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At least 19 records

Cotranscriptional 3′-End Processing of T7 RNA Polymerase Transcripts by a Smaller HDV Ribozyme

In vitro run-off transcription by T7 RNA polymerase generates heterogeneous 3'-ends because the enzyme tends to add untemplated adenylates. To generate homogeneous 3'-termini, HDV ribozymes have been used widely. Their sequences are added to the 3'-terminus such that co-transcriptional self-cleavage generates homogeneous 3'-ends. A shorter HDV sequence that cleaves itself efficiently would be advantageous. Here we show that a recently discovered, small HDV ribozyme is a good alternative to the previously used HDV ribozyme. The new HDV ribozyme is more efficient in some sequence contexts, and less efficient in other sequence contexts than the previously used HDV ribozyme. The smaller size makes the new HDV ribozyme a good alternative for transcript 3'-end processing.

Arvin Akoopie↗

AAM Project: High Density Vertiplex (HDV) Research and Capabilities

NASA is currently engaged in dedicated research into vertiports through its Advanced Air Mobility Project, housed within the Airspace Operations Safety Program (AOSP), through a Sub-Project referred to as High Density Vertiplex, or simply HDV. This effort’s mission is to develop a reference automation architecture for vertiports that will enable operations to scale to the density levels envisioned for future environments. Through the development of the HDV Concept of Operations and accompanying architecture documents a research roadmap was developed as part of the effort's formulation process that laid the foundations for the work and schedule to be completed as part of the HDV scope and technical challenge. This presentation provides the background of the HDV Sub-Project and outlines the concept and capabilities as it has developed to date. Summaries of the completed work package and status of current work package will be provided with details on specific capabilities and supporting integration developments.

vertiport↗

Durability of Highly Active PGM Catalyst MEA Tested Via Nitrogen and Air AST Cycling Under HDV Condition

PEMFCs are widely considered as the most promising power sources, particularly for heavy-duty vehicles (HDVs). Unfortunately, the degradation of MEAs under HDV condition remains insufficiently studied. In this work, we systematically investigated two MEAs with catalysts of Pt nanoparticles (NPs) supported over high surface area carbon black. These MEAs were tested for durability under HDV condition in nitrogen using a DOE AST protocol for 180,000 cycles, which is equivalent to 30,000 hours or 1 million miles of operation. The commercial Catalyst MEA also underwent 6,000 AST cycles in air under M2FCT condition. We comprehensively investigated the degradation of catalysts. Our results indicate that both MEAs undergo continuous performance degradation in H 2 /air and H 2 /O 2 during the AST cycling in nitrogen, where analysis employing scanning transmission electron microscopy (STEM) and inductively coupled plasma mass spectrometry (ICP-MS) reveal significant degradation behavior for Pt catalysts. The MEA exhibits more significant degradation, especially within mass transfer region, during the AST process in air. In conclusion, this study describes the long-term degradation behavior and mechanism with AST cycling in nitrogen or air governing highly efficient and durable PGM-catalyst MEA design under HDV conditions.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Usability Evaluation Technical Report: A Usability Evaluation of the Communication Tool Prototype Prepared in Adobe XD for High Density Vertiplex (HDV) Team

