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NASA NTRS1946

Flight Investigation of Effect of Various Vertical-Tail Modifications on the Directional Stability and Control Characteristics of the P-63A-1 Airplane (AAF No. 42-68889)

Because the results of preliminary flight tests had indicated. the P-63A-1 airplane possessed insufficient directional stability, the NACA and the manufacturer (Bell Aircraft Corporation) suggested three vertical-tail modifications to remedy the deficiencies in the directional characteristics. These modifications included an enlarged vertical tail formed by adding a tip extension to the original vertical tail, a large sharp-edge ventral fin, and a small dorsal fin. The enlarged vertical tail involved only a slight increase in total vertical-tail area from 23.73 to 26.58 square feet but a relatively much larger increase in geometric aspect ratio from 1.24 to 1.73 based on height and area above the horizontal tail. At the request of the Air Material Command, Army Air Forces, flight tests were made to determine the effect of these modifications and of some combinations of these modifications on the directional stability and control characteristics of the airplane, In all, six different vertical-tail. configurations were investigated to determine the lateral and directional oscillation characteristics of the airplane, the sideslip characteristics, the yaw due to ailerons in rudder-fixed rolls from turns and pull-outs, the trim changes due to speed changes; and the trim changes due to power changes. Results of the tests showed that the enlarged vertical tail approximately doubled the directional stability of the airplane and that the pilots considered the directional stability provided by the enlarged vertical tail to be satisfactory. Calculations based on sideslip data obtained at an indicated airspeed of 300 miles per hour showed that the directional stability of the airplane with the original vertical tail corresponded to a value of 0(sub n beta) of -0.00056 whereas for the enlarged vertical tail the estimated va1ue of C(sub n beta) was -0.00130, The ventral fin was found to increase by a moderate amount the directional stability of the airplane with the original vertical tail for smal1 sides1ip angles at low speeds but little consistent change in directional stability was effected by the ventral fin at higher speeds, The effectiveness of the ventral fin was generally much less when used with the enlarged vertical tail than when used with the original vertical tail. The ventral and dorsal fins were found to be very effective in eliminating rudder-force reversals which occurred in low-speed, high-engine-power, sideslipped conditions of flight . Sideslip tests at two altitudes for approximately the sane engine power and indicated airspeed showed that a small decrease in static directional stability occurred with increasing altitude and this decrease in stability was attributed to the increased propeller blade angles required at high altitudes. The variations of rudder pedal force with indicated airspeed using normal rated power and a constant rudder tab setting through the speed range were desirably small for all the configurations tested. The rudder pedal force changed by about 50 pounds for a power change from engine idling power, to normal rated power and this pedal force change was largely independent of airspeed or of vertical-tail configuration for the various configurations tested.

Johnson, Harold I.↗
NASA NTRS2010

On-Demand Urine Analyzer

A lab-on-a-chip was developed that is capable of extracting biochemical indicators from urine samples and generating their surface-enhanced Raman spectra (SERS) so that the indicators can be quantified and identified. The development was motivated by the need to monitor and assess the effects of extended weightlessness, which include space motion sickness and loss of bone and muscle mass. The results may lead to developments of effective exercise programs and drug regimes that would maintain astronaut health. The analyzer containing the lab-on-a- chip includes materials to extract 3- methylhistidine (a muscle-loss indicator) and Risedronate (a bone-loss indicator) from the urine sample and detect them at the required concentrations using a Raman analyzer. The lab-on- a-chip has both an extractive material and a SERS-active material. The analyzer could be used to monitor the onset of diseases, such as osteoporosis.

Farquharson, Stuart↗
NASA NTRS2008

Formation of Large (Approximately 100 micrometers) Ice Crystals Near the Tropical Tropopause

