Results for “suitability” — Page 2

DOE OSTI2023

Integrated Land Suitability Assessment for Depots Siting in a Sustainable Biomass Supply Chain

A sustainable biomass supply chain would require not only an effective and fluid transportation system with a reduced carbon footprint and costs, but also good soil characteristics ensuring durable biomass feedstock presence. Unlike existing approaches that fail to account for ecological factors, this work integrates ecological as well as economic factors for developing sustainable supply chain development. For feedstock to be sustainably supplied, it necessitates adequate environmental conditions, which need to be captured in supply chain analysis. Using geospatial data and heuristics, we present an integrated framework that models biomass production suitability, capturing the economic aspect via transportation network analysis and the environmental aspect via ecological indicators. Production suitability is estimated using scores, considering both ecological factors and road transportation networks. These factors include land cover/crop rotation, slope, soil properties (productivity, soil texture, and erodibility factor) and water availability. This scoring determines the spatial distribution of depots with priority to fields scoring the highest. Two methods for depot selection are presented using graph theory and a clustering algorithm to benefit from contextualized insights from both and potentially gain a more comprehensive understanding of biomass supply chain designs. Graph theory, via the clustering coefficient, helps determine dense areas in the network and indicate the most appropriate location for a depot. Clustering algorithm, via K-means, helps form clusters and determine the depot location at the center of these clusters. An application of this innovative concept is performed on a case study in the US South Atlantic, in the Piedmont region, determining distance traveled and depot locations, with implications on supply chain design. The findings from this study show that a more decentralized depot-based supply chain design with 3depots, obtained using the graph theory method, can be more economical and environmentally friendly compared to a design obtained from the clustering algorithm method with 2 depots. In the former, the distance from fields to depots totals 801,031,476 miles, while in the latter, it adds up to 1,037,606,072 miles, which represents about 30% more distance covered for feedstock transportation.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

NASA NTRS2011

Hyperspectral Mapping of the Invasive Species Pepperweed and the Development of a Habitat Suitability Model

Mapping and predicting the spatial distribution of invasive plant species is central to habitat management, however difficult to implement at landscape and regional scales. Remote sensing techniques can reduce the impact field campaigns have on these ecologically sensitive areas and can provide a regional and multi-temporal view of invasive species spread. Invasive perennial pepperweed (Lepidium latifolium) is now widespread in fragmented estuaries of the South San Francisco Bay, and is shown to degrade native vegetation in estuaries and adjacent habitats, thereby reducing forage and shelter for wildlife. The purpose of this study is to map the present distribution of pepperweed in estuarine areas of the South San Francisco Bay Salt Pond Restoration Project (Alviso, CA), and create a habitat suitability model to predict future spread. Pepperweed reflectance data were collected in-situ with a GER 1500 spectroradiometer along with 88 corresponding pepperweed presence and absence points used for building the statistical models. The spectral angle mapper (SAM) classification algorithm was used to distinguish the reflectance spectrum of pepperweed and map its distribution using an image from EO-1 Hyperion. To map pepperweed, we performed a supervised classification on an ASTER image with a resulting classification accuracy of 71.8%. We generated a weighted overlay analysis model within a geographic information system (GIS) framework to predict areas in the study site most susceptible to pepperweed colonization. Variables for the model included propensity for disturbance, status of pond restoration, proximity to water channels, and terrain curvature. A Generalized Additive Model (GAM) was also used to generate a probability map and investigate the statistical probability that each variable contributed to predict pepperweed spread. Results from the GAM revealed distance to channels, distance to ponds and curvature were statistically significant (p < 0.01) in determining the locations of suitable pepperweed habitats.

