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At least 19 records

Engineered enzymatic cascade converts diols to amino alcohols

Aliphatic amino alcohols such as 6-amino-1-hexanol are potential platform chemicals for a variety of advanced materials, but applications are currently limited by reagent costs. Aliphatic amino alcohols can currently be synthesized from biomass-derived diols at elevated temperatures and pressures using Ru-based catalysts that produce a mixture of amino-alcohol, diamine, and cyclic amine products. Replacing chemical amination with an enzymatic cascade would reduce resource needs and enable reactions under milder conditions. In this work, we characterized a two-enzyme cascade that selectively converts C4–C7 diols to the corresponding amino alcohols under aqueous conditions at room temperature and pressure. By engineering the rate-limiting enzyme and optimizing reaction conditions, we increased amino alcohol production nearly 30-fold, achieving a selectivity of 99%. The same enzyme cascade could also be used to convert amino alcohols into cyclic amines through reduction of the corresponding cyclic imine. This engineered cascade provides a green opportunity to sustainably synthesize asymmetric bifunctional platform chemicals.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH

A cascaded Schwarz converter for high frequency power distribution

It is shown that two Schwarz converters in cascade provide a very reliable 20-kHz source that features zero current commutation, constant frequency, and fault-tolerant operation, meeting requirements for spacecraft applications. A steady-state analysis of the converter is presented, and equations for the steady-state performance are derived. Fault-current limiting is discussed. Experimental results are presented for a 900-W version, which has been successfully tested under no-load, full-load, and short-circut conditions.

Ray, Biswajit

A 2.5 kW cascaded Schwarz converter for 20 kHz power distribution

Because it avoids the high currents in a parallel loaded capacitor, the cascaded Schwarz converter should offer better component utilization than converters with sinusoidal output voltages. The circuit is relatively easy to protect, and it provides a predictable trapezoidal voltage waveform that should be satisfactory for 20-kHz distribution systems. Analysis of the system is enhanced by plotting curves of normalized variables vs. gamma(1), where gamma(1) is proportional to the variable frequency of the first stage. Light-load operation is greatly improved by the addition of a power recycling rectifier bridge that is back biased at medium to heavy loads. Operation has been verified on a 2.5-kW circuit that uses input and output voltages in the same range as those anticipated for certain future spacecraft power systems.

Shetler, Russell E.

Cascaded resonant bridge converters

A converter for converting a low voltage direct current power source to a higher voltage, high frequency alternating current output for use in an electrical system where it is desired to use low weight cables and other circuit elements. The converter has a first stage series resonant (Schwarz) converter which converts the direct current power source to an alternating current by means of switching elements that are operated by a variable frequency voltage regulator, a transformer to step up the voltage of the alternating current, and a rectifier bridge to convert the alternating current to a direct current first stage output. The converter further has a second stage series resonant (Schwarz) converter which is connected in series to the first stage converter to receive its direct current output and convert it to a second stage high frequency alternating current output by means of switching elements that are operated by a fixed frequency oscillator. The voltage of the second stage output is controlled at a relatively constant value by controlling the first stage output voltage, which is accomplished by controlling the frequency of the first stage variable frequency voltage controller in response to second stage voltage. Fault tolerance in the event of a load short circuit is provided by making the operation of the first stage variable frequency voltage controller responsive to first and second stage current limiting devices. The second stage output is connected to a rectifier bridge whose output is connected to the input of the second stage to provide good regulation of output voltage wave form at low system loads.

Stuart, Thomas A.

A study of Schwarz converters for nuclear powered spacecraft

High power space systems which use low dc voltage, high current sources such as thermoelectric generators, will most likely require high voltage conversion for transmission purposes. This study considers the use of the Schwarz resonant converter for use as the basic building block to accomplish this low-to-high voltage conversion for either a dc or an ac spacecraft bus. The Schwarz converter has the important assets of both inherent fault tolerance and resonant operation; parallel operation in modular form is possible. A regulated dc spacecraft bus requires only a single stage converter while a constant frequency ac bus requires a cascaded Schwarz converter configuration. If the power system requires constant output power from the dc generator, then a second converter is required to route unneeded power to a ballast load.

