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All sources NASA NTRS DOE OSTI

NASA NTRS1994

Control of cytoskeletal mechanics by extracellular matrix, cell shape, and mechanical tension

We have investigated how extracellular matrix (ECM) alters the mechanical properties of the cytoskeleton (CSK). Mechanical stresses were applied to integrin receptors on the apical surfaces of adherent endothelial cells using RGD-coated ferromagnetic microbeads (5.5-microns diameter) in conjunction with a magnetic twisting device. Increasing the number of basal cell-ECM contacts by raising the fibronectin (FN) coating density from 10 to 500 ng/cm2 promoted cell spreading by fivefold and increased CSK stiffness, apparent viscosity, and permanent deformation all by more than twofold, as measured in response to maximal stress (40 dyne/cm2). When the applied stress was increased from 7 to 40 dyne/cm2, the stiffness and apparent viscosity of the CSK increased in parallel, although cell shape, ECM contacts, nor permanent deformation was altered. Application of the same stresses over a lower number ECM contacts using smaller beads (1.4-microns diameter) resulted in decreased CSK stiffness and apparent viscosity, confirming that this technique probes into the depth of the CSK and not just the cortical membrane. When magnetic measurements were carried out using cells whose membranes were disrupted and ATP stores depleted using saponin, CSK stiffness and apparent viscosity were found to rise by approximately 20%, whereas permanent deformation decreased by more than half. Addition of ATP (250 microM) under conditions that promote CSK tension generation in membrane-permeabilized cells resulted in decreases in CSK stiffness and apparent viscosity that could be detected within 2 min after ATP addition, before any measurable change in cell size.(ABSTRACT TRUNCATED AT 250 WORDS).

Non-NASA Center↗

NASA NTRS2002

Mechanotransduction through Cytoskeleton

The goal of this project was to characterize the molecular mechanism by which cells recognize and respond to physical forces in their local environment. The project was based on the working hypothesis that cells sense mechanical stresses, such as those due to gravity, through their cell surface adhesion receptors (e.g., integrins) and that they respond as a result of structural arrangements with their internal cytoskeleton (CSK) which are orchestrated through use of tensegrity architecture. In this project, we carried out studies to define the architectural and molecular basis of cellular mechanotransduction. Our major goal was to define the molecular pathway that mediates mechanical force transfer between integrins and the CSK and to determine how mechanical deformation of integrin-CSK linkages is transduced into a biochemical response. Elucidation of the mechanism by which cells sense mechanical stresses through integrins and translate them into a biochemical response should help us to understand the molecular basis of the cellular response to gravity as well as many other forms of mechanosensation and tissue regulation. The specific aims of this proposal were: 1. To define the molecular basis of mechanical coupling between integrins, vinculin, and the actin CSK; 2. To develop a computer simulation of how mechanical stresses alter CSK structure and test this model in living cells; 3. To determine how mechanical deformation of integrin-CSK linkages is transduced into a biochemical response.

Ingber, Donald↗

NASA NTRS1986

A simple method for computing the relativistic Compton scattering kernel for radiative transfer

Correct computation of the Compton scattering kernel (CSK), defined to be the Klein-Nishina differential cross section averaged over a relativistic Maxwellian electron distribution, is reported. The CSK is analytically reduced to a single integral, which can then be rapidly evaluated using a power series expansion, asymptotic series, and rational approximation for sigma(s). The CSK calculation has application to production codes that aim at understanding certain astrophysical, laser fusion, and nuclear weapons effects phenomena.

Prasad, M. K.↗

NASA NTRS1996

Four-Dimensional Oceanic and Atmosperic Data Assimilation with Tropical Rainfall Measuring Mission Data

An oceanic data assimilation system which allows to utilize the forthcoming Tropical Rainfall Measuring Mission (TRMM) data has been developed and applied to the Pacific Ocean to produce the velocity field. The assimilated data will be indispensable to examine the effects of rainfall and its variability on the structure and circulation of the tropical oceans and to assess the impact of global warming due to the increase of carbon dioxide on the ocean circulation system and the marine pollution caused by oil spill and ocean damping of radionuclide. The data will also provide the verification for the oceanic and ocean-atmosphere coupled General Circulation Models (GCM's). The system consists of oceanic GCM, analysis scheme and data. In the system the flow field has been determined to be physically consistent with the observed density field and the sea surface winds derived from the Special Sensor Microwave Imagery (SSM/I) data which drive the ocean current. The time integration has been performed for five years until the flow field near the surface attained the steady state starting from the rest ocean with observed temperature and salinity fields, and the SSM/I surface wind velocity. The resultant flow field showed high producibility of the system. Especially the flow near the ocean surface agreed well with available observed data. The system, for the first time, succeeded to produce the eastward subtropical current which has been discovered in the joint investigation on Kuroshio current (CSK) in the 1960s. To verify the quality of the flow field a trajectory analysis has been carried out and compared with the Algos buoy data. BRIEF DESCRIPTION OF THE DATA ASSIMILATION SYSTEM ## Oceanic GCM and analysis scheme--The basic equations are much the same as used for the GCM's, except for the Newtonian damping terms introduced into the prediction equations for the potential temperature and salinity to maintain these fields as observed. The C grid of 2'lat. by 2'long. in horizontal and the 11 vertical levels are applied to the entire Pacific Ocean. At the east and west ocean boundaries the periodic boundary conditions are applied creating fictitious ocean there. The SMAC Method is used to increase the accuracy of mass conservation. * Data--The JODC temperature and salinity data obtained from 1906 to 1988 are used in the system between Long.100'E. and 60'W. The surface wind data are derived from the SSM/I data by Dr-R. Atlas of NASA/GSFC. The data set contains every 6 hours data from July 1987 to June 1989 on the grid of 2'lat. by 2.5'long. The averaged for the whole period and then interpolated into the 2'lat. by 2'long. grid data are used to force the system. The sea bottom topography data was based on the General Bathymetric Chart of the Ocean (GEBCO) supplied by the Canadian Hydrographic Service under contract with the International Hydrographic Organization and International Oceanographic Commission of UNESCO.

Takano, Kenji↗