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At least 19 records

Utilization of potatoes for life support in space. V. Evaluation of cultivars in response to continuous light and high temperature

Twenty-four potato (Solanum tuberosum L.) cultivars from different regions of the world were evaluated in terms of their responses to continuous light (24 h photoperiod) and to high temperature (30 C) in two separate experiments under controlled environments. In each experiment, a first evaluation of the cultivars was made at day 35 after transplanting, at which time 12 cultivars exhibiting best growth and tuber initiation were selected. A final evaluation of the 12 cultivars was made after an additional 21 days of growth, at which time plant height, total dry weight, tuber dry weight, and tuber number were determined. In the continuous light evaluation, the 12 selected cultivars were Alaska 114, Atlantic, Bintje, Denali, Desiree, Haig, New York 81, Ottar, Rutt, Snogg, Snowchip, and Troll. In the high temperature evaluation, the 12 selected cultivars were Alpha, Atlantic, Bake King, Denali, Desiree, Haig, Kennebec, Norland, Russet Burbank, Rutt, Superior, and Troll. Among the cultivars selected under continuous irradiation, Desiree, Ottar, Haig, Rutt, Denali and Alaska showed the best potential for high productivity whereas New York 81 and Bintje showed the least production capability. Among the cultivars selected under high temperature, Rutt, Haig, Troll and Bake King had best performance whereas Atlantic, Alpha, Kennebec and Russet Burbank exhibited the least production potential. Thus, Haig and Rutt were the two cultivars that performed well under continuous irradiation and high temperature conditions, and could have maximum potential for adaptation to varying stress environments. These two cultivars may have the best potential for use in future space farming in which continuous light and/or high temperature conditions may exist. However, cultivar responses under combined conditions of continuous light and high temperature remains for further validation.

NASA Discipline Life Support Systems↗

Selection and Characterization of Vegetable Crop Cultivars for use in Advanced Life Support Systems

Cultivar evaluation for controlled environments is a lengthy and multifaceted activity. The chapters of this thesis cover eight steps preparatory to yield trials, and the final step of cultivar selection after data are collected. The steps are as follows: 1. Examination of the literature on the crop and crop cultivars to assess the state of knowledge. 2. Selection of standard cultivars with which to explore crop response to major growth factors and determine set points for screening and, later, production. 3. Determination of practical growing techniques for the crop in controlled environments. 4. Design of experiments for determination of crop responses to the major growth factors, with particular emphasis on photoperiod, daily light integral and air temperature. 5. Developing a way of measuring yield appropriate to the crop type by sampling through the harvest period and calculating a productivity function. 6. Narrowing down the pool of cultivars and breeding lines according to a set of criteria and breeding history. 7. Determination of environmental set points for cultivar evaluation through calculating production cost as a function of set points and size of target facility. 8. Design of screening and yield trial experiments emphasizing efficient use of space. 9. Final evaluation of cultivars after data collection, in terms of production cost and value to the consumer. For each of the steps, relevant issues are addressed. In selecting standards to determine set points for screening, set points that optimize cost of production for the standards may not be applicable to all cultivars. Production of uniform and equivalent- sized seedlings is considered as a means of countering possible differences in seed vigor. Issues of spacing and re-spacing are also discussed.

Langhans, Robert W.↗

Selection of Cultivars for use in Controlled Environment Life Support System (CELSS) Human Rated Test Facility (HRTF) Trials

The aims under this training grant, as under the subsequent fellowship, were to elaborate the theory and technique of cultivar evaluation for specialized controlled environments, then to employ the technique on selected crops, ultimately conducting cultivar trials, and making the knowledge gained available for use in NASA's space program. We undertook a comprehensive search of the Cornell agricultural library (Mann library) and its data-bases for any and all material relating to cultivar evaluation of vegetable crops, and also developed the logic of how to go about narrowing down the field of contending cultivars when undertaking cultivar trials. The results of this work, the principal outcome of the grant, are reflected in his MS thesis, particularly in Chapter 2, "Commercial and Scientific Literature," and even more so in Chapter 8, "Selecting cultivars and lines for screening." David also attended annual conferences of vegetable crop plant breeders, annual yield trials and breeding trials for vegetable crops, as well as relevant professional conferences such as the ASHS annual meetings, and the others.

