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At least 1,693 records · Page 94

Individual Differences in Human Reliability Analysis

While human reliability analysis (HRA) methods include uncertainty in quantification, the nominal model of human error in HRA typically assumes that operator performance does not vary significantly when they are given the same initiating event, indicators, procedures, and training, and that any differences in operator performance are simply aleatory (i.e., random). While this assumption generally holds true when performing routine actions, variability in operator response has been observed in multiple studies, especially in complex situations that go beyond training and procedures. As such, complexity can lead to differences in operator performance (e.g., operator understanding and decision-making). Furthermore, psychological research has shown that there are a number of known antecedents (i.e., attributable causes) that consistently contribute to observable and systematically measurable (i.e., not random) differences in behavior. This paper reviews examples of individual differences taken from operational experience and the psychological literature. The impact of these differences in human behavior and their implications for HRA are then discussed. We propose that individual differences should not be treated as aleatory, but rather as epistemic. Ultimately, by understanding the sources of individual differences, it is possible to remove some epistemic uncertainty from analyses.

99 GENERAL AND MISCELLANEOUS↗

A REVIEW OF HUMAN-SYSTEM INTERFACE DESIGN ISSUES OBSERVED DURING ANALOG-TO-DIGITAL AND DIGITAL-TO-DIGITAL MIGRATIONS IN U.S. NUCLEAR POWER PLANTS

The United States (U.S.) Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) program is developing a scientific basis through targeted research and development (R&D) to support the U.S. nuclear power plant (NPP) fleet in extending their existing licensing period and ensuring their long-term reliability, productivity, safety, and security. Over the last several years, human factors engineering (HFE) professionals at the Idaho National Laboratory (INL) have supported the LWRS Advanced Instrumentation, Information, and Control (II&C) System Technologies pathway across several U.S. commercial NPPs in analog-to-digital migrations (i.e., turbine control systems) and digital-to-digital migrations (i.e., Safety Parameter Display System). These efforts have included in-depth human factors evaluation of proposed human-system interface (HSI) design concepts against established U.S. Nuclear Regulatory Commission (NRC) design guidelines from NUREG-0700, Rev 2 to inform subsequent HSI design prior to transitioning into Verification and Validation. This paper discusses some of the overarching design issues observed from these past HFE evaluations. In addition, this work presents some observed challenges such as common tradeoffs utilities are likely to face when introducing new HSI technologies into NPP hybrid control rooms. The primary purpose of this work is to distill these observed design issues into general HSI design guidance that industry can use in early stages of HSI design.

99 GENERAL AND MISCELLANEOUS↗

Comparison of Human Performance between Operators and Students Using Rancor Microworld Simulator: A Preliminary Result

This paper describes an experiment using the Rancor Microworld simulator to probe into differences in human performance between students and actual operators. Six human performance measures – 1) time, 2) error, 3) workload, 4) situational awareness, 5) attention patterns, and 6) number of manipulations – are measured from experiments. The experiment has been conducted for 11 operators and 20 students so far. More experiments and analyses will be continued. Further experimental studies using Rancor Microworld are expected to be able to collect additional data that may be missing from full-scope studies and contribute to items that can support human reliability data.

99 GENERAL AND MISCELLANEOUS↗

Study on the assessment method of human error probabilities in the digitalized main control room - 170

Recently, nuclear power plants (NPPs) in Republic of Korea have been constructed with the new type of main control room (MCR) which is called as digitalized MCR. In digitalized MCR, as digital technologies are being adopted, the environment of MCR has been considerably changed. The operators may obtain the plant data via digitalized human system interfaces (HSIs), large display panel (LDP), computerized procedure system (CPS), soft controls and other system features. Accordingly, the necessity to consider the new environment of MCR has been raised when performing human reliability analysis (HRA) methods. However, none of HRA methods consider the newly installed MCRs. The goal of this paper is to propose new frameworks to assess the human error probabilities (HEPs) in digitalized MCR. Generally, the HEPs are obtained by summing the probabilities of diagnosis errors and the probabilities of execution errors and those probabilities are modified by performance shaping factors (PSFs). In this paper, two parts are included; (1) Assessing the probabilities of diagnosis errors, and (2) Assessing the probabilities of execution errors. In this paper, the process to develop the frameworks are introduced and we expect that the HEPs affecting the features of digitalized MCR can be calculated by using the proposed frameworks. (authors)

