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At least 1,747 records · Page 97

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DOE OSTI2023

Transcriptional Control of hgcAB by an ArsR-Like Regulator in Pseudodesulfovibrio mercurii ND132

The hgcAB gene pair encodes mercury (Hg) methylation capability in a diverse group of microorganisms, but its evolution and transcriptional regulation remain unknown. Working from the possibility that the evolutionary function of HgcAB may not be Hg methylation, we test a possible link to arsenic resistance. Using model Hg methylator Pseudodesulfovibrio mercurii ND132, we evaluated transcriptional control of hgcAB by a putative ArsR encoded upstream and cotranscribed with hgcAB. This regulator shares homology with ArsR repressors of arsenic resistance and S-adenosylhomocysteine (SAH)- responsive regulators of methionine biosynthesis but is distinct from other ArsR/SahR proteins in P. mercurii. Using quantitative PCR (qPCR) and RNA sequencing (RNA-seq) transcriptome analyses, we confirmed this ArsR regulates hgcAB transcription and is responsive to arsenic and SAH. Additionally, RNA-seq indicated a possible link between hgcAB activity and arsenic transformations, with significant upregulation of other ArsRregulated arsenic resistance operons alongside hgcAB. Interestingly, wild-type ND132 was less sensitive to As(V) (but not As(III)) than an hgcAB knockout strain, supporting the idea that hgcAB may be linked to arsenic resistance. Arsenic significantly impacted rates of Hg methylation by ND132; however, responses varied with culture conditions. Differences in growth and metabolic activity did not account for arsenic impacts on methylation. While arsenic significantly increased hgcAB expression, hgcAB gene and transcript abundance was not a good predictor of Hg methylation rates. Taken together, these results support the idea that Hg and As cycling are linked in P. mercurii ND132. Our results may hold clues to the evolution of hgcAB and the controls on Hg methylation in nature. This work reveals a link between microbial mercury methylation and arsenic resistance and may hold clues to the evolution of mercury methylation genes (hgcAB). Microbes with hgcAB produce methylmercury, a strong neurotoxin that readily accumulates in the food web. This study addresses a critical gap in our understanding about the environmental factors that control hgcAB expression. We show that hgcAB expression is controlled by an ArsR-like regulator responsive to both arsenic and S-adenosylhomocysteine in our model organism, Pseudodesulfovibrio mercurii ND132. Exposure to arsenic also significantly impacted Pseudodesulfovibrio mercurii ND132 mercury methylation rates. However, expression of hgcAB was not always a good predictor of Hg methylation rates, highlighting the roles of Hg bioavailability and other biochemical mechanisms in methylmercury production. This study improves our understanding of the controls on hgcAB expression, which is needed to better predict environmental methylmercury production

RNA-seq↗

DOE OSTI2025

Filterbank Multicarrier Communications for High Mobility Underwater Acoustic Links

This paper presents a new method of designing prototype filters that are robust against time and frequency dispersive (i.e., doubly dispersive) underwater acoustic (UWA) channels. The proposed design uses a modified form of Slepian sequences as a basis set to arrive at a design that is maximally compact along both time and frequency/Doppler dimensions. This keeps the inter-symbol interference confined to a small number of adjacent data symbols, both across time and frequency dimensions. It also minimizes the ramp-up (at the beginning) and ramp-down (at the end) of each synthesized data packet. Here, we test our design by making use of the BELLHOP/VirTex channel simulator and compare its performance with that of the widely used orthogonal frequency division multiplexing (OFDM) method. Our emphasis is on scenarios where the channel impulse response extends to a few hundreds of milli-seconds and the relative speed of communicating vehicles can be as large as 10 m/s. We find that while OFDM fails in majority of these scenarios, the proposed design continues to work well in all the scenarios that we simulate.

97 - MATHEMATICS AND COMPUTING↗

NASA NTRS1993

Star counts from the Hubble Space Telescope Snapshot Survey. I - Galactic models

We report a photometric study of stars from 450 fields at high Galactic latitudes that were observed in the Hubble Space Telescope Snapshot Survey to an average limiting apparent magnitude of V = 21.4. There are 166 fields that contain quasars selected in radio, X-ray, and color-excess surveys. This sample of 273 stars is free of selection bias with respect to the density of stars. To within the Poisson errors, the total counts and magnitude distribution of this unbiased sample are in agreement with the Bahcall-Soneira model (Bahcall, 1986). The angular distribution of the faint stars favors, at the 2 sigma level, a somewhat steeper disk luminosity function and a smaller spheroid main-sequence normalization than given by the model. The sample does not have enough statistical power to distinguish between the two-component Bahcall-Soneira model and the three-component model first proposed by Gilmore and Reid (1983), which contains a thick disk. The statistical power of the survey would increase about 15-fold if colors were obtained for the stars: the data probe the main sequences of the disk, thick disk, and spheroid. Models with and without a thick disk could then be distinguished at the 6 sigma level. The HST Snapshot Survey includes an additional 284 fields, 279 of which are centered on quasars that were selected by objective-prism surveys. These 279 fields are expected to show and do exhibit bias against bright stars, making them unsuitable for testing Galactic models.

