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At least 19 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Controlled electrochemical nutrient delivery to enhance marine primary productivity

Oceanic photosynthesis contributes to approximately half of the Earth’s net annual primary productivity. Marine photosynthetic productivity has a high degree of heterogeneity due to spatial and temporal co-limitations of light, temperature, and/or nutrients. Across coastal, near-shore, and open ocean regions, insufficient concentrations of key nutrients (e.g., N, P, Fe) can limit primary productivity. Although studies have shown a significant increase in primary productivity with the addition of low doses of trace nutrients, a sustainable approach to reliably deliver and maintain low doses of nutrients and ensure their bioavailability, remains a challenge. Chemical nutrient addition has relied on the use of chelating agents to ensure nutrient bioavailability, but synthetic chelators are persistent environmental pollutants. In this study, we demonstrate for the first time the use of a controlled electrochemical nutrient delivery (CEND) approach to accelerate the growth of phytoplankton without the need for such chelators. Our study uses commercial stainless-steel electrodes to deliver low concentrations of iron to enhance growth rates in the microalga Picochlorum celeri TG2. To demonstrate the process control offered by the CEND method, we evaluate iron delivery as a function of pulse time, pulse frequency, and rest time between pulses. Our data show that at the same total Fe dose of 163 ppb, electrochemical iron delivery can achieve 9.54 ± 0.58 mg biomass/µg Fe, which is comparable to 9.14 ± 0.17 mg biomass/µg Fe achieved with chemical iron additions that include the synthetic chelating agent ethylenediaminetetraacetic acid (EDTA). Further, when different total iron doses (163 ppb, 325 ppb, and 650 ppb) were delivered over 72 h using CEND, biomass yield per iron dose was higher at lower doses: 9.54 ± 0.58 mg biomass/µg Fe at 163 ppb vs. 4.32 ± 0.32 mg biomass/µg Fe at 650 ppb. This highlights the benefits of CEND in delivering frequent and low doses of nutrients for improved process efficiency. Preliminary assessments show both lower cost and reduced greenhouse gas emissions from electrochemical over chemical iron additions with EDTA. The CEND approach opens new pathways to enhance marine primary productivity, without the unintended environmental impacts of synthetic chelators.

analytical chemistry↗
DOE OSTI2025

Porewater chemistry in Typha-dominated brackish tidal marsh, PIE LTER, Plum Island Sound, MA, July 2022–September 2024

This dataset contains profile measurements of porewater constituents taken on 3-4 days across the growing seasons in 2022, 2023, and 2024 in a tidal brackish marsh within the Plum Island Ecosystems Long Term Ecological Research site (PIE LTER), located in the Plum Island Sound, Massachusetts (MA). Measurements were taken to monitor changes in porewater chemistry induced by seasonal saltwater intrusion at the site. Samples were taken in two locations: one was close to the creek bank and the other in the marsh interior. Water was sampled from 2-5 depths between the surface to 50cm using a sipper consisting of a hollow stainless steel rod with an opening at the end similar to that described in (Berg & McGlathery, 2001). The rod was pushed into the sediment to the desired depth, typically every 10cm, and water samples were taken by syringe. Water was not obtained at all depths. Samples were preserved and analyzed in the lab. Metadata files Typha_porewater_sipper_dd.csv and Typha_porewater_sipper_flmd.csv contain detailed information on data variables, sampling and QA/QC methods, and site location.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

A global comparison of surface and subsurface microbiomes reveals large-scale biodiversity gradients, and a marine-terrestrial divide

Subsurface environments are among Earth’s largest habitats for microbial life. Yet, until recently, we lacked adequate data to accurately differentiate between globally distributed marine and terrestrial surface and subsurface microbiomes. Here, we analyzed 478 archaeal and 964 bacterial metabarcoding datasets and 147 metagenomes from diverse and widely distributed environments. Microbial diversity is similar in marine and terrestrial microbiomes at local to global scales. However, community composition greatly differs between sea and land, corroborating a phylogenetic divide that mirrors patterns in plant and animal diversity. In contrast, community composition overlaps between surface to subsurface environments supporting a diversity continuum rather than a discrete subsurface biosphere. Differences in microbial life thus seem greater between land and sea than between surface and subsurface. Diversity of terrestrial microbiomes decreases with depth, while marine subsurface diversity and phylogenetic distance to cultured isolates rivals or exceeds that of surface environments. We identify distinct microbial community compositions but similar microbial diversity for Earth’s subsurface and surface environments.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Plant Roots and the Fate of Mineral-Associated Soil Carbon (Final Report)

