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At least 1,747 records · Page 97

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NASA NTRS1974

Depth of immersion as a determinant of the natriuresis of water immersion

The current study was undertaken to further assess the contribution of an immersion-induced hydrostatic pressure gradient on the redistribution of blood volume. The rate of sodium excretion by seated subjects was significantly increased by water immersion up to the chest and neck compared to waist immersion and controls. These results are consistent with the hypothesis that whereas immersion to the level of the diaphragm merely cancels the intravascular hydrostatic pressure gradient by providing an identical external gradient, immersion above the diaphragm level results in increased water pressure which tends to favor a shift in blood volume from the lower extremities.

Epstein, M.↗

NASA NTRS1990

A novel scheme to aid coherent detection of GMSK signals in fast Rayleigh fading channels

A novel scheme to insert carrier pilot to Gaussian Minimum Shift Keying (GMSK) signal using Binary Block Code (BBC) and a highpass filter in baseband is proposed. This allows the signal to be coherently demodulated even in a fast Rayleigh fading environment. As an illustrative example, the scheme is applied to a 16 kb/s GMSK signal, and its performance over a fast Rayleigh fading channel is investigated using computer simulation. This modem's 'irreducible error rate' is found to be Pe = 5.5 x 10(exp -5) which is more than that of differential detection. The modem's performance in Rician fading channel is currently under investigation.

Leung, Patrick S. K.↗

NASA NTRS1993

A satellite-borne radar wind sensor (RAWS)

Modeling global atmospheric circulations and forecasting the weather would improve if worldwide information on winds aloft were available. Accurate prediction of weather is important to agriculture, shipping, air traffic, and many other fields. Global system models of climate are of great importance. Current global atmospheric models use pressure measurements and thermodynamic properties to calculate the effects of wind for use in Numerical Weather Prediction (NWP) models. Inputs to the NWP models are temperature, pressure and wind velocities at different heights. Clearly direct wind measurements could significantly improve the NWP model performance. The RAdar Wind Sounder (RAWS) program at the University of Kansas is a study of the feasibility and the trade-offs in the design of a space-based radar system to measure wind vectors. This can be done by measuring the Doppler shift of cloud and rain returns from three or more points and calculating the components of the wind vector. The RAWS study to date uses the candidate system selected after preliminary study of frequencies and sensitivities. Two frequencies chosen, 10 and 35 GHz, allow higher sensitivity for clouds and more penetration for rain. The past year was devoted to modeling the signal-to-noise ratio (SNR) achievable for the two frequencies. The determination of SNR versus cloud penetration depth used a cloud backscattering and attenuation model in the appropriate radar equation. Calculations assumed reasonable losses in reception and transmission, in addition to the atmospheric attenuation. We discovered that ice clouds provide a higher SNR than previously calculated, but some water clouds give lower SNRs than we calculated before. One of the primary issues in the SNR calculation was the choice of the drop size distribution. Although Xin used several distributions (e.g., log normal, Khrigian and Mazin), this year we used the Deirmendjian cloud model. SNR versus cloud penetration plots were generated to validate the candidate system. Rain, which appears in the cloud models at the lower altitudes, provides ample SNR, as do the higher clouds composed of ice particles. However, in some cloud situations we found the sensitivity for the clouds was marginal or inadequate. At 35 GHz, two of the cloud models characterized by 1 to 2 g/cu m of water content at altitudes extending from 150 to 1500 meters, produced a sufficient SNR. Other models, however, with water contents ranging from 0.5 to 4 g/cu m and altitudes up to 4000 meters, exhibit SNR of -3 to -23 dB, largely because of attenuation in the upper cloud layers. These results coupled with the lower SNR at 10 GHz, led to an investigation of alternate frequencies. The rain present beneath these clouds provides adequate SNR at 10 GHz, and in most cases, at GHz.

