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At least 19 records

Remote Sensing of Solar-Induced Chlorophyll Fluorescence (SIF) in Vegetation: 50 Years of Progress

Remote sensing of solar-induced chlorophyll fluorescence (SIF) is a rapidly advancing front in terrestrial vegetation science, with emerging capability in space-based methodologies and diverse application prospects. Although remote sensing of SIF – especially from space – is seen as a contemporary new specialty for terrestrial plants, it is founded upon a multi-decadal history of research, applications, and sensor developments in active and passive sensing of chlorophyll fluorescence. Current technical capabilities allow SIF to be measured across a range of biological, spatial, and temporal scales. As an optical signal, SIF may be assessed remotely using highly-resolved spectral sensors and state-of-the-art algorithms to distinguish the emission from reflected and/or scattered ambient light. Because the red to far-red SIF emission is detectable non-invasively, it may be sampled repeatedly to acquire spatio-temporally explicit information about photosynthetic light responses and steady-state behaviour in vegetation. Progress in this field is accelerating with innovative sensor developments, retrieval methods, and modelling advances. This review distills the historical and current developments spanning the last several decades. It highlights SIF heritage and complementarity within the broader field of fluorescence science, the maturation of physiological and radiative transfer modelling, SIF signal retrieval strategies, techniques for field and airborne sensing, advances in satellite-based systems, and applications of these capabilities in evaluation of photosynthesis and stress effects. Progress, challenges, and future directions are considered for this unique avenue of remote sensing.

Review

Biogeochemical cycling and remote sensing

Research is underway at the NASA Ames Research Center that is concerned with aspects of the nitrogen cycle in terrestrial ecosystems. An interdisciplinary research group is attempting to correlate nitrogen transformations, processes, and productivity with variables that can be remotely sensed. Recent NASA and other publications concerning biogeochemical cycling at global scales identify attributes of vegetation that could be related or explain the spatial variation in biologically functional variables. These functional variables include net primary productivity, annual nitrogen mineralization, and possibly the emission rate of nitrous oxide from soils.

Peterson, D. L.

Light in Thermal Environments (LITE) Workshop

Light emitted from high temperature black smokers (350 C) at mid-ocean ridge spreading centers has been documented, but the source of this light and its photochemical and biological consequences have yet to be investigated. Preliminary studies indicate that thermal radiation alone might account for the 'glow' and that a novel photoreceptor in shrimp colonizing black smoker chimneys may detect this 'glow.' A more controversial question is whether there may be sufficient photon flux of appropriate wavelengths to support geothermally-driven photosynthesis (GDP) by microorganisms. Although only a very low level of visible and near infrared light may be emitted from any single hydrothermal vent, several aspects of the light make it of more than enigmatic interest. First, the light is clearly linked to geophysical (and perhaps geochemical) processes; its attributes may serve as powerful index parameters for monitoring change in these processes. Second, while the glow at a vent orifice is a very local phenomenon, more expansive subsurface environments may be illuminated, thereby increasing the spatial scale at which biological consequences of this light might be considered. Third, in contrast to intermittent bioluminescent light sources in the deep sea, the light emitted at vents almost certainly glows or flickers continuously over the life of the individual black smokers (years to decades); collectively, light emitted from black smokers along the ocean's spreading centers superimposed on background Cerenkov radiation negates the concept of the deep sea as an environment devoid of abiotic light. Finally, the history of hydrothermal activity predates the origin of life; light in the deep sea has been a continuous phenomenon on a geological time scale and may have served either as a seed or refugium for the evolution of biological photochemical reactions or adaptations.

Source record

Biological cycling of atmospheric trace gases

A detailed critical review was conducted of present knowledge of the influence of biological processes on the cycling of selected atmospheric gas constituents--methane, carbon monoxide, and gaseous compounds of nitrogen (nitrous oxide, ammonia, nitric oxide, and nitrogen dioxide) and sulfur (hydrogen sulfide and sulfur dioxide). The identification was included of biological and other sources of each gas, a survey of abundance measurements reported in the literature, and a review of the atmospheric fate of each contituent. Information is provided on which to base conclusions regarding the importance of biological processes on the atmospheric distribution and surface-atmosphere exchange of each constituent, and a basis for estimating the adequacy of present knowledge of these factors. A preliminary analysis was conducted of the feasibility of monitoring the biologically influenced temporal and spatial variations in abundance of these gases in the atmosphere from satellites.

