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At least 19 records

Isolation Standard Measures: A Set of Validated and Feasible Measurements Ensuring Comparability Across Isolation and Confinement Studies

Isolation and confinement studies have been essential to the preparation of crewed long-duration space missions, acting as analogues that facilitate the study of psychological and physiological responses to isolation and confinement. They also serve as opportunities for the development, testing, and validation of countermeasures and coping methods to handle the challenges that arise in such scenarios. Due to the small sample sizes in combination with high inter-individual variability, single isolation campaigns are not always sufficient for obtaining statistically significant scientific findings. To address this issue and improve comparability between different studies, the need for standardized measures to be collected in all future isolation and confinement studies was identified. Additionally, these measures should also be shown to be generally valid, reliable, feasible and acceptable in analogue and spaceflight environments. Standard measures in isolation and confinement studies allow for more direct comparisons of results and synthesis of data across isolation and confinement studies as well as provide an important step toward standard measures in spaceflight. An international expert group with representatives from different space agencies worldwide was brought together to define a core set of standard measures for isolation and confinement studies. This paper provides an overview of the expert group’s recommendations for international standard measures for future isolation and confinement studies, along with subsequent updates coordinated by the International Countermeasures Working Group (ICMWG), which was established as a sub-Working Group under the International Space Life Sciences Working Group (ISLSWG). Additional experts were consulted by the ICMWG partner agencies as required. The collection of the described set of isolation standard measures will provide data on the following parameters: sleep, mood, psychological state, psychophysiology, cognitive performance, stress and the immune system, general health and well-being, team measures, nutritional measures, and environmental conditions. For each measure, recommendations were made about duration and frequency of administration, along with specific implementation recommendations in relation to the duration of the isolation study. The set of isolation standard measures will be reassessed every two years at a minimum to ensure they are up to date and reflect the current state-of-the-art.

Isolation

Ground Isolation Circuit for Isolating a Transmission Line from Ground Interference

This invention relates generally to a system for isolating ground interference from a transmission line, e.g., a ground isolation circuit for isolating a wideband transmission signal (such as a video signal) from ground by modulating the base signal on a carrier signal to permit the transmission signal to be isolated. In one embodiment, the circuit includes a pair of matched mixer circuits, each of which receives a carrier signal from the same oscillator circuit. The first mixer circuit also receives the baseband signal input, after appropriate conditioning, and modulates the baseband signal onto the carrier signal. In a preferred embodiment the carrier signal has a predetermined frequency which is at least two times the frequency of the baseband signal. The modulated signal (which can comprise an rf signal) is transmitted via an rf transmission line to the second mixer, which demodulates the rf signal to recover the baseband signal. Each port of the mixer connects to an isolation transformer to ensure isolation from ground interference. The circuit is considered to be of commercial value in that it can provide isolation between transmitting and receiving circuits, e.g., ground isolation for television circuits or high frequency transmitters, without the need for video transformers or optical isolators, thereby reducing the complexity, power consumption, and weight of the system.

Davidson, Craig A.

HtPIP: High-throughput phage isolation platform increases diversity and reduces isolation time using multiple bacteria

Bacteriophages are ubiquitous in nature, but relatively few have been isolated and characterized compared to the number of bacterial strains. Phage biotechnology applications benefit from a diverse library of isolated phages to kill or transfer genetic material to a bacterium of interest. However, scaling up phage discovery for diverse bacterial hosts can be time-consuming and costly. Here, we developed an approach to capture novel phages for multiple bacterial strains in parallel from an environmental sample using commercially available 0.2-μM filter plates. Using this High-throughput Phage Isolation Platform (HtPIP), 12 novel phages were isolated spanning 9 diverse bacterial host genera. Eleven of the isolated phages define new phage species, with nine also defining new genera. The HtPIP was used to discover both DNA and RNA phages, including a Tectiviridae infecting Pseudomonas putida mt-2 and a Leviviricetes infecting a Microbacterium isolate, which represents the first cultured RNA phage infecting a host outside of Proteobacteria. Using a metagenomic approach, we demonstrate that the HtPIP captures a higher proportion of novel phages compared to traditional low-throughput methods.

High-throughput

Low-loss, high-isolation, fiber-optic isolator

A low-loss, high-isolation, fiber-optic isolator for use in single-mode fiber systems utilizes a Faraday rotator and two polarizers, one at each end angularly oriented from each other at the angle of rotation for isolation, and two aspheric lens connectors to couple optical fibers to the Faraday isolator to reduce forward loss to about 2.5 dB and improve isolation to greater than 70 dB.

Lutes, George F.

Ground Isolation Circuit for Isolating a Transmission Line from Ground Interference

An isolation circuit is disclosed for isolating ground interference from a wideband transmission signal. The ground isolation circuit of the present invention is constructed using a pair of matched mixer circuits. each of which receives a carrier signal from the same oscillator circuit. The first mixer circuit also receives the baseband signal input after appropriate conditioning, and modulates the baseband signal onto the carrier signal. In the preferred embodiment, the carrier signal has a predetermined frequency which is at least two times the frequency of the baseband signal. The modulated signal (which preferably comprises an rf signal) is transmitted via an rf transmission line to the second mixer, which demodulates the rf signal to recover the baseband signal. Each port of the mixer circuits connects to an isolation transformer to insure isolation from ground interference.

Davidson, Craig A.

