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At least 19 records

The Community Radiative Transfer Model (CRTM): Community-Focused Collaborative Model Development Accelerating Research to Operations

The Joint Center for Satellite Data Assimilation (JCSDA) Community Radiative Transfer Model (CRTM) is a fast, 1-D radiative transfer model used in numerical weather prediction, calibration/validation, etc. across multiple federal agencies and universities. The key benefit of the CRTM is that it is a satellite simulator. It provides a highly accurate representation of satellite radiances by using the specific sensor response functions convolved with a line-by-line radiative transfer model (LBLRTM). CRTM covers the spectral ranges consistent with all present operational and most research satellites, from visible to microwave. The capability to simulate ultraviolet radiances and support space-based radar sensors is being added over the next two years in CRTM Version 3.0. In addition to simulated radiances, the CRTM also provides Jacobian outputs needed to interpret satellite observations for numerical weather prediction. The Jacobian estimates how changes in geophysical parameters affect simulated measurements from satellite sensors. Using the Jacobian in modeling and weather prediction improves the accuracy and efficiency of data analysis, leading to better weather predictions. The CRTM model's success and growth depend on community contributions and evaluation. To facilitate this, we have made the CRTM highly accessible through modular programming, clear documentation and tutorials, public domain licensing, unfettered public access via Github, and a clear path to operational implementation for innovative research. We encourage and welcome contributions from the community to help us continue to improve the CRTM.

Benjamin T Johnson

Response of soybean rhizosphere communities to human hygiene water addition as determined by community level physiological profiling (CLPP) and terminal restriction fragment length polymorphism (TRFLP) analysis

In this report, we describe an experiment conducted at Kennedy Space Center in the biomass production chamber (BPC) using soybean plants for purification and processing of human hygiene water. Specifically, we tested whether it was possible to detect changes in the root-associated bacterial assemblage of the plants and ultimately to identify the specific microorganism(s) which differed when plants were exposed to hygiene water and other hydroponic media. Plants were grown in hydroponics media corresponding to four different treatments: control (Hoagland's solution), artificial gray water (Hoagland's+surfactant), filtered gray water collected from human subjects on site, and unfiltered gray water. Differences in rhizosphere microbial populations in all experimental treatments were observed when compared to the control treatment using both community level physiological profiles (BIOLOG) and molecular fingerprinting of 16S rRNA genes by terminal restriction fragment length polymorphism analysis (TRFLP). Furthermore, screening of a clonal library of 16S rRNA genes by TRFLP yielded nearly full length SSU genes associated with the various treatments. Most 16S rRNA genes were affiliated with the Klebsiella, Pseudomonas, Variovorax, Burkholderia, Bordetella and Isosphaera groups. This molecular approach demonstrated the ability to rapidly detect and identify microorganisms unique to experimental treatments and provides a means to fingerprint microbial communities in the biosystems being developed at NASA for optimizing advanced life support operations.

NASA Discipline Life Support Systems

Assessment of Soil Moisture Data Requirements by the Potential SMAP Data User Community: Review of SMAP Mission User Community

NASA's Soil Moisture Active and Passive (SMAP) mission is planned for launch in October 2014 and will provide global measurements of soil moisture and freeze thaw state. The project is driven by both basic research and applied science goals. Understanding how application driven end-users will apply SMAP data, prior to the satellite's launch, is an important goal of NASA's applied science program and SMAP mission success. Because SMAP data are unique, there are no direct proxy data sets that can be used in research and operational studies to determine how the data will interact with existing processes. The objective of this study is to solicit data requirements, accuracy needs, and current understanding of the SMAP mission from the potential user community. This study showed that the data to be provided by the SMAP mission did substantially meet the user community needs. Although there was a broad distribution of requirements stated, the SMAP mission fit within these requirements.

applications

Continuing to Build a Community Consensus on the Future of Human Space Flight: Report of the Fourth Community Workshop on Achievability and Sustainability of Human Exploration of Mars (AM IV)

To continue to build broadly based consensus on the future of human space exploration, the Fourth Community Workshop on Achievability and Sustainability of Human Exploration of Mars (AM IV), organized by Explore Mars, Inc. and the American Astronautical Society, was held at the Double Tree Inn in Monrovia, CA., December 68, 2016. Approximately 60 invited professionals from the industrial and commercial sectors, academia, and NASA, along with international colleagues, participated in the workshop. These individuals were chosen to be representative of the breadth of interests in astronaut and robotic Mars exploration.