Langhans, Robert W.↗

Dwarf Tomato and Pepper Cultivars for Space Crops

Several dwarf tomato and pepper varieties were evaluated under ISS-simulated growth conditions (22ºC, 50% RH, 1500 ppm CO2, and 300 μmol m(exp -2) s(exp -1) of light for 16 h per day) with the goal of selecting those with the best growth, nutrition, and organoleptic potential for use in a pick and eat salad crop system on ISS and future exploration flights. Testing included six cultivars of tomato (Red Robin, Scarlet Sweet ’N’ Neat, Tiny Tim, Mohamed, Patio Princess, and Tumbler) and six cultivars of pepper (Red Skin, Fruit Basket, Cajun Belle, Chablis, Sweet Pickle, and Pompeii). Plants were grown to an age sufficient to produce fruit (70 to 106 days for tomato and 109 days for pepper). Tomato fruits were harvested when they showed full red color, beginning ca. 70-days age and then at weekly intervals thereafter, while peppers were grown until numerous fruits showed color and all fruits (green and colored) were harvested once at the end of the test. Plant sizes, yields, and nutritional attributes were measured and used to down-select to three cultivars for each species. In particular, we were interested in cultivars that were short (dwarf) but still produced high yields. Nutritional data included elemental (Ca, Mg, Fe, and K) composition, vitamin K, phenolics, lycopene, anthocyanin, lutein, and zeaxanthin. The three down-selected cultivars for each species were evaluated for sensory attributes, including overall acceptability, appearance, color intensity aroma, flavor and texture. The combined data were compared and given weighting factors to rank the cultivars as potential candidates for testing in space. For tomato, the ranking was 1) cv. Mohamed, 2) cv. Red Robin, and 3) cv. Sweet N’ Neat. For pepper, the ranking was 1) cv. Pompeii, 2) cv. Red Skin, and 3) cv. Fruit Basket. These rankings are somewhat subjective but provide a good starting point for conducting higher fidelity testing with these crops (e.g., testing with LED lighting similar to the Veggie plant unit), and ultimately conducting flight experiments.

Spencer, LaShelle E.↗

Growth of Three Lettuce Cultivars in NASA's HDU PEM During the 2010 DRATS Test

NASA's 2010 Desert Research and Technology Studies (DRATS) of the VEGGIE Food Production System in the Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) was the first operational evaluation of salad crop production technology in a NASA analog test. Rooting media and slow release fertilizers were evaluated for three lettuce cultivars that had shown promise as candidates for a surface based food production system. These tests involved comparing growth, color and quality of the lettuce cultivars grown under VEGGIE LED array (Orbitec, Madison, WI) or Biomass Production System for Education ((BSEe), Orbitec, Madison, WI) compact fluorescent lamps using a gravity feed water delivery system. Mission relevant conditions of CO2, temperature and RH were maintained using controlled environment chambers (EGC, Chagrin Falls, OH). Growth data was obtained for the two red leaf lettuce cultivars, Outredgeous and Firecracker, and the green Bibb lettuce cultivar, Flandria. Growth and quality was evaluated using different concentrations (7.5 g/L and 15 g/L) of commercial slow release fertilizer (Osmocote Plus 15-9-12, Scotts, Maryville, OH) and Nutricote 18-6-8 (Florikan, Sarasota, FL) in either a peat/vermiculite media (sunshine LP5 MiX, Sungro, Bellview, WA) or calcined montmorillonite clay [(arcillite,)Turface Proleague, Profile LLC, Buffalo Grove, IL]. The commercial peat/vermiculite mix generally resulted in larger plants than those grown in arcillite. Increasing the concentration of Osmocote from 7.5 to 15 g/L increased the height, dry mass, and leaf area of lettuce cultivars. In contrast, there was a decrease in growth parameters when concentration of Nutricote was increased from 7.5 to 15 g/L. The best growth was obtained with the 7.5 g/L Nutricote using a commercial peat/vermiculite mixture. This media was used for field testing VEGGIE plant system in the 2010 DRAT test. The VEGGIE nutrient delivery system worked well, was able to be maintained by multiple operators with a minimum of training, and supported excellent lettuce growth for the duration of the 14-day test. The operational DRAT field testing in the HDU identified light quality issues related to morphology and pigment development that will need to be addressed through additional testing. Feedback from the crew, ground support personnel, and human factors leads was uniformly positive on the psychological value of having the crop production system in the pressurized excursion module. Data are being used to design a plant atrium with LED lighting to evaluate salad crop growth during NASA's 2011 DRATS test.

