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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2017

Zinnia Germination and Lunar Soil Amendment

Germination testing was performed to determine the best method for germinating zinnias. This method will be used to attempt to germinate the zinnia seeds produced in space. It was found that seed shape may be critically important in determining whether a seed will germinate or not. The ability of compost and worm castings to remediate lunar regolith simulant for plant growth was tested. It was found that neither treatment effectively improves plant growth in lunar regolith simulant. A potential method of improving lunar regolith simulant by mixing it with arcillite was discovered.

Reese, Laura↗
NASA NTRS2012

Low Biotoxicity of Mars Analog Soils Suggests that the Surface of Mars May be Habitable for Terrestrial Microorganisms

Recent studies on the interactive effects of hypobaria, low temperatures, and CO2-enriched anoxic atmospheres on the growth of 37 species of mesophilic bacteria identified 14 potential biocidal agents that might affect microbial survival and growth on the martian surface. Biocidal or inhibitory factors include (not in priority): (1) solar UV irradiation, (2) low pressure, (3) extreme desiccating conditions, (4) extreme diurnal temperature fluctuations, (5) solar particle events, (6) galactic cosmic rays, (7) UV-glow discharge from blowing dust, (8) solar UV-induced volatile oxidants [e.g., O2(-), O(-), H2O2, O3], (9) globally distributed oxidizing soils, (10) extremely high salts levels [e.g., MgCl2, NaCl, FeSO4, and MgSO4] in surficial soils at some sites on Mars, (11) high concentrations of heavy metals in martian soils, (12) likely acidic conditions in martian fines, (13) high CO2 concentrations in the global atmosphere, and (14) perchlorate-rich soils. Despite these extreme conditions several studies have demonstrated that dormant spores or vegetative cells of terrestrial microorganisms can survive simulated martian conditions as long as they are protected from UV irradiation. What has not been explored in depth are the effects of potential biotoxic geochemical components of the martian regolith on the survival and growth of microorganisms. The primary objectives of the research included: (1) prepare and characterize Mars analog soils amended with potential biotoxic levels of sulfates, salts, acidifying minerals, etc.; and (2) use the simulants to conduct biotoxicity assays to determine if terrestrial microorganisms from spacecraft can survive direct exposure to the analog soils.

Schuerger, A. C.↗
NASA NTRS2010

Biotoxicity of Mars Analog Soils: Microbial, Dispersal into Desiccated Soils Versus Emplacement in Salt or Ice Inclusions Fluids

Recent evidence from the Opportunity and Spirit rovers and the Mars Express mission suggests that the soils on Mars might be very high in biotoxic materials including sulfate salts, chlorides, and acidifying agents. Yet, very little is known about how the chemistries of Mars soils might affect the survival and growth of terrestrial microorganisms. The primary objectives of the research included: (1) prepare and characterize Mars analog soils amended with potential biotoxic levels of sulfates, chlorides, and acidifying minerals; and (2) use the simulants to conduct a series of toxicology assays to determine if terrestrial microorganisms from spacecraft can survive direct exposure to the biotoxic soils.

Schuerger, A. C.↗
NASA NTRS2010

The Biotoxicity of Mars Soils

Recent evidence from the Opportunity and Spirit rovers suggests that the soils on Mars might be very high in biotoxic materials induding sulfate salts, chlorides, and acidifying agents. Yet, very little is known about how the chemistries of Mars soils might affect the survival and growth of terrestrial microorganisms. The primary objectives of the proposed research will be to: (1) prepare and characterize Mars analog soils amended with potential biotoxic levels of sulfates, chlorides, and acidifying minerals; (2) use the stimulants to conduct a series of toxicology assays to determine if terrestrial microorganisms from spacecraft or extreme environments can survive direct exposure to the biotoxic soils, and (3) mix soils from extreme environments on Earth into Mars analog soils to determine if terrestrial microorganisms can grow and replicate under Martian conditions. The Mars analog soils will be thoroughly characterized by a wide diversity of soil chemistry assays to determine the exact nature of the soluble biotoxic components following hydration. The microbial experiments will be designed to test the effects of Mars stimulants on microbial survival, growth and replication during direct challenge experiments. Toxicology experiments will be designed to mimic terrestrial microbes coming into contact with biotoxic soils with and without liquid water. Results are expected to help" ... characterize the limits of life in ... planetary environments ... " and may help constrain the search for life on Mars.

