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At least 1,693 records · Page 94

Isolation of Methane Enriched Bacterial Communities and Application as Wheat Biofertilizer under Drought Conditions: An Environmental Contribution

The search for methanotrophs as plant-growth-promoting rhizobacteria (PGPR) presents an important contribution to mitigating the impact of global warming by restoring the natural soil potential for consuming methane while benefiting plants during droughts. Our in silico simulations suggest that water, produced as a byproduct of methane oxidation, can satisfy the cell growth requirement. In addition to water, methanotrophs can produce metabolites that stimulate plant growth. Considering this, we proposed that applying methanotrophs as PGPR can alleviate the effect of droughts on crops, while stimulating atmospheric methane consumption. In this work, we isolated a series of methanotrophic communities from the rhizospheres of different crops, including Italian sweet pepper and zucchini, using an atmosphere enriched with pure methane gas, to determine their potential for alleviating drought stress in wheat plants. Subsequently, 23 strains of nonmethanotrophic bacteria present in the methanotrophic communities were isolated and characterized. We then analyzed the contribution of the methane-consuming consortia to the improvement of plant growth under drought conditions, showing that some communities contributed to increases in the wheat plants’ lengths and weights, with statistically significant differences according to ANOVA models. Furthermore, we found that the presence of methane gas can further stimulate the plant–microbe interactions, resulting in larger plants and higher drought tolerance.

59 BASIC BIOLOGICAL SCIENCES↗

Bacterial Volatile Organic Compounds as a Strategy to Increase Drought Tolerance in Maize (Zea mays L.): Influence on Plant Biochemistry

Maize is highly susceptible to drought, which affects growth and yield. This study investigated how bacterial volatile organic compounds (BVOCs) affect maize drought tolerance. Drought reduced shoot size but increased root length, an adaptation for accessing deeper soil moisture. BVOCs from strain D12 significantly increased root length and shoot growth under drought conditions. Drought also altered root biochemistry, decreasing enzyme activity, and increased osmolyte levels. BVOCs from strains F11 and FS4-14 further increased osmolyte levels but did not protect membranes from oxidative damage, while BVOCs from strains D12 and D7 strains reduced osmolyte levels and cell damage. In shoots, drought increased the levels of osmolytes and oxidative stress markers. BVOCs from FS4-14 had minimal effects on shoot biochemistry. BVOCs from D12 and F11 partially restored metabolic activity but did not reduce cell damage. BVOCs from D7 reduced metabolic activity and cell damage. These results suggest that BVOCs can modulate the biochemical response of maize to drought, with some strains evidencing the potential to enhance drought tolerance.

Lopes, Tiago (ORCID:0000000330603315)↗

Advances in Electrospun Poly(ε-caprolactone)-Based Nanofibrous Scaffolds for Tissue Engineering

Tissue engineering has great potential for the restoration of damaged tissue due to injury or disease. During tissue development, scaffolds provide structural support for cell growth. To grow healthy tissue, the principal components of such scaffolds must be biocompatible and nontoxic. Poly(ε-caprolactone) (PCL) is a biopolymer that has been used as a key component of composite scaffolds for tissue engineering applications due to its mechanical strength and biodegradability. However, PCL alone can have low cell adherence and wettability. Blends of biomaterials can be incorporated to achieve synergistic scaffold properties for tissue engineering. Electrospun PCL-based scaffolds consist of single or blended-composition nanofibers and nanofibers with multi-layered internal architectures (i.e., core-shell nanofibers or multi-layered nanofibers). Nanofiber diameter, composition, and mechanical properties, biocompatibility, and drug-loading capacity are among the tunable properties of electrospun PCL-based scaffolds. Scaffold properties including wettability, mechanical strength, and biocompatibility have been further enhanced with scaffold layering, surface modification, and coating techniques. In this article, we review nanofibrous electrospun PCL-based scaffold fabrication and the applications of PCL-based scaffolds in tissue engineering as reported in the recent literature.