Moore, Richard K.↗

NASA NTRS2008

Tone-Based Command of Deep Space Probes using Ground Antennas

A document discusses a technique for enabling the reception of spacecraft commands at received signal levels as much as three orders of magnitude below those of current deep space systems. Tone-based commanding deals with the reception of commands that are sent in the form of precise frequency offsets using an open-loop receiver. The key elements of this technique are an ultrastable oscillator and open-loop receiver onboard the spacecraft, both of which are part of the existing New Horizons (Pluto flyby) communications system design. This enables possible flight experimentation for tone-based commanding during the long cruise of the spacecraft to Pluto. In this technique, it is also necessary to accurately remove Doppler shift from the uplink signal presented to the spacecraft. A signal processor in the spacecraft performs a discrete Fourier transform on the received signal to determine the frequency of the received signal. Due to the long-term drift in the oscillators and orbit prediction model, the system is likely to be implemented differentially, where changes in the uplink frequency convey the command information.

Bokulic, Robert S.↗

NASA NTRS2023

Observed Changes in Hydroclimate Attributed to Human Forcing

Observational and modeling studies indicate significant changes in the global hydroclimate in the twentieth and early twenty-first centuries due to anthropogenic climate change. In this review, we analyze the recent literature on the observed changes in hydroclimate attributable to anthropogenic forcing, the physical and biological mechanisms underlying those changes, and the advantages and limitations of current detection and attribution methods. Changes in the magnitude and spatial patterns of precipitation minus evaporation ( P–E ) are consistent with increased water vapor content driven by higher temperatures. While thermodynamics explains most of the observed changes, the contribution of dynamics is not yet well constrained, especially at regional and local scales, due to limitations in observations and climate models. Anthropogenic climate change has also increased the severity and likelihood of contemporaneous droughts in southwestern North America, southwestern South America, the Mediterranean, and the Caribbean. An increased frequency of extreme precipitation events and shifts in phenology has also been attributed to anthropogenic climate change. While considerable uncertainties persist on the role of plant physiology in modulating hydroclimate and vice versa, emerging evidence indicates that increased canopy water demand and longer growing seasons negate the water-saving effects from increased water-use efficiency.

Drought↗

NASA NTRSDate not supplied

Summary of Technical Interchange Meetings (TIMs) Designed to Enable Earth Independent Medical Operations (EIMO)

The Exploration Medical Capability Element (ExMC) in NASA’s Human Research Program hosted a series of TIMs in 2023-2024 designed to stimulate discussion around specific topics with the goal of enabling EIMO. In context of the thematic constituent elements of EIMO, namely pre-mission planning, acute/emergent/prolonged medical decision making, supply/resource management and task load management, subject matter experts from industry, academia and government (NASA and other Agencies) provided valuable and actionable guidance and recommendations. Earth-based medical experts will remain indispensable for pre-mission planning, however, management of acute/emergent medical contingencies will require a gradual transition of medical care and decision making from terrestrial to space-based assets to enable support of astronaut health and performance and reduce overall mission risk. Key to achieving these enhancements is providing an integrated data system platform capable of utilizing multiple data streams in concert with a variety of on-board databases and passive monitoring of video and wearable sensors to enable a multi-modal, agentic AI-based clinical decision support system (CDSS) to support crew medical officer (CMO) medical decision-making. The EIMO series of TIMs (I-V) have proven to be instructive and portend a significant paradigm shift will be necessary to maintain crew health and performance on exploration class missions. Importantly, since the expected paradigm shift will be significantly different from the methods of operation that have been employed for the majority of missions from the inception of human spaceflight to date, any proposed methods must be deployed in the setting of ongoing operations early and be “tested, reviewed and practiced” while reliable back-up is available to facilitate an Enterprise-wide level of comfort and acceptance. Serious constraints on data transmission coupled with a large and expanding universe of on-board medical informatics data streams will necessitate implementation of a CDSS to supplant the current reliance on support provided by ground-based SMEs. Establishment of trust in the system by CMO/crew and the ground-based medical support team will be essential. Co-development of a CDSS with industry partners will assure that state of the art tools can be employed, and industry efficiencies can be leveraged. Training regimens, materials and tools must evolve to be responsive (just-in-time training) and facilitate autonomous execution of procedures. Proficiency metrics should be established and be based on validated competencies or milestones as opposed to a prescribed number of training hours. Training should be prioritized for broad, translatable skills that have universal application across a variety of medical conditions. Repetition was deemed to be the key to achieving proficiency and emphasis should lie in procedural training which is known to extinguish more rapidly than diagnostic skills. Advanced tools, e.g., extended reality, can provide more realistic and effective training. Use of advanced probabilistic risk assessment tools will be essential to optimize the medical system capability while carefully balancing risk relative to mass/power/volume limitations. Importance of factoring use-life of medical supplies and maintaining awareness of redundancy and opportunity to re-purpose under off nominal situations was emphasized. Consideration of adopting optimized performance standards vs. “good-enough” performance thresholds is warranted. The use of legacy systems as opposed to creating new systems may be preferable. Managing task load and associated cognitive load will be essential to maintain operational safety and behavioral health. ExMC aspires to create a shared EIMO paradigm and strategic vision for advancing medical system design through novel technologies, training, protocols, and support capabilities, built upon the spirit of successful strategies and innovations over the past six decades of space medicine operations.

