The vacuum probe for removing organisms for counting
Vacuum probe for assay and recovery of remaining microorganisms from large heat cycle sterilized surfaces
Morris, M. E.↗
SEARCH · Engineering Papers
Results for “probe counts”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Spatial Transferability of Machine Learning Based Volume Estimation Models
High-quality traffic volume data is essential for efficient transportation planning and operations. However, such high-quality data is expensive to collect, owing primarily to the high capital cost of installing and maintaining continuous counting stations (CCSs). Recent availability of probe-based vehicle data offers a cost-effective solution for increasing the observability of traffic volumes. However, having ample ground truth traffic data is a prerequisite for developing robust volume estimation models. Though this might not be a big issue in many states, states with scarce CCS data might be able to benefit from robust volume estimation models developed in (adjacent) data-rich states. While there is a reasonable amount of spatial transferability research in the transportation domain, there is a dearth of knowledge on the spatial transferability of probe-based volume estimation models. To address this gap, this paper explores spatial transferability of volume estimation models developed from data in three states (Colorado, North Carolina, and Pennsylvania). Results indicate that it is extremely important to maintain temporal consistency when attempting spatial transferability of volume estimation models. It was also found that models trained on regions with lower peak traffic volumes will limit the performance of models transferred to states with higher peak hourly traffic volumes. Corroborating findings from existing spatial transferability research on other topics, it was found that a meta-model (developed using data from multiple states) performs better than volume estimation models developed within any one of the states.
ADVANCED PROPULSION SYSTEMS↗
Chemical Kinetic Method for Active-Site Quantification in Fe-N-C Catalysts and Correlation with Molecular Probe and Spectroscopic Site-Counting Methods
Mononuclear Fe ions ligated by nitrogen (FeN x ) dispersed on nitrogen-doped carbon (Fe-N-C) serve as active centers for electrocatalytic O 2 reduction and thermocatalytic aerobic oxidations. Despite their promise as replacements for precious metals in a variety of practical applications, such as fuel cells, the discovery of new Fe-N-C catalysts has relied primarily on empirical approaches. In this context, the development of quantitative structure–reactivity relationships and benchmarking of catalysts prepared by different synthetic routes and by different laboratories would be facilitated by the broader adoption of methods to quantify atomically dispersed FeN x active centers. In the present study, we develop a kinetic probe reaction method that uses the aerobic oxidation of a model hydroquinone substrate to quantify the density of FeN x centers in Fe- N-C catalysts. The kinetic method is compared with low-temperature Mössbauer spectroscopy, CO pulse chemisorption, and electrochemical reductive stripping of NO derived from NO 2 – on a suite of Fe-N-C catalysts prepared by diverse routes and featuring either the exclusive presence of Fe as FeN x sites or the coexistence of aggregated Fe species in addition to FeN x . The FeN x site densities derived from the kinetic method correlate well with those obtained from CO pulse chemisorption and Mössbauer spectroscopy. The broad survey of Fe-N-C materials also reveals the presence of outliers and challenges associated with each site quantification approach. Furthermore, the kinetic method developed here does not require pretreatments that may alter active-site distributions nor specialized equipment beyond reaction vessels and analytical instrumentation (e.g., NMR).
36 MATERIALS SCIENCE↗
An analysis of the processing requirements of a complex perceptual-motor task
Current concerns in the assessment of mental workload are discussed, and the event-related brain potential (ERP) is introduced as a promising mental-workload index. Subjects participated in a series of studies in which they were required to perform a target acquisition task while also covertly counting either auditory or visual probes. The effects of several task-difficulty manipulations on the P300 component of the ERP elicited by the counted stimulus probes were investigated. With sufficiently practiced subjects the amplitude of the P300 was found to decrease with increases in task difficulty. The second experiment also provided evidence that the P300 is selectively sensitive to task-relevant attributes. A third experiment demonstrated a convergence in the amplitude of the P300s elicited in the simple and difficult versions of the tracking task. The amplitude of the P300 was also found to covary with the measures of tracking performance. The results of the series of three experiments illustrate the sensitivity of the P300 to the processing requirements of a complex target acquisition task. The findings are discussed in terms of the multidimensional nature of processing resources.