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Optimization of human factors methods within the design cycle - 178

Amec Foster Wheeler developed and implemented the Human Factors Engineering program to analyze the design of a large-scale electrical component readying for installation in a nuclear facility. The HFE program applied the program elements from NUREG-0711 throughout the detailed design phase of the project. This paper provides: (1) a review of the Human Factors Engineering (HFE) program methodology that was implemented, and (2) a summary of the integrated approach to the design, verification, and validation of the Human-Machine Interface (HMI) display and mimic panel. In general, it was found that a collaborative approach between the design team, equipment vendor, HFE, and the end-users of the component created a more streamlined approach to the design and allowed for user feedback to be implemented throughout the progression of the design. However, the trade-off to this approach is that an integrated validation is likely required once all design aspects are complete, to ensure the modification is acceptable in the context of the overall system. (authors)

42 ENGINEERING↗

An overview of the IEEE human factors standard development activities - 2017 - 230

Since 1980 the Institute of Electrical and Electronics Engineers (IEEE) has supported development of human factors (HF) standards. Within IEEE, Subcommittee 5 (SC5) of the Nuclear Power Engineering Committee develops and maintains HF standards applicable to nuclear facilities. These standards are structured in a hierarchical fashion. The top-level standard defines the HF tasks required to support the integration of human performance into the design process. Five lower tier documents expand upon the upper tier standard. Presently, two new HF standards projects are underway; one to provide HF guidance for the validation of the system interface design and integrated systems operation and another for designing and developing computer-based displays for monitoring and control of nuclear facilities. In addition to producing and maintaining HF standards, SC5 is also involved in outreach activities, including sponsorship of a series of conferences on human factors and nuclear power plants. (authors)

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

A review of human-system interface design issues observed during analog-to-digital and digital-to-digital migrations in U.S. nuclear power plants - 312

The United States (U.S.) Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) program is developing a scientific basis through targeted research and development (R and D) to support the U.S. nuclear power plant (NPP) fleet in extending their existing licensing period and ensuring their long-term reliability, productivity, safety, and security. Over the last several years, human factors engineering (HFE) professionals at the Idaho National Laboratory (INL) have supported the LWRS Advanced Instrumentation, Information, and Control (II and C) System Technologies pathway across several U.S. commercial NPPs in analog-to-digital migrations (i.e., turbine control systems) and digital-to-digital migrations (i.e., Safety Parameter Display System). These efforts have included in-depth human factors evaluation of proposed human-system interface (HSI) design concepts against established U.S. Nuclear Regulatory Commission (NRC) design guidelines from NUREG-0700, Rev 2 to inform subsequent HSI design prior to transitioning into Verification and Validation. This paper discusses some of the overarching design issues observed from these past HFE evaluations. In addition, this work presents some observed challenges such as common tradeoffs utilities are likely to face when introducing new HSI technologies into NPP hybrid control rooms. The primary purpose of this work is to distill these observed design issues into general HSI design guidance that industry can use in early stages of HSI design. (authors)

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Study on main control room and human-system interface of generation III in China - 313

