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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 433 records · Page 24
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

High-quality RNA extraction and the regulation of genes encoding cellulosomes are correlated with growth stage in anaerobic fungi

Anaerobic fungi produce biomass-degrading enzymes and natural products that are important to harness for several biotechnology applications. Although progress has been made in the development of methods for extracting nucleic acids for genomic and transcriptomic sequencing of these fungi, most studies are limited in that they do not sample multiple fungal growth phases in batch culture. In this study, we establish a method to harvest RNA from fungal monocultures and fungal–methanogen co-cultures, and also determine an optimal time frame for high-quality RNA extraction from anaerobic fungi. Based on RNA quality and quantity targets, the optimal time frame in which to harvest anaerobic fungal monocultures and fungal-methanogen co-cultures for RNA extraction was 2-5 days of growth post-inoculation. When grown on cellulose, the fungal strain Anaeromyces robustus cocultivated with the methanogen Methanobacterium bryantii upregulated genes encoding fungal carbohydrate-active enzymes and other cellulosome components relative to fungal monocultures during this time frame, but expression patterns changed at 24-hour intervals throughout the fungal growth phase. These results demonstrate the importance of establishing methods to extract high-quality RNA from anaerobic fungi at multiple time points during batch cultivation.

Brown, Jennifer L.↗
DOE OSTI2023

SARS-CoV-2 raw wastewater surveillance from student residences on an urban university campus

The COVID-19 pandemic brought about an urgent need to monitor the community prevalence of infection and detect the presence of SARS-CoV-2. Testing individual people is the most reliable method to measure the spread of the virus in any given community, but it is also the most expensive and time-consuming. Wastewater-based epidemiology (WBE) has been used since the 1960s when scientists implemented monitoring to measure the effectiveness of the Polio vaccine. Since then, WBE has been used to monitor populations for various pathogens, drugs, and pollutants. In August 2020, the University of Tennessee-Knoxville implemented a SARS-CoV-2 surveillance program that began with raw wastewater surveillance of the student residence buildings on campus, the results of which were shared with another lab group on campus that oversaw the pooled saliva testing of students. Sample collection began at 8 am, and the final RT-qPCR results were obtained by midnight. The previous day’s results were presented to the campus administrators and the Student Health Center at 8 am the following morning. The buildings surveyed included all campus dormitories, fraternities, and sororities, 46 buildings in all representing an on-campus community of over 8,000 students. The WBE surveillance relied upon early morning “grab” samples and 24-h composite sampling. Because we only had three Hach AS950 Portable Peristaltic Sampler units, we reserved 24-h composite sampling for the dormitories with the highest population of students. Samples were pasteurized, and heavy sediment was centrifuged and filtered out, followed by a virus concentration step before RNA extraction. Each sample was tested by RT-qPCR for the presence of SARS-CoV-2, using the CDC primers for N Capsid targets N1 and N3. The subsequent pooled saliva tests from sections of each building allowed lower costs and minimized the total number of individual verification tests that needed to be analyzed by the Student Health Center. Our WBE results matched the trend of the on-campus cases reported by the student health center. The highest concentration of genomic copies detected in one sample was 5.06 × 10 7 copies/L. Raw wastewater-based epidemiology is an efficient, economical, fast, and non-invasive method to monitor a large community for a single pathogen or multiple pathogen targets.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

Decay of enveloped SARS-CoV-2 and non-enveloped PMMoV RNA in raw sewage from university dormitories

Although severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA has been frequently detected in sewage from many university dormitories to inform public health decisions during the COVID-19 pandemic, a clear understanding of SARS-CoV-2 RNA persistence in site-specific raw sewage is still lacking. To investigate the SARS-CoV-2 RNA persistence, a field trial was conducted in the University of Tennessee dormitories raw sewage, similar to municipal wastewater. The decay of enveloped SARS-CoV-2 RNA and non-enveloped Pepper mild mottle virus (PMMoV) RNA was investigated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in raw sewage at 4°C and 20°C. Temperature, followed by the concentration level of SARS-CoV-2 RNA, was the most significant factors that influenced the first-order decay rate constants (k) of SARS-CoV-2 RNA. The mean k values of SARS-CoV-2 RNA were 0.094 day –1 at 4°C and 0.261 day –1 at 20°C. At high-, medium-, and low-concentration levels of SARS-CoV-2 RNA, the mean k values were 0.367, 0.169, and 0.091 day –1 , respectively. Furthermore, there was a statistical difference between the decay of enveloped SARS-CoV-2 and non-enveloped PMMoV RNA at different temperature conditions. The first decay rates for both temperatures were statistically comparable for SARS-CoV-2 RNA, which showed sensitivity to elevated temperatures but not for PMMoV RNA. This study provides evidence for the persistence of viral RNA in site-specific raw sewage at different temperature conditions and concentration levels.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

