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Visualization of DNA molecules in time during electrophoresis

For several years individual DNA molecules have been observed and photographed during agarose gel electrophoresis. The DNA molecule is clearly the largest molecule known. Nevertheless, the largest molecule is still too small to be seen using a microscope. A technique developed by Morikawa and Yanagida has made it possible to visualize individual DNA molecules. When these long molecules are labeled with appropriate fluorescence dyes and observed under a fluorescence microscope, although it is not possible to directly visualize the local ultrastructure of the molecules, yet because they are long light emitting chains, their microscopic dynamical behavior can be observed. This visualization works in the same principle that enables one to observe a star through a telescope because it emits light against a dark background. The dynamics of individual DNA molecules migrating through agarose matrix during electrophoresis have been described by Smith et al. (1989), Schwartz and Koval (1989), and Bustamante et al. (1990). DNA molecules during agarose gel electrophoresis advance lengthwise thorough the gel in an extended configuration. They display an extension-contraction motion and tend to bunch up in their leading ends as the 'heads' find new pores through the gel. From time to time they get hooked on obstacles in the gel to form U-shaped configurations before they resume their linear configuration.

Lubega, Seth↗

Low NO.sub.x combustor

A combustor includes a dome assembly having radially outer and inner liners joined thereto and defining therebetween a combustion zone. The dome assembly includes at least one annular dome having a pair of axially extending first flanges between which are disposed a plurality of circumferentially spaced apart carburetors for discharging a fuel/air mixture into the combustion zone for generating combustion gases. An annular heat shield includes a pair of axially extending legs integrally joined to a radially extending face in a generally U-shaped configuration, with the face including a plurality of circumferentially spaced apart ports disposed concentrically with perspective ones of the carburetors for allowing the fuel/air mixture to be discharged therefrom through the heat shield. At least one of the heat shield legs includes a plurality of circumferentially spaced apart mounting holes disposed adjacent to a respective one of the dome flanges, and a plurality of mounting pins are fixedly joined to the dome flange and extend radially through respective ones of the mounting holes without interference therewith for allowing unrestrained thermal movement between the heat shield and the dome while supporting the heat shield against axial pressure loads thereon. In a preferred embodiment, the dome assembly includes three domes having respective ones of the heat shield, and respective baffles are spaced from the heat shields for providing impingement cooling thereof.

Halila, Ely E.↗

Modeling instrumented test articles for Plasmatron X aerothermal environment

This work discusses the ablative response of thermal protection system materials when exposed to aerothermal environments. It focuses on modeling instrumented test article assemblies tested in the Plasmatron X inductively coupled plasma wind tunnel. For this purpose, as the samples are mounted to a water-cooled arm using numerous assembly components and are embedded with thermocouples for in-depth temperature measurements, the variation in ablative response of the samples as a result of these components is studied. The aerothermal boundary conditions for Plasmatron X are obtained for hypersonic flight-relevant conditions using the multi-physics aerothermal framework built at the University of Illinois at Urbana-Champaign. For simulating the material response, a material response code, Porous Material Analysis Toolbox based on OpenFOAM (PATO) is used. In PATO, numerous configurations and materials of thermocouples were studied. The optimal configuration for obtaining temperature measurements with minimal error using embedded thermocouples was found to be the U-shaped Type S, 30 American wire gauge thermocouple configuration.

Thermal Protection Systems↗

Modeling instrumented test articles for Plasmatron X aerothermal environment

This work discusses the ablative response of thermal protection system materials when exposed to aerothermal environments. It focuses on modeling instrumented test article assemblies tested in the Plasmatron X inductively coupled plasma wind tunnel. For this purpose, as the samples are mounted to a water-cooled arm using numerous assembly components and are embedded with thermocouples for in-depth temperature measurements, the variation in ablative response of the samples as a result of these components is studied. The aerothermal boundary conditions for Plasmatron X are obtained for hypersonic flight-relevant conditions using the multi-physics aerothermal framework built at the University of Illinois at Urbana-Champaign. For simulating the material response, a material response code, Porous Material Analysis Toolbox based on OpenFOAM (PATO) is used. In PATO, numerous configurations and materials of thermocouples were studied. The optimal configuration for obtaining temperature measurements with minimal error using embedded thermocouples was found to be the U-shaped Type S, 30 American wire gauge thermocouple configuration.

Thermal Protection Systems↗

Investigation of Selectively-Reinforced Metallic Lugs

An investigation of the effects of material and geometric variables on the response of U-shaped band-reinforced metallic lugs was performed. Variables studied were reinforcement, adhesive and metallic lug mechanical properties, hole diameter, reinforcement and adhesive thickness, and the distance from the hole s center to the end of the lug. Generally, U-shaped band reinforced lugs exhibited superior performance than non-reinforced lugs, that is higher load at the conventional lug design criteria of four percent hole elongation. Depending upon the reinforcement configuration the increase in load may be negligible to 15 or 20 percent. U-shaped band reinforcement increases lug load carrying capability primarily through two mechanisms; increasing the slope of the response curve after the initial knee and restraining overall deformation of the metallic portion of the lug facilitating increased yielding of metallic material between the hole and the edge of the metallic portion of the lug.

