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At least 1,711 records · Page 95

Post-Flight Assessment Review (PFAR): Advanced Colloids Experiment (ACE) ACE-T7-1,2

The objective of this PFAR is to: • Provide closure to ZIN DO-237 ACE-T7work and close NASA NPR 7123.1 requirements as listed in the SpaceDOC-II (S2) contract (the S2 CDRL DID No. PM-09 calls for a SEMP which refers backto NPR 7123.1) • Provide closure of SEMP (ZIN’s P40025) requirements for Technical Reviews • Provide plan to complete Informatics requirements. • Provide milestone closure to sponsoring organization. • Document property re-use and transfer decisions. • Transfer raw science data to the designated repository at MSFC. • Transfer required documents to Informatics • Opens an Informatics folder at MSFC to officially receive ACE-T7 information. • Capture open action items. • Create a formal presentation in configuration control. • Provide directions (in a Project Manager Report) how to contact the Project and Science teams through NASA when follow-on grants are awarded by Informatics.

microgravity↗

Design of an Excavation Robot: Regolith Advanced Surface Systems Operations Robot (RASSOR) 2.0

To continue on a sustainable and flexible path, NASA needs to address the challenge of collecting and moving large amounts of regolith at the destination. Acquiring the water resources on Mars will require mining significant quantities of regolith and this is not possible with the state-of-the-art low mass excavation systems. Low gravity environments (Mars = 3/8 G) and launch mass restrictions limit the traction and the resulting reaction force of the vehicle, making current terrestrial techniques impractical. This project addressed this challenge by developing a completely new technology that can mine large quantities of regolith on Mars. Recent measurements by the “Curiosity” rover on Mars have found that the regolith contains ~ 2% water by weight globally, ~4% in Jezero Crater (Human Architecture Team’s reference landing site), and much more at the poles(Leshin et al, 2013). RASSOR 2.0 is a planetary excavator, which has a mass of 66 kg, with a 0.38 kg vehicle mass per kilogram, per hour of excavation rate and power usage of 4 W per kg of regolith excavation rate. A single RASSOR 2.0 can excavate a minimum of 2.7 metric tons of regolith per day.This is accomplished by using counteracting excavation forces on two opposing digging implements called bucket drums and an autonomous mining control system. This work has addressed several major research areas outlined in the NASA Technology Area (TA) 04 Robotics & Autonomous Systems and TA 07 Human Destination Systems roadmaps. This project started at Technology Readiness Level (TRL) 4 as a low fidelity “proof of concept” prototype which has successfully demonstrated basic regolith simulant excavation functionality in a lab-scale gravity off load test. The foundational technology described here was awarded US patent number: US 9027265 for a “Zero horizontal reaction force excavator” on May 12, 2015.

