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At least 19 records

Size and metabolic properties of single muscle fibers in rat soleus after hindlimb suspension

The effect of 28-day-long hind-limb suspension (HS) combined with 10 daily forceful lengthening contractions of the limb on the morphological and metabolic properties of individual fibers of the soleus was studied in rats, using quantitative histochemical techniques. Compared with nonsuspended controls (CON), soleus wet weights of HS rats were decreased by 49 percent; the fibers staining lightly for myosin ATPase ('light-ATPase' fibers) atrophied more than the 'dark-ATPase' fibers. Single-fiber alpha-glycerophosphate dehydrogenase (GPD) and succinate dehydrogenase (SDH) activities were higher in HS than in CON rats. Daily forceful lengthening contractions did not prevent the HS-induced changes. The results support the view that the soleus fibers can change from a slow-twitch oxidative to a fast-twitch oxidative-glycolytic profile, but rarely to a fast-twitch glycolytic one, and that the SDH and GPD activities per volume of tissue can be increased even when there are severe losses of contractile proteins.

Hauschka, Edward O.↗

Exercise effects on the size and metabolic properties of soleus fibers in hindlimb-suspended rats

The effects of four-week-long hind-limb suspension (HS) of rats on the size the soleus muscle fibers and the activity of succinate dehydrogenase (SDH) in dark and light ATPase fibers were investigated together with the efficacy of an endurance exercise (EX) program (daily treadmill exercise for 1.5 h/day at 20 m/min and a 30-percent grade) in ameliorating HS-induced changes. It was found that, in comparison to age-matched controls, the soleus wet weight decreased by 69 and 30 percent in HS and HS-EX rats, respectively, and the percent of dark ATPase fibers increased from 10 percent in controls to 19 and 17 percent, respectively. The values of the integrated fiber activity (activity/min times muscle area) showed a net loss of SDH in both the light and dark ATPase fibers of HS rats, but only in the light ATPase fibers of the HS-EX rats, indicating that exercise ameliorated but did not prevent the muscle fiber atrophy induced by HS.

Graham, Scot C.↗

Metabolic and morphologic properties of single muscle fibers in the rat after spaceflight, Cosmos 1887

The adaptation of a slow (soleus, Sol) and a fast (medial gastrocnemius, MG) skeletal muscle to spaceflight was studied in five young male rats. The flight period was 12.5 days and the rats were killed approximately 48 h after returning to 1 g. Five other rats that were housed in cages similar to those used by the flight rats were maintained at 1 g for the same period of time to serve as ground-based controls. Fibers were classified as dark or light staining for myosin adenosine triphosphatase (ATPase). On the average, the fibers in the Sol of the flight rats atrophied twice as much as those in the MG. Further, the fibers located in the deep (close to the bone and having the highest percentage of light ATPase and high oxidative fibers in the muscle cross section) region of the MG atrophied more than the fibers located in the superficial (away from the bone and having the lowest percentage of light ATPase and high oxidative fibers in the muscle cross-section) region of the muscle. Based on quantitative histochemical assays of single muscle fibers, succinate dehydrogenase (SDH) activity per unit volume was unchanged in fibers of the Sol and MG. However, in the Sol, but not the MG, the total amount of SDH activity in a 10-microns-thick section of a fiber decreased significantly in response to spaceflight. Based on population distributions, it appears that the alpha-glycerophosphate dehydrogenase (GPD) activities were elevated in the dark ATPase fibers in the Sol, whereas the light fibers in the Sol and both fiber types in the MG did not appear to change. The ratio of GPD to SDH activities increased in the dark (but not light) fibers of the Sol and was unaffected in the MG. Immunohistochemical analyses indicate that approximately 40% of the fibers in the Sol of flight rats expressed a fast myosin heavy chain compared with 22% in control rats. Further, 31% of the fibers in the Sol of flight rats expressed both fast and slow myosin heavy chains compared with 8% in control rats. Immunohistochemical changes in the MG were minimal. These data suggest that the magnitude and direction of enzymatic activity and cell volume changes are dependent on the muscle, the region of the muscle, and the type of myosin expressed in the fibers. Further, the ability of fibers to maintain normal or even elevated activities per unit volume of some metabolic enzymes is remarkable considering the marked and rapid decrease in fiber volume.

