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At least 19 records

Block Design with Common Reference Samples Enables Robust Large-Scale Label-Free Quantitative Proteome Profiling

Label-free quantitative proteomics has become an increasingly popular tool for profiling global protein abundances. However, one major weakness is the potential performance drift of the LC-MS platform over time, which in turn limits its utility for analyzing large-scale sample sets. To address this, in this work we introduce an experimental and data analysis scheme based on a block-design with common controls within each block for enabling LC-MS-based large-scale label-free quantification. In this scheme, a large number of samples (e.g., >100 samples) are analyzed in smaller, and more manageable blocks, minimizing instrument drift and variability within a block. Furthermore, each designated block also contains common controls (or reference) samples for normalization within and across blocks. We demonstrated the effectiveness of this method by profiling the proteome response of human macrophage THP-1 cells to 11 engineered nanomaterials (ENMs) at two different doses. A total of 116 samples were analyzed in six blocks, yielding an average coverage of 4500 proteins per sample. The data revealed consistent quantification of proteins across all six blocks, as shown by highly stable quantification of house-keeping proteins in all samples and high levels of quantification correlation among samples from different blocks. The data also demonstrated that label-free quantification is robust and accurate enough to quantify even very subtle abundance changes as well as large fold-changes without potential ratio compression as often encountered with isobaric labeling. Our streamlined workflow is easy to implement and can be readily adapted to other large cohort studies for reproducible label-free proteome quantification.

59 BASIC BIOLOGICAL SCIENCES↗

The Radiometric Scaling of the MODIS and VIIRS Imagers to a Common Reference and Stability Analysis for the Next Edition of CERES Products

The NASA CERES project has provided the climate quality observed TOA and computed surface fluxes to the scientific community. CERES uses MODIS and VIIRS imagers to retrieve cloud properties needed to convert Terra, Aqua, NPP, and NOAA-20 CERES footprint radiance observations into fluxes. The imagers are also used to radiometrically scale the geostationary sensors (GEO) radiances to the imager calibration reference to ensure that the GEO derived cloud properties and broadband TOA fluxes are consistent in both space and time. Both the imager and GEO retrieved cloud properties are used to compute the surface fluxes. The CERES imager and GEO calibration team uses ray-matched radiance pairs to radiometrically scale the SNPP and NOAA-20 VIIRS sensors to the Aqua-MODIS calibration reference. The radiometric scaling is further validated using geostationary imagers as transfer radiometers. The team will rely primarily on deep convective clouds to monitor the imager channel calibration stability and to correct for short term calibration drifts. The DSCOVR satellite was launched on February 25, 2015 and orbits around the L1 Lagrange point directly between the Earth and the sun. The EPIC sensor contains no onboard calibration systems. However, multiple inter-calibration studies have shown that the EPIC imager is very stable in time. The excellent radiometric stability of EPIC allows the use of EPIC observations as a stable reference to validate both the short term drift corrections of the imagers, as well as to validate the radiometric scaling factors between them. Examples of the imager relative calibration using EPIC before and after radiometric scaling will be shown along with the results from the use of invariant targets to remove imager calibration drifts.

DSCOVR-EPIC↗

Compilation of Published Estimates of Annual Geocenter Motions Using Space Geodesy

The definition of the term "geocenter motion" depends on the adopted origin of the reference frame. Common reference frames used in Space Geodesy include: the center of mass of the whole Earth (CM), the center of mass of the Solid Earth without mass load (CE), and the center of figure of the outer surface of the Solid Earth (CF). There are two established definitions of the term geocenter: one, the vector offset of CF relative to CM and, two, the reverse, the vector offset of CM relative to CF. Obviously, their amplitude is the same and their phase differs by 180 deg. Following Dong et al. [2003], we label the first X(sub CF, sup CM) and the second X(sup CF, sup CM) (i.e., the superscript represents the frame, the subscript represents any point in the frame).