Kerney, Krystal↗
NASA NTRS2018

Aiding in the Efforts of Bioregenerative Life Support for the Future Residents of Mars and Long-Duration Space Flights Plant Growth in Amended Martian Regolith Simulant

Currently, the goal of plant growth research at KSC (Kennedy Space Center) is to provide a sustainable source of healthy food on long-duration space flights so astronauts and future residents of Mars can get the nutrition they need and produce food, recycle the atmosphere, and aid in recycling water. The sustainable production of food will aid in the efforts of closed life support. Plants have a vital application for bioregenerative life support as demands for food and oxygen can be provided through photosynthesis, while the carbon dioxide from human respiration is removed. In order for a growing medium to provide for plant growth, it needs to provide support, oxygen, ions, and liquid to the plant. This summer research determines how amended Martian regolith simulant acts as a growing medium for plants through looking at the structural properties of each amendment. Mars is covered with regolith which is crushed volcanic rock that is composed of a high amount of perchlorate salts. The composition of Martian soil is such that it is roughly basaltic and consists of sulfur, nitrates, and oxychlorine species. Martian regolith simulants have been generated to further analyze plant growth and aim to replicate features of the reference sample. The JSC (Johnson Space Center) Mars-1A regolith simulant resembles Martian regolith at the Viking I landing site. The objectives of the research this summer at Kennedy Space Center are to devise a plant growth in amended JSC Mars-1A Martian regolith simulant and perform crop studies to assess the performance of full duration crop growth in amended Martian regolith simulant. This research will provide a deeper understanding of how the combination of soil amendments to Martian regolith has a synergistic effect on improving crop production The plant growth experiment will be completed with three treatments each containing inorganic and organic soil amendments.

Onsay, Daphne↗
NASA NTRS2001

Effects of Potassium Permanganate Oxidation on Subsurface Microbial Activity

In situ chemical oxidation has the potential for degrading large quantities of organic contaminants and can be more effective and timely than traditional ex situ treatment methods. However, there is a need to better characterize the potential effects of this treatment on natural processes. This study focuses on potential inhibition to anaerobic dechlorination of trichloroethene (TCE) in soils from a large manufacturing facility as a result of in situ oxidation using potassium permanganate (KMn04)Previous microcosm studies established that natural attenuation occurs on-site and that it is enhanced by the addition of ethanol to the system. A potential remediation scheme for the site involves the use of potassium permanganate to reduce levels of TCE in heavily contaminated areas, then to inject ethanol into the system to "neutralize" excess oxidant and enhance microbial degradation. However, it is currently unknown whether the exposure of indigenous microbial populations to potassium permanganate may adversely affect biological reductive dechlorination by these microorganisms. Consequently, additional microcosm studies were conducted to evaluate this remediation scheme and assess the effect of potassium permanganate addition on biological reductive dechlorination of TCE. Samples of subsurface soil and groundwater were collected from a TCE-impacted area of the site. A portion of the soil was pretreated with nutrients and ethanol to stimulate microbial activity, while the remainder of the soil was left unamended. Soil/groundwater microcosms were prepared in sealed vials using the nutrient-amended and unamended soils, and the effects of potassium permanganate addition were evaluated using two permanganate concentrations (0.8 and 2.4 percent) and two contact times (1 and 3 weeks). TCE was then re-added to each microcosm and TCE and dichloroethene (DCE) concentrations were monitored to determine the degree to which microbial dechlorination occurred following chemical oxidation. Evidence of microbial degradation was generally detected within four weeks after TCE addition. Increases in DCE concentrations were consistent with decreases in TCE. The concentration of TCE in the nutrient-amended samples exposed to 2.4% KMnO4 for one week degraded somewhat more slowly than the samples exposed to the 0.8% KMnO4. The rates of degradation did not correlate with the length of KMn04 exposure for the nutrient-amended microcosms. Microbial degradation of TCE in the unamended microcosms was generally similar to that observed in the nutrient-amended microcosms. One treatment condition (unamended, one week exposure, 2.4% KMnO4) was exposed to elevated levels of ethanol and showed little evidence of degradation. It is suspected that the high levels of ethanol were toxic to the microorganisms. The results of the study indicate that exposure of indigenous soil and groundwater microbial populations to KMnO4 at concentrations of 0.8 to 2.4% do not impair the ability of the microbial populations to dechlorinate TCE. Consequently, the combination of chemical oxidation followed by enhanced biological reductive dechlorination appears to be a viable remedial strategy for highly-impacted subsurface areas of the site.