COVID-19 trends at the University of Tennessee: predictive insights from raw sewage SARS-CoV-2 detection and evaluation and PMMoV as an indicator for human waste

Wastewater-based epidemiology (WBE) has become a valuable tool for monitoring the prevalence of SARS-CoV-2 on university campuses. However, concerns about effectiveness of raw sewage as a COVID-19 early warning system still exist, and it’s not clear how useful normalization by simultaneous comparison of Pepper Mild Mottle Virus (PMMoV) is in addressing variations resulting from fecal discharge dilution. This study aims to contribute insights into these aspects by conducting an academic-year field trial at the student residences on the University of Tennessee, Knoxville campus, raw sewage. This was done to investigate the correlations between SARS-CoV-2 RNA load, both with and without PMMoV normalization, and various parameters, including active COVID-19 cases, self-isolations, and their combination among all student residents. Significant positive correlations between SARS-CoV-2 RNA load a week prior, during the monitoring week, and the subsequent week with active cases. Despite these correlations, normalization by PMMoV does not enhance these associations. These findings suggest the potential utility of SARS-CoV-2 RNA load as an early warning indicator and provide valuable insights into the application and limitations of WBE for COVID-19 surveillance specifically within the context of raw sewage on university campuses.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2025

Fabrication and Evaluation of Large Alumina Crucibles by Vat Photopolymerization Additive Manufacturing for High-Temperature Actinide Chemistry

Additive manufacturing (AM) offers opportunities to advance the design and function of ceramic tooling in high temperature actinide pyrochemistry. In technical ceramics such as alumina, conventional forming techniques often restrict design flexibility and can limit experimental progress. In this study, we investigate the use of vat photopolymerization (VP) with commercial resins to fabricate large-scale alumina crucibles, reaching dimensions up to 125 mm, which is significantly larger than typically reported for dense VP ceramics. Notably, these additively manufactured components are produced using consumer-grade hardware, which limits process control, but offers significant upside in scalability and accessibility. Using microscopy and X-ray computed tomography, the VP alumina parts have high bulk densities above 95%, but also the prevalence of AM-induced artifacts and surface defects. Mechanical testing showed these defects to significantly reduce flexural strength and compromise part reliability. Electrorefining trials under sustained exposure to molten salts and metals reveal mixed results, with the AM material exhibiting high chemical compatibility, but mechanical failures due to the reduced strength were prevalent. Our findings illustrate both the promise and current limitations of AM ceramics for actinide chemistry, and point toward future improvements in process optimization, design strategies, and part screening to enhance performance and reliability.

Materials science↗
DOE OSTI2025

Untargeted GC-MS Metabolic Profiling of Anaerobic Gut Fungi Reveals Putative Terpenoids and Strain-Specific Metabolites

