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At least 19 records

Rapid in situ hybridization technique using 16S rRNA segments for detecting and differentiating the closely related gram-positive organisms Bacillus polymyxa and Bacillus macerans

A rapid, sensitive, inexpensive in situ hybridization technique, using 30-mer 16S rRNA probes, can specifically differentiate two closely related Bacillus spp., B. polymyxa and B. macerans. The 16S rRNA probes were labeled with a rhodamine derivative (Texas Red), and quantitative fluorescence measurements were made on individual bacterial cells. The microscopic fields analyzed were selected by phase-contrast microscopy, and the fluorescence imaging analyses were performed on 16 to 67 individual cells. The labeled 16S rRNA probe, POL, whose sequence was a 100% match with B. polymyxa 16S rRNA but only a 60% match with B. macerans 16S rRNA, gave quantitative fluorescence ratio measurements that were 34.8-fold higher for B. polymyxa cells than for B. macerans cells. Conversely, the labeled probe, MAC, which matched B. polymyxa 16S rRNA in 86.6% of its positions and B. macerans 16S rRNA in 100% of its positions, gave quantitative fluorescence measurements that were 59.3-fold higher in B. macerans cells than in B. polymyxa cells. Control probes, whose 16S rRNA sequence segment (P-M) was present in both B. polymyxa and B. macerans as well as a panprokaryotic probe (16S), having a 100% match with all known bacteria, hybridized equally well with both organisms. These latter hybridizations generated very high fluorescence signals, but their comparative fluorescence ratios (the differences between two organisms) were low. The control paneukaryotic probe (28S), which had less than 30% identity for both B. macerans and B. polymyxa, did not hybridize with either organism.

Non-NASA Center↗

A hybrid technique for wire antennas in a cavity

An evaluation is conducted of two numerical techniques developed by Richmond (1961, 1966) for wire antennas and scattering bodies. It is concluded that a more efficient and accurate scheme can be devised by suitably combining the two techniques. This scheme, which is called the 'hybrid method', is well suited for a wire antenna mounted in a cavity. Reaction matching is employed in the case of the antenna, while point matching is used for the support structure. The described approach makes it possible to consider voltage generators without the use of any equivalent magnetic frill current sources. The number of unknown currents to be determined is essentially optimized by this combination of reaction matching and point matching. The resulting current distribution is a quantized distribution on the support structure and is continuous on the main radiator.

Agrawal, P. K.↗

Hybrid techniques for complex aerospace electromagnetics problems

Important aerospace electromagnetics problems include the evaluation of antenna performance on aircraft and the prediction and control of the aircraft's electromagnetic signature. Due to the ever increasing complexity and expense of aircraft design, aerospace engineers have become increasingly dependent on computer solutions. Traditionally, computational electromagnetics (CEM) has relied primarily on four disparate techniques: the method of moments (MoM), the finite-difference time-domain (FDTD) technique, the finite element method (FEM), and high frequency asymptotic techniques (HFAT) such as ray tracing. Each of these techniques has distinct advantages and disadvantages, and no single technique is capable of accurately solving all problems of interest on computers that are available now or will be available in the foreseeable future. As a result, new approaches that overcome the deficiencies of traditional techniques are beginning to attract a great deal of interest in the CEM community. Among these new approaches are hybrid methods which combine two or more of these techniques into a coherent model. During the ASEE Summer Faculty Fellowship Program a hybrid FEM/MoM computer code was developed and applied to a geometry containing features found on many modern aircraft.

Aberle, Jim↗

Hidden-line removal at 20 pictures/second through hybrid techniques.

A real-time computer image generation method is presented that is designed to operate in a parallel-computing environment. It can provide good quality pictures of limited complexity if implemented on a general purpose hybrid computing system. Such a hybrid approach can generate a new image every 50 milliseconds. These results have been demonstrated by a feasibility test program which impelements the hybrid concepts described. This hybrid-picture generation capability is shown to represent a significant contribution to the area of real-time hidden-line removal.

Brown, R. M.↗

A hybrid technique for spacecraft attitude interpolation with arbitrary attitude data gaps

An algorithm for obtaining sufficiently accurate estimates of a system parameter Y, given a data record of the parameter in which at arbitrary times no direct observations of Y exist, and describing the variance of these estimates, is proposed. The method provides estimates at all requested times, and the variance of the estimates is maintained as close to the variance of directly measured data as the quality of input information will allow. Three classes of information are used: (1) the measured data record of Y and the statistical extension of this record into time intervals of no direct measurement, obtained by a modification of standard autocorrelation techniques; (2) an a priori indicator of the behavior of Y; and (3) one or more indirect indicators of Y based on observation of other system parameters. The application of the algorithm to the SEASAT-A attitude determination under conditions of sensor field-of-view limitations and sun occultations is presented.

