Hydrogenase of coleman's sulfate-reducing bacterium.
Hydrogenase enzyme property study in order to elucidate process of sulfate reduction in Coleman organism
BACTERIOLOGY↗
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Effect of manganese deficiency on growth and chlorophyll content of algae with and without hydrogenase.
Manganese deficiency effect on growth and chlorophyll content of algae with and without hydrogenase
Kessler, E.↗
Light-dependent hydrogen production by C. reinhardi
The activity of hydrogenase in nonsynchronous, photoheterotrophically grown cells of C. reinhardi is a function of culture age. Rapidly growing cultures (exponential phase) exhibit lower hydrogenase activity than early stationary phase cultures. During prolonged dark anaerobic incubation the hydrogenase activity attains a maximal value in two to five hours. The activity declines rapidly after three to four hours of anaerobic incubation unless the pH of the suspending medium is maintained above 6.0. In C. reinhardi the source of electrons for hydrogen photoproduction appears to be derived mainly from water oxidation. However, when the water-splitting complex of photosystem II is impaired by a mutational block, the organism can utilize intracellular organic reductants as substrate for H2 production in a light-dependent reaction involving both PSII and PSI. When photosynthetic electron transport is uncoupled from phosphorylation, a rate of 174 micromoles of hydrogen evolved per mg cells per hour is observed. This rate of hydrogen photoproduction corresponds to 76% of the reductant generating capacity of PSII under steady-state photosynthesis.
Lien, S.↗
Changes of certain biochemical indices in animals during the effect of acceleration after gamma-irradiation
Tissue respiration and hydrogenase changes in gamma irradiated mice during acceleration
Abaturova, Ye. A.↗
The gas exchange of hydrogen-adapted algae as followed by mass spectrometry.
The combination of a mass spectrometer inlet and an oxygen electrode in the same vessel permitted continuous recording of the gas exchange of hydrogenase-containing anaerobically adapted algae. In contrast to conventional manometry, the present method made it possible to discern the simultaneous course of reactions involving O2, CO2, and H2. The experiments strongly support the idea of a balance between the photoproduction and photoutilization of H2.
Stuart, T. S.↗