Farley, Gary L.↗

The development of large diameter, high pressure, cryogenic radial static seals

Two configurations of radial static seals are developed for high-pressure 773 kg per sq cm cryogenic applications: a U-shaped fluorocarbon seal and a delta-shaped PTFE seal. The U-seal is a common commercial design consisting of a spring-loaded cup-shaped jacket; the basic principle is that the soft plastic jacket provides the interface between the piston and the bore, the jacket being held in place by a metal spring. The delta seal is based on the principle that the soft plastic delta cross section furnishes the interface at the metal face and circumferential face of the gland and cylinder. Test results indicate that both the plastic U-seal and the delta seal designs are sufficiently flexible to accommodate assembly in bore or piston grooves. Of these two configurations, the delta seal is found to be superior as it meets all the design requirements for proof pressure testing the turbopump components of the main engine in the Space Shuttle.

Burr, M. E.↗

Progress Towards Fuselage Drag Reduction via Active Flow Control: A Combined CFD and Experimental Effort

A combined computational and experimental effort has been undertaken to study fuselage drag reduction on a generic, non-proprietary rotorcraft fuselage by the application of active ow control. Fuselage drag reduction is an area of research interest to both the United States and France and this area is being worked collaboratively as a task under the United States/France Memorandum of Agreement on Helicopter Aeromechanics. In the first half of this task, emphasis is placed on the US generic fuselage, the ROBIN-mod7, with the experimental work being conducted on the US side and complementary US and French CFD analysis of the baseline and controlled cases. Fuselage simulations were made using Reynolds-averaged Navier-Stokes ow solvers and with multiple turbulence models. Comparisons were made to experimental data for numerical simulations of the isolated fuselage and for the fuselage as installed in the tunnel, which includes modeling of the tunnel contraction, walls, and support fairing. The numerical simulations show that comparisons to the experimental data are in good agreement when the tunnel and model support are included. The isolated fuselage simulations compare well to each other, however, there is a positive shift in the centerline pressure when compared to the experiment. The computed flow separation locations on the rear ramp region had only slight differences with and without the tunnel walls and model support. For the simulations, the flow control slots were placed at several locations around the flow separation lines as a series of eight slots that formed a nearly continuous U-shape. Results from the numerical simulations resulted in an estimated 35% fuselage drag reduction from a steady blowing flow control configuration and a 26% drag reduction for unsteady zero-net-mass flow control configuration. Simulations with steady blowing show a delayed flow separation at the rear ramp of the fuselage that increases the surface pressure acting on the ramp, thus decreasing the overall fuselage pressure drag.

Schaeffler, Norman W.↗

Pressure-Energized Seal Rings to Better Withstand Flows

Pressure-energized seal rings intended to withstand flows better than do conventional pressure-energized seal rings have been conceived. The concept applies, more specifically, to seal rings used on some valve stems, pistons, and the like. A conventional pressure-energized seal ring generally has a U-shaped cross section and consists of an elastomer or other suitable polymer with an embedded metal energizing spring (see Figure 1). The working fluid from the high-pressure side that one seeks to seal is allowed into the U-shaped cavity, so that the pressure pushes the sides of the seal ring tighter against the gland and body sealing surfaces, thereby increasing the degree of sealing. Unfortunately, when the seal ring is exposed to flow of the working fluid, under some conditions, the flow grabs the lip of the U-shaped cross section and ejects or deforms the seal ring so that, thereafter, a proper seal is not obtained. Figure 2 depicts one of several alternative seal rings according to the present concept. One element of the concept is to modify the U-shaped cross section from that of the corresponding conventional seal ring to eliminate the exposed lip and prevent entry of the working fluid into the U-shaped cavity. Unlike in the conventional seal, pressurized fluid would not push the seal ring directly against the both gland and body sealing surfaces. Instead, the pressure would directly push the seal ring against a gland sealing surface only. In so doing, the pressure would squash the seal ring into a smaller volume bounded by the gland and body sealing surfaces, and would thereby indirectly press the seal ring more tightly against the body sealing surface. To enhance the desired squashing deformation, a spring having an approximately parallelogram cross section would be embedded in the modified U-shaped cavity. As the pressure pushed two corners of the approximate parallelogram closer together along the axis of the seal ring, the other two corners of the approximate parallelogram would be pushed farther apart along a radius of the ring, thereby causing the polymeric ring material to push radially harder against the body sealing surface. From the radially innermost corner of the approximate parallelogram, the spring material would extend radially, then axially into recesses in the seal gland. These extensions would help to restrain the seal ring against ejection. A seat retainer would hold the sealing ring in the gland and form a mechanical compression seal to prevent or at least reduce leakage of pressurized fluid into the cavity behind the seal. However, because there would likely be a little leakage, the cavity behind the seal should be vented to the low pressure side to prevent buildup of pressure in the cavity over time; otherwise, the built-up pressure could cause ejection of the seal ring when the pressure on the high-pressure side was reduced. Polymeric seal-ring materials may not be able to withstand working conditions in applications that involve abrasive and/or hot working fluids. For such applications, all-metal seal rings may be preferred. The bottom part of Figure 2 shows one example of an alternative gland configuration with an all-metal seal ring.

Farner, Bruce↗