This paper focuses on the study on Main Control Room (MCR) and Human-System Interface (HSI) of Generation III in China. An intelligent HSI has been developed to enhance the safety and availability of Nuclear Power Plant (NPP) by improving operational reliability. The key elements of the proposed HSI such as Advanced Alarm System, State Oriented Procedures (SOP), Large Display Panel (LDP), Computerized-base procedures..., that affects what information the operator sees or the system's response to a control input must be empirically evaluated to ensure that the new design does not compromise HSI effectiveness. Based on CPR1000's experience in China, this paper presents the approach used as well as the most relevant aspects of this kind of project for the next generation. This approach will be used in new Generation III nuclear power plant, and also used in modernizing I and C system in currently operative nuclear power plants, in addition to meeting safety requirements and the plant's operational requirements, to improve cost-effective plant and human performance and to reduce likelihood of human errors, to gain maximum benefit of the implemented technology and to increase the performance, resulting in improved plant safety, availability, reliability, and cost-effective operation. The proposed HSI has been validated and demonstrated using the CPR1000 simulator of National Key Laboratory. After sufficient validation, the characteristic design features of the proposed HSI will be reflected in the design of the main control room of the next generation reactors in China. (authors)

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Monoamine oxidase: Radiotracer chemistry and human studies

Monoamine oxidase (MAO) oxidizes amines from both endogenous and exogenous sources thereby regulating the concentration of neurotransmitter amines such as serot onin, norepinephrine and dopamine as well as many xenobiotics. MAO inhibitor drugs are used in the treatment of Parkinson’s disease and in depression stimulating the development of radiotracer tools to probe the role of MAO in normal human biology and in disease. Over the past 30 since the first radiotracers were developed and the first PET images of MAO in humans were carried out, PET studies of brain MAO in healthy volunteers and in patients have identified different variables which have contributed to different MAO levels in brain and in peripheral organs. MAO radiotracers and PET have also been used to study the current and developing MAO inhibitor drugs including the selection of doses for clinical trials. In this article, we describe (1) the development of MAO radiotracers; (2) human studies including the relationship of brain MAO levels to genotype, personality, neurological and psychiatric disorders; (3) examples of the use of MAO radiotracers in drug research and development. We will conclude with outstanding needs to improve the radiotracers which are currently used and possible new applications.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

Using the Human Systems Simulation Laboratory at Idaho National Laboratory for Safety Focused Research

Under the United States (U.S.) Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) program, researchers at Idaho National Laboratory (INL) have been using the Human Systems Simulation Laboratory (HSSL) to conduct critical safety focused Human Factors research and development (R&D) for the nuclear industry. The LWRS program has the overall objective to develop the scientific basis to extend existing nuclear power plant (NPP) operating life beyond the current 60-year licensing period and to ensure their long-term reliability, productivity, safety, and security. One focus area for LWRS is the NPP main control room (MCR), because many of the instrumentation and control (I&C) system technologies installed in the MCR, while highly reliable and safe, are now difficult to replace and are therefore limiting the operating life of the NPP. This paper describes how INL researchers use the HSSL to conduct Human Factors R&D on modernizing or upgrading these I&C systems in a step-wise manner, and how the HSSL has addressed a significant gap in how to upgrade systems and technologies that are built to last, and therefore require careful integration of analog and new advanced digital technologies.

99 GENERAL AND MISCELLANEOUS↗

Invasion ecology of wild pigs (Sus scrofa) in Florida, USA: the role of humans in the expansion and colonization of an invasive wild ungulate

Wild pigs (Sus scrofa) are the most widely distributed invasive wild ungulate in the United States, yet the factors that influence wild pig dispersal and colonization at the regional level are poorly understood. Our objective was to use a population genetic approach to describe patterns of dispersal and colonization among populations to gain a greater understanding of the invasion process contributing to the expansion of this species. We used 52 microsatellite loci to produce individual genotypes for 482 swine sampled at 39 locations between 2014 and 2016. Our data revealed the existence of genetically distinct subpopulations (F ST = 0.1170, p < 0.05). We found evidence of both fine-scale subdivision among the sampling locations, as well as evidence of long term genetic isolation. Several locations exhibited significant admixture (interbreeding) suggesting frequent mixing of individuals among locations; up to 14% of animals were immigrants from other populations. This pattern of admixture suggested successive rounds of human-assisted translocation and subsequent expansion across Florida. We also found evidence of genetically distinct populations that were isolated from nearby populations, suggesting recent introduction by humans. In addition, proximity to wild pig holding facilities was associated with higher migration rates and admixture, likely due to the escape or release of animals. Taken together, these results suggest that human-assisted movement plays a major role in the ecology and rapid population growth of wild pigs in Florida.