Atmospheric CO 2 concentrations continue to rise due to human activities, but there are also natural mechanisms operating in ecosystems that can store carbon in soil long-term. The organic carbon derived from organisms in soil becomes associated with minerals, where it can be protected for thousands of years. Plants are particularly important contributors of carbon belowground, via their roots. The long-term protection of carbon contributed to soil has great value to humans as climate change looms. But, to keep growing, living plants and soil microbes also need to be able to access and recycle soil nutrients that are stored along with the carbon. In this DOE-funded work, Cardon (MBL), Keiluweit (UMass Amherst/University of Lausanne), Malmstrom (MSU), and Riley (LBNL) used lab and greenhouse experiments coupled with mathematical modeling to determine mechanisms by which plant roots and their associated microbes can dislodge organic matter from soil minerals, making its components available for recycling, but also making its carbon vulnerable to re-release to the atmosphere as CO 2 .

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Sponge exhalent metabolites influence coral reef picoplankton dynamics

Coral reef sponges efficiently take up particulate and dissolved organic matter (DOM) from the water column and release compounds such as nucleosides, amino acids, and other dissolved metabolites to the surrounding reef via their exhalent seawater, but the influence of this process on reef picoplankton and nutrient processing is relatively unexplored. Here we examined the impact of sponge exhalent on the reef picoplankon community and subsequent alterations to the reef dissolved metabolite pool. We exposed reef picoplankton communities to a sponge exhalent water mixture (Niphates digitalis and Xestospongia muta) or filtered reef seawater (control) in closed, container-based dark incubations. We used 16S rRNA gene sequencing and flow cytometry-based cell counts to examine the picoplankton community and metabolomics and other analyses to examine the dissolved metabolite pool. The initial sponge exhalent was enriched in adenosine, inosine, chorismate, humic-like and amino acid-like components, and ammonium. Following 48 h of exposure to sponge exhalent, the picoplankton differed in composition, were reduced in diversity, showed doubled (or higher) growth efficiencies, and harbored increased copiotrophic and denitrifying taxa (Marinomonas, Pontibacterium, Aliiroseovarius) compared to control, reef-water based incubations. Alongside these picoplankton alterations, the sponge treatments, relative to seawater controls, had decreased adenosine, inosine, tryptophan, and ammonium, metabolites that may support the observed higher picoplankton growth efficiencies. Sponge treatments also had a net increase in several monosaccharides and other metabolites including anthranilate, riboflavin, nitrite, and nitrate. Our work demonstrates a link between sponge exhalent-associated metabolites and the picoplankton community, with exhalent water supporting an increased abundance of efficient, copiotrophic taxa that catabolize complex nutrients. The copiotrophic taxa were often different from those observed in previous algae and coral studies. These results have implications for better understanding the multifaceted role of sponges on picoplankton biomass with subsequent potential impacts to coral and other planktonic feeders in oligotrophic reef environments.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Vulnerability of mineral-organic associations in the rhizosphere

The majority of soil carbon (C) is stored in organic matter associated with reactive minerals. These mineral-organic associations (MOAs) inhibit microbial and enzymatic access to organic matter, suggesting that organic C within MOAs is resistant to decomposition. However, plant roots and rhizosphere microbes are known to transform minerals through dissolution and exchange reactions, implying that MOAs in the rhizosphere can be dynamic. Here we identify key drivers, mechanisms, and controls of MOA disruption in the rhizosphere and present a new conceptual framework for the vulnerability of soil C within MOAs. We introduce a vulnerability spectrum that highlights how MOAs characteristic of certain ecosystems are particularly susceptible to specific root-driven disruption mechanisms. This vulnerability spectrum provides a framework for critically assessing the importance of MOA disruption mechanisms at the ecosystem scale. Comprehensive representation of not only root-driven MOA formation, but also disruption, will improve model projections of soil C-climate feedbacks and guide the development of more effective soil C management strategies.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration

Specialized or secondary metabolites are small molecules of biological origin, often showing potent biological activities with applications in agriculture, engineering and medicine. Usually, the biosynthesis of these natural products is governed by sets of co-regulated and physically clustered genes known as biosynthetic gene clusters (BGCs). To share information about BGCs in a standardized and machine-readable way, the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard and repository was initiated in 2015. Since its conception, MIBiG has been regularly updated to expand data coverage and remain up to date with innovations in natural product research. Here, we describe MIBiG version 4.0, an extensive update to the data repository and the underlying data standard. In a massive community annotation effort, 267 contributors performed 8304 edits, creating 557 new entries and modifying 590 existing entries, resulting in a new total of 3059 curated entries in MIBiG. Particular attention was paid to ensuring high data quality, with automated data validation using a newly developed custom submission portal prototype, paired with a novel peer-reviewing model. MIBiG 4.0 also takes steps towards a rolling release model and a broader involvement of the scientific community. MIBiG 4.0 is accessible online at https://mibig.secondarymetabolites.org/.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Data from a four-day long microcosm experiment addressing the destabilization of artificial mineral-associated organic matter by model root exudates embedded in a soil matrix from the Rocky Mountain Biological Laboratory (Gothic, CO, USA), 2019

This dataset provides data collected during a four-day long laboratory soil microcosm experiment testing the efficacy of root exudate-driven mineral-associated organic matter destabilization. This dataset contains four data files in comma-separate values (*.csv). The files provide the metadata and the experimental results on microbial respiration, MAOM-derived respiration, and sequential mineral-extractions. This data was used to produce the figures in Bölscher et al., 2026. The results of the experiment can be found in the open access article Bölscher et al., 2026 (https://doi.org/10.1016/j.soilbio.2026.110276). Abstract: Mineral-associated organic matter (MAOM) is often considered stable, but root exudates can destabilize MAOM via various pathways. Theory and model system studies suggest that direct MAOM destabilization by strong ligands, like oxalic acid, or reducing agents, like catechol, is more effective than indirect, microbial-mediated MAOM destabilization, stimulated by less reactive compounds like glucose. Here, we demonstrate that the presence of a soil matrix alters the efficacy of exudate-driven MAOM destabilization pathways. Glucose and catechol destabilized significantly greater amounts of MAOM from ferrihydrite and aluminum hydroxide (Al (OH)3) embedded in a soil matrix than oxalic acid. Our findings indicate that indirect, microbial-mediated MAOM destabilization may play a larger role than direct MAOM destabilization in soil environments.

Destabilization↗
DOE OSTI2025

From tides to seasons: How cyclic tidal drivers and plant physiology interact to affect carbon cycling at the terrestrial-estuarine boundary (Final technical report)

Coastal ecosystems are among the most biologically and biogeochemically active and diverse systems on Earth. Because they act as important linkages between terrestrial ecosystems and the open ocean, their incorporation in Earth system models (ESMs) is critical to predict coastal and global responses to environmental changes. However, they vary greatly in the magnitude of tides and the volume and timing of freshwater input from land, making it challenging to model the major biogeochemical reactions that control productivity and greenhouse gas emissions across coastal terrestrial aquatic interfaces (TAIs). Our overall objective was to improve mechanistic process understanding and modeling of tidal wetland hydro-biogeochemistry in coastal TAIs. We established a new flux tower site (Ameriflux US-PLo) in the oligohaline part of the Parker River to continuously monitor ecosystem-scale carbon fluxes under temporally varying salinity conditions. The site is co-located with long-term monitoring plots of the Plum Island Ecosystems LTER project. We installed wells and redox sensors in the marsh interior and creek bank, established biomass monitoring plots and deployed novel optode sensors in both locations. We used this data to parameterize plant-mediated transport in PFLOTRAN and tested the impact of soil heterogeneity on porewater constituents and gas fluxes. We collected observations of root oxygen release with a novel planar optode system in the field. Flux data collected during the measurement period encompasses a large variation in salinity ranging from drought to record precipitation years. We developed a method to extract functional relationships from the flux data using artificial neural networks, identifying salinity thresholds for CH 4 fluxes. Finally, we are using the coupled ELM-PFLOTRAN model to test the impact of antecedent hydrological conditions on the salinity-CH 4 flux relationship. This grant contributed to the professional development of one postdoc, three research assistants and one graduate student. The sensor data has been shared with external collaborators.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Growth and biochemical composition of the carrageenophyte Eucheumatopsis isiformis (Solieriaceae, Rhodophyta) under cultivation at different irradiance levels