Recent high-altitude aircraft measurements with in situ imaging instruments indicated the presence of relatively large (approx.100 microns length), thin (aspect ratios of approx.6:1 or larger) hexagonal plate ice crystals near the tropical tropopause in very low concentrations (<0.01/L). These crystals were not produced by deep convection or aggregation. We use simple growth-sedimentation calculations as well as detailed cloud simulations to evaluate the conditions required to grow the large crystals. Uncertainties in crystal aspect ratio leave a range of possibilities, which could be constrained by knowledge of the water vapor concentration in the air where the crystal growth occurred. Unfortunately, water vapor measurements made in the cloud formation region near the tropopause with different instruments ranged from <2 ppmv to approx.3.5 ppmv. The higher water vapor concentrations correspond to very large ice supersaturations (relative humidities with respect to ice of about 200%). If the aspect ratios of the hexagonal plate crystals are as small as the image analysis suggests (6:1, see companion paper (Lawson et al., 2008)) then growth of the large crystals before they sediment out of the supersaturated layer would only be possible if the water vapor concentration were on the high end of the range indicated by the different measurements (>3 ppmv). On the other hand, if the crystal aspect ratios are quite a bit larger (approx.10:1), then H2O concentrations toward the low end of the measurement range (approx.2-2.5 ppmv) would suffice to grow the large crystals. Gravity-wave driven temperature and vertical wind perturbations only slightly modify the H2O concentrations needed to grow the crystals. We find that it would not be possible to grow the large crystals with water concentrations less than 2 ppmv, even with assumptions of a very high aspect ratio of 15 and steady upward motion of 2 cm/s to loft the crystals in the tropopause region. These calculations would seem to imply that the measurements indicating water vapor concentrations less than 2ppmv are implausible, but we cannot rule out the possibility that higher humidity prevailed upstream of the aircraft measurements and the air was dehydrated by the cloud formation. Simulations of the cloud formation with a detailed model indicate that homogeneous freezing should generate ice concentrations larger than the observed concentrations (20/L), and even concentrations as low as 20/L should have depleted the vapor in excess of saturation and prevented growth of large crystals. It seems likely that the large crystals resulted from ice nucleation on effective heterogeneous nuclei at low ice supersaturations. Improvements in our understanding of detailed cloud microphysical processes require resolution of the water vapor measurement discrepancies in these very cold, dry regions of the atmosphere.

Jensen, E. J.↗
NASA NTRS2004

Evidence for Differential Comminution/Aeolian Sorting and Chemical Weathering of Martian Soils Preserved in Mars Meteorite EET79001

Impact-melt glasses containing Martian atmospheric gases in Mars meteorite EET79001 are formed from Martian soil fines that had undergone meteoroid-comminution and aeolian sorting accompanied by chemical weathering near Mars surface. Using SiO2 and SO3 as proxy for silicates and salts respectively in Mars soils, we find that SiO2 and SO3 correlate negatively with FeO and MgO and positively with Al2O3 and CaO in these glasses, indicating that the mafic and felsic components are depleted and enriched relative to the bulk host (Lith A/B) respectively as in the case of Moon soils. Though the overall pattern of mineral fractionation is similar between the soil fines on Mars and Moon, the magnitudes of the enrichments/depletions differ between these sample-suites because of pervasive aeolian activity on Mars. In addition to this mechanical processing, the Martian soil fines, prior to impact-melting, have undergone acid-sulfate dissolution under oxidizing/reducing conditions. The S03 content in EET79001,507 (Lith B) glass is approx.18% compared to < 2% in EET79001, 506 (Lith A). SiO2 and SO3 negatively correlate with each other in ,507 glasses similar to Pathfinder soils. The positive correlation found between FeO and SO3 in ,507 glasses as well as Pathfinder rocks and soils is consistent with the deposition of ferric-hydroxysulfate on regolith grains in an oxidizing environment. As in the case of Pathfinder soils, the Al 2O3 vs SiO2 positive correlation and FeO VS S102 negative correlation observed in ,507 glasses indicate that SiO2 from the regolith is mobilized as soluble silicic acid at low pH. The large off-set in the end-member FeO abundance ( SO3=0) between Pathfinder soil-free rock and sulfur-free rock in ,507 glass precursors suggests that the soils comprising the ,507 glasses contain much larger proportion of fine-grained Martian soil fraction that registers strong mafic depletion relative to Lith B. This inference is strongly supported by the Al2O3 - SO3 negative correlation observed in both ,507 glasses and pathfinder soils. Furthermore, the flat MgO-SO3 correlation observed in the case of ,507 glasses shows that the solubilized MgSO4 is mobilized by the aqueous solutions leaving behind the rock-residue with approx.2-3% MgO. This value is similar to the approx.2% MgO found for the soil-free rock at the Pathfinder site. The EET79001 ,506 glasses, in contrast, show that Al2O3 and CaO positively correlate with SO3 indicating that Al is precipitated as amorphous hydroxysulfate at relatively high pH. The FeO - SO3 negative correlation observed in ,506 glasses yields an end-member FeO abundance of approx.21% for the sulfur-free rock, which is consistent with the 22% FeO deduced for the Viking soil-free rock. Further, the FeO and MgO negative correlation with S03 observed in ,506 glasses indicates that the divalent Fe and Mg released from ferromagnesian minerals by acid sulfate dissolution are mobilized away from the reaction sites as soluble sulfates under reducing environment. A similar negative correlation between FeO and SO3 and a positive correlation between Al2O3 and SO3 found in Viking soils suggest that they also had undergone acid-sulfate dissolution under relatively reducing conditions.