Background/Objectives: Anaerobic gut fungi (Neocallimastigomycota) are biotechnologically relevant, lignocellulose-degrading microbes with under-explored biosynthetic potential for secondary metabolites. Untargeted metabolomic profiling with gas chromatography–mass spectrometry (GC-MS) was applied to two gut fungal strains, Anaeromyces robustus and Caecomyces churrovis, to establish a foundational metabolomic dataset to identify metabolites and provide insights into gut fungal metabolic capabilities. Methods: Gut fungi were cultured anaerobically in rumen-fluid-based media with a soluble substrate (cellobiose), and metabolites were extracted using the Metabolite, Protein, and Lipid Extraction (MPLEx) method, enabling metabolomic and proteomic analysis from the same cell samples. Samples were derivatized and analyzed via GC-MS, followed by compound identification by spectral matching to reference databases, molecular networking, and statistical analyses. Results: Distinct metabolites were identified between A. robustus and C. churrovis, including 2,3-dihydroxyisovaleric acid produced by A. robustus and maltotriitol, maltotriose, and melibiose produced by C. churrovis. C. churrovis may polymerize maltotriose to form an extracellular polysaccharide, like pullulan. GC-MS profiling potentially captured sufficiently volatile products of proteomically detected, putative non-ribosomal peptide synthetases and polyketide synthases of A. robustus and C. churrovis. The triterpene squalene and triterpenoid tetrahymanol were putatively identified in A. robustus and C. churrovis. Their conserved, predicted biosynthetic genes—squalene synthase and squalene tetrahymanol cyclase—were identified in A. robustus, C. churrovis, and other anaerobic gut fungal genera. Conclusions: This study provides a foundational, untargeted metabolomic dataset to unmask gut fungal metabolic pathways and biosynthetic potential and to prioritize future efforts for compound isolation and identification.

Biochemistry & Molecular Biology↗
DOE OSTI2021

The Gravity Collective: A Search for the Electromagnetic Counterpart to the Neutron Star–Black Hole Merger GW190814

We present optical follow-up imaging obtained with the Katzman Automatic Imaging Telescope, Las Cumbres Observatory Global Telescope Network, Nickel Telescope, Swope Telescope, and Thacher Telescope of the LIGO/Virgo gravitational wave (GW) signal from the neutron star–black hole (NSBH) merger GW190814. We searched the GW190814 localization region (19 deg 2 for the 90th percentile best localization), covering a total of 51 deg 2 and 94.6% of the two-dimensional localization region. Analyzing the properties of 189 transients that we consider as candidate counterparts to the NSBH merger, including their localizations, discovery times from merger, optical spectra, likely host galaxy redshifts, and photometric evolution, we conclude that none of these objects are likely to be associated with GW190814. Based on this finding, we consider the likely optical properties of an electromagnetic counterpart to GW190814, including possible kilonovae and short gamma-ray burst afterglows. Using the joint limits from our follow-up imaging, we conclude that a counterpart with an r-band decline rate of 0.68 mag day -1 , similar to the kilonova AT 2017gfo, could peak at an absolute magnitude of at most -17.8 mag (50% confidence). Our data are not constraining for "red" kilonovae and rule out "blue" kilonovae with M > 0.5 M ⊙ (30% confidence). We strongly rule out all known types of short gamma-ray burst afterglows with viewing angles <17° assuming an initial jet opening angle of ~5$^°_∙$2 and explosion energies and circumburst densities similar to afterglows explored in the literature. Lastly, we explore the possibility that GW190814 merged in the disk of an active galactic nucleus, of which we find four in the localization region, but we do not find any candidate counterparts among these sources.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2025

The Gravity Collective: A Comprehensive Analysis of the Electromagnetic Search for the Binary Neutron Star Merger GW190425

We present an ultraviolet to infrared search for the electromagnetic (EM) counterpart to GW190425, the second ever binary neutron star merger discovered by the LIGO-Virgo-KAGRA Collaboration. GW190425 was more distant and had a larger localization area than GW170817, so we use a new tool, Teglon, to redistribute the GW190425 localization probability in the context of galaxy catalogs within the final localization volume. We derive a 90th percentile area of 6688 deg 2 , a ∼1.5× improvement relative to the LIGO/Virgo map, and show how Teglon provides an order-of-magnitude boost to the search efficiency of small (≤1 deg 2 ) field-of-view instruments. We combine our data with a large, publicly reported imaging data set, covering 9078.59 deg 2 of unique area and 48.13% of the LIGO/Virgo-assigned localization probability, to calculate the most comprehensive kilonova (KN), short gamma-ray burst (sGRB) afterglow, and model-independent constraints on the EM emission from a hypothetical counterpart to GW190425 to date under the assumption that no counterpart was found in these data. If the counterpart were similar to AT 2017gfo, there would be a 28.4% chance of it being detected in the combined data set. We are relatively insensitive to an on-axis sGRB, and rule out a generic transient with a similar peak luminosity and decline rate as AT 2017gfo to 30% confidence. Finally, across our new imaging and publicly reported data, we find 28 candidate optical counterparts that we cannot rule out as being associated with GW190425, finding that four such counterparts discovered within the localization volume and within 5 days of merger exhibit luminosities consistent with a KN.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Microbial Taxa Distribution Data From 16S rRNA Analysis Of Desalination Operations At Carlsbad, CA And Tampa Bay, FL