Hitchcock, D. R.

'Nano' Morphology and Element Signatures of Early Life on Earth: A New Tool for Assessing Biogenicity

The relatively young technology of NanoSIMS is unlocking an exciting new level of information from organic matter in ancient sediments. We are using this technique to characterize Proterozoic organic material that is clearly biogenic as a guide for interpreting controversial organic structures in either terrestrial or extraterrestrial samples. NanoSIMS is secondary ion mass spectrometry for trace element and isotope analysis at sub-micron resolution. In 2005, Robert et al. [1] combined NanoSIMS element maps with optical microscopic imagery in an effort to develop a new method for assessing biogenicity of Precambrian structures. The ability of NanoSIMS to map simultaneously the distribution of organic elements with a 50 nm spatial resolution provides new biologic markers that could help define the timing of life s development on Earth. The current study corroborates the work of Robert et al. and builds on their study by using NanoSIMS to map C, N (as CN), S, Si and O of both excellently preserved microfossils and less well preserved, non-descript organics in Proterozoic chert from the ca. 0.8 Ga Bitter Springs Formation of Australia.

Oehler, D. Z.

Spatial distribution of total, ammonia-oxidizing, and denitrifying bacteria in biological wastewater treatment reactors for bioregenerative life support

Bioregenerative life support systems may be necessary for long-term space missions due to the high cost of lifting supplies and equipment into orbit. In this study, we investigated two biological wastewater treatment reactors designed to recover potable water for a spacefaring crew being tested at Johnson Space Center. The experiment (Lunar-Mars Life Support Test Project-Phase III) consisted of four crew members confined in a test chamber for 91 days. In order to recycle all water during the experiment, an immobilized cell bioreactor (ICB) was employed for organic carbon removal and a trickling filter bioreactor (TFB) was utilized for ammonia removal, followed by physical-chemical treatment. In this study, the spatial distribution of various microorganisms within each bioreactor was analyzed by using biofilm samples taken from four locations in the ICB and three locations in the TFB. Three target genes were used for characterization of bacteria: the 16S rRNA gene for the total bacterial community, the ammonia monooxygenase (amoA) gene for ammonia-oxidizing bacteria, and the nitrous oxide reductase (nosZ) gene for denitrifying bacteria. A combination of terminal restriction fragment length polymorphism (T-RFLP), sequence, and phylogenetic analyses indicated that the microbial community composition in the ICB and the TFB consisted mainly of Proteobacteria, low-G+C gram-positive bacteria, and a Cytophaga-Flexibacter-Bacteroides group. Fifty-seven novel 16S rRNA genes, 8 novel amoA genes, and 12 new nosZ genes were identified in this study. Temporal shifts in the species composition of total bacteria in both the ICB and the TFB and ammonia-oxidizing and denitrifying bacteria in the TFB were also detected when the biofilms were compared with the inocula after 91 days. This result suggests that specific microbial populations were either brought in by the crew or enriched in the reactors during the course of operation.

NASA Discipline Life Support Systems

Remotely sensed indicators of habitat heterogeneity and biological diversity: A preliminary report

The relationship between habitat area, spatial dynamics of the landscape, and species diversity is an important theme in population and conservation biology. Of particular interest is how populations of various species are affected by increasing habitat edges due to fragmentation. Over the last decade, assumptions regarding the effects of habitat edges on biodiversity have fluctuated wildly, from the belief that they have a positive effect to the belief that they have a clearly negative effect. This change in viewpoint has been brought about by an increasing recognition of the importance of geographic scale and a reinterpretation of natural history observations. In this preliminary report from an ongoing project, we explore the use of remote sensing technology and geographic information systems to further our understanding of how species diversity and population density are affected by habitat heterogeneity and landscape composition. A primary feature of this study is the investigation of SAR for making more rigorous investigations of habitat structure by exploiting the interaction between radar backscatter and vegetation structure and biomass. A major emphasis will be on the use of SAR data to define relative structural types based on measures of structural consolidation using the vegetation surface area to volume ratio (SA/V). Past research has shown that SAR may be sensitive to this form of structural expression which may affect biodiversity.

