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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 1,747 records · Page 97
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Dark matter axion search using a Josephson Traveling wave parametric amplifier

Here, we describe the first implementation of a Josephson Traveling Wave Parametric Amplifier (JTWPA) in an axion dark matter search. The operation of the JTWPA for a period of about two weeks achieved sensitivity to axion-like particle dark matter with axion–photon couplings above 10 -13 Ge V -1 over a narrow range of axion masses centered around 19.84 µeV by tuning the resonant frequency of the cavity over the frequency range of 4796.7–4799.5 MHz. The JTWPA was operated in the insert of the axion dark matter experiment as part of an independent receiver chain that was attached to a 0.56-l cavity. The ability of the JTWPA to deliver high gain over a wide (3 GHz) bandwidth has engendered interest from those aiming to perform broadband axion searches, a longstanding goal in this field.

47 OTHER INSTRUMENTATION↗
DOE OSTI2024

Design of AlGaN-Zn(Si,Ge)N2 quantum wells for high-efficiency ultraviolet light emitters

The effect of inserting a nm-scale layer of Zn(Si,Ge)N2 into an AlGaN quantum well structure designed for light emission in the wavelength range from 255 to 305 nm is investigated here. The enhanced confinement of the hole within the quantum well results in an enhancement of the overlap of the hole and electron wave functions, resulting in an enhancement of the radiative recombination rate. In this theoretical calculation, for emission at a 270 nm wavelength, the enhancement in the wavefunction overlap can reach a factor of 7 when compared to an AlGaN quantum well device specifically engineered for optimal emission at the identical wavelength. Increases of almost an order of magnitude in both the peak spontaneous emission intensity and the radiative recombination rate are predicted. The peak emission wavelength can be tuned from 255 to 305 nm by adjusting the width and/or the composition of the inserted layer. The proposed structures provide a route to higher efficiency ultraviolet practical light emitting diodes and lasers.

Physics↗
DOE OSTI2011

All-optical loadable and erasable memory cell design based on inversionless lasing and electromagnetically induced transparency effects

A loadable and erasable all-optical memory cell is designed by using two coupled micro-ring resonators with electromagnetically induced transparency (EIT) and lasing without inversion (LWI). To read out stored data, an additional phase is introduced in the upper ring resonator due to EIT. To compensate the fibre loss, use is made of LWI. The EIT is induced by inserting {Lambda}-type three level quantum dots in the right-hand half of the upper ring and LWI is implemented by inserted Y-type four level quantum dots in the left-hand half of both rings. This optical memory cell can operate at a low light power level corresponding to several photons.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2013

Transmission spectra of a double-clad fibre structure under bending

We have studied a fibre-optic structure whose operation relies on conversion of core and cladding modes that are coupled across the interface between two fibres differing in refractive index profile. The structure contains a section of an SM630 double-clad, small-core, single-mode fibre inserted between two SMF-28 standard fibres. We have measured the transmission spectrum of the structure when the SM630 fibre was bent and analysed the mode structure of the double-clad fibre and the origin of dips in its transmission spectrum. The resonance dips have been found to shift to longer wavelengths with increasing fibre curvature. We have evaluated the shift as a function of the length of the inserted fibre, its bend direction and the nature of the input fibre. (fiber optics)

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2011

Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin

Seasonal antigenic drift of circulating influenza virus leads to a requirement for frequent changes in vaccine composition, because exposure or vaccination elicits human antibodies with limited cross-neutralization of drifted strains. We describe a human monoclonal antibody, CH65, obtained by isolating rearranged heavy- and light-chain genes from sorted single plasma cells, coming from a subject immunized with the 2007 trivalent influenza vaccine. The crystal structure of a complex of the hemagglutinin (HA) from H1N1 strain A/Solomon Islands/3/2006 with the Fab of CH65 shows that the tip of the CH65 heavy-chain complementarity determining region 3 (CDR3) inserts into the receptor binding pocket on HA1, mimicking in many respects the interaction of the physiological receptor, sialic acid. CH65 neutralizes infectivity of 30 out of 36 H1N1 strains tested. The resistant strains have a single-residue insertion near the rim of the sialic-acid pocket. We conclude that broad neutralization of influenza virus can be achieved by antibodies with contacts that mimic those of the receptor.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2017

Cas9-mediated genome editing in the methanogenic archaeon Methanosarcina acetivorans

