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At least 19 records

A Ratio of Spore to Viable Organisms: A Case Study of the JPL-SAF Cleanroom

Spacecraft surfaces that are destined to land on potential life-harboring celestial bodies are required to be rigorously cleaned and continuously monitored for spore bioburden as a proxy for spacecraft cleanliness. The NASA standard assay (NSA), used for spacecraft bioburden estimates, specifically measures spores that are cultivable, aerobic, resistant to heat shock, and grow at 30 C in a nutrient-rich medium. Since the vast majority of microorganisms cannot be cultivated using the NSA, it is necessary to utilize state-of-the art molecular techniques to better understand the presence of all viable microorganisms, not just those measured with the NSA. In this study, the nutrient-deprived low biomass cleanrooms, where spacecraft are assembled, were used as a surrogate for spacecraft surfaces to measure the ratio of NSA spores in relation to the total viable microorganism population in order to make comparisons with the 2006 Space Studies Board (SSB) estimate of 1 spore per approximately 50,000 viable organisms. Ninety-eight surface wipe samples were collected from the Spacecraft Assembly Facility (SAF) cleanroom at the Jet Propulsion Laboratory (JPL) over a 6-month period. The samples were processed and analyzed using classical microbiology along with molecular methodology. Traditional microbiology plating methods were used to determine the cultivable bacterial, fungal, and spore populations. Molecular assays were used to determine the total organisms (TO, dead and live) and the viable organisms (VO, live). The TO was measured using adenosine triphosphate (ATP) and quantitative polymerase chain reaction (qPCR) assays. The VO was measured using internal ATP, propidium monoazide (PMA)-qPCR, and flow cytometry (after staining for viable microorganisms) assays. Based on the results, it was possible to establish a ratio between spore counts and VO for each viability assay. The ATP-based spore to VO ratio ranged from 149-746, and the bacterial PMA-qPCR assay-based ratio ranged from 314-1,491 VO, per spore. The most conservative estimate came from fluorescent-assisted cell sorting (FACS), which estimated the ratio to be 12,091 VO per 1 NSA spore. Since archaeal (less than 1%) and fungal (approximately 2%) populations were negligible, the spore to VO ratios were based on bacterial population estimates. The most conservative ratio from this study can be used as a replacement for the SSB estimate on nutrient-deprived (oligotrophic) desiccated spacecraft surfaces, to estimate the VO from NSA measurements without utilizing state-of-the art molecular methods that are costly and require more biomass than is typically found on spacecraft surfaces.

Hendrickson, Ryan

Modification of Mach 6 Freestream Flow by Pitot Probe Bow Shock as Measured by Line FLDI and SAFS

Measurements of the flow in front of flat-face Pitot probes in NASA Langley ResearchCenter’s 20-Inch Mach 6 Air Tunnel were made using a 35-point line focused laser differentialinterferometer (FLDI), a two-point FLDI, and a self-aligned focusing schlieren system. Thesesystems were used to make measurements of flow unsteadiness between the bow shock andPitot probe face, while also probing the freestream region just upstream of the bow shock.Shock standoff distances from the non-intrusive measurements match closely with analyticalsolutions using the probe face diameter. Acoustic wave angles and velocities in the freestreamcompare favorably with DNS simulations of a Mach 6 freestream flow, and wave velocities in thepost-shock region slow as they approach the probe face. Peak frequencies in the post-shockregion as measured by FLDI compare favorably to the estimates computed from the shockstandoff distance for most probe diameters. With increasing unit Reynolds number, fluctuationintensities in the post-shock region were attenuated below the intensities of the freestream, witha spectral, spatial, and probe size dependence. Pitot probe power spectral density estimatesare presented from the test campaign, which can be compared with FLDI and SAFS data toevaluate the applicability of the transfer functions derived from computational simulations.