Rao, M. N.↗
NASA NTRS2010

Separation of Lift-Generated Vortex Wakes Into Two Diverging Parts

As part of an ongoing study of the spreading rate of lift-generated vortex wakes, the present investigation considers possible reasons as to why segments of lift-generated wakes sometimes depart from the main part of the wake to move rapidly in either an upward or downward direction. It is assumed that deficiencies or enhancements of the lift carry over across the fuselage-shrouded wing are the driving mechanism for departures of wake-segments. The computations presented first indicate that upwardly departing wake segments that were observed and photographed could have been produced by a deficiency in lift carryover across the fuselage-shrouded part of the wing. Computations made of idealized vortex wakes indicate that upward departure of a wake segment requires a centerline reduction in the span loading of 70% or more, whether the engines are at idle or robust thrust. Similarly, it was found that downward departure of wake segments is produced when the lift over the center part of the wing is enhanced. However, it was also found that downward departures do not occur without the presence of robust engine-exhaust streams (i.e., engines must NOT be at idle). In those cases, downward departures of a wake segment occurs when the centerline value of the loading is enhanced by any amount between about 10% to 100%. Observations of condensation trails indicate that downward departure of wake segments is rare. Upward departures of wake segments appears to be more common but still rare. A study to determine the part of the aircraft that causes wake departures has not been carried out. However, even though departures of wake segments rarely occur, some aircraft do regularly shed these wake structures. If aircraft safety is to be assured to a high degree of reliability, and a solution for eliminating them is not implemented, existing guidelines for the avoidance of vortex wakes [1,3] may need to be broadened to include possible increases in wake sizes caused by vertical departures of wake segments. Further study may indicate that it is not possible to modify existing aircraft enough to prevent wake departures. Wake-avoidance guidelines must then be adjusted to provide the desired degree of safety. It appears that steps to avoid upwardly moving wake segments have already been incorporated into the avoidance procedures used for aircraft on approach to runways at the Frankfurt Airport [6,7]. The uncertainty in the prospects for compromises in flight safety caused by rapidly upwardly or downwardly moving wake segments suggest that it be specified that aircraft do not fly above or below each other during operations in the airport vicinity where aircraft are likely to be closely spaced [20].

Rossow, Vernon J.↗
NASA NTRS2010

Diurnal and Directional Responses of Chlorophyll Fluorescence and the PRI in a Cornfield

