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Filter Efficiency and Leak Testing of Returned ISS Bacterial Filter Elements After 2.5 Years of Continuous Operation

The atmosphere revitalization equipment aboard the International Space Station (ISS) and future deep space exploration vehicles provides the vital functions of maintaining a habitable environment for the crew as well as protecting the hardware from fouling by suspended particulate matter. Providing these functions are challenging in pressurized spacecraft cabins because no outside air ventilation is possible and a larger particulate load is imposed on the filtration system due to lack of sedimentation in reduced gravity conditions. The ISS Environmental Control and Life Support (ECLS) system architecture in the U.S. Segment uses a distributed particulate filtration approach consisting of traditional High-Efficiency Particulate Adsorption (HEPA) filters deployed at multiple locations in each module. These filters are referred to as Bacteria Filter Elements (BFEs). As more experience has been gained with ISS operations, the BFE service life, which was initially one year, has been extended to two to five years, dependent on the location in the U.S. Segment. In previous work we developed a test facility and test protocol for leak testing the ISS BFEs. For this work, we present results of leak testing a sample set of returned BFEs with a service life of 2.5 years, along with particulate removal efficiency and pressure drop measurements. The results can potentially be utilized by the ISS Program to ascertain whether the present replacement interval can be maintained or extended to balance the on-ground filter inventory with extension of the lifetime of ISS to 2024. These results can also provide meaningful guidance for particulate filter designs under consideration for future deep space exploration missions.

ISS Air Quality↗

Enhanced Characterization of Microorganisms in the Spacecraft Environment

Spacecraft such as the International Space Station (ISS) and the space shuttles are enclosed environments where crewmembers may spend long periods of time. Currently, crewmembers spend approximately a period of 6 months in the ISS. It is known that these prolonged stays in space may result in weakening of the immune system. Therefore, exposure to opportunistic pathogens or high concentrations of environmental microorganisms may compromise the health of the crew. The detection of biocontaminants in spacecraft environments utilizes culture-based methodology, omitting greater than 90% of all microorganisms including pathogens such as Legionella and Cryptosporidium. Culturable bacteria and fungi have been the only allergens studied; the more potent allergens, such as those from dust mites, have never been tested for in spacecraft environments. In addition, no attempts have been made to monitor microbial toxins in spacecrafts. The present study utilized quantitative polymerase chain reaction (QPCR) as a novel approach for monitoring microorganisms in the spacecraft environment. QPCR is a molecular biology technique that does not rely on the physiological state of the organisms for identification, thereby enabling detection of both culturable and non-culturable organisms. In this project, specific molecular primers and probes were utilized for the detection and quantitation of two fungi of concern in indoor environments, Aspergillus fumigatus and Stachybotrys chartarum. These organisms were selected because of the availability of PCR primers and probes, and to establish the sample processing and analysis methodology that may be employed with additional organisms. Purification methods and QPCR assays were optimized for the detection of these organisms in air, surface, and water; and sample processing and analysis protocols were developed. Preliminary validation of these protocols was conducted in the laboratory with air, surface, and water samples seeded with known concentrations of the target organisms. Additional studies were conducted with bulk materials (HEPA filter pleats and particulate found on the filter screen) obtained from the ISS.

