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At least 19 records

Detection of Chymotrypsin by Optical and Acoustic Methods

Chymotrypsin is an important proteolytic enzyme in the human digestive system that cleaves milk proteins through the hydrolysis reaction, making it an interesting subject to study the activity of milk proteases. In this work, we compared detection of chymotrypsin by spectrophotometric dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods and determined the limit of chymotrypsin detection (LOD), 0.15 ± 0.01 nM for spectrophotometric, 0.67 ± 0.05 nM for DLS and 1.40 ± 0.30 nM for QCM methods, respectively. The sensors are relatively cheap and are able to detect chymotrypsin in 3035 min. While the optical detection methods are simple to implement, the QCM method is more robust for sample preparation, and allows detection of chymotrypsin in non-transparent samples. We give an overview on methods and instruments for detection of chymotrypsin and other milk proteases.

59 BASIC BIOLOGICAL SCIENCES↗

Wake-Resolving Acoustic Tomography: Advances through Numerical Covariance Methods

Acoustic tomography offers path-integrated measurements of atmospheric velocity and temperature fluctuations with high spatial resolution. Classical implementations of time-dependent stochastic inversion rely on homogeneous, isotropic covariance models that are poorly suited to the anisotropic structure of wind turbine wakes. By directly estimating heterogeneous covariances from large-eddy simulations (LESs) into the time-dependent stochastic inversion operator, we relax implicit assumptions in the analytical models used historically. Retrievals using these LES-informed models improve agreement with true fields in variance, turbulent kinetic energy, and spectral content compared to analytical and precursor-based covariance models. The results indicate that LES-informed covariance models can enhance the accuracy of acoustic tomography retrievals in complex, anisotropic flows such as wind turbine wakes in some cases and highlight instances where analytical models still offer competitive performance, despite their simplifying assumptions.

17 WIND ENERGY↗

Nonlinear Impact-Echo Test for Quantitative Evaluation of ASR Damage in Concrete

Nonlinear acoustic methods demonstrate high sensitivity for concrete damage evaluation. Among these methods, nonlinear resonance acoustic methods have been widely used in laboratory tests on small scale test samples. In this study, a nonlinear impact-echo (IE) method for concrete damage evaluation was introduced, in which the IE frequency shift was measured at different impact force levels. The nonlinear IE test and the nonlinear impact resonance acoustic spectroscopy (NIRAS) test share similarities in the experimental setup and analysis method. However, the nonlinear IE test excites a local thickness resonance mode of a member instead of the global resonance vibration. Therefore, the analyzed mode is unaffected by member boundary conditions and is applicable to large concrete structural members. To identify the fundamental IE frequencies, multiple impacts were applied along the members. Once the fundamental IE frequencies were determined, the effectiveness of the nonlinear IE method was evaluated by testing seven concrete beam specimens with varying levels of alkali-silica reaction (ASR) damage. In conclusion, the nonlinear IE test demonstrates high sensitivity to concrete damage and allows for quantitative assessment of the damage state of concrete without a baseline measurement.

36 MATERIALS SCIENCE↗

System and method for acoustic to electronic transduction

According to various embodiments, a system for acoustic to electronic transduction is disclosed. The system includes a transducing medium configured to convert a received acoustic signal into a transduced electronic signal when a voltage is applied. The system further includes a high-pass filter coupled to the transducing medium, the high-pass filter configured to isolate the transduced signal from the applied voltage.

Carlsson, Johan A.↗

Online and noninvasive monitoring of battery health at negative-half cell in all-vanadium redox flow batteries using ultrasound

Hydrogen evolution is one of the major side reactions that is detrimental to the health of all-vanadium redox flow batteries, especially for long-term cycling. Effective, low-cost, and accurate online prediction and detection methods for hydrogen generation are not yet available. In this work, we designed an online, noninvasive ultrasonic probing approach for monitoring the state of charge (SoC), predicting the hydrogen generation, and detecting hydrogen gas bubbles in anolyte solutions. The technique employs a pulse-echo method to measure the sound speed and the acoustic attenuation coefficient of the anolyte solution. Through static offline experiments and online in operando experiments, we have demonstrated that when hydrogen gas is generated in anolyte solutions, large variations are observed in both sound speed and acoustic attenuation coefficient measurements. We found that the variations of acoustic attenuation coefficient are highly correlated (correlation coefficients >0.9) with the gas flow rate. In conclusion, the designed acoustic method can monitor the SoC of anolyte, predict the hydrogen generation, and detect the presence of gas bubbles in an anolyte solution and, thus, provide information about the state of health for operation and management of flow battery systems.