Determining the health and vigor of vegetation using high spectral resolution remote sensing is an important goal which has application to monitoring agriculture and ecosystem productivity and carbon exchange. Two spectral indices used to assess whether vegetation is performing near-optimally or exhibiting symptoms of environmental stress (e.g., drought or nutrient deficiency, non-optimal temperatures, etc.) are the Photochemical Reflectance Index (PRI) and solar-induced red and far-red Chlorophyll Fluorescence (Fs). Both the PRI and Fs capture the dynamics of photoprotection mechanisms within green foliage: the PRI is based on the association of the reflected radiation in the green spectrum with the xanthophyll cycle, whereas Fs measures the emitted radiation in the red and far-red spectrum. Fs was determined from retrievals in the atmospheric oxygen absorption features centered at 688 and 760 nm using a modified Fraunhofer Line Depth (FLD) method. We previously demonstrated diurnal and seasonal PRI differences for sunlit vs. shaded foliage in a conifer forest canopy, as expressed in the hotspot and darkspot of the Bidirectional Reflectance Function (BRF). In a USDA-ARS experimental field site located in Beltsville, MD, USA, measurements were acquired over a corn crop from a nadir view in 2008 with an ASD FieldSpec Pro (Analytical Spectral Devices, Inc., Boulder, CO, USA) to study the behavior of the PRI for sunlit and shaded foliage as captured in reflectance variations associated with the BRF, in a I m tall canopy in the vegetative growth stage. Those observations were compared to simulations obtained from two radiative transfer models. Measurements were then acquired to examine whether the PRI and Fs were influenced by view zenith and azimuth geometries at different times of day. Those measurements were made in 2010 with the Ocean Optics USB4000 Miniature Fiber Optic Spectrometer (Ocean Optics Inc., Dunedin, Florida, USA) at several times during the day on multiple days throughout the growing season. We found that the PRI consistently had higher values, indicating lower stress, in the BRF darkspot associated with shaded foliage than in the hotspot associated with sunlit foliage. We also found that Fs exhibited differences associated with sunlit and shaded canopy sectors, which were most pronounced for the red/far-red Fs ratio. Values indicated greater physiological stress in afternoons compared to mornings, and in the early senescent canopy as compared to the vegetative growth stage, BRFs for both the PRI and the red/far-red Fs ratio were bowl-shaped for the full azimuth sweep of the canopy. These two spectral indices (PRI, Fs ratio) provided complementary information on the photosynthetic function of the corn canopy.

Middleton, Elizabeth↗
NASA NTRS2009

Chapter 13. Atmospheric Dynamics and Meteorology

Titan, after Venus, is the second example in the solar system of an atmosphere with a global cyclostrophic circulation, but in this case a circulation that has a strong seasonal modulation in the middle atmosphere. Direct measurement of Titan's winds, particularly observations tracking the Huygens probe at 10 deg S, indicate that the zonal winds are mostly in the sense of the satellite's rotation. They generally increase with altitude and become cyclostrophic near 35 km above the surface. An exception to this is a sharp minimum centered near 75 km, where the wind velocity decreases to nearly zero. Zonal winds derived from temperatures retrieved from Cassini orbiter measurements, using the thermal wind equation, indicate a strong winter circumpolar vortex, with maximum winds of 190 m/s at mid northern latitudes near 300 km. Above this level, the vortex decays. Curiously, the stratospheric zonal winds and temperatures in both hemispheres are symmetric about a pole that is offset from the surface pole by about 4 deg. The cause of this is not well understood, but it may reflect the response of a cyclostrophic circulation to the onset between the equator, where the distance to the rotation axis is greatest, and the seasonally varying subsolar latitude. The mean meridional circulation can be inferred from the temperature field and the meridional distribution of organic molecules and condensates and hazes. Both the warm temperatures near 400 km and the enhanced concentration of several organic molecules suggest subsidence in the north polar region during winter and early spring. Stratospheric condensates are localized at high northern latitudes, with a sharp cut-off near 50 deg N. Titan's winter polar vortex appears to share many of the same characteristics of isolating high and low-latitude air masses as do the winter polar vortices on Earth that envelop the ozone holes. Global mapping of temperatures, winds, and composition in the troposphere, by contrast, is incomplete. The few suitable discrete clouds that have been found for tracking indicate smaller velocities than aloft, consistent: with the Huygens measurements, Along the descent trajectory, the Huygens measurements indicate eastward zonal winds down to 7 km, where they shift westward, and then eastward again below 1 km dawn to the surface. The low-latitude dune fields seen in Cassini RADAR images have been interpreted as longitudinal dunes occurring in a mean eastward zonal wind. This is not like Earth, where the low-latitude winds are westward above the surface. Because the net zonal-mean time-averaged torque exerted by the surface on the atmosphere should vanish, there must be westward flow over part of the surface; the question is where and when. The meridional contrast in tropospheric temperatures deduced from radio occultations at low, mid, and high latitudes. is small, approximately 5 K at the tropopause and approximately 3 K at the surface. This implies efficient heat transport, probably by axisymmetric meridional circulations. The effect of the methane "hydrological" cycle on the atmospheric circulation is not well constrained by existing measurements, Understanding the mature of the surface-atmosphere coupling will be critical to elucidating the atmospheric transports of momentum, heat, and volatiles.