Polymer Science↗

Mitigation of Cu-Induced Grain Boundary Sensitization in Steel Wire Rods Through a Desensitization Heat Treatment

Steel wire rods are essential for manufacturing high-strength steel tire cords. Yet, the presence of residual copper (Cu) in recycled steel can cause grain-boundary sensitization, embrittlement, and deterioration of the mechanical performance of the final product. This study introduces a desensitization heat treatment step designed to redistribute Cu away from austenite grain boundaries after sensitization occurs. The treatment consists of a 10 min dwell at 1000 °C in a 5%H 2 -Ar reducing atmosphere followed by quench. The temperature and hold time were selected based on diffusion calculations to promote solid-state back diffusion of Cu without altering grain morphology. Experimental validation showed that the dwell step reduced the length of Cu-rich sensitized zones of steel wire rod samples containing 0.21 wt.% Cu by approximately 89% and restored the mechanical properties to nearly 95–98% relative to low-Cu baseline steel (0.01 wt.% Cu). Compared with sensitized and as-obtained samples, these results highlight the effectiveness of the proposed method in improving both the microstructure and tensile performance of recycled steel wire rods, enabling their potential application in tire manufacturing.

copper↗

Quantification of Off-Channel Inundated Habitat for Pacific Chinook Salmon ( Oncorhynchus tshawytscha ) along the Sacramento River, California, Using Remote Sensing Imagery

Off-channel areas are one of the most impacted aquatic habitats by humans globally, as extensive agricultural and urban development has limited them to roughly 10% of historical extent. This is also true for California’s Sacramento River Valley, where historically frequent widespread inundation has been reduced to a few off-channel water bodies along the mid-Sacramento River. This remaining shallow-water habitat provides crucial ecological benefits to multiple avian and fish species, but especially to floodplain-adapted species such as Chinook salmon (Oncorhynchus tshawytscha). Characterizing spatiotemporal off-channel dynamics, including inundation extent and residence time, is fundamental to better understanding the intrinsic value of such habitats and their potential to support recovery actions. Remote sensing techniques have been increasingly used to map surface water at regional and local scales, with improved resolutions. As such, this study maps off-channel inundation areas and describes their temporal dynamics by analyzing pixel-based time- series of multiple water indices, modified Normalized Difference Water Index (mNDWI) and the Automated Water Extraction Index (AWEI), generated from LandSat-8 and Sentinel-2 data between 2013–2021. Quantified off-channel area was similar with each water index and method used, but improved performance was associated with Sentinel-2 products and AWEI index to identify wetted areas under lower mainstem discharges. Results indicate an uneven distribution of off-channel habitat in the study area, with limited inundated areas in upstream reaches (<16% of total off-channel area for greater flows). In addition, much less habitat exists for flows under 400 m 3 /s, an important migration cue for endangered winter-run Chinook salmon, limiting juvenile access to areas with enhanced rearing conditions. Off-channel habitat residence times averaged between 7 and 16 days, primarily defined by the rate of receding flows, with rapid flow recession providing marginal off-channel habitat. This study shows reasonable performance of moderate resolution LandSat-8 and Sentinel-2 remote sensing imagery to characterize shallow-water inundated habitat in higher-order rivers, and as a method to inform restoration and native fish recovery efforts.

54 ENVIRONMENTAL SCIENCES↗

Bird Species Use of Bioenergy Croplands in Illinois, USA—Can Advanced Switchgrass Cultivars Provide Suitable Habitats for Breeding Grassland Birds?

Grassland birds have sustained significant population declines in the United States through habitat loss, and replacing lost grasslands with bioenergy production areas could benefit these species and the ecological services they provide. Point count surveys and autonomous acoustic monitoring were used at two field sites in Illinois, USA, to determine if an advanced switchgrass cultivar that is being used for bioenergy feedstock production could provide suitable habitats for grassland and other bird species. At the Brighton site, the bird use of switchgrass plots was compared to that of corn plots during the breeding seasons of 2020–2022. At the Urbana site, the bird use of restored prairie, switchgrass, and Miscanthus × giganteus was studied in the 2022 breeding season. At Brighton, Common Yellowthroat, Dickcissel, Grasshopper Sparrow, and Sedge Wren occurred on switchgrass plots more often than on corn; Common Yellowthroat and Dickcissel increased on experimental plots as the perennial switchgrass increased in height and density over the study period; and the other two species declined over the same period. At Urbana, Dickcissel was most frequent in prairie and switchgrass; Common Yellowthroat was most frequent in miscanthus and switchgrass. These findings suggest that advanced switchgrass cultivars could provide suitable habitats for grassland birds, replace lost habitats, and contribute to the recovery of these vulnerable species.

