In the beginning... life assembled itself.
Evolution of life as creation of order from chaos, noting molecules self ordering capability
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Evolution of life as creation of order from chaos, noting molecules self ordering capability
Abstract Polyploidy occurs across the tree of life and is especially common in plants. Because newly formed cytotypes are often incompatible with their progenitors, polyploidy is also said to trigger “instantaneous” speciation. If a polyploid can self-fertilize or reproduce asexually, it is even possible for one individual to produce an entirely new lineage, but how often this scenario occurs is unclear. Here, we investigate the evolutionary history of the wild allotetraploid Mimulus sookensis, which was formed through hybridization between self-compatible, diploid species in the Mimulus guttatus complex. We generate a chromosome-scale reference assembly for M. sookensis and define its distinct subgenomes. Despite previous reports suggesting multiple origins of this highly selfing polyploid, we discover patterns of population genomic variation that provide unambiguous support for a single origin. One M. sookensis subgenome is clearly derived from the selfer Mimulus nasutus, which organellar variation suggests is the maternal progenitor. The ancestor of the other subgenome is less certain, but it shares variation with both Mimulus decorus and M. guttatus, two outcrossing diploids with geographic ranges that overlap broadly with M. sookensis. This study establishes M. sookensis as an example of instantaneous speciation, likely facilitated by the polyploid’s predisposition to self-fertilize.
Autonomous robotic assembly by mobile field robots has seen significant advances in recent decades, yet practicality remains elusive. Identified challenges include better use of state estimation to and reasoning with uncertainty, spreading out tasks to specialized robots, and implementing representative joining methods. This paper proposes replacing 1) self-correcting mechanical linkages with generalized joints for improved applicability, 2) assembly serial manipulators with parallel manipulators for higher precision and stability, and 3) all-in-one robots with a heterogeneous team of specialized robots for agent simplicity. This paper then describes a general assembly algorithm utilizing state estimation. Finally, these concepts are tested in the context of solar array assembly, requiring a team of robots to assemble, bond, and deploy a set of solar panel mockups to a backbone truss to an accuracy not built into the parts. This paper presents the results of these tests.
Living systems have the unique ability to form hierarchical assemblies, in which individual constituents can perform tasks cooperatively and emergently. Harnessing such properties is a long-standing challenge for the rational design of dynamic materials, that can respond to their environment, communicate with one another, and undergo a rapid, reversible, assembly through the transduction of energy. Recent developments in the design of smart and active colloidal building blocks have led to tremendous breakthroughs, with, for instance, the onset of synthetic photoactivated active assemblies. In this project, we develop a combined experimental, computational, theoretical and Machine Learning framework to shed light on the physical underpinnings of such assembly processes and program the assembly of smart active materials.
Improved cartridge-actuated impact hand tool includes a common power head and four attachments to punch holes, drive forced entry fasteners, hammer, and shear. The attachments are self-contained and easily fitted to the power head assembly.
Two flight prototype solid propellant pulsed plasma microthruster propulsion systems for the SMS satellite were fabricated, assembled and tested. The propulsion system is a completely self contained system requiring only three electrical inputs to operate: a 29.4 volt power source, a 28 volt enable signal and a 50 millsec long command fire signal that can be applied at any rate from 50 ppm to 110 ppm. The thrust level can be varied over a range 2.2 to 1 at constant impulse bit amplitude. By controlling the duration of the 28 volt enable either steady state thrust or a series of discrete impulse bits can be generated. A new technique of capacitor charging was implemented to reduce high voltage stress on energy storage capacitors.
We describe the conceptual method of an autonomously operable Direct Forming machine that would consume regolith or regolith slag to mold intimately, interlinked elements in a continuous process. The resulting product, one to three meter wide geomats, would be deployed over commonly traversed areas to isolate the astronauts and equipment from underlying dust. The porous geotextile would provide areas for dust settling, thereby mitigating dust impingement on astronaut suits or surface structures. Because of their self-supporting yet flexible structure, these geomats could be assembled into shields and buttresses to protect lunar habitants from radiation, forming a "flexoskeleton" from in situ materials.
