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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2020

Assessment of holographic microscopy for quantifying marine particle size and concentration

Holographic microscopy has emerged as a tool for in situ imaging of microscopic organisms and other particles in the marine environment: appealing because of the relatively larger sampling volume and simpler optical configuration compared to other imaging systems. However, its quantitative capabilities have so far remained uncertain, in part because hologram reconstruction and image recognition have required manual operation. Here, we assess the quantitative skill of our automated hologram processing pipeline (CCV Pipeline), to evaluate the size and concentration measurements of environmental and cultured assemblages of marine plankton particles, and microspheres. Over 1 million particles, ranging from 10 to 200 μm in equivalent spherical diameter, imaged by the 4‐Deep HoloSea digital inline holographic microscope (DIHM) are analyzed. These measurements were collected in parallel with a FlowCam (FC), Imaging FlowCytobot (IFCB), and manual microscope identification. Once corrections for particle location and nonuniform illumination were developed and applied, the DIHM showed an underestimate in ESD of about 3% to 10%, but successfully reproduced the size spectral slope from environmental samples, and the size distribution of cultures (Dunaliella tertiolecta, Heterosigma akashiwo, and Prorocentrum micans) and microspheres. DIHM concentrations (order 1 to 1000 particles ml(exp −1)) showed a linear agreement (r(exp 2) = 0.73) with the other instruments, but individual comparisons at times had large uncertainty. Overall, we found the DIHM and the CCV Pipeline required extensive manual correction, but once corrected, provided concentration and size estimates comparable to the other imaging systems assessed in this study. Holographic cameras are mechanically simple, autonomous, can operate at very high pressures, and provide a larger sampling volume than comparable lens‐based tools. Thus, we anticipate that these characterization efforts will be rewarded with novel discovery in new oceanic environments.

Noah L. Walcutt↗
NASA NTRS2020

Assessment of Holographic Microscopy for Quantifying Marine Particle Size and Concentration

Holographic microscopy has emerged as a tool for in situ imaging of microscopic organisms and other particles in the marine environment: appealing because of relatively larger sampling volumes and simpler optical configurations compared to similar imaging systems. However, its quantitative capabilities have remained uncertain, in part because hologram reconstruction and image recognition have required manual operation. Here we assess the quantitative skill of our automated hologram processing pipeline, the CCV Pipeline, to evaluate the size and concentration measurements of environmental and cultured assemblages of marine plankton particles, and microspheres. Over one-million particles, ranging from 10-200 microns equivalent spherical diameter (ESD), imaged by the 4-Deep HoloSea digital inline holographic microscope (DIHM) are analyzed. These measurements were collected in parallel with FlowCam, Imaging FlowCytobot, and manual microscope identification. Once corrections for particle location and nonuniform illumination were developed and applied, the DIHM showed an underestimate in ESD of about 3-10%, but successfully reproduced the size-spectral-slope from environmental samples, and the size distribution of monocultures and microspheres. DIHM concentrations (order 1-1000 particles~ml-1) showed linear agreement (r^2=0.73) with the other instruments, but individual comparisons at times had large uncertainty.

holograpy↗
DOE OSTI2016

Recent progress in backreacted bottom-up holographic QCD

Recent progress in constructing holographic models for QCD is discussed, concentrating on the bottom-up models which implement holographically the renormalization group flow of QCD. The dynamics of gluons can be modeled by using a string-inspired model termed improved holographic QCD, and flavor can be added by introducing space filling branes in this model. The flavor fully backreacts to the glue in the Veneziano limit, giving rise to a class of models which are called V-QCD. The phase diagrams and spectra of V-QCD are in good agreement with results for QCD obtained by other methods.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
NASA NTRS1987

Temperature and concentration measurements in combustion

A high speed two wavelength holographic interferometer is being used to simultaneously measure temperature and concentration distributions for ignition and flame spread. Lines from a cw CO2 laser were used and the holograms recorded on a movie at 500 frames per/sec, individual frames were digitized with a microdensitometer. The detection on the fringe center was a major problem.

Kashiwagi, Takashi↗
NASA NTRS1990

Development of non-intrusive instrumentation for NASA-Ames Ballistic Range and Shock Tunnel

The use of nonintrusive instrumentation in high-enthalpy facilities offers the opportunity to collect data that has previously been difficult or impossible to obtain. Three such instruments, now under development, are described: an OH absorption system which can measure OH species concentration and temperature, a holographic interferometer for flow visualization and density measurement at low density, and a telemetry system which allows the measurement of surface quantities on model in free-flight at hypervelocity. The challenges and opportunities encountered when employing these advanced diagnostics to high-enthalpy facilities are discussed.