RASSOR↗

Probabilistic Modeling of a Three-Stage Human Landing System Architecture

Space Policy Directive-1 has led to NASA partnerships with commercial entities on procurement which includes the development of the Human Landing System (HLS) [1]. With the goal of delivering human crew to the lunar surface by 2024, system uncertainties become an important obstacle to the maturation of multiple new, driving technologies and mission concepts of the HLS program. As unmitigated uncertainties have previously led to failed development programs, these risks and their impacts must be understood and handled to ensure program success [2]. Sources of uncertainty include novel engine designs and configurations, increased reliance on cryogenic fluid management(CFM), and refueling technologies—which propagate as high-level performance metrics such as overall propellant mass and engine performance. Also, the occurrence of operational uncertainties—e.g. launch conditions or need to abort during the mission—can cause cascading effects on the rest of the mission that are difficult to definitively quantify, and are outside the scope of control. These concrete examples and other occurrences can be categorized as either epistemic or aleatory uncertainties.Epistemic uncertainty arises due to a lack of knowledge and can be alleviated with design and program maturation. Aleatory uncertainty is due to the inherent randomness of the system and cannot be directly reduced, unlike epistemic uncertainty. Robust design and probabilistic methods can compensate for aleatory effects. A taxonomy of uncertainty is referred to for this work [3]. In this paper, a probabilistic methodology to handle uncertainties has been demonstrated on a three-element HLS concept [1, 4], which allows tracking of current best estimates of the concept and assessment of concept design robustness against uncertainties. A sample case has been completed for this abstract, and an expansion on the methodology will be included in the final paper. This methodology has two key parts: first, the creation of a dynamic architecture model of a three-element HLS concept; and second, its use with surrogate modeling and range estimating techniques to capture and propagate uncertainties. This abstract will cover the basics of the approach used, and further details and justifications will be in the final paper.The mission profile associated with this three-element concept (Fig 1) was modeled as a set of mission events that facilitated mass changes, idles, or spacecraft maneuvers. The mission profile scope starts with each element’s NRHO orbit insertion and aggregation and ends at post-sortie rendezvous with Orion. More detail on the mission profile will be in the final paper. The DYnamic Rocket EQuation Tool (DYREQT), a space systems synthesis and sizing framework used by NASA, was used as the physics framework to model the HLS architecture for applying the probabilistic methodology [5, 6]. Specifically, a parametric representation of the lander, ascent, and transfer elements and the mission profile of each element was established, with vehicle and mission parameters available as inputs to allow for a dynamic model. Each vehicle stage was modeled with high-level performance metrics, using Isp and propellant mass fraction (PMF) to remain parametric. For the probabilistic analysis, uncertainties of interest within the HLS concept were enumerated and represented as parameters within the DYREQT model as inputs for vehicle stages or mission profile events. These parameters were frozen at their nominal values for the purposes of baselining architecture performance and sizing the vehicle appropriately based on reference documentation [1]. Range estimating—a probabilistic method that combines Monte Carlo sampling, focus on critical parameters, and heuristics to assess risk and opportunities—is traditionally used with Mass Equipment Lists (MELs), but has been adapted with operational parameters as well as vehicle parameters in theDYREQT model to capture mission uncertainty alongside vehicle uncertainty [7, 3]. This method was selected due to its application and insight on a system from a bottom-up perspective, independence from historical rules of thumb, and ability to generate sensitivities based on design decisions and uncertainties. As a sample case for the abstract, the boiloff rates of the vehicle elements and the loiter times during the mission (simulating launch time variations and changing window of opportunities) were used with range estimating to provide preliminary results. To perform the range estimation portion of this methodology (depicted in Fig. 3, further details in final paper), the DYREQT model was sampled using a Design of Experiments (DoE) to efficiently explore the architecture design space with respect to the sample set of uncertainty parameters; 5,000 cases via Latin Hypercube Sampling were computed on the DYREQT architecture model. Then, the results were used to create surrogate models, multivariate regressions that can visualize hypercube trends in the design space, of the architecture with respect to the uncertainty parameters. Range estimating was applied to the surrogates instead of the actual models, which saves computational expense due to the bulk of cases needed for the Monte Carlo simulation as part of range estimating. Uncertainty parameters were sampled independently from triangular distributions using the DoE ranges as ‘min’ and ‘max’, and the nominal value as ‘most likely’. Based engineering intuition, some uncertainty parameters are correlated—e.g. if the main propellant has a high boil-off rate, the oxidizer should follow suit as both are related to CFM technology.While a Monte Carlo simulation samples all inputs as independent, the results would show model correlations; thus, it is efficient to sample the inputs as correlated. Using a correlation matrix constructed for the uncertainty parameters, previously independent samples were transformed to perform a Correlated Monte Carlo. A table for the DoE ranges and probability distribution parameters is shown in Table 1, and more details on Correlated Monte Carlo Simulations will be discussed in the final paper. The model’s resulting DoE showed that multivariate polynomial equations fit via least squares method captured its behavior accurately for the sample case. For the Correlated Monte Carlo Simulation, a positive correlation between fuel and oxidizer boiloff rates was used as a demonstration. 10,000 cases were computed with the surrogates and the launched masses for each vehicle element was collated. The results can be displayed in a probability density function (PDF), showing the impact of the uncertainty parameters chosen. Integrating the PDFs will yield a cumulative distribution function (CDF) that shows the cumulative probability of a given value on the x-axis. For the sample case, the elements’ launch mass margin was calculated and represented in as CDFs, as a demonstrated representation of figures of merit for the HLS concept. For the lander and ascent elements, the NRHO mass insertion limit is 16t; the transfer element has a limit of 30t [1]. It can be seen with Figure 2 that this probabilistic methodology can provide insight into mass margin with respect to the uncertainties being modeled. Currently, the results show that the lander (descent) vehicle element has the most restrictive design space; it is the only element to show a 10% probability of negative margin. Further analysis on the Monte Carlo results will show sensitivities for driving constraints and parameters for architecture feasibility, which can lead to establishing potential mission rules.The combination of range estimating with a parametric architecture model for HLS demonstrated the capability of this probabilistic methodology in a sample case. As the HLS development progresses, this methodology has the potential for keeping current best estimates of architecture performance for awarded concepts due to the flexibility in DYREQT’s modeling framework and its parametric nature. Concept maturation and increased epistemic knowledge can be injected into the model probabilistic modeling, and thus continue to track probability of mission success.