The concept of fuel debundling proposes that all non-fuel components of a fuel assembly be removed prior to beginning the recycling process. It was introduced to reduce the quantity of and cost of disposing high level radioactive waste during recycling in the United States. However, nuclear material accountancy and control programs could also benefit from fuel debundling due to a reduction in self-shielding material for individual fuel rods compared to a fuel assembly. Current NRC regulations and guidance point to an independent fuel debundling facility with a material-possession-only license as the best option for implementing this concept into a used fuel recycling scheme in the United States. This allows the facility to comply only with simple NMAC requirements under Subpart B of 10 CFR Part 74 without the need to submit an NMAC plan to the NRC, exempting it from more tedious and complex NMAC requirements. However, a recycling facility may desire a Pu mass estimate from measurements of each individual fuel rod to improve accountancy into the dissolver. Many technologies and methodologies were considered for performing NMAC in an independent fuel debundling facility in the United States. Few were able to meet anticipated throughput demands of up to 3,300 rods/day and achieve measurement uncertainties suitable for input accountancy for a recycling facility. The best option was a combination of passive gamma-ray spectroscopy and neutron coincidence counting to meet all requirements for the proposed Fuel Debundling Detector (FUDD). Follow-on work will include a full uncertainty analysis to inform what assumptions can be made to meet input accountancy objectives before beginning experimental activities.
Propulsion systems for spacecraft, satellite communications technology, the design of large light-weight erectable structures for assembly in space, electronics and information processing for spacecraft, and self-diagnostic, fault-tolerant controls based on high memory and processing capabilities are discussed. Topics of the papers include the design of large delta wings for earth-to-orbit transports, dual-fuel propulsion units, magnetoplasmadynamic thrusters, heating rates on blunt-nosed bodies at various angles of attack, remote manipulators for space assembly tasks, solar electric propulsion for planetary missions, deployable space platforms with multiple payloads, the design of large offset-fed antennas, a nonlinear stress-strain relationship for metallic meshes, and adaptive sensors for spacecraft.
Sound engineering practice requires that fasteners and bolted joints maintain preload in service. NASA recently concluded a series of vibration tests of a multicomponent structure intended to simulate an upper stage section of a launch vehicle. The stacked components were joined through six circumferentially placed bolted cup-cone-style pyrotechnic joint mechanisms designed to share spacecraft structural loads and then enable separation during ascent. Over the course of the vibration test campaign, all six bolted cup-cone mechanisms experienced some degree of preload loss with two mechanisms losing half of their original bolt preload. A subsequent forensic anomaly investigation concluded that vibration-induced unwinding of the preload nut-and-bolt assemblies occurred despite the use of safety wire and high levels of thread friction. A series of experiments were done to better understand how large, heavily preloaded fasteners could unwind. Additionally, thread friction torque was measured and the fastener locking capability of safety wire was evaluated. The friction coefficient between the clamped cup-cone components was characterized and finally a highly instrumented mechanism-level vibration test was done to reproduce the unwinding phenomenon to better understand the mechanism's behavior. The conclusion drawn was that vibration and structural forces led to relative motion (sliding) of the clamped components, resulting in self-loosening and unwinding effects on the nut-and-bolt assembly. To counter this phenomenon, more effective fastener locking methodologies were recommended and a follow-on effort was initiated to quantify the relationship between preload, component motion, and resulting unwinding forces. It is hoped that elucidation of these effects can be used to design more effective fastener locking features.
The influence of lithium chloride (LiCl) on the hydration structure of anionic micelles of sodium dodecyl sulfate (SDS) in water was studied using the contrast-variation small-angle neutron scattering (SANS) technique. In the past, extensive computational studies have shown that the distribution of invasive water plays a critical role in the self-organization of SDS molecules and the stability of the assemblies. However, in past scattering studies the degree of the hydration level was not examined explicitly. Here, a series of contrast-variation SANS data was analyzed to extract the intramicellar radial distributions of invasive water and SDS molecules from the evolving spectral lineshapes caused by the varying isotopic ratios of water. By addressing the intramicellar inhomogeneous distributions of water and SDS molecules, a detailed description of how the counterion association influences the micellization behavior of SDS molecules is provided. The extension of our method can be used to provide an in-depth insight into the micellization phenomenon, which is commonly found in many soft matter systems.
Hydraulic-powered self propelled six degree of freedom loading vehicle moving tests assembly to test chamber with high accuracy positioning
The tooling concepts and manufacturing techniques developed to produce subelements and full-scale bonded test articles for two of the NASA Composites for Advanced Transportation Systems are examined as part of the structural development of graphite/polyimide composite material systems for the Space Shuttle Orbiter and other space transportation systems. The subassembly and the assembly bonding of the test articles were accomplished using self-tooling and low-cost aluminum tooling concepts in order to eliminate the need for complex and expensive tools. Processing procedures and tooling approaches were developed which permit controlled pressure application on specific bonding planes. Two test articles were fabricated using these techniques, an acoustic panel and a quarter segment of the Space Shuttle aft body flap.
A reusable, externally applied thermal protection system for use on aerospace vehicles subject to high thermal and mechanical stresses utilizes a shell tile structure which effectively separates its primary functions as an insulator and load absorber. The tile consists of structurally strong upper and lower metallic shells manufactured from materials meeting the thermal and structural requirements incident to tile placement on the spacecraft. A lightweight, high temperature package of insulation is utilized in the upper shell while a lightweight, low temperature insulation is utilized in the lower shell. Assembly of the tile which is facilitated by a self-locking mechanism, may occur subsequent to installation of the lower shell on the spacecraft structural skin.
