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Designing Payload and Spaceflight Operations for Plants From Extreme 1 Terrestrial Environments

Terrestrial plants from the edges of the limits of life are likely to harbor genes that confer an advantage in deep space environments. These plants are seemingly capable of performing mission critical functions under prevailing deep space conditions while informing directed gene manipulation in target plant species. However, their adaptations to physiologically extreme habitats may hinder efficacy of routine laboratory techniques established for model plants. Here we present the development of Antarctic moss Ceratodon purpureus payload and flight operations for the ARTEMOSS experiment to the ISS astute of limited physical space and crew time. We demonstrate that the hydrophobic surface of Antarctic moss impedes chemical tissue fixation and precludes usage of RNAlater coupled with payload hardware deployed in standard plant spaceflight experiments. We show that deep-freezing the moss tissue on Petri plates provides adequate tissue fixation and allows for extraction of high-quality RNA suitable for gene expression profiling. We replaced hardware with stacks of Petri plates housing Antarctic moss and chemical fixation with deep-freeze in cryogenic GLACIER freezer. Our design can be translated to other plant species, expanding current techniques of experimentation with plants from extreme terrestrial environments aimed toward advancing human space exploration.

Agata K Zupanska

Effects of Cobalt Concentration on the Relative Resistance to Octahedral and Cube Slip in Nickel-Base Superalloys

Compression yielding tests were performed at 760 C on crystals of the Ni base superalloys Rene 150 and a modified MAR-M247, both having two different Co concentrations. For both alloy bases, increasing Co concentration was shown to decrease the critical resolved shear stress for octahedral slip, but to have little effect on that for cube slip. The results suggest that decreasing complex stacking fault energy in the gamma-prime with increasing Co could account for the observed effects.

Bobeck, Gene E.

Mechanical Design and Development of TES Bolometer Detector Arrays for the Advanced ACTPol Experiment

The next generation Advanced ACTPol (AdvACT) experiment is currently underway and will consist of four Transition Edge Sensor (TES) bolometer arrays, with three operating together, totaling 5800 detectors on the sky. Building on experience gained with the ACTPol detector arrays, AdvACT will utilize various new technologies, including 150 mm detector wafers equipped with multichroic pixels, allowing for a more densely packed focal plane. Each set of detectors includes a feedhorn array of stacked silicon wafers which form a spline pro le leading to each pixel. This is then followed by a waveguide interface plate, detector wafer, back short cavity plate, and backshort cap. Each array is housed in a custom designed structure manufactured from high purity copper and then gold plated. In addition to the detector array assembly, the array package also encloses cryogenic readout electronics. We present the full mechanical design of the AdvACT high frequency (HF) detector array package along with a detailed look at the detector array stack assemblies. This experiment will also make use of extensive hardware and software previously developed for ACT, which will be modi ed to incorporate the new AdvACT instruments. Therefore, we discuss the integration of all AdvACT arrays with pre-existing ACTPol infrastructure.

Advanced ACTPol (AdvACT)

Atacama Cosmology Telescope: Combined kinematic and thermal Sunyaev-Zel’dovich measurements from BOSS CMASS and LOWZ halos

The scattering of cosmic microwave background (CMB) photons off the free-electron gas in galaxies and clusters leaves detectable imprints on high resolution CMB maps: the thermal and kinematic Sunyaev-Zel’dovich effects (tSZ and kSZ respectively). We use combined microwave maps from the Atacama Cosmology Telescope DR5 and Planck in combination with the CMASS (mean redshifthzi¼0.55and host halo masshMviri¼3×1013M⊙) and LOWZ (hzi¼0.31,hMviri¼5×1013M⊙) galaxy catalogs from the Baryon Oscillation Spectroscopic Survey (BOSS DR10 and DR12), to study the gas associated with these galaxy groups. Using individual reconstructed velocities, we perform a stacking analysis and reject the no-kSZ hypothes is at 6.5σ, the highest significance to date. This directly translates into a measurement of the electron number density profile, and thus of the gas density profile. Despite the limited signal to noise, the measurement shows at high significance that the gas density profile is more extended than the dark matter density profile, for any reasonable baryon abundance (formally>90σfor the cosmic baryon abundance). We simultaneously measure the tSZ signal, i.e., the electron thermal pressure profile of the same CMASS objects, and reject theno-tSZ hypothesis at10σ. We combine tSZ and kSZ measurements to estimate the electron temperature to20% precision in several aperture bins, and find it comparable to the virial temperature. In a companion paper, we analyze these measurements to constrain the gas thermodynamics and the properties of feedback inside galaxy groups. We present the corresponding LOWZ measurements in this paper, ruling out a null kSZ (tSZ)signal at 2.9ð13.9Þσ, and leave their interpretation to future work. This paper and the companion paper demonstrate that current CMB experiments can detect and resolve gas profiles in low mass halos and at high redshifts, which are the most sensitive to feedback in galaxy formation and the most difficult to measure any other way. They will be a crucial input to cosmological hydrodynamical simulations, thus improving our understanding of galaxy formation. These precise gas profiles are already sufficient to reduce the main limiting theoretical systematic in galaxy-galaxy lensing: baryonic uncertainties. Future such measurements will thus unleash the statistical power of weak lensing from the Rubin, Euclid and Roman observatories. Our stacking software Thumb Stack is publicly available and directly applicable to future Simons Observatory andCMB-S4 data.