Stephanie Y Zhu↗

The Eris/Dysnomia System I: The Orbit of Dysnomia

We present new results on the Eris/Dysnomia system including analysis of new images from the WFC3 instru-ment on the Hubble Space Telescope (HST). Seven HST orbits were awarded to program 15171 in January and February 2018, with the intervals between observations selected to sample Dysnomia over a full orbital period. Using relative astrometry of Eris and Dysnomia, we computed a best-fit Keplerian orbit for Dysnomia. Based on the Keplerian fit, we find an orbital period of 15.785899±0.000050 days, which is in good agreement with recent work. We report a non-zero eccentricity of 0.0062 at the 6.2-σ level, despite an estimated eccentricity damping timescale of ≤17 Myr. Considering the volumes of both Eris and Dysnomia, the new system density was calculated to be 2.43±0.05 g cm3, a decrease of ~4% from the previous value of 2.52±0.05 g cm3. The new astrometric measurements were high enough precision to break the degeneracy of the orbit pole orientation, and indicate that Dysnomia orbits in a prograde manner. The obliquity of Dysnomia’s orbit pole with respect to the plane of Eris’ heliocentric orbit was calculated to be 78.29±0.65∘ and is in agreement with previous work; the next mutual events season will occur in 2239. The Keplerian orbit fit to all the data considered in this investi-gation can be excluded at the 6.3-σ level, but identifying the cause of the deviation was outside the scope of this work.

B J Holler↗

The Effects of Simulated Microgravity and PLacental-EXpanded (PLX-PAD) Stromal Cell Treatment on The Behavior and Correlation with Cytokine Profiles in Female Mice

Spacelight can lead to altered immune responses and inflammation (Crucian et al. 2014) and elevated levels of inflammation are connected to anxiety and depression. A recent study on the International Space Station showed that mice exhibited a novel circling behavior during spaceflight (Ronca 2019). However, there is still a gap in knowledge on how microgravity impacts behavior. In this current study, we performed 30 days of hindlimb unloading (HU) on four-month old female mice and analyzed select behavior from video image capture. We also determined the effects of PLacental-eXpanded stromal cells dervided from the maternal placenta (PLX-PAD), alone and in combination with HU and isolation, on behavior. We have previoulst shown that PLX-PAD mitigated select inflammatory responses and changes in cytokine expression causes by HU. In-cage behaviors were analyzed in HU or control female mice treated with 2 injections of PlasmaLyte (Sham) or PLX-PAD (n=7/group). We found that PLX-PAD decreased exploratory behaviors compared to Sham treated mice at night. Normally loaded (NL) PLX mice slept less than NL Sham mice during the day, and HU animals had signficantly higher involuntary movement during sleep compared to NL animals, suggesting sleep distruption. Overall, we show that both HU and PLX affect important behavioral factors., This experiment is the first to study the effects of PLX-PAD on behavior. Additional studies are needed to define the behavioral changes in spaceflight and test possible countermeasures. Funding for this project was provided through the 2019 Ames Research Innovation Award (ARIA) provided by NASA Ames Research Center.