Stutte, Gary W.↗

LAI Assimilation Schedules to Constrain Uncertain Cultivars and Soils in CERES-Maize

In anticipation of large-domain crop model applications where precise local configuration and calibration is not possible, we describe benefits and potential drawbacks of employing a crop pest module to achieve leaf area index (LAI) assimilation into a high performing CERES-Maize crop model configuration at a field experiment site in Perry, Iowa. Simulation experiments explore the use of MODIS satellite-derived LAI to constrain and adjust LAI to counter imprecise cultivar and soil configurations often occurring in the absence of high-quality local information. Simulations using single-day, window, and continuous LAI replacement across 5 cultivars and 2 soil calibration approaches for nine corn rotation years from 2004 to 2020 led to different yield outcomes and reverberations throughout the field environment. Evaluating variance and mean bias, results indicate minimal interventions in early vegetative and grain-filling stages were more beneficial than use of continuous LAI adjustments, as they minimized disruptions to the internal resource balances governing plant stresses and grain production. LAI adjustment was particularly helpful in constraining growth related to uncertain thermal unit requirements and leaf tip appearance rates (P1 and PHINT cultivar parameters, respectively). Findings underscore the need to assimilate additional state variables to ensure internal biophysical coherence. This approach shows promise for applications spanning wider domains with prediction time pressure where detailed configuration, more complex assimilation methods, or recalibration of crop model parameters may not be practical.

Phenology↗

Dwarf Tomato and Pepper Cultivars for Space Crops

Several cultivars of dwarf tomatoes and dwarf peppers were studied as possible candidate for space crops. Results showed the tomato cvs. Red Robin, Mohamed, and Sweet 'N' Neat produced the greatest yields, while pepper cvs. Pompeii, Red Skin, and Fruit Basket produced the greatest yields. The tomato and pepper cultivars were also analyzed by taste panels for organoleptic attributes, and all the cultivars were found to be acceptable by the taste panelists.

Spencer, LaShelle↗

The Effect of Plant Cultivar, Growth Media, Harvest Method and Post Harvest Treatment on the Microbiology of Edible Crops

Systems for the growth of crops in closed environments are being developed and tested for potential use in space applications to provide a source of fresh food. Plant growth conditions, growth media composition and harvest methods can have an effect on the microbial population of the plant, and therefore should be considered along with the optimization of plant growth and harvest yields to ensure a safe and palatable food crop. This work examines the effect of plant cultivar, growth media, and harvest method on plant microbial populations. Twelve varieties of leafy greens and herbs were grown on a mixture of Fafard #2 and Arcillite in the pillow root containment system currently being considered for the VEGGIE plant growth unit developed by Orbitec. In addition, ,Sierra and Outredgeous lettuce varieties were grown in three different mixtures (Fafard #2, Ardllite, and Perlite/Vermiculite). The plants were analyzed for microbial density. Two harvest methods, "cut and come again" (CACA) and terminal harvest were also compared. In one set ofexpe'riments red leaf lettuce and mizuna were grown in pots in a Biomass Production System for education. Plants were harvested every two weeks by either method. Another set of experiments was performed using the rooting pillows to grow 5 varieties of leafy greens and cut harvesting at different intervals. Radishes were harvested and replanted at two-week intervals. Results indicate up to a 3 IOglO difference in microbial counts between some varieties of plants. Rooting medium resulted in an approximately 2 IOglO lower count in the lettuce grown in arscillite then those grown in the other mixtures. Harvest method and frequency had less impact on microbial counts only showing a significant increase in one variety of plant. Post harvest methods to decrease the bacterial counts on edible crops were investigated in these and other experiments. The effectiveness of PRO-SAN and UV-C radiation is compared.

