Heterostructured materials: superior properties from hetero-zone interaction
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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
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Results for “forward stress”
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Design of a V–Ti–Ni alloy with superelastic nano-precipitates
Stress-induced martensitic transformations enable metastable alloys to exhibit enhanced strain hardening capacity, leading to improved formability and toughness. As is well-known from transformation-induced plasticity (TRIP) steels, however, the resulting martensite can limit ductility and fatigue life due to its intrinsic brittleness. In this work, we explore an alloy design strategy that utilizes stress-induced martensitic transformations but does not retain the martensite phase. This strategy is based on the introduction of superelastic nano-precipitates, which exhibit reverse transformation after initial stress-induced forward transformation. To this end, utilizing ab-initio simulations and thermodynamic calculations we designed and produced a V 45 Ti 30 Ni 25 (at%) alloy. In this alloy, TiNi is present as nano-precipitates uniformly distributed within a ductile V-rich base-centered cubic (bcc) β matrix, as well as being present as a larger matrix phase. We characterized the microstructure of the produced alloy using various scanning electron microscopy (SEM) and transmission electron microscopy (TEM) methods. Furthermore, the bulk mechanical properties of the alloy are demonstrated through tensile tests, and the reversible transformation in each of the TiNi morphologies were confirmed by in-situ TEM micro-pillar compression experiments, in-situ high-energy diffraction synchrotron cyclic tensile tests, indentation experiments, and differential scanning calorimetry experiments. The observed transformation pathways and variables impacting phase stability are critically discussed.
59 BASIC BIOLOGICAL SCIENCES↗
In-situ synchrotron X-ray diffraction investigation on deformation behavior of Nb/NiTi composite during pre-straining process
The mechanisms responsible for deformation behavior in Nb/NiTi composite during pre-straining were investigated systematically using in-situ synchrotron X-ray diffraction, transmission electron microscopy and tensile test. It is shown that upon loading, the composite experiences elastic elongation and slight plastic deformation of B19', B2 and β-Nb phases, together with the forward stress-induced martensitic (SIM) transformation from B2 to B19'. Upon unloading, the deformation mechanisms of the composite mainly involve elastic recovery of B19', B2 and β-Nb phases, compression deformation of β-Nb phase and incomplete B19'→B2 reverse SIM transformation. In the tensile loading-unloading procedure, besides the inherent elastic deformation and SIM transformation, the (001) compound twins in B19' martensite can also be conducive to the elastic deformation occurring in B19'-phase of the composite. Therefore, this composite can exhibit a large recoverable strain after unloading owing to the elastic deformation, and the partially reversible and consecutive SIM transformation together with the (001) compound twins.
36 MATERIALS SCIENCE↗
Activation of the plant mevalonate pathway by extracellular ATP
The mevalonate pathway plays a critical role in multiple cellular processes in both animals and plants. In plants, the products of this pathway impact growth and development, as well as the response to environmental stress. A forward genetic screen of Arabidopsis thaliana using Ca 2+ -imaging identified mevalonate kinase (MVK) as a critical component of plant purinergic signaling. MVK interacts directly with the plant extracellular ATP (eATP) receptor P2K1 and is phosphorylated by P2K1 in response to eATP. Mutation of P2K1-mediated phosphorylation sites in MVK eliminates the ATP-induced cytoplasmic calcium response, MVK enzymatic activity, and suppresses pathogen defense. The data demonstrate that the plasma membrane associated P2K1 directly impacts plant cellular metabolism by phosphorylation of MVK, a key enzyme in the mevalonate pathway. The results underline the importance of purinergic signaling in plants and the ability of eATP to influence the activity of a key metabolite pathway with global effects on plant metabolism.
59 BASIC BIOLOGICAL SCIENCES↗
A Comparison of Ion Implantation at Room Temperature and Heated Ion Implantation on the Body Diode Degradation of Commercial 3.3 kV 4H-SiC Power MOSFETs
It has been demonstrated that basal plane dislocations (BPDs)-induced stacking faults (SFs) cause body diode degradation in commercial 4H-SiC power MOSFETs, especially with higher voltage ratings. BPDs originate from 4H-SiC boule, epi growth, and ion implantation. Considering the lower cost of ion implantation at room temperature (RT), this work investigates the potential of RT ion implantation replacing heated (HT) ion implantation by comparing the influence of both ion implantations on the body diode degradation of commercial 3.3 kV 4H-SiC power MOSFETs. We demonstrate with long-term (up to 1000 hours) forward current stress that RT implantation can keep the body diode degradation of 3.3 kV 4H-SiC power MOSFETs within the specification limits compared with HT implantation.