NASA Experiment Number COS 1887-26↗

Effects of periodic weight support on medial gastrocnemius fibers of suspended rats

The effects of seven-day-long hindlimb suspension (HS) and HS plus daily periodic weight support activity on the size and metabolic properties of individual fibers in the medial gastrocnemius (MG) of rats were examined. Sections of muscle tissue removed after seven day suspension were stained quantitatively for succinate dehydrogenase and alpha-glycerophosphate dehydrogenase, and qualitatively for myosin ATPase. It was found that short intermittent periods of weight support had a beneficial effect in maintaining the size and metabolic properties of both dark and light ATPase fibers in the deep regions (i.e., close to the bone) and of dark ATPase fibers in the superficial regions of the MG. The effect was greater in the deep regions.

Graham, Scot C.↗

Size and metabolic properties of fibers in rat fast-twitch muscles after hindlimb suspension

The effect of hind-limb suspension (HS) on single fibers of the medial gastrocnemius (MG) and the tibialis anterior (TA) muscles were studied in rats. Fiber area and the activities of succinate dehydrogenase (SDH) and alpha-glycerophosphate dehydrogenase (GPD) were determined in tissue sections using an image analysis system. After 28 days of HS, the MG atrophied 28 percent, whereas the TA weight was maintained. Both dark- and light-ATPase fibers in the deep region of the MG had decreased cross-sectional areas following HS, with the atrophic response being twice as great in the light-ATPase fibers than in the dark-ATPase fibers. Following HS, mean SDH activities of both fiber types were significantly lower in the MG and TA than in the CON; by contrast, mean GPD activities were either maintained at the CON level or were higher in both MG and TA muscles. The data suggest an independence of the mechanisms determining the muscle fiber size and the metabolic adaptations associated with HS.

Roy, Roland R.↗

Multifunction Imaging and Spectroscopic Instrument

A proposed optoelectronic instrument would perform several different spectroscopic and imaging functions that, heretofore, have been performed by separate instruments. The functions would be reflectance, fluorescence, and Raman spectroscopies; variable-color confocal imaging at two different resolutions; and wide-field color imaging. The instrument was conceived for use in examination of minerals on remote planets. It could also be used on Earth to characterize material specimens. The conceptual design of the instrument emphasizes compactness and economy, to be achieved largely through sharing of components among subsystems that perform different imaging and spectrometric functions. The input optics for the various functions would be mounted in a single optical head. With the exception of a targeting lens, the input optics would all be aimed at the same spot on a specimen, thereby both (1) eliminating the need to reposition the specimen to perform different imaging and/or spectroscopic observations and (2) ensuring that data from such observations can be correlated with respect to known positions on the specimen. The figure schematically depicts the principal components and subsystems of the instrument. The targeting lens would collect light into a multimode optical fiber, which would guide the light through a fiber-selection switch to a reflection/ fluorescence spectrometer. The switch would have four positions, enabling selection of spectrometer input from the targeting lens, from either of one or two multimode optical fibers coming from a reflectance/fluorescence- microspectrometer optical head, or from a dark calibration position (no fiber). The switch would be the only moving part within the instrument.

Mouroulis, Pantazis↗

Fiber optic sensors; Proceedings of the Meeting, Cannes, France, November 26, 27, 1985

The conference presents papers on distributed sensors and sensor networks, signal processing and detection techniques, temperature measurements, chemical sensors, and the measurement of pressure, strain, and displacements. Particular attention is given to optical fiber distributed sensors and sensor networks, tactile sensing in robotics using an optical network and Z-plane techniques, and a spontaneous Raman temperature sensor. Other topics include coherence in optical fiber gyroscopes, a high bandwidth two-phase flow void fraction fiber optic sensor, and a fiber-optic dark-field microbend sensor.