Stuart, Thomas A.

A study of Schwarz converters for nuclear powered spacecraft

High power space systems which use low dc voltage, high current sources such as thermoelectric generators, will most likely require high voltage conversion for transmission purposes. This study considers the use of the Schwarz resonant converter for use as the basic building block to accomplish this low-to-high voltage conversion for either a dc or an ac spacecraft bus. The Schwarz converter has the important assets of both inherent fault tolerance and resonant operation; parallel operation in modular form is possible. A regulated dc spacecraft bus requires only a single stage converter while a constant frequency ac bus requires a cascaded Schwarz converter configuration. If the power system requires constant output power from the dc generator, then a second converter is required to route unneeded power to a ballast load.

Stuart, Thomas A.

CAS22 - FORTRAN program for fast design and analysis of shock-free airfoil cascades using fictitious-gas concept

A user-oriented computer program, CAS22, was developed that is applicable to aerodynamic analysis and transonic shock-free redesign of existing two-dimensional cascades of airfoils. This FORTRAN program can be used: (1) as an analysis code for full-potential, transonic, shocked or shock-free cascade flows; (2) as a design code for shock-free cascades that uses Sobieczky's fictitious-gas concept; and (3) as a shock-free design code followed automatically by the analysis in order to confirm that the newly obtained cascade shape provides for an entirely shock-free transonic flow field. A four-level boundary-conforming grid of an O type is generated. The shock-free design is performed by implementing Sobieczky's fictitious-gas concept of elliptic continuation from subsonic into supersonic flow domains. Recomputation inside each supersonic zone is performed by the method of characteristics in the rheograph plane by using isentropic gas relations. Besides converting existing cascade shapes with multiple shocked supersonic regions into shock-free cascades, CAS22 can also unchoke previously choked cascades and make them shock free.

Dulikravich, D. S.

Spectral energetics of the observed and simulated Northern Hemisphere general circulation during blocking episodes

The spectral energetics of the Northern Hemisphere circulation during blocking episodes of the FGGE year is investigated with gridded analyses of observational data and parallel simulation experiments. The purpose of this study is to describe the energetics distinctions of the observed and simulated blockings in the context of the general circulation and to assess the capability of the model to simulate blockings. In the observed circulation a pronounced winter blocking is developed and maintained by the nonlinear wave-wave interaction L(1) from the kinetic energy source for n = 3-10, where L(n) is the transfer of eddy kinetic energy from all other wavenumbers to wavenumber n. In the case of the double blocking in the winter, both L(1) and L(2) support the blocking. The kinetic energy source of n = 10 for upscale input at n = 1 and 2 is supported by the baroclinic conversion at n = 3-10. The simulated winter circulation shows strong baroclinic conversion at all wavenumbers, including ultralong waves. However, the simulation fails to produce pronounced blocking for the absence of L(1), and the converted energy cascades down to shorter waves. The wave-mean transfer of kinetic energy from the large-scale disturbances to the zonal mean component further prevents the accumulation of the kinetic energy at the ultralong waves. In contrast to the winter situation, the summer blocking seems to be directly supported by both L(4) and baroclinic conversion at other planetary-scale waves. Consequently, the summer circulation is better simulated than the winter circulation.

Kung, Ernest C.

Shockless design and analysis of transonic blade shapes

A fast computer program was developed to eliminate the shocks by slightly altering portions of the contour of a given airfoil in the cascade. The program can be used in two basic modes: (1) An analysis for steady, transonic, potential flow through a given planar cascade of airfoils and (2) a design for converting a given cascade into a shockless transonic cascade. The design mode can automatically be followed by the analysis mode, which confirms that the flow field is shock free. The program generates its own multilevel boundary conforming computational grids and solves a full potential equation in a fully conservative form. The shockless design is performed by implementing Sobieczky's fictitious-gas elliptic continuation concept.

Dulikravich, D. S.