Human Exploration

An Evening with NASA Scientists and Engineers: A Senior Living Community Outreach Program

Introduction: An Evening with NASA Scientists and Engineers is an outreach program for senior living communities, where participants learn from and interact with a scientist or engineer in a virtual setting. Participants learn about NASA science and engineering through a behind-the-scenes look from the people who work at NASA. The program focuses on both the stories behind the discoveries and the stories behind the people. The audience tunes in to the events via the NASA Zoom Webinar platform, where they have the opportunity to ask questions throughout the approximately 30-minute program. By bringing the audience into the conversation, we build rapport and thus improve their connection with NASA. Overview: An Evening with NASA Scientists and Engineers began in September 2020 when we identified a need for outreach to this particular demographic, as senior living communities had been hit especially hard by the COVID-19 pandemic. The program covers a wide range of topics, including heliophysics, astrophysics, planetary science, Earth science, planetary defense, and more, depending on what is topically appropriate at the time, and occurs on a roughly monthly basis. Each interactive presentation is between 20-25 minutes with 5-10 minutes of dedicated Q&A. Future dates and topics may change depending on new developments to NASA missions and stories. Audience: As of December 2021, we have four regularly attending senior living communities, spanning the East Coast from New Hampshire to Florida, with each joining the overall program at various stages of its development. The ability to reach many different senior living communities is crucial so that a diversity of people may benefit from this program. The audience consists of seniors with varying experience and comprehension levels – some are very familiar with the sciences while others are simply curious to learn more. Residents tune in to each event from their individual rooms or from their larger community rooms. RiverWoods Exeter. The RiverWoods Exeter community was our initial audience who acted as our “guinea pig” to see how this program might be implemented and to work out any major issues. They are located in Exeter, NH but also have two additional campuses in Durham, NH and Manchester, NH, that joined us a few months into the program. Culpepper Garden. Our second community to join the program was Culpepper Garden, a senior living community in Arlington, VA that provides both independent and assisted living housing to low-income seniors. Edinburgh Square. Our third community, Edinburgh Square, connected with us after hearing about the program through Culpepper Garden. Edinburgh Square is an HUD-subsidized retirement community in Roanoke, VA that houses both seniors and those living with disabilities. Moonstruck Astronomy Club. The Moonstruck Astronomy Club consists of residents from the On Top of the World retirement community in Ocala, FL and was the latest senior living community to join us. NASA Zoom Webinar: An Evening with NASA Scientists and Engineers reaches numerous senior living communities through live presentations that we host using the NASA Zoom Webinar virtual platform. Participants are typically already familiar with the Zoom platform which enables a lower barrier to entry. A recording of each talk is added to a designated video playlist on the NASA STEM YouTube channel so that audiences worldwide may view them [1]. These recordings began with our April 1, 2021 presentation, as this was when NASA Zoom Webinar was implemented, thus enabling us to record each talk. Metrics: We record attendance numbers from the senior living communities for each event as well as those from YouTube views after the presentation recording is posted online. Depending on which communities tune in, typical attendance numbers are between 60-100 people. RiverWoods Exeter has the most regularly attending residents, with an average of 55 attendees per event. As of December 2021, we have hosted fourteen events, nine of which were recorded and posted to our designated NASA STEM YouTube Channel playlist. The nine recordings combined have roughly 4,000 YouTube views thus far, with specific videos seeing higher view counts around launch dates (e.g. NASA’s DART mission) and trending topics (e.g. JWST). A list of speakers, topics, and links to presentation recordings is shown in Table 1. Future Program Expansion: Thus far, we have been adding communities on an individual basis. To make this process more streamlined, we are looking into working with senior living community networks and organizations such as LeadingAge [2]. In this manner, we will be able to reach more communities while also making it easier to send and receive relevant information.