Cruz, Patricia↗

Amateur Cleanrooms: Costs May Not Offset Benefits

Contamination and Coatings Branch During my career at NASA, I have encountered a variety of cleanroom systems. With many projects, cost pressures and lack of adequate facilities have forced the managers to resort to amateur cleanrooms to manufacture spacecraft and instruments. These rooms are usually spaces that have been used for other purposes that are converted to cleanroom, usually without the assistance of a contamination control specialist. Often, scientists and engineers are successful in converting an area for experimental use. However, when the area is used for production, countless difficulties are encountered. This paper will document some of the disasters that I have personally witnessed and offer some guidelines for contamination professionals to follow if you are called upon to assist in the development of new cleanrooms. Cleanroom come in all shapes and sizes from special purpose mini-environments (such as flow benches) to large, expansive production facilities. These areas may require a variety of unit operations to be carried out within a short range of each other. The design of the cleanroom should account for compatibilities of these operations to protect the product and personnel. The level of cleanliness has traditionally been associated with the method of ventilation. However, just because and =ea has HEPA filters and greater that 20 air changes per hour does not mean that it is a cleanroom. Airflow is extremely complex; the only way to properly design a cleanroom is through the use of a computer based model. In the aerospace industry, few engineered cleanrooms are modeled. Modeling has been perceived as expensive; however, modern programs and fast computers are changing perception. It is the lack of appreciation for how air flow and location within a cleanroom affects the product that causes most of the problems I have experienced. Currently, the rules defining the best air flow design practices are based on simplistic historical data that are often wrong. The performance of a cleanroom is defined by a set of complex interactions between the airflow, sources of contamination and heat, position of the air terminals and exhausts as well as the objects occupying the space in question. These subtleties are almost never appreciated in the setup of amateur cleanrooms (and sadly, in some engineered cleanroom as well). Experience with the room, measurement of air flows in the room, and black light inspections can be used to

Ramsey, W. Lawrence↗

Airborne Dust in Space Vehicles and Habitats

Airborne dust, suspended inside a space vehicle or in future celestial habitats, can present a serious threat to crew health if it is not controlled. During the Apollo missions to the moon, lunar dust brought inside the capsule caused eye irritation and breathing difficulty to the crew when they launched from the moon and re-acquired "microgravity." During Shuttle flights reactive and toxic dusts such as lithium hydroxide have created a risk to crew health, and fine particles from combustion events can be especially worrisome. Under nominal spaceflight conditions, airborne dusts and particles tend to be larger than on earth because of the absence of gravity settling. Aboard the ISS, dusts are effectively managed by HEPA filters, although floating dust in newly-arrived modules can be a nuisance. Future missions to the moon and to Mars will present additional challenges because of the possibility that external dust will enter the breathing atmosphere of the habitat and reach the crew's respiratory system. Testing with simulated lunar and Martian dust has shown that these materials are toxic when placed into the lungs of test animals. Defining and evaluating the physical and chemical properties of Martian dusts through robotic missions will challenge our ability to prepare better dust simulants and to determine the risk to crew health from exposure to such dusts.

James, John↗

The Influence of Microbiology on Spacecraft Design and Controls: A Historical Perspective of the Shuttle and International Space Station Programs

For over 40 years, NASA has been putting humans safely into space in part by minimizing microbial risks to crew members. Success of the program to minimize such risks has resulted from a combination of engineering and design controls as well as active monitoring of the crew, food, water, hardware, and spacecraft interior. The evolution of engineering and design controls is exemplified by the implementation of HEPA filters for air treatment, antimicrobial surface materials, and the disinfection regimen currently used on board the International Space Station. Data from spaceflight missions confirm the effectiveness of current measures; however, fluctuations in microbial concentrations and trends in contamination events suggest the need for continued diligence in monitoring and evaluation as well as further improvements in engineering systems. The knowledge of microbial controls and monitoring from assessments of past missions will be critical in driving the design of future spacecraft.

Castro, Victoria A.↗

Clean Assembly of Genesis Collector Canister for Flight: Lessons for Planetary Sample Return

Measurement of solar composition in the Genesis collectors requires not only high sensitivity but very low blanks; thus, very strict collector contamination minimization was required beginning with mission planning and continuing through hardware design, fabrication, assembly and testing. Genesis started with clean collectors and kept them clean inside of a canister. The mounting hardware and container for the clean collectors were designed to be cleanable, with access to all surfaces for cleaning. Major structural components were made of aluminum and cleaned with megasonically energized ultrapure water (UPW). The UPW purity was >18 M resistivity. Although aluminum is relatively difficult to clean, the Genesis protocol achieved level 25 and level 50 cleanliness on large structural parts; however, the experience suggests that surface treatments may be helpful on future missions. All cleaning was performed in an ISO Class 4 (Class 10) cleanroom immediately adjacent to an ISO Class 4 assembly room; thus, no plastic packaging was required for transport. Persons assembling the canister were totally enclosed in cleanroom suits with face shield and HEPA filter exhaust from suit. Interior canister materials, including fasteners, were installed, untouched by gloves, using tweezers and other stainless steel tools. Sealants/lubricants were not exposed inside the canister, but vented to the exterior and applied in extremely small amounts using special tools. The canister was closed in ISO Class 4, not to be opened until on station at Earth-Sun L1. Throughout the cleaning and assembly, coupons of reference materials that were cleaned at the same time as the flight hardware were archived for future reference and blanks. Likewise reference collectors were archived. Post-mission analysis of collectors has made use of these archived reference materials.