59 BASIC BIOLOGICAL SCIENCES↗

The Earth has humans, so why don’t our climate models?

While climate models have rapidly advanced in sophistication over recent decades, they lack dynamic representation of human behavior and social systems despite strong feedbacks between social processes and climate. The impacts of climate change alter perceptions of risk and emissions behavior that, in turn, influence the rate and magnitude of climate change. Addressing this deficiency in climate models requires a substantial interdisciplinary effort to couple models of climate and human behavior. We suggest a multi-model approach that considers a range of theories and implementations of human behavior and social systems, similar to the multi-model approach that has been used to explore the physical climate system. In this work, we describe the importance of linking social factors with climate processes and identify four priorities essential to advancing the development of coupled social-climate models.

54 ENVIRONMENTAL SCIENCES↗

Cadmium induces cytotoxicity in human bronchial epithelial cells through upregulation of eIF5A1 and NF-kappaB

Highlights: • Normal human bronchial epithelial cells (BEAS-2B) were dosed with cadmium (Cd). • A low level (2 μM) of Cd treatment for 36 h elicited negligible cytotoxicity. • High levels (20 or 30 μM) of Cd treatment for 36 h induced cell death. • High levels of Cd can upregulate the protein levels of eIF5A1 and NF-κB p65. • We suggest that eIF5A1 level is possibly modulated by NF-κB. - Abstract: Cadmium (Cd) and Cd compounds are widely-distributed in the environment and well-known carcinogens. Here, we report that in CdCl{sub 2}-exposed human bronchial epithelial cells (BEAS-2B), the level of p53 is dramatically decreased in a time- and dose-dependent manner, suggesting that the observed Cd-induced cytotoxicity is not likely due to the pro-apoptotic function of p53. Therefore, this prompted us to further study the responsive pro-apoptotic factors by proteomic approaches. Interestingly, we identified that high levels (20 or 30 μM) of Cd can significantly upregulate the protein levels of eukaryotic translation initiation factor 5A1 (eIF5A1) and redox-sensitive transcription factor NF-κB p65. Moreover, there is an enhanced NF-κB nuclear translocation as well as chromatin-binding in Cd-treated BEAS-2B cells. We also show that small interfering RNA-specific knockdown of eIF5A1 in Cd-exposed cells attenuated the Cd cytotoxicity, indicating the potential role of eIF5A1 in Cd cytotoxicity. As eIF5A1 is reported to be related with cell apoptosis but little is known about its transcriptional control, we hypothesize that NF-κB might likely modulate eIF5A1 gene expression. Notably, by bioinformatic analysis, several potential NF-κB binding sites on the upstream promoter region of eIF5A1 gene can be found. Subsequent chromatin immunoprecipitation assay revealed that indeed there is enhanced NF-κB binding on eIF5A1 promoter region of Cd-treated BEAS-2B cells. Taken together, our findings suggest for the first time a regulatory mechanism for the pro-apoptotic protein eIF5A1 in which its level is possibly modulated by NF-κB in human lung cells.

