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At least 1,765 records · Page 98

Data for Discovery, Characterization, and Application of Chromosomal Integration Sites for Stable Heterologous Gene Expression in Rhodotorula toruloides

Rhodotorula toruloides is a non-model, oleaginous yeast uniquely suited to produce acetyl-CoA-derived chemicals. However, the lack of well-characterized genomic integration sites has impeded the metabolic engineering of this organism. Here we report a set of computationally predicted and experimentally validated chromosomal integration sites in R. toruloides . We first implemented an in silico platform by integrating essential gene information and transcriptomic data to identify candidate sites that meet stringent criteria. We then conducted a full experimental characterization of these sites, assessing integration efficiency, gene expression levels, impact on cell growth, and long-term expression stability. Among the identified sites, 12 exhibited integration efficiencies of 50% or higher, making them sufficient for most metabolic engineering applications. Using selected high-efficiency sites, we achieved simultaneous double and triple integrations and efficiently integrated long functional pathways (up to 14.7 kb). Additionally, we developed a new inducible marker recycling system that allows multiple rounds of integration at our characterized sites. We validated this system by performing five sequential rounds of GFP integration and three sequential rounds of MaFAR integration for fatty alcohol production, demonstrating, for the first time, precise gene copy number tuning in R. toruloides . These characterized integration sites should significantly advance metabolic engineering efforts and future genetic tool development in R. toruloides .

Conversion↗

Transport aircraft loading and balancing system: Using a CLIPS expert system for military aircraft load planning

The requirement for improving aircraft utilization and responsiveness in airlift operations has been recognized for quite some time by the Canadian Forces. To date, the utilization of scarce airlift resources has been planned mainly through the employment of manpower-intensive manual methods in combination with the expertise of highly qualified personnel. In this paper, we address the problem of facilitating the load planning process for military aircraft cargo planes through the development of a computer-based system. We introduce TALBAS (Transport Aircraft Loading and BAlancing System), a knowledge-based system designed to assist personnel involved in preparing valid load plans for the C130 Hercules aircraft. The main features of this system which are accessible through a convivial graphical user interface, consists of the automatic generation of valid cargo arrangements given a list of items to be transported, the user-definition of load plans and the automatic validation of such load plans.

Richardson, J.↗

Developing Digital Twin Visualizations: A Methodology and Case Study on Chemical Separation Processing

As advances in digital engineering continue to push the technological boundaries, digital twin (DT) visualizations for diagnostics and safeguards advancement become much more feasible and practical. DTs generate large and complex data streams that require effective user interfaces to provide monitoring and diagnostic capabilities. Unfortunately, while these frameworks exist, there is not much research on the systematic documentation of human–computer interaction (HCI) for DT visualization. This work presents a dual-mode visualization methodology (two dimensional [2D] graphical user interface dashboard and 3D mixed reality) designed to support diagnostic tasks in DT systems and building on a validated framework and applying established HCI principles. The methodology is demonstrated through a case study of aqueous processing at Idaho National Laboratory, using experimental data from the chemical solvent extraction runs. Our interfaces display real-time alerts and monitoring to inform users of safeguards anomalies. The interfaces use immersive 3D mixed-reality visualization for further system and experiment investigation. This work demonstrates how the systematic application of HCI principles can inform DT visualization design for diagnostic and safeguards applications. While formal user evaluation studies remain as future work, this paper documents the systematic design methodology and demonstrates a proof-of-concept implementation.

3D visualization↗

Weather satellites: Stereoscopy and Sounding; Proceedings of the Topical Meeting, Ottawa, Canada, May 16-June 2, 1982

Attention is given to the validation of meteorological satellite observing systems and to stereoscopic observations from meteorological satellites. Particular papers are presented on such topics as the comparison of cloud top heights measured by airborne lidar and TIROS-N image data, the determination of surface global radiation using Meteosat images and ground-based visibility measurements, multichannel improvements to satellite-derived global sea surface temperatures, validation of the Stratospheric Sounding Unit, and the international comparison of satellite winds. Also considered is tropical storm structure revealed by stereo photographs from Skylab, stereo imaging from polar orbit and synthetic stereo imaging, simultaneous measurements of sea surface temperature by GMS-1 and GMS-2, and real-time spectroscopic applications using the GOES operational satellites.

