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At least 19 records

Columella cells revisited: novel structures, novel properties, and a novel gravisensing model

A hundred years of research has not produced a clear understanding of the mechanism that transduces the energy associated with the sedimentation of starch-filled amyloplast statoliths in root cap columella cells into a growth response. Most models postulate that the statoliths interact with microfilaments (MF) to transmit signals to the plasma membrane (or ER), or that sedimentation onto these organelles produces the signals. However, no direct evidence for statolith-MF links has been reported, and no asymmetric structures of columella cells have been identified that might explain how a root turned by 90 degrees knows which side is up. To address these and other questions, we have (1) quantitatively examined the effects of microgravity on the size, number, and spatial distribution of statoliths; (2) re-evaluated the ultrastructure of columella cells in high-pressure frozen/freeze-substituted roots; and (3) followed the sedimentation dynamics of statolith movements in reoriented root tips. The findings have led to the formulation of a new model for the gravity-sensing apparatus of roots, which envisages the cytoplasm pervaded by an actin-based cytoskeletal network. This network is denser in the ER-devoid central region of the cell than in the ER-rich cell cortex and is coupled to receptors in the plasma membrane. Statolith sedimentation is postulated to disrupt the network and its links to receptors in some regions of the cell cortex, while allowing them to reform in other regions and thereby produce a directional signal.

NASA Discipline Cell Biology

Proteins with Novel Structure, Function and Dynamics

Recently, a small enzyme that ligates two RNA fragments with the rate of 10(exp 6) above background was evolved in vitro (Seelig and Szostak, Nature 448:828‐831, 2007). This enzyme does not resemble any contemporary protein (Chao et al., Nature Chem. Biol. 9:81‐83, 2013). It consists of a dynamic, catalytic loop, a small, rigid core containing two zinc ions coordinated by neighboring amino acids, and two highly flexible tails that might be unimportant for protein function. In contrast to other proteins, this enzyme does not contain ordered secondary structure elements, such as alpha‐helix or beta‐sheet. The loop is kept together by just two interactions of a charged residue and a histidine with a zinc ion, which they coordinate on the opposite side of the loop. Such structure appears to be very fragile. Surprisingly, computer simulations indicate otherwise. As the coordinating, charged residue is mutated to alanine, another, nearby charged residue takes its place, thus keeping the structure nearly intact. If this residue is also substituted by alanine a salt bridge involving two other, charged residues on the opposite sides of the loop keeps the loop in place. These adjustments are facilitated by high flexibility of the protein. Computational predictions have been confirmed experimentally, as both mutants retain full activity and overall structure. These results challenge our notions about what is required for protein activity and about the relationship between protein dynamics, stability and robustness. We hypothesize that small, highly dynamic proteins could be both active and fault tolerant in ways that many other proteins are not, i.e. they can adjust to retain their structure and activity even if subjected to mutations in structurally critical regions. This opens the doors for designing proteins with novel functions, structures and dynamics that have not been yet considered.

Proteins

A Rational Approach to Determine Minimum Strength Thresholds in Novel Structural Materials

Design of safe and survivable structures requires the availability of guaranteed minimum strength thresholds for structural materials to enable a meaningful comparison of strength requirement and available strength. This paper develops a procedure for determining such a threshold with a desired degree of confidence, for structural materials with none or minimal industrial experience. The problem arose in attempting to use a new, highly weight-efficient structural load tendon material to achieve a lightweight super-pressure balloon. The developed procedure applies to lineal (one dimensional) structural elements. One important aspect of the formulation is that it extrapolates to expected probability distributions for long length specimen samples from some hypothesized probability distribution that has been obtained from a shorter length specimen sample. The use of the developed procedure is illustrated using both real and simulated data.

Schur, Willi W.