Hummerick, Mary P.↗

Reflectance differences between Target and Torch rape cultivars

Spectroradiometric reflectance measurements were made on Target and Torch plants (four and five leaves, respectively) that were growing in 0.09 m2 soil-containing flats. Torch's spectrophotometric single leaf reflectance was consistently lower than Target's at the 650-nm chlorophyll absorption band because Torch's chlorophyll concentration was larger than Target's, which caused more red light absorption. Spectroradiometric measurements indicate that: wet soil strongly absorbs visible light (500 to 700 nm) so that Target's soil-containing flat with 60% plant cover has less reflectance than Torch's soil-containing flat with 75% plant cover; Torch (most foiliage) has higher near-infrared (750 to 1,350 nm) reflectance than Target (least foliage); and the 2,200-nm wavelength is a candidate band to distinguish Target from Torch. The difference in chlorophyll concentrations between Target and Torch, compared with leaf structural differences, is apparently the most important factor that would affect the infrared color film's tonal response to vegetation in the photographic sensitive region (500 to 900 nm).

Gausman, H. W.↗

Utilization of potatoes for life support systems in space. I - Cultivar-photoperiod interactions

The productive potential of four potato varieties considered as a food source for space stations and/or lunar colonies was assessed for plants grown for 15 weeks in walk-in rooms at 20 C under 12-, 16-, or 20-hr exposures to 400-micromol/sq m per sec photosynthetic photon flux. It was found that Norland potatoes yielded the greatest tuber fresh weight, producing 2.3, 2.4, and 2.9 kg/plant under 12-, 16, and 20-hr, respectively. The respective yields for the other three varieties were: Superior, 1.9, 1.5, and 1.8 kg/plant; Norchip, 1.8, 1.4, and 2.0 kg/plant; and Kennebec, 2.3, 0.2, and 0.8 kg/plant.

Wheeler, Raymond M.↗

Pick and Eat Crop Testing: Dwarf Tomato and Pepper as Candidate Space Crops

Several dwarf tomato and pepper varieties were evaluated under International Space Station (ISS)-simulated growth conditions (22 degrees Centigrade, 50 percent relative humidity, 1500 parts per million CO2, and 300 micromoles per square meter per second of light for 16 hours per day) with the goal of selecting those with the best growth, nutrition, and organoleptic potential for use in a pick and eat salad crop system on ISS and future exploration flights. Testing included six cultivars of tomato (Red Robin, Scarlet Sweet 'N' Neat, Tiny Tim, Mohamed, Patio Princess, and Tumbler) and six cultivars of pepper (Red Skin, Fruit Basket, Cajun Belle, Chablis, Sweet Pickle, and Pompeii). Plants were grown to an age sufficient to produce fruit (up to 106 days for tomato and 109 days for pepper) using Turface (arcillite) potting media with 18-6-8 control-release fertilizer and supplemental nutrient solution beginning around 60-days-age. Tomato fruits were harvested when they showed full red color, beginning around 70-days age and then at weekly intervals thereafter, while peppers were grown until fruits showed color and were harvested twice (first test) and just once at the end of the second test, with the final harvests including colored and green fruit. Plant sizes, yields, and nutritional attributes were measured and used to down-select to three cultivars for each species. In particular, we were interested in cultivars that were short (dwarf) but still produced high yields. Nutritional data included elemental (Ca, Mg, Fe, and K) content, vitamin K, phenolics, lycopene (for tomato), anthocyanin, lutein, and zeaxanthin. The three down-selected cultivars for each species were grown again and the harvested fruit sent to NASA's Johnson Space Center for sensory evaluation, which included overall acceptability, appearance, color intensity, aroma, flavor and texture. The combined data were compared and given weighting factors to rank the cultivars as candidates for testing in space. Weightings gave maximum importance to plant size (smaller being good) and fruit yield (greater yields being good). For tomato, the ranking was 1) cultivar Mohamed and cultivar Red Robin (tied), and 3) cultivar Sweet N' Neat. For pepper, the ranking was 1) cultivar Pompeii, 2) cultivar Red Skin, and 3) cultivar Fruit Basket. These rankings are somewhat subjective but provide a starting point for conducting higher fidelity testing with these crops (e.g., testing with light emitting diode lighting similar to the Veggie plant unit on ISS), and ultimately conducting a flight experiment.

