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At least 19 records

Mechanical stimulation improves tissue-engineered human skeletal muscle

Human bioartificial muscles (HBAMs) are tissue engineered by suspending muscle cells in collagen/MATRIGEL, casting in a silicone mold containing end attachment sites, and allowing the cells to differentiate for 8 to 16 days. The resulting HBAMs are representative of skeletal muscle in that they contain parallel arrays of postmitotic myofibers; however, they differ in many other morphological characteristics. To engineer improved HBAMs, i.e., more in vivo-like, we developed Mechanical Cell Stimulator (MCS) hardware to apply in vivo-like forces directly to the engineered tissue. A sensitive force transducer attached to the HBAM measured real-time, internally generated, as well as externally applied, forces. The muscle cells generated increasing internal forces during formation which were inhibitable with a cytoskeleton depolymerizer. Repetitive stretch/relaxation for 8 days increased the HBAM elasticity two- to threefold, mean myofiber diameter 12%, and myofiber area percent 40%. This system allows engineering of improved skeletal muscle analogs as well as a nondestructive method to determine passive force and viscoelastic properties of the resulting tissue.

Non-NASA Center↗

Environmental Barrier Coating Fracture, Fatigue and High-Heat-Flux Durability Modeling and Stochastic Progressive Damage Simulation

Advanced environmental barrier coatings will play an increasingly important role in future gas turbine engines because of their ability to protect emerging light-weight SiC/SiC ceramic matrix composite (CMC) engine components, further raising engine operating temperatures and performance. Because the environmental barrier coating systems are critical to the performance, reliability and durability of these hot-section ceramic engine components, a prime-reliant coating system along with established life design methodology are required for the hot-section ceramic component insertion into engine service. In this paper, we have first summarized some observations of high temperature, high-heat-flux environmental degradation and failure mechanisms of environmental barrier coating systems in laboratory simulated engine environment tests. In particular, the coating surface cracking morphologies and associated subsequent delamination mechanisms under the engine level high-heat-flux, combustion steam, and mechanical creep and fatigue loading conditions will be discussed. The EBC compostion and archtechture improvements based on advanced high heat flux environmental testing, and the modeling advances based on the integrated Finite Element Analysis Micromechanics Analysis Code/Ceramics Analysis and Reliability Evaluation of Structures (FEAMAC/CARES) program will also be highlighted. The stochastic progressive damage simulation successfully predicts mud flat damage pattern in EBCs on coated 3-D specimens, and a 2-D model of through-the-thickness cross-section. A 2-parameter Weibull distribution was assumed in characterizing the coating layer stochastic strength response and the formation of damage was therefore modeled. The damage initiation and coalescence into progressively smaller mudflat crack cells was demonstrated. A coating life prediction framework may be realized by examining the surface crack initiation and delamination propagation in conjunction with environmental degradation under high-heat-flux and environment load test conditions.

Mechanical creep and fatigue loading condition↗

Pattern Identification - A Foundation for Research in the Emphasis of Design Patterns in Systems Engineering and Knowledge Capture

Pattern Language describes the morphology and functionality of a system in the absence of design particulars. Harnessing this capability will provide the Systems Engineering discipline a means of managing the development of increasingly complex systems with increasingly distributed design teams while capturing and retaining knowledge for future generations. Pattern Language is a syntax for describing, and structurally relating, design patterns. Design patterns contextually describe the application of domain knowledge in the engineered solution to the force balance problem. The parallels between pattern recognition and application, as a fundamental stage of human learning, and pattern observation within a complex system, suggests pattern language may be a valuable tool in the capture and dissemination of knowledge. Pattern application has enjoyed considerable study over the last several decades, however much of this work has focused on the replication of design particulars. This work returns to the roots of Pattern Language and explores the utility of patterns as an architectural description and guide, and knowledge capture method, for complex system development beginning with the identification of a time proven design pattern.