4H-SiC↗
Harnessing Heterologous Bacterial Two-Component Systems as Biosensors to Address Challenges in Fermentation Scale-Up
Scaling up bacterial fermentation from bench to industrial scale often results in unpredictable performance losses, possibly in part due to changes in microenvironmental conditions such as pH. To investigate this, we developed a suite of pH-sensitive biosensors from bacterial two-component systems (TCSs) that provide a dynamic, fluorescent readout in response to extracellular pH changes. TCSs consist of a periplasmic sensor histidine kinase (HK) that, in response to an extracellular stimulus, autophosphorylates intracellularly and subsequently transfers the phosphate to a cognate response regulator (RR) that modulates transcription of target genes. We utilized three pH-responsive TCSs (referred to here as CVJ1, CVJ30, and CVJ79) and linked their output to GFP. This was achieved by placing the RR promoter upstream of GFP or by constructing a chimeric RR composed of the native receiver domain and the DNA-binding domain of another well-characterized RR with a defined promoter. All components - HK, RR (native or chimeric), and GFP under its corresponding promoter - were cloned into a broad-host-range plasmid. Sensors were validated in Escherichia coli and Pseudomonas putida, including the muconic acid-producing strain P. putida TL207. All three biosensors successfully reported pH, with fluorescence (normalized to optical density) correlating strongly with media pH. Among the native sensors, CVJ79 showed the most robust performance while CVJ1 also performed best in its native form; CVJ30 exhibited improved functionality as a chimera, suggesting that modular RR design can enhance compatibility in some heterologous hosts. Further, CVJ79 was activated by alkaline conditions, while CVJ30 responded to acidic environments. Notably, CVJ1 was induced by high pH in wild-type E. coli and P. putida, but low pH in TL207. The observed differences in sensor activation between strains - particularly the divergent response of CVJ1 - suggest that host-specific regulatory pathways may influence how cells perceive and adapt to pH stress. Moving forward, these biosensors can be used to guide the rational design of more robust strains, optimize process conditions in real time, and inform strategies to minimize physiological heterogeneity during scale-up. Integrating these tools into high-throughput screening and bioreactors will be a key step toward improving predictability and performance in industrial bioprocesses.
09 BIOMASS FUELS↗
A Dominant Arabidopsis Thaliana ACTIN7 Mutant for Studies of Cytoskeletal-Mediated Root Gravity and Spaceflight Stress Responses
A forward genetic screen for Arabidopsis thaliana mutants that exhibited differential sensitivity to the actin-disrupting compound, latrunculin B (LatB), was conducted to uncover new players involved in actin-mediated root gravity responses. This to the isolation of a mutant that exhibited robust primary root growth at 100 nM LatB, which is a concentration that severely inhibits wild-type root elongation. Phenotypic analysis revealed that hypocotyl elongation in the dark and root hair tip growth in the mutant could tolerate LatB concentrations that impaired these processes in wild type. A cross between the mutant and wild type resulted in progeny resistance to LatB, which indicated that the mutant is dominant (hereafter referred to as LBR1 for LatB Resistant1). Filamentous-actin (F-actin) organization in LBR1 primary roots remained intact at 100 nM LatB, while that of wild type exhibited fragmented F-actin. Next generation sequencing revealed that LBR1 had a single nucleotide polymorphism (SNP) in the AT5G09810 gene that changed cytosine to a thiamine at the first exon. AT5G09810 encodes ACTIN7 (ACT7), which is one of three vegetative actin isoforms in A. thaliana. The SNP in the ACT7 gene led to a change in a single amino acid from proline at position 32 to a serine. Transgenic complementation of LBR1 plants with wild-type ACT7 under the control of the ACT7 promoter (pACT7:ACT7) and wild-type plants with LBR1, which contained the proline to serine mutation, also under the ACT7 promoter (pACT7:LBR1) confirmed that LBR1 is ACT7. The pACT7:LBR1 construct was also able to confer LatB resistance to the act7-5 and act2-3 vegetative ACT mutants. Moreover, LBR1 exhibited partial tolerance to salt and low phosphate, and enhanced root skewing on a clinostat, suggesting that site-directed engineering of vegetative ACT presents a strategy for generating stress-tolerant plants for spaceflight applications and studies of actin-mediated gravity responses.