Purpose: As cities get more crowded, the roadway infrastructure cannot keep up with the travel demands. Aviation can be a solution. Organizations supporting NASA’s Urban Air Mobility (UAM) concept are conducting studies on feasible concepts of operations for the new air traffic management system required to implement UAM. NASA’s High Density Vertiplex (HDV) team conducts studies using both live flights and simulations. Significance: The goal of this study is to evaluate the effectiveness of a communication tool developed for this team to enhance team situation awareness. Specifically, the study aims to determine if the communication tool is effective in allowing NASA-Langley and NASA-Ames team members playing different roles to understand what others are doing so that completed steps can be communicated to the whole team. Methods: This study asks participants to conduct four distinct scenarios using an Adobe XD prototype. Participants included five members of the target audience, who each received up to 15 minutes of training on how to use the Adobe XD prototype. The participants were NASA-Ames or NASA-Langley researchers, developers or tech leads who had 0 to 15 years of experience. Their ages ranged from 20 to 40 years old, and they were of both genders. After training, each participant was asked to do 4 tasks of varying difficulty, using the prototype testbed. After each task they reported on task difficulty and after all tasks were completed they were asked to fill out the System Usability Survey (SUS). Performance will be assessed through task completion time and accuracy rating. Results: User perception was evaluated by the SUS which showed a mean score over the six participants of 84. All the participants were above the acceptable range which was 70 and nobody was in the marginal usability zone between 50 and 70. In addition, most of the participants did well in terms of time on task, success rate and task difficulty on all the tasks except for Task 3. Participants had two minutes to do the task correctly to succeed at the task, otherwise it was marked as a failure. The geomean for Task 1 for completion time was 22.20; Task 2 was 58.28; Task 3 was 84.68 and Task 4 was 19.61. In Task 3, three out of six people failed this task but all five scored this with an average score 4.2 out of 5 for self-rating on task difficulty.

human factors↗

Examining Performance and Usability of Situation Awareness Communication Tool for NASA's High Density Vertiplex (HDV) Team

Purpose: As cities get more crowded, the roadway infrastructure cannot keep up with the travel demands. Aviation can be a solution. Organizations supporting NASA’s Urban Air Mobility (UAM) concept are conducting studies on feasible concepts of operations for the new air traffic management system required to implement UAM. NASA’s High Density Vertiplex (HDV) team conducts studies using both live flights and simulations. Significance: The goal of this study is to evaluate the effectiveness of a communication tool developed for this team to enhance team situation awareness. Specifically, the study aims to determine if the communication tool is effective in allowing NASA-Langley and NASA-Ames team members playing different roles to understand what others are doing so that completed steps can be communicated to the whole team. Methods: This study asks participants to conduct four distinct scenarios using an Adobe XD prototype. Participants included five members of the target audience, who each received up to 15 minutes of training on how to use the Adobe XD prototype. The participants were NASA-Ames or NASA-Langley researchers, developers or tech leads who had 0 to 15 years of experience. Their ages ranged from 20 to 40 years old, and they were of both genders. After training, each participant was asked to do 4 tasks of varying difficulty, using the prototype testbed. After each task they reported on task difficulty and after all tasks were completed they were asked to fill out the System Usability Survey (SUS). Performance will be assessed through task completion time and accuracy rating. Results: User perception was evaluated by the SUS which showed a mean score over the six participants of 84. All the participants were above the acceptable range which was 70 and nobody was in the marginal usability zone between 50 and 70. In addition, most of the participants did well in terms of time on task, success rate and task difficulty on all the tasks except for Task 3. Participants had two minutes to do the task correctly to succeed at the task, otherwise it was marked as a failure. The geomean for Task 1 for completion time was 22.20; Task 2 was 58.28; Task 3 was 84.68 and Task 4 was 19.61. In Task 3, three out of six people failed this task but all five scored this with an average score 4.2 out of 5 for self-rating on task difficulty.

human factors↗

Providing Levelized Cost and Waiting Time Inputs for HDV Hydrogen Refueling Station Planning: A Case Study of U.S. I-75 Corridor

Widespread use of diesel fuels in freight transportation leads to greenhouse gas (GHG) emissions that play an important role in air pollution. The air pollution, as well as the energy crisis, drives the transition from diesel fuel to cleaner and more energy-efficient fuels such as hydrogen fuels. With such a transition, the strategic planning and optimization of supporting refueling infrastructure along the national highway for heavy-duty vehicles (HDVs) become a necessity. Cost and service level are two essential factors to be considered in hydrogen refueling station location and capacity optimization. The service level in this study is assessed by the delay (waiting time) at fueling stations as well as the fueling demand fulfilled. To this end, this study presents a methodology to provide waiting time (delay) and levelized hydrogen fuel cost inputs under different station configurations (number of dispensers and fill rates) for hydrogen refueling station planning. Determining the station configurations based on the trade-off between the two inputs is further discussed. Particularly, this study points out that the levelized cost and waiting time are affected by hourly demand patterns and estimates the two inputs under different peak hour fueling demand scenarios. The results suggest that, with the same daily demand, the cost, as well as the waiting time, increases obviously with the peak hour demand. In the case study, the cost grows at least 30% when the peak hour visit frequency of the daily total visits increases from 5% (evenly distributed pattern) to 10% (the most common pattern for existing diesel fueling stations along I-75). Accounting for this peak hour effect in hydrogen refueling station planning is recommended. Overall, the waiting time model and cost analysis provide key inputs for optimizing hydrogen refueling station location and configurations based on anticipated refueling demand patterns. The paper is the second in a series that aims to build a comprehensive modeling plan and optimize hydrogen refueling infrastructure along the Interstate 75 (I-75) corridor for HDVs.

