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All sourcesNASA NTRSDOE OSTI
NASA NTRS2019

Pre-Demonstration Development of Controlled-Release Corrosion Inhibitors and Healing Agents as Alternatives to Hexavalent Chromium

This Limited Scope Study was directed to achieve the following objectives: (1) Scale-up of materials that can meet MIL-PRF-23377 (solvent-based primer). (2) Provide evidence of resistance to aircraft alkaline cleaners and de-icing fluids. (3) Provide formulation for initial ecological and toxicity screening. (4) Submit an interim report that will provide the basis for a future ESTCP (the Dept. of Defense's (DoD's) Environmental Security Technology Certification Program) demonstration effort.The encapsulation procedure for two corrosion inhibitors was scaled up from lab scale to 2.0 kilograms. This scale will accommodate high volume production of coatings with encapsulated corrosion inhibitors for large surface areas during a follow-on ESTCP demonstration effort. Test results provided evidence of resistance to alkaline cleaners and aircraft de-icing fluids and compliance with MIL-PRF-23377. All the MIL-PRF-23377 requirements were met with the exception of the adhesion requirement, in top coated panels, and the flexibility requirement. Work on solving these two problems will be done prior to validation and demonstration of the technology. An initial ecological and toxicity screening identified several factors that were not critical to the acceptance of the encapsulated corrosion inhibitor. This new technology will lead to environmentally friendly alternatives to hexavalent chromium that will enable DoD to protect its assets. Field demonstration, licensing, qualification, and commercialization will allow its real world utilization. Abstract for Appendix A. Toxicology Report: Corrosion controls a significant problem for military equipment. Many surface treatment materials currently in use on items of military or aerospace materiel contain chromium (VI) as a component, a source of significant human health and environmental hazard. The formulation under evaluation contains no chromium. Most of the proposed components are low to moderate toxicity and are not a significant concern. Two of the formulation components are associated with some cancer hazard - 2-mercaptobenzothiazole and formaldehyde, but both find widespread use in industry and additional investigation is not required for this project.

Calle, Luz Marina↗
NASA NTRSDate not supplied

Molten Salt Lattice Confinement Fusion (LCF) Fast Fission Reactor for Lunar and Planetary Surface Power

Molten salt fission reactors (MSR) have been suggested for lunar and planetary surface power systems. They have the advantage of operating at high temperature, for efficient thermal-electrical conversion, low pressure, long-lived with high nuclear fuel burnup. MSR are often designed to breed fissile 233U from natural 232Th by neutron capture and  decay via: 232Th(n,)233Th(,)233Pa(,)233U Unfortunately, this process requires 233Pa isotope separation and segregation to decay to 233U. This requirement prevents additional neutron capture that interferes with 233U breeding. Instead, Wooley’s sub-critical, fast fission, molten salt reactor would use externally generated tokamak fusion neutrons1,2 to fission all actinides.We propose a simpler fusion-fast-fission sub-critical reactor that generates fast neutrons in situ from lattice confinement fusion (LCF) to fission fertile and fissile actinides. This hybrid reactor doesn't require enriched 235U fissile pins to initiate fis-sion reactions, nor 233Pa separation and segregation during operation. Like Wooley’s, this hybrid reactor “burns” natural uranium (238U) or thorium (232Th) which avoids uranium enrichment and additional fissile material launch safety and security costs. The LCF neutron source is initiated by bremsstrahlung photoneutrons (Fig. 1)3 or isotopic neutron sources in electron-screened lattices (Fig. 2)4,5. Alternatively, the electrolytic Pd-deuterium co-deposition6protocol fast fissions7 both 232Th and 238U. However, an aqueous electrolyte-based system, without pressurization similar to conventional pressurized water fission reactors, is incapable of high temperatures due to the boiling point of the electrolyte slightly over 100 C. Molten salts can be used instead as was demonstrated at the University of Hawaii8 using a variety of Ni and Pd cathodes in lithiated, hydrided and deuterated salts. These salts have melting points often exceeding 500C making them suitable to efficiently produce electrical power9 through either Advanced Stirling Genera-tors (< 100 kWe) or closed-Brayton Cycle (> 100 kWe). This hybrid reactor could power a wide range of lunar or Martian applications from unmanned in-struments, to charging vehicles and entire facilities such as human habitats or in situ resource utilization. The power conversion cycles are Carnot Cycle limited, but generally 30% efficient at best. However, waste heat on the moon or Mars is important to surviving either two-week lunar nights or Martian nights as well as providing process heat for mineral extraction and “living off the land”