Joshua M Weisberger

Additional Aviation Sustainability Strategies

Emissions from the aviation sector are expected to grow significantly in the next few decades due to growing demand for aviation. The U.S. fleet size has increased significantly in the past 20 years, growing from 2,132 aircraft to 5,485 aircraft. Similarly from 1990 to 2019, aviation emissions more than doubled, growing faster than any other transportation mode. These emissions include carbon dioxide (CO2), as well as non-CO2 sources including nitrogen oxides (NOx), sulfur dioxide (SO2), water (H2O), and particulate matter (soot). However, this report focuses specifically on CO2 emissions because the impacts from CO2 are well-understood and long-lived. There are also many climate goals tied to CO2 emissions, including the US Aviation Climate Action Plan which is pushing for net-zero in 2050. CO2 is a greenhouse, or heat-trapping, gas that comes from the extraction and burning of fossil fuels, from wildfires, and natural processes like volcanoes. Greenhouse gases absorb heat radiating from the Earth’s surface and release it in all directions, including back toward Earth’s surface. By adding more CO2 to the atmosphere, humans amplify the natural greenhouse effect, causing global temperature to rise. Since the onset of industrial times in 1750, human activities have raised atmospheric CO2 by 50%. Considering this challenge, the United States has set a climate goal to achieve net-zero CO2 emissions in the U.S. aviation sector by 2050 to reduce the environmental impact of aviation. Net-zero emissions refers to the amount of greenhouse gases (GHGs), such as CO2, methane or SO2, that are removed from the atmosphere being equal to those emitted by human activity. This contrasts with carbon-neutrality, which refers to no net release of carbon dioxide to the atmosphere, especially through offsetting emissions, that could be accomplished through a series of actions. As of 2022, the goal of net-zero has been adopted by much of the aviation industry including multiple airlines and countries. To reach net-zero by 2050, sustainable aviation fuels (SAFs) are identified as the technology that could provide the greatest impact within this timeframe. However, certain challenges may limit the production and/or adoption of SAFs in the marketplace, such as competition for feedstocks, production capacity, and high cost. In this case, SAFs would be insufficient to meet the net-zero CO2 emission goals. Therefore, in addition to SAFs, other strategies will be needed to close the gap on emissions produced as a result of SAF shortfalls or to enhance the impact of SAFs on reducing emissions. NASA’s Aeronautics Research Mission Directorate (ARMD) aims to examine these additional pathways to explore how its research may best contribute to the realization of net-zero emissions by 2050. Specifically, this effort considers strategies that can reduce emissions in the aviation sector in combination with the use of SAFs and carbon offsets while still meeting projected passenger demand. The strategies considered are not only feasible but also could be operationally scalable by 2050. The goal of this report is to investigate additional strategies such as alternative flight and ground operations, providing insight into the CO2 emission reduction potential and feasibility of strategy implementation by 2050.

Carbon Emissions

Recent Progress and Development of Self-Aligned Focusing Schlieren

A broad range of developments and applications of self-aligned focusing schlieren (SAFS)systems is presented. The replacement of the Ronchi ruling (RR) with a𝜇LCD was demonstrated with digital SAFS, which provides the ability to modulate the pattern and orientation without physical intervention. Additionally, replacement of the traditional RR with spectral-spatial filters was demonstrated to yield the ability to simultaneously image horizontal and vertical index of refraction gradients using two separate wavelengths. The application of the Scheimpflug principle to SAFS systems enables off-axis imaging and allowed for extended fields-of-view to be captured using a two-camera system. The addition of a plenoptic camera to a baseline SAFS system enables refocusing capabilities, which is demonstrated on two jets offset along the optical axis. Furthermore, application of an event-based camera to SAFS was shown to enable acquisition of sparse, spatio-temporal data. Beyond the listed developments, SAFS was applied to difficult and constraining facilities, such as the National Full-Scale Aerodynamics Complex(NFAC) 80- by 120-ft Wind Tunnel and the Thermal Acoustic Engines (TAE) rig at NASA Ames Research Center and NASA Glenn Research Center, respectively. Behavior of Ronchi rulings, Rochon prisms, and the use of quarter-wave plates versus quarter-wave films are evaluated to provide insight for future experimental design of SAFS-systems.