Treder, A. J.↗

Fast Computation of Frequency Response of Cavity-Backed Apertures Using MBPE in Conjunction with Hybrid FEM/MoM Technique

The hybrid Finite Element Method(FEM)/Method of Moments(MoM) technique has become popular over the last few years due to its flexibility to handle arbitrarily shaped objects with complex materials. One of the disadvantages of this technique, however, is the computational cost involved in obtaining solutions over a frequency range as computations are repeated for each frequency. In this paper, the application of Model Based Parameter Estimation (MBPE) method[1] with the hybrid FEM/MoM technique is presented for fast computation of frequency response of cavity-backed apertures[2,3]. In MBPE, the electric field is expanded in a rational function of two polynomials. The coefficients of the rational function are obtained using the frequency-derivatives of the integro-differential equation formed by the hybrid FEM/MoM technique. Using the rational function approximation, the electric field is calculated at different frequencies from which the frequency response is obtained.

Reddy, C. J.↗

Hybrid analytical technique for the nonlinear analysis of curved beams

The application of a two-step hybrid technique to the geometrically nonlinear analysis of curved beams is used to demonstrate the potential of hybrid analytical techniques in nonlinear structural mechanics. The hybrid technique is based on successive use of the perturbation method and a classical direct variational procedure. The functions associated with the various-order terms in the perturbation expansion of the fundamental unknowns, and their sensitivity derivatives with respect to material and geometric parameters of the beam, are first obtained by using the perturbation method. These functions are selected as coordinate functions (or modes) and the classical direct variational technique is then used to compute their amplitudes. The potential of the proposed hybrid technique for nonlinear analysis of structures is discussed. The effectiveness of the hybrid technique is demonstrated by means of numerical examples. The symbolic computation system Mathematica is used in the present study. The tasks performed on Mathematica include: (1) generation of algebraic expressions for the perturbation functions of the different response quantities and their sensitivity derivatives: and (2) determination of the radius of convergence of the perturbation series.

Noor, A. K.↗

Hybrid perturbation/Bubnov-Galerkin technique for nonlinear thermal analysis

A two step hybrid analysis technique to predict the nonlinear steady state temperature distribution in structures and solids is presented. The technique is based on the regular perturbation expansion and the classical Bubnov-Galerkin approximation. The functions are obtained by using the regular perturbation method. These functions are selected as coordinate functions and the classical Bubnov-Galerkin technique is used to compute their amplitudes. The potential of the proposed hybrid technique for the solution of nonlinear thermal problems is discussed. The effectiveness of this technique is demonstrated by the effects of conduction, convection, and radiation modes of heat transfer. It is indicated that the hybrid technique overcomes the two major drawbacks of the classical techniques: (1) the requirement of using a small parameter in the regular perturbation method; and (2) the arbitrariness in the choice of the coordinate functions in the Bubnov-Galerkin technique. The proposed technique extends the range of applicability of the regular perturbation method and enhances the effectiveness of the Bubnov-Galerkin technique.

Noor, A. K.↗

Radiation Characteristics of Cavity Backed Aperture Antennas in Finite Ground Plane Using the Hybrid FEM/MoM Technique and Geometrical Theory of Diffraction

A technique using hybrid Finite Element Method (FEM)/Method of Moments (MoM), and Geometrical Theory of Diffraction (GTD) is presented to analyze the radiation characteristics of cavity fed aperture antennas in a finite ground plane. The cavity which excites the aperture is assumed to be fed by a cylindrical transmission line. The electromagnetic (EM) fields inside the cavity are obtained using FEM. The EM fields and their normal derivatives required for FEM solution are obtained using (1) the modal expansion in the feed region and (2) the MoM for the radiating aperture region(assuming an infinite ground plane). The finiteness of the ground plane is taken into account using GTD. The input admittance of open ended circular, rectangular, and coaxial line radiating into free space through an infinite ground plane are computed and compared with earlier published results. Radiation characteristics of a coaxial cavity fed circular aperture in a finite rectangular ground plane are verified with experimental results.

Reddy, C. J.↗

An improved data transfer and storage technique for hybrid computation

Improved technique was developed for transferring and storing data at faster than real time speeds on hybrid computer. Predominant advantage is combined use of electronic relays, track and store units, and analog-to-digital and digital-to-analog conversion units of hybrid computer.