Allton, J. H.↗

Mold Species in Dust from the International Space Station Identified and Quantified by Mold Specific Quantitative PCR

Dust was collected over a period of several weeks in 2007 from various HEPA filters in the U.S. Laboratory Module of the International Space Station (ISS). The dust was returned on the Space Shuttle Atlantis, mixed, sieved, and the DNA was extracted. Using a DNA-based method called mold specific quantitative PCR (MSQPCR), 39 molds were measured in the dust. Opportunistic pathogens Aspergillus flavus and A. niger and toxin producers Penicillium chrysogenum and P. brevicompactum were found at relatively high concentrations (compared to U.S. homes). No cells of the opportunistic pathogens A. fumigatus, A. terreus, Fusarium solani or Candida albicans were detected.

Vesper, Stephen J.↗

Curating NASA's Past, Present, and Future Astromaterial Sample Collections

The Astromaterials Acquisition and Curation Office at NASA Johnson Space Center (hereafter JSC curation) is responsible for curating all of NASA's extraterrestrial samples. JSC presently curates 9 different astromaterials collections in seven different clean-room suites: (1) Apollo Samples (ISO (International Standards Organization) class 6 + 7); (2) Antarctic Meteorites (ISO 6 + 7); (3) Cosmic Dust Particles (ISO 5); (4) Microparticle Impact Collection (ISO 7; formerly called Space-Exposed Hardware); (5) Genesis Solar Wind Atoms (ISO 4); (6) Stardust Comet Particles (ISO 5); (7) Stardust Interstellar Particles (ISO 5); (8) Hayabusa Asteroid Particles (ISO 5); (9) OSIRIS-REx Spacecraft Coupons and Witness Plates (ISO 7). Additional cleanrooms are currently being planned to house samples from two new collections, Hayabusa 2 (2021) and OSIRIS-REx (2023). In addition to the labs that house the samples, we maintain a wide variety of infra-structure facilities required to support the clean rooms: HEPA-filtered air-handling systems, ultrapure dry gaseous nitrogen systems, an ultrapure water system, and cleaning facilities to provide clean tools and equipment for the labs. We also have sample preparation facilities for making thin sections, microtome sections, and even focused ion-beam sections. We routinely monitor the cleanliness of our clean rooms and infrastructure systems, including measurements of inorganic or organic contamination, weekly airborne particle counts, compositional and isotopic monitoring of liquid N2 deliveries, and daily UPW system monitoring. In addition to the physical maintenance of the samples, we track within our databases the current and ever changing characteristics (weight, location, etc.) of more than 250,000 individually numbered samples across our various collections, as well as more than 100,000 images, and countless "analog" records that record the sample processing records of each individual sample. JSC Curation is co-located with JSC's Astromaterials Research Office, which houses a world-class suite of analytical instrumentation and scientists. We leverage these labs and personnel to better curate the samples. Part of the cu-ration process is planning for the future, and we refer to these planning efforts as "advanced curation". Advanced Curation is tasked with developing procedures, technology, and data sets necessary for curating new types of collections as envi-sioned by NASA exploration goals. We are (and have been) planning for future cu-ration, including cold curation, extended curation of ices and volatiles, curation of samples with special chemical considerations such as perchlorate-rich samples, and curation of organically- and biologically-sensitive samples.