Kramer, A. F.↗
Resonant inelastic X-ray scattering tools to count 5 $f$ electrons of actinides and probe bond covalency
The actinides possess a complex electronic structure, making their chemical and physical properties among the least understood in the periodic table. Advanced spectroscopic tools, able to obtain deep insights into the electronic structure and binding properties of the actinides, are highly desirable. Here, we introduce two sensitive spectroscopic tools: one determines the number of localized 5f electrons on an actinide atom, and another assesses the covalent character of actinide-ligand bonding. Both tools are based on the multiplet structure present in actinide M 4 edge core-to-core resonant inelastic X-ray scattering (CC-RIXS) maps. The spectral intensity of different many-body final-state multiplets directly depends on the local many-electron ground-state symmetry including the local 5 f spin configuration. By comparing U M 4 edge CC-RIXS data for 21 U, Np, Pu and Am compounds, we demonstrate the ability to compare the number of localized 5 f electrons and bond covalency across the actinide series.
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗
The SRG/eROSITA All-Sky Survey: Constraints on the structure growth from cluster number counts
Recent advancements in methods used in wide-area surveys have demonstrated the reliability of the number density of galaxy clusters as a viable tool for precision cosmology. Beyond testing the current cosmological paradigm, cluster number counts can also be used to investigate the discrepancies currently affecting cosmological measurements. In particular, cosmological studies based on cosmic shear and other large-scale structure probes routinely find a value for the amplitude of the fluctuations in the universe S 8 = σ 8 (Ω m /0.3) 0.5 smaller than the one inferred from the primary cosmic microwave background. In this work, we investigate this tension by measuring structure evolution across cosmic time as probed by the number counts of massive halos with the first SRG/eROSITA All-Sky Survey cluster catalog in the western Galactic hemisphere, complemented with the overlapping Dark Energy Survey Year-3, Kilo-Degree Survey, and Hyper Suprime-Cam data for weak lensing mass calibration, by implementing two different parameterizations and a model-agnostic method. In the first model, we measured the cosmic linear growth index as γ = 1.19 ± 0.21, which is in tension with the standard value of γ = 0.55 but in good statistical agreement with other large-scale structure probes. The second model is a phenomenological scenario in which we rescale the linear matter power spectrum at low redshift to investigate a potential reduction of structure formation, and it provided similar results. Finally, in a third strategy, we considered a standard ΛCDM cosmology, but we separated the cluster catalog into five redshift bins, measuring the cosmological parameters in each and inferring the evolution of the structure formation, finding hints of a reduction. Interestingly, the S 8 value inferred from the number counts of the cluster eRASS1 when we add a degree of freedom to the matter power spectrum recovers the value inferred by cosmic shear studies. The observed reduction in the growth rate or systematic uncertainties associated with various measurements may account for the discrepancy in the S 8 values suggested between cosmic shear probes and eROSITA cluster number counts and Planck CMB measurements.
cosmological parameters↗
Bounds on galaxy stochasticity from halo occupation distribution modeling
The joint probability distribution of matter overdensity and galaxy counts in cells is a powerful probe of cosmology, and the extent to which variance in galaxy counts at fixed matter density deviates from Poisson shot noise is not fully understood. The lack of informed bounds on this stochasticity is currently the limiting factor in constraining cosmology with the galaxy–matter probability distribution function (PDF). We investigate stochasticity in the conditional distribution of galaxy counts along lines of sight with fixed matter density, and we present a halo occupation distribution (HOD)-based approach for obtaining plausible ranges for stochasticity parameters. To probe the high-dimensional space of possible galaxy–matter connections, we derive a set of HODs that conserve the galaxies’ linear bias and number density to produce RED M A G I C-like galaxy catalogs within the A BACUS S UMMIT suite of N -body simulations. We study the impact of individual HOD parameters and cosmology on stochasticity and perform a Monte Carlo search in HOD parameter space subject to the constraints on bias and density. In mock catalogs generated by the selected HODs, shot noise in galaxy counts spans both sub-Poisson and super-Poisson values, ranging from 80% to 133% of Poisson variance for cells with mean matter density. Nearly all of the derived HODs show a positive relationship between local matter density and stochasticity. For galaxy catalogs with higher stochasticity, modeling galaxy bias to second order is required for an accurate description of the conditional PDF of galaxy counts at fixed matter density. The presence of galaxy assembly bias also substantially extends the range of stochasticity in the super-Poisson direction. This HOD-based approach leverages degrees of freedom in the galaxy–halo connection to obtain informed bounds on nuisance model parameters and can be adapted to study other parametrizations of shot noise in galaxy counts, in particular to motivate prior ranges on stochasticity for cosmological analyses.
