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The aim of this study is to investigate the effect of the cell culture conditions of three-dimensional polymer scaffolds seeded with rat marrow stromal cells (MSCs) cultured in different bioreactors concerning the ability of these cells to proliferate, differentiate towards the osteoblastic lineage, and generate mineralized extracellular matrix. MSCs harvested from male Sprague-Dawley rats were culture expanded, seeded on three-dimensional porous 75:25 poly(D,L-lactic-co-glycolic acid) biodegradable scaffolds, and cultured for 21 days under static conditions or in two model bioreactors (a spinner flask and a rotating wall vessel) that enhance mixing of the media and provide better nutrient transport to the seeded cells. The spinner flask culture demonstrated a 60% enhanced proliferation at the end of the first week when compared to static culture. On day 14, all cell/polymer constructs exhibited their maximum alkaline phosphatase activity (AP). Cell/polymer constructs cultured in the spinner flask had 2.4 times higher AP activity than constructs cultured under static conditions on day 14. The total osteocalcin (OC) secretion in the spinner flask culture was 3.5 times higher than the static culture, with a peak OC secretion occurring on day 18. No considerable AP activity and OC secretion were detected in the rotating wall vessel culture throughout the 21-day culture period. The spinner flask culture had the highest calcium content at day 14. On day 21, the calcium deposition in the spinner flask culture was 6.6 times higher than the static cultured constructs and over 30 times higher than the rotating wall vessel culture. Histological sections showed concentration of cells and mineralization at the exterior of the foams at day 21. This phenomenon may arise from the potential existence of nutrient concentration gradients at the interior of the scaffolds. The better mixing provided in the spinner flask, external to the outer surface of the scaffolds, may explain the accelerated proliferation and differentiation of marrow stromal osteoblasts, and the localization of the enhanced mineralization on the external surface of the scaffolds. Copyright 2002 Wiley Periodicals, Inc.
Convective heat-transfer coefficients in dry air were obtained for an ellipsoidal spinner of 30-inch maximum diameter for both stationary and rotating operation over a range of conditions including airspeeds up to 275 miles per hour, rotational speeds up to 1200 rpm, and angles of attack of zero and 40 The results are presented in terms of Nusselt numbers, Reynolds numbers, and convective heat-transfer coefficients. The studies included both uniform heating densities over the spinner and uniform surface temperatures.. In general, the results showed that rotation will increase the convective heat transfer from a spinner, especially in the turbulent-flow regions. Rotation of the spinner at 1200 rpm and at a free-stream velocity of 275 miles per hour increased the Nusselt number parameter in the turbulent-flow region by 32 percent over that obtained with a stationary spinner; whereas in the nose region, where the flow was laminar, an increase of only 18 percent was observed. Transition from laminar to turbulent flow occurred over a large range of Reynolds numbers primarily because of surface roughness of the spinner. Operation at an angle of attack of 40 had only small effects on the local convective heat transfer for the model studied.
Report presents the results of an investigation conducted in the Langley propeller research tunnel to study cowling-spinner combinations based on the NACA 1-series nose inlets and to obtain systematic design data for one family of approximately ellipsoidal spinners. In the main part of the investigation, 11 of the related spinners were tested in various combinations with 9 NACA open-nose cowlings, which were also tested without spinners. The effects of location and shape of the spinner, shape of the inner surface of the cowling lip, and operation of a propeller having approximately oval shanks were investigated briefly. In addition, a study was conducted to determine the correct procedure for extrapolating design conditions determined from the low-speed test data to the design conditions at the actual flight Mach number.
Wind-tunnel tests of several propeller, cuff, and spinner combinations were conducted in the 20 foot propeller-research tunnel. Three propellers, which ranged in diameter from 8.4 to 11.25 feet, were tested at the front end of a streamline body incorporating spinners of two diameters. The tests covered a blade angle range from 20 deg to 65 deg. The effect of spinner diameter and propeller cuffs on the characteristics of one propeller was determined. Test were also conducted using a propeller which incorporated aerodynamically good shank sections and using one which incorporated the NACA 16 series sections for the outer 20 percent of the blades. Compressibility effects were not measured, owing to the low testing speeds. The results indicated that a conventional propeller was slightly more efficient when tested in conjunction with a 28 inch diameter spinner than with a 23 inch spinner, and that cuffs increased the efficiency as well as the power absorption characteristics. A propeller having good aerodynamic shanks was found to be definitely superior from the efficiency standpoint to a conventional round-shank propeller with or without cuffs; this propeller would probably be considered structurally impracticable, however. The propeller incorporating the NACA 16 series sections at the tims were found to have a slightly higher efficiency than a conventional propeller; the take-off characteristics appeared to be equally good. The effects noted above probably would be accentuated at helical speeds at which compressibility effects would enter.