Zeigler, R. A.↗

Curating NASA's Past, Present, and Future Extraterrestrial Sample Collections

As codified in NASA Policy Directive 7100.10F, the Astromaterials Acquisition and Curation Office at NASA Johnson Space Center (hereafter JSC Curation) is charged with curation of all extraterrestrial material under NASA control, including future NASA missions. JSC Curation curates all or part of nine astromaterial collections in seven clean room suites: (1) Apollo Samples (1969; ISO 6-7), (2) Luna Samples (from USSR; 1972; ISO 7), (3) Antarctic Meteorites (1976; ISO 7), (4) Cosmic Dust (1981; ISO 5), (5) Microparticle Impact Collection (formerly called Space Exposed Hardware; 1985; ISO 5), (6) Genesis Solar Wind Atoms (2004; ISO 4); (7) Stardust Comet Particles (2006; ISO 5), (8) Stardust Interstellar Particles (2006; ISO 5), (9) Hayabusa Asteroid Particles (from JAXA; 2010; ISO 5). In addition to the labs that house the samples, we have installed and maintained a wide variety of facilities and infrastructure required to support the clean-rooms: more than 10 different HEPA-filtered air-handling systems, ultrapure dry gaseous nitrogen systems, an ultrapure water system (UPW) and cleaning facilities to provide clean tools and equipment for the labs. We also have sample preparation facilities for making thin sections, microtome sections, and even focused ion-beam (FIB) sections to meet the research requirements of scientists. To ensure that we are keeping the samples as pristine as possible, we routinely monitor the cleanliness of our clean rooms and infrastructure systems. This monitoring includes: daily monitoring of the quality of our UPW, weekly airborne particle counts in the labs, monthly monitoring of the stable isotope composition of the gaseous N2 system, and annual measurements of inorganic or organic contamination in processing cabinets. We track within our databases the current and ever-changing characteristics of more than 250,000 individual samples across our various collections (including the 19,141 samples on loan to 433 Principal Investigators in 24 countries). The next sample return missions that NASA will participate in are Hayabusa2 and OSIRIS-REx (Origins Spectral Interpretation Resource Identification Security - Regolith Explorer). The designs for a new state-of-the-art suite of clean rooms to house these samples at JSC have been finalized. This includes separate ISO class 5 clean rooms to house each collection, a common ISO class 7 area for general use, an ISO class 7 microtome laboratory, and a separate thin section lab. Additionally, a new cleaning facility is being designed and procedures developed that will allow for enhanced cleaning of cabinets and tools in an inorganically, organically, and biologically clean manner. We are also designing a large multi-purpose Advanced Curation laboratory that will allow us to develop the techniques necessary to fully support the Hayabusa2 and OSIRIS-REx missions, as well as future possible sample return missions (e.g., Lunar Polar Volatiles, Mars, Comet Surface). A micro-CT (micro Computed Tomography) laboratory dedicated to the study of astromaterials has come online within JSC Curation, and we plan to add additional facilities that will enable non-destructive (or minimally-destructive) analyses of astromaterials in the near future (e.g., micro-XRF (micro X-Ray Fluorescence), confocal imaging Raman Spectroscopy). These facilities will be available to: (1) develop sample handling and storage techniques for future sample return missions, (2) be utilized by PET (Positron Emission Tomography) for future sample return missions, (3) for retroactive PET-style analyses of our existing collections, and (4) for periodic assessments of the existing sample collections.