Britt, Dylan (ORCID:000000019905601X)↗
Return to Jupiter - Project Galileo
The mission profile, instrumentation, and mission objectives of the Galileo probe are described. Scheduled for Shuttle launch in 1986, with a boost from the Centaur upper stage, the probe will require over 2 yr to reach the Jovian system. An atmospheric entry probe will be released during Jupiter approach, and the orbiting module will relay the temperature, pressure, radio signals, spectroscopy, magnetometry, and particle counts, originating from the parachuting probe. The orbiting module will then continue on to a series of flybys and orbits around the Jovian moons by using a gravity assist from Jupiter after each pass of a moon. The orbiter will record spectrometric, radiometric, dust, visual, magnetic, and radio propagation data. The television camera is a CCD device with 640,000 diodes forming an 800 x 800 array in one square centimeter.
Johnson, T. V.↗
Nearby Molecular Hydrogen
If the gas-to-dust ratio is sufficiently uniform throughout the local interstellar medium, galaxy counts may provide a useful probe of the large scale structure of the interstellar gas. This idea substantiated by gamma ray observations led to the discovery of nearby molecular cloud complexes. The reddening studies indicate that one of them lies between 80 and 140 pc from the Sun. From CO observations, its molecular mass is estimated to be a few 1000 stellar mass units.
Lebrun, F.↗
Diffuse Reflection of Laser Light From Clouds
Laser light reflected from an aqueous suspension of particles or "cloud" with known thickness and particle size distribution defines the "cloud radiative Green's function", G. G is sensitive to cloud thickness, allowing retrieval of that important quantity. We describe a laboratory simulation of G, useful in design of an offbeam Lidar instrument for remote sensing of cloud thickness. Clouds of polystyrene microspheres suspended in water are analogous to real clouds of water droplets suspended in air. The size distribution extends from 0.5 microns to 25 microns, roughly lognormal, similar to real clouds. Density of suspended spheres is adjusted so photon mean-free-path is about 10 cm, 1000 times smaller than in real clouds. The light source is a Nd:YAG laser at 530 nm. Detectors are flux and photon-counting PMTs, with a glass probe for precise positioning. A Labview 5 VI controls position and data acquisition, via an NI Motion Control board connected to a stepper motor driving an Edmund linear slider,and a 16-channel 16-bit NI-DAQ board. The stepper motor is accurate to 10 microns. Step size is selectable. Far from the beam, the rate of exponential increase in the beam direction scales as expected from diffusion theory, linearly with cloud thickness, and inversely as the square root of the reduced optical thickness, independent of particle size. Nearer the beam the signal increases faster than exponential and depends on particle size. Results verify 3D Monte Carlo simulations that demonstrate detectability of remotely sensed offbeam returns, without filters at night, with narrow bandpass filter in day.
Cahalan, R. F.↗
First Results from the Thomson Scattering Diagnostic on the Large Plasma Device
We present the first Thomson scattering measurements of electron density and temperature in the Large Plasma Device (LAPD), a 22 m long magnetized linear plasma device at the University of California Los Angeles (UCLA). The diagnostic spectrally resolves the Doppler shift imparted on light from a frequency-doubled Nd:YAG laser when scattered by plasma electrons. A fiber array coupled to a triple-grating spectrometer is used to obtain high stray light rejection and discriminate the faint scattering signal from a much larger background. In the center of the plasma column, the measured electron density and temperature are about ne≈1.5×1013 cm−3 and Te≈ 3 eV, respectively, depending on the discharge parameters and in good agreement with Langmuir probe data. Optical design considerations to maximize photon count while minimizing alignment sensitivity are discussed in detail and compared to numerical calculations. Raman scattering off of a quartz crystal probe is used for an absolute irradiance calibration of the system.