Yates, H.↗

The Southern African Regional Science Initiative (SAFARI 2000): Overview of the Dry Season Field Campaign

The Southern African Regional Science Initiative (SAFARI 2000) is an international project investigating the earth atmosphere -human system in southern Africa. The programme was conducted over a two year period from March 1999 to March 2001. The dry season field campaign (August-September 2000) was the most intensive activity involved over 200 scientist from eighteen countries. The main objectives were to characterize and quantify biogenic, pyrogenic and anthropogenic aerosol and trace gas emissions and their transport and transformations in the atmosphere and to validate NASA's Earth Observing System's Satellite Terra within a scientific context. Five aircraft-- two South African Weather Service Aeorcommanders, the University of Washington's CV-880, the U.K. Meteorological Office's C-130, and NASA's ER-2 --with different altitude capabilities, participated in the campaign. Additional airborne sampling of southern African air masses, that had moved downwind of the subcontinent, was conducted by the CSIRO over Australia. Multiple Observations were made in various geographical sections under different synoptic conditions. Airborne missions were designed to optimize the value of synchronous over-flights of the Terra Satellite platform, above regional ground validation and science targets. Numerous smaller scale ground validation activities took place throughout the subcontinent during the campaign period.

Swap, R. J.↗

The Southern African Regional Science Initiative (SAFARI 2000). Dry-Season Campaign: An Overview

The Southern African Regional Science Initiative (SAFARI 2000) is an international science project investigating the southern African earth-atmosphere-human system. The experiment was conducted over a two-year period March 1999 - March 2001. The dry season field campaign (August-Steptember 2000) was the most intensive activity and involving over 200 scientists from 18 different nations. The main objectives of this campaign were to characterize and quantify the biogenic, pyrogenic and anthropogenic aerosol and trace gas emissions and their transport and transformations in the atmosphere and to validate the NASA Earth Observing System (EOS) satellite Terra within a scientific context. Five aircraft, namely two South African Weather Service aircraft, University of Washington CV-580, the UK Meteorological Office C-130 and the NASA ER-2, with different altitude capabilities, participated in the campaign. Additional airborne sampling of southern African air masses that had moved downwind of the subcontinent was conducted by the CSIRO over Australia. Multiple observations were taken in various sectors for a variety of synoptic conditions. Flight missions were designed to maximize synchronous over-flights of the NASA TERRA satellite platform, above regional ground validation and science targets. Numerous smaller-scale ground validation activities took place throughout the region during the campaign period.

Swap, R. J.↗

Investigation of ventilation-coupled high energy density sensible thermal energy storage

Low-cost energy storage will play an important role in supporting the decarbonization of the energy sector. Here, a novel approach to thermal energy storage for buildings is proposed, in which a tank of antifreeze liquid can be used to heat outdoor air in mechanical ventilation systems. If heated to a high enough temperature, the fluid can potentially undergo temperature swings of nearly 100°C during discharge in cold climates. Because the energy density of sensible storage systems scales proportionally to the system temperature change, this concept has the potential to offer higher energy densities than other liquid-based sensible storage devices used in building applications. A one-dimensional numerical model of the storage system was developed and experimentally validated using 30 wt% (wt%) potassium acetate as an aqueous antifreeze solution. Using a commercial hot water tank, energy densities of 47.0 kW-hour per cubic meter (kWh/m 3 ) were demonstrated in a laboratory setting. Material energy densities of approximately 74.7 (kWh/m 3 ) were measured. Design improvements may boost this energy storage density even further. Because of the system's relative simplicity, ventilation-coupled sensible storage has the potential to be an easily deployable, low-cost energy storage solution for building systems.