Emmanuel Schaan

Excitations for Rapidly Estimating Flight-Control Parameters

A flight test on an F-15 airplane was performed to evaluate the utility of prescribed simultaneous independent surface excitations (PreSISE) for real-time estimation of flight-control parameters, including stability and control derivatives. The ability to extract these derivatives in nearly real time is needed to support flight demonstration of intelligent flight-control system (IFCS) concepts under development at NASA, in academia, and in industry. Traditionally, flight maneuvers have been designed and executed to obtain estimates of stability and control derivatives by use of a post-flight analysis technique. For an IFCS, it is required to be able to modify control laws in real time for an aircraft that has been damaged in flight (because of combat, weather, or a system failure). The flight test included PreSISE maneuvers, during which all desired control surfaces are excited simultaneously, but at different frequencies, resulting in aircraft motions about all coordinate axes. The objectives of the test were to obtain data for post-flight analysis and to perform the analysis to determine: 1) The accuracy of derivatives estimated by use of PreSISE, 2) The required durations of PreSISE inputs, and 3) The minimum required magnitudes of PreSISE inputs. The PreSISE inputs in the flight test consisted of stacked sine-wave excitations at various frequencies, including symmetric and differential excitations of canard and stabilator control surfaces and excitations of aileron and rudder control surfaces of a highly modified F-15 airplane. Small, medium, and large excitations were tested in 15-second maneuvers at subsonic, transonic, and supersonic speeds. Typical excitations are shown in Figure 1. Flight-test data were analyzed by use of pEst, which is an industry-standard output-error technique developed by Dryden Flight Research Center. Data were also analyzed by use of Fourier-transform regression (FTR), which was developed for onboard, real-time estimation of the derivatives.

Moes, Tim

Effects of Spaceflight Relevant Carbon Dioxide Levels on Pathogenesis Related Microbial Characteristics

BACKGROUND Multiple stressors in the spaceflight environment have the potential to alter microbial pathogenesis and virulence characteristics, including microgravity, radiation, and hostile/confined conditions. This combination of factors may lead to a stacking of risks, potentially creating unexpected increase in the risk of infectious disease. One understudied variable is the higher CO2 atmospheric concentration. The current Spaceflight Maximum Allowance Concentration (SMAC) for 24-hour average CO2 is 0.4% (3 mm Hg). These elevated ambient CO2 levels aboard the ISS could potentially influence the diversity and phenotypic responses of the resident microbial communities from both the spacecraft environment (air, surface, water) and crewmembers (gut, nasal, skin microbiomes). METHODS For these studies, we will evaluate the response of Staphylococcus aureus, Streptococcus pneumoniae and Enterobacter aerogenes, which are opportunistic pathogens that are of medical significance and have been or are likely to be found aboard spacecraft. These microorganisms will be cultured in both spaceflight analog and control conditions at CO2levels representing terrestrial atmospheric concentration of 0.04% (0.3 mmHg), ISS elevated concentration of 0.4% (3 mm Hg), and an unexpected elevated concentration of 1 % (7.6 mmHg) to represent a worst case scenario. DISCUSSION Overwhelming evidence from studies performed in food microbiology, marine biology and terrestrial environmental biology suggest altered gene expression, selective bacterial inhibition, increased growth and diversity, and increased antibiotic resistance of bacterial communities individually and in biofilm formation when exposed to increased levels of CO2. While many of these findings are based on levels of CO2 higher than would be found during spaceflight exploration missions, the potential for subtle changes in CO2 levels to exacerbate infectious disease risks warrants an evaluation of pathogen responses to spaceflight CO2 conditions. Our study will provide answers to spaceflight relevant atmospheric conditions, as described in the Risk of Adverse Health Effects Due to Host-Microorganism Interaction (GapMicro 103).

