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We are developing a nanofluidic size exclusion chromatograph system that can be incorporated into a miniature lab-on-a-chip devices which will provide detailed information on the geochemical and biological history of soil, ice, and water samples by identifying and qualifying a broad range of macromolecular polar and nonpolar organic compounds.
The areas of the United States that were not available as potential sites for receiving antennas that are an integral part of the Satellite Power System concept are presented. Thirty-six variables with the potential to exclude the rectenna were mapped and coded in a computer. Some of these variables exclude a rectenna from locating within the area of its spatial influence, and other variables potentially exclude the rectenna. These maps of variables were assembled from existing data and were mapped on a grid system.
We present new high-resolution topographic models of 4 high-priority lunar south pole landing sites based exclusively on the laser altimetry data acquired by the Lunar Orbiter Laser Altimeter (LOLA) onboard the Lunar Reconnaissance Orbiter. By iteratively adjusting the LOLA tracks to the LOLA-based digital elevation model (LDEM) in a self-consistent fashion, we reduce the orbital geolocation errors by over a factor of 10 such that the new ground track geolocation uncertainty is ~10–20 cm horizontally and ~2–4 cm vertically over each 16 × 16 km region. These new and improved 5 m/pix LDEMs will be useful to constrain higher-resolution topographic models derived from imagery, which are not as well controlled geodetically and which can be hindered by shadows. We developed a method to estimate surface height uncertainty in the new LDEMs, which accounts for the reduced orbital errors and interpolation errors by assuming a fractal behavior for the short-scale topography. The LDEM surface height and slope uncertainties have typical RMS values of ~0.30–0.50 m and ~1.5–2.5°, respectively. Finally, we examine how height uncertainties propagate to variations in horizon elevation and thus the predicted illumination conditions at these polar latitudes, and we show how this error characterization can inform landing site studies.
Specific areas were analyzed and identified as high priority for more in-depth analysis. These areas were: (1) rectenna constructability; (2) satellite constructability; (3) support systems constructability; (4) space environmental analysis, and (5) special end-to-end analyses. Baseline requirements specified coplanar solar blankets and an end mounted antenna, utilizing either GaAlAs solar cells and employing a CR of 2, or Si cells. Several configurations were analyzed. Utilizing the preferred configuration as a baseline, a satellite construction base was defined, precursor operations incident to establishment of orbital support facilities identified, and the satellite construction sequence and procedures developed. Since the baseline specifies sixty instead of one hundred and twenty satellites to be constructed in a thirty year period, mass flow to orbit requirements were revised and new traffic models established. Launch site requirements (exclusive of actual launch operations) in terms of manpower and building space were defined.
Dynamical orbital techniques were employed to estimate the center-of-mass station coordinates of six C-band radars located in the designated primary GEOS-C radar altimeter calibration area. This work was performed in support of the planned GEOS-C mission (December, 1974 launch). The sites included Bermuda, Grand Turk, Antigua, Wallops Island (Virginia), and Merritt Island (Florida). Two sites were estimated independently at Wallops Island yielding better than 40 cm relative height recovery, with better than 10 cm and 1 m (relative) recovery for phi and gamma respectively. Error analysis and comparisons with other investigators indicate that better than 2 m relative recovery was achieved at all sites. The data used were exclusively that from the estimated sites and included 18 orbital arcs which were less than two orbital revolutions in length, having successive tracks over the area. The techniques employed here, given their independence of global tracking support, can be effectively employed to improve various geodetic datums by providing very long and accurate baselines. The C-band data taken on GEOS-C should be employed to improve such geodetic datums as the European-1950 using similar techniques.
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The distribution of Hg is considered both in terms of its availability in soil fractions and the relationship between Hg in plant samples and Hg in ambient soils or other supportive media. The plants were grouped by habitat into epipedic-epiphytic (mosses, lichens) and endopedic-aquatic-marine (Basidiomycetes and algae) samples; nonvascular and vascular forms were also distinguished. Sources included Alaska, Hawaii, New England and Iceland. Brief consideration was also given to Hg distribution in a plant-animal-soil community. Data were expressed in terms of plant Hg content and plant substratum concentration ratio. Average Hg contents and concentration ratios, and modal ranges for the ratios were determined. The results showed similar average Hg contents in all groups (126 to 199 ppb) but a low value (84 ppb) in the lichens; terrestrial forms had ratios of 3.5 to 7.6 whereas the marine algae yielded a figure of 78.7. A secondary mode in the range 0 to 0.1 appeared only in the Alaska-New England Group, over 500 km distant from active thermal sites. Evidence for both exclusion and concentration behavior was obtained.