Liu, Yuandong↗

Global Interoperability of High Definition Video Streams Via ACTS and Intelsat

In 1993, a proposal at the Japan.-U.S. Cooperation in Space Program Workshop lead to a subsequent series of satellite communications experiments and demonstrations, under the title of Trans-Pacific High Data Rate Satellite Communications Experiments. The first of which is a joint collaboration between government and industry teams in the United States and Japan that successfully demonstrated distributed high definition video (HDV) post-production on a global scale using a combination of high data rate satellites and terrestrial fiber optic asynchronous transfer mode (ATM) networks. The HDV experiment is the first GIBN experiment to establish a dual-hop broadband satellite link for the transmission of digital HDV over ATM. This paper describes the team's effort in using the NASA Advanced Communications Technology Satellite (ACTS) at rates up to OC-3 (155 Mbps) between Los Angeles and Honolulu, and using Intelsat at rates up to DS-3 (45 Mbps) between Kapolei and Tokyo, with which HDV source material was transmitted between Sony Pictures High Definition Center (SPHDC) in Los Angeles and Sony Visual Communication Center (VCC) in Shinagawa, Tokyo. The global-scale connection also used terrestrial networks in Japan, the States of Hawaii and California. The 1.2 Gbps digital HDV stream was compressed down to 22.5 Mbps using a proprietary Mitsubishi MPEG-2 codec that was ATM AAL-5 compatible. The codec: employed four-way parallel processing. Improved versions of the codec are now commercially available. The successful post-production activity performed in Tokyo with a HDV clip transmitted from Los Angeles was predicated on the seamless interoperation of all the equipment between the sites, and was an exciting example in deploying a global-scale information infrastructure involving a combination of broadband satellites and terrestrial fiber optic networks. Correlation of atmospheric effects with cell loss, codec drop-out, and picture quality were made. Current efforts in the Trans-Pacific series plan to examine the use of Internet Protocol (IP)-related technologies over such an infrastructure. The use of IP allows the general public to be an integral part of the exciting activities, helps to examine issues in constructing the solar-system internet, and affords an opportunity to tap the research results from the (reliable) multicast and distributed systems communities. The current Trans- Pacific projects, including remote astronomy and digital library (visible human) are briefly described.

Hsu, Eddie↗

Comparative Evaluation of Control-Oriented Heavy Duty Vehicle Air Drag Coefficient Models

Heavy-duty vehicles (HDVs) are a significant source of fuel consumption and greenhouse gas emissions, prompting solutions such as HDV platooning to mitigate these negative impacts through air drag reduction. The intervehicle distance in an HDV platoon needs to be carefully selected, such that the platoon-level energy efficiency and safety considerations can be well balanced. Underlying this problem lies in accurately modeling the relationship between HDV air drag coefficient and intervehicle distance. Through comprehensive evaluation and comparison, we analyze five control-oriented HDV air drag coefficient models, including the polynomial model, rational polynomial model, rational model, semi-quadratic model, and ridge model. Leveraging Scipy Curve-Fit toolbox and our previously compiled air drag coefficient datasets, we optimally identify the parameters inside each model. The calibrated models are then thoroughly evaluated via five complementary metrics. The comparison results reveal that the semi-quadratic model has the highest overall performance, while the widely adopted rational model only exhibits suboptimal performance.