S L Tiedeken

Impact of dilution on microbial community structure and functional potential: comparison of numerical simulations and batch culture experiments

A series of microcosm experiments was performed using serial dilutions of a sewage microbial community to inoculate a set of batch cultures in sterile sewage. After inoculation, the dilution-defined communities were allowed to regrow for several days and a number of community attributes were measured in the regrown assemblages. Based upon a set of numerical simulations, community structure was expected to differ along the dilution gradient; the greatest differences in structure were anticipated between the undiluted-low-dilution communities and the communities regrown from the very dilute (more than 10(-4)) inocula. Furthermore, some differences were expected among the lower-dilution treatments (e.g., between undiluted and 10(-1)) depending upon the evenness of the original community. In general, each of the procedures used to examine the experimental community structures separated the communities into at least two, often three, distinct groups. The groupings were consistent with the simulated dilution of a mixture of organisms with a very uneven distribution. Significant differences in community structure were detected with genetic (amplified fragment length polymorphism and terminal restriction fragment length polymorphism), physiological (community level physiological profiling), and culture-based (colony morphology on R2A agar) measurements. Along with differences in community structure, differences in community size (acridine orange direct counting), composition (ratio of sewage medium counts to R2A counts, monitoring of each colony morphology across the treatments), and metabolic redundancy (i.e., generalist versus specialist) were also observed, suggesting that the differences in structure and diversity of communities maintained in the same environment can be manifested as differences in community organization and function.

Non-NASA Center

The Influence of Ecological Isolation on the Structural and Functional Stability of Complex Microbial Communities

To help understand how the behavior of microorganisms and microbial communities in insular space habitats may differ from the behavior of these groups on Earth, long-term incubations (100+ days) were conducting using wastewater bioreactors (batch fed) designed to mimic "closed" and "open" ecological systems. The issue of immigration was considered, and the goal of the research was to determine whether the stability of microbial communities in space is reduced due to their prolonged isolation. Bioreactors were established by inoculating flasks of sterile synthetic wastewater with the microbial community obtained from a local treatment facility; each day, one-third of the medium in the flask was replaced with an equal volume of sterile artificial wastewater. Flasks were divided into two treatments: "closed" and "open" to recruitment of additional microorganisms. "Closed" flasks were maintained as described above, while the medium used to feed the "open" flasks was supplemented daily with a small amount of raw sewage (which provided a continuous source of new potential community members). Significant differences in microbial community structure and function developed in the two sets of communities, and the results suggest that the open community was more stable and better able to adjust to changing environmental conditions. Each community's resistance to environmental (temperature fluctuations) and biological stresses (starvation and invasion by an opportunistic pathogen Pseudomonas aeruginosa) was monitored. Experiments were also conducted to determine whether the effect of isolation changes depending on the microbial communities' initial diversity or composition; communities with a low(er) initial diversity were less stable. Overall, the results indicate that isolation will be an important factor influencing the activity of microbial communities on board spacecraft. A possible way of mitigating these effects would be to include communities with high initial diversity or to periodically re-inoculate the systems using diverse inocula transported from Earth.

Franklin, R. B.

The Effect of Dilution on the Structure of Microbial Communities

To determine how dilution of microbial communities affects the diversity of the diluted assemblage a series of numerical simulations were conducted that determined the theoretical change in diversity, richness, and evenness of the community with serial dilution. The results of the simulation suggested that the effects are non linear with a high degree of dependence on the initial evenness of the community being diluted. A series of incubation experiments using a range of dilutions of raw sewage as an inoculum into sterile sewage was used for comparison to the simulations. The diluted communities were maintained in batch fed reactors (three day retention time) for nine days. The communities were harvested and examined by conventional plating and by molecular analysis of the whole-community DNA using AFLP and T-RFLP. Additional, CLPP analysis was also applied. The effects on richness predicted by the numerical simulations were confirmed by the analyses used. The diluted communities fell into three groups, a low dilution, intermediate dilution, and high dilution group, which corresponded well with the groupings obtained for community richness in simulation. The grouping demonstrated the non-linear nature of dilution of whole communities. Furthermore, the results implied that the undiluted community consisted of a few dominant types accompanied by a number of rare (low abundance) types as is typical in unevenly distributed communities.