Future missions envisioned for both human and robotic exploration demand increasing communication capacity through the use of Ka-band communications. The Ka-band channel, being more sensitive to weather impairments, presents a unique trade-offs between data storage, latency, data volume and reliability. While there are many possible techniques for optimizing Ka-band operations such as adaptive modulation and coding and site-diversity, this study focus exclusively on the use of adaptive data rate (ADR) to achieve significant improvement in the data volume-availability tradeoff over a wide range of link distances for near Earth and Mars exploration. Four years of Kepler Ka-band downlink symbol signal-to-noise (SNR) data reported by the Deep Space Network were utilized to characterize the Ka-band channel statistics at each site and conduct various what-if performance analysis for different link distances. We model a notional closed-loop adaptive data rate system in which an algorithm predicts the channel condition two-way light time (TWLT) into the future using symbol SNR reported in near-real time by the ground receiver and determines the best data rate to use. Fixed and adaptive margins were used to mitigate errors in channel prediction. The performance of this closed-loop adaptive data rate approach is quantified in terms of data volume and availability and compared to the actual mission configuration and a hypothetical, optimized single rate configuration assuming full a priori channel knowledge.
The miniaturized Moessbauer (MB) spectrometers MIMOS II [1] on board of the two Mars Exploration Rovers Spirit and Opportunity have obtained more than 600 spectra of more than 300 different rock and soil targets [2-7]. Both instruments have simultaneously collected 6.4 keV X-ray and 14.4 keV .-ray spectra in backscattering geometry [1]. With Spirit's MB spectrometer, 6.4 keV and 14.4 keV spectra have been obtained for all targets through sol 461. After this date, only 14.4 keV spectra were collected. With Opportunity's spectrometer, 6.4 keV and 14.4 keV spectra have been collected for all targets to date. The Fe-mineralogy of rock and soil targets at both landing sites reported to date has been exclusively extracted from 14.4 keV spectra [2-5]. The comparison of 6.4 keV and 14.4 keV spectra provides depth selective information about a sample, but interpretation is not always straightforward [8].
As the transportation of ISS payloads and cargo shifts from KSC to other launch sites, close coordination of ground safety review processes would be of benefit to all parties. The benefit would have the launch sites receiving consistent data that would require less effort to review while still meeting their needs. Until recently, ground safety focus for the ISS program has been almost exclusively for prelaunch processing at KSC/post-landing processing at KSC/DFRC Each launch site, used by the ISS Program, has a ground safety review process. Ground safety viewed as local prerogative. Up till now, ground processing has consisted of low risk/low hazard items; but this will not always be the case. Recent coordination issues associated with the ground safety review of ORU's to be processed at Tanegashima for HTV-2, illustrate that IP ground safety review processes are not well understood by the ISS community at large. Confusion for data providers (US only?). Lack of internal review process for data being submitted to launch sites can lead to inconsistent submittals. NCRs/HRs. Majority of IP ground safety requirements are based upon old KHB 1700.7 (now KNPR 8715.3, Chapter 20). Proposals include: Establish a ground safety working group as part of the MS&MAP. Search for efficiencies in requirements and data submittal processes. Document processes in NSTS 13830/SSP 30599. Each launch site report out its payload ground safety status at the F2F (Monthly's as required). Completions/due dates/NCRs/issues/changes. Establish internal processes for review of ground safety submittals.
Current research and evaluation of the physical resources requirements for the Satellite Power System (SPS) concentrates on three topics: land requirements and the siting of rectennas; the environmental impacts of the rectenna siting; and the materials requirements. The first two focus exclusively on the Earth based element of the SPS while the materials assessment considered requirements for both the space and Earth systems.
Extracellular responses from single horizontal semicircular canal afferents in two rhesus monkeys were studied after recovery from a 14-day biosatellite (Cosmos 2044) orbital spaceflight. On the 1st postflight day, the mean gain for 9 different horizontal canal afferents, tested using one or several different passive yaw rotation waveforms, was nearly twice that for 20 horizontal canal afferents similarly tested during preflight and postflight control studies. Adaptation of the afferent response to passive yaw rotation on the 1st postflight day was also greater. These results suggest that at least one component of the vestibular end organ (the semicircular canals) is transiently modified after exposure to 14 days of microgravity. It is unclear whether the changes are secondary to other effects of microgravity, such as calcium loss, or an adaptive response. If the response is adaptive, then this report is the first evidence that the response of the vestibular end organ may be modified (presumably by the central nervous system via efferent connections) after prolonged unusual vestibular stimulation. If this is the case, the sites of plasticity of vestibular responses may not be exclusively within central nervous system vestibular structures, as previously believed.