Best, Micah↗

Assessment of tank designs for hydrogen storage on heavy duty vehicles using metal hydrides

The objective of this project was to evaluate material-based hydrogen storage solutions as a replacement for high-pressure hydrogen gas or liquid hydrogen on Class 7 or 8 tractor fuel cell electric vehicles. The project focused on low-density main-group hydrides, a well-known class of materials for hydrogen storage. Prior research has considered metal amides as storage materials for light-duty vehicles but not for heavy-duty applications. The project established the basis for further development of storage systems of this type for heavy duty vehicles (HDV). Systems analysis of an HDV storage system comprised of a tank and associated balance of plant (piping, coolant tubes, burner) was performed to determine the usable hydrogen capacity. A composite storage material comprised of a metal hydride mixed with a high thermal-conductivity carbon is predicted to have a usable hydrogen volumetric capacity comparable to or exceeding that of 700 bar pressurized hydrogen gas. The gravimetric capacity of this material is also predicted to be competitive with pressurized gas, particularly if costly carbon fiber composite Type III or Type IV tanks are excluded. The storage system design parameters and material properties served as inputs to a second model that simulates fuel cell operation in conjunction with the storage system during an HDV drive cycle. The results show that sufficient hydrogen pressure can be produced to operate a Class 8 HDV, yielding a range of ~480 miles. These results are particularly relevant for high-impact regions, such as the South Coast Air Quality Management District, for which an economical vehicular hydrogen storage system with minimal impact on cargo capacity could accelerate adoption of heavy-duty fuel cell electric vehicles. An additional benefit is that knowledge generated by this project can assist in development of material-based storage for stationary applications such as microgrids and backup power for data centers.

08 HYDROGEN↗

Usability Evaluation of Fleet Management Interface for High Density Vertiplex Environments

To meet the rising demand for an Advanced Air Mobility (i.e. urban and rural unmanned aircraft systems) ecosystem, NASA Aeronautics Research Mission Directorate (ARMD) is hosting a series of simulations and flight tests under the High Density Vertiplex sub-project (HDV). HDV aims to develop an integrated automation architecture to support terminal area flight operations. The HDV simulations and flight tests address safety, integration, and operational challenges, while integrated systems and software demonstrate design readiness, robustness, and interoperability. During the initial HDV simulation in 2021, a prototype traffic management tool developed by NASA called Fleet Management Interface (FMI) was tested. FMI was designed to introduce an advanced level of human-automation interaction to aid both Ground Control Station Operators (GCSOs) and Fleet Managers (FMs) in remotely managing flights under their ownership. In a human-in-the-loop simulation, a usability study was conducted with FMI to identify optimal approaches for displaying information for human operators using subjective measures of usability, workload, situation awareness, risk, and trust, along with qualitative feedback. This study consisted of task analysis in which GCSO and FM subjects used an Urban Air Mobility (UAM) environment to develop and execute a plan for two different traffic scenarios of remotely controlled vehicles. In each scenario, a controlled vehicle completed a takeoff, active flight, and landing sequence while automated traffic flew in the background at a rate of 20 operations per hour. In the first scenario, the controlled vehicle flew a nominal route with takeoff and landing at the same vertiport. In the second scenario, the controlled vehicle started on the nominal route, then diverted to an unplanned location mid-flight. Results showed that self-reported performance, usability, trust, and situation awareness ratings of FMI were moderately to strongly high. There were small differences between scenarios, with Scenario 2 being perceived as more unstable, complex, variable, risky, and potentially harmful than Scenario 1. Furthermore, participants described improvements that could be made to create a better user experience. For example, users would like greater configurability of the interface based on their personal information requirements, and they would like the opportunity to review routes before assigning them. The results from this study will inform future development of the FMI with the end goal of creating a reference automation tool for airspace management procedures in AAM. The FMI could be introduced as a potential way to reduce dependency on traditional air navigation services through increased automation in high density vertiplex environments.

vertiplex↗

Lean Model-Based Systems Engineering on the NASA High-Density Vertiplex Subproject