59 BASIC BIOLOGICAL SCIENCES↗

The Susceptibility of Juvenile American Shad to Rapid Decompression and Fluid Shear Exposure Associated with Simulated Hydroturbine Passage

Throughout many areas of their native range, American shad (Alosa sapidissima) and other Alosine populations are in decline. Though several conditions have influenced these declines, hydropower facilities have had significant negative effects on American shad populations. Hydropower facilities expose ocean-migrating American shad to physical stressors during passage through hydropower facilities, including strike, rapid decompression, and fluid shear. In this laboratory-based study, juvenile American shad were exposed separately to rapid decompression and fluid shear to determine their susceptibility to these stressors and develop dose–response models. These dose–response relationships can help guide the development and/or operation of hydropower turbines and facilities to reduce the negative effects to American shad. Relative to other species, juvenile American shad have a high susceptibility to both rapid decompression and fluid shear. Reducing or preventing exposure to these stressors at hydropower facilities may be a potential method to assist in the effort to restore American shad populations.

13 HYDRO ENERGY↗

The Straightening of a River Meander Leads to Extensive Losses in Flow Complexity and Ecosystem Services

To assist river restoration efforts we need to slow the rate of river degradation. This study provides a detailed explanation of the hydraulic complexity loss when a meandering river is straightened in order to motivate the protection of river channel curvature. We used computational fluid dynamics (CFD) modeling to document the difference in flow dynamics in nine simulations with channel curvature (C) degrading from a well-established tight meander bend (C = 0.77) to a straight channel without curvature (C = 0). To control for covariates and slow the rate of loss to hydraulic complexity, each of the nine-channel realizations had equivalent bedform topography. The analyzed hydraulic variables included the flow surface elevation, streamwise and transverse unit discharge, flow velocity at streamwise, transverse, and vertical directions, bed shear stress, stream function, and the vertical hyporheic flux rates at the channel bed. The loss of hydraulic complexity occurred gradually when initially straightening the channel from C = 0.77 to C = 0.33 (i.e., the radius of the channel is three-times the channel width), and additional straightening incurred rapid losses to hydraulic complexity. Other studies have shown hydraulic complexity provides important riverine habitat and is positively correlated with biodiversity. This study demonstrates how hydraulic complexity can be gradually and then rapidly lost when unwinding a river, and hopefully will serve as a cautionary tale.

54 ENVIRONMENTAL SCIENCES↗

Quantifying Drivers of Methane Hydrobiogeochemistry in a Tidal River Floodplain System

The influence of coastal ecosystems on global greenhouse gas (GHG) budgets and their response to increasing inundation and salinization remains poorly constrained. In this study, we have integrated an uncertainty quantification (UQ) and ensemble machine learning (ML) framework to identify and rank the most influential processes, properties, and conditions controlling methane behavior in a freshwater floodplain responding to recently restored seawater inundation. Our unique multivariate, multiyear, and multi-site dataset comprises tidal creek and floodplain porewater observations encompassing water level, salinity, pH, temperature, dissolved oxygen (DO), dissolved organic carbon (DOC), total dissolved nitrogen (TDN), partial pressure of carbon dioxide (pCO 2 ), nitrous oxide (pN 2 O), methane (pCH 4 ), and the stable isotopic composition of methane (δ 13 CH 4 ). Additionally, we incorporated topographical data, soil porosity, hydraulic conductivity, and water retention parameters for UQ analysis using a previously developed 3D variably saturated flow and transport floodplain model for a physical mechanistic understanding of factors influencing groundwater levels and salinity and, therefore, CH 4 . Principal component analysis revealed that groundwater level and salinity are the most significant predictors of overall biogeochemical variability. The ensemble ML models and UQ analyses identified DO, water level, salinity, and temperature as the most influential factors for porewater methane levels and indicated that approximately 80% of the total variability in hourly water levels and around 60% of the total variability in hourly salinity can be explained by permeability, creek water level, and two van Genuchten water retention function parameters: the air-entry suction parameter α and the pore size distribution parameter m. These findings provide insights on the physicochemical factors in methane behavior in coastal ecosystems and their representation in local- to global-scale Earth system models.