Arditty, Herve J.↗

Periodic weight support effects on rat soleus fibers after hindlimb suspension

The morphological and histochemical properties of rat soleus muscles were investigated following one week of hindlimb suspension. Short-duration daily weight support activity is shown to ameliorate, but not prevent, soleus atrophy induced by hindlimb suspension. The results indicate that fiber cross-sectional area is more responsive to periodic weight support in dark than light ATPas fibers, and that muscle fiber atrophy need not be associated with a loss in succinate dehydrogenase activity.

Hauschka, Edward O.↗

Fiber-Optic Strain Sensors With Linear Characteristics

Fiber-optic modal domain strain sensors having linear characteristics over wide range of strains proposed. Conceived in effort to improve older fiber-optic strain sensors. Linearity obtained by appropriate choice of design parameters. Pattern of light and dark areas at output end of optical fiber produced by interference between electromagnetic modes in which laser beam propagates in fiber. Photodetector monitors intensity at one point in pattern.

Egalon, Claudio O.↗

Few-Mode Fiber Coupled Superconducting Nanowire Single-Photon Detectors for Photon Efficient Optical Communications

The NASA Glenn Research Center's development of a high-photon efficiency real-time optical communications ground receiver has added superconducting nanowire single-photon detectors (SNSPDs) coupled with few-mode fibers (FMF). High data rate space-to-ground optical communication links require enhanced ground receiver sensitivity to reduce spacecraft transmitter constraints, and therefore require highly efficient coupling from fiber to detector. In the presence of atmospheric turbulence the received optical wave front can be severely distorted introducing higher-order spatial mode components to the received signal. To reduce mode filtering and mismatch loss and the resulting degradations to detector coupling efficiency, we explore the use of few-mode fiber coupling to commercial single-pixel SNSPDs. Graded index 20-µm few-mode fibers allow the commercial single pixel SNSPD's active area to couple with equal efficiency as single mode fibers. Here we determine detector characteristics such as count rate, detection efficiency, dark counts, and jitter, as well as detection efficiencies for higher-order fiber spatial modes. Additionally, we assess the laboratory performance of the detectors in an optical system which emulates future deep space optical communications links.

Vyhnalek, Brian E.↗

Physical aging and its influence on the reliability of network epoxies and epoxy-matrix composites

The matrix-dominated physical and mechanical properties of a carbon fiber reinforced epoxy composite and a neat epoxy resin were found to be affected by sub-Tg annealing in nitrogen and dark atmosphere. Postcured specimens of Thornel 300 carbon-fiber/Fiberite 934 epoxy as well as Fiberite 934 epoxy resin were quenched from above Tg and given annealing at 140 C, 110 C, or 80 C, for time up to one-hundred thousand minutes. No weight loss was observed during annealing at these temperatures. Significant variations were found in density, modulus, hardness, damping, moisture absorption ability, thermal expansivity. Moisture-epoxy interactious were also studied. The kinetics of aging as well as the molecular aggregation during this densification process were monitored by differential scanning calorimetry, dynamic mechanical analysis, density gradient column, microhardness tester, Instron, and solid-state nuclear magnetic resonance spectroscopy.

Heinemann, K.↗

Development in High-Density Cobra Fiber Positioners for the Subaru Telescope's Prime Focus Spectrometer