Under NASA's Project Prometheus, the Nuclear Systems Program, the Jet Propulsion Laboratory, Pratt & Whitney Rocketdyne, and Teledyne Energy Systems have teamed with a number of universities, under the Segmented Thermoelectric Multicouple Converter (STMC) program, to develop the next generation of advanced thermoelectric converters for space reactor power systems. Work on the STMC converter assembly has progressed to the point where the lower temperature stage of the segmented multicouple converter assembly is ready for laboratory testing and the upper stage materials have been identified and their properties are being characterized. One aspect of the program involves mission application studies to help define the potential benefits from the use of these STMC technologies for designated NASA missions such as the lunar base power station where kilowatts of power are required to maintain a permanent manned presence on the surface of the moon. A modular 50 kWe thermoelectric power station concept was developed to address a specific set of requirements developed for this mission. Previous lunar lander concepts had proposed the use of lunar regolith as in-situ radiation shielding material for a reactor power station with a one kilometer exclusion zone radius to minimize astronaut radiation dose rate levels. In the present concept, we will examine the benefits and requirements for a hermetically-sealed reactor thermoelectric power station module suspended within a man-made lunar surface cavity. The concept appears to maximize the shielding capabilities of the lunar regolith while minimizing its handling requirements. Both thermal and nuclear radiation levels from operation of the station, at its 100-m exclusion zone radius, were evaluated and found to be acceptable. Site preparation activities are reviewed and well as transport issues for this concept. The goal of the study was to review the entire life cycle of the unit to assess its technical problems and technology needs in all areas to support the development, deployment, operation and disposal of the unit.
NASA’s first asteroid sample return mission, OSIRIS-REx, collected a sample from the surface of near-Earth asteroid Bennu in October 2020 and will deliver it to Earth in September 2023. Selecting a sample collection site on Bennu’s surface was challenging due to the surprising lack of large ponded deposits of regolith particles exclusively fine enough (≤ 2 cm diameter) to be ingested by the spacecraft’s Touch-and-Go Sample Acquisition Mechanism (TAGSAM). Here we describe the Sampleability Map of Bennu, which was constructed to aid in the selection of candidate sampling sites and to estimate the probability of collecting sufficient sample. “Sampleability” is a numeric score that expresses the compatibility of a given area’s surface properties with the sampling mechanism. The algorithm that determines sampleability is a best fit functional form to an extensive suite of laboratory testing outcomes tracking the TAGSAM performance as a function of four observable properties of the target asteroid. The algorithm and testing were designed to measure and subsequently predict TAGSAM collection amounts as a function of the minimum particle size, maximum particle size, particle size frequency distribution, and the tilt of the TAGSAM head off the surface. The sampleability algorithm operated at two general scales, consistent with the resolution and coverage of data collected during the mission. The first scale was global and evaluated nearly the full surface. Due to Bennu’s unexpected boulder coverage and lack of ponded regolith deposits, the global sampleability efforts relied heavily on additional strategies to find and characterize regions of interest based on quantifying and avoiding areas heavily covered by material too large to be collected. The second scale was site-specific and used higher-resolution data to predict collected mass at a given contact location. The rigorous sampleability assessments gave the mission confidence to select the best possible sample collection site and directly enabled successful collection of hundreds of grams of material.
Deuterium excess (d) is interpreted in conventional paleoclimate reconstructions as a tracer of oceanic source region conditions, such as temperature, where precipitation originates. Previous studies have adopted co-isotopic approaches to estimate past changes in both site and oceanic source temperatures for ice core sites using empirical relationships derived from conceptual distillation models, particularly Mixed Cloud Isotopic Models (MCIMs). However, the relationship between d and oceanic surface conditions remains unclear in past contexts. We investigate this climate-isotope relationship for sites in Greenland and Antarctica using multiple simulations of the water isotope-enabled Goddard Institute for Space Studies (GISS) ModelE-R general circulation model and apply a novel suite of model vapor source distribution (VSD) tracers to assess d as a proxy for source temperature variability under a range of climatic conditions. Simulated average source temperatures determined by the VSDs are compared to synthetic source temperature estimates calculated using MCIM equations linking d to source region conditions. We show that although deuterium excess is generally a faithful tracer of source temperatures as estimated by the MCIM approach, large discrepancies in the isotope-climate relationship occur around Greenland during the Last Glacial Maximum simulation, when precipitation seasonality and moisture source regions were notably different from present. This identified sensitivity in d as a source temperature proxy suggests that quantitative climate reconstructions from deuterium excess should be treated with caution for some sites when boundary conditions are significantly different from the present day. Also, the exclusion of the influence of humidity and other evaporative source changes in MCIM regressions may be a limitation of quantifying source temperature fluctuations from deuterium excess in some instances.