Mills, Aaron L.

Adaptation to environmental extremes structures functional traits in biological soil crust and hypolithic microbial communities

Biological soil crusts (biocrusts) are widespread in drylands and deserts. At the microhabitat scale, they also host hypolithic communities that live under semi-translucent stones. Both environmental niches experience exposure to extreme conditions such as high UV radiation, desiccation, temperature fluctuations, and resource limitation. However, hypolithic communities are somewhat protected from extremes relative to biocrust communities. Conditions are otherwise similar, so comparing them can answer outstanding questions regarding adaptations to environmental extremes. Using metagenomic sequencing, we assessed the functional potential of dryland soil communities and identified the functional underpinnings of ecological niche differentiation in biocrusts versus hypoliths. We also determined the effect of the anchoring photoautotroph (moss or cyanobacteria). Genes and pathways differing in abundance between biocrusts and hypoliths indicate that biocrust communities adapt to the higher levels of UV radiation, desiccation, and temperature extremes through an increased ability to repair damaged DNA, sense and respond to environmental stimuli, and interact with other community members and the environment. Intracellular competition appears to be crucial to both communities, with biocrust communities waging war using the Type VI Secretion System (T6SS) and hypoliths favoring diversity of antibiotics. The dominant primary producer had a reduced effect on community functional potential compared with niche, but an abundance of genes related to monosaccharide, amino acid, and osmoprotectant uptake in moss-dominated communities indicates reliance on resources provided to heterotrophs by mosses. Our findings indicate that functional traits in dryland communities are driven by adaptations to extremes and we identify strategies that likely enable survival in dryland ecosystems.

Biological soil crusts

Estimating Sonic Boom Metrics Across a Community Using a Kalman Filter

As part of the Quesst mission, NASA will fly the X‑59 aircraft over selected communities to evaluate community responses to low-intensity sonic booms. The purpose of these community tests is to determine the dose-response relationship between the noise exposure metrics and the community response. The independent variables for the dose-response relationship are the noise exposure metrics experienced by survey respondents within the community. Two sources of noise exposure metrics are available in each community: measurements at sparse locations throughout the community, and calculations from propagation models across the community. Both the measurements and calculations are subject to uncertainty. A Kalman filter is proposed to combine the measured and calculated noise exposure metrics to obtain the best estimate of the true noise exposure metrics across the community. The noise exposure metrics estimated by the Kalman filter have lower uncertainty than either the measured or calculated noise exposure metrics alone. Simulations demonstrate that the Kalman filter produces a more accurate estimate of the true noise exposure metrics than other noise estimation methods.

Sonic boom

AIAA ASCEND 2023 Panel Session Proposal: Community Perspectives on Establishing a Government-Funded National Laboratory in a Commercially-Driven LEO Ecosystem

AIAA ASCEND 2023 Panel Session Proposal: Community Perspectives on Establishing a Government-Funded National Laboratory in a Commercially-Driven LEO Ecosystem Submitted by the NASA Office of Technology, Policy, and Strategy March 8, 2023 Session Format: Panel Session Topic: Expanding and Evolving the Space Economy Session Title: Community Perspectives on Establishing a Government-Funded National Laboratory in a Commercially-Driven LEO Ecosystem Short Session Description: NASA is preparing for the retirement of the ISS and transition of government-sponsored low-Earth orbit (LEO) activities to Commercial LEO Destinations (CLDs), which includes a government-funded national lab. This transition necessitates new models to connect government-funded users of the LEO environment to CLDs. This panel will highlight updates on what may constitute a national laboratory, share perspectives from members of LEO user communities, and open the discussion for additional comments. Requested Session Duration: 90 minutes Extended Session Description: NASA is preparing for the retirement of the ISS and transition of low-Earth orbit (LEO) activities to Commercial LEO Destinations (CLDs), including planning for a national laboratory. This transition necessitates new models for connecting government-funded users of the LEO environment to CLDs. NASA has made progress on models and a stepwise strategy to enable this national laboratory. This session will discuss guiding national strategies and highlight perspectives on what constitutes a national laboratory from current user communities. A NASA panel participant will introduce the rationale for continuing a national laboratory utilizing CLDs and speak on progress-to-date. This includes furthering national priorities to continue a space-based microgravity national laboratory. It also may include a brief overview of a study conducted by NASA which elucidates a trade space of models for a national laboratory and highlights from an interagency working group on microgravity for additional context. A moderator and five panelists representing diverse LEO user communities will then share perspectives on topics relating to this new dynamic ecosystem, expectations for models and activities, and other thoughts on NASA’s progress. Panelists may consist of members from the following communities: NASA, other U.S. government agencies, startups, small businesses, academia, and non-profits. The panel then plans to open discussion to the audience for questions, input, and additional feedback. Session Goal(s)/Outcome(s): By hosting a panel session, NASA aims to accomplish two goals. First, NASA wishes to update the community on progress towards establishing a national laboratory in low-Earth orbit. This includes sharing key perspectives heard from stakeholder communities. The second goal is to highlight the collaborative nature of such an undertaking and to facilitate additional discussion with other current and future users of the LEO community. Participants (not exhaustive): Erica Rodgers; Robyn Gatens