Russell, Samuel P.↗

Electrospraying and Electrospinning of Polymers for Biomedical Applications. Poly(Lactic-Co-Glycolic Acid) and Poly(Ethylene-Co-Vinylacetate)

Significant opportunities exist for the processing of polymers (homopolymers and blends) using electric fields. Specific attention is given here to electrospinning, but we note that electroaerosol formation and field-modulated film casting represent additional processing options. Of particular interest is the ability to generate polymer fibers of sub-micron dimensions using electrospinning, down to about 0.05 microns (50 nm), a size range that has been traditionally difficult to access. In our work, poly(lactic-co-glycolic acid), PLA/PGA, poly(lactic acid) PLA, and poly(ethylene-co-vinylacetate) (PEVA) have been deposited from solutions in methylene chloride or chloroform by electrospraying or electrospinning to afford morphologically tailored materials for tissue engineering and related applications. Low solution concentrations tend to favor electrostatic spraying ('electro-aerosolization') while higher concentrations lead to spinning on fibrous mats. Preliminary observations of muscle cell growth on PLA electrospun mats are reported.

Stitzel, Joel D.↗

The Isothermal Dendritic Growth Experiment

The growth of dendrites is one of the commonly observed forms of solidification encountered when metals and alloys freeze under low thermal gradients, as occurs in most casting and welding processes. In engineering alloys, the details of the dendritic morphology directly relates to important material responses and properties. Of more generic interest, dendritic growth is also an archetypical problem in morphogenesis, where a complex pattern evolves from simple starting conditions. Thus, the physical understanding and mathematical description of how dendritic patterns emerge during the growth process are of interest to both scientists and engineers. The Isothermal Dendritic Growth Experiment (IDGE) is a basic science experiment designed to measure, for a fundamental test of theory, the kinetics and morphology of dendritic growth without complications induced by gravity-driven convection. The IDGE, a collaboration between Rensselaer Polytechnic Institute, in Troy NY, and NASA's Lewis Research Center (LeRC) was developed over a ten year period from a ground-based research program into a space flight experiment. Important to the success of this flight experiment was provision of in situ near-real-time teleoperations during the spaceflight experiment.

Glicksman, M. E.↗

Hierarchical Velocity Structure in the Core of Abell 2597

We present XMM-Newton RGS and EPIC data of the putative cooling flow cluster Abell 2597. Velocities of the low-ionization emission lines in the spectrum are blue shifted with respect to the high-ionization lines by 1320 (sup +660) (sub -210) kilometers per second, which is consistent with the difference in the two peaks of the galaxy velocity distribution and may be the signature of bulk turbulence, infall, rotation or damped oscillation in the cluster. A hierarchical velocity structure such as this could be the direct result of galaxy mergers in the cluster core, or the injection of power into the cluster gas from a central engine. The uniform X-ray morphology of the cluster, the absence of fine scale temperature structure and the random distribution of the the galaxy positions, independent of velocity, suggests that our line of sight is close to the direction of motion. These results have strong implications for cooling flow models of the cluster Abell 2597. They give impetus to those models which account for the observed temperature structure of some clusters using mergers instead of cooling flows.

Still, Martin↗

Strengthening Precipitate Morphologies Fully Quantified in Advanced Disk Superalloys

Advanced aviation gas turbine engines will require disk superalloys that can operate at higher temperatures and stresses than current conditions. Such applications will be limited by the tensile, creep, and fatigue mechanical properties of these alloys. These mechanical properties vary with the size, shape, and quantity of the gamma precipitates that strengthen disk superalloys. It is therefore important to quantify these precipitate parameters and relate them to mechanical properties to improve disk superalloys. Favorable precipitate morphologies and practical processing approaches to achieve them can then be determined. A methodology has been developed at the NASA Lewis Research Center to allow the comprehensive quantification of the size, shape, and quantity of all types of gamma precipitates.