Rowland, Martin A.↗
NASA NTRS1974

Arthrobacter globiformis and its bacteriophage in soil

An attempt was made to correlate bacteriophages for Arthrobacter globiformis with soils containing that bacterium. The phages were not detected unless the soil was nutritionally amended (with glucose or sucrose) and incubated for several days. Phage was continuously produced after amendment without the addition of host Arthrobacter. These results indicate that the bacteriophage is present in a masked state and that the bacteria are present in an insensitive form which becomes sensitive after addition of nutrient.

Casida, L. E., Jr.↗
NASA NTRS1999

Population of Nitrifying Bacteria and Nitrification in Ammonium Saturated Clinoptilolite

As humans begin to spend longer periods of time in space, plants will be incorporated into life support systems. Ammonium saturated clinoptilolite is one plant growth substrate but a balance between ammonium and nitrate is needed. A laboratory study was conducted to determine effects of nitrifying bacteria on ammonium concentrations and kinetics of nitrification. Columns containing clinoptilolite substrate amended with nitrifying bacteria obtained from soil enrichment were analyzed weekly for a 90 day period. The enrichment culture initially contained 1 x 10(exp 5) ammonium oxidizing bacteria and 1 x 10(exp 2) nitrite oxidizing bacteria per gram of substrate. Populations of ammonium oxidizing bacteria increased to 1 x 10(exp 6) and nitrite oxidizing bacteria increased to 1 x 10(exp 3) per gram of substrate. The nitrification rate was approximately 0.25mg NO3(-)-N/kg.hr. Experiments were also conducted to enumerate nitrifying bacteria in a clinoptilolite substrate used to grow wheat (Triticum aestivum L.). Seventy days following the initial inoculation with an unknown number of commercial nitrifying bacteria, 1 x 10(exp 5) ammonium oxidizing bacteria per gram of substrate were present. The number of nitrite oxidizing bacteria was between 1 x 10(exp 3) to 10(exp 4) per gram of substrate as measured by the most probable number method. Nitrification rates were approximately 0.20mg NO3(-)-N/kg.hr. Clinoptilolite readily exchanged sufficient concentrations of ammonium to support nitrifying bacteria and they survived well in this medium.

McGilloway, R. L.↗
NASA NTRS1979

Minute tubular forms in soil

Large numbers of long, straight, flattened structures were observed during an electron-microscope study of bacteriophage in aqueous extracts. These structures were called tubules and ranged in width from 10 to 50 nm. Materials and methods were discussed relative to extraction of tubules from soil, electron microscopy, quantitation of tubules in filtrate suspension, tests performed on tubules, plaque formation, and nutrient amendments. It is found that all of the tubules recovered from soil are broken at one or both ends. They are present in surface soils but not in a subsurface sample. Their numbers decrease during bacterial multiplication in soil or broth-containing soil. The tubules appear to be composed of protein that could be disintegrated to liberate nonprotein fibers. A possible clue to the nature of the tubules is their apparent relation to soil bacteria.

Casida, L. E., Jr.↗
NASA NTRS1992

NO versus N2O emissions from an NH4(+)-amended Bermuda grass pasture

An enclosure technique is used to monitor soil NO and N2O emissions during early summer regrowth of Bermuda grass (Cynodon dactylon) on sandy loam in a humid, subtropical region of southern Texas. The evolution of both gases was substantially higher from plots harvested at the beginning of the experiment and fertilized five days later with 52 kg N/ha as (NH4)2SO4 than from plots not harvested or fertilized. Emission of NO, but not N2O, was stimulated by clipping and removing the grass, probably because eliminating the shading provided by the dense grass canopy changed these plots from cooler to warmer than unharvested plots, thereby stimulating the activity of soil microorganisms responsible for NO production. Neither gas flux was significantly affected by application of N until the next rainfall dissolved and moved the surface-applied fertilizer into the soil. Immediately thereafter, emissions of NO and N2O increased dramatically to peaks of 160 and 12 g N/ha/d, respectively, and then declined at rates that closely parallel the nitrification rate of added NH4(+), indicating that the gases resulted from the activity of nitrifying microorganisms, rather than denitrifiers. Nitric oxide emissions during the nine-week measurement period averaged 7.2 times greater than N2O emissions and accounted for 3.2 percent of the added N. The data indicate that humid, subtropical grasslands, which not only have large geographical extent but also have been subject to intense anthropogenic disturbance, contribute significantly to the global atmospheric NO(x) budget.

Hutchinson, G. L.↗
NASA NTRS1972

Detection of soil microorganism in situ by combined gas chromatography mass spectrometry

Experimental tests were made to determine whether analysis of volatile metabolic products, formed in situ, is a viable procedure for an extraterrestrial life detection system. Laboratory experiments, carried out under anaerobic conditions with addition of carbon source, extended to include a variety of soils and additional substrates. In situ experiments were conducted without amendment using a vacuum sampling system.