25 ENERGY STORAGE↗

WetLab-2: Providing Quantitative PCR Capabilities on ISS

The objective of NASA Ames Research Centers WetLab-2 Project is to place on the ISS a system capable of conducting gene expression analysis via quantitative real-time PCR (qRT-PCR) of biological specimens sampled or cultured on orbit. The WetLab-2 system is capable of processing sample types ranging from microbial cultures to animal tissues dissected on-orbit. The project has developed a RNA preparation module that can lyse cells and extract RNA of sufficient quality and quantity for use as templates in qRT-PCR reactions. Our protocol has the advantage that it uses non-toxic chemicals, alcohols or other organics. The resulting RNA is transferred into a pipette and then dispensed into reaction tubes that contain all lyophilized reagents needed to perform qRT-PCR reactions. These reaction tubes are mounted on rotors to centrifuge the liquid to the reaction window of the tube using a cordless drill. System operations require simple and limited crew actions including syringe pushes, valve turns and pipette dispenses. The resulting process takes less than 30 min to have tubes ready for loading into the qRT-PCR unit.The project has selected a Commercial-Off-The-Shelf (COTS) qRT-PCR unit, the Cepheid SmartCycler, that will fly in its COTS configuration. The SmartCycler has a number of advantages including modular design (16 independent PCR modules), low power consumption, rapid thermal ramp times and four-color detection. The ability to detect up to four fluorescent channels will enable multiplex assays that can be used to normalize for RNA concentration and integrity, and to study multiple genes of interest in each module. The WetLab-2 system will have the capability to downlink data from the ISS to the ground after a completed run and to uplink new programs. The ability to conduct qRT-PCR on-orbit eliminates the confounding effects on gene expression of reentry stresses and shock acting on live cells and organisms or the concern of RNA degradation of fixed samples. The system can be used to validate terrestrial analyses of samples returned from ISS by providing on-orbit gene expression benchmarking prior to sample return. The ability to get on-orbit data will provide investigators with the opportunity to adjust experimental parameters in real time for subsequent trials, without the need for sample return and re-flight to sample multigenerational changes. The system can also be used for analysis of air, surface, water, and clinical samples to monitor environmental contaminants and crew health. The verification flight of the instrument is scheduled to launch on SpaceX-7 in June 2015. The WetLab-2 Project is supported by NASAs ISS Program at JSC, Code OZ.

ISS tools↗

2012 Ground Testing Highlights

As part of the Fundamental Aeronautics Program and a collaborative effort with Boeing, and Lockheed Martin this past year a series of sonic boom test were completed in the NASA Ames Unitary Plan Wind Tunnel (UPWT). One of the goals was to develop new test techniques and hardware for measuring sonic boom signatures in the transonic and supersonic regimes. Data for various model designs and configurations were collected and will be used to validate CFD predictions of sonic boom signatures. Reactivation of the NASA Ames Mitsubishi compressor system was completed this past year. The compressor is intended to replace and augment the existing UPWT Clark Compressor as the primary Make Up Air (MUA) source. The MUA system provides air and vacuum pumping capability to the Ames UPWT. It will improve productivity and reliability of the UPWT as a vital testing and research facility for the U.S. aerospace industry and NASA. Funding for this task was provided from the American Recovery Investment Act (ARRA). Installation and validation of a Noncontact Stress Monitoring System (NSMS) for the 3-stage compressor was completed at the 11-foot Transonic Wind Tunnel. The system, originally developed at AEDC, consists of 36 pairs of LED light sources with optic beam send and receive probes along a 1-per rev signal. The new system allows for continuous monitoring and recording of compressor blade bending and torsion stress during normal test operations. A very unusual test was completed in the 11 FT TWT to acquire aerodynamic and flow field data for the Crew Exploration Vehicle (CEV) Parachute Assembly System (CPAS) to validate CFD methods and tools. Surface pressure distribution measurements and velocity measurements in the wake of the command module back to the drogues parachute location were acquired. Testing methods included Particle Image Velocimetry (PIV), Pressure Sensitive Paint (PSP), Schlieren Infrared Imaging (IR) and boundary layer survey and skin friction.

Buchholz, Steven J.↗

An RL10A-3-3A rocket engine model using the rocket engine transient simulator (ROCETS) software

Steady-state and transient computer models of the RL10A-3-3A rocket engine have been created using the Rocket Engine Transient Simulation (ROCETS) code. These models were created for several purposes. The RL10 engine is a critical component of past, present, and future space missions; the model will give NASA an in-house capability to simulate the performance of the engine under various operating conditions and mission profiles. The RL10 simulation activity is also an opportunity to further validate the ROCETS program. The ROCETS code is an important tool for modeling rocket engine systems at NASA Lewis. ROCETS provides a modular and general framework for simulating the steady-state and transient behavior of any desired propulsion system. Although the ROCETS code is being used in a number of different analysis and design projects within NASA, it has not been extensively validated for any system using actual test data. The RL10A-3-3A has a ten year history of test and flight applications; it should provide sufficient data to validate the ROCETS program capability. The ROCETS models of the RL10 system were created using design information provided by Pratt & Whitney, the engine manufacturer. These models are in the process of being validated using test-stand and flight data. This paper includes a brief description of the models and comparison of preliminary simulation output against flight and test-stand data.