25 ENERGY STORAGE↗

Acoustic focusing of microplastics in microfabricated and steel tube devices: An experimental study on the effects from particle size and medium density

The contamination of aquatic systems with microplastics is becoming a severe threat to living organisms. Microplastics found in the environment consist of a wide range of particle sizes thus give rise to several challenges to current purification methods. Acoustic focusing has been utilized as a particle concentrating method in biological and non-biological applications. In this study, we investigate the feasibility of using acoustic focusing technology to isolate microplastics in aqueous samples and find that a two-stage mechanism is essential to concentrate microplastics of wide size ranges of the same material. We demonstrate for the first time experimentally that when the particle size is comparable to the resonance wavelength, they deviate from typical pressure nodal focusing and show pressure antinodal focusing. Further, we have expanded microplastics isolation beyond microfluidic systems and demonstrate their extraction capabilities relatively at higher flow rates using microplastics of polystyrene, polyethylene, and polymethyl methacrylate ranging from 6 µm to 300 µm diameter and in devices fabricated with cylindrical steel tubes. We could extract 70% of microplastics smaller than 180 µm and 82% of microplastics greater than 180 µm in diameter from an aqueous sample at a flow rate of 12 mL/min. In conclusion, we demonstrate that small microplastics focus on acoustic pressure nodes regardless of the medium density. However, focusing of large microplastics of the same material depends on the medium density.

42 ENGINEERING↗

Accobeam - Acoustic Collimated Beam

Researchers at Los Alamos have developed a collimated acoustic imaging technology for non-destructive characterization of highly attenuating media, such as concrete, rocks, mud, and metal casings. The technology is used to investigate structural defects that could compromise important assets such as well boreholes, building structures and sensitive materials like explosives. Current acoustic methods are often unable to penetrate such materials deeply for more informative analysis, which limits what can be learned about asset structural integrity, crucial information companies require to make investment decisions such as fixing or replacing structures well before assets are completely compromised. ACCObeam provides requisite penetration and high-resolution acoustic imaging with applications in borehole imaging, explosives threat evaluation, and structural health evaluation. This technology will potentially save companies significant money by producing accurate and useful visual analyses to better understand the actual condition of assets in the field and respond accordingly. Los Alamos is searching for a collaboration partner to develop this technology for commercial applications that will lead to licensing opportunities for broad industry deployment.

36 MATERIALS SCIENCE↗

An Acoustic Quench Detection Method for CICC Conductor Operating in Gas or Liquid

A method using micro-electro-mechanical system (MEMS) acoustic sensor technology to detect a quench and abnormal behavior of a superconductor is discussed. MEMS of microphone chips are installed along the coolant space of a superconducting conductor to monitor the coolant condition. By arranging the acoustic sensor array in a coolant flow channel, it is possible to quickly detect a change in the local condition of the coolant with precise spatial resolution. This method is useful for monitoring superconducting magnets and power cables, especially those made with a cable-in-conduit-conductor (CICC) configuration. The methodology described in this work will also be very useful for a high temperature superconducting (HTS) conductor, such as REBCO tape conductors.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Detection, monitoring, and determination of location of changes in metallic structures using multimode acoustic signals

Methods for detection, monitoring, and determination of location of changes in rigid structures with arbitrarily complex geometries are described. Implementations include locating acoustic transducers that generate and receive acoustic signals at multiple locations along a surface of the rigid structure, wherein longitudinal spacing between the transducer locations define measurement zones. Acoustic signals with chosen amplitude-time-frequency characteristics excite multiple vibration modes in the structure within each zone. Small mechanical changes in the inspection zones lead to scattering and attenuation of broadband acoustic signals, which are detectable as changes in received signal characteristics as part of a through-transmission technique. Additional use of short, narrowband pulse acoustic signals as part of a pulse-echo technique allows determination of the relative location of the mechanical change within each zone based on the differential delay profiles.