TriTruss: A New and Novel Structural Concept Enabling Modular Space Telescopes and Space Platforms

Modular structures that can be assembled on-orbit will be the backbone for all future persistent missions, including in-space assembled telescopes and platforms for science and communications. The TriTruss is a new and innovative structural module that has been conceived by researchers at the NASA Langley Research Center for platform and telescope applications. Some of the innovative features of the TriTruss include: very compact packaging for launch, the possibility of staged packaging, simple robotic deployment, ease of embedding payload components, an innovative structural connector that has linear structural performance, ease of module-to-module robotic assembly, design versatility, and ease of customizing its design for specific applications. This paper will introduce the TriTruss concept and describe how it can serve as the foundation for many different mission applications, in particular, a 20-meter diameter large space telescope and a beam-type platform that can host a variety of payloads and instruments. The geometry of the TriTruss will be described and the various truss design variables (such as truss depth, member diameter, material modulus, etc.) and each of their impacts on the truss performance will be illustrated. The TriTruss can be mapped to a variety of structural forms, such as beams, two-dimensional platforms and filled curved apertures (for antennas and telescopes), and examples will be illustrated. The TriTruss lends itself to a large variety of packaging schemes; the structural concepts associated with packaging and deployment will be described, as well as the means for robotically deploying TriTruss modules and locking them into their final configuration. TriTruss module-to-TriTruss module robotic assembly operations will also be described. Equations will be presented to structurally size TriTruss modules, such that when assembled into the final persistent platform, the platform achieves a desired level of global structural performance. A status of the TriTruss development will also be presented. This material will cover design and fabrication of TriTruss hardware for platform and telescope applications as well as structural testing of that hardware (the struts, connectors and platforms). Robotic assembly of TriTruss modules is also being performed, and the results of those tests will be summarized.

Doggett, William

Reactivity of pi-complexes of Ti, V, and Nb towards dithioacetic acid: Synthesis and structure of novel metal sulfur-containing complexes

In order to use sulfur-containing resources economically and with minimal environmental damage, it is important to understand the desulfurization processes. Hydrodesulfurization, for example, is carried out on the surface of a heterogeneous metal sulfide catalyst. Studies of simple, soluble inorganic systems provide information regarding the structure and reactivity of sulfur-containing compounds with metal complexes. Further, consistent with recent trends in materials chemistry, many model compounds warrant further study as catalyst precursors. The reactivity of low-valent organometallic sandwich pi-complexes toward dithiocarboxylic acids is described. For example, treatment of bisbenzene vanadium with CH3CSSH affords a divanadium tetrakis(dithioacetate) complex. The crystallographically determined V-V bond distance, 2.800(2), is nearly the same as the V-V bond distance in a V(mu-nu squared-S2)2V' unit in the mineral patonite (VS4)n. The stability of the V2S4 core in the dimer is demonstrated by evidence of V2S4(+) in the mass spectrum (70 eV, solid probe) of the vanadium dimer. Several other systems relevant to HDS catalysis are also discussed.

Duraj, Stan A.

Mechanical Model Development for Composite Structural Supercapacitors

Novel composite structural supercapacitor concepts have recently been developed as a means both to store electrical charge and to provide modest mechanical load carrying capability. Double-layer composite supercapacitors are often fabricated by impregnating a woven carbon fiber fabric, which serves as the electrodes, with a structural polymer electrolyte. Polypropylene or a glass fabric is often used as the separator material. Recent research has been primarily limited to evaluating these composites experimentally. In this study, mechanical models based on the Multiscale Generalized Method of Cells (MSGMC) were developed and used to calculate the shear and tensile properties and response of two composite structural supercapacitors from the literature. The modeling approach was first validated against traditional composite laminate data. MSGMC models for composite supercapacitors were developed, and accurate elastic shear/tensile properties were obtained. It is envisioned that further development of the models presented in this work will facilitate the design of composite components for aerospace and automotive applications and can be used to screen candidate constituent materials for inclusion in future composite structural supercapacitor concepts.