Although Cas9-mediated genome editing has proven to be a powerful genetic tool in eukaryotes, its application in Bacteria has been limited because of inefficient targeting or repair; and its application to Archaea has yet to be reported. Here we describe the development of a Cas9-mediated genome-editing tool that allows facile genetic manipulation of the slow-growing methanogenic archaeon Methanosarcina acetivorans . Introduction of both insertions and deletions by homology-directed repair was remarkably efficient and precise, occurring at a frequency of approximately 20% relative to the transformation efficiency, with the desired mutation being found in essentially all transformants examined. Off-target activity was not observed. We also observed that multiple single-guide RNAs could be expressed in the same transcript, reducing the size of mutagenic plasmids and simultaneously simplifying their design. Cas9-mediated genome editing reduces the time needed to construct mutants by more than half (3 vs. 8 wk) and allows simultaneous construction of double mutants with high efficiency, exponentially decreasing the time needed for complex strain constructions. Furthermore, coexpression the nonhomologous end-joining (NHEJ) machinery from the closely related archaeon, Methanocella paludicola , allowed efficient Cas9-mediated genome editing without the need for a repair template. The NHEJ-dependent mutations included deletions ranging from 75 to 2.7 kb in length, most of which appear to have occurred at regions of naturally occurring microhomology. The combination of homology-directed repair-dependent and NHEJ-dependent genome-editing tools comprises a powerful genetic system that enables facile insertion and deletion of genes, rational modification of gene expression, and testing of gene essentiality.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2019

Crystal structure and activity-based labeling reveal the mechanisms for linkage-specific substrate recognition by deubiquitinase USP9X

USP9X is a conserved deubiquitinase (DUB) that regulates multiple cellular processes. Dysregulation of USP9X has been linked to cancers and X-linked intellectual disability. Here, we report the crystal structure of the USP9X catalytic domain at 2.5-Å resolution. The structure reveals a canonical USP-fold comprised of fingers, palm, and thumb subdomains, as well as an unusual β-hairpin insertion. The catalytic triad of USP9X is aligned in an active configuration. USP9X is exclusively active against ubiquitin (Ub) but not Ub-like modifiers. Cleavage assays with di-, tri-, and tetraUb chains show that the USP9X catalytic domain has a clear preference for K11-, followed by K63-, K48-, and K6-linked polyUb chains. Using a set of activity-based diUb and triUb probes (ABPs), we demonstrate that the USP9X catalytic domain has an exo-cleavage preference for K48- and endo-cleavage preference for K11-linked polyUb chains. The structure model and biochemical data suggest that the USP9X catalytic domain harbors three Ub binding sites, and a zinc finger in the fingers subdomain and the β-hairpin insertion both play important roles in polyUb chain processing and linkage specificity. Furthermore, unexpected labeling of a secondary, noncatalytic cysteine located on a blocking loop adjacent to the catalytic site by K11-diUb ABP implicates a previously unreported mechanism of polyUb chain recognition. The structural features of USP9X revealed in our study are critical for understanding its DUB activity. The new Ub-based ABPs form a set of valuable tools to understand polyUb chain processing by the cysteine protease class of DUBs.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2019

A comprehensive genomic scan reveals gene dosage balance impacts on quantitative traits in Populus trees

Gene dosage variation and the associated changes in gene expression influence a wide variety of traits, ranging from cancer in humans to yield in plants. It is also expected to affect important traits of ecological and agronomic importance in forest trees, but this variation has not been systematically characterized or exploited. Here we performed a comprehensive scan of the Populus genome for dosage-sensitive loci affecting quantitative trait variation for spring and fall phenology and biomass production. The study population was a large collection of clonally propagated F1 hybrid lines of Populus that saturate the genome 10-fold with deletions and insertions (indels) of known sizes and positions. As a group, the phenotypic means of the indel lines consistently differed from control nonindel lines, with an overall negative effect of both insertions and deletions on all biomass-related traits but more diverse effects and an overall wider phenotypic distribution of the indel lines for the phenology-related traits. We also investigated the correlation between gene dosage at specific chromosomal locations and phenotype, to identify dosage quantitative trait loci (dQTL). Such dQTL were detected for most phenotypes examined, but stronger effect dQTL were identified for the phenology-related traits than for the biomass traits. Furthermore our genome-wide screen for dosage sensitivity in a higher eukaryote demonstrates the importance of global genomic balance and the impact of dosage on life history traits.