42 ENGINEERING↗

Computationally optimized and manufactured acoustic metamaterials

A method, system, and apparatus for fabricating an acoustic metamaterial is provided. In an embodiment, a method for fabricating an acoustic metamaterial includes determining at least one tuned physical property for each of a plurality of micro-resonators according to a desired acoustic property of the acoustic metamaterial. For a particular physical property, a value of the tuned physical property for at least one of the plurality of micro-resonators is different from a value of the tuned physical property for at least one other of the plurality of micro-resonators. The method also includes additively forming the acoustic metamaterial such that the acoustic metamaterial comprises a first structure and the plurality of micro-resonators embedded within the first structure. Forming the acoustic metamaterial is performed such that an actual physical property of each of the plurality of micro-resonators is equal to a corresponding tuned physical property for each of the plurality of micro-resonators.

Walsh, Timothy F.↗

Acoustic diagnostic system and method for superconducting devices operating in gas or liquid

A novel system and method for detecting a quench of a superconducting conductor and detecting abnormal behavior of a superconducting conductor using acoustic sensor technology in the coolant of a superconducting cable and/or magnet is disclosed. This system and method is not only limited to use for superconductors, but also may be used for any device disposed in liquid and gas. Acoustic sensors are installed along a coolant space of a superconducting conductor and monitor coolant conditions. By monitoring acoustic changes, temperature changes or coolant flow disruption can be detected very quickly by an acoustic sensor array. By disposition of the acoustic sensor array in a coolant flow channel, the acoustic sensor system can quickly detect a local condition, such as the thermal status (temperature) of a superconducting cable and magnet with precise spatial resolution.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Prediction of gas hydrate saturation using machine learning and optimal set of well-logs

We report resistivity and acoustic logs are widely used to estimate gas hydrate saturation in various sedimentary systems using one of the two popular methods ((1) acoustic velocity and (2) electrical resistivity), but the limitations of these two methods are often overlooked, which include (i) well-specific calibration of empirical exponents in the electrical resistivity method, (ii) assumption of known pore morphology for gas hydrates in the acoustic velocity method, and (iii) presence of unknown mineralogy and bulk modulus terms in the acoustic velocity method. NMR-density porosity-derived gas hydrate saturation based on the analysis of the transverse magnetization relaxation time (T2) is considered the most precise method, but acquisition of NMR-based logs is limited at relatively recent drilled sites; additionally, its use in conventional oil and gas reservoirs is not that common due to higher cost and operational deployment limitations associated with acquiring NMR well-logs. This study proposes a new method that predicts gas hydrate saturation (S h ) for any well using porosity, bulk density, and compressional wave (P wave) velocity well-logs with neural network (or stochastic gradient descent regression) without any well-specific calibration and/or other aforementioned shortcomings of the existing methods. The method is developed by examining the underlying dependency between S h and different combinations of well-logs, chosen from 6 routine logs, with 12 different machine learning (ML) algorithms. The accuracy of the proposed method in predicting S h is ~ 84%, which is better than the accuracy of seismic and electrical resistivity methods (≤ 75%) per the results reported by three different studies. The robustness of the method in the specific case of permafrost-associated gas hydrates is demonstrated with well-log data from two wells drilled on the Alaska North Slope.

58 GEOSCIENCES↗

Quantification of air leakage paths: a comparison of airflow and acoustic measurements

Today, the fan pressurization method is the most frequently used method to evaluate a building's airtightness. However, the localization and quantification of leaks remain difficult. In this paper, an acoustic method is introduced to estimate the leakage size of single leaks. Acoustic and airflow measurements were conducted and compared in laboratory tests within the same boundary conditions. This work aims to investigate if various leak sizes can be predicted using acoustic measurement methods. The test apparatus consists of two chambers, separated by a test wall. This wall represents a single characteristic air leakage path. Various types of wall structures with different slit geometries, wall thicknesses, and insulation materials were investigated. The acoustic measurements were performed with a sound source placed in one chamber and ultrasonic microphones located in both chambers. These results were compared to measured airflows through the test wall to provide estimates of uncertainty in the acoustic approach, which indicate a linear trend. Finally, these laboratory measurements were compared to the same measurements at a real office building. Finally, although the acoustic measurement uncertainty is still significant (greater than +/- 50%), the acoustic method has the potential to give an order of magnitude of single leak sizes.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Acoustic signal based analysis of batteries

Systems and methods for acoustic signal based analysis, include obtaining acoustic response signal data of at least a portion of a battery cell, the acoustic response signal data comprising waveforms generated by transmitting one or more acoustic excitation signals into at least the portion of the battery cell and recording response vibration signals to the one or more acoustic excitation signals. One or more metrics are determined from at least the acoustic response signal data, the one or more metrics being determined based on correlation of the one or more metrics to one or more characteristics of battery cells and a reference model is generated from the one or more metrics. A test battery can be evaluated using the reference model. Actionable insights or recommendations can be generated based on the evaluation. The reference model can also be updated based on the evaluation.