Lawrence Forsley↗
NASA NTRS1991

Aero/fluids database system

The AFAS Database System was developed to provide the basic structure of a comprehensive database system for the Marshall Space Flight Center (MSFC) Structures and Dynamics Laboratory Aerophysics Division. The system is intended to handle all of the Aerophysics Division Test Facilities as well as data from other sources. The system was written for the DEC VAX family of computers in FORTRAN-77 and utilizes the VMS indexed file system and screen management routines. Various aspects of the system are covered, including a description of the user interface, lists of all code structure elements, descriptions of the file structures, a description of the security system operation, a detailed description of the data retrieval tasks, a description of the session log, and a description of the archival system.

Reardon, John E.↗
NASA NTRS2015

Reducing the Effect of Transducer Mount Induced Noise on Aeroacoustic Wind Tunnel Testing Data with a New Transducer Mount Design

Characterization of launch vehicle unsteady aerodynamics is a field best studied through experimentation, which is often carried out in the form of large scale wind tunnel testing. Measurement of the fluctuating pressures induced by the boundary layer noise is customarily made with miniature pressure transducers installed into a model of the vehicle of interest. Literature shows that noise level increases between two to five decibels (dB referenced to 20 micropascal) can be induced when the transducer surface is not mounted perfectly flush with the model outer surface. To reduce this artificially induced noise, special transducer holders have been used for aeroacoustic wind tunnel testing by NASA. This holder is a sleeve into which the transducer fits, with a cap that allows it to be mounted in a recessed hole in the model. A single hole in the holder allows the transport of the tunnel medium so the transducer can discriminate the fluctuating pressure due to the turbulent boundary layer noise. The holder is first dry fitted into the model and any difference in height between the holder and the model surface can be sanded flush. The holder is then removed from the model, the transducer glued inside the holder, and the holder replaced in the model, secured also with glue, thus eliminating the problem of noise level increases due to lack of flushness. In order to work with this holder design, special transducers have been ordered with their standard screen removed and the diaphragm moved as close to the top of the casing as possible to minimize any cavity volume. Although this greatly reduces induced noise due to the transducers being out of flush, the holders can also induce a cavity resonance that is usually at a very high frequency. This noise is termed transducer mount induced noise (XMIN). The peak of the mode can vary with the cavity depth, boundary layer noise that can excite the mode, tunnel flow medium, and the build of the transducers. Because the boundary layer flow interaction with the microphone holder seems to have some effect on the cavity resonance response, the NASA/Marshall Space Flight Center (MSFC) aeroacoustic team postulated that changing the design of the holder might reduce the cavity resonance response. An experiment was performed at the MSFC Trisonic Wind Tunnel comparing a new holder design with the traditional MSFC holder. The new holder design is intended to mimic the Kulite Semiconductors, Inc. B-screen, where the single hole of the traditional MSFC holder is replaced by a series of ten 0.007 inch diameter holes. This particular design serves to protect the transducer diaphragm as in the manufacturer's B-screen, but also increases the frequency of the XMIN such that it is not apparent in the data collected from aeroacoustic wind tunnel tests. MSFC compared the two holder designs by installing two transducers in standard holders on one side of a ten inch long ten degree cone, and two transducers in B-screen holders on the opposite side for a series of runs at different conditions. Over 11-13 February, 2013, the model was run at Mach numbers between 0.80 and 1.96 at angles of attack at 0 deg, plus or minus 1 deg, plus or minus 2 deg, plus or minus 4 deg, and plus or minus 8 deg. Model roll attitudes at 0 deg, 90 deg, and 180 deg were tested to isolate potential tunnel bias. This paper presents results from the test showing that the proposed holder design significantly reduces the influence of XMIN on measured fluctuating pressure levels.