Gabb, Timothy P.↗

Spectral filtering for plant production

Both plants and animals have one general commonality in their perception of light. They both are sensitive primarily to the 400 to 700 nm wavelength portion of the electromagnetic spectrum. This is referred to as the visible spectrum for animals and as the photosynthetically active radiation (PAR) spectrum for plants. Within this portion of the spectrum, animals perceive colors. Relatively recently it has been learned that within this same spectral range plants also demonstrate varying responses at different wavelengths, somewhat analogous to the definition of various colors at specific wavelengths. Although invisible to the human eye, portions of the electromagnetic spectrum on either side of the visible range are relatively inactive photosynthetically but have been found to influence important biological functions. These portions include the ultraviolet (UV approximately equal to 280-400 nm) and the far-red (FR approximately equal to 700-800 nm). The basic photoreceptor of plants for photosynthesis is chlorophyll. It serves to capture radiant energy which combined with carbon dioxide and water produces oxygen and assimulated carbon, used for the synthesis of cell wall polysaccarides, proteins, membrane lipids and other cellular constituents. The energy and carbon building blocks of photosynthesis sustain growth of plants. On the other hand, however, there are other photoreceptors, or pigments, that function as signal transducers to provide information that controls many physiological and morphological responses of how a plant grows. Known photomorphogenic receptors include phytochrome (the red/far-red sensor in the narrow bands of 655-665 nm and 725-735 nm ranges, respectively) and 'cryptochrome' (the hypothetical UV-B sensor in the 280-320 nm range). Since the USDA team of W. L. Butler, S. B. Hendricks, H. A. Borthwick, H. A. Siegleman and K. Norris in Beltsville, MD detected by spectroscopy, extracted and identified phytochrome as a protein in the 1950's, many other investigators have found evidence of its control functions in plants. Considerably less, however, is known about the yet non-isolated cryptochrome. The information-transferring roles of photoreceptors in plants at specific spectral ranges quite naturally stimulated plant scientists and engineers to consider physically manipulating light to achieve desired physiological and morphological characteristics. One way to manipulate light is to filter it through materials that selectively transmit portions of the sun's spectrum in and near the PAR range.

Young, Roy E.↗

Effect of Aromaticity on Soot Formation: A Reactive Molecular Dynamics Study

This study investigates the formation of soot from n-dodecane and its blend with m-xylene under pyrolytic and low-oxygen conditions with reactive molecular dynamics (MD) simulations. ReaxFF force field is used to simulate the interactions of fuel and oxygen molecules at engine-relevant conditions (2500-3500 K and 70-75 bar). Fuel molecules with carbon atom density of 0.03 g/cm 3 are dispersed into a cube and constant temperature simulations are carried out with a Nosé-Hoover thermostat. Pyrolysis of n-dodecane and the chemical pathway towards the formation of aromatic hydrocarbons are investigated. The initial pyrolysis rate of n-dodecane is observed to be slightly higher with the addition of aromatic compounds and oxygen. While the first aromatics formed from the decomposed n-dodecane are phenyl radicals at 2500 K, decomposition of the aliphatic fuel compound is observed to be necessary to build a carbon cluster, and eventually soot. Morphological properties of the created soot particles are also analyzed. The maturity, size and aromatics content of the soot particle are slightly affected with the addition of m-xylene and oxygen at 3500 K within the 3 ns simulation time possibly due the formation of larger and more condensed aromatic rings, resulting in larger soot molecules. The outcomes of this study provide important insights into the formation mechanism of soot and its morphological characteristics depending on varying fuel concentrations at pyrolysis and low oxygen engine conditions. Results of this analysis will further contribute to the understanding of contrail formation on generated soot particles originated from the combustion of different chemical components with particle chemistry matching companion experiments.