Elosegui, P.↗

Device and Method for Digital-To-Analog Transformations and Reconstructions of Multi-Channel Electrocardiograms

The present invention includes a method and apparatus for digital to analog conversion and reconstruction of multichannel electrocardiograms. The method may include receiving digital information representative of a plurality of independent signals, producing a plurality of analog outputs from said digital information wherein a first analog output is designated as a common reference, and imposing a predetermined voltage on a second analog output with respect to said common reference, which provides for a substantial recreation of the original independent signals. The apparatus may comprise a processor operable for receiving digital information representative of independent lead signals from a first ECG machine and digital to analog circuitry for substantially reproducing the original lead signals for analysis on a second ECG machine for convenient and efficient second opinions of cardiac data.

Schlegel, Todd T.↗

Consequences of plane-strain and plane-stress assumptions in fully coupled chemo-mechanical Li-ion battery models

In Li-ion battery research, it is common to simulate chemo-mechanical phenomena in reduced dimensions (e.g., 2-D) as opposed to fully resolve these complex physics in 3-D. It is common to assume either (1) the out-of-plane strain is negligible (commonly referred to as plane-strain), or (2) the out-of-plane stress is negligible (commonly referred to as plane-stress). However, there is typically little consideration as to the quantitative consequences of these approximations. Furthermore, the influence of these out-of-plane assumptions can be compounded and convoluted when chemo-mechanics models implement so-called “fully coupled” formulations, where the local species concentrations influence the stress-state and the stress-state influences the local species fluxes. Here, the present manuscript explores the implications of using plane-stress and plane-strain assumptions in 2-D as compared to simulating a full 3-D electrode particle. This comparative study includes simulating both isotropic and anisotropic particle intercalation where the particles can be surrounded by either a liquid or solid electrolyte. Additionally, common Li-ion battery-model metrics such as the state-of-stress, intercalation fraction distribution, and specific capacity are compared, while also considering the effects of particle size and C-rate. As alternatives to the pure plane-strain and plane stress approximations, two modified plane-strain assumptions are found to better approximate the fully coupled chemo-mechanical 3-D behavior.

25 ENERGY STORAGE↗

TMD Handbook

This handbook provides a comprehensive review of transverse-momentum-dependent parton distribution functions and fragmentation functions, commonly referred to as transverse momentum distributions (TMDs). TMDs describe the distribution of partons inside the proton and other hadrons with respect to both their longitudinal and transverse momenta. They provide unique insight into the internal momentum and spin structure of hadrons, and are a key ingredient in the description of many collider physics cross sections. Understanding TMDs requires a combination of theoretical techniques from quantum field theory, nonperturbative calculations using lattice QCD, and phenomenological analysis of experimental data. The handbook covers a wide range of topics, from theoretical foundations to experimental analyses, as well as recent developments and future directions. It is intended to provide an essential reference for researchers and graduate students interested in understanding the structure of hadrons and the dynamics of partons in high energy collisions.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Work Breakdown Structure (WBS) Handbook

The purpose of this document is to provide program/project teams necessary instruction and guidance in the best practices for Work Breakdown Structure (WBS) and WBS dictionary development and use for project implementation and management control. This handbook can be used for all types of NASA projects and work activities including research, development, construction, test and evaluation, and operations. The products of these work efforts may be hardware, software, data, or service elements (alone or in combination). The aim of this document is to assist project teams in the development of effective work breakdown structures that provide a framework of common reference for all project elements. The WBS and WBS dictionary are effective management processes for planning, organizing, and administering NASA programs and projects. The guidance contained in this document is applicable to both in-house, NASA-led effort and contracted effort. It assists management teams from both entities in fulfilling necessary responsibilities for successful accomplishment of project cost, schedule, and technical goals. Benefits resulting from the use of an effective WBS include, but are not limited to: providing a basis for assigned project responsibilities, providing a basis for project schedule development, simplifying a project by dividing the total work scope into manageable units, and providing a common reference for all project communication.