Pepperweed↗

DOE OSTI2025

Determination of a suitable molar absorption coefficient (ε) for lignin analysis of fibrous plants using the CASA method

In this study, the benchmarking and applicability of the CASA method for the analysis of fibrous plants, such as flax, hemp, and jute, is evaluated. Lignin is a phenolic biopolymer present in plant cell walls and is composed of three primary monomeric units, designated G, S, and H. Various factors, such as the genetic variability, influence the relative proportions of these units in plant samples. Recently, the cysteine-assisted sulfuric acid (CASA) method has been introduced as a rapid method for the quantification of lignin in wood samples. The aim of this study is to establish a suitable molar absorption coefficient (ε, L·g –1 ·cm –1 ) to adapt the CASA method for use with annual plant fibers. This investigation was motivated by the technical advantages of the CASA method, including higher throughput, lower reaction temperatures, and ecological benefits due to the absence of carcinogenic, mutagenic, or reprotoxic (CMR) substances and the need for minimal sample quantities. In this method, lignin solubilization is facilitated by cysteine, which is an amino acid that enhances the reaction kinetics, using a one-hour incubation period. However, as with any spectrophotometric technique, the CASA method depends on a molar absorption coefficient (ε) that varies according to the ratio of the aromatic units within the polymer. To evaluate the suitability of CASA for quantifying lignin in economically significant plant fibers, we investigate the impact of the unit ratios on the accuracy of ε. The results are compared with those of two widely recognized lignin analytical methods: the Klason method, which is a gravimetric reference method, and the acetyl bromide soluble lignin method, which is the most commonly used spectrophotometric approach. The final ε obtained in this study reveals a relative difference in lignin content ranging from –8 % to +9 % based on a comparison between the CASA and Klason methods across different industrial hemp varieties. As a result, using known G:S ratios in annual fibrous plants, ε can be estimated from our results.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Developing Non-Food Grade Brassica Biofuel Feedstock Cultivars with High Yield, Oil Content, and Oil Quality that are Suitable for Low Input Production Dryland Systems (Final Report)

The U.S. uses a substantial amount of fossil fuel as an energy source for a wide range for functions including home heating, agriculture and transportation. In the transportation sector, diesel and jet fuel are consumed at a rapid rate, and alternative liquid energy is being investigated globally and nationally to reduce our dependence on fossil fuel and reduce the impact of our carbon footprint on global climate change. Non-food Brassica crops have the potential of producing high oil yield (over 250 gal acre-1) and have oil quality highly desirable for use as biodiesel or bio jet fuel. Developing oilseed feedstock Brassica cultivars with higher seed and oil yield, with high oil quality and with resistance to pathogens, that can be grown with few chemical inputs will helping break our dependence on fossil fuels and reduce importation of fossil fuels. While some oilseed Brassicas have been grown on a small scale for many years in the Pacific Northwest (PNW), adoption has been limited, and the potential of the crops have not been realized or even fully investigated. This report summarizes the results of a study to develop superior non-food grade winter (B. napus) and spring (B. napus and B. juncea) oilseed cultivars suitable for a range of PNW, and other US environments with high resistance to the biotic and abiotic stresses suitable for high-quality biofuel feedstocks. In conducting this work, genome-wide association selection was used to dissect the genetic architecture of industrial Brassica oilseed germplasm for yield, quality, and resistance to biotic and abiotic stresses. A genome-wide bioinformatics approach was used to identify putative PRR (pattern recognition receptor) - type resistance genes that confer durable resistance to blackleg. A novel transgenic approach was developed to generate resistant non-food oilseed lines using PPR genes Br1033 and Br8486. These genes were inserted into regionally adapted oilseed cultivars.