This data set list the distribution of microbial taxa from three sets of sampling campaigns from unit operations in two large desalination facilities in the US conducted between March and May 2021. The desalination plants include the Claude "Bud" Lewis Carlsbad Desalination Plant in Carlsbad, California and the Seater Desalination facility in Tampa Bay, Florida.

16s rRNA gene analysis↗
DOE OSTI2022

Comparison of ablators for the polar direct drive exploding pusher platform

We examine the performance of pure boron, boron carbide, high density carbon, and boron nitride ablators in the polar direct drive exploding pusher (PDXP) platform. The platform uses the polar direct drive conguration at the National Ignition Facility to drive high ion temperatures in a room temperature capsule and has potential applications for plasma physics studies and as a neutron source. The higher tensile strength of these materials compared to plastic enables a thinner ablator to support higher gas pressures, which could help optimize its performance for plasma physics experiments, while ablators containing boron enable the possiblity of collecting addtional data to constrain models of the platform. Applying recently developed and experimentally validated equation of state models for the boron materials, we examine the performance of these materials as ablators in 2D simulations, with particular focus on changes to the ablator and gas areal density, as well as the predicted symmetry of the inherently 2D implosion.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2020

Safeguards Technical Objectives for Thorium Molten Salt Reactor Fuel Cycles

China, Denmark, France, India, Indonesia, the United Kingdom, and the United States, among others, are exploring commercial thorium fuel cycles for domestic power production and/or international export. For any thorium fuel cycle facilities built and/or operated in a non-nuclear weapon State with a comprehensive safeguards agreement, those facilities and any associated nuclear material would be subject to international nuclear safeguards by the International Atomic Energy Agency (IAEA). Thorium fuel cycle facilities offered for the application of safeguards under a voluntary offer agreement by a nuclear weapon State would also be subject to safeguards, if selected by the IAEA. Compared to uranium/plutonium fuel cycle facilities, thorium fuel cycle facilities are comparatively diverse from an inspection and accounting perspective, differing greatly in material type, form, and design from uranium/plutonium fuel cycle facilities and also from each other. Of these, the most complex thorium fuel cycles – and therefore likely the most challenging to the implementation of international nuclear safeguards – are those that include thorium molten salt reactors. With thorium fuel cycles approaching near-term commercial deployment timelines, it is imperative that the international nuclear safeguards community begins to think about how to adapt existing international nuclear safeguards concepts and approaches to thorium fuel cycles, as well as mitigate any gaps that may exist. This paper focuses on the development of recommended safeguards technical objectives for efficient and effective application of international nuclear safeguards measures (e.g., containment / surveillance, non-destructive assay, destructive assay, environmental sampling) to thorium molten salt reactors.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗
DOE OSTI2021

Design Considerations for the Field Use of International Nuclear Safeguards Technology

The process of developing and deploying safeguards technology should be carried out in such a way that it supports the International Atomic Energy Agency’s (IAEA’s) international safeguards verification mission, while also being efficient in terms of both cost and time. This process, however, may be hindered by a general lack of nuclear facility operating experience among the scientists or engineers designing safeguards technology, or the inability to interact with the IAEA inspector end user. As a result, equipment designers may have difficulty understanding inspector needs and the limitations of using safeguards technology in the field, often leading to inefficiencies (in the form of increased cost and time spent) in the safeguards technology design process. Here, to mitigate this knowledge gap, the Y-12 National Security Complex in Oak Ridge, Tennessee, has developed design considerations that should be incorporated into safeguards technologies, focusing on non-destructive assay equipment. Timelines for integration of these considerations into the design process are also discussed.

98 NUCLEAR DISARMAMENT, SAFEGUARDS, AND PHYSICAL P↗