Self-Aligned Focusing Schlieren

Recent Progress and Development of Self-Aligned Focusing Schlieren

A broad range of developments and applications of self-aligned focusing schlieren (SAFS)systems is presented. The replacement of the Ronchi ruling (RR) with a𝜇LCD was demonstrated with digital SAFS, which provides the ability to modulate the pattern and orientation without physical intervention. Additionally, replacement of the traditional RR with spectral-spatial filters was demonstrated to yield the ability to simultaneously image horizontal and vertical index of refraction gradients using two separate wavelengths. The application of the Scheimpflug principle to SAFS systems enables off-axis imaging and allowed for extended fields-of-view to be captured using a two-camera system. The addition of a plenoptic camera to a baseline SAFS system enables refocusing capabilities, which is demonstrated on two jets offset along the optical axis. Furthermore, application of an event-based camera to SAFS was shown to enable acquisition of sparse, spatio-temporal data. Beyond the listed developments, SAFS was applied to difficult and constraining facilities, such as the National Full-Scale Aerodynamics Complex(NFAC) 80- by 120-ft Wind Tunnel and the Thermal Acoustic Engines (TAE) rig at NASA Ames Research Center and NASA Glenn Research Center, respectively. Behavior of Ronchi rulings, Rochon prisms, and the use of quarter-wave plates versus quarter-wave films are evaluated to provide insight for future experimental design of SAFS-systems.

Self-Aligned Focusing Schlieren

Self-Aligned Focusing Schlieren at the 0.3-M Transonic Cryogenic Tunnel and the National Transonic Facility

The implementation of a self-aligned focusing schlieren (SAFS) system at two cryogenic wind tunnels at NASA Langley Research Center is discussed. Risk-reduction testing of the SAFS system was first performed at the 0.3-M Transonic Cryogenic Tunnel to evaluate the system’s operation in a small-scale characteristic cryogenic facility. Testing was conducted with three models: a three-quarter span 25.4-mm-diameter cylinder, a semi-span 65A006 tapered unswept airfoil, and a full-span SC(3)-0712 airfoil. Testing with the cylinder and semi-span airfoil revealed a highly dynamic shock environment, whereas the shock on the full-span airfoil was stationary, solidifying the usage of this model for a pre-/post-shock particle tracking velocimetry measurement. Temperature-induced polarization-altering window stresses were encountered during low-temperature testing, and were mitigated using a “non-ideal” quartz/quartz Rochon prism that had largely been neglected since the SAFS system’s first introduction in favor of the more favorable “ideal” glass/quartz Rochon prism. The size of the SAFS system was then decreased in order to fit inside an environmentally-controlled camera can enclosure at the National Transonic Facility (NTF) for testing of a sting-mounted aircraft model. The SAFS system was demonstrated to be effective at filtering out the large density gradient flow in the 0.3-M plenum, and the thick, high density turbulent boundary layers on the wind tunnel walls at the NTF. Results of the testing campaigns and improvements to future systems are discussed.

Joshua M. Weisberger

Self-Aligned Focusing Schlieren at the 0.3-M Transonic Cryogenic Tunnel and the National Transonic Facility

The implementation of a self-aligned focusing schlieren (SAFS) system at two cryogenic wind tunnels at NASA Langley Research Center is discussed. Risk-reduction testing of the SAFS system was first performed at the 0.3-M Transonic Cryogenic Tunnel to evaluate the system’s operation in a small-scale characteristic cryogenic facility. Testing was conducted with three models: a three-quarter span 25.4-mm-diameter cylinder, a semi-span 65A006 tapered unswept airfoil, and a full-span SC(3)-0712 airfoil. Testing with the cylinder and semi-span airfoil revealed a highly dynamic shock environment, whereas the shock on the full-span airfoil was stationary, solidifying the usage of this model for a pre-/post-shock particle tracking velocimetry measurement. Temperature-induced polarization-altering window stresses were encountered during low-temperature testing and were mitigated using a “non-ideal” quartz/quartz Rochon prism that had largely been neglected since the SAFS system’s first introduction in favor of the more favorable “ideal” glass/quartz Rochon prism. The size of the SAFS system was then decreased in order to fit inside an environmentally controlled camera can enclosure at the National Transonic Facility (NTF) for testing of a sting-mounted aircraft model. The SAFS system was demonstrated to be effective at filtering out the large density gradient flow in the0.3-M plenum, and the thick, high density turbulent boundary layers on the wind tunnel walls at the NTF. Results of the testing campaigns and improvements to future systems are discussed.