The Prime Focus Spectrograph (PFS) is a fiber fed multi-object spectrometer for the Subaru Telescope that will conduct a variety of targeted surveys for studies of dark energy, galaxy evolution, and galactic archaeology. The key to the instrument is a high density array of fiber positioners placed at the prime focus of the Subaru Telescope. The system, nicknamed "Cobra", will be capable of rapidly reconfiguring the array of 2394 optical fibers to the image positions of astronomical targets in the focal plane with high accuracy. The system uses 2394 individual "SCARA robot" mechanisms that are 7.7mm in diameter and use 2 piezo-electric rotary motors to individually position each of the optical fibers within its patrol region. Testing demonstrates that the Cobra positioner can be moved to within 5 micrometers of an astronomical target in 6 move iterations with a success rate of 95%. The Cobra system is a key aspect of PFS that will enable its unprecedented combination of high-multiplex factor and observing efficiency on the Subaru telescope. The requirements, design, and prototyping efforts for the fiber positioner system for the PFS are described here as are the plans for modular construction, assembly, integration, functional testing, and performance validation.

Cobra↗

Cobra Fiber-Optic Positioner Upgrade

A prime focus spectrometer (PFS), along with corrective optics, will mount in place of the secondary mirror of the Subaru telescope on Mauna Kea, Hawaii. This will allow simultaneous observations of cosmologic targets. It will enable large-scale galactic archeology and dark energy surveys to help unlock the secrets of the universe. To perform these cosmologic surveys, an array of 2,400 optical fibers needs to be independently positioned within the 498-mm-diameter focal plane of the PFS instrument to collect light from galaxies and stars for spectrographic analyses. To allow for independent re-positioning of the fibers, a very small positioner (7.7 mm in diameter) is required. One hundred percent coverage of the focal plane is also required, so these small actuators need to cover a patrol region of 9.5 mm in diameter. To optimize the amount of light that can be collected, the fibers need to be placed within 5 micrometers of their intended target (either a star or galaxy). The Cobra Fiber Positioner was designed to meet the size and accuracy requirements stated above. Cobra is a two-degrees-of-freedom mechanism that can position an optical fiber in the focal plane of the PFS instrument to a precision of 5 micrometers. It is a theta-phi style positioner containing two rotary piezo tube motors with one offset from the other, which enables the optic fibers to be placed anywhere in a small circular patrol region. The patrol region of the actuator is such that the array of 2,400 positioners allows for full coverage of the instrument focal plane by overlapping the patrol areas. A second-generation Cobra positioner was designed based on lessons learned from the original prototype built in 2009. Improvements were made to the precision of the ceramic motor parts, and hard stops were redesigned to minimize friction and prevent jamming. These changes resulted in reducing the number of move iterations required to position the optical fiber within 5 micrometers of its target. At the time of this reporting, there are still many tests to be performed that will validate system level performance, but on an individual level, the Cobra positioner demonstrates excellent performance and will enable the PFS instrument to make unprecedented measurements of the universe. What is unique about the upgrades made to the Cobra positioner is the improved performance due to the design changes in the hard stops and the ceramic end caps of the motors. Other changes were made to reduce the unit cost of a Cobra positioner without affecting the performance, since thousands of these devices will have to be built for the PFS instrument.

Fisher, Charles D.↗

Color Changing Material for Hydrogen Leak Detection

Kennedy Space Center scientists developed a hydrogen leak sensor utilizing a combination of chemochromic pigment and polymer that can be molded or fiber spun into rigid or flexible shapes such as tape. The sensor turns a dark color when exposed to hydrogen gas. This sensor has proven to be very effective for pinpointing the exact location of leaks in hydrogen gas lines and fittings at launch pads. Kennedy Space Center exclusively licensed this technology to the University of Central Florida (UCF), who also holds patents that are complimentary to KSC's. UCF has bundled the patents and exclusively licensed the portfolio to HySense Technology LLC, a startup company founded by a UCF professor who supports the UCF Florida Solar Energy Center (FSEC). HySense has fully developed its product (known as Intellipigment"TM"), and currently has five commercial customers. The company recently won the $100,000 first-place award at the CAT5 innovation competition at the Innovation Concourse of the Southeast: Safety & Manufacturing event in Orlando, FL. Commercial production and sales of this technology by HySense Technology will make this leak sensor widely available for use by NASA, DoD, and industries that utilize hydrogen gas.