Alexander, M.↗
NASA NTRS1998

Slow-Release Fertilizer

Under an SBIR (Small Business Innovative Research), ZeoponiX, Inc., introduced ZeoPro. This product is used as a fertilizer/soil amendment for golf courses, ball fields, greenhouse and horticultural uses. A combination of superior growth medium and soil conditioner allow for nutrient supplementation and high efficiency delivery of nutrients throughout the plant. ZeoPro provides a balanced nutrient system for major, minor, and trace nutrients.

Source record↗
NASA NTRS2000

Zeoponic Plant Growth Substrate Development at the Johnson Space Center and Possible Use at a Martian Outpost

The National Aeronautics and Space Administration (NASA) Johnson Space Center (JSC) is developing a substrate, termed zeoponics, that will slowly release all of the essential nutrients into solution for plant growth experiments in advanced life support system testbeds. This substrate is also potentially useful in the near future on the Space Shuttle and International Space Station and could eventually be used at an outpost on Mars. Chemical analyses of the Martian soil by the Viking and Mars Pathfinder missions have indicated that several of the elements required for plant growth are available in the soil. It may be possible to use the martian soil as the bulk substrate for growing food crops, while using smaller amounts of zeoponic substrate as an amendment to rectify any nutrient deficiencies.

Gruener, John E.↗
NASA NTRS2023

In Situ Chemical Reduction Interim Measures Work Plan Hot Spot 3 Mobile Launcher Platform Rehabilitation Sites / Vehicle Assembly Building Area (SWMU 056) Kennedy Space Center, Florida

This document presents the design details of an In Situ Chemical Reduction (ISCR) Interim Measures Work Plan for Hot Spot 3 within Mobile Launcher Platform Rehabilitation Sites/Vehicle Assembly Building Area, Solid Waste Management Unit (SWMU) 056 (SWMU 056), located at the John F. Kennedy Space Center, Florida. The interim measures (IM) treatment zone encompasses an area identified as the High Concentration Plume (HCP), which is defined as the area with chlorinated volatile organic compound (CVOC) groundwater concentrations greater than their respective Natural Attenuation Default Concentration (NADC). The objective of the IM is to remove CVOC mass within the treatment area to levels that facilitate a transition to natural attenuation monitoring. The IM design consists of the selected ISCR amendment, Provect-IR, which is a mixture of solid reagents in a single product that promotes enhanced reductive dechlorination and ISCR. ProvectIR includes natural antimethanogenic compounds; multiple hydrophilic, nutrient-rich organic carbon sources; zero valent iron; chemical oxygen scavengers; and vitamin and mineral sources. The proposed treatment area for this IM encompasses less than 1 acre and includes three vertical treatment intervals to reach the NADC plumes that vary per CVOC and depth: 4 to 10 feet below land surface (bls), 10 to 18 feet bls, and 42 to 52 feet bls. The amendment will be distributed through 16 injection points (IP01 through IP16) using dosing rates ranging from 0.4% to 0.6% of soil mass within the treatment interval and a 15% or 20% slurry strength. Dosage rates and slurry strength are based on groundwater geochemistry, soil characteristics, and contaminant concentrations. To aid in the evaluation of the ISCR IM performance, six performance monitoring wells will be installed following injections and sampled semiannually. CVOC parameters will be sampled until concentrations reduce to below their NADC. Underground Injection Control parameters will be sampled until concentrations decrease to background levels or to levels that meet respective groundwater standards. Sampling frequency will be evaluated annually and modified as needed. The path forward for the ISCR IM implementation includes preparing the IM Implementation Work Plan, ISCR injections, monitoring well installation, performance monitoring, and reporting.

Randall K. Sillan↗
NASA NTRS1980

Soil erosion and sediment control laws. A review of state laws and their natural resource data requirements

Twenty states, the District of Columbia, and the Virgin Islands enacted erosion and sediment control legislation during the past decade to provide for the implementation or the strengthening of statewide erosion and sediment control plans for rural and/or urban lands. That legislation and the state programs developed to implement these laws are quoted and reviewed. The natural resource data requirements of each program are also extracted. The legislation includes amendments to conservation district laws, water quality laws, and erosion and sediment control laws. Laws which provides for legislative review of administrative regulations and LANDSAT applications and/or information systems that were involved in implementing or gathering data for a specific soil erosion and sediment control program are summarized as well as principal concerns affecting erosion and sediment control laws.