Herron, A. J.↗
NASA NTRS2021

Remote Concurrent Engineering: A-Team Studies in the Virtual World

NASA Jet Propulsion Laboratory’s (JPL’s)Architecture Team (A-Team) has nearly a decade of experiencein maturing early formulation mission and technology conceptsby combining innovative collaborative engineering methodswith cutting-edge subject matter expertise and advancedanalysis tools in an in-person environment. When COVID-19forced JPL’s workforce to work remotely in March 2020, ATeamhad to quickly pivot from an in-person collaborativeenvironment to a remote working environment.Through introspection, careful planning, and considerablepractice, A-Team was able to develop new operating proceduresto effectively continue early formulation studies in a virtualenvironment. A-Team has held over 57 remote studies in the 10months since the start of mandatory telework at JPL in March2020. In the remote setting, A-Team conducts studies in half-daysessions with clients and subject matter experts (SMEs) viavideoconferencing, shared computer screens, and digitalcollaborative tools.The key lesson is that increased staffing and planning is neededto prepare and successfully run remote A-Team studies. RemoteA-Team studies require careful selection of the appropriatetools for security, accessibility, and usability within theNASA/JPL environment. Knowledge capture methods andtemplates need to be thought out and agreed upon in advance asthere is less room for improvising in a remote format. Variouscommunication channels have to be monitored to allow for teamcoordination while maintaining fruitful participant engagementduring a session. In addition, technical backup for all roleswithin the A-Team have to be identified to allow the study tocontinue even if a team member’s connectivity is temporarilyinterrupted. Finally, careful thought has to be put into methodsand processes to create a collaborative environment in a virtualspace such that a group of experts who are only connected viathe internet can experience the creative spark and flow of a greatcollaborative and innovative study.

Zusack, Steven↗
NASA NTRS2006

3D X-Ray Luggage-Screening System

A three-dimensional (3D) x-ray luggage- screening system has been proposed to reduce the fatigue experienced by human inspectors and increase their ability to detect weapons and other contraband. The system and variants thereof could supplant thousands of xray scanners now in use at hundreds of airports in the United States and other countries. The device would be applicable to any security checkpoint application where current two-dimensional scanners are in use. A conventional x-ray luggage scanner generates a single two-dimensional (2D) image that conveys no depth information. Therefore, a human inspector must scrutinize the image in an effort to understand ambiguous-appearing objects as they pass by at high speed on a conveyor belt. Such a high level of concentration can induce fatigue, causing the inspector to reduce concentration and vigilance. In addition, because of the lack of depth information, contraband objects could be made more difficult to detect by positioning them near other objects so as to create x-ray images that confuse inspectors. The proposed system would make it unnecessary for a human inspector to interpret 2D images, which show objects at different depths as superimposed. Instead, the system would take advantage of the natural human ability to infer 3D information from stereographic or stereoscopic images. The inspector would be able to perceive two objects at different depths, in a more nearly natural manner, as distinct 3D objects lying at different depths. Hence, the inspector could recognize objects with greater accuracy and less effort. The major components of the proposed system would be similar to those of x-ray luggage scanners now in use. As in a conventional x-ray scanner, there would be an x-ray source. Unlike in a conventional scanner, there would be two x-ray image sensors, denoted the left and right sensors, located at positions along the conveyor that are upstream and downstream, respectively (see figure). X-ray illumination may be provided by a single source or by two sources. The position of the conveyor would be detected to provide a means of matching the appropriate left- and right-eye images of an item under inspection. The appropriate right- and left-eye images of an item would be displayed simultaneously to the right and left eyes, respectively, of the human inspector, using commercially available stereo display screens. The human operator could adjust viewing parameters for maximum viewing comfort. The stereographic images thus generated would differ from true stereoscopic images by small distortions that are characteristic of radiographic images in general, but these distortions would not diminish the value of the images for identifying distinct objects at different depths.