Source record↗

NASA Work Breakdown Structure (WBS) Handbook

The purpose of this document is to provide program/project teams necessary instruction and guidance in the best practices for Work Breakdown Structure (WBS) and WBS dictionary development and use for project implementation and management control. This handbook can be used for all types of NASA projects and work activities including research, development, construction, test and evaluation, and operations. The products of these work efforts may be hardware, software, data, or service elements (alone or in combination). The aim of this document is to assist project teams in the development of effective work breakdown structures that provide a framework of common reference for all project elements. The WBS and WBS dictionary are effective management processes for planning, organizing, and administering NASA programs and projects. The guidance contained in this document is applicable to both in-house, NASA-led effort and contracted effort. It assists management teams from both entities in fulfilling necessary responsibilities for successful accomplishment of project cost, schedule, and technical goals. Benefits resulting from the use of an effective WBS include, but are not limited to: providing a basis for assigned project responsibilities, providing a basis for project schedule and budget development, simplifying a project by dividing the total work scope into manageable units, and providing a common reference for all project communication.

Planning↗

NASA Work Breakdown Structure (WBS) Handbook

The purpose of this document is to provide program/project teams necessary instruction and guidance in the best practices for WBS and WBS dictionary development and use for project implementation and management control. This handbook can be used for all types of NASA projects and work activities including research, development, construction, test and evaluation, and operations. The products of these work efforts may be hardware, software, data, or service elements (alone or in combination). The aim of this document is to assist project teams in the development of effective work breakdown structures that provide a framework of common reference for all project elements. The WBS and WBS dictionary are effective management processes for planning, organizing, and administering NASA programs and projects. The guidance contained in this document is applicable to both in-house, NASA-led effort and contracted effort. It assists management teams from both entities in fulfilling necessary responsibilities for successful accomplishment of project cost, schedule, and technical goals. Benefits resulting from the use of an effective WBS include, but are not limited to: providing a basis for assigned project responsibilities, providing a basis for project schedule and budget development, simplifying a project by dividing the total work scope into manageable units, and providing a common reference for all project communication.

Christopher Lewis Sadler↗

Resource-Efficient Chemistry on Quantum Computers with the Variational Quantum Eigensolver and The Double Unitary Coupled-Cluster approach

Applications of quantum simulation algorithms to obtain electronic energies of molecules on noisy intermediate-scale quantum (NISQ) devices require careful consideration of resources describing the complex electron correlation effects. In modeling second-quantized problems, the biggest challenge confronted is that the number of qubits scales linearly with the size of molecular basis. This poses a significant limitation on the size of the basis sets and the number of correlated electrons included in quantum simulations of chemical processes. To address this issue and to enable more realistic simulations on NISQ computers, we employ the double unitary coupled-cluster (DUCC) method to effectively downfold correlation effects into the reduced-size orbital space, commonly referred to as the active space. Using downfolding techniques, we demonstrate that properly constructed effective Hamiltonians can capture the effect of the whole orbital space in small-size active spaces. Combining the downfolding pre-processing technique with the Variational Quantum Eigensolver, we solve for the ground-state energy of H2 and Li2 in the cc-pVTZ basis using the DUCC-reduced active spaces. We compare these results to full configuration-interaction and high-level coupled-cluster reference calculations.

