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At least 19 records

Nitrogen fixation and mucilage production on maize aerial roots is controlled by aerial root development and border cell functions

Exploring natural diversity for biological nitrogen fixation in maize and its progenitors is a promising approach to reducing our dependence on synthetic fertilizer and enhancing the sustainability of our cropping systems. We have shown previously that maize accessions from the Sierra Mixe can support a nitrogen-fixing community in the mucilage produced by their abundant aerial roots and obtain a significant fraction of their nitrogen from the air through these associations. In this study, we demonstrate that mucilage production depends on root cap and border cells sensing water, as observed in underground roots. The diameter of aerial roots correlates with the volume of mucilage produced and the nitrogenase activity supported by each root. Young aerial roots produce more mucilage than older ones, probably due to their root cap’s integrity and their ability to produce border cells. Transcriptome analysis on aerial roots at two different growth stages before and after mucilage production confirmed the expression of genes involved in polysaccharide synthesis and degradation. Genes related to nitrogen uptake and assimilation were up-regulated upon water exposure. Altogether, our findings suggest that in addition to the number of nodes with aerial roots reported previously, the diameter of aerial roots and abundance of border cells, polysaccharide synthesis and degradation, and nitrogen uptake are critical factors to ensure efficient nitrogen fixation in maize aerial roots.

59 BASIC BIOLOGICAL SCIENCES↗

Advancing Aerosol Chemical Characterization and Vertical Profiling over the Southern Great Plains Using Uncrewed Aerial Sampling and Offline Aerosol Mass Spectrometry

Recent advancements in uncrewed aerial systems (UASs) and particulate matter (PM) analytical techniques have provided opportunities for atmospheric research. In this study, we deployed the Department of Energy’s fixed-wing ArcticShark UAS to examine PM 2.5 composition at varying altitudes─within and above the planetary boundary layer (PBL)─over the Southern Great Plains atmospheric observatory (SGP). A total of 22 flights were conducted across March, June, and August 2023. Composite filter samples were collected during each flight and analyzed with offline aerosol mass spectrometry (AMS), complemented by on-board real-time sensors and ground-based instrumentation, to provide a comprehensive view of regional aerosol characteristics. Results show clear vertical and seasonal differences in the aerosol composition. Relative to ground-level measurements, aloft samples exhibited shifts in the distribution of organic and inorganic PM, with the organic composition varying distinctly across seasons. Particulate organic nitrogen (ON) was elevated, with bulk compositions similar in March and June but strongly altered in August, likely driven by biomass burning and enhanced photochemical activity. Combined AMS and chemical ionization mass spectrometry analyses detected amines, amides, and amino acids. PM above the planetary boundary layer was enriched in oxidized organic aerosols, while ground-level PM contained higher nitrate and sulfate. Seasonal differences in aqueous-phase processing were also observed, which were strongest in March during persistent cloud cover and weaker in the drier August period, suggesting a shift from aqueous- to gas-phase SOA formation. In conclusion, these findings highlight the value of UAS in advancing PM measurements and vertical profiling of aerosol composition.

54 ENVIRONMENTAL SCIENCES↗

The remote sensing of aquatic macrophytes Part 1: Color-infrared aerial photography as a tool for identification and mapping of littoral vegetation. Part 2: Aerial photography as a quantitative tool for the investigation of aquatic ecosystems

Research was initiated to use aerial photography as an investigative tool in studies that are part of an intensive aquatic ecosystem research effort at Lake Wingra, Madison, Wisconsin. It is anticipated that photographic techniques would supply information about the growth and distribution of littoral macrophytes with efficiency and accuracy greater than conventional methods.