Selective metal coordination is central to the functions of metalloproteins:1,2 each metalloprotein must pair with its cognate metallocofactor to fulfl its biological role3 . However, achieving metal selectivity solely through a three-dimensional protein structure is a great challenge, because there is a limited set of metal-coordinating amino acid functionalities and proteins are inherently fexible, which impedes steric selection of metals3,4 . Metal-binding afnities of natural proteins are primarily dictated by the electronic properties of metal ions and follow the Irving–Williams series5 (Mn2+ < Fe2+ < Co2+ < Ni2+ Zn2+) with few exceptions6,7 . Accordingly, metalloproteins overwhelmingly bind Cu2+ and Zn2+ in isolation, regardless of the nature of their active sites and their cognate metal ions1,3,8 . This led organisms to evolve complex homeostatic machinery and non-equilibrium strategies to achieve correct metal speciation1,3,8–10. Here we report an artifcial dimeric protein, (AB)2, that thermodynamically overcomes the Irving–Williams restrictions in vitro and in cells, favouring the binding of lower-Irving–Williams transition metals over Cu2+, the most dominant ion in the Irving–Williams series. Counter to the convention in molecular design of achieving specifcity through structural preorganization, (AB)2 was deliberately designed to be fexible. This fexibility enabled (AB)2 to adopt mutually exclusive, metal-dependent conformational states, which led to the discovery of structurally coupled coordination sites that disfavour Cu2+ ions by enforcing an unfavourable coordination geometry. Aside from highlighting fexibility as a valuable element in protein design, our results illustrate design principles for constructing selective metal sequestration agents.
Calcium-dependent protein kinases (CDPKs) are specific to plants and some protists. Their activation by calcium makes them important switches for the transduction of intracellular calcium signals. Here, we identify the subcellular targeting potentials for nine CDPK isoforms from Arabidopsis, as determined by expression of green fluorescent protein (GFP) fusions in transgenic plants. Subcellular locations were determined by fluorescence microscopy in cells near the root tip. Isoforms AtCPK3-GFP and AtCPK4-GFP showed a nuclear and cytosolic distribution similar to that of free GFP. Membrane fractionation experiments confirmed that these isoforms were primarily soluble. A membrane association was observed for AtCPKs 1, 7, 8, 9, 16, 21, and 28, based on imaging and membrane fractionation experiments. This correlates with the presence of potential N-terminal acylation sites, consistent with acylation as an important factor in membrane association. All but one of the membrane-associated isoforms targeted exclusively to the plasma membrane. The exception was AtCPK1-GFP, which targeted to peroxisomes, as determined by covisualization with a peroxisome marker. Peroxisome targeting of AtCPK1-GFP was disrupted by a deletion of two potential N-terminal acylation sites. The observation of a peroxisome-located CDPK suggests a mechanism for calcium regulation of peroxisomal functions involved in oxidative stress and lipid metabolism.
The Lunar Science for Landed Missions workshop was held on 10–12 January, 2018 at NASA Ames Research Center and was attended by lunar scientists, representatives from commercial companies, and representative of various international space agencies including the Japan Aerospace Exploration Agency and the European Space Agency. The workshop was cosponsored by the Solar System Exploration Research Virtual Institute (SSERVI) and the Lunar Exploration Analysis Group (LEAG). The primary goal of the workshop was to produce a set of high‐priority landing site targets, generated by the lunar science community, for near‐term lunar missions. The scope of such missions was aimed primarily, but not exclusively, at commercial exploration companies with interests in pursuing ventures on the surface of the Moon. The results of this workshop were published in a report that summarized the findings of the workshop and provided an analysis of priority landing sites and how missions to these sites would meet key science and exploration goals determined by NASA and the scientific community (Jawin, et al, 2019). This presentation for JpGU will review the landing sites advocated by the workshop, highlight characteristics that make them high-priority sites, and showcase the sites using visualizations generated using NASA’s Moon Trek data visualization and analysis portal (Day and Law, 2017).