Kaloyan, Marietta (ORCID:0000000182593038)↗
Velocity and Temperature Measurement in Supersonic Free Jets Using Spectrally Resolved Rayleigh Scattering
The flow fields of unheated, supersonic free jets from convergent and convergent-divergent nozzles operating at M = 0.99, 1.4, and 1.6 were measured using spectrally resolved Rayleigh scattering technique. The axial component of velocity and temperature data as well as density data obtained from a previous experiment are presented in a systematic way with the goal of producing a database useful for validating computational fluid dynamics codes. The Rayleigh scattering process from air molecules provides a fundamental means of measuring flow properties in a non-intrusive, particle free manner. In the spectrally resolved application, laser light scattered by the air molecules is collected and analyzed using a Fabry-Perot interferometer (FPI). The difference between the incident laser frequency and the peak of the Rayleigh spectrum provides a measure of gas velocity. The temperature is measured from the spectral broadening caused by the random thermal motion and density is measured from the total light intensity. The present point measurement technique uses a CW laser, a scanning FPI and photon counting electronics. The 1 mm long probe volume is moved from point to point to survey the flow fields. Additional arrangements were made to remove particles from the main as well as the entrained flow and to isolate FPI from the high sound and vibration levels produced by the supersonic jets. In general, velocity is measured within +/- 10 m/s accuracy and temperature within +/- 10 K accuracy.
Panda, J.↗
Evidence for Natural Variability in Marine Stratocumulus Cloud Properties Due to Cloud-Aerosol
In this study, aircraft observations from the Interdisciplinary Remotely-Piloted Aircraft Studies (CIRPAS) Twin Otter are used to characterize the variability in drizzle, cloud, and aerosol properties associated with cloud rifts and the surrounding solid clouds observed off the coast of California. A flight made on 16 July 1999 provided measurements directly across an interface between solid and rift cloud conditions. Aircraft instrumentation allowed for measurements of aerosol, cloud droplet, and drizzle spectra. CCN concentrations were measured in addition to standard thermodynamic variables and the winds. A Forward Scatter Spectrometer Probe (FSSP) measured size distribution of cloud-sized droplets. A Cloud Imaging Probe (CIP) was used to measure distributions of drizzle-sized droplets. Aerosol distributions were obtained from a Cloud Aerosol Scatterprobe (CAS). The CAS probe measured aerosols, cloud droplets and drizzle-sized drops; for this study. The CAS probe was used to measure aerosols in the size range of 0.5 micron - 1 micron. Smaller aerosols were characterized using an Ultrafine Condensation Particle Counter (CPC) sensor. The CPC was used to measure particles with diameters greater than 0.003 micron. By subtracting different count concentrations measured with the CPC, this probe was capable of identifying ultrafine particles those falling in the size range of 3 nanometers - 7 nanometers that are believed to be associated with new particle production.
Albrecht, Bruce↗
Toward streaked collective Thomson scattering measurements on an extreme ultraviolet plasma light source
Here we show through forward modeling calculations that streaked collective Thomson scattering measurements are feasible on laser-produced tin plasmas generated under conditions relevant for extreme ultraviolet lithography. Using a 532 nm probe laser beam, the feasibility of simultaneous measurements of electron plasma wave (EPW) and ion acoustic wave (IAW) spectra is investigated. Absolute photon counts for laser scattering off both waves are calculated. Probe laser electron heating and bremsstrahlung background radiation effects are accounted for. While a large spatiotemporal region can be successfully probed based on the IAW feature, only one measurement location can be accessed through the EPW as a result of the low signal to noise ratio. A portable/traveling tabletop system is proposed.