Fernandez, Kenneth↗
NASA NTRS2012

FERMI/GLAST Integrated Trending and Plotting System Release 5.0

An Integrated Trending and Plotting System (ITPS) is a trending, analysis, and plotting system used by space missions to determine performance and status of spacecraft and its instruments. ITPS supports several NASA mission operational control centers providing engineers, ground controllers, and scientists with access to the entire spacecraft telemetry data archive for the life of the mission, and includes a secure Web component for remote access. FERMI/GLAST ITPS Release 5.0 features include the option to display dates (yyyy/ddd) instead of orbit numbers along orbital Long-Term Trend (LTT) plot axis, the ability to save statistics from daily production plots as image files, and removal of redundant edit/create Input Definition File (IDF) screens. Other features are a fix to address invalid packet lengths, a change in naming convention of image files in order to use in script, the ability to save all ITPS plot images (from Windows or the Web) as GIF or PNG format, the ability to specify ymin and ymax on plots where previously only the desired range could be specified, Web interface capability to plot IDFs that contain out-oforder page and plot numbers, and a fix to change all default file names to show yyyydddhhmmss time stamps instead of hhmmssdddyyyy. A Web interface capability sorts files based on modification date (with newest one at top), and the statistics block can be displayed via a Web interface. Via the Web, users can graphically view the volume of telemetry data from each day contained in the ITPS archive in the Web digest. The ITPS could be also used in nonspace fields that need to plot data or trend data, including financial and banking systems, aviation and transportation systems, healthcare and educational systems, sales and marketing, and housing and construction.

Ritter, Sheila↗
NASA NTRS2011

Deployment of an Advanced Electrocardiographic Analysis (A-ECG) to Detect Cardiovascular Risk in Career Firefighters

INTRODUCTION Sudden cardiac death is the leading cause of line of duty death among firefighters, accounting for approximately 45% of fatalities annually. Firefighters perform strenuous muscular work while wearing heavy, encapsulating personal protective equipment in high ambient temperatures, under chaotic and emotionally stressful conditions. These factors can precipitate sudden cardiac events like myocardial infarction, serious dysrhythmias, or cerebrovascular accidents in firefighters with underlying cardiovascular disease. Screening for cardiovascular risk factors is recommended but not always followed in this population. PHASER is a project charged with identifying and prioritizing risk factors in emergency responders. We have deployed an advanced ECG (A-ECG) system developed at NASA for improved sensitivity and specificity in the detection of cardiac risk. METHODS Forty-four professional firefighters were recruited to perform comprehensive baseline assessments including tests of aerobic performance and laboratory tests for fasting lipid profiles and glucose. Heart rate and conventional 12-lead ECG were obtained at rest and during incremental treadmill exercise testing (XT). In addition, a 5-min resting 12-lead A-ECG was obtained in a subset of firefighters (n=18) and transmitted over a secure networked system to a physician collaborator at NASA for advanced-ECG analysis. This A-ECG system has been proven, using myocardial perfusion and other imaging, to accurately identify a number of cardiac pathologies including coronary artery disease (CAD), left ventricular hypertrophy, hypertrophic cardiomyopathy, non-ischemic cardiomyopathy, and ischemic cardiomyopathy. RESULTS Subjects mean (SD) age was 43 (8) years, weight 91 (13) kg, and BMI of 28 (3) kg/square meter. Maximum oxygen uptake (VO2max) was 39 (9) ml/kg/min. This compares with the 45th %ile in healthy reference values and a recommended standard of 42 ml/kg/min for firefighters. The metabolic threshold (VO2Theta) above which lactate accumulates was 23 (8) ml/kg/min. The chronotropic index, a measure of cardiovascular strain during XT was 35 (8) /L compared with reference values for men of 40 /L. Total cholesterol, LDL-C and HDL-C were 202 (34),126 (29), and 55 (15) mg/dl, respectively. Fifty-one percent of subjects had .3 cardiovascular risk factors, 2 subjects had resting hypertension (BP.140/90), and 23 had pre-hypertension (.120/80 but <140/90). Seven had exaggerated exercise induced hypertension but only one had ST depression on XT ECG, at least one positive A-ECG score for CAD, and documented CAD based on cardiology referral. While all other subjects, including those with fewer risk factors, higher aerobic fitness, and normal exercise ECGs, were classified as healthy by A-ECG, there was no trend for association between risk factors and any of 20 A-ECG parameters in the grouped data. CONCLUSIONS A-ECG screening correctly identified the individual with CAD although there was no trend for A-ECG parameters to distinguish those with elevated BP or multiple risk factors but normal XT ECG. We have demonstrated that a new technology, advanced-ECG, can be introduced for remote firefighter risk assessment. This simple, time and cost-effective approach to risk identification that can be acquired remotely and transmitted securely can detect individuals potentially at risk for line-of-duty death. Additional research is needed to further document its value.