Zeigler, Ryan A.↗

Regolith Particle Erosion of Material in Aerospace Environments

This paper studies the effect of exposing thermal control S13GP:6N/LO-I white paint, Kapton flex cable, fiber optic cable, HEPA filter, and M55J graphite composite to high-velocity regolith environment that spacecraft landing on Mars are commonly exposed to. Due to the similarity between the Mars 2020 Rover design and Mars Science Laboratory design, it is expected that the Mars 2020 rover will be exposed to a similar high-speed regolith environment that the Mars Science Laboratory was exposed to. This environment is replicated to test the survivability of susceptible materials. The testing is performed at the University of Dayton Research Institute in Dayton, Ohio. The experiments expose different materials to basaltic–like particles ranging in size from approximately 40 μm to 2 cm, at velocities ranging from 19 m/s to 250 m/s, with varied particle fluxes (measured in mg/cm2). Depending on the size of the particle used, the particles can either embed in or erode the material. Posttest analysis shows that all materials tested will survive the expected environment observed during the Mars 2020 landing event. Some materials are tested to failure in order to better characterize material response. Materials that fail in some test scenarios include the paint, fiber optic cable, and the graphite composite. After being exposed to regolith, the α/ε ratio of the paint increased by ~37% due to particles embedding in the paint. Darkening of the paint can negatively affect thermal control of the rover. With high particle mass fluxes, the paint eventually degraded enough to expose the aluminum substrate. When impacted by a 1.5 cm particle traveling at 20 m/s, the fiber optic cable did not sever, but the impact did cause the cable to deform enough to crack the glass, which resulted in a significant increase in attenuation, rendering the cable unable to transmit data. The graphite composite also failed when exposed to high particle fluxes. All of the observed failures occurred for test cases above the expected landing environment with significant margin. Tests performed beyond the requirements help characterize how well these materials will survive in even more extreme environments for future missions.

Abid, Mohamed↗

Center for Isotope Cosmochemistry and Geochronology at NASA Johnson Space Center

Ten years in the making the Center for Isotope Cosmochemistry and Geochronology (CICG) at NASA Johnson Space Center is designed to be a cooperative analytical facility where novel techniques are developed and performed. Our team works collaboratively to operate this integrated lab suite comprising sample preparation, metal-free clean chemistry, and mass spectrometers to measure a wide variety of elements and isotopes in astromaterials. Contamination control is enacted throughout by a positive pressure cascade of HEPA filtered air, weekly surface cleaning and particle counting. Astromaterials processing is performed with application-specific mortar and pestles, heavy liquid separation, a Frantz magnetic separator, as well as hand-picking using optical microscopes in laminar flow boxes. Microsampling is also carried out using a New Wave MicroMill. Elements for isotopic analysis are isolated from acid-digested samples using ion-exchange chromatography. Cross-contamination is minimized with dedicated laminar flow boxes and labware for different purification protocols. Instrumentation includes a ThermoFisher Element XR inductively coupled plasma mass spectrometer (ICP-MS) for quantification of elemental concentrations in dissolved sample solutions. For in situ analysis, a Teledyne Photon Machines Analyte Excite+ laser ablation system is coupled to the Element XR. The ThermoFisher Triton thermal ionization mass spectrometer (TIMS) is used to measure the isotopic composition of elements such as CA, K, Cr, Rb, Sr, Sm, Nd at high precision in processed samples. For a more diverse range of isotopic analyses, CICG houses a Nu Sapphire 1700 multi-collector inductively coupled plasma mass spectrometer (MC-ICP-MS), which is a large geometry instrument with a multi-pole collision cell for interference removal. An Applied Spectra Instruments J200 femto-second laser is planned for install this Fall and will be coupled to the Nu Sapphire 1700. NASA’s Internal Scientist Funding Model (ISFM)supports this facility. Advanced analytical geochemistry techniques are applied to targeted astromaterials to address important problems in planetary sciences, with a longer-term goal of preparing for samples collected and returned by space missions. CICG is open to collaborations on projects that utilize the facility’s unique capabilities and are within its research scope. We are particularly eager to support and share expertise with the next generation of planetary scientists.

Justin I Simon↗

An Overview of Contamination Control for the James Webb Space Telescope Launch Campaign

The James Webb Space Telescope (JWST) is a large, infrared space telescope operating at Lagrange point 2. JWST is a joint effort between NASA, ESA, and CSA and was launched from the Centre Spatial Guyanais (CSG) on an Ariane 5 rocket in December 2021. The three-month launch campaign utilized enhanced contamination controls to meet JWST’s strict cleanliness requirements. Prior to launch, JWST was permitted to only be exposed to ISO Class 7 cleanrooms, whereas the processing facilities at CSG are ISO Class 8. NASA, ESA, Arianespace, and CNES implemented temporary upgrades to the nominal contamination control operations for the launch campaign unique to JWST, including the use of vetted, portable High Efficiency Particulate Air (HEPA) filter walls, pre-entrance cleanliness acceptance surveys of each facility and the intra-plant transporter, tightened cleanroom protocols, upgraded garmenting and laundering techniques, cleaning of Self-Contained Atmospheric Protection Ensemble (SCAPE) suits, increased maintenance, staffed pre-cleaning stations, adaptation of the house purge network, and a contamination control enclosure atop the Ariane 5 launcher prior to fairing encapsulation. The Ariane 5 fairing interior and Vehicle Equipment Bay membrane also received multiple cleanings, detailed inspections, and verification sampling to achieve necessary cleanliness levels. The fairing itself was specially sealed to protect the inner environment with just a small, door porthole accessible via diving board for final closeout of the purge interface. All of these enhancements together allowed JWST to meet its contamination requirements for launch, ensuring successful post-separation deployments and mission science.