Erica Marie Rodgers Heusinkveld

Tracking Community Building in Open Science

Open Science is enabled by a vibrant community of researchers who regularly engage with the data, from its production to its organization, curation, archiving, dissemination, analysis, and publication. This presentation will examine community building in open science. The NASA Open Science Data Repository (OSDR) makes data available to the public following the FAIR (Findability, Accessibility, Interoperability, and Reusability) principles. OSDR takes open science further with the OS Analysis Working Groups (AWGs) that facilitate community development and promotion. The primary activity of each AWG is to establish and validate analytical processes to generate higher-order data from data housed in OSDR. There are a number of these groups on various topics, including the Animal AWG, Plant AWG, Microbial AWG, Multi-Omics AWG, AI/ML AWG, and the Ames Life Sciences Data Archive (ALSDA) AWG. The international volunteers participating in these AWGs come from academia, citizen science initiatives, industry, and government. They include researchers, principal investigators, professors, trained hobbyists, and students from various domains and disciplines. Anyone may request to join the AWGs, and membership requests are vetted monthly by the group organizers before granting admission. Core to membership is demonstrated expertise through records of training, integrity, work in the professed domain(s), and good community standing. Regular virtual meetings are held for each AWG, with a varying cadence depending on the group's needs and goals. AWG communities share their expertise in research including cutting edge tools, software, frameworks, data formats, and libraries accelerating research collectively. This collaborative approach helps community members cross technology gaps and identify emerging challenges. These diverse communities encompass a wide range of individuals hailing from various sectors within the Science Mission Directorate and beyond. They serve as a means to promote and enhance transparency, accessibility, and inclusion. An annual AWG Symposium brings contributors together in person. Participation in AWGs can be synchronous or asynchronous, with some groups performing most of their work in off hours. Participants gain valuable skills and connections that allow them to add value to their communities and new organizations that they join, resulting in an expanded return on investment for the space life science community. Open science is increasingly a federal mandate and initiatives like NASA's Transform to Open Science and instruments like the Decadal Survey of Biological and Physical Sciences in Space demonstrate the need to carefully consider best practices in this domain. Here, we present greater detail about the makeup and participation metrics of the various AWGs affiliated with OSDR and details of successful peer-reviewed publication campaigns.