09 BIOMASS FUELS↗

DOE OSTI2025

Corral Summit Pumped Storage Hydropower Hybrid: Site Suitability Assessment

In 2024, Idaho National Laboratory (INL) and Pacific Northwest National Laboratory (PNNL) initiated a technical-assistance project to support Cat Creek Energy, LLC, (CCE) in evaluating site suitability for the proposed Corral Summit Pumped Storage Hydropower (PSH) project in south-central Idaho near Mackay Reservoir. The Corral Summit facility incorporates battery storage and photovoltaic (PV) solar arrays in a Trybrid configuration to deliver large-volume long-duration (LVLD) storage solutions for rural electric cooperatives in eastern Idaho. The evaluation process focused on determining the most-suitable location for the upper reservoir of the PSH system, guided by a comprehensive assessment framework spanning multiple categories, including physical characteristics, environmental constraints, building infrastructure, regulatory constraints, cultural resources and sensitivity, social factors, and power market and grid integration. Each site was analyzed based on a ranking scale (0–1), which scores ranging from “severely disfavored” to “highly favored,” allowing detailed comparisons of site-specific conditions. Categories such as hydraulic head, utilities corridor, land ownership, and transmission-grid limitations emerged as key contributors to the overall assessment. Site 2 (Idaho Trust) demonstrated a slight advantage over Site 1 (Bureau of Land Management) primarily due to favorable outcomes in regulatory constraints, building infrastructure, and power-market integration. However, Site 1 outperformed Site 2 in factors related to physical characteristics and social factors. The report emphasizes the need for further evaluation of both sites before clear determination of which site is preferred, due to the limited information available on either site at the time of this report. Key areas of evaluation to clearly define the preferred site are ecological impacts, cultural-resource surveys, and economic-feasibility assessments. For successful project execution, recommended follow-up actions include seismic and geotechnical surveys, groundwater and habitat monitoring, regulatory reviews of water rights and right-of-way agreements, cultural engagement with local tribal governments, and enhanced stakeholder strategies. These efforts will ensure the Corral Summit Trybrid facility meets local energy needs while balancing environmental, social, and regulatory responsibilities.

13 - HYDRO ENERGY↗

DOE OSTI2025

Corral Summit Pumped Storage Hydropower Hybrid Site Suitability Assessment (Rev.1)

In 2024, Idaho National Laboratory (INL) and Pacific Northwest National Laboratory (PNNL) initiated a technical-assistance project to support Cat Creek Energy, LLC, (CCE) in evaluating site suitability for the proposed Corral Summit Pumped Storage Hydropower (PSH) project in south-central Idaho near Mackay Reservoir. The Corral Summit facility incorporates battery storage and photovoltaic (PV) solar arrays in a Trybrid configuration to deliver large-volume long-duration (LVLD) storage solutions for rural electric cooperatives in eastern Idaho. The evaluation process focused on determining the most-suitable location for the upper reservoir of the PSH system, guided by a comprehensive assessment framework spanning multiple categories, including physical characteristics, environmental constraints, building infrastructure, regulatory constraints, cultural resources and sensitivity, social factors, and power market and grid integration. Each site was analyzed based on a ranking scale (0–1), which scores ranging from “severely disfavored” to “highly favored,” allowing detailed comparisons of site-specific conditions. Categories such as hydraulic head, utilities corridor, land ownership, and transmission-grid limitations emerged as key contributors to the overall assessment. Site 2 (Idaho Trust) demonstrated a slight advantage over Site 1 (Bureau of Land Management) primarily due to favorable outcomes in regulatory constraints, building infrastructure, and power-market integration. However, Site 1 outperformed Site 2 in factors related to physical characteristics and social factors. The report emphasizes the need for further evaluation of both sites before clear determination of which site is preferred, due to the limited information available on either site at the time of this report. Key areas of evaluation to clearly define the preferred site are ecological impacts, cultural-resource surveys, and economic-feasibility assessments. For successful project execution, recommended follow-up actions include seismic and geotechnical surveys, groundwater and habitat monitoring, regulatory reviews of water rights and right-of-way agreements, cultural engagement with local tribal governments, and enhanced stakeholder strategies. These efforts will ensure the Corral Summit Trybrid facility meets local energy needs while balancing environmental, social, and regulatory responsibilities.

13 - HYDRO ENERGY↗

DOE OSTI2024

Bird Species Use of Bioenergy Croplands in Illinois, USA—Can Advanced Switchgrass Cultivars Provide Suitable Habitats for Breeding Grassland Birds?

