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All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Materials Data on CSN2 by Materials Project

CN2S is alpha-like structured and crystallizes in the monoclinic P2_1/c space group. The structure is zero-dimensional and consists of four aminothiocyanate molecules. C4+ is bonded in a distorted single-bond geometry to one N1- and one S2- atom. The C–N bond length is 1.17 Å. The C–S bond length is 1.87 Å. There are two inequivalent N1- sites. In the first N1- site, N1- is bonded in a single-bond geometry to one S2- atom. The N–S bond length is 1.48 Å. In the second N1- site, N1- is bonded in a single-bond geometry to one C4+ atom. S2- is bonded in a distorted single-bond geometry to one C4+ and one N1- atom.

36 MATERIALS SCIENCE↗
DOE OSTI2020

Materials Data on CsN2 by Materials Project

NNCs crystallizes in the tetragonal P4/mbm space group. The structure is three-dimensional. Cs1+ is bonded in a 2-coordinate geometry to eight N+0.50- atoms. There are a spread of Cs–N bond distances ranging from 3.12–3.74 Å. There are three inequivalent N+0.50- sites. In the first N+0.50- site, N+0.50- is bonded in a body-centered cubic geometry to eight equivalent Cs1+ atoms. In the second N+0.50- site, N+0.50- is bonded to four equivalent Cs1+ and two equivalent N+0.50- atoms to form distorted edge-sharing NCs4N2 octahedra. Both N–N bond lengths are 1.19 Å. In the third N+0.50- site, N+0.50- is bonded in a 3-coordinate geometry to two equivalent Cs1+ and one N+0.50- atom.

36 MATERIALS SCIENCE↗
DOE OSTI2020

Materials Data on CSN2 by Materials Project

CN2S crystallizes in the monoclinic P2_1/c space group. The structure is zero-dimensional and consists of two ac1nqnjj molecules. C4+ is bonded in a distorted bent 120 degrees geometry to two N1- atoms. Both C–N bond lengths are 1.35 Å. There are two inequivalent N1- sites. In the first N1- site, N1- is bonded in a water-like geometry to one C4+ and one S2- atom. The N–S bond length is 1.63 Å. In the second N1- site, N1- is bonded in a water-like geometry to one C4+ and one S2- atom. The N–S bond length is 1.62 Å. S2- is bonded in a water-like geometry to two N1- atoms.

36 MATERIALS SCIENCE↗
DOE OSTI2021

A CRISPR-Cas9–integrase complex generates precise DNA fragments for genome integration

CRISPR-Cas9 is an RNA-guided DNA endonuclease involved in bacterial adaptive immunity and widely repurposed for genome editing in human cells, animals and plants. In bacteria, RNA molecules that guide Cas9 s activity derive from foreign DNA fragments that are captured and integrated into the host CRISPR genomic locus by the Cas1-Cas2 CRISPR integrase. How cells generate the specific lengths of DNA required for integrase capture is a central unanswered question of type II-A CRISPR-based adaptive immunity. Here, we show that an integrase supercomplex comprising guide RNA and the proteins Cas1, Cas2, Csn2 and Cas9 generates precisely trimmed 30-base pair DNA molecules required for genome integration. The HNH active site of Cas9 catalyzes exonucleolytic DNA trimming by a mechanism that is independent of the guide RNA sequence. These results show that Cas9 possesses a distinct catalytic capacity for generating immunological memory in prokaryotes.

59 BASIC BIOLOGICAL SCIENCES↗