Imhoff, Marc

The Earth Observing System

The Earth Observing System (EOS), the centerpiece of NASA's Mission to Planet Earth, is to study the interactions of the atmosphere, land, oceans, and living organisms, using the perspective of space to observe the earth as a global environmental system. To better understand the role of clouds in global change, EOS will measure incoming and emitted radiation at the top of the atmosphere. Then, to study characteristics of the atmosphere that influence radiation transfer between the top of the atmosphere and the surface, EOS wil observe clouds, water vapor and cloud water, aerosols, temperature and humidity, and directional effects. To elucidate the role of anthropogenic greenhouse gas and terrestrial and marine plants as a source or sink for carbon, EOS will observe the biological productivity of lands and oceans. EOS will also study surface properties that affect biological productivity at high resolution spatially and spectrally.

Wilson, Stan

Production regimes in four eastern boundary current systems

High productivity (maxima ~3 g C m(sup -2)day(sup -1)) of the Eastern Boundary Currents (EBCs), i.e. the California, Peru-Humboldt, Canary and Benguela Currents, is driven by a combination of local forcing and large-scale circulation. The characteristics of the deep water brought to the surface by upwelling favorable winds depend on the large-scale circulation patterns. Here we use a new hydrographic and nutrient climatology together with satellite measurements ofthe wind vector, sea-surface temperature (SST), chlorophyll concentration, and primary production modeled from ocean color to quantify the meridional and seasonal patterns of upwelling dynamics and biological response. The unprecedented combination of data sets allows us to describe objectively the variability for small regions within each current and to characterize the governing factors for biological production. The temporal and spatial environmental variability was due in most regions to large-scale circulation, alone or in combination with offshore transport (local forcing). The observed meridional and seasonal patterns of biomass and primary production were most highlycorrelated to components representing large-scale circulation. The biomass sustained by a given nutrient concentration in the Atlantic EBCs was twice as large as that of the Pacific EBCs. This apparent greater efficiency may be due toavailability of iron, physical retention, or differences in planktonic community structure.

circulation patternsoceans

Searching for Hyperspectral Optical Proxies to Aid Chesapeake Bay Resource Managers in the Detection of Poor Water Quality

Shellfish aquaculture is a growing industry in the Chesapeake Bay. As population grows near the coast, extreme weather events cause a greater volume of pollutant runoff from impervious surfaces and agricultural lands. Resource managers who monitor shellfish beds need reliable information on a variety of water quality indicators at higher frequency than is possible through field monitoring programs and at a higher level of detail than current satellite products can provide. Although many factors causing degraded water quality that can impact human health are not currently discernable by traditional multispectral techniques, hyperspectral imagery offers a new opportunity to detect phytoplankton communities associated with harmful algal blooms and biotoxin production. Together with resource managers in their routine monitoring of sites around the bay from small boats, we have been exploring remotely sensed optical proxies for the detection of harmful algal blooms and sewage. Early warning by remote sensing could guide sampling and improve the efficiency of shellfish bed closures, ultimately improving health outcomes for humans and animals. An extensive network of routine sampling by Chesapeake Bay Program managers makes this is an ideal location to develop and test future satellite data products to support management decisions. Next generation hyperspectral measurements from the future Plankton Aerosol Cloud ocean Ecosystem (PACE) mission at nearly daily frequency, combined with the potential of higher spatial resolution from the Surface Biology and Geology (SBG) observing system recommended in the recent Decadal Survey, along with high frequency observations from the newly selected Geostationary Littoral Imaging and Monitoring Radiometer (GLIMR) Earth Venture Instrument make this a critical time for defining the needs of the aquaculture and resource management community to save lives, time, and money.