54 ENVIRONMENTAL SCIENCES↗

Digital Twin Empowered PV Power Prediction

The accurate prediction of photovoltaic (PV) power generation is significant to ensure the economic and safe operation of power systems. To this end, the paper proposes a new digital twin (DT) empowered PV power prediction framework that is capable of ensuring reliable data transmission and employing the DT to achieve high accuracy of power prediction. With this framework, considering potential data contamination in the collected PV data, a generative adversarial network is employed to restore the historical data set, which offers a prerequisite to ensure accurate mapping from the physical space to the digital space. Further, a new DT empowered PV power prediction method is proposed. Therein, we model a DT that encompasses a digital physical model for reflecting the physical operation mechanism and a neural network model (i.e., a parallel network of convolution and bidirectional long-short-term memory model) for capturing the hidden spatial-temporal features. The proposed method enables the use of the DT to take advantages of the digital physical model and the neural network model, resulting in enhanced prediction accuracy. Finally, a real data set is conducted to access the effectiveness of the proposed method.

14 SOLAR ENERGY↗

Regional Power System Black Start with Run-of-river Hydropower Plant and Battery Energy Storage

Battery energy storage systems (BESSs) are an important asset for power systems with high integration levels of renewable energy, and they can be controlled to provide various critical services to the power grid. This paper presents the real-world experience of using a megawatt-scale BESS with grid-following (GFL) and grid-forming (GFM) controls and a run-of-river (ROR) hydropower plant to restore a regional power system. To demonstrate this, we carry out power-hardware-in-the-loop experiments integrating an actual GFL- or GFM-controlled BESS and a load bank. Both the simulation and experimental results presented in this paper show the different roles of GFL- or GFM-controlled BESS in power system black starts. The results provide further insight for system operators on how GFL- or GFM-controlled BESS can enhance grid stability and how an ROR hydropower plant can be converted into a black-start-capable unit with the support of a small-capacity BESS. The results show that an ROR hydropower plant combined with a BESS has the potential of becoming one of enabling elements to perform bottom-up black-start schemes as opposed to conventional bottom-down method, thus enhancing the system resiliency and robustness.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Schottky contacts on ultra-high-pressure-annealed GaN with high rectification ratio and near-unity ideality factor

Here we investigate the electrical characteristics of Ni Schottky contacts on n-type GaN films that have undergone ultra-high-pressure annealing (UHPA), a key processing step for activating implanted Mg. Contacts deposited on these films exhibit low rectification and high leakage current compared to contacts on as-grown films. By employing an optimized surface treatment to restore the GaN surface following UHPA, we obtain Schottky contacts with a high rectification ratio of ~10 9 , a near-unity ideality factor of 1.03, and a barrier height of ~0.9 eV. These characteristics enable the development of GaN junction barrier Schottky diodes employing Mg implantation and UHPA.

42 ENGINEERING↗

Durability of Disposable N95 Mask Material When Exposed to Improvised Ozone Gas Disinfection

