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Liu, W. T.

Publications and source records attributed to Liu, W. T..

At least 37 records · Page 2

Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach

The utility of a high-density oligonucleotide microarray (microchip) for identifying strains of five closely related bacilli (Bacillus anthracis, Bacillus cereus, Bacillus mycoides, Bacillus medusa and Bacillus subtilis) was demonstrated using an approach that compares the non-equilibrium dissociation rates ('melting curves') of all probe-target duplexes simultaneously. For this study, a hierarchical set of 30 oligonucleotide probes targeting the 16S ribosomal RNA of these bacilli at multiple levels of specificity (approximate taxonomic ranks of domain, kingdom, order, genus and species) was designed and immobilized in a high-density matrix of gel pads on a glass slide. Reproducible melting curves for probes with different levels of specificity were obtained using an optimized salt concentration. Clear discrimination between perfect match (PM) and mismatch (MM) duplexes was achieved. By normalizing the signals to an internal standard (a universal probe), a more than twofold discrimination (> 2.4x) was achieved between PM and 1-MM duplexes at the dissociation temperature at which 50% of the probe-target duplexes remained intact. This provided excellent differentiation among representatives of different Bacillus species, both individually and in mixtures of two or three. The overall pattern of hybridization derived from this hierarchical probe set also provided a clear 'chip fingerprint' for each of these closely related Bacillus species.

Non-NASA Center↗

Asian flooding induced by marine winds

The methodologies of estimating both ocean surface winds vectors over the ocean and flooding index over land by spaceborne scatterometers are described.

flood winds remote sensing↗

A neural network to retrieve the mesoscale instantaneous latent heat flux over oceans from SSM/I observations

The turbulent latent and sensible heat fluxes are necessary to study heat budget of the upper ocean or initialize ocean general circulation models. In order to retrieve the latent heat flux from satellite observations authors mostly use a bulk approximation of the flux whose parameters are derived from different instrument. In this paper, an approach based on artificial neural networks is proposed and compared to the bulk method on a global data set and 3 local data sets.

SSM/I latent heat flux air-sea interactions↗