Strawa, A. W.↗
NASA NTRS1992

Study of FES/CAST/HGS

The microgravity materials processing program has been instrumental in providing the crystal growth community with an experimental environment to better understand the phenomena associated with the growing of crystals. In many applications one may pursue the growth of large single crystals which cannot be grown on earth due to convective driven flows. A microgravity environment is characterized by neither convection of buoyancy. Consequently superior crystals are able to be grown in space. On the other hand, since neither convection nor buoyancy dominates the fluid flow in a microgravity environment, then lesser dominating phenomena can affect crystal growth, such as surface driven flows or diffusion limited solidification. In the case of experiments that are to be flown in space using the Fluid Experiments System (FES), diffusion limited growth should be the dominating phenomenon. The use of holographic and Schlieren optical techniques for studying the concentration gradients in solidification processes has been used by several investigators over the years. The Holographic Ground System (HGS) facility at MSFC has been a primary resource in researching this capability. Consequently scientific personnel have been able to utilize these techniques in both ground based research and in space experiments. An important event in the scientific utilization of the HGS facilities was the TGS (triglycine sulfate) Crystal Growth and the Casting and Solidification Technology (CAST) experiments that were flown on the International Microgravity Lab (IML) mission in March of this year. The preparation and processing of these space observations are the primary experiments reported in this work. This project provides some ground-based studies to optimize on the holographic techniques used to acquire information about the crystal growth processes flown on IML. Since the ground-based studies will be compared with the space-based experimental results, it is necessary to conduct sufficient ground based studies to best determine how the experiment in space worked. The current capabilities in computer based systems for image processing and numerical computation have certainly assisted in those efforts. As anticipated, this study has certainly shown that these advanced computing capabilities are helpful in the data analysis of such experiments.

Workman, Gary L.↗
NASA NTRS1990

Two-color holography concept (T-CHI)

The Material Processing in the Space Program of NASA-MSFC was active in developing numerous optical techniques for the characterization of fluids in the vicinity of various materials during crystallization and/or solidification. Two-color holographic interferometry demonstrates that temperature and concentration separation in transparent (T-CHI) model systems is possible. The experiments were performed for particular (succinonitrile) systems. Several solutions are possible in Microgravity Sciences and Applications (MSA) experiments on future Shuttle missions. The theory of the T-CHI concept is evaluated. Although particular cases are used for explanations, the concepts developed will be universal. A breadboard system design is also presented for ultimate fabrication and testing of theoretical findings. New developments in holography involving optical fibers and diode lasers are also incorporated.

Vikram, C. S.↗
DOE OSTI2019

Simulation and partial prototyping of an eight‐junction holographic spectrum‐splitting photovoltaic module

Abstract Spectrum‐splitting photovoltaics incorporate optical elements to separate sunlight into frequency bands, which can be targeted at solar cells with bandgaps optimized for each sub‐band. Here, we present the design of a holographic diffraction grating‐based spectrum‐splitting photovoltaic module integrating eight III‐V compound semiconductor cells as four dual‐junction tandems. Four stacks of simple sinusoidal volume phase holographic diffraction gratings each simultaneously split and concentrate sunlight onto cells with bandgaps spanning the solar spectrum. The high‐efficiency cells get an additional performance boost from concentration incorporated using a single or a compound trough concentrator, providing up to 380X total concentration. Cell bandgap optimization incorporated an experimentally derived bandgap‐dependent external radiative efficiency function. Simulations show 33.2% module conversion efficiency is achievable. One grating stack is experimentally fabricated and characterized.

Darbe, Sunita↗
NASA NTRS1992

Study of Fluid Experiment System (FES)/CAST/Holographic Ground System (HGS)

The use of holographic and schlieren optical techniques for studying the concentration gradients in solidification processes has been used by several investigators over the years. The HGS facility at MSFC has been primary resource in researching this capability. Consequently, scientific personnel have been able to utilize these techniques in both ground based research and in space experiments. An important event in the scientific utilization of the HGS facilities was the TGS Crystal Growth and the casting and solidification technology (CAST) experiments that were flown on the International Microgravity Laboratory (IML) mission in March of this year. The preparation and processing of these space observations are the primary experiments reported in this work. This project provides some ground-based studies to optimize on the holographic techniques used to acquire information about the crystal growth processes flown on IML. Since the ground-based studies will be compared with the space-based experimental results, it is necessary to conduct sufficient ground based studies to best determine how the experiment worked in space. The current capabilities in computer based systems for image processing and numerical computation have certainly assisted in those efforts. As anticipated, this study has certainly shown that these advanced computing capabilities are helpful in the data analysis of such experiments.

Workman, Gary L.↗
NASA NTRS1995

Two color holographic interferometry for microgravity application

Holographic interferometry is a primary candidate for determining temperature and concentration in crystal growth experiments designed for space. The method measures refractive index changes within the fluid of an experimental test cell resulting from temperature and/or concentration changes. When the refractive index changes are caused by simultaneous temperature and concentration changes, the contributions of the two effects cannot be separated by single wavelength interferometry. By using two wavelengths, however, two independent interferograms can provide the additional independent equation required to determine the two unknowns. There is no other technique available that provides this type of information. The primary objectives of this effort were to experimentally verify the mathematical theory of two color holographic interferometry (TCHI) and to determine the practical value of this technique for space application. In the foregoing study, the theory of TCHI has been tested experimentally over a range of interest for materials processing in space where measurements of temperature and concentration in a solution are required. New techniques were developed and applied to stretch the limits beyond what could be done with existing procedures. The study resulted in the production of one of the most advanced, enhanced sensitivity holographic interferometers in existence. The interferometric measurements made at MSFC represent what is believed to be the most accurate holographic interferometric measurements made in a fluid to date. The tests have provided an understanding of the limitations of the technique in practical use.