Plant Space Biology↗
A Dominant Arabidopsis Thaliana ACTIN7 Mutant for Studies of Cytoskeletal-Mediated Root Gravity and Spaceflight Stress Responses
A forward genetic screen for Arabidopsis thaliana mutants that exhibited differential sensitivity to the actin-disrupting compound, latrunculin B (LatB), was conducted to uncover new players involved in actin-mediated root gravity responses. This led to the isolation of a mutant that exhibited robust primary root growth at 100 nM LatB, which is a concentration that severely inhibits wild-type root elongation. Phenotypic analysis revealed that hypocotyl elongation in the dark and root hair tip growth in the mutant could tolerate LatB concentrations that impaired these processes in wild type. A cross between the mutant and wild type resulted in progeny resistance to LatB, which indicated that the mutant is dominant (hereafter referred to as LBR1 for LatB Resistant1). Filamentous-actin (F-actin) organization in LBR1 primary roots remained intact at 100 nM LatB, while that of wild type exhibited fragmented F-actin. Next generation sequencing revealed that LBR1 had a single nucleotide polymorphism (SNP) in the AT5G09810 gene that changed cytosine to a thiamine at the first exon. AT5G09810 encodes ACTIN7 (ACT7), which is one of three vegetative actin isoforms in A. thaliana. The SNP in the ACT7 gene led to a change in a single amino acid from proline at position 32 to a serine. Transgenic complementation of LBR1 plants with wild-type ACT7 under the control of the ACT7 promoter (pACT7:ACT7) and wild-type plants with LBR1, which contained the proline to serine mutation, also under the ACT7 promoter (pACT7:LBR1) confirmed that LBR1 is ACT7. The pACT7:LBR1 construct was also able to confer LatB resistance to the act7-5 and act2-3 vegetative ACT mutants. Moreover, LBR1 exhibited partial tolerance to salt and low phosphate, and enhanced root skewing on a clinostat, suggesting that site-directed engineering of vegetative ACT presents a strategy for generating stress-tolerant plants for spaceflight applications and studies of actin-mediated gravity responses.
Plant Space Biology↗
Stress Studies at Kennedy Space Center: a Backward and Forward Look
Possible relationships between occupational and other stresses on ischemic heart disease are explored. Three procedures were used: (1) double master 2-step test, (2) dynamic ECG technique using avionics equipment, and (3) submaximal stress testing with Marco bicycle ergometer.
A. I. Decker↗
A Description of the Design of Highly Swept Propeller Blades
A description of the two swept propellers investigated in the Langley 8-foot high-speed tunnel is presented, together with the discussions of the numerous assumptions and analyses on which the designs of these propellers are based. The blades are swept considerably along the entire blade radius and, in order to allow for reductions in the maximum stresses, are swept forward inboard and backward outboard. The blades have been designed on the basis of the blade-element method primarily to have subcritical efficiencies at the highest possible forward speed. The designs have been controlled primarily by the stresses in the blades. (author)
Whitcomb, Richard T↗
Research on gravitational mass sensors Research contract status report, 15 Sep. - 15 Oct. 1965
Gravitational mass sensor - piezoresistive and barium titanate transducers, and torsional stress sensors
GRAVITY↗
Twin nucleation and growth in hexagonal close-packed metals: The role of slip-mediated plasticity on twin embryo formation and evolution
Twinning is a key deformation mechanism in hexagonal close-packed (hcp) metals, which are typified by a lack of easily activated slip systems that can accommodate a general state of loading. Pragmatically, the nucleation and evolution of twin domains occurs concomitantly with slip, such that the eventual twin network is conditioned by both external and internal stresses resulting, among others, from the evolution of dislocations. However our understanding of the interplay between dislocations, and twin nucleation and stability remains limited. This work focuses on elucidating the influence of dislocation-mediated plasticity on the formation, growth, and stability of twin embryos. First, a new mesoscale spectral crystal plasticity-twinning framework is developed and used to quantify the change in the free energy landscape following the nucleation {$10\bar{1}2$} of twins in Mg. Representative twin morphologies are modeled under conditions of limited and profuse slip activity, which are emulative of small- and bulk-scale samples, respectively. Then, the driving traction profiles around twin embryos are investigated via a sharp interface approach to obtain insights into how concurrent slip-mediated plasticity can influence the growth/stabilization of nanometric twins. The driving traction profiles are further utilized to determine the stability of twin embryos post loading. The initial dislocation density in the samples, and within the different domains (i.e., twin vs. parent), is seen to have a significant effect on the twin nucleation stress. Namely, the activation of high levels of concomitant plasticity in the parent grain is seen to significantly drive the formation of nanometric twin nuclei at stresses as low as 250 MPa. Further, the sharp interface analysis reveals that profuse plasticity in the parent grain simultaneously alters the forward and back stresses, such that the magnitude/polarity of the driving tractions become increasingly favorable for nanometric twin growth when slip is active. Finally, prior plasticity in the parent grain is seen to result in favorable driving tractions for nanometric twin growth, even at applied stresses as low as ~ 100 MPa. In conclusion, these results are in stark contrast to a case without any dislocations, wherein applied stresses as high as ~ 500 MPa are necessary to grow the twin domains.