Flasar, F. M.↗
NASA NTRS2009

On the Importance of Small Ice Crystals in Tropical Anvil Cirrus

In situ measurements of ice crystal concentrations and sizes made with aircraft instrumentation over the past two decades have often indicated the presence of numerous relatively small (< 50 m diameter) crystals in cirrus clouds. Further, these measurements frequently indicate that small crystals account for a large fraction of the extinction in cirrus clouds. The fact that the instruments used to make these measurements, such as the Forward Scattering Spectrometer Probe (FSSP) and the Cloud Aerosol Spectrometer (CAS), ingest ice crystals into the sample volume through inlets has led to suspicion that the indications of numerous small ]crystals could be artifacts of large ]crystal shattering on the instrument inlets. We present new aircraft measurements in anvil cirrus sampled during the Tropical Composition, Cloud, and Climate Coupling (TC4) campaign with the 2 ] Dimensional Stereo (2D ]S) probe, which detects particles as small as 10 m. The 2D ]S has detector "arms" instead of an inlet tube. Since the 2D ]S probe surfaces are much further from the sample volume than is the case for the instruments with inlets, it is expected that 2D ]S will be less susceptible to shattering artifacts. In addition, particle inter ]arrival times are used to identify and remove shattering artifacts that occur even with the 2D ]S probe. The number of shattering artifacts identified by the 2D ]S interarrival time analysis ranges from a negligible contribution to an order of magnitude or more enhancement in apparent ice concentration over the natural ice concentration, depending on the abundance of large crystals and the natural small ]crystal concentration. The 2D ]S measurements in tropical anvil cirrus suggest that natural small ]crystal concentrations are typically one to two orders of magnitude lower than those inferred from CAS. The strong correlation between the CAS/2D ]S ratio of small ]crystal concentrations and large ]crystal concentration suggests that the discrepancy is likely caused by shattering of large crystals on the CAS inlet. We argue that past measurements with CAS in cirrus with large crystals present may contain errors due to crystal shattering, and past conclusions derived from these measurements may need to be revisited. Further, we present correlations between CAS spurious concentration and 2D ]S large ]crystal mass from spatially uniform anvil cirrus sampling periods as an approximate guide for estimating quantitative impact of large ]crystal shattering on CAS concentrations in previous datasets. We use radiative transfer calculations to demonstrate that in the maritime anvil cirrus sampled during TC4, small crystals indicated by 2D ]S contribute relatively little cloud extinction, radiative forcing, or radiative heating in the anvils, regardless of anvil age or vertical location in the clouds. While 2D ]S ice concentrations in fresh anvil cirrus may often exceed 1 cm.3, and are observed to exceed 10 cm.3 in turrets, they are typically ~0.1 cm.3 and rarely exceed 1 cm.3 (<1.4% of the time) in aged anvil cirrus. We hypothesize that isolated occurrences of higher ice concentrations in aged anvil cirrus may be caused by ice nucleation driven by either small ]scale convection or gravity waves. It appears that the numerous small crystals detrained from convective updrafts do not persist in the anvil cirrus sampled during TC ]4.

Jensen, E. J.↗
NASA NTRS2011

A Multifunctional Coating for Autonomous Corrosion Control

Nearly all metals and their alloys are subject to corrosion that causes them to lose their structural integrity or other critical functionality. Protective coatings are the most commonly used method of corrosion control. However, progressively stricter environmental regulations have resulted in the ban of many commercially available corrosion protective coatings due to the harmful effects of their solvents or corrosion inhibitors. This work concerns the development of a multifunctional smart coating for the autonomous control of corrosion. This coating is being developed to have the inherent ability to detect the chemical changes associated with the onset of corrosion and respond autonomously to indicate it and control it. The multi-functionality of the coating is based on microencapsulation technology specifically designed for corrosion control applications. This design has, in addition to all the advantages of existing microcapsulation designs, the corrosion controlled release function that triggers the delivery of corrosion indicators and inhibitors on demand, only when and where needed. Microencapsulation of self-healing agents for autonomous repair of mechanical damage to the coating is also being pursued. Corrosion indicators, corrosion inhibitors, as well as self-healing agents, have been encapsulated and dispersed into several paint systems to test the corrosion detection, inhibition, and self-healing properties of the coating. Key words: Corrosion, coating, autonomous corrosion control, corrosion indication, corrosion inhibition, self-healing coating, smart coating, multifunctional coating, microencapsulation.