Hansing, A. M.↗

A Novel Hybrid Ultramicrotomy/FIB-SEM Technique: Preparation of Serial Electron-Transparent Thin Sections of a Hayabusa Grain

The Japanese space agency's (JAXA) Hayabusa mission returned the first particulate samples (typically <100micron) from the surface of an asteroid (25143 Itokawa). These precious samples provide important insights into early Solar System processes, but their sizes pose tremendous challenges to coordinated analysis using a variety of nano- and micro-beam techniques. The ability to glean maximal information from individual particles has become increasingly important and depends critically on sample preparation. We developed a hybrid technique combining traditional ultramicrotomy with focused ion beam (FIB) techniques, allowing for more thorough in situ investigations of grain surfaces and interiors. Using this method, we increase the number of FIB-prepared sections that can be recovered from a particle with dimensions on the order of tens of microns. These sections can be subsequently analyzed using a variety of analytical techniques. Particle RA-QD02-0211 is a approx. 40×40×20 micron particle from Itokawa containing olivine and Fe sulfides. It was embedded in low viscosity epoxy and partly sectioned to a depth of approx 10 micron; sections are placed on Cu grids with thin amorphous films for transmission electron microscope (TEM) analyses. With the sample surface partly exposed, the epoxy bullet is trimmed to a height of approx. 5mm to accommodate the allowable dimensions for FIB work (FEI Quanta 600 3D dual beam FIB-SEM). Using a diamond trim knife, the epoxy surrounding the grain is removed on 3 sides (to within a few microns of the grain); the depth of material removed extends well below the bottom of the particle. The sample is attached to an SEM pin mount, the epoxy coated with conductive paint, and the entire assembly coated with approx. 40nm of carbon to eliminate sample charging during FIB work. A protective carbon cap is placed according to the plan for the 15 FIB sections. The central 'spine' of the cap runs perpendicular to the front of the sample, and the 'ribs' protruding from either side run parallel. Each rib indicates the location of a planned FIB section, and the spine contains the final two planned sections. We use a cap with a 4 micron-wide spine and 2micron-wide ribs that have ≳3.5 micron of space between them (narrower cuts result in too much re-deposition of material inside the trenches). Using a 30kV, 3nA ion-beam we expose the front surface of the grain and commence milling trenches between sections. Rather than using the typical C-cut to prepare the sample for lift-out, an L-cut is used instead, leaving the sample connected by an interior tab. tab. Sections are lifted out, attached to TEM grids and thinned to electron transparency. TEM analyses show that our hybrid technique preserves both interior and edge features, including surface modifications from exposure to the space environment, such as damaged rims that form in response to solar wind implantation effects and adhering grains. In addition, the FIB sections provide larger areas that are free of fractures and chatter effects in comparison to the microtome thin sections, thus enabling more accurate measurements of solar flare particle track densities that are used to determine the surface exposure age of the particles.

Berger, Eve L.↗

Hybrid Analytical Technique for Nonlinear Vibration Analysis of Thin-Walled Beams

A two-step hybrid analytical technique is presented for the nonlinear vibration analysis of thin-walled beams. The first step involves the generation of various-order perturbation functions using the Linstedt-Poincare perturbation technique. The second step consists of using the perturbation functions as coordinate (or approximation) functions and then computing both the amplitudes of these functions and the nonlinear frequency of vibration via a direct variational procedure. The analytical formulation is based on a form of the geometrically nonlinear beam theory with the effects of in-plane inertia, rotatory inertia, and transverse shear deformation included. The effectiveness of the proposed technique is demonstrated by means of a numerical example of thin-walled beam with a doubly symmetric I-section. The solutions obtained using a single-spatial mode were compared with those obtained using multiple-spatial modes. The standard of comparison was taken to be the frequencies obtained by the direct integration/fast Fourier transform (FFT) technique. The nonlinear frequencies obtained by the hybrid technique were shown to converge to the corresponding ones obtained by the direct integration/fast Fourier transform (FFT) technique well beyond the range of applicability of the perturbation technique. The frequencies and total strain energy of the beam were overestimated by using a single-spatial mode.