47 OTHER INSTRUMENTATION↗
Beyond the 3rd moment: a practical study of using lensing convergence CDFs for cosmology with DES Y3
ABSTRACT Widefield surveys probe clustered scalar fields – such as galaxy counts, lensing potential, etc. – which are sensitive to different cosmological and astrophysical processes. Constraining such processes depends on the statistics that summarize the field. We explore the cumulative distribution function (CDF) as a summary of the galaxy lensing convergence field. Using a suite of N-body light-cone simulations, we show the CDFs’ constraining power is modestly better than the second and third moments, as CDFs approximately capture information from all moments. We study the practical aspects of applying CDFs to data, using the Dark Energy Survey (DES Y3) data as an example, and compute the impact of different systematics on the CDFs. The contributions from the point spread function and reduced shear approximation are $\lesssim 1~{{\ \rm per\ cent}}$ of the total signal. Source clustering effects and baryon imprints contribute 1–10 per cent. Enforcing scale cuts to limit systematics-driven biases in parameter constraints degrade these constraints a noticeable amount, and this degradation is similar for the CDFs and the moments. We detect correlations between the observed convergence field and the shape noise field at 13σ. The non-Gaussian correlations in the noise field must be modelled accurately to use the CDFs, or other statistics sensitive to all moments, as a rigorous cosmology tool.
79 ASTRONOMY AND ASTROPHYSICS↗
Cloud Thickness from Diffusion of Lidar Pulses in Clouds
Measurements of the distribution of reflected light from a laser beam incident on an aqueous suspension of particles or "cloud" with known thickness and particle size distribution are reported. The distribution is referred to as the "cloud radiative Green's function", G. In the diffusion domain, G is sensitive to cloud thickness, allowing that important quantity to be retrieved. The goal of the laboratory simulation is to provide preliminary estimates of sensitivity of G to cloud thickness,for use in the optimal design of an offbeam Lidar instrument for remote sensing of cloud thickness (THOR, Thickness from Offbeam Returns). These clouds of polystyrene microspheres suspended in water are analogous to real clouds of water droplets suspended in air. The microsphere size distribution is roughly lognormal, from 0.5 microns to 25 microns, similar to real clouds. Density of suspended spheres is adjusted so mean-free-path of visible photons is about 10 cm, approximately 1000 times smaller than in real clouds. The light source is a ND:YAG laser at 530 nm. Detectors are flux and photon-counting Photomultiplier Tube (PMTS), with a glass probe for precise positioning. A Labview 5 VI controls positioning, and data acquisition, via an NI Motion Control board connected to a stepper motor driving an Edmund linear slider, and a 16-channel 16-bit NI-DAQ board. The stepper motor is accurate to 10 microns, and step size is selectable from the VI software. Far from the incident beam, the rate of exponential increase as the direction of the incident beam is approached scales as expected from diffusion theory, linearly with the cloud thickness, and inversely as the square root of the reduced optical thickness, and is independent of particle size. Near the beam the signal begins to increase faster than exponential, due to single and low-order scattering near the backward direction, and here the distribution depends on particle size. Results are being used to verify 3D Monte Carlo radiative transfer simulations, used to estimate signal-to-noise ratios for remotely sensed off beam returns, for both homogeneous and inhomogeneous clouds. Signal-to-noise estimates show that unfiltered observations are straight forward at night, while narrow band pass filters are being studied for day.