The High Density Vertiplex (HDV) subproject of NASA’s Advanced Air Mobility (AAM) project adopted Model-Based Systems Engineering (MBSE)in July of 2020, prior to subproject formulation. A small and lean team of HDV Systems Engineers(SE) are utilizing MagicDraw to execute NASA SE processes via MBSE. The SEs learned how to use MagicDraw from scratch and HDV is the first project for which the SEs have utilized MagicDraw. This paper will demonstrate project technical execution via MBSE, utilizing the digital elements built into the SysML (Systems Modeling Language). SysML provides a model-centric means of carrying out the NASA SE common technical processes by providing tools for complete system modeling, including requirements and interface management and design capture. The authors also leverage and extend SysML to perform other SE tasks, such as Verification and Validation (V&V)tracking. MBSE has two main purposes for HDV: 1) documenting the subproject’s logical architecture for distribution outside of the subproject, 2) capturing the subproject’s physical architecture in a single-source-of-truth for use by the subproject’s members. This paper details the challenges, lessons learned, and solutions that were encountered in implementing MBSE in the first iteration on a multi-iteration, full-lifecycle design, build, fly project.

Demetrios Katsaduros↗

Usability Evaluation of Fleet Management Interface for High Density Vertiplex Environments

To meet the rising demand for an Advanced Air Mobility (AAM) (i.e. urban and rural unmanned aircraft systems) ecosystem, the NASA Aeronautics Research Mission Directorate (ARMD) is hosting a series of simulations and flight tests under the High Density Vertiplex sub-project (HDV) to prototype and study the effectiveness AAM capabilities under various operational contexts. HDV aims to develop an integrated automation architecture to support terminal area flight operations. The HDV simulations and flight tests address safety, integration, and operational challenges, while integrated systems and software demonstrate design readiness, robustness, and interoperability. During the initial HDV simulation in 2021, a prototype traffic management tool developed by NASA called the Fleet Management Interface (FMI) was tested. FMI was designed to introduce an advanced level of human-automation interaction to aid both Ground Control Station Operators (GCSOs) and Fleet Managers (FMs) in remotely managing flights under their ownership. In a human-in-the-loop simulation, a usability study was conducted with the FMI to identify optimal approaches for displaying information to human operators using subjective measures of usability, workload, situation awareness, risk, and trust, along with qualitative feedback. This study consisted of task analysis in which GCSO and FM subjects used an Urban Air Mobility (UAM) environment to develop and execute a plan for two different traffic scenarios of remotely controlled vehicles. In each scenario, a remotely controlled vehicle completed a takeoff, active flight, and landing sequence while simulated traffic flew in the background at a rate of 20 operations per hour. In the first scenario, the controlled vehicle flew a nominal route with takeoff and landing at the same vertiport. In the second scenario, the controlled vehicle started on the nominal route, then diverted to an unplanned location mid-flight. Results showed that self- reported performance, usability, trust, and situation awareness ratings of FMI were moderately to strongly high. There were small differences between scenarios, with Scenario 2 being perceived as more unstable, complex, variable, risky, and potentially harmful than Scenario 1. Furthermore, participants described improvements that could be made to create a better user experience. For example, users suggested customizable interfaces to accommodate information display preferences, and the ability to review routes before assigning them. The results from this study will inform future development of the FMI with the end goal of creating a reference automation tool for airspace management procedures in AAM. The FMI could serve to reduce dependency on traditional air navigation services through increased automation in high density vertiplex environments.

Fleet manager↗

Lean Model-Based Systems Engineering on the NASA High-Density Vertiplex Subproject

The High Density Vertiplex (HDV) subproject of NASA’s Advanced Air Mobility (AAM) project adopted Model-Based Systems Engineering (MBSE) in July of 2020, prior to subproject formulation. A small and lean team of HDV Systems Engineers (SE) are utilizing MagicDraw to execute NASA SE processes via MBSE. The SEs learned how to use MagicDraw from scratch and HDV is the first project for which the SEs have utilized MagicDraw. This presentation will demonstrate project technical execution via MBSE, utilizing the digital elements built into the SysML (Systems Modeling Language). SysML provides a model-centric means of carrying out the NASA SE common technical processes by providing tools for complete system modeling, including requirements and interface management and design capture. The authors also leverage and extend SysML to perform other SE tasks, such as Verification and Validation (V&V) tracking. MBSE has two main purposes for HDV: 1) documenting the subproject’s logical architecture for distribution outside of the subproject, 2) capturing the subproject’s physical architecture in a single-source-of-truth for use by the subproject’s members. This presentation details the challenges, lessons learned, and solutions that were encountered in implementing MBSE in the first iteration on a multi-iteration, full-lifecycle design, build, fly project.