60 APPLIED LIFE SCIENCES↗

Tetraspanin CD9 modulates human lymphoma cellular proliferation via histone deacetylase activity

Highlights: • CD9 is differentially expressed in human Burkitt’s lymphoma cells. • We found that CD9 expression promotes these cells proliferation. • CD9 expression also increases HDAC activity. • HDAC inhibition decreased both cell proliferation and importantly CD9 expression. • CD9 may dictate HDAC efficacy and play a role in HDAC regulation. - Abstract: Non-Hodgkin Lymphoma (NHL) is a type of hematological malignancy that affects two percent of the overall population in the United States. Tetraspanin CD9 is a cell surface protein that has been thoroughly demonstrated to be a molecular facilitator of cellular phenotype. CD9 expression varies in two human lymphoma cell lines, Raji and BJAB. In this report, we investigated the functional relationship between CD9 and cell proliferation regulated by histone deacetylase (HDAC) activity in these two cell lines. Introduction of CD9 expression in Raji cells resulted in significantly increased cell proliferation and HDAC activity compared to Mock transfected Raji cells. The increase in CD9–Raji cell proliferation was significantly inhibited by HDAC inhibitor (HDACi) treatment. Pretreatment of BJAB cells with HDAC inhibitors resulted in a significant decrease in endogenous CD9 mRNA and cell surface expression. BJAB cells also displayed decreased cell proliferation after HDACi treatment. These results suggest a significant relationship between CD9 expression and cell proliferation in human lymphoma cells that may be modulated by HDAC activity.

60 APPLIED LIFE SCIENCES↗

Differential expression of extracellular-signal-regulated kinase 5 (ERK5) in normal and degenerated human nucleus pulposus tissues and cells

Highlights: • ERK5 involved in NP cells. • ERK5 involved in NP tissue. • It was important modulator. - Abstract: Extracellular-signal-regulated kinase 5 (ERK5) is a member of the mitogen-activated protein kinase (MAPK) family and regulates a wide variety of cellular processes such as proliferation, differentiation, necrosis, apoptosis and degeneration. However, the expression of ERK5 and its role in degenerated human nucleus pulposus (NP) is hitherto unknown. In this study, we observed the differential expression of ERK5 in normal and degenerated human nucleus pulposus tissues by using immunohistochemical staining and Western blot. Treatment of NP cells with Pro-inflammatory cytokine, TNF-α decreased ERK5 gene expression as well as NP marker gene expression; including the type II collagen and aggrecan. Suppression of ERK5 gene expression in NP cells by ERK5 siRNA resulted in decreased gene expression of type II collagen and aggrecan. Furthermore, inhibition of ERK5 activation by BIX02188 (5 μM) decreased the gene expression of type II collagen and aggrecan in NP cells. Our results document the expression of ERK5 in degenerated nucleus pulposus tissues, and suggest a potential involvement of ERK5 in human degenerated nucleus pulposus.

60 APPLIED LIFE SCIENCES↗

Characterization of the human oncogene SCL/TAL1 interrupting locus (Stil) mediated Sonic hedgehog (Shh) signaling transduction in proliferating mammalian dopaminergic neurons

Highlights: • Stil is a human oncogene that is conserved in vertebrate species. • Stil functions in the Shh pathway in mammalian cells. • The expression of Stil is required for mammalian dopaminergic cell proliferation. - Abstract: The human oncogene SCL/TAL1 interrupting locus (Stil) is highly conserved in all vertebrate species. In humans, the expression of Stil is involved in cancer cell survival, apoptosis and proliferation. In this research, we investigated the roles of Stil expression in cell proliferation of mammalian dopaminergic (DA) PC12 cells. Stil functions through the Sonic hedgehog (Shh) signal transduction pathway. Co-immunoprecipitation tests revealed that STIL interacts with Shh downstream components, which include SUFU and GLI1. By examining the expression of Stil, Gli1, CyclinD2 (cell-cycle marker) and PCNA (proliferating cell nuclear antigen), we found that up-regulation of Stil expression (transfection with overexpression plasmids) increased Shh signaling transduction and PC12 cell proliferation, whereas down-regulation of Stil expression (by shRNA) inhibited Shh signaling transduction, and thereby decreased PC12 cell proliferation. Transient transfection of PC12 cells with Stil knockdown or overexpression plasmids did not affect PC12 cell neural differentiation, further indicating the specific roles of Stil in cell proliferation. The results from this research suggest that Stil may serve as a bio-marker for neurological diseases involved in DA neurons, such as Parkinson’s disease.

