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Results for “CHLORELLA”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS1989

Evidence for a plasma-membrane-bound nitrate reductase involved in nitrate uptake of Chlorella sorokiniana

Anti-nitrate-reductase (NR) immunoglobulin-G (IgG) fragments inhibited nitrate uptake into Chlorella cells but had no affect on nitrate uptake. Intact anti-NR serum and preimmune IgG fragments had no affect on nitrate uptake. Membrane-associated NR was detected in plasma-membrane (PM) fractions isolated by aqueous two-phase partitioning. The PM-associated NR was not removed by sonicating PM vesicles in 500 mM NaCl and 1 mM ethylenediaminetetraacetic acid and represented up to 0.8% of the total Chlorella NR activity. The PM NR was solubilized by Triton X-100 and inactivated by Chlorella NR antiserum. Plasma-membrane NR was present in ammonium-grown Chlorella cells that completely lacked soluble NR activity. The subunit sizes of the PM and soluble NRs were 60 and 95 kDa, respectively, as determined by sodium-dodecyl-sulfate electrophoresis and western blotting.

Non-NASA Center↗
NASA NTRS1961

Photosynthetic Gas Exchange in the Closed Ecosystem for Space. Phase II, Part II. Studies on the Growth of Thermophilic Chlorella 71105

Bench-scale studies with Chlorella pyrenoidosa 71105 were conducted in four- and eight-liter culture vessels, with and without recycling. It was established that the algal strain could be maintained for periods up to 72 days with supplemented re-cycled medium. In a factorial series of experiments, the highest yields were obtained with a 2.0% concentration of carbon dioxide, a 0.07 dilution factor (nutrient dilution rate/culture volume), and an 8.5 ft(exp -1) ratio of light surface to liquid culture volume. Optimum pH and concentrations of the constituents of the growth medium were determined in corollary test-tube studies. Other test-tube studies showed that the nutrient medium used for mass culture could be stored up to five weeks at 25 C or at refrigerator temperature (4 C) with little change in nitrogen-urea level. Algal suspensions stored at 4 C remained viable for periods of at least 12 weeks; growth resumed after a lag period when stored suspensions were cultured at 39 C. Streptomycin was found suitable for controlling a blue-green containment.

ALGAE↗