soot formation↗

Decision Space Modeling: Trade Space Ontology

As the National Aeronautics and Space Administration (NASA) works to develop a crewed Moon to Mars Architecture, it is dealing with a large decision space consisting of the overlay of human exploration architectures for both the Moon and for Mars. Efforts are underway to enable reasoning, analysis, and deliberation on this decision space. A critical first step is to develop a model of the decision space, which will then allow for various methods and techniques to be applied in support of the larger architecture decision-making process. The Trade Space Ontology consists of a set of terminologies and relations (an ontology) and a MagicDraw resource that enables documentation of decisions and alternatives. It also provides a means by which decisions and alternatives can be traced to other Systems Engineering artifacts. For documenting alternatives, the Trade Space Ontology adapts the Morphological Matrix methodology to The Systems Modeling Language (SysML) through a profile; custom diagrams are also implemented to simplify the profile's use. With the profile and custom diagrams, system architects can specify options for architecture attributes, as well as compatibility between them, in a compact visual format. While the approach shares similarities to a trade tree, the emphasis at this stage is less on enumerating specific combinations of options and instead on specifying the options and their compatibility. Enumeration of alternatives is performed by an external analysis that operates on an output file from a model constructed using the Trade Space Ontology. For decisions, the Trade Space Ontology provides a way to model generic precedence relationships as well as documenting inputs and outputs. These may include what alternatives, criteria, and rationale are understood to be relevant for each decision. Importantly, the decision-making side of the Trade Space Ontology is defined at a more general level, such that it can be adapted to the specific terms in use by projects and programs at NASA. However, this adaptability also means that less capability is provided ``out-of-the-box'' from installation. Currently the resource includes plugin functionality to enumerate paths through generic precedence relationships between decisions and to export these paths to a spreadsheet. Custom dependency stereotypes are included in the profile to indicate the cross-cutting relationships between the trade space and the architecture decisions, providing a means to map which parts of the trade space enumerate alternatives for a decision, and to identify how the output of a decision may modify the trade space through pruning or down-selection. While the motivating use case for this resource is in human exploration architectures, the broad applicability of the Morphological Matrix methodology indicates that the Trade Space Ontology should also be useful for other activities and tasks at the agency.

Trade Tree↗

Anti-Fog Coatings For Spacesuit Helmets

A permanent and robust anti-fog coating was developed that is applied to the interior of polycarbonate spacesuit helmets. The coating is a mechanically resilient and permanent superhydrophilic (SHPL) layer produced by a two-step process consisting of a plasma processing pre-etch followed by the deposition of an optically transparent Diamond Like Carbon (DLC) coating to achieve an anti-fog, SHPL-DLC layer with Water Contact Angle (WCA) < 10°. The all-dry vacuum process consists of an oxygen plasma etching to engineer the surface of the polycarbonate, creating a well-controlled nanotextured morphology, which is key to the superhydrophilic properties, followed by vacuum deposited Plasma Enhanced Chemical Vapor Deposition (PECVD) DLC coating with high surface energy. The process is carried out at low temperatures compatible with polycarbonate materials and the two steps are performed back-to-back without breaking vacuum between steps. The process is readily scalable and compatible with large scale production volumes. The SHPL-DLC coating was tested for > 200 hours exposure using a breath simulator with no detrimental effect on the coating performance. The coating was also tested for exposure to cleaning fluids, IPA chemical exposure, adhesion testing per ASTM D3359, and abrasion resistance per MIL-E-12397 with no detrimental effects.

Spacesuit↗

The effects of temperature gradient and growth rate on the morphology and fatigue properties of MAR-M246(Hf)

MAR-M246(Hf) is a nickel based superalloy used in the turbopump blades of the Space Shuttle main engines. The effects are considered of temperature gradient (G) and growth rate (R) on the microstructure and fatigue properties of this superalloy. The primary dendrite arm spacings were found to be inversely proportional to both temperature gradient and growth rate. Carbide and gamma - gamma prime morphology trends were related to G/R ratios. Weibull analysis of fatigue results shows the characteristic life to be larger by a factor of 10 for the low gradient/fast rate pairing of G and R, while the reliability (beta) was lower.