Phenomenological model of nuclear primary air showers

The development of proton primary air showers is described in terms of a model based on a hadron core plus an electromagnetic cascade. The muon component is neglected. The model uses three parameters: a rate at which hadron core energy is converted into electromagnetic cascade energy and a two-parameter sea-level shower-age function. By assuming an interaction length for the primary nucleus, the model is extended to nuclear primaries. Both models are applied over the energy range from 10 to the 13th power to 10 to the 21st power eV. Both models describe the size and age structure (neglecting muons) from a depth of 342 to 2052 g/sq cm.

Tompkins, D. R., Jr.

Submodule-Level Decentralized Control for Cascaded Quadruple Active Bridge-Based Medium-Voltage Converters

High-power, low-voltage DC (LVDC) loads, such as AI data centers, electric vehicle fast-charging hubs, and hydrogen electrolyzers, are accelerating the deployment of solid-state transformers (SSTs) that directly interface medium-voltage AC (MVAC) to LVDC, streamlining power delivery. This paper proposes a submodule-level decentralized control strategy for an SST comprising a cascaded H-bridge (CHB) active rectifier on the MVAC side and per-module quadruple-active-bridge (QAB) converters on the LVDC side with local controllers. The proposed strategy eliminates the need for high-fidelity, real-time cross-isolation communication by synchronizing all active bridges to a common heartbeat signal and enabling the primary- and secondary-side controllers to generate independent local control signals (phase shifts). The resultant phase shifts naturally govern average and double-line frequency power transfer between active bridges, enabling each side to meet its control objectives using only local measurements. Detailed controller design considerations are presented based on a stability analysis. Comprehensive electromagnetic-transient simulations demonstrate tight DC-link voltage regulation, high-quality grid currents, and robust operation under load transients and grid disturbances, without real-time communication among the submodule controllers. These results indicate that the proposed scheme can significantly enhance the reliability, scalability, and flexibility of cascaded bridge-based medium-voltage converters.

24 POWER TRANSMISSION AND DISTRIBUTION

Submodule-Level Decentralized Control for Cascaded Quadruple Active Bridge-Based Medium-Voltage Converters: Preprint

High-power, low-voltage DC (LVDC) loads, such as AI data centers, electric vehicle fast-charging hubs, and hydrogen electrolyzers, are accelerating the deployment of solid-state transformers (SSTs) that directly interface medium-voltage AC (MVAC) to LVDC, streamlining power delivery. This paper proposes a submodule-level decentralized control strategy for an SST comprising a cascaded H-bridge (CHB) active rectifier on the MVAC side and per-module quadruple-active-bridge (QAB) converters on the LVDC side with local controllers. The proposed strategy eliminates the need for high-fidelity, real-time cross-isolation communication by synchronizing all active bridges to a common heartbeat signal and enabling the primary- and secondary-side controllers to generate independent local control signals (phase shifts). The resultant phase shifts naturally govern average and double-line frequency power transfer between active bridges, enabling each side to meet its control objectives using only local measurements. Detailed controller design considerations are presented based on a stability analysis. Comprehensive electromagnetic-transient simulations demonstrate tight DC-link voltage regulation, high-quality grid currents, and robust operation under load transients and grid disturbances, without real-time communication among the submodule controllers. These results indicate that the proposed scheme can significantly enhance the reliability, scalability, and flexibility of cascaded bridge-based medium-voltage converters.

24 POWER TRANSMISSION AND DISTRIBUTION

Cell-Free Systems Biology: Characterizing Central Metabolism of Clostridium thermocellum with a Three-Enzyme Cascade Reaction