Grassland birds have sustained significant population declines in the United States through habitat loss, and replacing lost grasslands with bioenergy production areas could benefit these species and the ecological services they provide. Point count surveys and autonomous acoustic monitoring were used at two field sites in Illinois, USA, to determine if an advanced switchgrass cultivar that is being used for bioenergy feedstock production could provide suitable habitats for grassland and other bird species. At the Brighton site, the bird use of switchgrass plots was compared to that of corn plots during the breeding seasons of 2020–2022. At the Urbana site, the bird use of restored prairie, switchgrass, and Miscanthus × giganteus was studied in the 2022 breeding season. At Brighton, Common Yellowthroat, Dickcissel, Grasshopper Sparrow, and Sedge Wren occurred on switchgrass plots more often than on corn; Common Yellowthroat and Dickcissel increased on experimental plots as the perennial switchgrass increased in height and density over the study period; and the other two species declined over the same period. At Urbana, Dickcissel was most frequent in prairie and switchgrass; Common Yellowthroat was most frequent in miscanthus and switchgrass. These findings suggest that advanced switchgrass cultivars could provide suitable habitats for grassland birds, replace lost habitats, and contribute to the recovery of these vulnerable species.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2025

High Resolution Siting Suitability of Various Power Plant Technologies

Energy sector planning models determine the aggregate need for new generation, but these models are typically at the state or regional scale and are not equipped to address the wide range of location- and technology-specific issues that are increasingly a factor in power plant siting. These animations demonstrate the aggregate siting suitability of various power plant technology configurations, considering technology-specific factors that can prohibit development. The data presented is from the GRIDCERF (Geospatial Raster Input Data for Capacity Expansion Regional Feasibility) data package. GRIDCERF is a harmonized, open-source geospatial product that can be used to evaluate siting suitability for renewable and non-renewable power plants in the conterminous United States. The animations presented here demonstrate a curated selection of the full suite of technology configurations available. GRIDCERF provides the necessary inputs for models that simulate power plant siting for regional capacity expansion planning such as the Capacity Expansion Regional Feasibility (CERF) model.

Mongird, Kendall [Pacific Northwest National Labor↗

NASA NTRS1986

The suitability of various spacecraft for future space applications missions

The Space Applications Advisory Committee (SAAC) of NASA's Advisory Council was asked by the Associate Administrator for Space Science and Applications to consider the most suitable future means for accomplishing space application missions. To comply with this request, SAAC formed a Task Force whose report is contained in this document. In their considerations, the Task Force looked into the suitability of likely future spacecraft options for supporting various types of application mission payloads. These options encompass a permanent manned space station, the Space Shuttle operating in a sortie mode, unmanned platforms that integrate a wide variety of instruments or other devices, and smaller free fliers that accommodate at most a few functions. The Task Force also recognized that the various elements could be combined to form a larger space infrastructure. This report summarizes the results obtained by the Task Force. It describes the approach utilized, the findings and their analysis, and the conclusions.

Mathews, C. W.↗

NASA NTRS1990

Laser ablated high T(sub c) superconducting thin YBa2Cu3O(7-x) films on substrates suitable for microwave applications

The development of high temperature superconducting YBa2Cu3O(7-x) thin films on substrates suitable for microwave applications is of great interest for evaluating their applications for space radar, communication, and sensor systems. Thin films of YBa2Cu3O(7-x) were formed on SrTiO3, ZrO2, MgO, and LaAlO3 substrates by laser ablation. The wavelength used was 248 nm from a KrF excimer laser. During deposition the films were heated to 600 C in a flowing oxygen environment, and required no post annealing. The low substrate temperature during deposition with no post annealing gave films which were smooth, which had their c-axis aligned to the substrates, and which had grains ranging from 0.2 to 0.5 microns in size. The films being c-axis aligned gave excellent surface resistance at 35 GHz which was lower than that of copper at 77 K. At present, LaAlO3 substrates with a dielectric constant of 22, appears suitable as a substrate for microwave and electronic applications. The films were characterized by resistance-temperature measurements, scanning electron microscopy, and x ray diffraction. The highest critical transition temperatures (T sub c) are above 89 K for films on SrTiO3 and LaAlO3, above 88 K for ZrO2, and above 86 K for MgO. The critical current density (J sub c) of the films on SrTiO3 is above 2 x 10(exp 6) amperes/sq cm at 77 K. The T(sub c) and J(sub c) are reported as a function of laser power, composition of the substrate, and temperature of the substrate during deposition.