Schmidt, D. D.↗

Morphology and Viability of Pleistocene Microbiota from the CRREL Permafrost Tunnel Near Fox, Alaska

The U. S. Army Cold Regions Research and Engineering Laboratory maintains the CRREL Permafrost Tunnel at Fox, Alaska (-10 miles north of Fairbanks.) The active microbial ecosystems and the cryopreserved anabiotic viable microorganisms and dead microbial remains and biomarkers frozen within the permafrost and ice of the CRREL Permafrost Tunnel are of direct relevance to Astrobiology. Microbial extremophiles from permafrost and ice provide information concerning where and how should we search for evidence of life elsewhere in the Cosmos. The permafrost and ice wedges of the Fox tunnel preserves a magnificent of record of Pliocene, Pleistocene and Holocene life on Earth spanning more than 2.5 million years. This record includes frozen fossil bacteria, archaea, algae, mosses, higher plants, insects and mammals. In this paper we present the preliminary results of studies of the morphology, ultramicrostructure and elemental distributions of Fox tunnel microbiota as determined in-situ by the Environmental Scanning Electron Microscope (ESEM) and the Field Emission Scanning Electron Microscope (FESEM) investigations. The long-term viability of cryopreserved microbiota and potential implications to Astrobiology will be discussed.

Hoover, Richard B.↗

Silicon production in a fluidized bed reactor

Part of the development effort of the JPL in-house technology involved in the Flat-Plate Solar Array (FSA) Project was the investigation of a low-cost process to produce semiconductor-grade silicon for terrestrial photovoltaic cell applications. The process selected was based on pyrolysis of silane in a fluidized-bed reactor (FBR). Following initial investigations involving 1- and 2-in. diameter reactors, a 6-in. diameter, engineering-scale FBR was constructed to establish reactor performance, mechanism of silicon deposition, product morphology, and product purity. The overall mass balance for all experiments indicates that more than 90% of the total silicon fed into the reactor is deposited on silicon seed particles and the remaining 10% becomes elutriated fines. Silicon production rates were demonstrated of 1.5 kg/h at 30% silane concentration and 3.5 kg/h at 80% silane concentration. The mechanism of silicon deposition is described by a six-path process: heterogeneous deposition, homogeneous decomposition, coalescence, coagulation, scavenging, and heterogeneous growth on fines. The bulk of the growth silicon layer appears to be made up of small diameter particles. This product morphology lends support to the concept of the scavenging of homogeneously nucleated silicon.

Rohatgi, N. K.↗

Multiple Concentric Cylinder Model (MCCM) user's guide

A user's guide for the computer program mccm.f is presented. The program is based on a recently developed solution methodology for the inelastic response of an arbitrarily layered, concentric cylinder assemblage under thermomechanical loading which is used to model the axisymmetric behavior of unidirectional metal matrix composites in the presence of various microstructural details. These details include the layered morphology of certain types of ceramic fibers, as well as multiple fiber/matrix interfacial layers recently proposed as a means of reducing fabrication-induced, and in-service, residual stress. The computer code allows efficient characterization and evaluation of new fibers and/or new coating systems on existing fibers with a minimum of effort, taking into account inelastic and temperature-dependent properties and different morphologies of the fiber and the interfacial region. It also facilitates efficient design of engineered interfaces for unidirectional metal matrix composites.