60 APPLIED LIFE SCIENCES↗

Human monoclonal antibodies derived from a patient infected with 2009 pandemic influenza A virus broadly cross-neutralize group 1 influenza viruses

Highlights: • Influenza infection can elicit heterosubtypic antibodies to group 1 influenza virus. • Three human monoclonal antibodies were generated from an H1N1-infected patient. • The antibodies predominantly recognized α-helical stem of viral hemagglutinin (HA). • The antibodies inhibited HA structural activation during the fusion process. • The antibodies are potential candidates for future antibody therapy to influenza. - Abstract: Influenza viruses are a continuous threat to human public health because of their ability to evolve rapidly through genetic drift and reassortment. Three human monoclonal antibodies (HuMAbs) were generated in this study, 1H11, 2H5 and 5G2, and they cross-neutralize a diverse range of group 1 influenza A viruses, including seasonal H1N1, 2009 pandemic H1N1 (H1N1pdm) and avian H5N1 and H9N2. The three HuMAbs were prepared by fusing peripheral blood lymphocytes from an H1N1pdm-infected patient with a newly developed fusion partner cell line, SPYMEG. All the HuMAbs had little hemagglutination inhibition activity but had strong membrane-fusion inhibition activity against influenza viruses. A protease digestion assay showed the HuMAbs targeted commonly a short α-helix region in the stalk of the hemagglutinin. Furthermore, Ile45Phe and Glu47Gly double substitutions in the α-helix region made the HA unrecognizable by the HuMAbs. These two amino acid residues are highly conserved in the HAs of H1N1, H5N1 and H9N2 viruses. The HuMAbs reported here may be potential candidates for the development of therapeutic antibodies against group 1 influenza viruses.

60 APPLIED LIFE SCIENCES↗

Arsenic trioxide inhibits cell proliferation and human papillomavirus oncogene expression in cervical cancer cells

Highlights: • As{sub 2}O{sub 3} inhibits growth of cervical cancer cells and expression of HPV oncogenes in these cells. • HPV-negative cervical cancer cells are more sensitive to As{sub 2}O{sub 3} than HPV-positive cervical cancer cells. • HPV-18 positive cervical cancer cells are more sensitive to As{sub 2}O{sub 3} than HPV-16 positive cancer cells. • Down-regulation of HPV oncogenes by As{sub 2}O{sub 3} is partially due to the diminished AP-1 binding. - Abstract: Arsenic trioxide (As{sub 2}O{sub 3}) has shown therapeutic effects in some leukemias and solid cancers. However, the molecular mechanisms of its anticancer efficacy have not been clearly elucidated, particularly in solid cancers. Our previous data showed that As{sub 2}O{sub 3} induced apoptosis of human papillomavirus (HPV) 16 DNA-immortalized human cervical epithelial cells and cervical cancer cells and inhibited the expression of HPV oncogenes in these cells. In the present study, we systemically examined the effects of As{sub 2}O{sub 3} on five human cervical cancer cell lines and explored the possible molecular mechanisms. MTT assay showed that HPV-negative C33A cells were more sensitive to growth inhibition induced by As{sub 2}O{sub 3} than HPV-positive cervical cancer cells, and HPV 18-positive HeLa and C4-I cells were more sensitive to As{sub 2}O{sub 3} than HPV 16-positive CaSki and SiHa cells. After As{sub 2}O{sub 3} treatment, both mRNA and protein levels of HPV E6 and E7 obviously decreased in all HPV positive cell lines. In contrast, p53 and Rb protein levels increased in all tested cell lines. Transcription factor AP-1 protein expression decreased significantly in HeLa, CaSki and C33A cells with ELISA method. These results suggest that As{sub 2}O{sub 3} is a potential anticancer drug for cervical cancer.

60 APPLIED LIFE SCIENCES↗