Schollaert Uz, Stephanie

Global Intercomparison of hyper-resolution ECOSTRESS coastal sea surface temperature measurements from the Space Station with VIIRS-N20

The ECOSTRESS multi-channel thermal radiometer on the Space Station has an unprecedented spatial resolution of 70 m and a return time of hours to 5 days. It resolves details of oceanographic features not detectable in imagery from MODIS or VIIRS, and has open-ocean coverage, unlike Landsat. We calibrated two years of ECOSTRESS sea surface temperature observations with L2 data from VIIRS-N20 (2019–2020) worldwide but especially focused on important upwelling systems currently undergoing climate change forcing. Unlike operational SST products from VIIRS-N20, the ECOSTRESS surface temperature algorithm does not use a regression approach to determine temperature, but solves a set of simultaneous equations based on first principles for both surface temperature and emissivity. We compared ECOSTRESS ocean temperatures to well-calibrated clear sky satellite measurements from VIIRS-N20. Data comparisons were constrained to those within 90 min of one another using co-located clear sky VIIRS and ECOSTRESS pixels. ECOSTRESS ocean temperatures have a consistent 1.01 ◦C negative bias relative to VIIRS-N20, although deviation in brightness temperatures within the 10.49 and 12.01 µm bands were much smaller. As an alternative, we compared the performance of NOAA, NASA, and U.S. Navy operational split-window SST regression algorithms taking into consideration the statistical limitations imposed by intrinsic SST spatial autocorrelation and applying corrections on brightness temperatures. We conclude that standard bias-correction methods using already validated and well-known algorithms can be applied to ECOSTRESS SST data, yielding highly accurate products of ultra-high spatial resolution for studies of biological and physical oceanography in a time when these are needed to properly evaluate regional and even local impacts of climate change.

Nicolas Weidberg

Do it yourself remote sensing: Generating an inexpensive, high tech, real science lake mapping project for the classroom

The utilization of modest equipment and software revealed bottom contours and water column conditions of a dynamic water body. Classroom discussions of field techniques and equipment capabilities followed by exercises with the data sets in cause-and-effect analysis all contributed to participatory education in the process of science. This project is presented as a case study of the value of engaging secondary and collegiate level students in planning, executing and appraising a real world investigation which they can directly relate to. A 1 km wide bay, experiencing marsh inflow, along an 8 km long lake situated 120 km north of Ottawa, Canada, on the glaciated Canadian Precambrian Shield was mapped in midsummer for submerged topography, bottom composition, temperature profile, turbudity, dissolved oxygen and biota distribution. Low level aerial photographs scanned into image processing software are permitting spatial classification of bottom variations in biology and geology. Instrumentation consisted of a portable sport fishing SONAR depth finder, an electronic lead line multiprobe with photocell, thermistor and dissolved oxygen sensors, a selective depth water sampler, portable pH meter, an underwater camera mounted on a home-made platform with a bottom-contact trigger and a disposable underwater camera for shallow survey work. Sampling transects were referenced using a Brunton hand transit triangulating several shore markers.

Metzger, Stephen M.

Selective functionalization of carbon nanotube tips allowing fabrication of new classes of nanoscale sensing and manipulation tools

Embodiments in accordance with the present invention relate to techniques for the growth and attachment of single wall carbon nanotubes (SWNT), facilitating their use as robust and well-characterized tools for AFM imaging and other applications. In accordance with one embodiment, SWNTs attached to an AFM tip can function as a structural scaffold for nanoscale device fabrication on a scanning probe. Such a probe can trigger, with nanometer precision, specific biochemical reactions or conformational changes in biological systems. The consequences of such triggering can be observed in real time by single-molecule fluorescence, electrical, and/or AFM sensing. Specific embodiments in accordance with the present invention utilize sensing and manipulation of individual molecules with carbon nanotubes, coupled with single-molecule fluorescence imaging, to allow observation of spectroscopic signals in response to mechanically induced molecular changes. Biological macromolecules such as proteins or DNA can be attached to nanotubes to create highly specific single-molecule probes for investigations of intermolecular dynamics, for assembling hybrid biological and nanoscale materials, or for developing molecular electronics. In one example, electrical wiring of single redox enzymes to carbon nanotube scanning probes allows observation and electrochemical control over single enzymatic reactions by monitoring fluorescence from a redox-active cofactor or the formation of fluorescent products. Enzymes ''nanowired'' to the tips of carbon nanotubes in accordance with embodiments of the present invention, may enable extremely sensitive probing of biological stimulus-response with high spatial resolution, including product-induced signal transduction.

