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Nitrogen derived from Combined Algal Processing supports algae cultivation for biofuels
Algae biomass, a biofuel feedstock with potential to reduce global CO 2 emissions, is currently too expensive Nitrogen, a significant input and life cycle cost, requires research on recycling and additional sources to reduce costs. Integral to nitrogen recycling is the knowledge of actual nitrogen requirements in outdoor ponds, thus data from the Unified Field Studies of the Algae Testbed Public-Private Partnership was analyzed. The data showed that Nannochloropsis oceanica used 14–24 mg/L NH 4 + per harvest cycle for robust growth. Nitrogen recycled directly from the algae conversion process will likely be the primary source of nitrogen for algae cultivation. Harvested algae can be fractionated via Combined Algal Processing (CAP), where algae are pretreated, fermented, and the fuel feedstocks (e. g. ethanol, lipids) are removed leaving a nitrogen rich slurry. Chlorella vulgaris grew robustly in nitrogen-free media supplemented with CAP residues through 4 harvest cycles with no inhibition. The amount of nitrogen as free ammonium available for recycling directly without further processing was insufficient (~4% of total needed), thus the nitrogen fixing bacteria, Azotobacter vinelandii, was investigated as a means for increasing ammonium concentration in CAP residues. This organism was shown to be proficient at growth on sugars liberated from algae biomass during pretreatment and sustained algae growth using A. vinelandii supernatants was also demonstrated. Using Azotobacter to increase ammonium concentrations on-site using an inexpensive biological process to generate the necessary ammonium for outdoor algae cultivation improves sustainability of algae biofuels by avoiding the energy intensive steps of typical ammonium production and transport. Furthermore, these results show that byproducts of algae conversion begin to close nitrogen demand and, with further supplementation by diazotrophic bacteria or protein hydrolysis, may completely replace current energyintensive and costly industrial nitrogen sources offering a path to increased economic feasibility and improved life cycle analysis of algae-based biofuels.
Energy Productivity of the High Velocity Algae Raceway Integrated Design (ARID-HV)
The ARID raceway is an effective method to maintain temperature in the optimal growing range. However, the energy input is excessive. Thus, the ARID-HV raceway was developed in order to reduce energy input requirements. This was accomplished by improving pumping efficiency and using a serpentine flow pattern in which the water flows through channels instead of over barriers. A prototype ARID-HV raceway was installed in Tucson, Arizona in order to evaluate the flow and energy requirements of the raceway. Preliminary results show continued high energy usage, but this paper explores the possible energy reductions with the new ARID-HV design with efficient propeller pumps and properly engineered channel lengths. Channel lengths are evaluated with Manning’s equation in order to determine the maximum channel length that could be implemented with the current pumping configuration
The Algae Foundation® and Algae Technology Educational Consortium
Abstract The Algae Foundation established in February 2013 has developed a diverse portfolio of algal‐based education and workforce development programs covering education levels from kindergarten through college, aquaculture extension, and free online courses. The Algae Foundation created the Algae Technology Educational Consortium (ATEC) with five major foci including community college certificate program in algae cultivation; community college curriculum adopted for algal biotechnology degree programs; Algal Massive Open Online Courses (Algal MOOCs); Algae Academy, a kindergarten to 12th grade STEM curriculum initiative; and aquaculture extension education through the Algae Cultivation Extension Short courses (ACES). The results include the education and training of over 102,000 students, aquaculturists, entrepreneurs, and bioeconomy‐based professionals aged 8–75 years in all 50 U.S. states and 66 countries. ATEC has completed agreements with 21 community colleges and universities located in Arizona, California, Connecticut, Hawaii, Louisiana, Maine, New Mexico, North Carolina, Oregon, Texas, and Washington. The first ATEC‐sponsored certificate degree program graduation was in May 2018. The Algae MOOC #1 has had over 15,752 students enrolled. ATEC initiated the Algae Academy in spring 2016 in Carlsbad, CA, and expanded to serving over 34,000 students in 46 states during the academic year 2019–2020. ACES has enrolled over 1,550 students from 66 countries.