Gould, A.↗

NASA NTRS2001

Wind Code Application to External Forebody Flowfields with Comparisons to Experimental Results

The WIND Code, a general purpose Navier-Stokes solver, has been utilized to obtain supersonic external flowfield Computational Fluid Dynamics (CFD) solutions over an axisymmetric, parabolic forebody with comparisons made to wind tunnel experimental results. Various cases have been investigated at supersonic freestream conditions ranging from Mach 2.0 to 3.5, at 0 deg and 3 deg angles-of-attack, and with either a sharp-nose or blunt-nose forebody configuration. Both a turbulent (Baldwin-Lomax algebraic turbulence model) and a laminar model have been implemented in the CFD. Obtaining the solutions involved utilizing either the parabolized- or full-Navier-Stokes analyses supplied in WIND. Comparisons have been made with static pressure measurements, with boundary-layer rake and flowfield rake pitot pressure measurements, and with temperature sensitive paint experimental results. Using WIND's parabolized Navier-Stokes capability, grid sequencing, and the Baldwin-Lomax algebraic turbulence model allowed for significant reductions in computational time while still providing good agreement with experiment. Given that CFD and experiment compare well, WIND is found to be a good computational platform for solving this type of forebody problem, and the grids developed in conjunction with it will be used in the future to investigate varying freestream conditions not tested experimentally.

Frate, F. C.↗

NASA NTRS2003

RSRM Case Acceptance and Refurbishment Requirements

The RSRM (Reuseable Solid Rocket Motor) Program has been developed and implemented to support manned flight space travel. The Space Shuttle Vehicle utilizes two SRBs (Solid Rocket Boosters) to assist during the launch sequence and achieve the predefined orbit and mission objectives. A critical feature of this program involves post- flight SRB retrieval, disassembly and reuse of case structural components to manufacture future RSRM motors. To ensure these components can be safely reused, inspection criteria based on dimensional, structural and fracture requirements, has been established in the form of Engineering specifications and drawings. These criteria originated from actual hardware testing (both subscale and full scale) coupled with conventional Engineering hand calculations and computerized Finite Element Analyses (FEA). The intent of this paper is to provide an overview of the RSRM Case component refurbishment requirements and the associated case hardware inspection and evaluation processes that has been established to satisfy these requirements. Many of these processes have been recently upgraded to comply with environmental regulations, obsolescence concerns and technological advancements. Qualification of these process changes has been closely monitored and documented through test plans and reports.

Brinkerhoff, Gary Z.↗

NASA NTRS2006

A Numerical and Experimental Study of Compression-Loaded Composite Panels With Cutouts

Results from a numerical and experimental study on the effects of laminate orthotropy and circular cutout size on the response of compression-loaded composite curved panels are presented. Several 60-in-radius composite panels with four different laminate configurations were tested with cutout diameters that range from 10% to 60% of the panel width. Finite-element analyses were performed for each panel in order to identify the effects boundary conditions, measured initial geometric imperfections and thickness variations had on the nonlinear and buckling behavior of the panels. The compression-loaded panels considered herein exhibited two separate types of behavior depending on the laminate stacking sequence and cutout size. More specifically, some of the panels exhibited the classical snap-through type buckling response; however, some of the panels exhibited a monotonically increasing stable response and achieved compressive loads in excess of twice the predicted linear bifurcation buckling load. In general, the finite-element analyses were able to predict accurately the nonlinear response and buckling loads of the panels and the prebuckling and postbuckling out-of-plane deformations and strains.