Food↗

Correlation between calmodulin activity and gravitropic sensitivity in primary roots of maize

Recent evidence indicates a role for calcium and calmodulin in the gravitropic response of primary roots of maize (Zea mays, L.). We examined this possibility by testing the relationship between calmodulin activity and gravitropic sensitivity in roots of the maize cultivars Merit and B73 x Missouri 17. Roots of the Merit cultivar require light to the gravitropically competent. The gravitropic response of the Missouri cultivar is independent of light. The occurrence of calmodulin in primary roots of these maize cultivars was tested by affinity gel chromatography followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with bovine brain calmodulin as standard. The distribution of calmodulin activity was measured using both the phosphodiesterase and NAD kinase assays for calmodulin. These assays were performed on whole tissue segments, crude extracts, and purified extracts. In light-grown seedlings of the Merit cultivar or in either dark- or light-grown seedlings of the Missouri cultivar, calmodulin activity per millimeter of root tissue was about 4-fold higher in the apical millimeter than in the subtending 3 millimeters. Calmodulin activity was very low in the apical millimeter of roots of dark-grown (gravitropically nonresponsive) seedlings of the Merit cultivar. Upon illumination, the calmodulin activity in the apical millimeter increased to a level comparable to that of light-grown seedlings and the roots became gravitropically competent. The time course of the development of gravitropic sensitivity following illumination paralleled the time course of the increase in calmodulin activity in the apical millimeter of the root. The results are consistent with the suggestion that calmodulin plays an important role in the gravitropic response of roots.

NASA Program Space Biology↗

Assessing solar energy and water use efficiencies in winter wheat

The water use and solar energy conversion efficiencies of two cultivars of winter wheat (Triticum aestivum L., vars, Centurk and Newton) planted at three densities, were examined during a growing season. Water use, based on soil moisture depletion, was the lowest under the light, and the highest under the heavy planting densities of both cultivars. Water use efficiency of medium and heavy planting densities were greater than the light planting densities in both cultivars. The canopy radiation extinction coefficients of both cultivars increased with increases in planting density. Efficiency of operation interception of photosynthetically active radiation by both cultivars improved from the time of jointing until anthesis, and then decreased during senescence. The efficiency of the conversion of intercepted radiation to dry matter (biochemical efficiency) decreased throughout the growing season both cultivars. The interception, biochemical, and photosynthetic efficiencies improved as planting density increased.

Asrar, G.↗

Media Effects on Lettuce Growth in "Pillows" Designed for the VEGGIE Spaceflight Growth Chamber

VEGGIE is a prototype vegetable production unit for space designed by Orbital Technologies Corporation that is being developed to fly on the International Space Station. A modular plant rooting system "pillow" is being designed to support plant growth in VEGGIE under microgravity conditions. VEGGIE pillows are small self-contained packets of media with time-release fertilizer that can wick water passively from a root mat reservoir. Seeds are planted in pillows and the entire root system of a plant is contained as the crop develops, preventing loss into the spacecraft cabin. This study compared five media types and three lettuce cultivars in pillows growing in a VEGGIE analog environment.. Media consisted of a peat-based potting mix (Fafard #2,Conrad Fafard Inc., Agawam, MA), and a calcined clay, (arcillite, 1-2 mm sifted, Turface Proleague, Profile LLC, Buffalo Grove IL) as well as three different blends of the two, 70:30, 50:50, and 30:70. Lettuce cultivars tested were 'Sierra', a bi-colored French crisp Batavia lettuce, 'Outredgeous', a red romaine lettuce and 'Flandria', a green butter head variety. Plants were grown for 28 days, harvested, biometric data was obtained, and tissue mineral analysis was performed. For all cultivars, lettuce plants grown in the media blends were more productive than those in the individual media types. All cultivars showed bell-shaped curves in response to increases in arcillite / decreases in Fafard #2 for leaf area, fresh, and dry mass. Plants in 100% Fafard #2 and in 100% arcillite were stunted, but only those in higher levels of Fafard #2 (70% and 100%) had reduced shoot percent moisture, possibly indicating that mechanisms causing stunting differed. Variation in tissue nutrient content are consistent with this, with Mg and Mn highest in plants grown in 100% Fafard and decreasing as the concentration of arcillite increased. Color also varied with media, especially in the 'Sierra' lettuce, with plants grown in increasing levels of Fafard #2 being much more red and those in 100% arcillite almost completely green. The red-leaf cultivar 'Outredgeous' showed increasing chlorophyll (SPAD values) with increasing percentage of arcillite. In all cultivars tested it appears that a mixture of media types, usually 50:50 or 30:70 Fafard #2: arcillite, sustained healthier, more productive plants. Smaller, less productive plants in either of the individual media may indicate stress issues, however more work is needed to understand the reasons for this sub-optimal growth. This work was supported by NASA