Calle, Luz M.↗
NASA NTRS2011

Using Enhanced Grace Water Storage Data to Improve Drought Detection by the U.S. and North American Drought Monitors

NASA's Gravity Recovery and Climate Experiment (GRACE) satellites measure time variations of the Earth's gravity field enabling reliable detection of spatio-temporal variations in total terrestrial water storage (TWS), including groundwater. The U.S. and North American Drought Monitors rely heavily on precipitation indices and do not currently incorporate systematic observations of deep soil moisture and groundwater storage conditions. Thus GRACE has great potential to improve the Drought Monitors by filling this observational gap. GRACE TWS data were assimilating into the Catchment Land Surface Model using an ensemble Kalman smoother enabling spatial and temporal downscaling and vertical decomposition into soil moisture and groundwater components. The Drought Monitors combine several short- and long-term drought indicators expressed in percentiles as a reference to their historical frequency of occurrence. To be consistent, we generated a climatology of estimated soil moisture and ground water based on a 60-year Catchment model simulation, which was used to convert seven years of GRACE assimilated fields into drought indicator percentiles. At this stage we provide a preliminary evaluation of the GRACE assimilated moisture and indicator fields.

Houborg, Rasmus↗
NASA NTRS2010

The Response of African Land Surface Phenology to Large Scale Climate Oscillations

Variations in agricultural production due to rainfall and temperature fluctuations are a primary cause of food insecurity on the African continent. Analysis of changes in phenology can provide quantitative information on the effect of climate variability on growing seasons in agricultural regions. Using a robust statistical methodology, we describe the relationship between phenology metrics derived from the 26 year AVHRR NDVI record and the North Atlantic Oscillation index (NAO), the Indian Ocean Dipole (IOD), the Pacific Decadal Oscillation (PDO), and the Multivariate ENSO Index (MEI). We map the most significant positive and negative correlation for the four climate indices in Eastern, Western and Southern Africa between two phenological metrics and the climate indices. Our objective is to provide evidence of whether climate variability captured in the four indices has had a significant impact on the vegetative productivity of Africa during the past quarter century. We found that the start of season and cumulative NDVI were significantly affected by large scale variations in climate. The particular climate index and the timing showing highest correlation depended heavily on the region examined. In Western Africa the cumulative NDVI correlates with PDO in September-November. In Eastern Africa the start of the June-October season strongly correlates with PDO in March-May, while the PDO in December-February correlates with the start of the February-June season. The cumulative NDVI over this last season relates to the MEI of March-May. For Southern Africa, high correlations exist between SOS and NAO of September-November, and cumulative NDVI and MEI of March-May. The research shows that climate indices can be used to anticipate late start and variable vigor in the growing season of sensitive agricultural regions in Africa.

Brown, Molly E.↗
NASA NTRS2011

Assessing the Lifetime Performance of the Lightning Imaging Sensor (LIS): Implications for the Geostationary Lightning Mapper (GLM)

The Lightning Imaging Sensor (LIS) onboard the Tropical Rainfall Measuring Mission (TRMM) satellite has been providing observations of total lightning over the Earth s Tropics for 13 years. This study examines the performance of the LIS throughout its time in orbit. Application of the Deep Convective Cloud Technique (DCCT) (Doelling et al., 2004) was performed on the LIS background pixels to assess the stability of the LIS instrument. The DCCT analysis indicates that the maximum deviation of the monthly mean radiance is within 2% of the overall mean, indicating stable performance over the period. In addition, an examination of the number of flashes detected over time similarly shows no significant trend (after adjusting for the orbit boost that occurred in August 2001). These and other results indicate that there has been no discernible change in LIS performance throughout its lifetime. A similar approach will used for monitoring the performance of the Geostationary Lightning Mapper (GLM) onboard the next generation Geostationary Operational Environmental Satellite-R (GOES-R). Since GLM is based on LIS design heritage, the LIS results indicate that GLM may also experience stable performance over its lifetime.

