

Simulation studies of STOL airplane operations in metropolitan downtown and airport air traffic control environments

The operating problems and equipment requirements for STOL airplanes in terminal area operations in simulated air traffic control (ATC) environments were studied. These studies consisted of Instrument Flight Rules (IFR) arrivals and departures in the New York area to and from a downtown STOL port, STOL runways at John F. Kennedy International Airport, or STOL runways at a hypothetical international airport. The studies were accomplished in real time by using a STOL airplane flight simulator. An experimental powered lift STOL airplane and two in-service airplanes having high aerodynamic lift (i.e., STOL) capability were used in the simulations.

Sawyer, R. H.

A system and methodology for measuring volatile organic compounds produced by hydroponic lettuce in a controlled environment

A system and methodology were developed for the nondestructive qualitative and quantitative analysis of volatile emissions from hydroponically grown 'Waldmann's Green' leaf lettuce (Lactuca sativa L.). Photosynthetic photon flux (PPF), photoperiod, and temperature were automatically controlled and monitored in a growth chamber modified for the collection of plant volatiles. The lipoxygenase pathway products (Z)-3-hexenal, (Z)-3-hexenol, and (Z)-3-hexenyl acetate were emitted by lettuce plants after the transition from the light period to the dark period. The volatile collection system developed in this study enabled measurements of volatiles emitted by intact plants, from planting to harvest, under controlled environmental conditions.

NASA Discipline Life Support Systems

Biomass accumulation in hydroponically grown sweetpotato in a controlled environment: a preliminary study

In the development of a plant growth model, the assumptions made and the general equations representing an understanding of plant growth are gradually refined as more information is acquired through experimentation. One such experiment that contributed to sweetpotato model development consisted of measuring biomass accumulation of sweetpotato grown in hydroponic culture in a plant growth chamber. Plants were started from fifteen centimeter long 'TU-82-155' sweetpotato vine cuttings spaced 25 cm apart in each of 18 rectangular growing channels (0.15 by 0.15 by 1.2m) in a system designed to use the nutrient film technique (NFT). Each channel contained four plants. The 3.5m by 5.2m plant growth chamber environmental parameters included an 18h photoperiod, 500 micromoles m-2 s-1 of photosynthetic photon flux (PPF), and a diurnal light/dark temperature of 28 degrees C/22 degrees C. The relative humidity was 80 +/- 5% and the CO2 partial pressure was ambient (350 ppm). The nutrient solution contained in 30L reservoirs was a modified half Hoagland's solution with a 1:2.4 N:K ratio and a pH of 6.2. Solution replenishment occurred when the electrical conductivity (EC) level dropped below 1050. Plants were harvested at 15 days after planting (DAP) and weekly thereafter until day 134. By 57 DAP, stems and fibrous roots had acquired 90% of their total dry biomass, while leaves had reached 84% of their maximum dry biomass. Beginning at 64 DAP dry biomass accumulation in the storage roots dominated the increase in dry biomass for the plants. Dry weight of storage roots at 120 DAP was 165 g/plant or 1.1 kg/m2. Resulting growth curves were consistent with the physiological processes occurring in the plant. Results from this study will be incorporated in a plant growth model for use in conjunction with controlled life support systems for long-term manned space missions.