Thornburgh, Robert P.↗

NASA NTRS2017

Image Navigation and Registration Performance Assessment Evaluation Tools for GOES-R ABI and GLM

The GOES-R Flight Project has developed an Image Navigation and Registration (INR) Performance Assessment Tool Set (IPATS) for measuring Advanced Baseline Imager (ABI) and Geostationary Lightning Mapper (GLM) INR performance metrics in the post-launch period for performance evaluation and long term monitoring. IPATS utilizes a modular algorithmic design to allow user selection of data processing sequences optimized for generation of each INR metric. This novel modular approach minimizes duplication of common processing elements, thereby maximizing code efficiency and speed. Fast processing is essential given the large number of sub-image registrations required to generate INR metrics for the many images produced over a 24 hour evaluation period. This paper describes the software design and implementation of IPATS and provides preliminary test results.

Image registration↗

NASA NTRS2017

Image Navigation and Registration Performance Assessment Evaluation Tools for GOES-R ABI and GLM

image registration↗

NASA NTRS1970

The STAR computer - A self-testing-and- repairing computer for spacecraft guidance, control, and automatic maintenance

Successful planetary exploration missions have been completed by unmanned Mariner spacecraft, which were designed at the Jet Propulsion Laboratory. Mariner II completed a 109-day flyby mission to Venus on December 14, 1962. This mission was followed by the flyby of Mars and return of 21 historic photographs of its surface by Mariner IV in July 1965. Further successful missions followed: Mariner V to Venus in 1967, Mariners VI and VII to Mars in 1969. In all of these missions, a Central Computer and Sequencer onboard the spacecraft was used to provide on-board control functions, although complete ground backup was also available.

Algirdas Avizienist↗

NASA NTRS2012

Calibration and Sequence Development Status for the Sample Analysis at Mars Investigation on the Mars Science Laboratory

The measurement goals of the Sample Analysis at Mars (SAM) instrument suite on the "Curiosity" Rover of the Mars Science Laboratory (MSL) include chemical and isotopic analysis of organic and inorganic volatiles for both atmospheric and solid samples [1,2]. SAM directly supports the ambitious goals of the MSL mission to provide a quantitative assessment of habitability and preservation in Gale crater by means of a range of chemical and geological measurements [3]. The SAM FM combined calibration and environmental testing took place primarily in 2010 with a limited set of tests implemented after integration into the rover in January 2011. The scope of SAM FM testing was limited both to preserve SAM consumables such as life time of its electromechanical elements and to minimize the level of terrestrial contamination in the SAM instrument. A more comprehensive calibration of a SAM-like suite of instruments will be implemented in 2012 with calibration runs planned for the SAM testbed. The SAM Testbed is nearly identical to the SAM FM and operates in a ambient pressure chamber. The SAM Instrument Suite: SAM's instruments are a Quadrupole Mass Spectrometer (QMS), a 6-column Gas Chromatograph (GC), and a 2-channel Tunable Laser Spectrometer (TLS). Gas Chromatography Mass Spectrometry is designed for identification of even trace organic compounds. The TLS [5] secures the C, H, and O isotopic composition in carbon dioxide, water, and methane. Sieved materials are delivered from the MSL sample acquisition and processing system to one of68 cups of the Sample Manipulation System (SMS). 59 of these cups are fabricated from inert quartz. After sample delivery, a cup is inserted into one of 2 ovens for evolved gas analysis (EGA ambient to >9500C) by the QMS and TLS. A portion of the gas released can be trapped and subsequently analyzed by GCMS. Nine sealed cups contain liquid solvents and chemical derivatization or thermochemolysis agents to extract and transform polar molecules such as amino acids, nucleobases, and carboxylic acids into compounds that are sufficiently volatile to transmit through the GC columns. The remaining 6 cups contain calibrants. SAM FM Calibration Overview: The SAM FM calibration in the Mars chamber employed a variety of pure gases, gas mixtures, and solid materials. Isotope calibration runs for the TLS utilized 13C enriched C02 standards and 0 enriched CH4. A variety of fluorocarbon compounds that spanned the entire mass range of the QMS as well as C3-C6 hydrocarbons were utilized for calibration of the GCMS. Solid samples consisting of a mixture of calcite, melanterite, and inert silica glass either doped or not with fluorocarbons were introduced into the SAM FM cups through the SAM inlet funnel/tube system.