Buechler, D. E.↗
NASA NTRS2011

ITCS Test Strip Development and Certification

Internal coolant loops used for International Space Station thermal control must be periodically monitored for system health, including pH, biocide levels and any indication of ammonia. The presence of ammonia, possible via a microleak in the interface between the internal and external thermal control systems, could be a danger to the crew. The Internal Thermal Control System (ITCS) Sampling Kit uses test strips as a colorimetric indicator of pH and concentrations of biocide and free ammonia. This paper describes the challenges in designing an ammonia colorimetric indicator in a variable pH environment, as well as lessons learned, ultimately resulting in a robust test strip to indicate a hazardous ammonia leak.

Carrigan, Caitlin↗
NASA NTRS2003

Quantitating Iron in Serum Ferritin by Use of ICP-MS

A laboratory method has been devised to enable measurement of the concentration of iron bound in ferritin from small samples of blood (serum). Derived partly from a prior method that depends on large samples of blood, this method involves the use of an inductively-coupled-plasma mass spectrometer (ICP-MS). Ferritin is a complex of iron with the protein apoferritin. Heretofore, measurements of the concentration of serum ferritin (as distinguished from direct measurements of the concentration of iron in serum ferritin) have been used to assess iron stores in humans. Low levels of serum ferritin could indicate the first stage of iron depletion. High levels of serum ferritin could indicate high levels of iron (for example, in connection with hereditary hemochromatosis an iron-overload illness that is characterized by progressive organ damage and can be fatal). However, the picture is complicated: A high level of serum ferritin could also indicate stress and/or inflammation instead of (or in addition to) iron overload, and low serum iron concentration could indicate inflammation rather than iron deficiency. Only when concentrations of both serum iron and serum ferritin increase and decrease together can the patient s iron status be assessed accurately. Hence, in enabling accurate measurement of the iron content of serum ferritin, the present method can improve the diagnosis of the patient s iron status. The prior method of measuring the concentration of iron involves the use of an atomic-absorption spectrophotometer with a graphite furnace. The present method incorporates a modified version of the sample- preparation process of the prior method. First, ferritin is isolated; more specifically, it is immobilized by immunoprecipitation with rabbit antihuman polyclonal antibody bound to agarose beads. The ferritin is then separated from other iron-containing proteins and free iron by a series of centrifugation and wash steps. Next, the ferritin is digested with nitric acid to extract its iron content. Finally, a micronebulizer is used to inject the sample of the product of the digestion into the ICPMS for analysis of its iron content. The sensitivity of the ICP-MS is high enough to enable it to characterize samples smaller than those required in the prior method (samples can be 0.15 to 0.60 mL).

Smith, Scott M.↗
NASA NTRS2011

Use of Dual-Polarization Radar Variables to Assess Low-Level Wind Shear in Severe Thunderstorm Near-storm Environments in the Tennessee Valley

The upgrade of the National Weather Service (NWS) network of S ]band dual-polarization radars is currently underway, and the incorporation of polarimetric information into the real ]time forecasting process will enhance the forecaster fs ability to assess thunderstorms and their near ]storm environments. Recent research has suggested that the combination of polarimetric variables differential reflectivity (ZDR) and specific differential phase (KDP) can be useful in the assessment of low level wind shear within a thunderstorm. In an environment with strong low ]level veering of the wind, ZDR values will be largest along the right inflow edge of the thunderstorm near a large gradient in horizontal reflectivity (indicative of large raindrops falling with a relative lack of smaller drops), and take the shape of an arc. Meanwhile, KDP values, which are proportional to liquid water content and indicative of a large number of smaller drops, are maximized deeper into the forward flank precipitation shield than the ZDR arc as the smaller drops are being advected further from the updraft core by the low level winds than the larger raindrops. Using findings from previous work, three severe weather events that occurred in North Alabama were examined in order to assess the utility of these signatures in determining the potential for tornadic activity. The first case is from October 26, 2010, where a large number of storms indicated tornadic potential from a standard reflectivity and velocity analysis but very few storms actually produced tornadoes. The second event is from February 28, 2011, where tornadic storms were present early on in the event, but as the day progressed, the tornado threat transitioned to a high wind threat. The third case is from April 27, 2011, where multiple rounds of tornadic storms ransacked the Tennessee Valley. This event provides a dataset including multiple modes of tornadic development, including QLCS and supercell structures. The overarching goal of examining these three events is to compare dual ]polarization features from this larger dataset to previous work and to determine if these signatures can be a useful indication of the potential for tornadic activity associated with the amount of low ]level wind shear in the near ]storm environment.