Long-term production of a few species of Eucheuma and Kappaphycus from the Indo-Pacific Ocean supports the global carrageenan industry. The widespread use of vegetative propagation has resulted in low genetic diversity among farmed populations, making these crops increasingly vulnerable to diseases and environmental stressors, and resulting in declining yields and reduced biomass production over time. Insufficient efforts to develop and select new native cultivars, combined with the ecological risks posed by introducing nonindigenous species, underscore the urgent need to enhance cultivar diversification in eucheumatoid farming. Eucheumatopsis isiformis , a native carrageenophyte to the Atlantic Ocean, has potential for its cultivation in the Yucatán peninsula, Mexico. This study evaluated the growth and biochemical composition (pigments, elemental C and N, kappa/iota carrageenan ratio) of E. isiformis under different irradiance conditions in an indoor cultivation system. Specimens were collected in a subtidal population in Yucatán, Mexico, characterized, acclimatized to laboratory conditions, and exposed to five irradiance levels (71 ± 10.59 –1014 ± 65.85 µmol quanta m −2 s −1 ; 23.4 °C, salinity 33–34). Growth was measured weekly for six weeks, and elemental C and N content, pigment composition, and the kappa/iota-carrageenan ratio were evaluated. Growth and chromatic photoacclimation to light conditions were observed in all treatments. Highest growth rates (3.1–3.2% day −1 ) were registered between 238 ± 28.3 – 518 ± 44.3 µmol quanta m −2 s −1 , with a predominant iota-type carrageenan (0.97 ± 0.02) observed in all treatments. This study aims to provide guidelines for the successful development of the aquaculture of this carrageenophyte.

FTIR↗
DOE OSTI2026

Hydrological and Thermal Dynamics of a Supra‐Permafrost Subterranean Estuary

Subterranean estuaries (STEs), where groundwater interacts with seawater, influence surface and subsurface coastal ecology and biogeochemistry. In Arctic-STEs overlying permafrost, groundwater flow and heat transport determine the fate of organic matter. Yet, direct observations of groundwater flow and heat and solute transport processes in Arctic STEs remain limited. This study characterized groundwater flow paths and fluxes and heat transport within an Arctic-STE along Alaska's Beaufort Sea coast during thawing, summer, and freeze-up. Intertidal seabed temperature-depth profiles collected along a 10-m transect captured the active groundwater flow period, from thaw and flow onset in mid-June to freeze-up in late-September. During this period, aquifer geometry evolved non-uniformly due to spatially varying thaw rates across the STE (mean (m) thaw depths–beach: 0.25 to 0.55–0.6 m on 20 June, 25 July, 1 October; seabed: 0.6–0.9 m from 25 July to 1 October). Groundwater and surface water levels, salinity, and subsurface temperature profiles measured over tidal time scales were interpreted alongside groundwater flow-heat transport numerical simulations. Fresh groundwater discharge was sporadic during thawing (m: 0.32 m 3 /day/m), abundant in summer (m: 0.45 m 3 /day/m), and was largely absent during freeze-up. During freeze-up, groundwater flow was driven exclusively by seawater recirculation via tidal pumping (from thawing to summer to freeze-up: 0.00025–0.15–0.5 m3/day/m) and convection. Heat advection dominated near aquatic interfaces (shaping intertidal ice), and conduction controlled vertical temperature gradients in low-flow and unsaturated sediments. These findings will help predict how prolonged summers will alter Arctic-STE cryo-hydrology and biogeochemistry.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