Material for Hyrogen↗

Nulling Interferometry: Working on the Dark Fringe

Deep nulling of both laser and thermal radiation has been demonstrated in the laboratory at visible wavelengths, by means of a rotational shearing interferometer (RSI) coupled to single-mode input and output fibers.

nulling interferometry fringe technical reports le↗

The Electron Calorimeter (ECAL) Long Duration Balloon Experiment

Accurate measurements of the cosmic ray electron energy spectrum in the energy region 50 GeV to greater than 1 TeV may reveal structure caused by the annihilation of exotic dark matter particles and/or individual cosmic ray sources. Here we describe a new long duration balloon (LDB) experiment, ECAL, optimized to directly measure cosmic ray electrons up to several TeV. ECAL includes a double layer silicon matrix, a scintillating optical fiber track imager, a neutron detector and a fully active calorimeter to identify more than 90% of the incident electrons with an energy resolution of about 1.7% while misidentifying only 1 in 200,000 protons and 0.8% of secondary gamma rays as electrons. Two ECAL flights in Antarctica are planned for a total exposure of 50 days with the first flight anticipate for December 2009.

Guzik, T. G.↗

Kinetic separation of phototropism from blue-light inhibition of stem elongation

These experiments tested the hypothesis that phototropic bending arises when a light gradient across the stem differentially inhibits cell elongation because of direct inhibition of cell elongation by light (the Blaauw hypothesis). Continuous irradiation of dark-grown cucumber seedlings (Cucumis sativus L.) with unilateral blue light inhibited hypocotyl elongation within 30 s, but did not induce phototropic curvature until 4.5 h after the start of irradiation. Marking experiments showed that curvature began simultaneously at the top and bottom of the growing region. In situ measurements of the light gradient across the stem with a glass fiber optic indicated that a 5- to 6-fold difference in fluence rate was established on the two sides of the stem. The light gradient established at the start of irradiation was the same as that after 6 h of irradiation. Changes in gravitropic responsiveness during this period were also ruled out. Calculations show that the light gradient should have caused curvature which would be detectable within 30 to 60 min and which would extrapolate to the start of irradiation--if the Blaauw hypothesis were correct. The long lag for phototropism in this case indicates that rapid inhibition of cell elongation by blue light does not cause the asymmetrical growth of phototropism. Rather, phototropism is superimposed upon this separate light growth response.

NASA Discipline Number 40-30↗

Vacuum Nuller Testbed Performance, Characterization and Null Control

The Visible Nulling Coronagraph (VNC) can detect and characterize exoplanets with filled, segmented and sparse aperture telescopes, thereby spanning the choice of future internal coronagraph exoplanet missions. NASA/Goddard Space Flight Center (GSFC) has developed a Vacuum Nuller Testbed (VNT) to advance this approach, and assess and advance technologies needed to realize a VNC as a flight instrument. The VNT is an ultra-stable testbed operating at 15 Hz in vacuum. It consists of a MachZehnder nulling interferometer; modified with a "W" configuration to accommodate a hexpacked MEMS based deformable mirror (DM), coherent fiber bundle and achromatic phase shifters. The 2-output channels are imaged with a vacuum photon counting camera and conventional camera. Error-sensing and feedback to DM and delay line with control algorithms are implemented in a real-time architecture. The inherent advantage of the VNC is that it is its own interferometer and directly controls its errors by exploiting images from bright and dark channels simultaneously. Conservation of energy requires the sum total of the photon counts be conserved independent of the VNC state. Thus sensing and control bandwidth is limited by the target stars throughput, with the net effect that the higher bandwidth offloads stressing stability tolerances within the telescope. We report our recent progress with the VNT towards achieving an incremental sequence of contrast milestones of 10(exp 8) , 10(exp 9) and 10(exp 10) respectively at inner working angles approaching 2A/D. Discussed will be the optics, lab results, technologies, and null control. Shown will be evidence that the milestones have been achieved.

Lyon, R. G.↗