Warner, J. D.↗

NASA NTRS2005

A Tamarisk Habitat Suitability Map for the Continental US

This paper presents a national-scale map of habitat suitability for a high-priority invasive species, Tamarisk (Tamarisk spp., salt cedar). We successfully integrate satellite data and tens of thousands of field sampling points through logistic regression modeling to create a habitat suitability map that is 90% accurate. This interagency effort uses field data collected and coordinated through the US Geological Survey and nation-wide environmental data layers derived from NASA s MODerate Resolution Imaging Spectroradiometer (MODIS). We demonstrate the utilization of the map by ranking the lower 48 US states (and the District of Columbia) based upon their absolute, as well as proportional, areas of highly likely and moderately likely habitat for Tamarisk. The interagency effort and modeling approach presented here could be applied to map other harmful species in the US and globally.

Morisette, Jeffrey T.↗

NASA NTRS2005

Predicting Software Suitability Using a Bayesian Belief Network

The ability to reliably predict the end quality of software under development presents a significant advantage for a development team. It provides an opportunity to address high risk components earlier in the development life cycle, when their impact is minimized. This research proposes a model that captures the evolution of the quality of a software product, and provides reliable forecasts of the end quality of the software being developed in terms of product suitability. Development team skill, software process maturity, and software problem complexity are hypothesized as driving factors of software product quality. The cause-effect relationships between these factors and the elements of software suitability are modeled using Bayesian Belief Networks, a machine learning method. This research presents a Bayesian Network for software quality, and the techniques used to quantify the factors that influence and represent software quality. The developed model is found to be effective in predicting the end product quality of small-scale software development efforts.

Beaver, Justin M.↗

NASA NTRS2012

The Invasive Species Forecasting System (ISFS): An iRODS-Based, Cloud-Enabled Decision Support System for Invasive Species Habitat Suitability Modeling

The Invasive Species Forecasting System (ISFS) is an online decision support system that allows users to load point occurrence field sample data for a plant species of interest and quickly generate habitat suitability maps for geographic regions of interest, such as a national park, monument, forest, or refuge. Target customers for ISFS are natural resource managers and decision makers who have a need for scientifically valid, model- based predictions of the habitat suitability of plant species of management concern. In a joint project involving NASA and the Maryland Department of Natural Resources, ISFS has been used to model the potential distribution of Wavyleaf Basketgrass in Maryland's Chesapeake Bay Watershed. Maximum entropy techniques are used to generate predictive maps using predictor datasets derived from remotely sensed data and climate simulation outputs. The workflow to run a model is implemented in an iRODS microservice using a custom ISFS file driver that clips and re-projects data to geographic regions of interest, then shells out to perform MaxEnt processing on the input data. When the model completes, all output files and maps from the model run are registered in iRODS and made accessible to the user. The ISFS user interface is a web browser that uses the iRODS PHP client to interact with the ISFS/iRODS- server. ISFS is designed to reside in a VMware virtual machine running SLES 11 and iRODS 3.0. The ISFS virtual machine is hosted in a VMware vSphere private cloud infrastructure to deliver the online service.

Gill, Roger↗

NASA NTRS2020

Southern Bhutan Ecological Forecasting Tech Paper: Modeling Asian Elephant (Elephas maximus) Habitat Suitability along the Southern Bhutan Border with NASA Earth Observations