quantum computing, variational quantum solver, cou↗

The Angstrom Exponent and Bimodal Aerosol Size Distributions

Powerlaws have long been used to describe the spectral dependence of aerosol extinction, and the wavelength exponent of the aerosol extinction powerlaw is commonly referred to as the Angstrom exponent. The Angstrom exponent is often used as a qualitative indicator of aerosol particle size, with values greater than two indicating small particles associated with combustion byproducts, and values less than one indicating large particles like sea salt and dust. In this study, we investigate the relationship between the Angstrom exponent and the mode parameters of bimodal aerosol size distributions using Mie theory calculations and Aerosol Robotic Network (AERONET) retrievals. We find that Angstrom exponents based upon seven wavelengths (0.34, 0.38, 0.44, 0.5, 0.67, 0.87, and 1.02 micrometers) are sensitive to the volume fraction of aerosols with radii less then 0.6 micrometers, but not to the fine mode effective radius. The Angstrom exponent is also known to vary with wavelength, which is commonly referred to as curvature; we show how the spectral curvature can provide additional information about aerosol size distributions for intermediate values of the Angstrom exponent. Curvature also has a significant effect on the conclusions that can be drawn about two-wavelength Angstrom exponents; long wavelengths (0.67, 0.87 micrometers) are sensitive to fine mode volume fraction of aerosols but not fine mode effective radius, while short wavelengths (0.38, 0.44 micrometers) are sensitive to the fine mode effective radius but not the fine mode volume fraction.

Schuster, Gregory L.↗

PHIL Interface Design for Use With a Voltage-Regulated Amplifier

Power hardware-in-the-loop (PHIL) has emerged as a leading strategy to thoroughly assess the impact of proprietary inverter controls on a specific power system. The development of a PHIL test bed typically involves an inverter under test, a power amplifier, controllable DC supply, and a digital real-time simulator (DRTS) to simulate the power system under study. As a result of PHIL nonidealities, a form of digital compensation within the DRTS is used, which is commonly referred to as a PHIL interface. Many existing methods use legacy power amplifiers that do not contain internal voltage regulation. These existing interface methods are based around a voltage regulator within the DRTS and do not consider the interaction with the controls in newer amplifiers. In this study, a three-step approach of PHIL interface development for modern power amplifiers with built-in voltage regulation is introduced and is validated in hardware with a 30-kW grid-following inverter.

DRTS↗

Design and Development of the SMAP Microwave Radiometer Electronics

The SMAP microwave radiometer will measure land surface brightness temperature at L-band (1413 MHz) in the presence of radio frequency interference (RFI) for soil moisture remote sensing. The radiometer design was driven by the requirements to incorporate internal calibration, to operate synchronously with the SMAP radar, and to mitigate the deleterious effects of RFI. The system design includes a highly linear super-heterodyne microwave receiver with internal reference loads and noise sources for calibration and an innovative digital signal processor and detection system. The front-end comprises a coaxial cable-based feed network, with a pair of diplexers and a coupled noise source, and radiometer front-end (RFE) box. Internal calibration is provided by reference switches and a common noise source inside the RFE. The RF back-end (RBE) downconverts the 1413 MHz channel to an intermediate frequency (IF) of 120 MHz. The IF signals are then sampled and quantized by high-speed analog-to-digital converters in the radiometer digital electronics (RDE) box. The RBE local oscillator and RDE sampling clocks are phase-locked to a common reference to ensure coherency between the signals. The RDE performs additional filtering, sub-band channelization, cross-correlation for measuring third and fourth Stokes parameters, and detection and integration of the first four raw moments of the signals. These data are packetized and sent to the ground for calibration and further processing. Here we discuss the novel features of the radiometer hardware particularly those influenced by the need to mitigate RFI.

radio frequency interference↗

An International Strategy for Human Exploration of the Moon: The International Space Exploration Coordination Group (ISECG) Reference Architecture for Human Lunar Exploration