Biomass

Sole-Source Lighting for Controlled-Environment Agriculture

Since plants on Earth evolved under broad-spectrum solar radiation, anytime they are grown exclusively under electric lighting that does not contain all wavelengths in similar proportion to those in sunlight, plant appearance and size could be uniquely different. Nevertheless, plants have been grown for decades under fluorescent (FL) (1) + incandescent (IN) (2) lamps as a sole source of lighting (SSL), and researchers have become comfortable that, in certain proportions of FL + IN for a given species, plants can appear "normal" relative to their growth outdoors. The problem with using such traditional SSLs for commercial production typically is short lamp lifespans and not obtaining enough photosynthetically active radiation (PAR, 400-700 nm) when desired. These limitations led to supplementation of FL + IN lamp outputs with longer-lived, high-intensity discharge (HID) lamps in growth chambers (3). As researchers became comfortable that mixes of orange-biased high-pressure sodium (HPS) and blue-biased metal halide (MH) HIDs together also could give normal plant growth at higher intensities, growth chambers and phytotrons subsequently were equipped mainly with HID lamps, with their intense thermal output filtered out by ventilated light caps or thermal-controlled water barriers. For the most part, IN and HID lamps have found a home in commercial protected horticulture, usually for night-break photoperiod lighting (IN) or for seasonal supplemental lighting (mostly HPS) in greenhouses. However, lack of economically viable options for SSL have held back aspects of year-round indoor agriculture from taking off commercially.

Controlled-Environment Agriculture

Implementation of a three degree of freedom, motor/brake hybrid force output device for virtual environment control tasks

The advent of high resolution, physical model based computer graphics has left a gap in the design of input/output technology appropriate for interacting with such complex virtual world models. Since virtual worlds consist of physical models, it is appropriate to output the inherent force information necessary for the simulation to the user. The detailed design, construction, and control of a three degree freedom force output joystick will be presented. A novel kinematic design allows all three axes to be uncoupled, so that the system inertia matrix is diagonal. The two planar axes are actuated through an offset gimbal, and the third through a sleeved cable. To compensate for friction and inertia effects, this transmission is controlled by a force feedforward and a closed force feedback proportional loop. Workspace volume is a cone of 512 cubic inches, and the device bandwidth is maximized at 60 Hz for the two planar and 30 Hz for the third axis. Each axis is controlled by a motor/proportional magnetic particle brake combination fixed to the base. The innovative use of motors and brakes allows objects with high resistive torque requirements to be simulated without the stability and related safety issues involved with high torque, energy storing motors alone. Position, velocity, and applied endpoint force are sensed directly. Different control strategies are discussed and implemented, with an emphasis on how virtual environment force information, generated by the MIT Media Lab Computer Graphics and Animation Group BOLIO system, is transmitted to the device controller. The design constraints for a kinesthetic force feedback device can be summarized as: How can the symbiosis between the sense of presence in the virtual environment be maximized without compromising the interaction task under the constraints of the mechanical device limitations? Research in this field will yield insights to the optimal human sensory feedback mix for a wide spectrum of control and interaction problems. A flexible research tool that is designed as an easily reproducible product prototype has been constructed to explore the variety of possible force interaction.

Russo, Massimo

Piloted simulation of an air-ground profile negotiation process in a time-based Air Traffic Control environment

Historically, development of airborne flight management systems (FMS) and ground-based air traffic control (ATC) systems has tended to focus on different objectives with little consideration for operational integration. A joint program, between NASA's Ames Research Center (Ames) and Langley Research Center (Langley), is underway to investigate the issues of, and develop systems for, the integration of ATC and airborne automation systems. A simulation study was conducted to evaluate a profile negotiation process (PNP) between the Center/TRACON Automation System (CTAS) and an aircraft equipped with a four-dimensional flight management system (4D FMS). Prototype procedures were developed to support the functional implementation of this process. The PNP was designed to provide an arrival trajectory solution which satisfies the separation requirements of ATC while remaining as close as possible to the aircraft's preferred trajectory. Results from the experiment indicate the potential for successful incorporation of aircraft-preferred arrival trajectories in the CTAS automation environment. Fuel savings on the order of 2 percent to 8 percent, compared to fuel required for the baseline CTAS arrival speed strategy, were achieved in the test scenarios. The data link procedures and clearances developed for this experiment, while providing the necessary functionality, were found to be operationally unacceptable to the pilots. In particular, additional pilot control and understanding of the proposed aircraft-preferred trajectory, and a simplified clearance procedure were cited as necessary for operational implementation of the concept.

Williams, David H.