Binder, Michael↗

To the first Shuttle launch

The major Shuttle-related testing currently in progress includes: static testing of all major elements, full scale ground vibration testing, main propulsion system testing, software verification and validation, qualification testing of many components and subsystems, and SSME verification testing. This paper reviews the current status of the Shuttle program and gives attention to the transition to operations, near-term traffic plans, user-charge policies, and payload integration. It is concluded that the transition from individual space-research projects to a fully integrated Space Transportation System, providing low-cost routine access to space for many users, involves a complete rethinking of the role NASA and the user will play.

Yardley, J. F.↗

A Spatial Analysis and Modeling System (SAMS) for environment management

This is a proposal to develop a uniform global environmental data gathering and distribution system to support the calibration and validation of remotely sensed data. SAMS is based on an enhanced version of FE MA's Integrated Emergency Management Information Systems and the Department of Defense's Air Land Battlefield Environment Software Systems. This system consists of state-of-the-art graphics and visualization techniques, simulation models, database management and expert systems for conducting environmental and disaster preparedness studies. This software package will be integrated into various Landsat and UNEP-GRID stations which are planned to become direct readout stations during the EOS timeframe. This system would be implemented as a pilot program to support the Tropical Rainfall Measuring Mission (TRMM). This will be a joint NASA-FEMA-University-Industry project.

Vermillion, Charles H.↗

Advanced Research on Integrated Energy Systems Cyber Range

As digital technologies expand to meet the needs of a more autonomous, interconnected, and advanced power system, new cybersecurity complexities and vulnerabilities arise. The ARIES Cyber Range enables the energy sector to evaluate these evolutions and validate cybersecurity solutions without impacting live systems. Combining power grid-scale hardware with emulation and simulation approaches, the ARIES Cyber Range can faithfully replicate modern energy systems - from grid physics to communication networks, and everything in between - with real-world fidelity. At NLR, researchers and partners are answering complex power system cybersecurity questions, examining emerging threats to the electric sector, and de risking new security technologies, all at a mission-relevant speed that keeps pace with rapidly evolving systems and hazards.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Medical Systems Engineering to Support Mars Mission Crew Autonomy

Human spaceflight missions to Mars face exceptionally challenging resource limitations that far exceed those faced before. Increasing transit times, decreasing opportunity for resupply, communications challenges, and extended time to evacuate a crew to definitive medical care dictate a level of crew autonomy in medical care that is beyond the current medical model. To approach this challenge, a medical systems engineering approach is proposed that relies on a clearly articulated Concept of Operations and risk analysis tools that are in development at NASA. This paper proposes an operational clinical model with key terminology and concepts translated to a controls theory paradigm to frame a common language between clinical and engineering teams. This common language will be used for design and validation of an exploration medical system that is fully integrated into a Mars transit vehicle. This approach merges medical simulation, human factors evaluation techniques, and human-in-the-loop testing in ground based analogs to tie medical hardware and software subsystem performance and overall medical system functionality to metrics of operational medical autonomy. Merging increases in operational clinical autonomy with a more restricted vehicle system resource scenario in interplanetary spaceflight will require an unprecedented level of medical and engineering integration. Full integration of medical capabilities into a Mars vehicle system may require a new approach to integrating medical system design and operations into the vehicle Program structure. Prior to the standing-up of a Mars Mission Program, proof of concept is proposed through the Human Research Program.

Antonsen, Erik↗

Sorption coolers using a continuous cycle to produce 20 K for the Planck flight mission

Two sorption coolers using hydrogen as the working fluid are currently being fabricated and assembled for flight delivery by the Jet Propulsion Laboratory (JPL). Being vibration free, scalable and with the capability for the cold end to be remotely located from the warm spacecraft are the major advantages of this class of cryo-coolers. These systems have been designed to provide a total cooling capacity (per cooler) of 1 W at a cold end temperature less than 19 K. They will be used for the Planck surveyor mission (2007 launch). In this paper we present the level Of maturity of the hydrogen sorption cooler technology at JPL by describing the design and how it has been validated at the subsystem and system levels.