The International Space Exploration Coordination Group (ISECG) was established in response to The Global Exploration Strategy: The Framework for Coordination developed by fourteen space agencies and released in May 2007. Several ISECG participating space agencies have been studying concepts for human exploration of the moon that allow individual and collective goals and objectives to be met. This 18 month study activity culminated with the development of the ISECG Reference Architecture for Human Lunar Exploration. The reference architecture is a series of elements delivered over time in a flexible and evolvable campaign. This paper will describe the reference architecture and how it will inform near-term and long-term programmatic planning within interested agencies. The reference architecture is intended to serve as a global point of departure conceptual architecture that enables individual agency investments in technology development and demonstration, International Space Station research and technology demonstration, terrestrial analog studies, and robotic precursor missions to contribute towards the eventual implementation of a human lunar exploration scenario which reflects the concepts and priorities established to date. It also serves to create opportunities for partnerships that will support evolution of this concept and its eventual realization. The ISECG Reference Architecture for Human Lunar Exploration (commonly referred to as the lunar gPoD) reflects the agency commitments to finding an effective balance between conducting important scientific investigations of and from the moon, as well as demonstrating and mastering the technologies and capabilities to send humans farther into the Solar System. The lunar gPoD begins with a robust robotic precursor phase that demonstrates technologies and capabilities considered important for the success of the campaign. Robotic missions will inform the human missions and buy down risks. Human exploration will start with a thorough scientific investigation of the polar region while allowing the ability to demonstrate and validate the systems needed to take humans on more ambitious lunar exploration excursions. The ISECG Reference Architecture for Human Lunar Exploration serves as a model for future cooperation and is documented in a summary report and a comprehensive document that also describes the collaborative international process that led to its development. ISECG plans to continue with architecture studies such as this to examine an open transportation architecture and other destinations, with expanded participation from ISECG agencies, as it works to inform international partnerships and advance the Global Exploration Strategy.

Laurini, Kathleen C.↗

Space shuttle: A comparison of wind tunnel facility data on the Grumman H-33 delta winged orbiter

A comparison of longitudinal and lateral directional stability and control characteristics of the Phase B Grumman H-33 orbiter configuration obtained from 6 tests conducted in 4 different facilities is presented. The H-33 orbiter configuration does not include drop tanks. Slope data are used as the medium for comparison. The slope data were computed using the SADSAC system and have been reduced to a common reference system. Data used for comparison were selected based on common configurations wherever possible. Where direct configuration comparisons were not possible, the parameters selected for comparison are regarded as insensitive to the noted differences. Factors considered in comparing the various data were scaling effects, balance operating ranges, balance sensitivities and control deflection accuracies. All factors considered, the comparison among data obtained from the various facilities is excellent. The minor differences in stability and control characteristics are well within the accuracy band of preliminary design model construction specifications.

Gyorfi, R. A.↗

Quantitative Fit Evaluation of N95 Filtering Facepiece Respirators and Coronavirus Inactivation Following Heat Treatment

Abstract Reuse of filtering facepiece respirators (FFRs, commonly referred to as N95s) normally meant for single use has become common in healthcare facilities due to shortages caused by the COVID-19 pandemic. Here, we report that murine hepatitis coronavirus initially seeded on FFR filter material is inactivated (6 order of magnitude reduction as measured by median tissue culture infective dose, TCID50) after dry heating at 75°C for 30 min. We also find that the quantitative fit of FFRs after heat treatment at this temperature, under dry conditions or at 90% relative humidity, is not affected by single or 10 heating cycles. Previous studies have reported that the filtration efficiency of FFRs is not negatively impacted by these heating conditions. These results suggest that thermal inactivation of coronaviruses is a potentially rapid and widely deployable method to reuse N95 FFRs in emergency situations where reusing FFRs is a necessity and broad-spectrum sterilization is unavailable. However, we also observe that a radiative heat source (e.g. an exposed heating element) results in rapid qualitative degradation of the FFR. Finally, we discuss differences in the results reported here and other recent studies investigating heat as a means to recycle FFRs. These differences suggest that while our repeated decontamination cycles do not affect FFR fit, overall wear time and the number of donning/doffing cycles are important factors that likely degrade FFR fit and must be investigated further.