Dolezal, B. A.↗
NASA NTRS2011

Deployment of an Advanced Electrocardiographic Analysis (A-ECG) to Detect Cardiovascular Risk in Career Firefighters

INTRODUCTION: Sudden cardiac death is the leading cause of line of duty death among firefighters, accounting for approximately 45% of fatalities annually. Firefighters perform strenuous muscular work while wearing heavy, encapsulating personal protective equipment in high ambient temperatures, under chaotic and emotionally stressful conditions. These factors can precipitate sudden cardiac events like myocardial infarction, serious dysrhythmias, or cerebrovascular accidents in firefighters with underlying cardiovascular disease. PURPOSE: The purpose of this study was to deploy and then evaluate the contribution of resting advanced ECG (A-ECG) in addition to other screening tools (family history, lipid profiles, and cardiopulmonary exercise tests, XT) in assessment of an individual fs cardiac risk profile. METHODS: Forty-four career firefighters were recruited to perform comprehensive baseline assessments including tests of aerobic performance, fasting lipids and glucose. Five-min resting 12-lead A-ECGs were obtained in a subset of firefighters (n=21) and transmitted over a secure networked system to a NASA physician collaborator. Using myocardial perfusion and other imaging as the gold standard, A-ECG scoring has been proven useful in accurately identifying a number of cardiac pathologies including coronary artery disease (CAD), left ventricular hypertrophy, hypertrophic cardiomyopathy, and non-ischemic and ischemic cardiomyopathy. RESULTS: Subjects f mean (SD) age was 43 (8) years, weight 91 (13) kg, and BMI 28 (3) kg/m2. Fifty-one percent of subjects had .3 cardiovascular risk factors. One subject had ST depression on XT ECG, at least one positive A-ECG score for CAD, and documented CAD based on cardiology referral. While all other subjects, including those with fewer risk factors, higher aerobic fitness, and normal exercise ECGs, were classified as healthy by A-ECG, there was no trend for association between risk factors and any of 20 A-ECG parameters in the grouped data.

Dolezal, B. A.↗
NASA NTRS2006

The Ability of AMSTAR Dechlorination Solution to Remove and Degrade PCBs from Contaminated Surfaces

Polychlorinated biphenyls (PCBs) are a group of synthetic aromatic compounds with the general formula C12H(10-x)Cl(-x) that were historically used in industrial paints, caulking material and adhesives, as their properties enhanced structural integrity, reduced flammability and boosted antifungal properties. Although the United States Environmental Protection Agency (USEPA) has banned the manufacture of PCBs since 1979, they have been found in at least 500 of the 1,598 National Priorities List (Superfund) sites identified by the USEPA. Prior to the US EPA's ban on PCB production, PCBs were commonly used as additives in paints and asphalt-based adhesives that were subsequently applied to a variety of structures. Government facilities constructed as early as 1930 utilized PCB-containing binders or PCB-containing paints, which are now leaching into the environment and posing ecological and worker health concerns. In 2006, a commercially available product known as AMSTAR Dechlorination Solution was tested at NASA's Kennedy Space Center for its ability to remove and degrade PCBs from structural materials. This evaluation was requested by the Environmental Security Technology Certification Program (ESTCP) evaluating the ability of NASA's Bimetallic Treatment System (BTS) to remove and degrade PCBs from structural materials. The results of the laboratory testing are to be used to determine if a side-by-side field-scale test comparing BTS to AMSTAR was warranted. A recommended sampling and analysis testing program was submitted to ESTCP that included triplicate screening of AMSTAR's PCB dechlorination capabilities on a variety of surfaces including glass, bare metal, and painted metal coupons. The test procedures, analytical techniques and results obtained are presented in this interim report to ESTCP.

Quinn, Jacqueline E.↗
NASA NTRS2019

An Integrated Data Analytics Platform

An Integrated Science Data Analytics Platform is an environment that enables the confluence of resources for scientific investigation. It harmonizes data, tools and computational resources which subsequently enable the research community to focus on the investigation rather than spending time on security, data preparation, management, etc. OceanWorks is a NASA technology integration project to establish a cloud-based Integrated Ocean Science Data Analytics Platform at NASA’s Physical Oceanography Distributed Active Archive Center (PO.DAAC) for big ocean science. It focuses on advancement and maturity by bringing together several NASA open-source, big data projects for parallel analytics, anomaly detection, in-situ to satellite data matchup, quality-screened data subsetting, search relevancy, and data discovery. Our communities are relying on data distributed through data centers such as the PO.DAAC, COAPS, NCAR, and many others to conduct their research. In typical investigations, scientists would engage in: search for data, evaluate the relevance of that data, download it, and then apply algorithms to identify trends. Such workflow cannot scale if the research involves a massive amount of data or multi-variate measurements. NASA’s Surface Water and Ocean Topography (SWOT) mission is expected to produce massive amount of observational data during its 3-year nominal mission. Collections like SWOT challenges all existing Earth Science data archival, distribution and analysis paradigms. In this paper, we will discuss how OceanWorks enhances the analysis of physical ocean data where the computation is done on an elastic cloud platform next to the archive to deliver fast, web-accessible services for working with oceanographic measurements.