Stromal cell treatment↗

GA-ASI Final Report and Program Wrap-up for NASA System Integration and Operationalization (SIO)

The NASA SIO demonstration flight of April 3, 2020 represents a successful culmination of 18 months of coordinated effort between GA-ASI, NASA, the FAA, Collins Aerospace, and Honeywell Aerospace to operate a Medium Altitude, Long Endurance (MALE) Unmanned Aircraft System (UAS) safely in the National Airspace System (NAS) using industry leading prototype technologies. The GA-ASI team consisted of technical experts, program managers, engineers, mechanics, flight technicians, flight crews, and numerous other subject matter experts. This final report describes the most significant and potentially impactful aspects of the planning, integration, test, and flight aspects of this effort. GA-ASI successfully integrated the key technologies needed for UAS to fly in the NAS onto our prototype SkyGuardian UAS, which was designed to meet the most stringent airworthiness standards applicable to an aircraft of its size category. A proven Detect and Avoid (DAA) system, developed by GA-ASI and utilizing Honeywell Aerospace technology was integrated onto SkyGuardian for the first time, along with datalink radios from Collins Aerospace that meet the new civil standard for Control and Non-Payload Communication (CNPC) links. GA-ASI also obtained approvals from the FAA and FCC to operate the reconfigured UAS. The SIO demonstration flight represented a commercial aerial surveying operation conducted at medium altitude (>10,000ft above mean sea level). The aircraft’s onboard sensors were used to capture photographic, infrared and radar imagery of public and commercial infrastructure and land, and to subsequently produce the types of data products that would provide business value to potential customers. These survey services would supplement or replace services currently provided by manned airplanes and helicopters, small drones or satellites. A “virtual” mission was also planned, to show what additional survey data could have been captured during the flight if additional sensors had been installed on the aircraft’s external hardpoints. This revision of the final report focuses on information of value to the wider UAS community, and avoids propriety data to facilitate broad dissemination. It also includes a description of GA-ASI’s engagement with the FAA following the SIO flight, which led to the award of an updated Special Airworthiness Certificate in the Experimental Category (SAC-EC) and a Certificate of Waiver or Authorization (COA) allowing operation of the SkyGuardian UAS using its DAA system to satisfy right-of-way rules, instead of a chase plane. The associated operational limitations are described to illustrate the further steps that would be needed to remove those limitations for unhindered commercial operations.

Flight demonstration↗

Series Bosch Carbon Formation Reactor Trade Study and Down-Select

Human exploration missions to Mars and other destinations beyond low Earth orbit require highly robust, reliable, and maintainable life support systems that maximize the recovery of oxygen (O2). One process in development and under consideration is the Series-Bosch System (S-Bosch). This system is a two stage reactor process that reduces carbon dioxide (CO2) with hydrogen (H2) to produce water and solid carbon. Theoretically, the Bosch process can recover 100% of the O2 from CO2 in the form of water, making it an attractive option for long duration missions. The Series Bosch system includes a reverse water gas shift (RWGS) reactor, a carbon formation reactor (CFR), a H2 extraction membrane, and a CO2extraction membrane. To further the development of oxygen recovery systems, NASA awarded Phase I contracts to several entities under the Game Changing Development Program addressing Spacecraft Oxygen Recovery (SCOR). Two of the hardware deliverables were integrated into the Carbon Dioxide Reduction Test Stand (CORTS) as carbon formation reactors and performance evaluations were executed at Marshall Space Flight Center. A trade study was performed between these two reactors along with one developed at MSFC. This paper provided the necessary information for the Advanced Exploration Systems/Life Support Systems Project (AES/LSS) managers to select a CFR for further development and discusses the process of selection.

Bosch↗

Commercial Hypersonic Transportation Market Study

In support of NASA’s evaluation of its future research thrusts for its hypersonics program, NASA commissioned independent studies of the market for commercial hypersonic transportation. SAIC and BryceTech (formerly Bryce Space and Technology) were awarded one of these independent studies to assist NASA in better understanding: (1) the passenger demand for high-speed aviation travel; (2) the pressures on the business case for developing and operating a hypersonic aircraft for the commercial aviation market; and (3) the non-technical (i.e., societal) barriers and challenges, including the steps NASA and the Government could take to overcome those barriers and challenges. Our approach to addressing these three tasks for NASA included modeling future demand and future business operations, considering global air transportation at speeds of Mach 2 to Mach 7. The team forecast premium air travel demand through 2060 and assessed the willingness of passengers of different income and wealth levels to pay to save time on flights between 800 city pairs. With the total addressable market defined, we examined industry-level business case viability for several aircraft speed and range cases. Considering operating and manufacturing costs for routes that could be serviced profitably, as well as typical profitability targets for the aviation industry, we quantified the level of RDT&E funding available to support each business case.