Jay Lemery↗

DOE OSTI2019

Ionization potential depression and Pauli blocking in degenerate plasmas at extreme densities

New facilities explore warm dense matter (WDM) at conditions with extreme densities (exceeding ten times condensed matter densities) so that electrons are degenerate even at temperatures of 10 - 100 eV. Whereas in the non-degenerate region correlation effects such as Debye screening are relevant for the ionization potential depression (IPD), new effects have to be considered in degenerate plasmas. In addition to the Fock shift of the self-energies, the bound-state Pauli blocking becomes important with increasing density. Standard approaches to IPD such as Stewart-Pyatt and widely used opacity tables (e.g., OPAL) do not contain Pauli blocking effects for bound states. The consideration of degeneracy effects leads to a reduction of the ionization potential and to a higher degree of ionization. As an example, we present calculations for the ionization degree of carbon plasmas at T = 100 eV and extreme densities up to 40 g/cm 3 , which are relevant to experiments that are currently scheduled at the National Ignition Facility.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

NASA NTRSDate not supplied

Computational Tools to Design Molten Salt Electrolytes for High-Temperature Environments

Current Li-ion batteries are designed for a small operating window of 5 °C to 55 °C. Modifications to the electrolyte for operation in Mars atmosphere extended this range on the lower bound to ~ -40 °C. These operating-temperature ranges are far from those presented in high-temperature environments, such as the Venus surface, where temperatures are around 450 °C. Protecting the state-of-the-art Li-ion batteries require insulations that decreases the volumetric capacity and limiting the operational time-window. These unique challenges require a paradigm shift in materials used for designing high-temperature batteries. Molten salt electrolyte-based batteries offer a plausible route to designing high-temperature batteries. ZEBRA batteries are known to be one of the safest energy storage devices operating at 270-350 °C. Nitrate based eutectics, operating at 150 °C have also been used in Li-O2 batteries. In this study, we will examine molten-salt electrolyte transport and electrochemical properties using first-principles computations and benchmark against experiments. Further, we will present thermodynamics-based models for designing and predicting melting point of molten salt mixtures. Based on these computational tools, new molten salt mixtures designed with desired operating temperatures and electrochemical windows will be presented. Implications of these new eutectics in the context of high-temperature environment exploration will also be discussed.

Balachandran Radhakrishnan↗

NASA NTRSDate not supplied

Baseline Orbit Generation for Near Rectilinear Halo Orbits

A Near Rectilinear Halo Orbit (NRHO) with an eclipse-favorable phase serves as the current baseline orbit for the Gateway mission. The baseline provides a catalog of orbit maintenance targets and serves as a reference path for other types of trajectory analysis. In this investigation, an automated multiple-shooting differential corrections strategy is developed to generate many-revolution, long-duration, ballistic baseline orbits in a higher-fidelity ephemeris force model at the eclipse-avoidant phases and at other alternative phases. Additional geometric considerations for eclipse avoidance in the NRHO are presented and utilized within a long-lead orbit maintenance-based phase shift strategy.

Emily M. Zimovan-Spreen↗

DOE OSTI2021

Laboratory Measurements of Instrumental Signatures of the LSST Camera Focal Plane