Micromechanics

Efficient Design and Analysis of Lightweight Reinforced Core Sandwich and PRSEUS Structures

Design, analysis, and sizing methods for two novel structural panel concepts have been developed and incorporated into the HyperSizer Structural Sizing Software. Reinforced Core Sandwich (RCS) panels consist of a foam core with reinforcing composite webs connecting composite facesheets. Boeing s Pultruded Rod Stitched Efficient Unitized Structure (PRSEUS) panels use a pultruded unidirectional composite rod to provide axial stiffness along with integrated transverse frames and stitching. Both of these structural concepts are ovencured and have shown great promise applications in lightweight structures, but have suffered from the lack of efficient sizing capabilities similar to those that exist for honeycomb sandwich, foam sandwich, hat stiffened, and other, more traditional concepts. Now, with accurate design methods for RCS and PRSEUS panels available in HyperSizer, these concepts can be traded and used in designs as is done with the more traditional structural concepts. The methods developed to enable sizing of RCS and PRSEUS are outlined, as are results showing the validity and utility of the methods. Applications include several large NASA heavy lift launch vehicle structures.

Bednarcyk, Brett A.

Structural Characterization of a TriTruss Module

The TriTruss is a novel structural module developed by researchers at NASA Langley Research Center (LaRC) that can be used in space to assemble large support structures for a variety of applications. One such application is the metering truss or primary mirror backbone support structure of an In-Space Assembled Telescope (iSAT). For the iSAT application, the TriTruss will be supporting mirror segments, payloads, and instruments, all of which require the TriTruss to have a high stiffness. Structural characterization from testing and analysis is needed to ensure the integrity of the struts that make up a TriTruss module is maintained when subjected to loads representative of the application. The test setup and loads applied to the TriTruss module as well as the analytical methods used to predict the response of the structure under conditions representative of those implemented during testing will be discussed. Also, the results obtained from testing and analysis will be summarized. The goal for the characterization study was to achieve a correlation within 10% between the test data and the analysis. Overall, the correlation varied for the struts with a few struts still having a larger error margin after further studies were conducted to improve the correlation through additional analysis.

Characterization

Macromolecular differentiation of Golgi stacks in root tips of Arabidopsis and Nicotiana seedlings as visualized in high pressure frozen and freeze-substituted samples

The plant root tip represents a fascinating model system for studying changes in Golgi stack architecture associated with the developmental progression of meristematic cells to gravity sensing columella cells, and finally to "young" and "old", polysaccharide-slime secreting peripheral cells. To this end we have used high pressure freezing in conjunction with freeze-substitution techniques to follow developmental changes in the macromolecular organization of Golgi stacks in root tips of Arabidopsis and Nicotiana. Due to the much improved structural preservation of all cells under investigation, our electron micrographs reveal both several novel structural features common to all Golgi stacks, as well as characteristic differences in morphology between Golgi stacks of different cell types. Common to all Golgi stacks are clear and discrete differences in staining patterns and width of cis, medial and trans cisternae. Cis cisternae have the widest lumina (approximately 30 nm) and are the least stained. Medial cisternae are narrower (approximately 20 nm) and filled with more darkly staining products. Most trans cisternae possess a completely collapsed lumen in their central domain, giving rise to a 4-6 nm wide dark line in cross-sectional views. Numerous vesicles associated with the cisternal margins carry a non-clathrin type of coat. A trans Golgi network with clathrin coated vesicles is associated with all Golgi stacks except those of old peripheral cells. It is easily distinguished from trans cisternae by its blebbing morphology and staining pattern. The zone of ribosome exclusion includes both the Golgi stack and the trans Golgi network. Intercisternal elements are located exclusively between trans cisternae of columella and peripheral cells, but not meristematic cells. In older peripheral cells only trans cisternae exhibit slime-related staining. Golgi stacks possessing intercisternal elements also contain parallel rows of freeze-fracture particles in their trans cisternal membranes. We propose that intercisternal elements serve as anchors of enzyme complexes involved in the synthesis of polysaccharide slime molecules to prevent the complexes from being dragged into the forming secretory vesicles by the very large slime molecules. In addition, we draw attention to the similarities in composition and apparent site of synthesis of xyloglucans and slime molecules.