Gustafson, T. D.↗

Quantifying Rates of Erosion Using Historical Aerial Photos - 19478

Erosion can play an important role in the fate and transport of waste at sites where disposal of long-lived waste is anticipated. The statistical characterization of key processes related to erosion is essential to the understanding of site stability through time. In this work multi-temporal historical aerial images are analyzed in conjunction with orthophotography and Lidar data to develop probability distributions for variables representing important erosion processes. Scans of photographic negatives were obtained for multiple photographs and several negatives were scanned with a calibrated photogrammetric scanner by Geomni (formerly Blue Skies Consulting) in Belen, New Mexico. Other negatives were provided by USDA or scanned on a high-resolution, large format media scanner in the United States National Archives Cartographic Research Room, College Park, Maryland (1939). An additional set of aerial photographs was obtained from the USGS EROS Data Center web site. For other years, photographic prints were available for aerial photography. Several prints were scanned with a large-format media scanner at 1200 dpi. Ground control points for selected digital images used in the stereoscopic analyses were identified in ArcMap. Ground control points were collected by matching features which could be identified in the aerial photography as well as the orthophotography or shaded relief imagery from the Lidar dataset. Such features included fence line intersections, road intersections, railroad bridges, and structures, depending on the age of the photography. Many old fence lines and abandoned roads remained visible in the shaded relief imagery while they were completely obscured by tree canopy in modern photography. Ground control elevations were sampled from the Lidar-based DEM or directly from point clouds. The image coordinates and matching three-dimensional projected coordinates of the ground control points were exported to IDL where parameters for a three-dimensional direct linear transformation (DLT) were obtained. Built-in singular value decomposition routines were used to solve an overdetermined set of equations for the eleven parameters of the DLT for a particular historical photograph. A solution to the DLT parameters was obtained for each historical aerial photograph in which features of interest were identified using the stereoscope. Ultimately, this process resulted in the identification of locations for features like gully heads using both the Lidar dataset and projection of the estimated location from the historical aerial photo under consideration back to the Lidar dataset. The distance between the estimate of the location from the historical image and that of the Lidar dataset was the estimated distance the feature has moved. This distance was then divided by the number of years between the Lidar dataset and the year of the historical aerial image of interest to get a rate of movement through time. This was done for several dozen points on each historical aerial image. There was inevitable uncertainty associated with the estimation of points from the historical aerial imagery. This includes estimation of changes in locations of features like gully heads through time. In this work a method to estimate the uncertainty associated with measurements of locations of features using the stereoscopic analysis is developed and applied. For features with locations in space that do not change (e.g., road intersections, etc.) the difference in measurements of locations from the Lidar dataset and that of the estimate from the historical aerial image provides an observation of the measurement error. These points used to estimate the measurement error are referred to as control points. For each of the historical aerial images, a different measurement error distribution is estimated using control points. These measurement errors are combined with the estimated distances from the stereoscopic analyses to produce distributions of gully retreat rates and hillslope erosion rates used along with output from Erosion Working Group (EWG) Landscape Evolution Models (LEMs) to provide statistical probability distributions for input into the PPA Model. Specifically, gully head retreat rate and gully widening rate were characterized using statistical probability distributions. The central limit theorem was then applied to develop rates that represent long-term averages that are used to inform a PPA Model built in GoldSim. The importance of these rates with respect to characterization of future risk can then assessed using sensitivity analyses. This provides an approach to determine if subsequent reduction of the uncertainty associated with the probability distributions of erosion rates will correspond to meaningful reduction in the estimation of future risk from the site. Ultimately this is a general method that utilizes historical aerial imagery to estimate rates of erosion with uncertainty. This information can be used to inform PPA models to project future risks from a given site. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Utilization of Local Law Enforcement Aerial Resources in Consequence Management (CM) Response

During the past decade the U.S. Department of Homeland Security (DHS) was instrumental in enhancing the nation’s ability to detect and prevent a radiological or nuclear attack in the highest risk cities. Under the DHS Securing the Cities initiative, nearly 13,000 personnel in the New York City region have been trained in preventive radiological and nuclear detection operations, and nearly 8,500 pieces of radiological detection equipment have been funded. As part of the preventive radiological/nuclear detection (PRND) mission, several cities have received funding to purchase commercial aerial radiation detection systems. In 2008, the U.S. Department of Energy, National Nuclear Security Administration Aerial Measuring System (AMS) program started providing Mobile Aerial Radiological Surveillance (MARS) training to such assets, resulting in over 150 HAZMAT teams’ officers and pilots from 10 law enforcement organizations and fire departments being trained in the aerial radiation detection. From the beginning, the MARS training course covered both the PRND and consequence management (CM) missions. Even if the law enforcement main focus is PRND, their aerial assets can be utilized in the collection of initial radiation data for post-event radiological CM response. Based on over 50 years of AMS operational experience and information collected during MARS training, this presentation will focus on the concepts of CM response using aerial assets as well as utilizing law enforcement/fire department aerial assets in CM. Also discussed will be the need for establishing closer relationships between local jurisdictions’ aerial radiation detection capabilities and state and local radiation control program directors, radiological health department managers, etc. During radiological events these individuals may become primary experts/advisers to Incident Commanders for radiological emergency response, especially in the early stages of a response. The knowledge of the existence, specific capabilities, and use of local aerial radiation detection systems would be critical in planning the response, even before federal assets arrive on the scene. The relationship between local and federal aerial assets and the potential role for the further use of the MARS training and expanded AMS Reachback capabilities in facilitating such interactions will be discussed.