Electro-optical testing and characterization of the Vera C. Rubin Observatory Legacy Survey of Space and Time (LSST) Camera focal plane, consisting of 205 charge-coupled devices (CCDs) arranged into 21 stand-alone Raft Tower Modules (RTMs) and 4 Corner Raft Tower Modules (CRTMs), is currently being performed at the SLAC National Accelerator Laboratory. Testing of the camera sensors is performed using a set of custom-built optical projectors, designed to illuminate the full focal plane or specific regions of the focal plane with a series of light illumination patterns: the crosstalk projector, the flat illuminator projector, and the spot grid projector. In addition to measurements of crosstalk, linearity and full well, the ability to project realistically-sized sources, using the spot grid projector, makes possible unique measurements of instrumental signatures such as deferred charge distortions, astrometric shifts due to sensor effects, and the brighter-fatter effect, prior to camera first light. Here we present the optical projector designs and usage, the electro-optical measurements and how these results have been used in testing and improving the LSST Camera instrumental signature removal algorithms.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2026

STANDARD REFERENCE MATERIAL CHARPY TESTING FROM HIGH FLUENCE SURVEILLANCE CAPSULE

The Palisades Nuclear Generating Station included in its original surveillance program a surveillance capsule, designated A-60. The capsule was removed from its surveillance position in early 1995 and has been resident in the spent fuel pool since that time. It was harvested to perform characterization of surveillance specimens in this capsule in 2023. This capsule was irradiated to a fluence of 1.96x1020 n/cm2 (E> 1MeV) that is equivalent for more than 150 effective full power years for the current US reactor pressure vessel (RPV) fleet. This capsule contained several materials, including Charpy specimens of standard reference material (SRM) from Heavy-Section Steel Technology (HSST) A533-B Plate 01. To perform Charpy testing of this highly irradiated material, the new Charpy specimen transfer system was designed and implemented on Charpy impact testing machine in the hot cell to accommodate remote testing of these specimens. This new transfer system includes integrated environmental chamber to cool or heat Charpy specimens to the desired temperature. Testing of this highly irradiated material revealed very large shift of Charpy transition temperature, 188oC.

Sokolov, Mikhail [ORNL] (ORCID:0000000256373346)↗

DOE OSTI2016

Constraining early and interacting dark energy with gravitational wave standard sirens: the potential of the eLISA mission

We perform a forecast analysis of the capability of the eLISA space-based interferometer to constrain models of early and interacting dark energy using gravitational wave standard sirens. We employ simulated catalogues of standard sirens given by merging massive black hole binaries visible by eLISA, with an electromagnetic counterpart detectable by future telescopes. We consider three-arms mission designs with arm length of 1, 2 and 5 million km, 5 years of mission duration and the best-level low frequency noise as recently tested by the LISA Pathfinder. Standard sirens with eLISA give access to an intermediate range of redshift 1 ∼< z ∼< 8, and can therefore provide competitive constraints on models where the onset of the deviation from ΛCDM (i.e. the epoch when early dark energy starts to be non-negligible, or when the interaction with dark matter begins) occurs relatively late, at z ∼< 6. If instead early or interacting dark energy is relevant already in the pre-recombination era, current cosmological probes (especially the cosmic microwave background) are more efficient than eLISA in constraining these models, except possibly in the interacting dark energy model if the energy exchange is proportional to the energy density of dark energy.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2026

Deciphering the Dynamic Balance Between Solvation Strength and Polysulfides Reaction Heterogeneity in Practical Lithium‐Sulfur Batteries

Achieving stable interfacial chemistry in lithium–sulfur batteries under practical conditions remains a key barrier to commercialization. Here, we demonstrate that interfacial dynamics can be effectively regulated by coupling solvation-power control with intrinsic heterogeneity of sulfur redox chemistry through the introduction of a weakly solvating fluorinated cosolvent, LIB 1200ET (1200ET). Compared with conventional fluorinated ethers, 1200ET efficiently shifts Li + solvation environment toward a more non-coordinated configuration at low volume fractions, enabling substantial solvation modulation without significantly impairing sulfur redox kinetics. This solvation transition weakens Li + –solvent interactions while strengthening Li + –anion and Li + –lithium polysulfide (LPS) coordination, suppressing LPS solubility and promoting reconstruction of solid–electrolyte interphase (SEI). Regulated LPS chemistry, together with 1200ET, leads to formation of a S 4+ -rich, LiF-reinforced SEI with enhanced ionic conductivity and mechanical robustness. Spatially resolved sulfur K-edge X-ray absorption spectroscopy on pouch cells reveals pronounced current-density-dependent chemical heterogeneity, distinguishing kinetically dominated and solvation-controlled regions. Under practical conditions (3.7 mg cm −2 sulfur loading, E/S = 6 µL mg −1 ), a single-layer pouch cell delivers 527 mAh g −1 over 200 cycles at C/3, while an Ah-level multilayer pouch cell achieves an energy density of 358 Wh kg −1 . These results establish non-coordinating cosolvent-driven solvation engineering as a scalable strategy for practical Li–S batteries.