Cis-Golgi Cisternal Assembly and Biosynthetic Activation Occur Sequentially in Plants and Algae
Abstract not provided
A new algae technical standards focus group: Summarizing and guiding the algae state of the art
The value proposition of algae technical metrics was the subject of a recent, industry-led, Focus Group interactive discussion. The interactive platform allowed for real time capturing and priority ranking of the topics that are deemed critical challenges for a future successful algae-based bioeconomy. This work ultimately sets the stage for providing guidance, methods, and standard reference materials tailored to respective algae industry segments for the cultivation, processing and marketing of high-quality and safe algae-based products. The group solicits input from the broader algae community on the value proposition and presents a case here to build a consensus around algae-specific metrics. The value proposition of technical standards to the industry is that guidance obtained through these collaborations builds producers' and consumers' trust that algae and algae-derived products are safe, reliable, and may allow for expanded market access, and increased competitiveness and international trade.
Cross-study analysis of factors affecting algae cultivation in recycled medium for biofuel production
Not provided.
Unified field studies of the algae testbed public-private partnership as the benchmark for algae agronomics
National scale agronomic projections are an important input for assessing potential benefits of algae cultivation on the future of innovative agriculture. The Algae Testbed Public-Private Partnership was established with the goal of investigating open pond algae cultivation across different geographic, climatic, seasonal, and operational conditions while setting the benchmark for quality data collection, analysis, and dissemination. Identical algae cultivation systems and data analysis methodologies were established at testbed sites across the continental United States and Hawaii. Within this framework, the Unified Field Studies were designed for algae cultivation during all 4 seasons across the testbed network. With increasingly diverse algae research and development, and field deployment strategies, the challenges associated with data collection, quality, and dissemination increase dramatically. The dataset presented here is the complete, curated, climatic, cultivation, harvest, and biomass composition data for each season at each site. Furthermore, these data enable others to do in-depth cultivation, harvest, techno-economic, life cycle, resource, and predictive growth modelling analysis, as well as development of crop protection strategies throughout the algae cultivation industry.
The algae revolution 2.0: the potential of algae for the production of food, feed, fuel and bioproducts – why we need it now
Algae made our world possible, and it can help us make the future more sustainable; but we need to change the way we live and adopt new more efficient production systems, and we need to do that now. When the world was new, the atmosphere was mainly carbon dioxide, and no animal life was possible. Along came algae with the process of photosynthesis, and things began to change. Ancient cyanobacteria algae turned carbon dioxide into enormous sums of lipids, proteins and carbohydrates, while they secreted oxygen into the atmosphere. Over a billion years, as oxygen filled the air and algae filled the seas, animal life became possible. Eventually all that algae biomass became petroleum and natural gas, which for eons sat undisturbed in vast underground reservoirs, holding enormous sums of untapped energy. Less than 200 years ago humans learned to tap these energy reserves to create the world we know today, but in so doing, we have released millions of years of stored CO 2 back into the atmosphere. Algae can again help make the world a better place, but this will require new thinking and new ways of producing our food, feed and fuels. We need an algae revolution 2.0.
Advancing the State of Technology thru DOE funded algae cultivation R&D at the Arizona Center for Algae Technology and Innovation
The Department of Energy's Bioenergy Technologies Office (BETO) has a long and successful history of funding an algae R&D portfolio driving progress towards affordable and renewable energy and co-products from algae biomass. A core capability established by BETO was the support of testbed facilities including those established under the Algae Testbed Public Private Partnership (ATP3) led by Arizona State University (ASU) and its Arizona Center for Algae Technology and Innovation (AzCATI). ATP3 established the experimental and operational framework to carry out long term cultivation trials to assess the State of Technology (SOT) for algal biomass productivity and conducted such trails thru the ending of the six year program in Spring 2018, and demonstrated steady progress increasing annual average biomass yields from 8.5 to 11.7 g/m2-day - a 38% increase from 2015 to 2018. Since the summer of 2018, the SOT framework established under ATP3 continues under another BETO funded lab-led consortium called DISCOVR "Development of Integrated Screening, Cultivar Optimization, and Verification Research." The DISCOVR team constitutes a powerful partnership among four of the DOE’s seventeen national laboratories: Los Alamos National Laboratory, National Renewable Energy Laboratory, Pacific Northwest National Laboratory (DISCOVR lead institution), and Sandia National Laboratories. With ASU’s AzCATI, they have joined forces to investigate algae strains that could lead to sustainable and clean algal biofuel and continue to generate open and transparent cultivation data sets for use by the algae biotechnology R&D community. In this talk, we will revisit the SOT results from ATP3, the SOT framework transition to DISCOVR with recent results from first full year of SOT cultivation under DISCOVR. With our recent advancements utilizing better cultivars verified thru DISCOVR’s pipeline, better crop protection and improved operational conditions, the combined effect was a further improvement in annual average productivity and reliability, leading to a year over year improvement in annual productivity from the 2018 baseline of 11.7 g/m2-day (generated across a total of 219 cultivation days) to 15.9 g/m2-day (326 cultivation days) – a 36% improvement for 2019! We will discuss these results in detail and highlight where AzCATI and the DISCOVR SOT is headed in 2020 and beyond.