09 BIOMASS FUELS↗
DOE OSTI2022

Pseudouridine synthase 7 is an opportunistic enzyme that binds and modifies substrates with diverse sequences and structures

Pseudouridine (Ψ) is a ubiquitous RNA modification incorporated by pseudouridine synthase (Pus) enzymes into hundreds of noncoding and protein-coding RNA substrates. Here, in this study, we determined the contributions of substrate structure and protein sequence to binding and catalysis by pseudouridine synthase 7 (Pus7), one of the principal messenger RNA (mRNA) modifying enzymes. Pus7 is distinct among the eukaryotic Pus proteins because it modifies a wider variety of substrates and shares limited homology with other Pus family members. We solved the crystal structure of Saccharomyces cerevisiae Pus7, detailing the architecture of the eukaryotic-specific insertions thought to be responsible for the expanded substrate scope of Pus7. Additionally, we identified an insertion domain in the protein that fine-tunes Pus7 activity both in vitro and in cells. These data demonstrate that Pus7 preferentially binds substrates possessing the previously identified UGUAR (R = purine) consensus sequence and that RNA secondary structure is not a strong requirement for Pus7-binding. In contrast, the rate constants and extent of Ψ incorporation are more influenced by RNA structure, with Pus7 modifying UGUAR sequences in less-structured contexts more efficiently both in vitro and in cells. Although less-structured substrates were preferred, Pus7 fully modified every transfer RNA, mRNA, and nonnatural RNA containing the consensus recognition sequence that we tested. Our findings suggest that Pus7 is a promiscuous enzyme and lead us to propose that factors beyond inherent enzyme properties (e.g., enzyme localization, RNA structure, and competition with other RNA-binding proteins) largely dictate Pus7 substrate selection.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2011

The Structure of a High Fidelity DNA Polymerase Bound to a Mismatched Nucleotide Reveals an ;Ajar; Intermediate Conformation in the Nucleotide Selection Mechanism

To achieve accurate DNA synthesis, DNA polymerases must rapidly sample and discriminate against incorrect nucleotides. Here we report the crystal structure of a high fidelity DNA polymerase I bound to DNA primer-template caught in the act of binding a mismatched (dG:dTTP) nucleoside triphosphate. The polymerase adopts a conformation in between the previously established 'open' and 'closed' states. In this 'ajar' conformation, the template base has moved into the insertion site but misaligns an incorrect nucleotide relative to the primer terminus. The displacement of a conserved active site tyrosine in the insertion site by the template base is accommodated by a distinctive kink in the polymerase O helix, resulting in a partially open ternary complex. We suggest that the ajar conformation allows the template to probe incoming nucleotides for complementarity before closure of the enzyme around the substrate. Based on solution fluorescence, kinetics, and crystallographic analyses of wild-type and mutant polymerases reported here, we present a three-state reaction pathway in which nucleotides either pass through this intermediate conformation to the closed conformation and catalysis or are misaligned within the intermediate, leading to destabilization of the closed conformation.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Computational Thermal Hydraulics of a High-Performance Low-Enriched-Uranium Annular Target for HFIR Irradiation

Molybdenum-99 has historically been generated via isolation from fissioned highly enriched uranium (HEU) targets. Here, this isotope is in high demand due to its daily use across the world in radiopharmaceutical medical procedures. The primary objective of this work was to design and analyze an experimental target assembly containing one low-enriched-uranium (LEU) annular target for irradiation at the High Flux Isotope Reactor (HFIR). Efforts included incorporating spatially dependent energy sources from neutron and gamma interactions, quantifying thermal contact conductance at material interfaces, performing grid-independent studies, comparing turbulence models, and simulating various steady-state and transient scenarios relevant for irradiation qualification and eventual insertion. These models provide velocity, pressure, and temperature distributions in both space and time. Such results enable the selection of an appropriate irradiation location, fission rate density, and flow-limiting orifice size and demonstrate compliance with HFIR safety requirements such that insertion into the reactor can be approved. This analysis shows that across all scenarios, wetted surface temperatures remain below the coolant saturation temperature with no net vapor formation in the coolant. In every scenario, all components stay below 30% of the aluminum 6061 melting temperature. Computational fluid dynamics and system-level models predict peak target temperatures that agree within 4%, though the predicted axial location of the peak differs by about 10% of the heated length due to differences in flow development length. These results de-risk the irradiation of LEU (annular targets) and strengthen a domestic, HEU-independent 99 Mo supply by providing important fuel performance data to form the foundation for a robust licensing basis.