36 MATERIALS SCIENCE↗

DOE OSTI2020

Blinding multiprobe cosmological experiments

ABSTRACT The goal of blinding is to hide an experiment’s critical results – here the inferred cosmological parameters – until all decisions affecting its analysis have been finalized. This is especially important in the current era of precision cosmology, when the results of any new experiment are closely scrutinized for consistency or tension with previous results. In analyses that combine multiple observational probes, like the combination of galaxy clustering and weak lensing in the Dark Energy Survey (DES), it is challenging to blind the results while retaining the ability to check for (in)consistency between different parts of the data. We propose a simple new blinding transformation, which works by modifying the summary statistics that are input to parameter estimation, such as two-point correlation functions. The transformation shifts the measured statistics to new values that are consistent with (blindly) shifted cosmological parameters while preserving internal (in)consistency. We apply the blinding transformation to simulated data for the projected DES Year 3 galaxy clustering and weak lensing analysis, demonstrating that practical blinding is achieved without significant perturbation of internal-consistency checks, as measured here by degradation of the χ2 between the data and best-fitting model. Our blinding method’s performance is expected to improve as experiments evolve to higher precision and accuracy.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

DOE OSTI2016

Brucella melitensis Methionyl-tRNA-Synthetase (MetRS), a Potential Drug Target for Brucellosis

We investigated Brucella melitensis methionyl-tRNA-synthetase (BmMetRS) with molecular, structural and phenotypic methods to learn if BmMetRS is a promising target for brucellosis drug development. Recombinant BmMetRS was expressed, purified from wild type Brucella melitensis biovar Abortus 2308 strain ATCC/CRP #DD-156 and screened by a thermal melt assay against a focused library of one hundred previously classified methionyl-tRNA-synthetase inhibitors of the blood stage form of Trypanosoma brucei. Three compounds showed appreciable shift of denaturation temperature and were selected for further studies on inhibition of the recombinant enzyme activity and cell viability against wild type B. melitensis strain 16M. BmMetRS protein complexed with these three inhibitors resolved into three-dimensional crystal structures and was analyzed. All three selected methionyl-tRNA-synthetase compounds inhibit recombinant BmMetRS enzymatic functions in an aminoacylation assay at varying concentrations. Furthermore, growth inhibition of B. melitensis strain 16M by the compounds was shown. Inhibitor-BmMetRS crystal structure models were used to illustrate the molecular basis of the enzyme inhibition. Our current data suggests that BmMetRS is a promising target for brucellosis drug development. However, further studies are needed to optimize lead compound potency, efficacy and safety as well as determine the pharmacokinetics, optimal dosage, and duration for effective treatment.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2019

Chemistry Envelope for Pitting and Stress Corrosion Cracking Mitigation

The present waste chemistry controls for the Hanford double-shell tanks (DSTs) were established in the 1980s in response to tank failures caused by stress corrosion cracking (SCC) at the DOE Savannah River Site (SRS). Portions of the SRS chemistry limits were coupled with corrosion testing results from Pacific Northwest Laboratory (PNL) and adopted for corrosion control of the DST waste at the Hanford Site. Since that time, the temperatures of many DST wastes have decreased to less than 50 °C as the radioactive material has decayed, the testing program in Argentina demonstrated that chloride ion in Hanford Site waste could cause pitting, and other work showed that dilute solutions could cause pitting at the liquid air interface (LAI). Moreover, future transfers from the single shell tanks and the waste treatment plant and process changes may shift the DST waste chemistries to a broader range of compositions with higher aggressive anion concentrations than the wastes that are presently stored. The decreasing temperatures led to the formulation of new chemistry controls for the minimization of SCC risks for operations below 50 °C in 2010, and these controls were partially adopted. The Tank Integrity Expert Panel (TIEP) Corrosion Subgroup (CSG) recommended that additional testing be performed to assess the potential for pitting and LAI corrosion. Since 2015, DNV-GL, SRNL, and the Hanford Site laboratory have investigated pitting and LAI corrosion at current and anticipated DST chemistries and Savannah River National Laboratory (SRNL) undertook a statistically based investigation of the role of nitrate and halide ion induced pitting corrosion. The objective was to develop a comprehensive waste chemistry envelope for the simultaneous minimization of the pitting and SCC risks caused by halide and nitrate ions. This report summarizes the technical basis for the new waste chemistry envelope to minimize the potential for both pitting and cracking corrosion induced by nitrate and halide ions, and the strategies used for the validation of the new controls.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2024