Noor, Ahmed K.↗

A Hybrid SBR/MoM Technique for Analysis of Scattering from Small Protrusions on a Large Conducting Body

For analysis of large-scale electromagnetic scattering problems, high-frequency asymptotic methods are fast but approximate, whereas low-frequency numerical methods are accurate but slow. Neither can produce an efficient and accurate solution to scattering by large bodies containing small structures. A promising approach is to combine the best features of both types of methods to produce a hybrid technique that is sufficiently fast, reasonably accurate, and applicable to a class of unsolvable problems such as the scatterers mentioned above. There are two extremes for this type of hybridization. One is simply to superimpose solutions from asymptotic and numerical methods. While this approach is most widely used in practical applications, it neglects the interactions between the two solutions, which can be significant in many problems. The other extreme is to combine an asymptotic and a numerical method in an exact manner. In this approach, the effect of a large body is included by incorporating its diffraction into the Green's function in the integral equation for the small structures, which accounts for all interactions. While this approach is accurate, it is difficult to be implemented in a general-purpose computer code because of its complex nature. A more practical approach is to develop a technique that can include all significant interactions and neglect all trivial interactions. The resulting hybrid technique can produce sufficient accuracy and can be implemented in a general-purpose computer code. In this paper, we develop a technique that combines the shooting and- bouncing-ray (SBR) method and the method of moments (MoM) to solve for the scattering by large conducting bodies with small structures mounted on their surfaces.

Jin, J. M.↗

D-region blunt probe data analysis using hybrid computer techniques

The feasibility of performing data reduction techniques with a hybrid computer was studied. The data was obtained from the flight of a parachute born probe through the D-region of the ionosphere. A presentation of the theory of blunt probe operation is included with emphasis on the equations necessary to perform the analysis. This is followed by a discussion of computer program development. Included in this discussion is a comparison of computer and hand reduction results for the blunt probe launched on 31 January 1972. The comparison showed that it was both feasible and desirable to use the computer for data reduction. The results of computer data reduction performed on flight data acquired from five blunt probes are also presented.

Burkhard, W. J.↗

Minimum deltaV Burn Planning for the International Space Station Using a Hybrid Optimization Technique, Level 1

The International Space Station's (ISS) trajectory is coordinated and executed by the Trajectory Operations and Planning (TOPO) group at NASA's Johnson Space Center. TOPO group personnel routinely generate look-ahead trajectories for the ISS that incorporate translation burns needed to maintain its orbit over the next three to twelve months. The burns are modeled as in-plane, horizontal burns, and must meet operational trajectory constraints imposed by both NASA and the Russian Space Agency. In generating these trajectories, TOPO personnel must determine the number of burns to model, each burn's Time of Ignition (TIG), and magnitude (i.e. deltaV) that meet these constraints. The current process for targeting these burns is manually intensive, and does not take advantage of more modern techniques that can reduce the workload needed to find feasible burn solutions, i.e. solutions that simply meet the constraints, or provide optimal burn solutions that minimize the total DeltaV while simultaneously meeting the constraints. A two-level, hybrid optimization technique is proposed to find both feasible and globally optimal burn solutions for ISS trajectory planning. For optimal solutions, the technique breaks the optimization problem into two distinct sub-problems, one for choosing the optimal number of burns and each burn's optimal TIG, and the other for computing the minimum total deltaV burn solution that satisfies the trajectory constraints. Each of the two aforementioned levels uses a different optimization algorithm to solve one of the sub-problems, giving rise to a hybrid technique. Level 2, or the outer level, uses a genetic algorithm to select the number of burns and each burn's TIG. Level 1, or the inner level, uses the burn TIGs from Level 2 in a sequential quadratic programming (SQP) algorithm to compute a minimum total deltaV burn solution subject to the trajectory constraints. The total deltaV from Level 1 is then used as a fitness function by the genetic algorithm in Level 2 to select the number of burns and their TIGs for the next generation. In this manner, the two levels solve their respective sub-problems separately but collaboratively until a burn solution is found that globally minimizes the deltaV across the entire trajectory. Feasible solutions can also be found by simply using the SQP algorithm in Level 1 with a zero cost function. This paper discusses the formulation of the Level 1 sub-problem and the development of a prototype software tool to solve it. The Level 2 sub-problem will be discussed in a future work. Following the Level 1 formulation and solution, several look-ahead trajectory examples for the ISS are explored. In each case, the burn targeting results using the current process are compared against a feasible solution found using Level 1 in the proposed technique. Level 1 is then used to find a minimum deltaV solution given the fixed number of burns and burn TIGs. The optimal solution is compared with the previously found feasible solution to determine the deltaV (and therefore propellant) savings. The proposed technique seeks to both improve the current process for targeting ISS burns, and to add the capability to optimize ISS burns in a novel fashion. The optimal solutions found using this technique can potentially save hundreds of kilograms of propellant over the course of the ISS mission compared to feasible solutions alone. While the software tool being developed to implement this technique is specific to ISS, the concept is extensible to other long-duration, central-body orbiting missions that must perform orbit maintenance burns to meet operational trajectory constraints.

Brown, Aaron J.↗