A Novel Three-Phase Isolated LLC and Non-Isolated LCL-T Resonant Converter for Fuel Cell Applications

In this paper, a novel three-phase isolated LLC and non-isolated LCL−T resonant converter topologies are introduced for fuel cell applications. In order to improve the fuel cell DC/DC converter efficiency, the current amplitude should be reduced in the power stage components. Cascaded connections of fuel cell blocks through a controllable system enable using higher voltage amplitude and bring the current amplitude lower at the target power. In this way, power losses in the passive components can be reduced, and maximum energy transfer can be established, improving the DC/DC converter efficiency from the fuel cell to the load. The introduced new converter also achieves soft switching (ZVS), minimizing the switching losses in all input and output load conditions. The presented three-phase isolated LLC and non-isolated LCL−T resonant converter systems, fed by three fuel cell modules with an output range of 190380 V, deliver 580−730 V at 450 kW maximum output power. The results reveal that the proposed systems have the advantage of reducing the size, volume, and weight and increasing the overall DC/DC converter system efficiency compared to the single-phase systems.

Asa, Erdem [ORNL] (ORCID:0000000190884812)

Isolated resonator gyroscope with isolation trimming using a secondary element

The present invention discloses a resonator gyroscope including an isolated resonator. One or more flexures support the isolated resonator and a baseplate is affixed to the resonator by the flexures. Drive and sense elements are affixed to the baseplate and used to excite the resonator and sense movement of the gyroscope. In addition, at least one secondary element (e.g., another electrode) is affixed to the baseplate and used for trimming isolation of the resonator. The resonator operates such that it transfers substantially no net momentum to the baseplate when the resonator is excited. Typically, the isolated resonator comprises a proof mass and a counterbalancing plate.

Challoner, A. Dorian

Direct Exploration of the L2 Isolated Invariant Set Using Isolating Neighborhoods

Previous studies have used isolating blocks to explore the isolated invariant set around the L2 Lagrange point in the circular restricted three-body problem by computing trajectories on the asymptotic set and using them to track particular orbits. Once an initial map of the location of the orbits within the isolated invariant set projected into configuration space has been found, it is possible to further explore these orbits more directly by starting from appropriately chosen points within the mapped space. Here, we develop a method that uses these points to explore the periodic and quasiperiodic orbits within the isolated invariant set.

Lo, Martin W.

Model-based fault detection and isolation for intermittently active faults with application to motion-based thruster fault detection and isolation for spacecraft

The present invention is a method for detecting and isolating fault modes in a system having a model describing its behavior and regularly sampled measurements. The models are used to calculate past and present deviations from measurements that would result with no faults present, as well as with one or more potential fault modes present. Algorithms that calculate and store these deviations, along with memory of when said faults, if present, would have an effect on the said actual measurements, are used to detect when a fault is present. Related algorithms are used to exonerate false fault modes and finally to isolate the true fault mode. This invention is presented with application to detection and isolation of thruster faults for a thruster-controlled spacecraft. As a supporting aspect of the invention, a novel, effective, and efficient filtering method for estimating the derivative of a noisy signal is presented.

Wilson, Edward

Contrasting effects of glutamate and branched-chain amino acid metabolism on acid tolerance in a Castellaniella isolate from acidic groundwater

Groundwater acidification co-occurring with nitrate pollution is a common, global environmental health hazard. Denitrifying bacteria have been leveraged for the in situ removal of nitrate in groundwater. However, co-existing stressors—such as low pH—reduce the efficacy of biological removal processes. Castellaniella sp. str. MT123 is a complete denitrifier that was isolated from acidic, nitrate-contaminated groundwater. The strain grows robustly by nitrate respiration at pH < 6.0, completely reducing nitrate to dinitrogen gas. Genomic analyses of MT123 revealed few previously characterized acid tolerance genes. Thus, we utilized a combination of proteomics, metabolomics, and competitive mutant fitness to characterize the genetic mechanisms of MT123 acclimation to growth under mildly acidic conditions. We found that glutamate accumulation is critical in the acid acclimation of MT123, possibly through consumption of intracellular protons via glutamate decarboxylation to GABA. This is despite the fact that MT123 lacks the canonical glutamate decarboxylase-glutamate/GABA antiporter system implicated in acid tolerance in other bacteria. In contrast, branched-chain amino acid (BCAA) accumulation was detrimental to cell growth at lower pHs, possibly through indirect mechanisms impacting the cellular glutamate pool. Genetic analysis previously linked MT123 to a population of Castellaniella that bloomed—concurrent to nitrate removal—during a biostimulation effort to reduce groundwater nitrate concentrations at MT123’s location of origin. Thus, our analyses provide novel insight into mechanisms of acclimation to acidic conditions in a strain with significant potential for nitrate bioremediation.

59 BASIC BIOLOGICAL SCIENCES

Synthesis of a system with active vibration isolation, considering the vibroacoustical characteristics of the source and of the isolated object

The efficiency and resistance of an active vibration isolation system (AVI) are analyzed for the case of unidirectional vibrations of a mass on a spring, with a damper, resting on a rigid base. The effect of certain vibroacoustical characteristics of real objects on AVI resistance and synthesis of AVI, which are effective over a wide frequency band are considered. A numerical analysis of the response of a mechanical system with AVI features is presented.

Genkin, M. D.

STS-43 Atlantis/Breakfast & Suit-Up, Depart O&C, Ingress, Launch with Isolated Views, TDRS-E Deploy, and Landing with Isolated Views

Footage of various stages of the STS-43 Atlantis launch is shown, including shots of the crew at breakfast, getting suited up, and departing to board the Orbiter. The launch is shown from many vantage points, as is the landing. Atlantis is shown from space and the deployment of the fifth Tracking and Data Relay Satellite (TDRS-E) is also shown.

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