Yang, Chaowei↗
NASA NTRS2001

Summary of ADTT Website Functionality and Features

This report summarizes development of the ADTT web-based design environment by the ELORET team in 2000. The Advanced Design Technology Testbed had been in development for several years, with demonstration applications restricted to aerodynamic analyses of subsonic aircraft. The key changes achieved this year were improvements in Web-based accessibility, evaluation of collaborative visualization, remote invocation of geometry updates and performance analysis, and application to aerospace system analysis. Significant effort was also devoted to post-processing of data, chiefly through comparison of similar data for alternative vehicle concepts. Such comparison is an essential requirement for designers to make informed choices between alternatives. The next section of this report provides more discussion of the goals for ADTT development. Section 3 provides screen shots from a sample session in the ADTT environment, including Login and navigation to the project of interest, data inspection, analysis execution and output evaluation. The following section provides discussion of implementation details and recommendations for future development of the software and information technologies that provide the key functionality of the ADTT system. Section 5 discusses the integration architecture for the system, which links machines running different operating systems and provides unified access to data stored in distributed locations. Security is a significant issue for this system, especially for remote access to NAS machines, so Section 6 discusses several architectural considerations with respect to security. Additional details of some aspects of ADTT development are included in Appendices.

Hawke, Veronica↗
NASA NTRS1993

Opinion polls and the U.S. civil space program

The conclusions that can be drawn from public opinion polls depend a great deal on what usually does not appear on the newspaper page or television screen. Subtle biases can result from the population interviewed, the time of day individuals were called, how a particular question was asked, or how the answer was interpreted. Examples are the 1961 Gallop Poll, the survey done for Rockwell International by the firm of Yankelovich, Skelly and White/Clancy Shulman, and the one done by Jon D. Miller of the International Center for the Advancement of Scientific Literacy. There is more to learn from opinion polls than that a good proportion of adult Americans support the space program. We can learn that social and economic security are not competing goals with space, but interdependent goals. If we want to increase public support for space, we must increase the number of Americans who have the economic freedom to take an interest in something besides getting by, day after day. We can also learn that the majority of those who support the space program can distinguish between the bread and circuses of space travel. They are content to experience extraordinary adventures in the movie theaters; for their tax dollars they want real return in expended scientific knowledge and understanding. Finally, we can learn that we need to increase that return, not just for scientific careers, but for the ordinary people who pay our bills and for their children, our children. Ultimately, the space program is for them, as all investments in the future must be.