hypersonic aircraft↗

Independent Market Study: Commercial Hypersonic Transportation

In support of NASA’s evaluation of its future research thrusts for its hypersonics program, NASA commissioned independent studies of the market for commercial hypersonic transportation. SAIC and BryceTech (formerly Bryce Space and Technology) were awarded one of these independent studies to assist NASA in better understanding: (1) the passenger demand for high-speed aviation travel; (2) the pressures on the business case for developing and operating a hypersonic aircraft for the commercial aviation market; and (3) the non-technical (i.e., societal) barriers and challenges, including the steps NASA and the Government could take to overcome those barriers and challenges. Our approach to addressing these three tasks for NASA included modeling future demand and future business operations, considering global air transportation at speeds of Mach 2 to Mach 7. The team forecast premium air travel demand through 2060 and assessed the willingness of passengers of different income and wealth levels to pay to save time on flights between 800 city pairs. With the total addressable market defined, we examined industry-level business case viability for several aircraft speed and range cases. Considering operating and manufacturing costs for routes that could be serviced profitably, as well as typical profitability targets for the aviation industry, we quantified the level of RDT&E funding available to support each business case.

hypersonic aircraft↗

Viability of Small Dimension Crew Quarters for Surface Habitation

During early planning for the Artemis program’s sustained phase of lunar activity, NASA planners have been held to work towards a NASA reference lunar lander concept. With this activity taking place prior to the awarding of a lander contract, NASA planners cannot assume which of several potential landers will be available. This has limited habitation team engineers to a 12-metric ton mass limit for the reference concept of the lunar Surface Habitat. Consequently, minimal approaches have been applied to many habitat systems and it is important to determine acceptable volume for crew quarters. A number of both NASA and non-NASA surface habitat concepts have proposed relatively small crew quarters due to this constraint. Consequently, there is a need to collect objective test data to confirm or refute the validity of small crew quarters. NASA-STD-3001 is looked to for guidance in its many standards but offers little to no help. While prior versions called for private habitation, the current version – Revision B – calls for “individual privacy” to “accommodate social retreat.” Proposed Revision C modifications change the language slightly to “accommodate sleep and social retreat.” This is not enough guidance to determine the size of a crew quarters or even its capabilities. Unfortunately, only a small number of US spacecraft have included crew quarters, primarily the International Space Station and the Skylab Space Station. The space shuttle orbiter sometimes flew a set of private bunks that some might consider a crew quarters. All of these are dramatically smaller than US standards for minimum jail cells. The first opportunity for NASA to test a small crew quarters in a surface habitat application has been created through the Exploration Atmospheres test series, which is evaluating human performance under reduced cabin pressures. The test is converting the 20-Foot Vacuum Chamber at Johnson Space Center into a habitat, with the lower level outfitted as an EVA test area and the upper two levels for human habitation. The test will place eight people (six test subjects and two technicians) inside the chamber for eleven days. All eight will sleep in private quarters during the test. Volume limitations in the chamber forced extremely small crew quarters, measuring approximately 2 meters in length, 0.85 meters in height, and 0.9 meters in width. The test cabin pressure of 8.2 psi and elevated oxygen also introduces significant material limitations, limiting outfitting options. Nonetheless, the crew quarters design requirements were to accommodate visual separation, auditory separation, olfactory separation, tactile separation, air flow control, lighting control, single person personal computing, physical work surface access, sleep accommodations, non-sleep rest/relaxation, meditation, stretching, two-person meetings, snacking, changing clothes, viewing appearance, video communication, and audio communication. This paper will detail the acceptability of the crew quarters as measured in the October 2021 Exploration Atmosphere test. Based on this data, the viability of the type of crew quarters used in the 20 Foot Chamber will be assessed. Design recommendations for a 30-60-day Surface Habitat crew quarters will be provided, along with recommendations for future testing.

