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Probabilistic Mixture Model-Based Spectral Unmixing

Spectral unmixing attempts to decompose a spectral ensemble into the constituent pure spectral signatures (called endmembers) along with the proportion of each endmember. This is essential for techniques like hyperspectral imaging (HSI) used in environment monitoring, geological exploration, etc. Several spectral unmixing approaches have been proposed, many of which are connected to hyperspectral imaging. However, most extant approaches assume highly diverse collections of mixtures and extremely low-loss spectroscopic measurements. Additionally, current non-Bayesian frameworks do not incorporate the uncertainty inherent in unmixing. We propose a probabilistic inference algorithm that explicitly incorporates noise and uncertainty, enabling us to unmix endmembers in collections of mixtures with limited diversity. We use a Bayesian mixture model to jointly extract endmember spectra and mixing parameters while explicitly modeling observation noise and the resulting inference uncertainties. We obtain approximate distributions over endmember coordinates for each set of observed spectra while remaining robust to inference biases from the lack of pure observations and the presence of non-isotropic Gaussian noise. As a direct impact of our methodology, access to reliable uncertainties on the unmixing solutions would enable robust solutions to noise, as well as informed decision-making for HSI applications and other unmixing problems.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

HAMscope: a snapshot Hyperspectral Autofluorescence Miniscope for real-time molecular imaging

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield miniscope, HAMscope spectrally encodes each frame and employs a probabilistic deep learning framework to reconstruct 30-channel hyperspectral stacks (452-703 nm) or directly infer molecular composition maps from single images. A scalable multi-pass U-Net architecture with transformer-based attention and per pixel uncertainty estimation enables high spatio-spectral fidelity (mean absolute error ∼0.0048) at video rates. While initially demonstrated in plant systems, including lignin, chlorophyll, and suberin imaging in intact poplar and cork tissues, the platform is readily adaptable to other applications such as neural activity mapping, metabolic profiling, and histopathology. We show that the system generalizes to out-of-distribution tissue types and supports direct molecular mapping without the need for spectral unmixing. HAMscope establishes a general framework for compact, uncertainty-aware spectral imaging that combines minimal optics with advanced deep learning, offering broad utility for real-time biochemical imaging across neuroscience, environmental monitoring, and biomedicine.

59 BASIC BIOLOGICAL SCIENCES↗