A family of tubular pili from harmful algal bloom forming cyanobacterium Microcystis aeruginosa

Cyanobacteria are vital photosynthetic prokaryotes, but some form harmful algal blooms (cyanoHABs) that disrupt ecosystems and produce toxins. The mechanisms by which these blooms form have yet to be fully understood, particularly the role of extracellular components. Here, we present a 2.4 Å cryo-EM structure of a pilus, termed the cyanobacterial tubular (CT) pilus, found in the cyanoHAB-forming Microcystis aeruginosa. The pilin exhibits a unique protein fold, forming a tubular pilus structure with tight, double-layer anti-parallel β-sheet interactions. We show that CT pili are essential for buoyancy by facilitating the formation of micro-colonies, which increases drag force and prevents sinking. The CT pilus surface is heavily glycosylated with ten monosaccharide modifications per pilin. Furthermore, CT pili can enrich microcystin, potentially enhancing cellular resilience, and co-localize with iron-enriched extracellular matrix components. Thus, we propose that this pilus plays an important role in the proliferation of cyanoHABs. This just discovered pilus family appears to be widely distributed across several cyanobacterial orders. Our structural and functional characterization of CT pili provide insights into cyanobacterial cell morphology, physiology, and toxin interactions, and identify potential targets for disrupting bloom formation.

Cryoelectron microscopy↗
DOE OSTI2026

Quantitative stable isotope probing (qSIP)-informed metagenomics identifies viruses infecting chemoautotrophs

Aquatic environments absorb ~2.5 gigatonnes of atmospheric carbon each year1, more than the carbon stored in the atmosphere, soils, and all biomass combined. Primary producers transform this dissolved inorganic carbon into biomass that can subsequently flow into other trophic levels, or be released back into the environment through viral lysis. While there is substantial knowledge about the diversity and activity of viruses infecting photoautotrophic primary producers and the ecosystem impact, little is known about viruses infecting chemoautotrophs, representing a gap in our understanding of key processes driving microbial carbon cycling. Here, we combine metagenomics with quantitative 12/13C stable isotopic probing (qSIP) mesocosm experiments in a marine-derived meromictic pond to quantify population-specific isotopic enrichment, identify key chemoautotrophic primary producers, and virus-host dynamics. Isotopically enriched carbon is tracked from the genomes of chemoautotrophs to putative viruses, showing that active populations of hydrogen/sulfur-oxidizing chemoautotrophs (Thiomicrorhabdus, Hydrogenovibrio, Sulfurimonas, Sulfurovum) are targeted by viruses. This work provides the foundation for revealing the diversity and activity of viruses infecting globally-widespread chemoautotrophs. Our study sheds light on trophic interactions that impact microbial carbon cycling in aphotic environments and builds toward biogeochemical models that incorporate viral impacts on chemoautotrophic microbial communities.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

The hidden influence of terrestrial groundwater on salt marsh function and resilience

Salt marshes are hotspots of nutrient processing and carbon sequestration. So far, studies addressing spatiotemporal variability in and drivers of salt marsh biogeochemical function, carbon storage and resilience have focused on ocean-driven surface hydrologic influences, neglecting effects of terrestrial hydrology through subsurface connections. Here we evaluate drivers of salt marsh redox potential, a proxy for biogeochemical state, through wavelet analyses and information theory using data from seven marshes. The results point to terrestrial groundwater level as a dominant control on redox variability across all sites. Because redox is a key driver of biogeochemical processes, and specifically oxidation of organic matter that sequesters carbon and maintains marsh elevation, these terrestrial influences are critical to understanding marsh function and evolution. Further, the newly identified links between onshore groundwater levels and marsh redox conditions shift the traditional paradigm and suggest that terrestrial hydrology is a primary control on salt marsh carbon sequestration potential and resilience.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Identification of key steps in the evolution of anaerobic methanotrophy in Candidatus Methanovorans (ANME-3) archaea