Asian elephants (Elephas maximus) are a flagship species essential for the functioning of forest ecosystems, and they also have cultural significance in Bhutan. Elephants receive the highest legal protection as listed under Schedule I of the Bhutan Forests and Nature Conservation Act, 1995. Yet, they face threats of extinction due to poaching for ivory as well as the loss and fragmentation of their habitat. Due to the recent clearing of forests and the growing populations in these areas, there has been an increase in incidents of human-elephant conflict. These conflicts have been detrimental to farmer’s annual harvests and livelihoods and have led to retaliatory killing and injury of elephants in southern Bhutan. The DEVELOP Southern Bhutan Ecological Forecasting team partnered with the Bhutan Foundation and Bhutan Tiger Center to help address this problem. The team integrated NASA Earth observations, including Landsat 5 Thematic Mapper (TM), Landsat 8 Operational Land Imager (OLI), Terra Moderate Resolution Imaging Spectroradiometer (MODIS), and the Shuttle Radar Topography Mission (SRTM) to acquire information on land cover change and elephant habitat suitability along the southern border of Bhutan. The team utilized Esri ArcGIS Pro and Software for Assisted Habitat Modeling (SAHM) for data analysis, modeling, and visualization. The team used elephant occurrence data and environmental variables to model current habitat suitability for migrating elephant populations. This analysis provided partners with maps to inform decisions about the placement and conservation of elephant corridors and helped build their capacity to use satellite data for future studies and project planning.

Palchen Wangchuk↗

NASA NTRS2020

Southern Bhutan Ecological Forecasting Project Summary - Modeling Asian Elephant (Elephas Maximus) Habitat Suitability along the Southern Bhutan Border with NASA Earth Observations

Asian elephants (Elephas maximus) are a flagship species essential for the functioning of forest ecosystems, and they also have cultural significance in Bhutan. Elephants receive the highest legal protection as listed under Schedule I of the Bhutan Forests and Nature Conservation Act, 1995. Yet, they face threats of extinction due to poaching for ivory as well as the loss and fragmentation of their habitat. Due to the recent clearing of forests and the growing populations in these areas, there has been an increase in incidents of human-elephant conflict. These conflicts have been detrimental to farmer’s annual harvests and livelihoods and have led to retaliatory killing and injury of elephants in southern Bhutan. The DEVELOP Southern Bhutan Ecological Forecasting team partnered with the Bhutan Foundation and Bhutan Tiger Center to help address this problem. The team integrated NASA Earth observations, including Landsat 5 Thematic Mapper (TM), Landsat 8 Operational Land Imager (OLI), Terra Moderate Resolution Imaging Spectroradiometer (MODIS), and the Shuttle Radar Topography Mission (SRTM) to acquire information on land cover change and elephant habitat suitability along the southern border of Bhutan. The team utilized Esri ArcGIS Pro and Software for Assisted Habitat Modeling (SAHM) for data analysis, modeling, and visualization. The team used elephant occurrence data and environmental variables to model current habitat suitability for migrating elephant populations. This analysis provided partners with maps to inform decisions about the placement and conservation of elephant corridors and helped build their capacity to use satellite data for future studies and project planning.

DEVELOP Project Summary↗

NASA NTRS2021

Detect-and-Avoid Safety and Operational Suitability Analysis using an Electro-Optical/Infared Sensor Model

This report documents a closed-loop analysis of an DAA system using a Electro/Optical sensor model. Safety and operational suitability metrics are computed. Results show that the safety metrics are most sensitive to the angular rate accuracy of the intruder aircraft. Trade-off between safety and operationl suitability metrics is discussed. Results from this work directly inform the requirements of an electro/optical sensors for detect-and-avoid.

Detect-and-avoid↗

NASA NTRS2022

Northeast US Ecological Forecasting: Modeling Invasive Plant Habitat Suitability to Support Management Efforts in the American Northeast