A general mathematical description of the response behavior of thin-skin pneumatic envelopes and many other membrane and cable structures produces under-constrained systems that pose severe difficulties to analysis. These systems are mobile, and the general mathematical description exposes the mobility. Yet the response behavior of special under-constrained structures under special loadings can be accurately predicted using a constrained mathematical description. The static response behavior of systems that are infinitesimally mobile, such as a non-slack membrane subtended from a rigid or elastic boundary frame, can be easily analyzed using such general mathematical description as afforded by the non-linear, finite element method using an implicit solution scheme if the incremental uploading is guided through a suitable path. Similarly, if such structures are assembled with structural lack of fit that provides suitable self-stress, then dynamic response behavior can be predicted by the non-linear, finite element method and an implicit solution scheme. An explicit solution scheme is available for evolution problems. Such scheme can be used via the method of dynamic relaxation to obtain the solution to a static problem. In some sense, pneumatic envelopes and many other compliant structures can be said to have destiny under a specified loading system. What that means to the analyst is that what happens on the evolution path of the solution is irrelevant as long as equilibrium is achieved at destiny under full load and that the equilibrium is stable in the vicinity of that load. The purpose of this paper is to alert practitioners to the fact that non-standard procedures in finite element analysis are useful and can be legitimate although they burden their users with the requirement to use special caution. Some interesting findings that are useful to the US Scientific Balloon Program and that could not be obtained without non-standard techniques are presented.
We report on VERITAS very-high-energy (VHE; E >= 100 GeV) observations of six blazars selected from the Fermi Large Area Telescope First Source Catalog (1FGL). The gamma-ray emission from 1FGL sources was extrapolated up to the VHE band, taking gamma-ray absorption by the extragalactic background light into account. This allowed the selection of six bright, hard-spectrum blazars that were good candidate TeV emitters. Spectroscopic redshift measurements were attempted with the Keck Telescope for the targets without Sloan Digital Sky Survey (SDSS) spectroscopic data. No VHE emission is detected during the observations of the six sources described here. Corresponding TeV upper limits are presented, along with contemporaneous Fermi observations and non-concurrent Swift UVOT and XRT data. The blazar broadband spectral energy distributions (SEDs) are assembled and modeled with a single-zone synchrotron self-Compton model. The SED built for each of the six blazars show a synchrotron peak bordering between the intermediate- and high-spectrum-peak classifications, with four of the six resulting in particle-dominated emission region.
Metagenome binning is a key step, downstream of metagenome assembly, to group scaffolds by their genome of origin. Although accurate binning has been achieved on datasets containing multiple samples from the same community, the completeness of binning is often low in datasets with a small number of samples due to a lack of robust species co-abundance information. In this study, we exploited the chromatin conformation information obtained from Hi-C sequencing and developed a new reference-independent algorithm, Metagenome Binning with Abundance and Tetra-nucleotide frequencies—Long Range (metaBAT-LR), to improve the binning completeness of these datasets. This self-supervised algorithm builds a model from a set of high-quality genome bins to predict scaffold pairs that are likely to be derived from the same genome. Then, it applies these predictions to merge incomplete genome bins, as well as recruit unbinned scaffolds. We validated metaBAT-LR’s ability to bin-merge and recruit scaffolds on both synthetic and real-world metagenome datasets of varying complexity. Benchmarking against similar software tools suggests that metaBAT-LR uncovers unique bins that were missed by all other methods.
The blending of glassy copolymer-brush modified colloids with a viscoelastic linear copolymer featuring intrinsic self-healing enabled disordered hyperuniform hybrid materials that combined mechanical robustness with structural color, processability, environmental stability, and the ability to recover structure and properties after incurring physical damage via ‘integrated self-healing’. Symmetric linear n-butyl acrylate/methyl methacrylate (BA/MMA) were co-assembled with asymmetric glassy BA/MMA statistical copolymer brush (silica) particles. ‘Confinement-driven segregation’ resulted in a microphase-separated morphology in which the linear copolymer resided within the interstitial regions of a rigid (∼1 GPa) copolymer brush particle template with disordered hyperuniform microstructure. Diffusion of the self-healing copolymer additive into damage regions drove the recovery after damage, along with the restoration of structural color due to the materials hyperuniform microstructure. The synergistic action of intrinsic and extrinsic healing mechanisms could provide a versatile platform for the bottom-up fabrication of multifunctional hybrid materials with increased damage resistance and functional longevity.