consequence management, CM, local law enforcement,↗

Spectral anomaly methods for aerial detection using KUT nuisance rejection

Aerial detection combines all the challenges of standoff detection with all the difficulties of mobile survey, including large and varying standoff distances and abrupt and significant changes in background. Critical issues for aerial detection include land-water interfaces, rapidly changing KUT backgrounds, and varying down scatter with altitude changes. To address these issues a spectral anomaly method has been adapted to aerial detection. The NSCRAD (Nuisance-Rejection Spectral Comparison Ratio) algorithm was initially developed at PNNL for medium resolution detectors. The algorithm has been used for a range of ground-based detection applications, including ground-based detection, portal monitoring, and OSI applications. The method used in NSCRAD includes both optimized energy windows for detection of threat sources and a nuisance rejection capability. Additionally, the spectral anomaly method was optimized in this work for aerial detection systems to increase the probability of detection of threat sources in aerial search while using the nuisance rejection capability to minimize false alarms from rapidly changing aerial background. The adaptation of NSCRAD for aerial detection includes re-optimization of energy windows, adapting KUT nuisance rejection for aerial backgrounds, development of new methods for background tracking and adjustment of tracking and detection thresholds.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Novel Tilting Ducted-Fan Aerial Vehicle Configurations

There has been recent renewed interested in ducted-fan, powered-lift vertical takeoff and landing aircraft vehicles for uninhabited aerial vehicle and urban aerial mobility aerial applications. Early work was conducted in the 1950’s to the late 1980’s into ducted-fan vehicles, especially at the time for military missions. The focus of ducted-fan research began to shift to UAV’s and personal air transportation in the 2000’s. Work in the early 2000’s fostered interest into ducted-fan aerial vehicles for vertical lift planetary aerial vehicle missions, especially for missions to Titan, a moon of Saturn. For example, early work at NASA Ames conducted the first known investigation into oval ducts (with internal tandem fans) to be used for these aerial vehicles. The use of twin oval ducts (with internal tandem fans) allowed the use of quadrotor-style rotor rpm control for vehicle trim (in hover, for transition to high-speed forward flight the addition of a tilt-actuator to pitch the twin oval ducts forward to be aligned with the freestream needed to be added to the control approach. This current study explores open-rotor, single isolated ducted-fan, and full vehicle configuration aerodynamic performance for hover, transition, and cruise regimes for novel tilting ducted-fan aerial vehicle configurations. The objective of this study is to better understand the design aerodynamics trade space for noncircular tilting ducted-fans. The primary analysis tool used in this paper is the mid-fidelity computational fluid dynamics RotCFD tool. Several interesting and largely unexplored rotor-on-rotor (or fan-on-fan), duct-on-rotor, rotor- on-duct, and duct-on-airframe are examined in this paper. Many of these aerodynamic interactions are the key to overall aerial vehicle performance. Limited parametric sweeps of rotor-to-rotor spacing (and, therefore, sizing and shape of the enveloping ducts) for the various duct configurations will also be performed. The trade-offs between duct thrust augmentation in hover versus improved transition controllability versus improved cruise L/D will be discussed for various novel duct configurations. These ducted-fan configurations include oval ducts, ‘figure-eight’ ducts, and ‘B-shaped’ ducts, among others.

Ducted-Fan↗

Aerial Captured Data and Processed Models in Beaumont-Port Arthur Region in Feb and Oct, 2023

Our Co-design team is from the University of Texas, working on a Department of Energy-funded project focused on the Beaumont-Port Arthur area. As part of this project, we will be developing climate-resilient design solutions for areas of the region. More on www.caee.utexas.edu.We used a DJI Mavic 2 Pro to capture aerial photos in Beaumont-Port Arthur, TX, in February 2023, including:I. Beaumont Soccer ClubII. Corps’ Port Arthur Resident OfficeIII. Halbouty Pump Station comprises its vicinityIV. Lamar University (Including Exxon Power Plants close to Lamar Univ.)V. MLK Boulevard for aerial images of the industry and the ship channelVI. Salt Water Barrier (include some aerial images about the Big Thicket)Aerial photos taken were through DroneDeploy autonomous flight, and models were processed through the DroneDeploy engine as well. All aerial photos are in .JPG format and contained in zipped files for each location.The processed data package including 3D models, geospatial data, mappings, point clouds, and the animation video of Halbouty Pump Station has various file types:- The Adobe Suite gives you great software to open .Tif files.- You can use LASUtility (Windows), ESRI ArcGIS Pro (Windows), or Blaze3D (Windows, Linux) to open a LAS file and view the data it contains.- Open an .OBJ file with a large number of free and commercial applications. Some examples include Microsoft 3D Builder, Apple Preview, Blender, and Autodesk.- You may use ArcGIS, Merkaartor, Blender (with the Google Earth Importer plug-in), Global Mapper, and Marble to open .KML files.- The .tfw world file is a text file used to georeference the GeoTIFF raster images, like the orthomosaic and the DSM. You need suitable software like ArcView to open a .TFW file.This dataset provides researchers with sufficient geometric data and the status quo of the land surface at the locations mentioned above. This dataset could streamline researchers' decision-making processes and enhance the design as well.In October 2023, we had our follow-up data collection, including:I. Beaumont Soccer ClubII. Shipping and Receiving Center at Lamar UniversityAfter the aerial collection, we obtained aerial photos of those two locations mentioned above, as well as processed data (such as point clouds and models).