Contamination Control↗

Overview of Contamination Control for the James Webb Space Telescope Launch Campaign

The James Webb Space Telescope (JWST) is a large, infrared space telescope operating at Lagrange point 2. JWST is a joint effort between NASA, ESA, and CSA and was launched from the Centre Spatial Guyanais (CSG) on an Ariane 5 rocket in December 2021. The three-month launch campaign utilized enhanced contamination controls to meet JWST’s strict cleanliness requirements. Prior to launch, JWST was permitted to only be exposed to ISO Class 7 cleanrooms, whereas the processing facilities at CSG are ISO Class 8. NASA, ESA, Arianespace, and CNES implemented temporary upgrades to the nominal contamination control operations for the launch campaign unique to JWST, including the use of vetted, portable High Efficiency Particulate Air (HEPA) filter walls, pre-entrance cleanliness acceptance surveys of each facility and the intra-plant transporter, tightened cleanroom protocols, upgraded garmenting and laundering techniques, cleaning of Self-Contained Atmospheric Protection Ensemble (SCAPE) suits, increased maintenance, staffed pre-cleaning stations, adaptation of the house purge network, and a contamination control enclosure atop the Ariane 5 launcher prior to fairing encapsulation. The Ariane 5 fairing interior and Vehicle Equipment Bay membrane also received multiple cleanings, detailed inspections, and verification sampling to achieve necessary cleanliness levels. The fairing itself was specially sealed to protect the inner environment with just a small, doored porthole accessible via diving board for final closeout of the purge interface. All these enhancements together allowed JWST to meet its contamination requirements for launch, ensuring successful post-separation deployments and mission science.

James Webb Space Telescope↗

Overview of Contamination Control for the James Webb Space Telescope Launch Campaign

The James Webb Space Telescope (JWST) is a large, infrared space telescope operating at Lagrange point 2. JWST is a joint effort between NASA, ESA, and CSA and was launched from the Centre Spatial Guyanais (CSG) on an Ariane 5 rocket in December 2021. The three-month launch campaign utilized enhanced contamination controls to meet JWST’s strict cleanliness requirements. Prior to launch, JWST was permitted to only be exposed to ISO Class 7 cleanrooms, whereas the processing facilities at CSG are ISO Class 8. NASA, ESA, Arianespace, and CNES implemented temporary upgrades to the nominal contamination control operations for the launch campaign unique to JWST, including the use of vetted, portable High Efficiency Particulate Air (HEPA) filter walls, pre-entrance cleanliness acceptance surveys of each facility and the intra-plant transporter, tightened cleanroom protocols, upgraded garmenting and laundering techniques, cleaning of Self-Contained Atmospheric Protection Ensemble (SCAPE) suits, increased maintenance, staffed pre-cleaning stations, adaptation of the house purge network, and a contamination control enclosure atop the Ariane 5 launcher prior to fairing encapsulation. The Ariane 5 fairing interior and Vehicle Equipment Bay membrane also received multiple cleanings, detailed inspections, and verification sampling to achieve necessary cleanliness levels. The fairing itself was specially sealed to protect the inner environment with just a small, doored porthole accessible via diving board for final closeout of the purge interface. All these enhancements together allowed JWST to meet its contamination requirements for launch, ensuring successful post-separation deployments and mission science.