Despite their large environmental impact and multiple independent emergences, the processes leading to the evolution of anaerobic methanotrophic archaea (ANME) remain unclear. This work uses comparative metagenomics of a recently evolved but understudied ANME group, “Candidatus Methanovorans” (ANME-3), to identify evolutionary processes and innovations at work in ANME, which may be obscured in earlier evolved lineages. We identified horizontal transfer of hdrA homologs and convergent evolution in carbon and energy metabolic genes as potential early steps in Methanovorans evolution. We also identified the erosion of genes required for methylotrophic methanogenesis along with horizontal acquisition of multiheme cytochromes and other loci uniquely associated with ANME. The assembly and comparative analysis of multiple Methanovorans genomes offers important functional context for understanding the niche-defining metabolic differences between methane-oxidizing ANME and their methanogen relatives. Furthermore, this work illustrates the multiple evolutionary modes at play in the transition to a globally important metabolic niche.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Redox potential in Typha-dominated tidal brackish marsh, PIE LTER, Plum Island Sound, MA, June–December 2022

This dataset includes soil redox potential measurements collected at multiple depths within a tidal brackish wetland in the upper estuary of the Plum Island Ecosystems Long-Term Ecological Research site (PIE LTER), Plum Island Sound, Newbury, Massachusetts. Measurements were taken to evaluate temporal variation in redox potential in relation to hydrological events at three replicate locations. Data were recorded every 5 minutes using a Campbell Scientific Volt116 connected to a CR6 datalogger with SWAP instrument redox probes (ORP-30-4-B) and reference electrodes. Measurements were made at the AmeriFlux site US-PLo at four soil depths (5, 10, 15, and 30 cm). The file redox_soiltemp_2022.csv contains temperature-corrected redox values and soil temperature following Silva-Machado et al. (2024). Metadata files redox_soiltemp_dd.csv and redox_soiltemp_flmd.csv provide detailed descriptions of variables and site locations.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Specific conductivity and salinity of the Parker River, PIE LTER, Plum Island Sound MA, August-November 2022

This dataset contains specific conductivity and calculated salinity data of Parker River water at a tidal brackish wetland dominated by Typha angustifolia at the upper estuary of the Plum Island Sound in Newbury, Massachusetts (MA) within the Plum Island Ecosystems Long Term Ecological Research site (PIE LTER). Measurements were taken to evaluate temporal changes in surface water salinity in high frequency to characterize boundary conditions of soil and plant responses to changes in salinity. A PVC pipe was installed in a low elevation spot in the creek bank so that the bottom of the pipe sat on the sediment surface allowing flushing with water during flooding. Raw measurements were collected using an Onset HOBO U24-002 Saltwater Conductivity/Salinity data logger. The specific conductance and salinity measurements were corrected and calculated respectively using Onset’s HOBOware software and reference specific conductivity measurements taken in tandem with the first and last points recorded by the HOBO sensor. These reference measurements were taken using a HACH HQ14D Portable Conductivity Meter. Because of the installation design, only data one hour before and after high tide are used. Metadata files Typha_ctd_salinity_dd.csv and Typha_ctd_salinity_flmd.csv contain detailed information on data variables, sampling and QA/QC methods, and site location.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Redox potential in Typha-dominated tidal brackish marsh, PIE LTER, Plum Island Sound, MA, 2023

This dataset includes soil redox potential measurements collected at multiple depths within a tidal brackish wetland in the upper estuary of the Plum Island Ecosystems Long-Term Ecological Research site (PIE LTER), Plum Island Sound, Newbury, Massachusetts (MA). Measurements were taken to evaluate temporal variation in redox potential in relation to hydrological events at three replicate locations. Data were recorded every 5 minutes using a Campbell Scientific Volt116 connected to a CR6 datalogger with SWAP instrument redox probes (ORP-30-4-B) and reference electrodes. Measurements were made at the AmeriFlux site US-PLo at four soil depths (5, 10, 15, and 30 cm). The file redox_soiltemp_2023.csv contains temperature-corrected redox values and soil temperature following Silva-Machado et al. (2024). Metadata files redox_soiltemp_dd.csv and redox_soiltemp_2023_flmd.csv provide detailed descriptions of variables and site locations.

54 ENVIRONMENTAL SCIENCES↗