A Multidisciplinary Approach to Integrated Energy Systems: Advanced Nuclear Plants with Thermal Storage for Dynamic and Flexible Operation in Diverse Markets

Energy supply, distribution, and demand are continuously evolving in accordance with the integration of new power generation sources. In the United States, there is a progressive shift toward incorporating fluctuating energy sources such as wind and solar into the energy mix, alongside the distributed generation. Simultaneously, we are seeing a revolution in consumer technologies such as electric vehicles, energy devices for both residential and commercial use, and industrial processes—all contributing to a fundamental change in energy consumption dynamics. These scenarios have led to huge demand for enhanced flexibility from nuclear power plants (NPPs), requiring them to operate with unprecedented adaptability. Furthermore, advanced NPPs (A-NPPs) could potentially apply to scenarios in which power generation flexibility is prioritized over its current and conventional baseload generation capacity for traditional demand patterns. The present work covers several configurations for coupling nuclear energy production with thermal energy storage (TES). Previous work evaluated systems entailing a simple nuclear-TES configuration that used steam as a heat source for the TES of three different advanced reactors (ARs), but its heat diversion ratio (HDR) for charging was limited. The present work proposes a new configuration that features full decoupling of the nuclear reactor and the power cycle, using reactor coolant (i.e., gas) as a heat source for the TES and offering a theoretically unlimited HDR. This configuration is, however, more complex, as both high- and low-temperature TES (HT-TES and LT-TES) systems are required in order to cover the extended temperature range. Although this configuration imposes certain limitations on the discharge system configuration, it also presents the opportunity to design a more efficient system. For this case in particular, a steam reheat cycle is advantageous. Conventional TES systems take advantage of nominal efficiency when discharging, whereas the reheat cycle in the fully decoupled TES system affords a significant efficiency increase over conventional cases. Material selection, component/equipment sizing, and costing analyses were performed, followed by economic dispatch and size optimization. Dynamic models were developed for the decoupled system in order to generate insights into aspects of off design operation (e.g., drops in cycle efficiency), and these models enable exploration of potential mitigation strategies for addressing such drops. The models also highlight the need to design fail-safes that ensure continuous operational stability and minimize the impact of power ramping on the reactor parameters.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

DOE OSTI2023

Insights into the Lysine Acetylome of the Haloarchaeon Haloferax volcanii during Oxidative Stress by Quantitative SILAC-Based Proteomics

Oxidative stress adaptation strategies are important to cell function and are linked to cardiac, neurodegenerative disease, and cancer. Representatives of the Archaea domain are used as model organisms based on their extreme tolerance to oxidants and close evolutionary relationship with eukaryotes. A study of the halophilic archaeon Haloferax volcanii reveals lysine acetylation to be associated with oxidative stress responses. The strong oxidant hypochlorite: (i) stimulates an increase in lysine acetyltransferase HvPat2 to HvPat1 abundance ratios and (ii) selects for lysine deacetylase sir2 mutants. Here we report the dynamic occupancy of the lysine acetylome of glycerol-grown H. volcanii as it shifts in profile in response to hypochlorite. These findings are revealed by the: (1) quantitative multiplex proteomics of the SILAC-compatible parent and Δsir2 mutant strains and (2) label-free proteomics of H26 ‘wild type’ cells. The results show that lysine acetylation is associated with key biological processes including DNA topology, central metabolism, cobalamin biosynthesis, and translation. Lysine acetylation targets are found conserved across species. Moreover, lysine residues modified by acetylation and ubiquitin-like sampylation are identified suggesting post-translational modification (PTM) crosstalk. Overall, the results of this study expand the current knowledge of lysine acetylation in Archaea, with the long-term goal to provide a balanced evolutionary perspective of PTM systems in living organisms.

59 BASIC BIOLOGICAL SCIENCES↗