Crew Quarters↗

AiRanger™ UAS NASA SIO Program Final Report

National Aeronautics and Space Administration (NASA) awarded a Cooperative Agreement to American Aerospace, and two other companies, under the Unmanned Aircraft Systems Integration and Operationalization (SIO) demonstration with the goal of accelerating routine unmanned aircraft systems (UAS) operations in the national airspace (NAS). The team lead by American Aerospace Technologies Inc (AATI) demonstrated its UAS AiRanger™ successfully on February 25 as part of NASA’s SIO program.

Flight Demonstration↗

Framework for NASA Space Relay Satellite Services over the Next Decade: Space Relay Partnership and Services Study

The United States National Space Policy provides guidance and directives for U.S. Government agencies to purchase commercial space services and capabilities to the maximum extent practical. In alignment with this guidance, the anticipated initial NASA near-Earth space relay operating capability over the next decade will be comprised of the existing NASA-owned Space Network infrastructure supplemented with commercially-owned and -provided relay satellites and their associated ground stations. NASA awarded contracts to eight study vendors in May 2019 to investigate the feasibility of applying commercial services concepts to the NASA user mission community. The studies, managed by NASA Glenn Research Center, detailed ten study topic areas. NASA completed the analysis and synthesis of these eight industry studies and other relevant information. The responses from the study vendors indicated a strong interest in providing commercial services to NASA missions, and included a wide range of proposed architectures and demonstration ideas. Overall, the responses indicated that a mature and healthy competitive environment exists across a broad spectrum of industry to deliver high-rate space relay services in the next three to five years. This paper will present an overview of the study, analysis and synthesis processes along with common themes and conclusions, and provide next steps and recommendations. The results of this study serve as a foundation for ongoing efforts to acquire interoperable space relay services for NASA missions from multiple commercial service providers.

Eric Knoblock↗

Framework for NASA Space Relay Satellite Services over the Next Decade: Space Relay Partnership and Services Study

The United States National Space Policy provides guidance and directives for U.S. Government agencies to purchase commercial space services and capabilities to the maximum extent practical. In alignment with this guidance, the anticipated initial NASA near-Earth space relay operating capability over the next decade will be comprised of the existing NASA-owned Space Network infrastructure supplemented with commercially-owned and -provided relay satellites and their associated ground stations. NASA awarded contracts to eight study vendors in May 2019 to investigate the feasibility of applying commercial services concepts to the NASA user mission community. The studies, managed by NASA Glenn Research Center, detailed ten study topic areas. NASA completed the analysis and synthesis of these eight industry studies and other relevant information. The responses from the study vendors indicated a strong interest in providing commercial services to NASA missions, and included a wide range of proposed architectures and demonstration ideas. Overall, the responses indicated that a mature and healthy competitive environment exists across a broad spectrum of industry to deliver high-rate space relay services in the next three to five years. This paper will present an overview of the study, analysis and synthesis processes along with common themes and conclusions, and provide next steps and recommendations. The results of this study serve as a foundation for ongoing efforts to acquire interoperable space relay services for NASA missions from multiple commercial service providers.

Eric Knoblock↗

The Light Bender Concept for Power Distribution on a Lunar Base

Power generation and distribution is a challenge for a future lunar base. A promising concept for lunar power distribution is the light bender—a system that utilizes autonomously deployed mirrors to reflect sunlight to shaded regions on the lunar surface. The light bender system consists of two components: tandem reflector mirrors (TRM) which reflect sunlight to a permanently shaded region and the Cassegrain Fresnel distribution optics (CFDO) which collects the reflected light from the TRM and redirects light to local consumer nodes. This paper describes the light bender system concepts and the associated system analysis and technology development performed as part of a 2021 NASA Innovative Advanced Concepts (NIAC) phase I award.