Molybdenum-99↗
DOE OSTI2023

Concept Descriptions for the VTR Rabbit System and Driver Fuel Test Assemblies

Two of the experiment vehicles being developed for the Versatile Test Reactor (VTR) are presented here. The first is a rabbit system that will enable rapid insertion of small test capsules into the high fast flux of the VTR core for relatively short durations. The rabbit concept development includes the construction/demonstration of a near-full-scale system in a deep-water pool to demonstrate functionality, development of a concept of operations and initial procedures, and validation of thermal-hydraulic modeling. In addition, modeling efforts are underway to simulate the thermal and neutronic environment of a rabbit capsule. The second type of experiment vehicle presented here is a driver fuel test assembly for inserting fuel and materials tests into the core by replacing a driver fuel assembly. Here, a novel design for dismountable test assemblies is proposed for the VTR.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2017

Evaluation of Direct Drive Capsule Fill-Tube Assembly Survivability in Support of the 100 GBar Campaign

Capsule fill-tube target assemblies (CFTAs) for direct-drive have been demonstrated in the past for Laser for Laboratory Energetics (LLE) cryogenic layering studies, evolving and building upon successful deliveries for NIF. The current 100 GigaBar (GBar) campaign requires increased tolerances over prior CFTA work in terms of robustness, cleanliness, reduced fill tube diameter and glue size. To tackle these challenges, GA and LLE are developing a direct drive CFTA that survives all fabrication activities, from assembly, qualification testing and transport to cryogenic layering and target chamber insertion at the OMEGA laser facility. Approximately 55 CFTAs of two main designs have been constructed and tested to date, building off the current LLE cryogenic layering study CFTA design (typically 870 µm diameter x 25 µm thick wall capsule). Variations of glass fill tubes sizes from 30, 20 and 10 µm diameter, which are inserted and glued into the capsule. Testing protocols were developed to enable comparison of different designs against one another and evaluate their robustness at room temperature. The testing protocols include pressure checks, resonant vibration mode sweeps, runout and vibration testing to a power spectral density (PSD) input. In this paper we will compare the different design tradeoffs, measurements and results including glue fillets, sag of the fill tubes under gravity, runout and hysteresis, ringdown response, X-ray imaging of the pipette wall thicknesses, and scanning electron microscope (SEM) images taken of failed fill tubes and glue joints. Assessment of the modal vibration modeling for the designs will be compared against the vibration data. Lastly, a design recommendation is put forth meeting the design constraints, which consists of a polymicro composite tube and 10 µm fill tube, ensuring survivability at cryogenic temperatue, a higher first mode resonance and smaller fuel volume.

100 GBar↗
DOE OSTI2011

MAGNETOHYDRODYNAMIC SIMULATIONS OF RECONNECTION AND PARTICLE ACCELERATION: THREE-DIMENSIONAL EFFECTS

Magnetic fields can change their topology through a process known as magnetic reconnection. This process in not only important for understanding the origin and evolution of the large-scale magnetic field, but is seen as a possibly efficient particle accelerator producing cosmic rays mainly through the first-order Fermi process. In this work we study the properties of particle acceleration inserted in reconnection zones and show that the velocity component parallel to the magnetic field of test particles inserted in magnetohydrodynamic (MHD) domains of reconnection without including kinetic effects, such as pressure anisotropy, the Hall term, or anomalous effects, increases exponentially. Also, the acceleration of the perpendicular component is always possible in such models. We find that within contracting magnetic islands or current sheets the particles accelerate predominantly through the first-order Fermi process, as previously described, while outside the current sheets and islands the particles experience mostly drift acceleration due to magnetic field gradients. Considering two-dimensional MHD models without a guide field, we find that the parallel acceleration stops at some level. This saturation effect is, however, removed in the presence of an out-of-plane guide field or in three-dimensional models. Therefore, we stress the importance of the guide field and fully three-dimensional studies for a complete understanding of the process of particle acceleration in astrophysical reconnection environments.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2011