36 MATERIALS SCIENCE↗
Flight Measurements of the Vibratory Bending and Torsional Stresses on a Modified Supersonic Propeller for Forward Mach Numbers up to 0.95
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O'Bryan, Thomas C↗
Computation of turbulent flows over backward and forward-facing steps using a near-wall Reynolds stress model
Separation and reattachment of turbulent shear layers is observed in many important engineering applications, yet it is poorly understood. This has motivated many studies on understanding and predicting the processes of separation and reattachment of turbulent shear layers. Both of the situations in which separation is induced by adverse pressure gradient, or by discontinuities of geometry, have attracted attention of turbulence model developers. Formulation of turbulence closure models to describe the essential features of separated turbulent flows accurately is still a formidable task. Computations of separated flows associated with sharp-edged bluff bodies are described. For the past two decades, the backward-facing step flow, the simplest separated flow, has been a popular test case for turbulence models. Detailed studies on the performance of many turbulence models, including two equation turbulence models and Reynolds stress models, for flows over steps can be found in the papers by Thangam & Speziale and Lasher & Taulbee). These studies indicate that almost all the existing turbulence models fail to accurately predict many important features of back step flow such as reattachment length, recovery rate of the redeveloping boundary layers downstream of the reattachment point, streamlines near the reattachment point, and the skin friction coefficient. The main objectives are to calculate flows over backward and forward-facing steps using the NRSM and to make use of the newest DNS data for detailed comparison. This will give insights for possible improvements of the turbulence model.
Ko, Sung HO↗
Endochronic theory of transient creep and creep recovery
Short time creep and creep recovery were investigated by means of the endochronic theory of viscoplasticity. It is shown that the constitutive equations for constant-strain-rate stress-strain behavior, creep, creep recovery, and stress relaxation can all ber derived from the general constitutive equation by imposing appropriate constraints. In this unified approach, the effect of strain-hardening is naturally accounted for when describing creep and creep recovery. The theory predicts with reasonable accuracy the creep and creep recovery behaviors for Aluminum 1100-0 at 150 C. It was found that the strain-rate history at prestraining stage affects the subsequent creep. A critical stress was also established for creep recovery. The theory predicts a forward creep for creep recovery stress greater than the critical stress. For creep recovery stress less than the critical stress, the theory then predicts a normal strain recovery.
Wu, H. C.↗
Contact integrity testing of stress-tested silicon terrestrial solar cells
A test procedure was developed and applied to terrestrial silicon solar cells in order to determine the effect of accelerated environmental and time-temperature aging on metal contact integrity. Quantities of cells of four different manufacturers were given the contact integrity test after being subjected to accelerated stress tests that included forward bias-temperature, thermal cycle and thermal shock, power cycle, and bias-temperature humidity tests at two temperature-humidity levels. Significant effects due to certain stress tests were found for some cell types. It is concluded that cells fabricated using plated nickel/solder metallization showed significantly more serious contact integrity degradation than silver-metallized cells.
Prince, J. L.↗
Temperature effect on stress concentration around circular hole in a composite material specimen representative of X-29A forward-swept wing aircraft
The theory of anisotropic elasticity was used to evaluate the anisotropic stress concentration factors of a composite laminated plate containing a small circular hole. This advanced composite was used to manufacture the X-29A forward-swept wing. It was found for composite material, that the anisotropic stress concentration is no longer a constant, and that the locations of maximum tangential stress points could shift by changing the fiber orientation with respect to the loading axis. The analysis showed that through the lamination process, the stress concentration factor could be reduced drastically, and therefore the structural performance could be improved. Both the mixture rule approach and the constant strain approach were used to calculate the stress concentration factor of room temperature. The results predicted by the mixture rule approach were about twenty percent deviate from the experimental data. However, the results predicted by the constant strain approach matched the testing data very well. This showed the importance of the inplane shear effect on the evaluation of the stress concentration factor for the X-29A composite plate.
Yeh, Hsien-Yang↗
Stress concentration around circular hole in a composite material specimen representative of the X-29A forward-swept wing aircraft
The theory of anisotropic elasticity was used to evaluate the anisotropic stress concentration factors of a composite laminated plate containing a small circular hole. This advanced composite material was used to manufacture the X-29A forward swept wing. Observe that the usual isotropic material stress concentration factor is three. However, for composite material, it was found that the anisotropic stress concentration factor is no longer constant, and that the locations of maximum tangential stress points could shift by changing the fiber orientation with respect to the loading axis. The analysis showed that through the lamination process, the stress concentration factor could be drastically reduced, and therefore the structural performance could be improved. Both the mixture rule approach and the constant strain approach were used to calculate the stress concentration factor. The results predicted by the mixture rule approach were about 20 percent deviate from the experimental data. However, the results predicted by the constant strain approach matched the testing data very well. This showed the importance of the inplane shear effect on the evaluation of stress concentration factor for the X-29A composite plate.
Yeh, Hsien-Yang↗