sorption↗

Space Nuclear Power Autonomous Control Algorithm and Control Element Test Bed

Nuclear thermal rockets are currently NASA’s preferred option for use in a manned mission to Mars in the 2040s. The communication delay between an Earth ground station and a spacecraft heading toward Mars can be up to 20 min. Therefore, controlling the nuclear rocket engine would require either a full-time reactor operator on the mission or an autonomous control system for the reactor. The latter idea of making space nuclear reactors fully autonomous has drawn more interest from stakeholders, but such an autonomous control system must be rigorously tested and validated before it is certified for human use. The cost of a full ground test for a space nuclear reactor is tremendous, so a nonnuclear mock reactor test bed was created to test and validate control elements and control algorithms for space nuclear reactors. The test bed consists of control element hardware that inputs physical measurement data into a reactor emulator to produce the reactor’s performance under steady-state, transient, and fault conditions. The control element hardware consists of six full-sized control drums equipped with servo drives and motors and is instrumented with optical encoders, resolvers, and torque sensors for drum movement characterization. In addition to the drums, a two-phase flow loop was designed and built to mimic the valves and turbomachinery associated with the propellant flow through a nuclear thermal rocket engine; components such as pressure sensors, flow meters, thermocouples, and tachometers are instrumented throughout the loop to characterize the fluid flow, valve, and turbomachinery behavior of the system. The data from the physical hardware (e.g., drum position, propellant flow rates) are input to a nuclear reactor simulator to determine the actual nuclear reactor parameters, and the data are sent back to a control algorithm to complete the control loop. The ability to conduct numerous tests of the control systems and autonomous algorithms can help validate the instrumentation and control aspects for a space nuclear reactor for every possible fault situation.

Wilson, Brandon↗

Small Unmanned Aerial System (UAS) Flight Testing of Enabling Vehicle Technologies for the UAS Traffic Management Project

Small unmanned aerial systems (sUAS) have been studied and results indicate that there is a large array of highly-beneficial applications. These applications are too numerous to list, but include search and rescue, fire spotting, precision agriculture, etc. to name a few. Typically sUAS vehicles weigh less than 55 pounds and will be performing flight operations in the presence of manned aircraft and other sUAS. Certain sUAS applications, such as package delivery, will include operations in the close proximity of the general public. The full benefit from sUAS is contingent upon the resolution of several technological areas to enable free and widespread use of these vehicles. Technological areas in question include, but are not limited to: autonomous sense and avoid and deconfliction of sUAS from other sUAS and manned aircraft, communications and interfaces between the vehicle and human operators, and high-reliability autonomous systems. The NASA UAS Traffic Management (UTM) project is endeavoring to develop a traffic management system and concept of operations for these types of vehicles. An extensive sUAS flight test effort was performed to partially address vehicle-related technological areas and to shape an understanding of future developmental and test efforts for vehicles intended to use the UTM traffic management system. The flight testing described herein had the following objectives: 1) Install and test Dedicated Short Range Communications (DSRC) systems developed for the automotive industry for potential sense and avoid sUAS applications; 2) Evaluate the use of cellular 4G systems to provide vehicle control; 3) Obtain high-resolution video imagery in support of image-based optical detection sense and avoid systems; 4) Acquire data in fixed-wing flight to support validation and maturation of an autonomous range containment system known as Safeguard in fixed-wing flight. A total of 53 flights were performed over 12 operational days at Beaver Dam Airpark in Elberon, VA. This work was sponsored by the UTM project that is part of the Aviation Operations and Safety Program (AOSP) at NASA.

Glaab, Louis J.↗

Assurance of Model-Based Fault Diagnosis

Autonomy is an increasingly important technology for robotic scientific and commercial spacecraft. An important motivation for developing onboard autonomy is to enable quick response to dynamic environment and situations, including fault conditions that a spacecraft may encounter. The reliability of such autonomous capabilities depends on the quality of their knowledge of a spacecraft’s health state. Model-based approaches to fault management, i.e. model-based fault diagnosis (MBFD), is one approach to continuously verify correct behavior in addition to diagnosing symptoms to estimate the spacecraft’s health state. The proper functioning of MBFD is dependent on 1) the quality of the model that is analyzed and compared to the outputs of onboard sensors to estimate the system’s health state, and 2) the correct functioning of the diagnosis engine that interrogates the model and compares its analysis to observed system behavior. We are currently developing Verification and Validation (V&V) approaches to provide the necessary confidence that MBFD systems are correctly estimating the health of on-board spacecraft components and systems. Our work is intended to narrow the gap between the rapidly maturing field of ModelBased System Engineering (including MBFD) and the less-well understood area of identifying and applying appropriate V&V techniques to MBFD. Our effort is investigating three areas: 1) developing V&V techniques for the diagnostic model, 2) developing V&V techniques for the diagnostic engine in isolation, and 3) developing V&V techniques for the diagnostic engine and model in combination. This paper describes the work we have completed in the first area. We describe our approach to selecting a system to be represented by a model, the approach to modeling the system, the verification approach we developed for the model, and the results of the verification activity. We conclude with a description of the work remaining in the last two areas, which will be addressed over the next two years.

Chung, Seung↗