2D mapping↗

AERIAL MEASURING SYSTEM IN JAPAN

The U.S. Department of Energy National Nuclear Security Agency’s Aerial Measuring System deployed personnel and equipment to partner with the U.S. Air Force in Japan to conduct multiple aerial radiological surveys. These were the first and most comprehensive sources of actionable information for U.S. interests in Japan and provided early confirmation to the government of Japan as to the extent of the release from the Fukushima Daiichi Nuclear Power Generation Station. Many challenges were overcome quickly during the first 48 hours; including installation and operation of Aerial Measuring System equipment on multiple U.S. Air Force Japan aircraft, flying over difficult terrain, and flying with talented pilots who were unfamiliar with the Aerial Measuring System flight patterns. These all combined to make for a dynamic and non-textbook situation. In addition, the data challenges of the multiple and on-going releases, and integration with the Japanese government to provide valid aerial radiological survey products that both military and civilian customers could use to make informed decisions, was extremely complicated. The Aerial Measuring System Fukushima response provided insight in addressing these challenges and gave way to an opportunity for the expansion of the Aerial Measuring System’s mission beyond the borders of the US.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

A framework to enhance disaster debris estimation with AI and aerial photogrammetry

This study addresses the critical need to enhance disaster preparedness and response, focusing on hurricane impact assessment and debris estimation. Accurate assessments in this context are critical for post-event search-and-rescue (SAR) operations and resource distribution. Recent computing advancements are revealing the potential of unmanned aerial vehicles (UAVs) and artificial intelligence (AI) technologies in collecting data and assisting with post-hurricane reconnaissance. However, the use of AI and UAV photogrammetry for accurate disaster impact analysis remains underexplored. To this end, this study proposes a damage and debris analysis framework harnessing reality capture through aerial imagery and photogrammetry. Within this framework, a region-based neural network is leveraged to detect debris locations in aerial imagery with favorable performance. In a testbed within the Beaumont-Port Arthur region, in Southeast Texas, this study performs 3D reality captures of the built environment. Since the accuracy of the 3D reality capture is of importance in research areas associated with time-sensitive disaster response, we further investigate the optimal 2D aerial imagery overlap ratio required to generate a sufficiently accurate 3D model for disaster impact analysis and debris volume estimation. Results indicate that, in the case of aerial imagery for infrastructure systems, a minimum of 60 % overlap is recommended for damage assessment and debris analysis. In contrast, for flat green areas, a minimum of 50 % overlap is adequate. Overall, for disaster response applications, our study reveals that an overlap ratio between 60 % and 70 % is optimal for achieving a balance between time efficiency and data quality in aerial data collection. Furthermore, these quantitative recommendations are crucial for enabling efficient disaster response efforts. Additionally, our study outcomes will improve disaster impact analysis and facilitating timely and effective response strategies.

Artificial intelligence↗

Identification of genetic and environmental factors influencing aerial root traits that support biological nitrogen fixation in sorghum

Abstract Plant breeding and genetics play a major role in the adaptation of plants to meet human needs. The current requirement to make agriculture more sustainable can be partly met by a greater reliance on biological nitrogen fixation by symbiotic diazotrophic microorganisms that provide crop plants with ammonium. Select accessions of the cereal crop sorghum (Sorghum bicolor (L.) Moench) form mucilage-producing aerial roots that harbor nitrogen-fixing bacteria. Breeding programs aimed at developing sorghum varieties that support diazotrophs will benefit from a detailed understanding of the genetic and environmental factors contributing to aerial root formation. A genome-wide association study of the sorghum minicore, a collection of 242 landraces, and 30 accessions from the sorghum association panel was conducted in Florida and Wisconsin and under 2 fertilizer treatments to identify loci associated with the number of nodes with aerial roots and aerial root diameter. Sequence variation in genes encoding transcription factors that control phytohormone signaling and root system architecture showed significant associations with these traits. In addition, the location had a significant effect on the phenotypes. Concurrently, we developed F2 populations from crosses between bioenergy sorghums and a landrace that produced extensive aerial roots to evaluate the mode of inheritance of the loci identified by the genome-wide association study. Furthermore, the mucilage collected from aerial roots contained polysaccharides rich in galactose, arabinose, and fucose, whose composition displayed minimal variation among 10 genotypes and 2 fertilizer treatments. These combined results support the development of sorghums with the ability to acquire nitrogen via biological nitrogen fixation.

59 BASIC BIOLOGICAL SCIENCES↗