Invasive plant species threaten environmental and economic interests when they spread into new areas, outcompete native species, and disrupt ecosystem services. If the spread is not controlled early, species can become well-established and increasingly difficult to manage. The National Park Service (NPS) Invasive Plant Management Teams (IPMTs) strive for an “early detection, rapid response” approach to reducing invasive species spread. Management teams can better prioritize their work with the help of species distribution models (SDMs), which map habitat suitability by combining species occurrences with environmental predictor variables. Scarce invaded range data for newly arrived invasive species presents a particular challenge for producing accurate models. To improve future modeling efforts, this project compared SDM methods using different spatial scales to model two plant species invasive to the Northeast US: the well-established Japanese stiltgrass (Microstegium vimineum) and newer invasive species wavyleaf basketgrass (Oplismenus undulatifolius). The team used NASA Earth observations and climate datasets to model occurrence data and predictor layers at a US-specific extent (90m2 spatial resolution) and global extent (1 km2 spatial resolution). Landsat 5 Thematic Mapper (TM), Landsat 7 Enhanced Thematic Mapper Plus (ETM+), and Landsat 8 Operational Land Imager (OLI) provided data for US Normalized Difference Moisture Indices (NDMI), while global NDMI and topographic predictor layers were derived from Shuttle Radar Topography Mission (SRTM) and Terra Moderate Resolution Imaging Spectroradiometer (MODIS). The resulting models indicated important predictor variables for each species and explored the benefits and tradeoffs of using global data to model habitat suitability for new-arrival invasive species.

Rebecca Ohman↗

NASA NTRS2023

Palmer Station, Antarctica: A Ground-Based Spaceflight Analog Suitable for Validation of Biomedical Countermeasures for Deep Space Missions

Astronauts are known to exhibit a variety of immunological alterations during spaceflight including changes in leukocyte distribution and plasma cytokine concentrations, a reduction in T-cell function, and subclinical reactivation of latent herpesviruses. These alterations are most likely due to mission-associated stressors including circadian misalignment, microgravity, isolation, altered nutrition, and increased exposure to cosmic radiation. Some of these stressors may also occur in terrestrial situations. This study sought to determine if crewmembers performing overwinter deployment at Palmer Station, Antarctica displayed similar immune alterations. The larger goal was to validate a ground analog suitable for the evaluation of countermeasures designed to protect astronauts during future deep space missions. For this pilot study, plasma, saliva, hair, and health surveys were collected from Palmer Station, Antarctica winterover participants at baseline, and at five overwinter timepoints. Twenty-six subjects consented to participate over the course of two seasons. Initial sample processing was performed at Palmer, and eventually stabilized samples were returned to the Johnson Space Center for analysis. A white blood cell differential was performed (real time) using a fingerstick blood sample to determine alterations in basic leukocyte subsets throughout the winterover. Plasma and saliva samples were analyzed for 30 and 13 cytokines, respectively. Saliva was analyzed for cortisol concentration and three latent herpesviruses (DNA by qPCR), EBV, HSV1, and VZV. Hair samples were analyzed for several hormones, as a measure of stress over prolonged periods of time. Voluntary surveys related to general health and adverse clinical events were distributed to participants. It is noteworthy that due to logistical constraints due to COVID-19, the baseline samples for each season were collected in Punta Arenas, Chile, after long international travel and during isolation. Therefore, the palmer pre mission samples may not reflect a true normal ‘baseline’. Minimal alterations were observed in leukocyte distribution during overwinter. The mean percentage of monocyte concentration elevated at one timepoint. Plasma G-CSF, IL1RA, MCP-1, MIP-1β, TNFα and VEGF were decreased during at least one overwinter timepoint, whereas RANTES was significantly increased. No statistically significant changes were observed in mean saliva cytokine concentrations. Salivary cortisol was substantially elevated throughout the entire winterover compared to baseline. Compared to shedding levels observed in healthy controls (23%), the percentage of participants who shed EBV was higher throughout all winterover timepoints (52-60%). Five subjects shed HSV1 during at least one timepoint throughout the season compared to no subjects shedding during pre-deployment. Finally, VZV reactivation, common in astronauts but exceptionally rare in ground-based stress analogs, was observed in one subject during pre-deployment and a different subject at WO2 and WO3. These pilot data, somewhat influenced by the COVID-19 situation, do suggest that participants at Palmer Station do undergo immunological alterations similar to, but likely in reduced magnitude, as those observed in astronauts. We suggest that overwinter at Palmer Station may be suitable test analog for spaceflight biomedical countermeasures designed to mitigate clinical risks for deep space missions.

Space↗