Williams, Todd O.↗

(abstract) A Low-Cost Mission to 2060 Chiron Based on the Pluto Fast Flyby

The Pluto Fast Flyby-based mission to Chiron described in this paper is a low cost, scientifically rewarding, focused mission in the outer solar system. The proposed mission will make a flyby of 2060 Chiron, an active 'comet' with over 10(sup 4) times the mass of Halley, and an eccentric, Saturn-crossing orbit which ranges from 8.5 to 19 AU. This mission concept achieves the flyby 4.2 years after launch on a direct trajectory from Earth, is independent of Jupiter launch windows, and fits within Discovery cost guidelines. This mission offers the scientific opportunity to examine a class of object left unsampled by the trail-blazing Mariners, Pioneers, Voyagers, and missions to Halley. Spacecraft reconnaissance of Chiron addresses unique objectives relating to cometary science, other small bodies, the structure of quasi-bound atmospheres on modest-sized bodies, and the origin of primitive bodies and the giant planets. Owing to Chiron's large size (180<D<370 km), unique nature, and unusual orbit, this mission is likely to draw significant public interest. As described by COMPLEX, the SSEC, and later the SSES, flybys are the appropriate scale missions for initial reconnaissance missions. Carrying three sophisticated instruments, the proposed flyby will return critical data about Chiron's size, shape, polar obliquity, atmosphere, surface morphology, surface composition, internal structure, surface activity (including the nature of Chiron's outbursts), and origin. Engineering analysis indicates that the spacecraft is capable of navigating to and encountering Chiron at close approach distances of less than 5 000 km, well inside the 50 000 to 150 000 km coma, and perhaps within the collisional chemistry zone of the coma. The low cost of the proposed Chiron mission is based on the opportunity to use the planned Pluto Flyby spare spacecraft and a Proton Expendable Launch Vehicle (ELV) (the pluto spacecraft is being designed to be compatible with a Proton launch). Backup launch opportunities on Delta II and Atlas ELVs are available. The Pluto Fast Flyby mission plans to develop a low cost ($150M), lightweight (<150 kg) outer planet spacecraft which is well suited to flyby reconnaissance in the outer solar system. This Chiron flyby mission is designed to leverage SSED's Pluto spacecraft investment into a scientifically valuable successor mission within the Discovery cost cap. Taking advantage of the spare spacecraft, we estimate the Discovery Chiron intercept mission can be launched for less than $100 M. We believe this makes a highly attractive proposal, which maintains US presence and leadership in the study of the outer solar system, and enables the first-time exploration of a wholly new class of planetary target, an outer solar system planetesimal.

2060 Chiron planetesimal flyby Pluto Fast Flyby sp↗

A Correlation Between the Intrinsic Brightness and Average Decay Rate of Gamma-Ray Burst X-Ray Afterglow Light Curves

We present a correlation between the average temporal decay (alpha X,avg, greater than 200 s) and early-time luminosity (LX,200 s) of X-ray afterglows of gamma-ray bursts as observed by the Swift X-ray Telescope. Both quantities are measured relative to a rest-frame time of 200 s after the gamma-ray trigger. The luminosity â€" average decay correlation does not depend on specific temporal behavior and contains one scale-independent quantity minimizing the role of selection effects. This is a complementary correlation to that discovered by Oates et al. in the optical light curves observed by the Swift Ultraviolet Optical Telescope. The correlation indicates that, on average, more luminous X-ray afterglows decay faster than less luminous ones, indicating some relative mechanism for energy dissipation. The X-ray and optical correlations are entirely consistent once corrections are applied and contamination is removed. We explore the possible biases introduced by different light-curve morphologies and observational selection effects, and how either geometrical effects or intrinsic properties of the central engine and jet could explain the observed correlation.

gamma-ray burst: general X-rays: burst↗

Challenges in Scaling Analysis from Coupons to Elements in 3D Woven Composites for a Launch Vehicle End-Ring Structure

The limitations of structural design with 3D woven composites based on homogenized material models characterized using coupon test data, representative unit cell analysis, or a combination of the two are discussed in the context of a space launch system (SLS)-scale end ring. Selected building block test configurations at the coupon, element, and subcomponent scales are described in detail. Highly instrumented tests and X-ray computed tomography inspections are used to characterize the structural behavior and associated damage progression with increasing load. It is shown that a single homogenized material constitutive relationship, applied at coupon and element scale, is not sufficient to model the structural response observed in both tests. In fact, substantially different stress-strain laws are required to predict the response of the coupon and element scale specimens, despite similar loading conditions. A model with discrete representation of the binder tows and cohesive interfaces to model matrix cracking shows much better agreement with the test data at the two length scales. Thus, evidence is provided showing that predictive structural analysis of 3D woven composites requires discrete representation of the tows, matrix cracking morphology, and their interactions in the structural configuration. With accurate knowledge of the element scale behavior, engineering approximations readily enable analysis scale-up to the subcomponent level.

Andrew C Bergan↗