Trolinger, James D.↗
NASA NTRS1993

Two color holographic interferometry for microgravity application

Holographic interferometry is a primary candidate for the measurement of temperature and concentration in various crystal growth experiments destined for space. The method measures refractive index changes in the experiment test cell. A refractive index change can be caused by concentration changes, temperature changes, or a combination of temperature and concentration changes. If the refractive index changes are caused by temperature and concentration changes occurring simultaneously in the experiment test cell, the contributions by the two effects cannot be separated by conventional measurement methods. By using two wavelengths, two independent interferograms can be produced from the reconstruction of the hologram. The two interferograms will be different due to dispersion properties of fluid materials. These differences provide the additional information that allows the separation of simultaneously occurring temperature and concentration gradients. There is no other technique available that can provide this type of information. The primary objectives of this effort are to experimentally verify the mathematical theory of two color holographic interferometry and to determine the practical value of this technique for space application. To achieve these objectives, the accuracy and sensitivity of the technique must be determined for geometry's and materials that are relevant to the Materials Processing in the Space program of NASA. This will be achieved through the use of a specially designed two-color holographic interferometry breadboard optical system. In addition to experiments to achieve the primary goals, the breadboard will also provide inputs to the design of an optimum space flight system.

Trolinger, James D.↗
NASA NTRS2005

Sol-Gel Glass Holographic Light-Shaping Diffusers

Holographic glass light-shaping diffusers (GLSDs) are optical components for use in special-purpose illumination systems (see figure). When properly positioned with respect to lamps and areas to be illuminated, holographic GLSDs efficiently channel light from the lamps onto specified areas with specified distributions of illumination for example, uniform or nearly uniform irradiance can be concentrated with intensity confined to a peak a few degrees wide about normal incidence, over a circular or elliptical area. Holographic light diffusers were developed during the 1990s. The development of the present holographic GLSDs extends the prior development to incorporate sol-gel optical glass. To fabricate a holographic GLSD, one records a hologram on a sol-gel silica film formulated specially for this purpose. The hologram is a quasi-random, micro-sculpted pattern of smoothly varying changes in the index of refraction of the glass. The structures in this pattern act as an array of numerous miniature lenses that refract light passing through the GLSD, such that the transmitted light beam exhibits a precisely tailored energy distribution. In comparison with other light diffusers, holographic GLSDs function with remarkably high efficiency: they typically transmit 90 percent or more of the incident lamp light onto the designated areas. In addition, they can withstand temperatures in excess of 1,000 C. These characteristics make holographic GLSDs attractive for use in diverse lighting applications that involve high temperatures and/or requirements for high transmission efficiency for ultraviolet, visible, and near-infrared light. Examples include projectors, automobile headlights, aircraft landing lights, high-power laser illuminators, and industrial and scientific illuminators.

Yu, Kevin↗
DOE OSTI2016

Opto-electrochemical spectroscopy of metals in aqueous solutions

In the present investigation, holographic interferometry was utilized for the first time to determine the rate change of the electrical resistance of aluminium samples during the initial stage of anodisation processes in aqueous solution. In fact, because the resistance values in this investigation were obtained by holographic interferometry, electromagnetic method rather than electronic method, the abrupt rate change of the resistance was called electrical resistance–emission spectroscopy. The anodisation process of the aluminium samples was carried out by electrochemical impedance spectroscopy (EIS) in different sulphuric acid concentrations (1.0%–2.5% H{sub 2}SO{sub 4}) at room temperature. In the meantime, the real time holographic interferometry was used to determine the difference between the electrical resistance of two subsequent values, dR, as a function of the elapsed time of the EIS experiment for the aluminium samples in 1.0%, 1.5%, 2.0%, and 2.5% H{sub 2}SO{sub 4} solutions. The electrical resistance–emission spectra of the present investigation represent a detailed picture of not only the rate change of the electrical resistance throughout the anodisation processes but also the spectra represent the rate change of the growth of the oxide films on the aluminium samples in different solutions. As a result, a new spectrometer was developed based on the combination of the holographic interferometry and electrochemical impedance spectroscopy for studying in situ the electrochemical behavior of metals in aqueous solutions.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
NASA NTRS1973

Sensitivity and fatigue of LiTaO3 for holographic recording

The sensitivity of crystals of LiTaO3 to hologram formation was observed to vary with impurity concentration from a value comparable to the most sensitive doped LiNbO3 for an impure crystal to a value more than five orders of magnitude smaller for a purer crystal. Fatigue effects were observed upon write-erase cycling. These effects were dependent upon writing and erasure polarization and power and could be virtually eliminated by proper choice of optical parameters.

Spinhirne, J. M.↗