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At least 19 records

Rapid and highly fieldable viral diagnostic

The present invention relates to a rapid, highly fieldable, nearly reagentless diagnostic to identify active RNA viral replication in a live, infected cells, and more particularly in leukocytes and tissue samples (including biopsies and nasal swabs) using an array of a plurality of vertically-aligned nanostructures that impale the cells and introduce a DNA reporter construct that is expressed and amplified in the presence of active viral replication.

59 BASIC BIOLOGICAL SCIENCES↗

Fieldable Platform for Large-Scale Deposition of Concrete Structures

Oak Ridge National Laboratory’s Manufacturing Demonstration Facility is developing a novel, large-scale additive manufacturing, or 3D printing, system. The Sky Big Area Additive Manufacturing (SkyBAAM) system will ultimately be a fieldable concrete deposition machine with pick and place abilities that will allow for full-scale, automated construction of buildings. The system will be implemented with existing construction equipment meaning conventional cranes will be used to suspend the printhead. SkyBAAM will be cable-driven by four base stations and suspended from a single crane. The elimination of a gantry system, found commonly in large-scale additive manufacturing systems, will enable SkyBAAM to be quickly set up with minimal site preparation. The medium-scale version of SkyBAAM is currently in development. The system design, cable stiffness analysis, and tactics for freezing rotational degrees-of-freedom (DOF), detailed in this paper, will provide a basis for the final, large-scale version of the SkyBAAM system.

Chesser, Phillip↗

Design of Fieldable Head Space Manifold

A system has been designed and built to meet the expected requirements for sampling large UF 6 cylinders in the field and to provide similar purification and analyses to those currently performed for 1S and 2S samples at Oak Ridge National Laboratory (ORNL). The system was designed to be modular, allowing flexibility to perform under a variety of conditions. At a minimum, a large cylinder can be accessed to collect head space samples. These samples can additionally be processed in the field to generate 10 cc purified samples that should be similar to ones currently generated at ORNL from sampling of smaller cylinders. Variable inlet mass spectrometer operation can be performed at the time of purification or at a different location on a purified sample. This design should allow collection, processing and analysis of one extracted sample per day based on similar operations using the ORNL manifold. Reduced operations can be performed at a slightly faster rate although movement between cylinders does require time to evacuate and dry the connection adequately for accessing the UF 6 head space.

42 ENGINEERING↗

SkyBAAM Large-Scale Fieldable Deposition Platform System Architecture

Oak Ridge National Laboratory (ORNL) is currently developing a concrete deposition system for infrastructure-scale printed objects. This system, called SkyBAAM, uses a cable driven motion platform to manipulate the print head. This work focuses on the general aspects of the system architecture, including arrangement of the cable driven platform, general high-level control methodology, and system accuracy, along with concrete deposition methods. Results and demonstration prints will be shown.

Chesser, Phillip↗

Functionalized Polymer Thin Films for Plutonium Capture and Isotopic Screening from Aqueous Sources

The rapid screening of plutonium from aqueous sources remains a critical challenge for nuclear nonproliferation efforts. The determination of trace-level Pu isotopes in water requires offsite sample preparation and analysis; therefore, new methods that combine plutonium purification, concentration, and isotopic screening in a fieldable detection system will provide an invaluable tool for nuclear safeguards. This contribution describes the development and characterization of thin polymer-ligand films for the isolation and concentration of waterborne Pu for direct spectroscopic analyses. Submicron thin films were prepared through spin coating onto Si wafers and consisted of combinations of polystyrene (PS) with dibenzoylmethane, thenoyltrifluoroacetone, and di(2-ethylhexyl)phosphoric acid (HDEHP). Pu uptake studies from solutions at pH from 2.3 to 6.3 indicated that only films containing HDEHP exhibited significant recovery of Pu. High alpha spectroscopy peak energy resolutions were achieved for PS-HDEHP films over a range of film thicknesses from 30 to 250 nm. Furthermore, a separate study was performed to evaluate uptake from a primarily Pu(V) solution where it was observed that doubling the HDEHP loading in the film increased uptake of Pu by an order of magnitude. X-ray photoelectron spectroscopy (XPS) analysis revealed that HDEHP was highly concentrated within the first few nanometers of the film at the higher loading. XPS analysis also revealed that, in the presence of water, HDEHP was stripped from the surface layer of the film at circumneutral pH. While significant losses of ligand were seen in all samples, higher loadings of HDEHP resulted in measurable amounts of ligand retained after a 12-h soak in water. Findings of this study are being used to guide the development of thin-film composite membrane-based detection methods for the rapid, fieldable analysis of Pu in water.