Klein, S. B.↗
NASA NTRS2015

Kennedy Space Center Press Site (SWMU 074) Interim Measure Report

This report summarizes the Interim Measure (IM) activities conducted at the Kennedy Space Center (KSC) Press Site ("the Press Site"). This facility has been designated as Solid Waste Management Unit 074 under KSC's Resource Conservation and Recovery Act Corrective Action program. The activities were completed as part of the Vehicle Assembly Building (VAB) Area Land Use Controls Implementation Plan (LUCIP) Elimination Project. The purpose of the VAB Area LUCIP Elimination Project was to delineate and remove soil affected with constituents of concern (COCs) that historically resulted in Land Use Controls (LUCs). The goal of the project was to eliminate the LUCs on soil. LUCs for groundwater were not addressed as part of the project and are not discussed in this report. This report is intended to meet the Florida Department of Environmental Protection (FDEP) Corrective Action Management Plan requirement as part of the KSC Hazardous and Solid Waste Amendments permit and the U.S. Environmental Protection Agency's (USEPA's) Toxic Substance Control Act (TSCA) self-implementing polychlorinated biphenyl (PCB) cleanup requirements of 40 Code of Federal Regulations (CFR) 761.61(a).

Measures↗
NASA NTRS2010

Reducing Tick-Borne Disease in Alabama: Linking Health Risk Perception with Spatial Analysis Using the NASA Earth Observing System

Lyme disease (LD) accounts for most vector-borne disease reports in the U.S., and although its existence in Alabama remains controversial, other tick-borne illnesses (TBI) such as Southern Tick-Associated Rash Illness (STARI) pose a health concern in the state. Phase One of the Marshall Space Flight Center-UAB DEVELOP study of TBI identified the presence of the chain of infection for LD (Ixodes scapularis ticks carrying Borrelia burgdorferi bacteria) and STARI (Amblyomma americanum ticks and an as-yet-unconfirmed agent) in Alabama. Both LD and STARI are associated with the development of erythema migrans rashes around an infected tick bite, and while treatable with oral antibiotics, a review of educational resources available to state residents revealed low levels of prevention information. To improve prevention, recognition, and treatment of TBI in Alabama, Phase Two builds a health communication campaign based on vector habitat mapping and risk perception assessment. NASA Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) satellite imagery identified likely tick habitats using remotely sensed measurements of vegetation vigor (Normalized Difference Vegetation Index) and soil moisture. Likely tick habitats, identified as those containing both high vegetation density and soil moisture, included Oak Mountain State Park, Bankhead National Forest, and Talladega National Forest. To target a high-risk group -- outdoor recreation program participants at Alabama universities -- the study developed a behavior survey instrument based on existing studies of LD risk factors and theoretical constructs from the Social Ecological Model and Health Belief Model. The survey instrument was amended to include geographic variables in the assessment of TBI knowledge, attitudes, and prevention behaviors, and the vector habitat model will be expanded to incorporate additional environmental variables and in situ data. Remotely sensed environmental data combined with risk perception assessments inform an ongoing outreach campaign consisting of stakeholder meetings and educational seminars.

Hemmings, S.↗
NASA NTRS2016

Interim Measures Report for the Headquarters Building Area Location of Concern (LOC) 2E East SWMU 104 John F. Kennedy Space Center, Florida

The Hazardous and Solid Waste Amendment portion of the National Aeronautics and Space Administration (NASA) Resource Conservation and Recovery Act (RCRA) Permit issued by the Florida Department of Environmental Protection (FDEP), requires identification and evaluation of all known Solid Waste Management Units (SWMUs) and Locations of Concern (LOCs) located on Kennedy Space Center (KSC) property. The KSC Headquarters Building Area (KHQA) has been identified as SWMU 104 under KSC's RCRA Program. This report summarizes the Interim Measure (IM) conducted by Geosyntec Consultants (Geosyntec) for NASA under Indefinite Delivery Indefinite Quantity Contract NNK12CA13B at the KHQA to mitigate potential exposure to polychlorinated biphenyl (PCB)-affected media at the eastern side of LOC 2E. The IM activities were conducted in June and July 2015 to remediate PCBs above the FDEP Residential Direct-Exposure (R-) Soil Cleanup Target Level (SCTL) of 0.5 milligram per kilogram (mg/kg) established by Chapter 62-777, Florida Administrative Code. The IM was performed in accordance with the IM Work Plan (IMWP) approved by the FDEP, dated August 2012. IM activities were conducted in accordance with the KSC Generic PCB Work Plan (NASA 2007).

Sager, Eric D.↗