Bradford Robertson↗

The Light Bender Concept for Power Distribution on a Lunar Base

Power generation and distribution is a challenge for a future lunar base. A promising concept for lunar power distribution is the light bender—a system that utilizes autonomously deployed mirrors to reflect sunlight to shaded regions on the lunar surface. The light bender system consists of two components: tandem reflector mirrors (TRM) which reflect sunlight to a permanently shaded region and the Cassegrain Fresnel distribution optics (CFDO) which collects the reflected light from the TRM and redirects light to local consumer nodes. This paper describes the light bender system concepts and the associated system analysis and technology development performed as part of a 2021 NASA Innovative Advanced Concepts (NIAC) phase I award.

Bradford Robertson↗

A Gene Expression and Histologic Approach to Study Production and Outflow of Cerebrospinal Fluid in Hindlimb Suspended Rats

INTRODUCTION: The Spaceflight Associated Neuro-ocular Syndrome (SANS) is thought to be associated with weightlessness-induced cephalad fluid shift, possibly associated with a chronic elevation of intracranial pressure (ICP) during long-duration ISS missions. Changes in cerebrospinal fluid (CSF) dynamics and cranial compliance might be involved in the ICP increase. It is not known whether CSF production and/or outflow are altered in microgravity, but changes at the molecular and cellular level in the structures that produce and regulate the transcellular and paracellular secretion and reabsorption of CSF may be relevant. In this study, we used the rat hindlimb suspension (HS) model to examine the relationship between intracranial pressure (ICP) and the cellular responses to the prolonged change in body posture elicited specifically in the choroid plexus (CP). This was evaluated by transcriptomics, histopathology and ultrastructure of the CP and arachnoid villi (AV). METHODS: ICP was measured by telemetry. The morphology, ultrastructure, and gene expression profile of the CP was examined using male 9-month-old Long Evans rats subjected to HS for 14 and 90 days. A subset of animals completing 90-day HS returned to normal posture for 14 and 90 additional days of recovery. All HS rats had age-matched cage controls maintained in normal posture. A group of animals was maintained in conditions of 1% CO2 throughout the entire protocol. The rat brains were carefully removed and preserved for various analyses, including transmission electron microscopy (TEM), immunohistochemical analysis of specific targets involved in CSF regulation, and RNA sequencing analysis of laser capture micro-dissected CP tissue from the lateral ventricles. SUMMARY OF RESULTS: The ICP record was limited, which impacted any conclusions derived from the results. However, the ICP of rats in the CO2-enriched atmosphere appeared increased compared to those in a normal air composition. HS of rats resulted in an altered transcriptomics profile in the choroid plexus compared to animals maintained at normal posture. This was observed during both the HS period and during normal posture recovery period following 90 days of suspension. The greatest number of differentially expressed genes was observed at 90 days HS. Elevated CO2 also led to a different transcriptomics profile in the CP. The histology and ultrastructure results should be considered preliminary due to the limited number of samples. Examination of the CP by TEM showed blood vessel congestion and microvilli swelling in the CP, as well as some subpial and periventricular gliosis, but no associations were observed with treatment. There was no evidence of a reduced clearance of b-amyloid in the periventricular, perivascular and subpial regions of the brain in HS animals versus those in normal posture. Immunohistochemical staining of aquaporin 4, showed the localization to the subpial region and ependyma with increased immunoreactivity in the brains of CO2 exposed rats. This work was supported by awards NNX15AW48G to S.Z. Animal tissue and live data was provided under a tissue/data sharing agreement with Dr. Charles Fuller, UC Davis.

S B Zanello↗

Artemis Deep Space Habitation: Enabling a Sustained Human Presence on the Moon and Beyond