A A Medina-Colorado

The Atacama Cosmology Telescope: arcminute-resolution maps of 18,000 square degrees of the microwave sky from ACT 2008-2018 data combined with Planck

This paper presents a maximum-likelihood algorithm for combining sky maps with disparate sky coverage, angular resolution and spatially varying anisotropic noise into a single map of the sky. We use this to merge hundreds of individual maps covering the 2008–2018 ACT observing seasons, resulting in by far the deepest ACT maps released so far. We also combine the maps with the full Planck maps, resulting in maps that have the best features of both Planck and ACT: Planck’s nearly white noise on intermediate and large angular scales and ACT’s high-resolution and sensitivity on small angular scales. The maps cover over 18 000 square degrees, nearly half the full sky, at 100, 150 and 220 GHz. They reveal 4 000 optically-confirmed clusters through the Sunyaev Zel’dovich effect (SZ) and 18 500 point source candidates at > 5σ, the largest single collection of SZ clusters and millimeter wave sources to date. The multi-frequency maps provide millimeter images of nearby galaxies and individual Milky Way nebulae, and even clear detections of several nearby stars. Other anticipated uses of these maps include, for example, thermal SZ and kinematic SZ cluster stacking, CMB cluster lensing and galactic dust science. The method itself has negligible bias. However, due to the preliminary nature of some of the component data sets, we caution that these maps should not be used for precision cosmological analysis. The maps are part of ACT DR5, and will be made available on LAMBDA no later than three months after the journal publication of this article, along with an interactive sky atlas.

Sigurd Naess

Superconductivity Devices: Commercial Use of Space

The work described in this report covers various aspects of the Rainbow solid-state actuator and sensor technologies. It is presented in five parts dealing with sensor applications, nonlinear properties, stress-optic and electrooptic properties, stacks and arrays, and publications. The Rainbow actuator technology is a relatively new materials development which had its inception in 1992. It involves a new processing technique for preparing pre-stressed, high lead containing piezoelectric and electrostrictive ceramic materials. Ceramics fabricated by this method produce bending-mode actuator devices which possess several times more displacement and load bearing capacity than present-day benders. Since they can also be used in sensor applications, Rainbows are part of the family of materials known as smart ceramics. During this period, PLZT Rainbow ceramics were characterized with respect to their piezoelectric properties for potential use in stress sensor applications. Studies of the nonlinear and stress-optic/electrooptic birefringent properties were also initiated during this period. Various means for increasing the utility of stress-enhanced Rainbow actuators are presently under investigation.

Haertling, Gene

The Atacama Cosmology Telescope: Weighing Distant Clusters with the Most Ancient Light

We use gravitational lensing of the cosmic microwave background (CMB) to measure the mass of the most distant blindly selected sample of galaxy clusters on which a lensing measurement has been performed to date. In CMB data from the the Atacama Cosmology Telescope and the Planck satellite, we detect the stacked lensing effect from 677 near-infrared-selected galaxy clusters from the Massive and Distant Clusters of WISE Survey (MaDCoWS), which have a mean redshift of (z)=1.08. There are currently no representative optical weak lensing measurements of clusters that match the distance and average mass of this sample. We detect the lensing signal with a significance of . We model the signal with a halo model framework to find the mean mass of the population from which these clusters are drawn. Assuming that the clusters follow Navarro–Frenk–White (NFW) density profiles, we infer a mean mass of (M(500c))=(1.7 ± 0.4) x 10^14M(ʘ). We consider systematic uncertainties from cluster redshift errors, centering errors, and the shape of the NFW profile. These are all smaller than 30% of our reported uncertainty. This work highlights the potential of CMB lensing to enable cosmological constraints from the abundance of distant clusters populating ever larger volumes of the observable universe, beyond the capabilities of optical weak lensing measurements.

Mathew S. Madhavacheril

Fractional Polarization of Extragalactic Sources in the 500 deg 2 SPTpol Survey

We study the polarization properties of extragalactic sources at 95 and 150 GHz in the SPTpol 500 deg 2 survey. We estimate the polarized power by stacking maps at known source positions, and correct for noise bias by subtracting the mean polarized power at random positions in the maps. We show that the method is unbiased using a set of simulated maps with similar noise properties to the real SPTpol maps. We find a flux-weighted mean-squared polarization fraction 〈p 2 〉 = [8.9 ± 1.1] × 10 -4 at 95 GHz and [6.9 ± 1.1] × 10 -4 at 150 GHz for the full sample. This is consistent with the values obtained for a subsample of active galactic nuclei. For dusty sources, we find 95 per cent upper limits of 〈p 2 〉95 < 16.9 × 10 -3 and 〈p 2 〉150 < 2.6 × 10 -3 . We find no evidence that the polarization fraction depends on the source flux or observing frequency. The 1σ upper limit on measured mean-squared polarization fraction at 150 GHz implies that extragalactic foregrounds will be subdominant to the CMB E and B mode polarization power spectra out to at least ℓ ≲ 5700 (ℓ ≲ 4700) and ℓ ≲ 5300 (ℓ ≲ 3600), respectively, at 95 (150) GHz.

Nikhel Gupta