60 APPLIED LIFE SCIENCES↗

A Transportable Gravity Gradiometer Based on Atom Interferometry

A transportable atom interferometer-based gravity gradiometer has been developed at JPL to carry out measurements of Earth's gravity field at ever finer spatial resolutions, and to facilitate high-resolution monitoring of temporal variations in the gravity field from ground- and flight-based platforms. Existing satellite-based gravity missions such as CHAMP and GRACE measure the gravity field via precise monitoring of the motion of the satellites; i.e. the satellites themselves function as test masses. JPL's quantum gravity gradiometer employs a quantum phase measurement technique, similar to that employed in atomic clocks, made possible by recent advances in laser cooling and manipulation of atoms. This measurement technique is based on atomwave interferometry, and individual laser-cooled atoms are used as drag-free test masses. The quantum gravity gradiometer employs two identical atom interferometers as precision accelerometers to measure the difference in gravitational acceleration between two points (Figure 1). By using the same lasers for the manipulation of atoms in both interferometers, the accelerometers have a common reference frame and non-inertial accelerations are effectively rejected as common mode noise in the differential measurement of the gravity gradient. As a result, the dual atom interferometer-based gravity gradiometer allows gravity measurements on a moving platform, while achieving the same long-term stability of the best atomic clocks. In the laboratory-based prototype (Figure 2), the cesium atoms used in each atom interferometer are initially collected and cooled in two separate magneto-optic traps (MOTs). Each MOT, consisting of three orthogonal pairs of counter-propagating laser beams centered on a quadrupole magnetic field, collects up to 10(exp 9) atoms. These atoms are then launched vertically as in an atom fountain by switching off the magnetic field and introducing a slight frequency shift between pairs of lasers to create a moving rest frame for the trapped atoms. While still in this moving-frame molasses, the laser frequencies are further detuned from the atomic resonance (while maintaining this relative frequency shift) to cool the atom cloud's temperature to 2 K or below, corresponding to an rms velocity of less than 2 cm/s. After launch, the cold atoms undergo further state and velocity selection to prepare for atom interferometry. The atom interferometers are then realized using laser-induced stimulated Raman transitions to perform the necessary manipulations of each atom, and the resulting interferometer phase is measured using laser-induced fluorescence for state-normalized detection. More than 20 laser beams with independent controls of frequency, phase, and intensity are required for this measurement sequence. This instrument can facilitate the study of Earth's gravitational field from surface and air vehicles, as well as from space by allowing gravity mapping from a low-cost, single spacecraft mission. In addition, the operation of atom interferometer-based instruments in space offers greater sensitivity than is possible in terrestrial instruments due to the much longer interrogation times available in the microgravity environment. A space-based quantum gravity gradiometer has the potential to achieve sensitivities similar to the GRACE mission at long spatial wavelengths, and will also have resolution similar to GOCE for measurement at shorter length scales.

Yu, Nan↗

Quantitative form and fit of N95 filtering facepiece respirators are retained after dry and humid heat treatments for coronavirus deactivation

Re-use of filtering facepiece respirators (FFRs, commonly referred to as N95s) normally meant for single use only is becoming common in healthcare facilities due to shortages caused by the COVID19 pandemic. Here we report that mouse hepatitis virus (MHV) initially seeded on FFR filter material is inactivated (6 log reduction as measured by 50% tissue culture infective dose (TCID50)) after dry heating at 75 °C for 30 minutes. We also find that the quantitative fit of FFRs after heat treatment at this temperature, under dry conditions or at 90% relative humidity, is not affected by single or ten heating cycles. Previous studies have reported that the filtration efficiency of FFR filters is not negatively impacted by these heating conditions. These results suggest that thermal inactivation of coronaviruses is a potentially rapid and widely deployable method to re-use N95 FFRs in emergency situations where reusing FFRs is a necessity and broad-spectrum sterilization is unavailable. However, we also found that a heat source that emits radiation (e.g., an exposed heating element) results in rapid qualitative degradation of the FFR. Finally, we discuss differences in the results reported here and other recent studies investing heat as a means to recycle FFRs and suggest that overall wear time and donning/doffing cycles are important factors that need to be considered.

36 MATERIALS SCIENCE↗