Genetic approaches have been traditionally used to understand microbial metabolism, but this process can be slow in nonmodel organisms due to limited genetic tools. An alternative approach is to study metabolism directly in the cell lysate. This avoids the need for genetic tools and is routinely used to study individual enzymatic reactions but is not generally used to study systems-level properties of metabolism. Here we demonstrate a new approach that we call “cell-free systems biology”, where we use well-characterized enzymes and multienzyme cascades to serve as sources or sinks of intermediate metabolites. This allows us to isolate subnetworks within metabolism and study their systems-level properties. To demonstrate this, we worked with a threeenzyme cascade reaction that converts pyruvate to 2,3-butanediol. Although it has been previously used in cell-free systems, its pH dependence was not well characterized, limiting its utility as a sink for pyruvate. We showed that improved proton accounting allowed better prediction of pH changes and that active pH control allowed 2,3-butanediol titers of up to 2.1 M (189 g/L) from acetoin and 1.6 M (144 g/L) from pyruvate. The improved proton accounting provided a crucial insight that preventing the escape of CO 2 from the system largely eliminated the need for active pH control, dramatically simplifying our experimental setup. We then used this cascade reaction to understand limits to product formation in Clostridium thermocellum, an organism with potential applications for cellulosic biofuel production. We showed that the fate of pyruvate is largely controlled by electron availability and that reactions upstream of pyruvate limit overall product formation.

09 BIOMASS FUELS

Optical Sidebands Multiplier

Optical sidebands have been generated with relative frequency tens to hundreds of GHz by using optical sidebands that are generated in a cascade process in high-quality optical resonators with Kerr nonlinearity, such as whispering gallery mode (WGM) resonators. For this purpose, the WGM resonator needs to be optically pumped at two frequencies matching its resonances. These two optical components can be one or several free spectral ranges (FSRs), equal to approximately 12 GHz, in this example, apart from each other, and can be easily derived from a monochromatic pump with an ordinary EOM (electro-optic modulation) operating at half the FSR frequency. With sufficient nonlinearity, an optical cascade process will convert the two pump frequencies into a comb-like structure extending many FSRs around the carrier frequency. This has a demonstratively efficient frequency conversion of this type with only a few milliwatt optical pump power. The concept of using Kerr nonlinearity in a resonator for non-degenerate wave mixing has been discussed before, but it was a common belief that this was a weak process requiring very high peak powers to be observable. It was not thought possible for this approach to compete with electro-optical modulators in CW applications, especially those at lower optical powers. By using the high-Q WGM resonators, the effective Kerr nonlinearity can be made so high that, using even weak seeding bands available from a conventional EOM, one can effectively multiply the optical sidebands, extending them into an otherwise inaccessible frequency range.

Strekalov, Dmitry V.

Per-Phase Control for CHB Converters with Negative-Sequence Current and DC Voltage Balancing Control

This paper presents a per-phase controller for a grid-connected Cascaded H-Bridge (CHB) converter featuring negative sequence current regulation and DC bus voltage balancing capabilities. Unlike conventional three-phase controllers, each phase of the CHB can be separately and controlled to regulate the DC voltage and reactive power flow. per-phase negative sequence current control strategy is incorporated into the proposed controller to ensure compliance with IEEE Standard 2800-2022, which requires the absorption of negative-sequence reactive current during grid voltage disturbances. Furthermore, a feed-forward control-based DC bus voltage balancing method is employed, which eliminates the need for the integrator commonly used in traditional approaches. The performance of the proposed controller is validated under various unbalanced grid scenarios and DC bus voltage imbalances through electromagnetic transient (EMT) simulation using a three-phase, three-level grid-connected CHB–Dual Active Bridge (DAB) converter testbed developed on MATLAB/Simulink. Additionally, the performance of the negative-sequence current regulation is investigated through simulation under different unbalanced grid voltage scenarios.

negative sequence current

Modelling, analyses and design of switching converters

A state-space averaging method for modelling switching dc-to-dc converters for both continuous and discontinuous conduction mode is developed. In each case the starting point is the unified state-space representation, and the end result is a complete linear circuit model, for each conduction mode, which correctly represents all essential features, namely, the input, output, and transfer properties (static dc as well as dynamic ac small-signal). While the method is generally applicable to any switching converter, it is extensively illustrated for the three common power stages (buck, boost, and buck-boost). The results for these converters are then easily tabulated owing to the fixed equivalent circuit topology of their canonical circuit model. The insights that emerge from the general state-space modelling approach lead to the design of new converter topologies through the study of generic properties of the cascade connection of basic buck and boost converters.

Cuk, S. M.