Wade, Lawrence A.

Sensor Web in Antarctica: Developing an Intelligent, Autonomous Platform for Locating Biological Flourishes in Cryogenic Environments

The most rigorous tests of the ability to detect extant life will occur where biotic activity is limited by severe environmental conditions. Cryogenic environments are among the most severe-the energy and nutrients needed for biological activity are in short supply while the climate itself is actively destructive to biological mechanisms. In such settings biological activity is often limited to brief flourishes, occurring only when and where conditions are at their most favorable. The closer that typical regional conditions approach conditions that are actively hostile , the more widely distributed biological blooms will be in both time and space. On a spatial dimension of a few meters or a time dimension of a few days, biological activity becomes much more difficult to detect. One way to overcome this difficulty is to establish a Sensor Web that can monitor microclimates over appropriate scales of time and distance, allowing a continuous virtual presence for instant recognition of favorable conditions. A more sophisticated Sensor Web, incorporating metabolic sensors, can effectively meet the challenge to be in "the right place in the right time". This is particularly of value in planetary surface missions, where limited mobility and mission timelines require extremely efficient sample and data acquisition. Sensor Webs can be an effective way to fill the gap between broad scale orbital data collection and fine-scale surface lander science. We are in the process of developing an intelligent, distributed and autonomous Sensor Web that will allow us to monitor microclimate under severe cryogenic conditions, approaching those extant on the surface of Mars. Ultimately this Sensor Web will include the ability to detect and/or establish limits on extant microbiological activity through incorporation of novel metabolic gas sensors. Here we report the results of our first deployment of a Sensor Web prototype in a previously unexplored high altitude East Antarctic Plateau "micro-oasis" at the MacAlpine Hills, Law Glacier, Antarctica.

Delin, K. A.

SIMBIOS Data Product and Algorithm Validation with Emphasis on the Biogeochemical and Inherent Optical Properties

The purpose of our component of the Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) program is to address and quantify the relative accuracy of the various ocean color remote sensing products by means of product and algorithm validation. In order to accomplish these goals, we have been collaborating in an international research program in the Gulf of California designed to examine the spatial and temporal bio-optical variability in this region. In addition, we participated in various cruises of opportunity to supplement our validation data set. Our long-term objectives are to: (1) collect optical and biochemical data in oceanic and coastal regions of interest; (2) provide collected data to the Sea-Viewing Wide Field-of-view Sensor (SeaWiFS) Bio-Optical Archive and Storage System (SeaBASS) database, the SIMBIOS project office, and other interested users; (3) maintain and update instrumentation and data archiving and dissemination systems; and (4) determine spatial and temporal error fields for the biological and geophysical data products from the various ocean color missions.

Zaneveld, J. Ronald V.

Interannual Variation in Phytoplankton Concentration and Community in the Pacific Ocean

Climate events such as El Nino have been shown to have an effect on the biology of our ocean. Because of the lack of data, we still have very little knowledge about the spatial and temporal effect these climate events may have on biological marine systems. In this study, we used the NASA Ocean Biogeochemical Model (NOBM) to assess the interannual variability in phytoplankton community in the Pacific Ocean between 1998 and 2005. In the North Central and Equatorial Pacific Ocean, changes in the Multivariate El Nino Index were associated with changes in phytoplankton composition. The model identified an increase in diatoms of approx.33 % in the equatorial Pacific in 1999 during a La Nina event. This increase in diatoms coincided with a decrease of approx.11 % in cyanobacteria concentration. The inverse relationship between cyanobacteria and diatoms concentration was significant (p<0.05) throughout the period of study. The use of a numerical model allows us to assess the impact climate variability has on key phytoplankton groups known to lead to contrasting food chain at a spatial and temporal resolution unachievable when relying solely on in-situ observations.

Rousseaux, C. S.