Small animal simultaneous PET/MRI: initial experiences in a 9.4T microMRI

We developed a non-magnetic positron-emission tomography (PET) device based on the rat conscious animal PET that operates in a small-animal magnetic resonance imaging (MRI) scanner, thereby enabling us to carry out simultaneous PET/MRI studies. The PET detector comprises 12 detector blocks, each being a 4 x 8 array of lutetium oxyorthosilicate crystals (2.22 x 2.22 x 5 mm{sup 3}) coupled to a matching non-magnetic avalanche photodiode array. The detector blocks, housed in a plastic case, form a 38 mm inner diameter ring with an 18 mm axial extent. Custom-built MRI coils fit inside the positron-emission tomography (PET) device, operating in transceiver mode. The PET insert is integrated with a Bruker 9.4 T 210 mm clear-bore diameter MRI scanner. We acquired simultaneous PET/MR images of phantoms, of in vivo rat brain, and of cardiac-gated mouse heart using [{sup 11}C]raclopride and 2-deoxy-2-[{sup 18}F]fluoro-d-glucose PET radiotracers. There was minor interference between the PET electronics and the MRI during simultaneous operation, and small effects on the signal-to-noise ratio in the MR images in the presence of the PET, but no noticeable visual artifacts. Gradient echo and high-duty-cycle spin echo radio frequency (RF) pulses resulted in a 7% and a 28% loss in PET counts, respectively, due to high PET counts during the RF pulses that had to be gated out. The calibration of the activity concentration of PET data during MR pulsing is reproducible within less than 6%. Our initial results demonstrate the feasibility of performing simultaneous PET and MRI studies in adult rats and mice using the same PET insert in a small-bore 9.4 T MRI.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2023

Soft particles and infinite-dimensional geometry

Abstract In the sigma model, soft insertions of moduli scalars enact parallel transport ofS-matrix elements about the finite-dimensional moduli space of vacua, and the antisymmetric double-soft theorem calculates the curvature of the vacuum manifold. We explore the analogs of these statements in gauge theory and gravity in asymptotically flat spacetimes, where the relevant moduli spaces are infinite-dimensional. These models have spaces of vacua parameterized by (trivial) flat connections on the celestial sphere, and soft insertions of photons, gluons, and gravitons parallel transportS-matrix elements about these infinite-dimensional manifolds. We argue that the antisymmetric double-soft gluon theorem ind + 2 bulk dimensions computes the curvature of a connection on the infinite-dimensional space Map ( S d , G ) / G , whereGis the global part of the gauge group. The analogous metrics in abelian gauge theory and gravity are flat, as indicated by the vanishing of the antisymmetric double-soft theorems in those models. In other words, Feynman diagram calculations not only know about the vacuum manifold of Yang–Mills theory, they can also be used to compute its curvature. The results have interesting implications for flat space holography.

Astronomy & Astrophysics↗
DOE OSTI2025

Magnetic and Mechanical Analysis of Bi-2212 Rutherford Cable in a Cos-Theta Sub-Scale Dipole Coil

The U.S. Magnet Development Program (US-MDP) explores high-field accelerator magnets compatible with operational conditions beyond the limits of Nb$_3$Sn technology. The ongoing R&D High-Temperature Superconductors (HTS) suggests using Bi$_2$Sr$_2$CaCu$_2$O$_{8-x}$ (Bi-2212) as superconducting element. Bi-2212 Rutherford cables maintain a high critical current (I$_C$) when exposed to a large external magnetic field. However, Bi-2212 exhibits an oversensitive stress-strain response when subject to large Lorentz forces. This paper reports on the magnetic and mechanical analysis of the Bi-2212 cosine-theta insert being developed at Fermilab for a hybrid magnet composed of two external layers of Nb$_3$Sn and two internal layers of Bi-2212. We performed a FEM analysis of the insert to estimate the HTS stress state in the coil's strands under magnetic and mechanical loads.

43 PARTICLE ACCELERATORS↗
DOE OSTI2017

Tungsten erosion by unipolar arcing in DIII-D

Here, unipolar arcing was an important mechanism of metal surface erosion during the recently conducted Metal Rings Campaign in DIII-D when two toroidally continuous tile rings with 5 cm wide W-coated TZM inserts were installed in graphite tiles in the lower divertor, one on the floor and one on the shelf. Most of the arc damage occurred on the shelf ring. The total amount of W removed by arcing from the affected ~4% of the shelf ring area was estimated ~0.8 × 10 21 at., about half of the total amount of W eroded and redeposited outside the inserts (1.8 ± 0.9)×10 21 at. The rings were exposed for a total of ~480 discharges, an equivalent of plasma time on W surfaces (with ${{I}}_{{\rm{p}}}\gt 0.5$ MA) ~10 3 s. Arcing was monitored in situ with WI (400.9 nm) filtered camera and photomultipliers and showed that: (i) arcing only occurred during ELMs and disruptions, (ii) arcing rate was much lower on the floor than on the shelf ring, and (iii) arcing had a low cut off power flux density about 2 MW m –2 . About half of arc tracks had large ${10}^{\circ }$ pitch angle and probably were produced during disruptions. Such tracks were only found on the shelf. Moderate toroidal variation of the arc track density and W erosion with nearly n = 1 pattern has been measured.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