Pumping Iron: A Multi-omics Analysis of Two Extremophilic Algae Reveals Iron Economy Management
Marine algae are responsible for half of the world's primary productivity, but this critical carbonsink is often constrained by insufficient iron. One species of marine algae, Dunaliella tertiolecta, isremarkable for its ability to maintain photosynthesis and thrive in low-iron environments. A relatedspecies, Dunaliella salina Bardawil, shares this attribute but is an extremophile found in hypersaline environments. To elucidate how algae manage their iron requirements, we produced highquality genome assemblies and transcriptomes for both species to serve as a foundation for acomparative multi-omics analysis. We identified a host of iron-uptake proteins in both species,including a massive expansion of transferrins and a novel family of siderophore-iron uptakeproteins. Complementing these multiple iron-uptake routes, ferredoxin functions as a large ironreservoir that can be released by induction of flavodoxin. Proteomic analysis revealed reducedinvestment in the photosynthetic apparatus coupled with remodeling of antenna proteins bydramatic iron-deficiency induction of TIDI1, a light harvesting complex protein found also in otherchlorophytes. These combinatorial iron scavenging and sparing strategies make Dunaliellaunique among photosynthetic organisms
Stochastic Forecasting of Algae Blooms in Lakes
We consider the development of harmful algae blooms (HABs) in a lake with uncertain nutrients inflow. Two general frameworks, Fokker-Planck equation and the PDF methods, are developed to quantify the resultant concentration uncertainty of various algae groups, via deriving a deterministic equation of their joint probability density function (PDF). A computational example is examined to study the evolution of cyanobacteria (the blue-green algae) and the impacts of initial concentration and inflow-outflow ratio.
Size and structure of Chlorella zofingiensis /FeCl 3 flocs in a shear flow: Algae Floc Structure
Flocculation is a promising method to overcome the economic hurdle to separation of algae from its growth medium in large scale operations. But, understanding of the floc structure and the effects of shear on the floc structure are crucial to the large scale implementation of this technique. The floc structure is important because it determines, in large part, the density and settling behavior of the algae. Freshwater algae floc size distributions and fractal dimensions are presented as a function of applied shear rate in a Couette cell using ferric chloride as a flocculant. Comparisons are made with measurements made for a polystyrene microparticle model system taken here as well as reported literature results. The algae floc size distributions are found to be self-preserving with respect to shear rate, consistent with literature data for polystyrene. Moreover, three fractal dimensions are calculated which quantitatively characterize the complexity of the floc structure. Low shear rates result in large, relatively dense packed flocs which elongate and fracture as the shear rate is increased. Our results presented here provide crucial information for economically implementing flocculation as a large scale algae harvesting strategy.
Recycled wastewater from anaerobic digestion of lipid extracted algae as a source of nutrients
Not provided.