Cahalan, Robert F.↗
X-ray Mapping of Terrestrial and Extraterrestrial Materials Using the Electron Microprobe
Lunar samples returned from the Apollo program motivated development of the Bence-Albee algorithm for the rapid and accurate analysis of lunar materials, and established interlaboratory comparability through its common use. In the analysis of mineral and rock fragments it became necessary to combine micro- and macroscopic analysis by coupling electron-probe microanalysis (EPMA) with automated stage point counting. A coarse grid that included several thousand points was used, and initially wavelength-dispersive (WDS) and later energydispersive (EDS) data were acquired at discrete stage points using approx. 5 sec count times. A approx 50 micrometer beam diameter was used for WDS and up to 500 micrometer beam diameter for EDS analysis. Average analyses of discretely sampled phases were coupled with the point count data to calculate the bulk composition using matrix algebra. Use of a defocused beam resulted in a contribution from multiple phases to each analytical point, and the analytical data were deconvolved relative to end-member phase chemistry on the fly. Impressive agreement was obtained between WDS and EDS measurements as well as comparison with bulk chemistry obtained by other methods. In the 30 years since these methods were developed, significant improvements in EPMA automation and computer processing have taken place. Digital beam control allows routine collection of x-ray maps by EDS, and stage mapping for WDS is conducted continuously at slew speed and incrementally by sampling at discrete points. Digital pulse processing in EDS systems has significantly increased the throughput for EDS mapping, and the ongoing development of Si-drift detector systems promises mapping capabilities rivaling WDS systems. Spectrum imaging allows a data cube of EDS spectra to be acquired and sophisticated processing of the original data is possible using matrix algebra techniques. The study of lunar and meteoritic materials includes the need to conveniently: (1) Characterize the sample at microscopic and macroscopic scales with relatively high sensitivity, (2) Determine the modal abundance of minerals, and (3) Identify and relocate discrete features of interest in terms of size and chemistry. The coupled substitution of cations in minerals can result in significant variation in mineral chemistry, but at similar average Z, leading to poor backscattered-electron (BSE) contrast discrimination of mineralogy. It is necessary to discriminate phase chemistry at both the trace element level and the major element level. To date, the WDS of microprobe systems is preferred for mapping due to high throughput and the ability to obtain the necessary intensity to discriminate phases at both trace and major element concentrations. It is desirable to produce fully quantitative compositional maps of geological materials, which requires the acquisition of k-ratio maps that are background and dead-time corrected, and which have been corrected by phi(delta z> or an equivalent algorithm at each pixel. To date, turnkey systems do not allow the acquisition of k-ratio maps and the rigorous correction in this manner. X-ray maps of a chondrule from the Ourique meteorite, and a comb-layered xenolith from the San Francisco volcanic field, have been analyzed and processed to extract phase information. The Ourique meteorite presents a challenge due to relatively low BSE contrast, and has been studied using spectrum imaging. X-ray maps for Si, Mg, and FeK(alpha) were used to produce RGB images. The xenolith sample contains sector-zoned augite, olivine, plagioclase, and basaltic glass. X-ray maps were processed using Lispix and ImageJ software to produce mineral phase maps. The x-ray maps for Mg, Ca, and Ti were used with traceback to generate binary images that were converted to RGB images. These approaches are successful in discriminating phases, but it is desirable to achieve the methods that were used on lunar samples 30 years ago on current microprobe systems. Curnt research includes x-ray mapping analysis of the Dalgety Downs chondrite by micro x-ray fluorescence and spectrum imaging, in collaboration with Kenny Witherspoon of IXRF Systems and Dale Newbury of NIST.
Carpenter, P.↗
Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes
Two rapid in situ enumeration methods using fluorescent probes were used to assess the physiological activities of Klebsiella pneumoniae biofilms on stainless steel. Fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and rhodamine 123 (Rh 123), were chosen to perform this study. CTC is a soluble redox indicator which can be reduced by respiring bacteria to fluorescent CTC-formazan crystals. Rh 123 is incorporated into bacteria with respect to cellular proton motive force. The intracellular accumulation of these fluorescent dyes can be determined using epifluorescence microscopy. The results obtained with these two fluorescent probes in situ were compared to the plate count (PC) and in situ direct viable count (DVC) methods. Viable cell densities within biofilms determined by the three in situ methods were comparable and always showed approximately 2-fold higher values than those obtained with the PC method. As an additional advantage, the results were observed after 2 h, which was shorter than the 4 h incubation time required for the DVC method and 24 h for colony formation. The results indicate that staining with CTC and Rh 123 provides rapid information regarding cell numbers and physiological activities of bacteria within biofilms.
NASA Discipline Number 04-10↗