36 MATERIALS SCIENCE↗

Isotope-resolved atomic beam laser spectroscopy of natural uranium

Here, we present our results on the development of a technique for conducting laser absorption spectroscopy of uranium in a collimated atomic beam using a compact and potentially fieldable device. A solid natural uranium metal sample is vaporized in a modest vacuum of several mTorr via resistive heating of a tantalum foil micro-crucible. A temperature of over 2000 °C is reached by applying only 200 W of electrical power to the crucible. At this temperature, the vapor pressure of uranium is large enough to generate a stable atomic beam. A diode laser is tuned around the 861.031 nm uranium transition, which allows the observation of absorption bands from both the 238 U and 235 U isotopes. The isotopic composition of uranium Standard Reference Material 960 is obtained from the absorption signal, in excellent agreement with the nominal value. In conclusion, our approach can be readily applied to the isotopic analysis of other actinides, and it is expected to lead to the development of compact, fieldable instrumentation.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

A decay database of coincident γ–γ and γ–X -ray branching ratios for in-field spectroscopy applications

Current fieldable spectroscopy techniques often use single detector systems heavily impacted by interferences from intense background radiation fields. These effects result in low-confidence measurements that can lead to misinterpretation of the collected spectrum. To help improve interpretation of the fission products and short-lived radionuclides produced in a composite sample, a coincidence-database is being developed in support of a robust portable and X-ray coincidence detector system concurrently under development at the Pacific Northwest National Laboratory for in-field deployment. Hitherto, no database exists containing coincident γ–γ and γ–X-ray branching-ratio intensities on an absolute scale that will greatly enhance isotopic identification for in-field applications. As part of this project, software has been developed to parse all radioactive-decay data sets from the Evaluated Nuclear Structure Data File (ENSDF) archive to enable translation into a more useful JavaScript Object Notation (JSON) formats that more readily supports query-based data manipulation. The coincident database described in this work is the first of its kind and contains coincidence γ–γ and γ–X-ray intensities and their corresponding uncertainties, together with auxiliary metadata associated with each decay data set. The new JSON format provides a convenient and portable means of data storage that can be imported into analysis frameworks with relatively low overhead allowing for meaningful comparison with measured data.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Note: Micro-channel array crucible for isotope-resolved laser spectroscopy of high-temperature atomic beams

Here, we present a novel compact design for a multichannel atomic oven which generates collimated beams of refractory atoms for fieldable laser spectroscopy. Using this resistively heated crucible, we demonstrate spectroscopy of an erbium sample at 1300 °C with improved isotopic resolution with respect to a single-channel design. In addition, our oven has a high thermal efficiency. By minimizing the surface area of the crucible, we achieve 2000 °C at 140 W of applied electrical power. As a result, the design does not require any active cooling and is compact enough to allow for its incorporation into fieldable instruments.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Novel Compact Ceilometer (Final Scientific-Technical Report)

Fibertek is developing a novel ceilometer system that provides a new measurement capability not currently available to weather stations such as those in the Department of Energy's AmeriFlux network. Commercially available instruments do not have the sensitivity to see atmospheric aerosols. If the atmospheric aerosols can be measured, then the altitude of the boundary layer can be determined from a discontinuity in the aerosol concentration. The altitude of the boundary layer is a critical parameter in climate and weather models, and adding this piece of information will greatly improve the accuracy of these models. Fibertek's design provides this capability in an inexpensive robust fieldable instrument by applying a new sensor technology: the Silicon Photomultiplier (SiPM). SiPMs are called "photomultipliers" in that they provide very similar performance as a traditional photomultiplier including single photon sensitivity and linear response. However, they are a completely different technology which offers several advantages. They are small, inexpensive, solid-state, and extremely robust. They do not require cooling. They operate at much lower voltages. They cannot be damaged by inadvertent reflections, and when saturated they recover in less than a microsecond, so they don't need to be shielded from the outgoing laser pulse. In short, they make single photon sensitivity accessible for an inexpensive, simple, rugged, fieldable system. In this Phase 1 effort Fibertek developed a breadboard version of the ceilometer to demonstrate the feasibility of our approach. Our partner, Penn State, conducted atmospheric measurements to validate the lidar’s performance and determine algorithms for automatically identifying the height of the boundary layer. The lidar shows sufficient sensitivity to fully characterize the boundary layer in clear air daytime and nighttime conditions.

47 OTHER INSTRUMENTATION↗

NMIS/FNMIS Comparison for US DOE Office of Nuclear Verification

A comparison of the characteristics of the Nuclear Materials Identification System (NMIS) and its more fieldable version that was developed for future treaty applications, the Fieldable Nuclear Materials Identification System (FNMIS), is presented. Both systems were developed for the US Department of Energy (DOE) Office of Nuclear Verification (ONV). Under the Warhead Safety and Security Exchange Agreement, NMIS was also jointly developed with the Russian Federation. The configuration of the systems and their characteristics are described. In addition, the capabilities, advantages and disadvantages, and a comparison fast-neutron transmission imaging measurement is presented. The comparison measurements gave essentially the same results because the software and imaging apparatus have the same capabilities, with the FNMIS completing the measurements slightly faster. NMIS is an open system that is more easily modified for research applications and is not suitable for the ONV mission, and FNMIS is compatible with an information barrier, which is more useful for ONV applications.

98 NUCLEAR DISARMAMENT, SAFEGUARDS, AND PHYSICAL P↗