The principle finding of this report is that both commercial and a novel material used for N95 mask filters can endure many cycles of disinfection by ozone gas (20 ppm for 30 minutes) without detectable degradation or loss of filtration efficiency. N95 masks and surgical masks (hereafter referred to as masks) typically use a filtration material fabricated from meltblown polypropylene. To achieve maximum filtration efficiency while maintaining a reasonable pressure drop, these nonwoven fabrics are also electrostatically charged (corona discharge is the most common method used), to maximize attraction and capture of aerosols and solid particulates. Under normal circumstances, the reuse of masks is generally discouraged, but in times of crisis has become a necessity, making disinfection after each use a necessity. To be acceptable, any disinfection procedure must cause minimal degradation to the performance of the filter material. Possible performance degradation mechanisms include mechanical damage, loss of electrostatic charge, or both. One of the most practical and direct ways to measure combined mechanical and electrostatic integrity, and the subsequent ability to reuse mask filter material, is by the direct measurement of filtration efficiency. In this paper, we report that small numbers of disinfection cycles at reasonable virucidal doses of ozone do not significantly degrade the filtration efficiency of meltblown polypropylene filter material. By comparison, laundering quickly results in a significant loss of filtration efficiency and requires subsequent recharging to restore the electrostatic charge and filtration efficiency. A common assumption among biomedical scientists that ozone is far too destructive for this application. However, these direct measurements show that mask materials, specifically the filtration material, can withstand dozens of ozone disinfection cycles without any measurable degradation of filtration efficiency, nor any visible discoloration or loss of fiber integrity. The data are clear: when subjected to a virucidal dose of ozone for a much longer duration than is required for viral inactivation, there was no degradation of N95 filtration efficiency. The specific dosages of ozone needed for ~99% viral inactivation are thought to be at least 10 ppm for up to 30 minutes based upon an extensive literature review, but to standardize our testing, we consider a dose of 20 ppm for 30 minutes to be a reasonable and conservatively high ozone disinfection cycle. Finally, the material tested in this study withstood dosages of up to 200 ppm for 90 minutes, or alternatively 20 ppm for up to 36 hours, without detectable degradation, and further testing suggests that up to 30 or more disinfection cycles (at 20 ppm for 30 minutes) would result in less than a 5% loss of filtration efficiency. This report does not address the effect of ozone cycling on other mask components, such as elastics.

60 APPLIED LIFE SCIENCES↗

Refined Telluric Absorption Correction for Low-resolution Ground-based Spectroscopy: Resolution and Radial Velocity Effects in the O{sub 2}A-band for Exoplanets and K i Emission Lines

Telluric correction of spectroscopic observations is either performed via standard stars that are observed close in time and airmass along with the science target, or recently growing in importance, by theoretical telluric absorption modeling. Both approaches work fine when the telluric lines are resolved, i.e., at a spectral resolving power larger than about 10,000, and it is sufficient to facilitate the detection of spectral features at lower resolution. However, a meaningful quantitative analysis also requires the reliable recovery of line strengths. Here, we show for the Fraunhofer A-band of molecular O{sub 2} that the standard telluric correction approach fails in this at lower spectral resolutions, as an example for the general problem. Doppler-shift-dependent errors of the restored flux may arise, which can amount to more than 50% in extreme cases, depending on the line shapes of the target spectral features. Two applications are discussed: the recovery of the O{sub 2} band in the reflected light of an Earth analog atmosphere, as facilitated potentially in the future using an orbiting starshade and a ground-based extremely large telescope; and the recovery of the intrinsic ratio of the K i lines in the post-nova V4332 Sgr tracing the optical depth of the emitting region, to exemplify the relevance using present-day instrumentation. We show how one should derive correction functions for the compensation of the error in dependence of radial velocity shift, spectral resolution, and target line-profile function by use of high-resolution atmospheric transmission modeling, which has to be solved for the individual case.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Inpainting Galactic Foreground Intensity and Polarization Maps Using Convolutional Neural Networks

The Deep Convolutional Neural Networks (DCNNs) have been a popular tool for image generation and restoration. In this work, we applied DCNNs to the problem of inpainting non-Gaussian astrophysical signal, in the context of Galactic diffuse emissions at the millimetric and submillimetric regimes, specifically Synchrotron and Thermal Dust emissions. Both signals are affected by contamination at small angular scales due to extragalactic radio sources (the former) and dusty star-forming galaxies (the latter). Furthermore, we compare the performance of the standard diffusive inpainting with that of two novel methodologies relying on DCNNs, namely Generative Adversarial Networks and Deep-Prior. We show that the methods based on the DCNNs are able to reproduce the statistical properties of the ground-truth signal more consistently with a higher confidence level.