Joshua M Weisberger

Compositional Grading in an Impact-produced Spherule Bed, Barberton Greenstone Belt, South Africa: A Key to Condensation History of Rock Vapor Clouds

The chemical and physical processes by which spherules form during the condensation of impact-produced rock vapor clouds are poorly understood. Although efforts have been made to model the processes of spherule formation, there is presently a paucity of field data to constrain the resulting theoretical models. The present study examines the vertical compositional variability in a single early Archean spherule bed in the Barberton Greenstone Belt (BGB), South Africa, in order to better identify the process by which impact vapor clouds condense and spherules form and accumulate. The BGB spherule beds are suitable for this type of study because of their great thickness, often exceeding 25cm of pure spherules, due to the massive sizes of the impactors. Two main problems complicate analysis of vertical compositional variability of graded spherule beds: (1) differential settling of particles in both the vapor and water column due to density and size differences and (2) turbulence within the vapor cloud. The present study compares sections of spherule bed S3 from four different depositional environments in the Barberton Greenstone Belt: (1) The Sheba Mine section (SAF-381) was deposited under fairly low energy conditions in deep water, providing a nice fallout sequence, and also has abundant Ni-rich spinels; (2) Jay's Chert section (SAF-380) was deposited in subaerial to shallow-water conditions with extensive post-depositional reworking by currents. The spherules also have preserved spinels; (3) the Loop Road section (loc. SAF-295; samp. KSA-7) was moderately reworked and has only rare preservation of spinels; and (4) the shallow-water Barite Syncline section (loc. SAF-206; samp KSA-1) has few to no spinels preserved and is not reworked. Although all of the spherule beds have been altered by silica diagenesis and K-metasomatism, most of the compositional differences between these sections appear to reflect their diagenetic histories, possibly related to their differing depositional environments. Sulfate diagenesis in the Barite Syncline and Loop road sections may account for the loss of spinels.

Krull, A. E.

Establishing a Pathway to Greener Aviation

The burning and production of jet fuel in both commercial and regional planes at a high volume releases extreme levels of carbon emission as of its current state. This paper analyzes the implementation and benefits of three sustainable alternatives to traditional jet fuel: sustainable aviation fuel (SAF), hydrogen fuel cells, and electric batteries. A roadmap to show progress in reducing the carbon footprint of aviation is then proposed. The first phase of the roadmap involves the evolution of technology, validation, and testing of SAF-powered planes and the preliminary development of hydrogen production and propulsion and battery technology and the development of electric propulsion technologies. The second phase illustrates the widespread implementation of all three fuel sources where electric aviation will be enforced in short-range travel (30 min-3 hrs), medium-range aircraft (3-6 hrs) will use hydrogen fuel cells, and long-range (6+ hrs) airliners will utilize SAF. The third and final phase will enact the use of SAF and electric batteries for long-range applications while hydrogen fuel cells will dominate short- and medium-range aircraft. This roadmap, in combination with proper legislative action and corporate cooperation paired with public awareness, can help eliminate the usage of jet fuel in the aviation industry and replace it with three more sustainable and clean fuel sources.

green aviation

The Standard Autonomous File Server, a Customized, Off-the-Shelf Success Story

The Standard Autonomous File Server (SAFS), which includes both off-the-shelf hardware and software, uses an improved automated file transfer process to provide a quicker, more reliable, prioritized file distribution for customers of near real-time data without interfering with the assets involved in the acquisition and processing of the data. It operates as a stand-alone solution, monitoring itself, and providing an automated fail-over process to enhance reliability. This paper will describe the unique problems and lessons learned both during the COTS selection and integration into SAFS, and the system's first year of operation in support of NASA's satellite ground network. COTS was the key factor in allowing the two-person development team to deploy systems in less than a year, meeting the required launch schedule. The SAFS system his been so successful, it is becoming a NASA standard resource, leading to its nomination for NASA's Software or the Year Award in 1999.

Semancik, Susan K.