Van Tassell, Barry J.↗

Mapping of Benthic Habitats at Marine Renewable Energy Sites Using Multibeam Echosounder and Sediment Profile Imaging Technologies

Objectives/Scope: The goal of this work was to develop a consistent and semi-automated seafloor survey method for generating high-resolution, benthic habitat maps for environmental assessments and monitoring of marine renewable energy sites. Sediment profile and plan view imaging (SPI/PV) technology was combined with multibeam bathymetry and acoustic backscatter methods to demonstrate a rapid, cost-effective benthic mapping protocol. A key technical innovation of this project was the development of an image processing platform that automatically measures key features from the images. Methods: Multibeam echosounder (MBES) acoustic and SPI/PV surveys were conducted at three coastal areas off the U.S. west coast, including the PacWave South energy test site off of Newport, Oregon. Point data on physical and biological sediment conditions obtained from the SPI/PV imagery were used to efficiently ground-truth the high-resolution MBES bathymetry and backscatter mosaics. The SPI camera is an optical corer that obtains an undisturbed 21 by 15 cm, high-resolution, cross-sectional image of the sediment–water interface and upper sediment column. The plan view camera attached to the SPI camera frame captures a downward looking view of the seabed immediately before the SPI image is obtained. As part of this project, we developed a computer vision image processing platform (iSPI) that uses deep convolutional neural networks and other approaches to automatically identify and measure key features in the images, such as grain size and surface relief. Results: Detailed benthic habitat maps were generated using this mapping approach for three different marine settings. The sites mapped included a silt-dominated embayment; a sloping, nearshore, transitional very fine to coarse sand bottom; and a medium, sand-dominated continental shelf marine energy test site. In each case, acoustic and imaging surveys were completed in less than week, and detailed benthic habitat maps were generated within 60 days. The results were placed within the Coastal and Marine Ecological Classification Standard (CMECS) habitat mapping framework, and various combinations of the bathymetry, backscatter, and SPI and PV data were used to generate CMECS component maps. Novel Information: This project developed and demonstrated a repeatable and cost-effective approach for efficiently mapping benthic habitat conditions over broad areas of the seafloor by combining state-of-the-art acoustic and imaging techniques. The primary survey tools used in this project have been used previously in both the offshore renewable (wind) and oil and gas sectors to map and monitor benthic environments. This project’s innovations include 1) the focused use of high-resolution SPI/PV imagery to ground-truth acoustic mosaics, and 2) the development of a computer automated image analysis processing tool that both streamlines and standardizes the generation of data from the imagery and makes the data extraction process more cost-effective and repeatable.

13 HYDRO ENERGY↗

Determination of acoustic nonlinearity parameters using thermal modulation of ultrasonic waves

This study presents a test method and its theoretical framework to determine the acoustic nonlinearity parameters (α, β, δ) of material using thermal modulation of ultrasonic waves. Temperature change-induced thermal strain excites the nonlinear response of the material and modulates the ultrasonic wave propagating in it. Experimental results showed a strong correlation between the relative wave velocity change and the temperature change. With a quadratic polynomial model, the acoustic nonlinearity parameters were obtained from the polynomial coefficients by curve fitting the experimental data. Their effects on thermal-induced velocity change were discussed. The parameters α, β, and δ govern the hysteretic gap, average slope, and curvature of the correlation curve, respectively. The proposed theory was validated on aluminum, steel, intact and damaged concrete samples. The obtained nonlinear parameters show reasonable agreement with values reported in the literature. Compared to other nonlinear acoustic methods using vibration or acoustic excitation, the thermal modulation method generates more uniform, slow changing, and larger strain field in the test sample. Employing the thermal effect as the driving force for nonlinearity instead of an undesired influencing factor, this method can measure the absolute values of α, β, and δ with good accuracy using a simple ultrasonic test setup.

Physics↗