Wind-tunnel tests are reported of five 3-blade 10-foot propellers operating in front of a radial and a liquid-cooled engine nacelle. The range of blade angles investigated extended from 15 degrees to 45 degrees. Two spinners were tested in conjunction with the liquid-cooled engine nacelle. Comparisons are made between propellers having different blade-shank shapes, blades of different thickness, and different airfoil sections. The results show that propellers operating in front of the liquid-cooled engine nacelle had higher take-off efficiencies than when operating in front of the radial engine nacelle; the peak efficiency was higher only when spinners were employed. One spinner increased the propulsive efficiency of the liquid-cooled unit 6 percent for the highest blade-angle setting investigated and less for lower blade angles. The propeller having airfoil sections extending into the hub was superior to one having round blade shanks. The thick propeller having a Clark y section had a higher take-off efficiency than the thinner one, but its maximum efficiency was possibly lower. Of the three blade sections tested, Clark y, R.A.F. 6, and NACA 2400-34, the Clark y was superior for the high-speed condition, but the R.A.F. 6 excelled for the take-off condition.
A method for calculating the flow fields of axially symmetric bodies from their pressure distributions is reported in NACA RM No. L8I17. In order to facilitate application of this method to the important case of the cowling-spinner combination, for use in the design of propellers, the present paper presents static-pressure distributions on the tops of 79 high-critical-speed NACA 1-series cowling-spinner combinations over wide ranges of inlet-velocity ratio at angles of attack of 0 degrees, 2 degrees, 4 degrees, and 6 degrees. Static-pressure distributions around the nose sections of several cowlings are given in greater detail to aid in estimating the pressures near the stagnation points and to show the effect of changes in the internal lip shape. The effects of the operation of a typical propeller on the surface pressures on the cowling are shown for one configuration. The pressure distributions over the nine NACA 1-series nose inlets used as the basic components of these combinations are also presented ro supplement the existing open-nose-cowling data of NACA ACR No. L5F30a which are applicable to the case of the rotating cowling.
A flight investigation has been made of the surface pressure distribution and the flow field around a dummy, nonrotating, elliptical spinner over a Mach number range from 0.65 to 0.95, which corresponds to a Reynolds number range from about 1.6 x 10(exp 6) per foot to about 3.9 x 10(exp 6) per foot. The results showed that free-stream conditions were approximated from about 15 to 90 percent of the spinner length, but the local Mach number in the propeller plane varied from about 5 percent less than free stream at a Mach number of 0.65 to about 10 percent less than free stream at a mach number of 0.95.
Thermal conductivity of equimolar water-heavy water mixture considered in reply to comment of Spinner
The head for a high-speed spinner is characterized by a substantially cylindrical body adapted to be mounted at the distal end of a vertically oriented drive shaft. A vacuum chuck with an upwardly facing chamber is circumscribed by an annular surface for receiving in supported relation a silicon chip. An ordered array of low-pressure cavities is defined about the periphery of the body and connected in communication with the chamber via radially extended bores in order to translate low pressures to the chamber as the head is angularly displaced. A pressure differential is thereby established across the chip for securing it to the head.
A graphical method that allows the selection of the attitude orientation of a spinner spacecraft prior to the application of a single fixed magnitude velocity change to effect a desired orbit transfer is presented. The selection of the final pointing vector along or opposite to which the desired Delta-V is applied, takes into account pointing errors due to initial attitude determination uncertainty and execution errors introduced during the rhumb line precession of the spin axis. The characterization of the target space in terms of any achieved orbit parameters of interest as well as the final pointing uncertainty is sufficient to determine the right final attitude that insures satisfaction of mission requirements.
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This paper describes the modeling, analysis, and testing done to determine the DS1 nutation time constant.
Simple, inexpensive apparatus coats semiconductor wafers uniformly with hydrofluoric acid for etching. Apparatus made in part from small commercial electric-fan motor. Features bowl that collects acid. Silicon wafer placed on platform and centered on axis; motor switched on. As wafer spins, drops of hydrofluoric acid applied from syringe. Centrifugal force spreads acid across wafer in fairly uniform sheet.
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