Molecular microbial diversity of a spacecraft assembly facility

In ongoing investigations to map and archive the microbial footprints in various components of the spacecraft and its accessories, we have examined the microbial populations of the Jet Propulsion Laboratory's Spacecraft Assembly Facility (JPL-SAF). Witness plates made up of spacecraft materials, some painted with spacecraft qualified paints, were exposed for approximately 7 to 9 months at JPL-SAF and examined the particulate materials collected for the incidence of total cultivable aerobic heterotrophs and heat-tolerant (80 degrees C for 15-min.) spore-formers. The results showed that the witness plates coated with spacecraft qualified paints attracted more dust particles than the non-coated stainless steel witness plates. Among the four paints tested, witness plates coated with NS43G accumulated the highest number of particles, and hence attracted more cultivable microbes. The conventional microbiological examination revealed that the JPL-SAF harbors mainly Gram-positive microbes and mostly spore-forming Bacillus species. Most of the isolated microbes were heat resistant to 80 degrees C and proliferate at 60 degrees C. The phylogenetic relationships among 23 cultivable heat-tolerant microbes were examined using a battery of morphological, physiological, molecular and chemotaxonomic characterizations. By 16S rDNA sequence analysis, the isolates fell into seven clades: Bacillus licheniformis, B. pumilus, B. cereus, B. circulans, Staphylococcus capitis, Planococcus sp. and Micrococcus lylae. In contrast to the cultivable approach, direct DNA isolation, cloning and 16S rDNA sequencing analysis revealed equal representation of both Gram-positive and Gram-negative microorganisms.

Industry

Utilizing Radiocarbon and Thermo-optical Measurements to Quantify Source Contributions for Aircraft Engine Particulate Emissions

Recent technological advancements in aircraft engine technology and fuel sources have promising potential for reducing particulate emissions from aircraft engines. During the 2022 Boeing ecoDemonstrator ground-based field campaign, particle and trace gas emissions were sampled for four different fuels, including 100% sustainable aviation fuel (SAF), petroleum-based Jet A fuel, petroleum-based Jet A low sulfur fuel, and a SAF blend. Particulate emissions were collected on quartz microfiber filters and analysed for their radiocarbon content and their elemental carbon (EC) and organic carbon (OC) concentrations. In addition, the radiocarbon content of Jet A fuel, SAF, and aircraft engine oil was also measured. We used the radiocarbon content of the particulate emissions samples and source end members to quantify the relative contribution from engine oil and fuel to carbonaceous aerosol emissions. The results from this study can be used to understand how the implementation of technological advancements in aircraft engine technology and fuel sources will likely impact future aircraft particulate emissions.

Liz Wiggins

Implementation of a Compact Self-Aligned Focusing Schlieren System for Measurements with the SBLT-II Flight Test Article

Previous flight testing of a supersonic natural laminar flow airfoil on the NASA F-15Baircraft showed evidence of multiple impinging shocks (shock train) of unknown origin, which generated sufficient crossflow on the airfoil to affect the primary measurement goal of transition location. A compact self-aligned focusing schlieren (SAFS) system is being developed to visualize this shock train on the forward surface of the Supersonic Boundary Layer Transition - PhaseII (SBLT-II) flight test article. Details of the design of the SAFS flight system, the constraints imposed by the imaging pod that will be mounted to the F-15B aircraft, preliminary laboratory results obtained with the system, and challenges associated with integrating the imaging system into the aircraft are presented. The resulting schlieren images obtained from this flight test will allow for a three-dimensional reconstruction of the unknown shock structure, validating the use of SAFS for flight testing.

Joshua M. Weisberger

Self-Aligned Focusing Schlieren and OH Planar Laser-Induced Fluorescence Flow Visualization in a Dual-Mode Scramjet

Ahigh-speed self-aligned focusing schlieren (SAFS) system was used to visualize density gradients in and around the cavity flameholder of the combustor section of the University of Virginia Supersonic Combustion Facility (UVASCF). Images with this system were acquired at a framing rate of 110 kHz with no fuel injection, with fuel injection but no flame, and for fuel injection with combustion corresponding to a global equivalence ratio of 𝜙 = 0.18. Images with an air throttle in operation to modify the shock train location with fuel injection and with flame were also acquired. Simultaneous OH planar laser-induced fluorescence (PLIF) images were also acquired at a framing rate of 20 Hz. Results obtained with both visualization techniques are compared to one another to highlight how SAFS can complement more advanced flow visualization techniques and resolve dynamic behavior that may not otherwise be captured. Both proper orthogonal decomposition (POD) and dynamic mode decomposition (DMD) analysis techniques are applied to the SAFS image sequences to identify coherent periodic structures for the runs with fuel injection and combustion.

Brett F Bathel