Mahaffy, Paul R.↗

DOE OSTI2024

Characterization and Optimization of Skipper CCDs for the SOAR Integral Field Spectrograph

We present results from the characterization and optimization of Skipper charge-coupled devices (CCDs) for use in a focal plane prototype for the Southern Astrophysical Research Integral Field Spectrograph (SIFS). We tested eight Skipper CCDs and selected six for SIFS based on performance results. The Skipper CCDs are 6k × 1k, 15 μm pixels, thick, fully depleted, p-channel devices that have been thinned to ~250 μm, backside processed, and treated with an anti-reflective coating. We demonstrate a single-sample readout noise of <4.3 e - rms pixel -1 in all amplifiers. We optimize the readout sequence timing to achieve a readout noise of 0.5 e - rms pixel -1 after 74 non-destructive measurements, which can be accomplished in a region covering 5% of the detector area in a readout time of <4 minutes. We demonstrate single-photon-counting in all 24 amplifiers (four amplifiers per detector) with a readnoise of σ N ~ 0.18 e - rms pixel -1 after N samp = 400 samples, and we constrain the degree of nonlinearity to be ≲1% at low signal levels (0 e - to 50 e - ). Clock-induced charge (CIC) remains an important issue when the Skipper CCD is configured to provide a large full-well capacity. We achieve a CIC rate of <1.45 × 10 -3 e - pixel -1 frame -1 for a full-well capacity of ~900 e - , which increases to a CIC rate of ~3 e - pixel -1 frame -1 for full-well capacities ~40,000-65,000 e - . We also perform conventional CCD characterization measurements such as charge transfer inefficiency (3.44 × 10 -7 on average), dark current (~2 × 10 -4 e - pixel -1 s -1 ), photon transfer curves, cosmetic defects (<0.45% “bad” pixels), and charge diffusion (point-spread function < 7.5 μm) to verify that these properties are consistent with expectations from conventional p-channel CCDs used for astronomy. Furthermore, we provide the first measurements of the brighter-fatter effect and absolute quantum efficiency (≳80% between 450 and 980 nm; ≳90% between 600 and 900 nm) using Skipper CCDs.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

DOE OSTI2024

Spirochaete genome identified in red abalone sample represents a novel genus Candidatus Haliotispira gen. nov. within the order Spirochaetales

A fully assembled spirochaete genome was identified as a contaminating scaffold in our red abalone ( Haliotis rufescens ) genome assembly. In this paper, we describe the analysis of this bacterial genome. The assembled spirochaete genome is 3.25 Mb in size with 48.5 mol% G+C content. The proteomes of 38 species were compared with the spirochaete genome and it was discovered to form an independent branch within the family Spirochaetaceae on the phylogenetic tree. The comparison of 16S rRNA sequences and average nucleotide identity scores between the spirochaete genome with known species of different families in Spirochaetia indicate that it is an unknown species. Further, the percentage of conserved proteins compared to neighbouring taxa confirm that it does not belong to a known genus within Spirochaetaceae . We propose the name Candidatus Haliotispira prima gen. nov., sp. nov. based on its taxonomic placement and origin. We also tested for the presence of this species in different species of abalone and found that it is also present in white abalone ( Haliotis sorenseni ). In addition, we highlight the need for better classification of taxa within the class Spirochaetia .

Microbiology↗

DOE OSTI2022

Primer for OTERR Core Reloading Optimization

OTERR (Optimization of Test Reactor Reloading) is a software tool which assists in finding optimal fuel assembly reloading patterns for nuclear reactors. It was created specifically to support the Versatile Test Reactor (VTR) program, but its functionality is general enough to be applied to most fast spectrum reactors which use hexagonal prismatic fuel elements. This document is meant to be a primer for new users of OTERR to walk through example workflows for core reloading optimization cases. Simple cases are followed step-by-step to discuss what needs to be done to complete a reloading optimization sequence. Note that the intent of this document is to provide practical examples for users to follow along with so they can quickly start using OTERR and then make changes to fit their own modeling needs. Detail is limited in terms of addressing additional features not used in these examples and especially lacking in discussion of theory used in the code. To better address these points, users are highly encouraged to refer to the OTERR User Manual and the OTERR Theory Manual.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

DOE OSTI2022

Block-structured, equal-workload, multi-grid-nesting interface for the Boussinesq wave model FUNWAVE-TVD (Total Variation Diminishing)

Abstract. We describe the development of a block-structured, equal-CPU-load (central processing unit), multi-grid-nesting interface for the Boussinesq wave model FUNWAVE-TVD (Fully Nonlinear Boussinesq Wave Model with Total Variation Diminishing Solver). The new model framework does not interfere with the core solver, and thus the core program, FUNWAVE-TVD, is still a standalone model used for a single grid. The nesting interface manages the time sequencing and two-way nesting processes between the parent grid and child grid with grid refinement in a hierarchical manner. Workload balance in the MPI-based (message passing interface) parallelization is handled by an equal-load scheme. A strategy of shared array allocation is applied for data management that allows for a large number of nested grids without creating additional memory allocations. Four model tests are conducted to verify the nesting algorithm with assessments of model accuracy and the robustness in the application in modeling transoceanic tsunamis and coastal effects.