79 ASTRONOMY AND ASTROPHYSICS↗

The Role of Strong Magnetic Fields in Stabilizing Highly Luminous Thin Disks

Abstract We present and analyze a set of three-dimensional, global, general relativistic radiation magnetohydrodynamic simulations of thin, radiation-pressure-dominated accretion disks surrounding a nonrotating, stellar-mass black hole. The simulations are initialized using the Shakura–Sunyaev model with a mass accretion rate of M ̇ = 3 L Edd / c 2 (corresponding to L = 0.17 L Edd ). Our previous work demonstrated that such disks are thermally unstable when accretion is driven by an α -viscosity. In the present work, we test the hypothesis that strong magnetic fields can both drive accretion through magnetorotational instability and restore stability to such disks. We test four initial magnetic field configurations: (1) a zero-net-flux case with a single, radially extended set of magnetic field loops (dipole), (2) a zero-net-flux case with two radially extended sets of magnetic field loops of opposite polarity stacked vertically (quadrupole), (3) a zero-net-flux case with multiple radially concentric rings of alternating polarity (multiloop), and (4) a net-flux, vertical magnetic field configuration (vertical). In all cases, the fields are initially weak, with a gas-to-magnetic pressure ratio ≳100. Based on the results of these simulations, we find that the dipole and multiloop configurations remain thermally unstable like their α -viscosity counterpart, in our case collapsing vertically on the local thermal timescale and never fully recovering. The vertical case, on the other hand, stabilizes and remains so for the duration of our tests (many thermal timescales). The quadrupole case is intermediate, showing signs of both stability and instability. The key stabilizing factor is the ability of specific field configurations to build up and sustain strong, P mag ≳ 0.5 P tot , toroidal fields near the midplane of the disk. We discuss the reasons why certain configurations are able to do this effectively and others are not. We then compare our stable simulations to the standard Shakura–Sunyaev disk.

79 ASTRONOMY AND ASTROPHYSICS↗

Suppression of Composition g -modes in Chemically Equilibrating Warm Neutron Stars

We investigate the impact of chemical equilibration and the resulting bulk viscosity on nonradial oscillation modes of warm neutron stars at temperatures up to T ≈ 5 MeV, relevant for protoneutron stars and neutron star postmerger remnants. In this regime, the relaxation rate of weak interactions becomes comparable to the characteristic frequencies of composition g-modes in the core, resulting in resonant damping. To capture this effect, we introduce the dynamical sound speed, a complex, frequency-dependent generalization of the adiabatic sound speed that encodes both the restoring force and the dissipative effects of bulk compression. Using realistic weak reaction rates and three representative equations of state, we compute the complex frequencies of composition g-modes with finite-temperature profiles. We find that bulk viscous damping becomes increasingly significant with temperature and can completely suppress composition g-modes. In contrast, the f-mode remains largely unaffected by bulk viscosity due to its nearly divergence-free character. Our results highlight the sensitivity of g-mode behavior to thermal structure, weak reaction rates, and the equation of state, and establish the dynamical sound speed as a valuable descriptor characterizing oscillation properties in dissipative neutron star matter.

Neutron stars↗

Reduced Atmospheres of Post-impact Worlds: The Early Earth

Impacts may have had a significant effect on the atmospheric chemistry of the early Earth. Reduced phases in the impactor (e.g., metallic iron) can reduce the planet's H 2 O inventory to produce massive atmospheres rich in H 2 . While previous studies have focused on the interactions between the impactor and atmosphere in such scenarios, we investigate two further effects: (1) the distribution of the impactor's iron inventory during impact between the target interior, target atmosphere, and escaping the target; and (2) interactions between the post-impact atmosphere and the impact-generated melt phase. We find that these two effects can potentially counterbalance each other, with the melt–atmosphere interactions acting to restore reducing power to the atmosphere that was initially accreted by the melt phase. For a ~10 22 kg impactor, when the iron accreted by the melt phase is fully available to reduce this melt, we find an equilibrium atmosphere with H 2 column density ~10 4 moles cm –2 (pH 2 ~ 120 bars, X H2 ~ 0.77), consistent with previous estimates. However, when the iron is not available to reduce the melt (e.g., sinking out in large diameter blobs), we find significantly less H 2 (7 × 10 2 – 5 × 10 3 moles cm –2 , pH 2 ≲ 60 bars, X H2 ≲ 0.41). These lower H 2 abundances are sufficiently high that species important to prebiotic chemistry can form (e.g., NH 3 , HCN), but sufficiently low that the greenhouse heating effects associated with highly reducing atmospheres, which are problematic to such chemistry, are suppressed. The manner in which iron is accreted by the impact-generated melt phase is critical in determining the reducing power of the atmosphere and resolidified melt pool in the aftermath of impact.

79 ASTRONOMY AND ASTROPHYSICS↗