Kraemer, Sylvia K.↗
NASA NTRS2011

PMA-PhyloChip DNA Microarray to Elucidate Viable Microbial Community Structure

Since the Viking missions in the mid-1970s, traditional culture-based methods have been used for microbial enumeration by various NASA programs. Viable microbes are of particular concern for spacecraft cleanliness, for forward contamination of extraterrestrial bodies (proliferation of microbes), and for crew health/safety (viable pathogenic microbes). However, a "true" estimation of viable microbial population and differentiation from their dead cells using the most sensitive molecular methods is a challenge, because of the stability of DNA from dead cells. The goal of this research is to evaluate a rapid and sensitive microbial detection concept that will selectively estimate viable microbes. Nucleic acid amplification approaches such as the polymerase chain reaction (PCR) have shown promise for reducing time to detection for a wide range of applications. The proposed method is based on the use of a fluorescent DNA intercalating agent, propidium monoazide (PMA), which can only penetrate the membrane of dead cells. The PMA-quenched reaction mixtures can be screened, where only the DNA from live cells will be available for subsequent PCR reaction and microarray detection, and be identified as part of the viable microbial community. An additional advantage of the proposed rapid method is that it will detect viable microbes and differentiate from dead cells in only a few hours, as opposed to less comprehensive culture-based assays, which take days to complete. This novel combination approach is called the PMA-Microarray method. DNA intercalating agents such as PMA have previously been used to selectively distinguish between viable and dead bacterial cells. Once in the cell, the dye intercalates with the DNA and, upon photolysis under visible light, produces stable DNA adducts. DNA cross-linked in this way is unavailable for PCR. Environmental samples suspected of containing a mixture of live and dead microbial cells/spores will be treated with PMA, and then incubated in the dark. Thereafter, the sample is exposed to visible light for five minutes, so that the DNA from dead cells will be cross-linked. Following this PMA treatment step, the sample is concentrated by centrifugation and washed (to remove excessive PMA) before DNA is extracted. The 16S rRNA gene fragments will be amplified by PCR to screen the total microbial community using PhyloChip DNA microarray analysis. This approach will detect only the viable microbial community since the PMA intercalated DNA from dead cells would be unavailable for PCR amplification. The total detection time including PCR reaction for low biomass samples will be a few hours. Numerous markets may use this technology. The food industry uses spore detection to validate new alternative food processing technologies, sterility, and quality. Pharmaceutical and medical equipment companies also detect spores as a marker for sterility. This system can be used for validating sterilization processes, water treatment systems, and in various public health and homeland security applications.

Venkateswaran, Kasthuri J.↗
NASA NTRS2004

Distributive On-line Processing, Visualization and Analysis System for Gridded Remote Sensing Data

The ability to use data stored in the current Earth Observing System (EOS) archives for studying regional or global phenomena is highly dependent on having a detailed understanding of the data's internal structure and physical implementation. Gaining this understanding and applying it to data reduction is a time- consuming task that must be undertaken before the core investigation can begin. This is an especially difficult challenge when science objectives require users to deal with large multi-sensor data sets that are usually of different formats, structures, and resolutions, for example, when preparing data for input into modeling systems. The NASA Goddard Earth Sciences Data and Information Services Center (GES DISC) has taken a major step towards meeting this challenge by developing an infrastructure with a Web interface that allows users to perform interactive analysis online without downloading any data, the GES-DISC Interactive Online Visualization and Analysis Infrastructure or "Giovanni." Giovanni provides interactive, online, analysis tools for data users to facilitate their research. There have been several instances of this interface created to serve TRMM users, Aerosol scientists, Ocean Color and Agriculture applications users. The first generation of these tools support gridded data only. The user selects geophysical parameters, area of interest, time period; and the system generates an output on screen in a matter of seconds. The currently available output options are: Area plot averaged or accumulated over any available data period for any rectangular area; Time plot time series averaged over any rectangular area; Time plots image view of any longitude-time and latitude-time cross sections; ASCII output for all plot types; Image animation for area plot. In the future, we will add correlation plots, GIS-compatible outputs, etc. This allow user to focus on data content (i.e. science parameters) and eliminate the need for expensive learning, development and processing tasks that are redundantly incurred by an archive's user community. The current implementation utilizes the GrADS-DODS Server (GDS), a stable, secure data server that provides subsetting and analysis services across the Internet for any GrADS-readable dataset. The subsetting capability allows users to retrieve a specified temporal and/or spatial subdomain from a large dataset, eliminating the need to download everything simply to access a small relevant portion of a dataset. The analysis capability allows users to retrieve the results of an operation applied to one or more datasets on the server. In our case, we use this approach to read pre-processed binary files and/or to read and extract the needed parts from HDF or HDF-EOS files. These subsets then serve as inputs into GrADS processing and analysis scripts. It can be used in a wide variety of Earth science applications: climate and weather events study and monitoring; modeling. It can be easily configured for new applications.