NASA Program Space Biology

Tailored Wingbox Structures through Additive Manufacturing: A Summary of Ongoing Research at NASA LaRC

The use of wingbox structural design for improved performance (i.e., fuel burn reduction) of subsonic transports is driven by two trends: reduced structural weight and increased wingspan. These two trends are in direct competition, as the increased span will exacerbate the structural reaction to aerodynamic loading, and the reduced structural weight will nominally weaken the aircraft’s ability to handle this response. Novel structural configurations, enabled by recent improvements in manufacturing, may be critical toward bridging this gap.This paper summarizes pertinent activities at the NASA Langley Research Center in terms of additive manufacturing of metallic wing structures and substructures. Numerical design optimization activities are summarized as well, in order to understand where on a wingbox an additively-manufactured part may be useful and the way in which that part beneficially impacts the flight physics. The paper concludes with a discussion of how these two research paths may be better married in order to fully integrate both the benefits and realistic limitations of additive manufacturing and numerical structural design.

Stanford, Bret. K.

Structural concepts for future space transportation orbiters

Two structural concepts for a fully reusable STS orbiter are compared with respect to weight and the ability to satisfy structural goals. The cryogenic propellant tanks for both concepts use welded, blade-stiffened aluminum skins for leak-free containment and compatibility with LOX and LH2. The tanks are sized primarily by pressure, although some areas are sized by bending and fracture mechanics considerations. The tank concept is regarded as state-of-the-art, even though it requires a closed cell foam insulation with a dry air purge to prevent air liquefaction and ice formation. It is noted that the foam will require testing to verify its design life for use in the STS. One concept has nonintegral tanks suspended inside an insulated aluminum airframe and thrust structure. Here, a durable thermal protection system external insulation is mechanically attached to the airframe, allowing a buckled skin design. The other concept uses a novel structural arrangement consisting of a separate tank/thrust structure that supports a hot advanced-carbon-carbon aeroshell structure.

Taylor, A. H.

A tri-state optical switch for local area network communications

This novel structure is a heterojunction phototransistor which can be used as an emitter-detector, and when placed in a quiescent mode, the device becomes a passive transmitter. By varying the voltage bias, this novel device will switch between all three modes of operation. Such a device has broad application in network environments with operation speeds of less than 50 MHz and distances of less than 1 km, e.g. automobiles, airplanes, and intra-instrumentation. During this period, the emission mode for this device was studied and mathematically modeled.

Simms, Garfield

PRSEUS Structural Concept Development

A lighter, more robust airframe is one of the key technological advancements necessary for the successful launch of any large next-generation transport aircraft. Such a premise dictates that considerable improvements beyond current state-of-the-art aluminum structures is needed, and that improvements of this magnitude will require an extensive use of composite materials that are not only lightweight, but also economical to produce. To address this challenge, researchers at NASA and The Boeing Company are developing a novel structural concept called the Pultruded Rod Stitched Efficient Unitized Structure (PRSEUS) under the Environmentally Responsible Aviation (ERA) Project. It is an integrally stiffened panel concept that is stitched together and designed to maintain residual load-carrying capabilities under a variety of damage scenarios. In addition to improved structural performance, an important facet of this unique arrangement of stitched carbon fibers is its innovative manufacturing method that has the potential to lower fabrication costs by eliminating fasteners and autoclave cures. The rationale and development status for this new approach forms the basis of the work described in this paper. The test specimens described herein were fabricated, or are currently being fabricated, by The Boeing Company, while the structural analyses and testing tasks are being performed by NASA and Boeing personnel.

Velicki, Alex

Airborne rotary air separator study

Several air breathing propulsion concepts for future earth-to-orbit transport vehicles utilize air collection and enrichment, and subsequent storage of liquid oxygen for later use in the vehicle emission. Work performed during the 1960's established the feasibility of substantially reducing weight and volume of a distillation type air separator system by operating the distillation elements in high 'g' fields obtained by rotating the separator assembly. This contract studied the capability test and hydraulic behavior of a novel structured or ordered distillation packing in a rotating device using air and water. Pressure drop and flood points were measured for different air and water flow rates in gravitational fields of up to 700 g. Behavior of the packing follows the correlations previously derived from tests at normal gravity. The novel ordered packing can take the place of trays in a rotating air separation column with the promise of substantial reduction in pressure drop, volume, and system weight. The results obtained in the program are used to predict design and performance of rotary separators for air collection and enrichment systems of interest for past and present concepts of air breathing propulsion (single or two-stage to orbit) systems.

Acharya, A.