James Webb Space Telescope, launch, contamination,↗

Predicting contamination accumulation in facilities with limited data

In preparation for the James Webb Space Telescope (JWST) launch at Centre Spatial Guyanias (CSG) in French Guiana, particulate contamination accumulation predictions were necessary for each facility in which the hardware would be exposed because the Telescope would be uncovered in each of the facilities and had strict particulate requirements. These included facilities for final integration and testing, fueling, transportation and encapsulation. Minimal heritage data existed from CSG and previous launch campaigns to use as a basis for contamination predictions. Data from the Automatic Transfer Vehicle and Herschel & Planck launch campaigns were used in conjunction with facility monitoring data provided by ESA and data collected during JWST working group visits to CSG. These campaigns were conducted at varying cleanliness levels that were typically less stringent than JWST requirements. Each facility was evaluated using the data provided and likely performance improvement with the addition of High Efficiency Particulate Air filter (HEPA) banks operating when possible. Once the launch campaign was completed, the predicted fallout was compared with actual data collected throughout the campaign. Based on the actual measurements, JWST’s primary and secondary mirrors turned out to be much cleaner than what was predicted.

James Webb Space Telescope, contamination, cleanro↗

Predicting contamination accumulation in facilities with limited data

"In preparation for the James Webb Space Telescope (JWST) launch at Centre Spatial Guyanias (CSG) in French Guiana, particulate contamination accumulation predictions were necessary for each facility in which the hardware would be exposed because the Telescope would be uncovered in each of the facilities and had strict particulate requirements. These included facilities for final integration and testing, fueling, transportation and encapsulation. Minimal heritage data existed from CSG and previous launch campaigns to use as a basis for contamination predictions. Data from the Automatic Transfer Vehicle and Herschel & Planck launch campaigns were used in conjunction with facility monitoring data provided by ESA and data collected during JWST working group visits to CSG. These campaigns were conducted at varying cleanliness levels that were typically less stringent than JWST requirements. Each facility was evaluated using the data provided and likely performance improvement with the addition of High Efficiency Particulate Air filter (HEPA) banks operating when possible. Once the launch campaign was completed, the predicted fallout was compared with actual data collected throughout the campaign. Based on the actual measurements, JWST’s primary and secondary mirrors are likely much cleaner than was expected with the predicted fallout in each facility. "

James Webb Space Telescope, contamination, cleanro↗

Mitigating Microbial Contamination Risks in CO 2 Removal Systems for Long-Duration Space Missions

Crewed missions to Mars and beyond pose new challenges for the crew and the technological solutions they depend upon. Crucial considerations in designing the next generation of Environmental Control and Life Support Systems (ECLSS) include enhanced reliability and autonomy, given the challenges of resupply and resource recovery in remote missions. One significant challenge faced in various sectors of the International Space Station and ECLSS is the phenomena of microbial growth, particularly biofilm formation. As such, microbial growth is a critical consideration in the design phase of new ECLSS systems. Two novel CO2 removal systems—Liquid-Amines CO2 Removal (LACR) and Cold-Surface CO2 Deposition (CDep)—are currently under development at the NASA Ames Research Center to meet the needs of extended space travel. In this presentation, the authors aim to make a risk assessment of microbial growth in LACR and CDep through: 1) a comprehensive literature review to identify LACR and CDep components most vulnerable to microbial contamination and 2) wet lab testing commencing with a subscale test of the most susceptible component of CDep. The literature review revealed that the hollow-fiber Liqui-Cel membrane employed in CDep exhibits the highest susceptibility to microbial growth. Our wet lab tests corroborated these findings, as airborne microbes introduced into the membrane led to considerable biomass accumulation. These results indicate a significant risk of microbial invasion into the membranes. To prevent microbial growth in CDep or other CO2 removal systems, proper ECLSS arrangement is essential—a CO2 removal system should remain behind the charcoal-HEPA filters, condensing heat exchanger, and Trace Contaminant Control System. In summary, anticipating potential microbial interactions within ECLSS for remote crewed missions is vital to mission success. It should, therefore, be included as one of the many factors when designing future ECLSS systems to perform reliably and consistently to support astronaut life.

A. Nicolas Whitlock↗