Leptoukh, G.↗
NASA NTRSDate not supplied

Understanding Plant Nitrogen Form Preference Responses to Elevated CO2 Earth and Space Environments

Future long-duration missions to the International Space Station (ISS) and beyond will require a sustainable supply of food to support human crews. The spaceflight cabin environment often contains very high concentrations of CO2, and it is therefore crucial to understand plant responses to elevated CO2 (eCO2) environments. Much focus has been given to plant photosynthetic and performance parameters in response to eCO2, but studies on the effects of eCO2 on nitrogen uptake are poorly understood. Our previous work at The University of Sheffield, UK using novel stable isotope approaches has shown enhanced ammonium uptake and preference compared to nitrate at eCO2 in varieties of spring barley, hypothesized to be an indirect consequence of changes in photosynthesis and photorespiration. However, several varieties did not display altered preference under eCO2 and the universality of this response remains to be understood. In this NASA NPP project, several candidate crop species such as lettuce, radish and tomato will be screened using stable isotopes to assess whether N preference changes in favor of ammonium in response to eCO2 and whether this remains true at super-elevated CO2 (seCO2), which is often experienced on the ISS. Photosynthetic and related measurements will help to disentangle the relationship between these responses and photosynthesis. This work will enable the development of optimized nutrient regimes for candidate crops in space and in future Lunar and Martian habitats and will pave the way for selection of crop varieties adapted to an eCO2 and/or seCO2 environment. Moreover, this research will further our understanding of plant responses to the eCO2 environment brought about by climate change, allowing the development of future-proof crops that will help to maintain food security. This NPP Fellowship is funded by NASA Space Biology.

Luke Leslie Fountain↗
NASA NTRSDate not supplied

Understanding Plant Nitrogen Form Preference Responses to Elevated CO2 Earth and Spaceflight Cabin Environments

Future long-duration missions to the International Space Station (ISS) and beyond will require a sustainable supply of food to support human crews. The spaceflight cabin environment often contains very high concentrations of CO2, and it is therefore crucial to understand plant responses to elevated CO2 (eCO2) environments. Much focus has been given to plant photosynthetic and performance parameters in response to eCO2, but studies on the effects of eCO2 on nitrogen uptake are poorly understood. Our previous work at The University of Sheffield, UK using novel stable isotope approaches has shown enhanced ammonium uptake and preference compared to nitrate at eCO2 in varieties of spring barley, hypothesised to be an indirect consequence of changes in photosynthesis and photorespiration. However, several varieties did not display altered preference under eCO2 and the universality of this response remains to be understood. In this NASA NPP project, several candidate crop species such as lettuce, radish and tomato will be screened using stable isotopes to assess whether N preference changes in favor of ammonium in response to eCO2 and whether this remains true at super-elevated CO2 (seCO2), which is often experienced on the ISS. Photosynthetic and related measurements will help to disentangle the relationship between these responses and photosynthesis. This work will enable the development of optimized nutrient regimes for candidate crops in space and in future Lunar and Martian habitats and will pave the way for selection of crop varieties adapted to an eCO2 and/or seCO2 environment. Moreover, this research will further our understanding of plant responses to the eCO2 environment brought about by climate change, allowing the development of future-proof crops that will help to maintain food security. This NPP Fellowship is funded by NASA Space Biology.

Luke Fountain↗
NASA NTRSDate not supplied

Understanding Plant Nitrogen Form Preference Responses to Elevated CO2 Earth and Spaceflight Cabin Environments.

Future long-duration missions to the International Space Station (ISS) and beyond will require a sustainable supply of food to support human crews. The spaceflight cabin environment often contains very high concentrations of CO2, and it is therefore crucial to understand plant responses to elevated CO2 (eCO2) environments. Much focus has been given to plant photosynthetic and performance parameters in response to eCO2, but studies on the effects of eCO2 on nitrogen uptake are poorly understood. Our previous work at The University of Sheffield, UK using novel stable isotope approaches has shown enhanced ammonium uptake and preference compared to nitrate at eCO2 in varieties of spring barley, hypothesised to be an indirect consequence of changes in photosynthesis and photorespiration. However, several varieties did not display altered preference under eCO2 and the universality of this response remains to be understood. In this NASA NPP project, several candidate crop species such as lettuce, radish and tomato will be screened using stable isotopes to assess whether N preference changes in favor of ammonium in response to eCO2 and whether this remains true at super-elevated CO2 (seCO2), which is often experienced on the ISS. Photosynthetic and related measurements will help to disentangle the relationship between these responses and photosynthesis. This work will enable the development of optimized nutrient regimes for candidate crops in space and in future Lunar and Martian habitats and will pave the way for selection of crop varieties adapted to an eCO2 and/or seCO2 environment. Moreover, this research will further our understanding of plant responses to the eCO2 environment brought about by climate change, allowing the development of future-proof crops that will help to maintain food security. This NPP Fellowship is funded by NASA Space Biology.

space plant biology↗