Genetically Engineered Magnetic Algae A Leap toward Affordable Biofuel from Algae
From the earliest days of internal-combustion engines, technological visionaries dreamed about engines running on fuel made from plants. In those early days, however, it was much easier to refine fuels from crude oil, which could be obtained cheaply in many parts of the world by simply drilling a hole in the ground. However, humanity now knows that the supply of fossil fuel is limited. One day, we will run out of fossil fuel, and we will need fuel from alternative sources. One possible alternative is biofuels, but these remain a dream away today, simply because producing such fuels costs as much as $\$$10 per gallon and requires petroleum products for their production. But this long-held dream may transcend the veil of sleep into the realm of reality, thanks to the development of a technology designed to harvest algae more easily and cost effectively—algae that are major candidates for producing tomorrow’s biofuels. At Los Alamos National Laboratory, we have developed the world’s first “magnetic algae.” We say magnetic, but more specifically our genetically engineered algae are paramagnetic. Paramagnetic materials are themselves not magnetic. Instead, such materials are pulled by magnetic fields. Hence, a magnetic needle made from soft iron is actually paramagnetic, because a magnet can pull it from a haystack. For the first time ever, we have genetically modified a model algae strain to make what is called intracellular magnetic nanoparticles.
Formation of Algae Growth Constitutive Relations for Improved Algae Modeling
This SAND report summarizes research conducted as a part of a two year Laboratory Directed Research and Development (LDRD) project to improve our abilities to model algal cultivation. Algae-based biofuels have generated much excitement due to their potentially large oil yield from relatively small land use and without interfering with the food or water supply. Algae mitigate atmospheric CO 2 through metabolism. Efficient production of algal biofuels could reduce dependence on foreign oil by providing a domestic renewable energy source. Important factors controlling algal productivity include temperature, nutrient concentrations, salinity, pH, and the light-to-biomass conversion rate. Computational models allow for inexpensive predictions of algae growth kinetics in these non-ideal conditions for various bioreactor sizes and geometries without the need for multiple expensive measurement setups. However, these models need to be calibrated for each algal strain. In this work, we conduct a parametric study of key marine algae strains and apply the findings to a computational model.
Incorporation of salinity, nitrogen, and shading stress factors into the Huesemann Algae Biomass Growth model
The Huesemann Algae Biomass Growth (HABG) model predicts microalgae biomass growth in outdoor raceways as a function of water temperature and incident light intensity. The model over-predicted growth in several Regional Algal Feedstock Testbed (RAFT) experiments. Inspection of the culture conditions suggested that salinity stress, nutrient limitation, and shading reduced growth, which were not included in the original model. Laboratory experiments were carried out to measure growth response of S. obliquus to high salinity and limited nitrogen. In addition, the shading effect was estimated based on raceway geometry, solar angle, albedo and measured light intensity data. In this study, the improvement of model performance is presented step by step as each growth limiting factor is incorporated into the model. The new salinity, shanding, and nutrient stress factors remove most of the over-prediction of the original model. Comparison of the model with observed data indicated that ignoring the lag phase also resulted in overprediction. Delaying the start of the simulation until the end of the lag phase and inclusion of stress factors lowered model inaccuracy from 90% over-prediction to within ±10% in six out of seven batches.
Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes
Economical production of algal commodities (food, feed, and fuels) requires reusing cultivation water to reduce operating costs. While some algae strains show growth inhibition in reused water, other strains appear un-affected. Reusing water to grow different strains (i.e., crop rotation) could potentially improve overall biomass production compared to water reuse with the same strain by reducing accumulation of strain-specific waste products, limiting carryover of population-specific pathogens such as viruses, and introducing bacteria that degrade residual organic matter. Here, batch culture experiments tested the effects of reused cultivation water from the self-inhibiting diatom Staurosira sp. C323 on the diatom Navicula sp. SFP and green alga Chlorella sp. D046, as well as their microbiomes. Navicula sp. and Chlorella sp. grew well in Staurosira sp. reused water, indicating that previously observed self-inhibition was likely specific to this Staurosira strain. While algal mi-crobiomes were not significantly different between controls and reused water treatments, specific bacteria taxa were differentially abundant in reused treatments, suggesting that some taxa responded to compounds re-maining in the reused water. Finally, results suggest that algae growth responses in reused water may depend largely on strain-specific factors, and that crop rotation can support biomass production.