As NASA and its partners’ capabilities for human exploration of deep space continue to mature, so too does its roadmap toward a sustained crewed presence on the surface of the Moon and eventual human missions to Mars. The first launch of the Space Launch System and Orion crew vehicle, the contract award for the first demonstrations of a Human Landing System, and the beginning of construction on the initial elements of the lunar Gateway have marked major milestones toward NASA’s near-term exploration goals: a long-duration outpost in orbit around the Moon and the next footsteps on the lunar surface. At the same time, NASA is in the early phases of planning the capabilities that will be needed for long-term exploration. Among the common elements that will be required by long-duration stays on the lunar surface, transit to Mars, and Martian surface expeditions will be new habitats unlike any flown to date. NASA is currently working on development of both architectures for those habitats and on the technological advancements that will enable them, with an eye toward systems that will not only extend mission operations but also provide for living quarters that will keep the crew happy and healthy throughout their expeditions. Beyond the Gateway habitation needs, these capabilities will need to be defined and advanced to support the initial lunar surface missions and to prepare for human missions to the Mars system. The Surface Habitat is the current concept in consideration to serve as this initial surface habitat that will extend the crew mission durations. It will provide 30-to-60-day habitability for a crew of up to four allowing for the astronauts to explore farther and longer on each visit to the lunar surface. NASA is also currently reviewing opportunities to use current or near-term in-space habitation systems as proving grounds or precursors for keeping astronauts safe and healthy during future transits to Mars. Already, the International Space Station (ISS) is being used for implementation of next-generation life-support systems that will inform those used in exploration habitats, and the operations approach for ISS is providing lessons-learned for future science operations around or on the Moon.1U.S. Government work not protected by U.S. copyright While a suite of habitation concepts is currently under study within NASA, the agency is also working closely with U.S. industry through the Next Space Technologies for Exploration Partnerships (NextSTEP) activity to understand their concepts for commercially provided habitation capabilities as well as close coordination with international partners to understand their desires for in-space and surface habitation. This paper will provide a status of these concepts and partnership activities as well as potential future technology and architecture development paths.

Habitat↗

A Gene Expression and Histologic Approach to Study Production and Outflow of Cerebrospinal Fluid in Hindlimb Suspended RATS

INTRODUCTION: The Spaceflight Associated Neuro-ocular Syndrome (SANS) is thought to be associated with weightlessness-induced cephalad fluid shift, possibly associated with a chronic elevation of intracranial pressure (ICP) during long-duration ISS missions. Changes in cerebrospinal fluid (CSF) dynamics and cranial compliance might be involved in the ICP increase. It is not known whether CSF production and/or outflow are altered in microgravity, but changes at the molecular and cellular level in the structures that produce and regulate the transcellular and paracellular secretion and reabsorption of CSF may be relevant. In this study, we used the rat hindlimb suspension (HS) model to examine the relationship between intracranial pressure (ICP) and the cellular responses to the prolonged change in body posture elicited specifically in the choroid plexus (CP). This was evaluated by transcriptomics, histopathology and ultrastructure of the CP and arachnoid villi (AV). METHODS: ICP was measured by telemetry. The morphology, ultrastructure, and gene expression profile of the CP was examined using male 9-month-old Long Evans rats subjected to HS for 14 and 90 days. A subset of animals completing 90-day HS returned to normal posture for 14 and 90 additional days of recovery. All HS rats had age-matched cage controls maintained in normal posture. A group of animals was maintained in conditions of 1% CO2 throughout the entire protocol. The rat brains were carefully removed and preserved for various analyses, including transmission electron microscopy (TEM), immunohistochemical analysis of specific targets involved in CSF regulation, and RNA sequencing analysis of laser capture micro-dissected CP tissue from the lateral ventricles. SUMMARY OF RESULTS: The ICP record was limited, which impacted any conclusions derived from the results. However, the ICP of rats in the CO2-enriched atmosphere appeared increased compared to those in a normal air composition. HS of rats resulted in an altered transcriptomics profile in the choroid plexus compared to animals maintained at normal posture. This was observed during both the HS period and during normal posture recovery period following 90 days of suspension. The greatest number of differentially expressed genes was observed at 90 days HS. Elevated CO2 also led to a different transcriptomics profile in the CP. The histology and ultrastructure results should be considered preliminary due to the limited number of samples. Examination of the CP by TEM showed blood vessel congestion and microvilli swelling in the CP, as well as some subpial and periventricular gliosis, but no associations were observed with treatment. There was no evidence of a reduced clearance of b-amyloid in the periventricular, perivascular and subpial regions of the brain in HS animals versus those in normal posture. Immunohistochemical staining of aquaporin 4, showed the localization to the subpial region and ependyma with increased immunoreactivity in the brains of CO2 exposed rats. This work was supported by awards NNX15AW48G to S.Z. Animal tissue and live data was provided under a tissue/data sharing agreement with Dr. Charles Fuller, UC Davis.

S B Zanello↗