Choi, Young-Kwang↗

NASA NTRS1971

Geophysical aspects of remote sensing

Results obtained through the NASA Earth Resources Aircraft Program at Mill Creek, Oklahoma, provide a case history example of the application of remote sensing to the identification of geologic rock units. Thermal infrared images are interpreted by means of a sequence of models of increasing complexity. The roles of various parameters are examined: rock properties (thermal inertia, albedo, emissivity), site location (latitude), season (sun's declination), atmospheric effects (cloud cover, transmission, air temperature), and topographic orientation (slope, azimuth). The results obtained at this site also illustrate the development of an important application of remote sensing in geologic identification. Relatively pure limestones and dolomites of the Mill Creek test area can be differentiated in nighttime infrared images, and facies changes between them can be detected along and across strike. The predominance on the earth's surface of sedimentary rocks, of which limestone and dolomite are major members, indicates the importance of this discrimination.

Watson, K.↗

NASA NTRS1973

Proposal for hierarchical description of software systems

The programming of digital computers has developed into a new dimension full of diffculties, because the hardware of computers has become so powerful that more complex applications are entrusted to computers. The costs of software development, verification, and maintenance are outpacing those of the hardware and the trend is toward futher increase of sophistication of application of computers and consequently of sophistication of software. To obtain better visibility into software systems and to improve the structure of software systems for better tests, verification, and maintenance, a clear, but rigorous description and documentation of software is needed. The purpose of the report is to extend the present methods in order to obtain a documentation that better reflects the interplay between the various components and functions of a software system at different levels of detail without losing the precision in expression. This is done by the use of block diagrams, sequence diagrams, and cross-reference charts. In the appendices, examples from an actual large sofware system, i.e. the Marshall System for Aerospace Systems Simulation (MARSYAS), are presented. The proposed documentation structure is compatible to automation of updating significant portions of the documentation for better software change control.

Thauboth, H.↗

NASA NTRS1991

Time-lapse CCD imagery of plasma-tail motions in Comet Austin

The appearance of the bright comet Austin 1989c1 in April-May of 1990 allowed us to test a new imaging instrument at the Joint Observatory for Cometary Research (JOCR). It is a 300mm lens/charge coupled device (CCD) system with interference filters appropriate for cometary emissions. The 13 frames were made into a time-lapse movie showing the evolution of the plasma tail. We were able to follow at least two large-scale waves out through the main tail structure. During the sequence, we saw two new tail rays form and undergo similar wave motion.

Klinglesmith, Daniel A., III↗

NASA NTRS1970

HELIOS Third Joint Working Group Meeting

During the past six months since the Second Helios Joint Working Group Meeting held 27-30 April 1970 at Goddard Space Flight Center, the TDS Sub-Group supported the Helios Project Office and the other Sub-Groups in the timely disposition of action items and the dissemination of information pertinent to the development of interface documentation. Of particular importance during this time period was the Project's decision to incorporate a single-channel telemetry system design aboard the spacecraft. The TDS Sub-Group participated actively in the process that led to this decision. Still under active study with TDS participation is the pending Project Office decision regarding the incorporation of a ranging capability within the telecommunications design. The TDS Sub-Group assisted the Mission Analysis and Operations Sub-Group in establishment of a study effort concerning the Near-Earth Sequence of Events from launch to launch plus 8 hours. This study, which will provide valuable data for the spacecraft telecommunications design, will include participation by the Experiment, Launch Vehicle, Spacecraft, as well as the TDS and MA&O Sub-Groups. Also during the past 6-month period, the TDS, in conjunction with the Spacecraft Sub-Group, initiated activity to develop the Helios Spacecraft/TDS Compatibility Test Plans and Procedures. Activity concerning the foregoing interface discussions has been and will continue to be based upon the "TDS Estimated Capabilities Document for the Helios Missions" (613-1), and the "DSN/Flight Project Interface Design Handbook" (810-5). These will continue to be considered TDS controlling documents until specific Helios Project/TDS interface documentation is generated and signed off by the respective parties. In addition to the above, the DSN continued the Helios Trainee Program with seven GfW/DFVLR trainees in residence at JPL. Two trainees will complete their year's residency concurrent with the Third Helios Joint Working Group Meeting, while four new trainees are expected to arrive following the Third Helios Joint Working Group Meeting. These and other activities are reported in detail in the paragraphs that follow.

Ousley, Gilbert↗