systems engineering↗

Airspace Performance Observations of Scalable Autonomous Operations in a High Density Vertiplex Simulation

The National Aeronautics and Space Administration’s (NASA’s) High Density Vertiplex (HDV) subproject aims to develop and demonstrate progressive automation technologies that contribute to the Advanced Air Mobility (AAM) concept. Using Human-and-Hardware-In-TheLoop (HHITL) techniques, HDV demonstrates initial vertiport automation services at vertiports with increased air traffic volume in both simulated and live test environments. In 2023, the Scalable Autonomous Operations (SAO) simulation was conducted in which prototype vertiport, airspace, and ground control station technologies were assessed on technical performance. During the SAO simulation, an observational study captured an initial impression of the HDV airspace performance, potential disruptions to the airspace, and highlighted some capability and procedural gaps. Observations took place in two parts. In the first part, five scenario use cases (Nominal, Missed Approach, Speed Change, Divert, and MultiAircraft Divert) were conducted with three human operator roles (Vertiport Manager, Fleet Manager, and Ground Control Station Operator). Researchers collected metrics on throughput, closest point of approach, and airborne delay. In the second part of the study, the Missed Approach scenario was observed under three traffic density levels (20, 40, and 60 operations per hour) to challenge the automation to correctly identify slots in the vertiport arrival schedule. The results showed that the automation successfully found a slot for the Missed Approach vehicle in the 20 operations per hour condition, after some delay it found one in the 40 condition, and it did not find one in the 60 condition. The observations of technical and human performance throughout the five scenario use cases and the Missed Approach case study indicated that for HDV to increase traffic density and maintain or increase throughput, airspace monitoring services should be able to detect and resolve conflicts between aircraft. Furthermore, the roles and responsibilities of human operators need additional definition when it comes to responding to vehicle conflicts.

Advanced Air Mobility↗

Airspace Performance Observations of Scalable Autonomous Operations in a High Density Vertiplex Simulation

The National Aeronautics and Space Administration’s (NASA’s) High Density Vertiplex (HDV) sub- project aims to develop and demonstrate progressive automation technologies that contribute to the Advanced Air Mobility (AAM) concept. Using Human-and-Hardware-In-The- Loop (HHITL) techniques, HDV demonstrates initial vertiport automation services at vertiports with increased air traffic volume in both simulated and live test environments. In 2023, the Scalable Autonomous Operations (SAO) simulation was conducted in which prototype vertiport, airspace, and ground control station technologies were assessed on technical performance. During the SAO simulation, an observational study captured an initial impression of the HDV airspace performance, potential disruptions to the airspace, and highlighted some capability and procedural gaps. Observations took place in two parts. In the first part, five scenario use cases (Nominal, Missed Approach, Speed Change, Divert, and Multi- Aircraft Divert) were conducted with three human operator roles (Vertiport Manager, Fleet Manager, and Ground Control Station Operator). Researchers collected metrics on throughput, closest point of approach, and airborne delay. In the second part of the study, the Missed Approach scenario was observed under three traffic density levels (20, 40, and 60 operations per hour) to challenge the automation to correctly identify slots in the vertiport arrival schedule. The results showed that the automation successfully found a slot for the Missed Approach vehicle in the 20 operations per hour condition, after some delay it found one in the 40 condition, and it did not find one in the 60 condition. The observations of technical and human performance throughout the five scenario use cases and the Missed Approach case study indicated that for HDV to increase traffic density and maintain or increase throughput, airspace monitoring services should be able to detect and resolve conflicts between aircraft. Furthermore, the roles and responsibilities of human operators need additional definition when it comes to responding to vehicle conflicts.

advanced air mobility↗