Massa, Gioia↗

Diversity Buffers Winegrowing Regions from Climate Change Losses

Agrobiodiversity—the variation within agricultural plants, animals, and practices—is often suggested as a way to mitigate the negative impacts of climate change on crops [S. A. Wood et al., Trends Ecol. Evol. 30, 531–539 (2015)]. Recently, increasing research and attention has focused on exploiting the intraspecific genetic variation within a crop [Hajjar et al., Agric. Ecosyst. Environ. 123, 261–270 (2008)], despite few relevant tests of how this diversity modifies agricultural forecasts. Here, we quantify how intraspecific diversity, via cultivars, changes global projections of growing areas. We focus on a crop that spans diverse climates, has the necessary records, and is clearly impacted by climate change: winegrapes (predominantly Vitis vinifera subspecies vinifera). We draw on long-term French records to extrapolate globally for 11 cultivars (varieties) with high diversity in a key trait for climate change adaptation—phenology. We compared scenarios where growers shift to more climatically suitable cultivars as the climate warms or do not change cultivars. We find that cultivar diversity more than halved projected losses of current winegrowing areas under a 2 °C warming scenario, decreasing areas lost from 56 to 24%. These benefits are more muted at higher warming scenarios, reducing areas lost by a third at 4 °C (85% versus 58%). Our results support the potential of in situ shifting of cultivars to adapt agriculture to climate change—including in major winegrowing regions—as long as efforts to avoid higher warming scenarios are successful.

Morales-Castilla, Ignacio↗

A proposal to demonstrate production of salad crops in the space station mockup facility with particular attention to space, energy, and labor constraints

A desire for fresh vegetables for consumption during long term space missions has been foreseen. To meet this need in a microgravity environment within the limited space and energy available on Space Station requires highly productive vegetable cultivars of short stature to optimize vegetable production per volume available. Special water and nutrient delivery systems must also be utilized. As a first step towards fresh vegetable production in the microgravity of Space Station, several soil-less capillary action media were evaluated for the ability to support growth of two root crops (radish and carrot) which are under consideration for inclusion in a semi-automated system for production of salad vegetables in a microgravity environment (Salad Machine). In addition, productivity of different cultivars of radish was evaluated as well as the effect of planting density and cultivar on carrot production and size. Red Prince radish was more productive than Cherry Belle and grew best on Jiffy Mix Plus. During greenhouse studies, vermiculite and rock wool supported radish growth to a lesser degree than Jiffy Mix Plus but more than Cellular Rooting Sponge. Comparison of three carrot cultivars (Planet, Short n Sweet, and Goldinhart) and three planting densities revealed that Short n Sweet planted at 25.6 sq cm/plant had the greatest root fresh weight per pot, the shortest mean top length, and intermediate values of root length and top fresh weight per pot. Red Prince radish and Short n Sweet carrot showed potential as productive cultivars for use in a Salad Machine. Results of experiments with solid capillary action media were disappointing. Further research must be done to identify a solid style capillary action media which can productively support growth of root crops such as carrot and radish.

Brooks, Carolyn A.↗