Christina M Johnson

Tracking Community Building in Open Science

Open Science is enabled by a vibrant community of researchers who regularly engage with the data, from its production to its organization, curation, archiving, dissemination, analysis, and publication. This presentation will examine community building in open science. The NASA Open Science Data Repository (OSDR) makes data available to the public following the FAIR (Findability, Accessibility, Interoperability, and Reusability) principles. OSDR takes open science further with the OS Analysis Working Groups (AWGs) that facilitate community development and promotion. The primary activity of each AWG is to establish and validate analytical processes to generate higher-order data from data housed in OSDR. There are a number of these groups on various topics, including the Animal AWG, Plant AWG, Microbial AWG, Multi-Omics AWG, AI/ML AWG, and the Ames Life Sciences Data Archive (ALSDA) AWG. The international volunteers participating in these AWGs come from academia, citizen science initiatives, industry, and government. They include researchers, principal investigators, professors, trained hobbyists, and students from various domains and disciplines. Anyone may request to join the AWGs, and membership requests are vetted monthly by the group organizers before granting admission. Core to membership is demonstrated expertise through records of training, integrity, work in the professed domain(s), and good community standing. Regular virtual meetings are held for each AWG, with a varying cadence depending on the group's needs and goals. AWG communities share their expertise in research including cutting edge tools, software, frameworks, data formats, and libraries accelerating research collectively. This collaborative approach helps community members cross technology gaps and identify emerging challenges. These diverse communities encompass a wide range of individuals hailing from various sectors within the Science Mission Directorate and beyond. They serve as a means to promote and enhance transparency, accessibility, and inclusion. An annual AWG Symposium brings contributors together in person. Participation in AWGs can be synchronous or asynchronous, with some groups performing most of their work in off hours. Participants gain valuable skills and connections that allow them to add value to their communities and new organizations that they join, resulting in an expanded return on investment for the space life science community. Open science is increasingly a federal mandate and initiatives like NASA's Transform to Open Science and instruments like the Decadal Survey of Biological and Physical Sciences in Space demonstrate the need to carefully consider best practices in this domain. Here, we present greater detail about the makeup and participation metrics of the various AWGs affiliated with OSDR and details of successful peer-reviewed publication campaigns.

Christina M Johnson

Environmental Regulation of Microbial Community Structure

Most naturally occurring microbes live in complex microbial communities consisting of thousands of phylotypes of microorganisms living in close proximity. Each of these draws nutrients from the environment and releases metabolic waste products, which may in turn serve as substrates for other microbial groups. Gross environmental changes, such as irradiance level, hydrodynamic flow regime, temperature or water chemistry can directly affect the productivity of some community members, which in turn will affect other dependent microbial populations and rate processes. As a first step towards the development of "standard" natural communities of microorganisms for a variety of potential NASA applications, we are measuring biogeochemical cycling in artificially structured communities of microorganisms, created using natural microbial mat communities as inoculum. The responses of these artificially assembled communities of microorganisms to controlled shifts in ecosystem incubation conditions is being determined. This research requires close linking of environmental monitoring, with community composition in a closed and controlled incubation setting. We are developing new incubation chamber designs to allow for this integrated approach to examine the interplay between environmental conditions, microbial community composition and biogeochemical processes.

Bebout, Leslie

Composition and physiological profiling of sprout-associated microbial communities

The native microfloras of various types of sprouts (alfalfa, clover, sunflower, mung bean, and broccoli sprouts) were examined to assess the relative effects of sprout type and inoculum factors (i.e., sprout-growing facility, seed lot, and inoculation with sprout-derived inocula) on the microbial community structure of sprouts. Sprouts were sonicated for 7 min or hand shaken with glass beads for 2 min to recover native microfloras from the surface, and the resulting suspensions were diluted and plated. The culturable fraction was characterized by the density (log CFU/g), richness (e.g., number of types of bacteria), and diversity (e.g., microbial richness and evenness) of colonies on tryptic soy agar plates incubated for 48 h at 30 degrees C. The relative similarity between sprout-associated microbial communities was assessed with the use of community-level physiological profiles (CLPPs) based on patterns of utilization of 95 separate carbon sources. Aerobic plate counts of 7.96 +/- 0.91 log CFU/g of sprout tissue (fresh weight) were observed, with no statistically significant differences in microbial cell density, richness, or diversity due to sprout type, sprout-growing facility, or seed lot. CLPP analyses revealed that the microbial communities associated with alfalfa and clover sprouts are more similar than those associated with the other sprout types tested. Variability among sprout types was more extensive than any differences between microbial communities associated with alfalfa and clover sprouts from different sprout-growing facilities and seed lots. These results indicate that the subsequent testing of biocontrol agents should focus on similar organisms for alfalfa and clover, but alternative types may be most suitable for the other sprout types tested. The inoculation of alfalfa sprouts with communities derived from various sprout types had a significant, source-independent effect on microbial community structure, indicating that the process of inoculation alters the dynamics of community development regardless of the types of organisms involved.

NASA Center KSC