Crowe, Christina C.↗
NASA NTRS2011

Microbial Characterization of Solid-Wastes Treated with Heat Melt Compaction Technology

The research purpose of the project was to determine the fate of microorganisms in space-generated solid wastes after processing by a Heat Melt Compactor (HMC), which is a candidate solid waste treatment technology. Five HMC product disks were generated at Ames Research Center (ARC), Waste Management Systems element. The feed for two was simulated space-generated trash and feed for three was Volume F compartment wet waste returned on STS 130. Conventional microbiological methods were used to detect and enumerate microorganisms in HMC disks and in surface swab samples of HMC hardware before and after operation. Also, biological indicator test strips were added to the STS trash prior to compaction to test if HMC processing conditions, 150 C for approx 3 hr and dehydration, were sufficient to eliminate the test bacteria on the strips. During sample acquisition at KSC, the HMC disk surfaces were sanitized with 70% alcohol to prevent contamination of disk interiors. Results from microbiological assays indicated that numbers of microbes were greatly reduced but not eliminated by the 70% alcohol. Ten 1.25 cm diameter cores were aseptically cut from each disk to sample the disk interior. The core material was run through the microbial characterization analyses after dispersal in sterile diluent. Low counts of viable bacteria (5 to 50 per core) were found but total direct counts were 6 to 8 orders of magnitude greater. These results indicate that the HMC operating conditions might not be sufficient for complete waste sterilization, but the vast majority of microbes present in the wastes were dead or non-cultivable after HMC treatment. The results obtained from analyses of the commercial spore test strips that had been added fo the wastes prior to HMC operation further indicated that the HMC was sterilizing the wastes. Nearly all strips were recovered from the HMC disks and all of these were negative for spore growth when run through the manufacturer's protocol. The 10(exp 6) or so spores impregnated into the strips were no longer viable. Control test strips, i.e., not exposed to the HMC conditions, were all strongly positive. All isolates from the cultivable counts were identified, leading to one concern: several were identified as Staphylococcus aureus, a human pathogen. The project reported here provides microbial characterization support to the Waste Management Systems element of the Life Support and Habitation Systems program.

Strayer, Richard F.↗
NASA NTRS2006

Evaluation of Mesoscale Model Phenomenological Verification Techniques

Forecasters at the Spaceflight Meteorology Group, 45th Weather Squadron, and National Weather Service in Melbourne, FL use mesoscale numerical weather prediction model output in creating their operational forecasts. These models aid in forecasting weather phenomena that could compromise the safety of launch, landing, and daily ground operations and must produce reasonable weather forecasts in order for their output to be useful in operations. Considering the importance of model forecasts to operations, their accuracy in forecasting critical weather phenomena must be verified to determine their usefulness. The currently-used traditional verification techniques involve an objective point-by-point comparison of model output and observations valid at the same time and location. The resulting statistics can unfairly penalize high-resolution models that make realistic forecasts of a certain phenomena, but are offset from the observations in small time and/or space increments. Manual subjective verification can provide a more valid representation of model performance, but is time-consuming and prone to personal biases. An objective technique that verifies specific meteorological phenomena, much in the way a human would in a subjective evaluation, would likely produce a more realistic assessment of model performance. Such techniques are being developed in the research community. The Applied Meteorology Unit (AMU) was tasked to conduct a literature search to identify phenomenological verification techniques being developed, determine if any are ready to use operationally, and outline the steps needed to implement any operationally-ready techniques into the Advanced Weather Information Processing System (AWIPS). The AMU conducted a search of all literature on the topic of phenomenological-based mesoscale model verification techniques and found 10 different techniques in various stages of development. Six of the techniques were developed to verify precipitation forecasts, one to verify sea breeze forecasts, and three were capable of verifying several phenomena. The AMU also determined the feasibility of transitioning each technique into operations and rated the operational capability of each technique on a subjective 1-10 scale: (1) 1 indicates that the technique is only in the initial stages of development, (2) 2-5 indicates that the technique is still undergoing modifications and is not ready for operations, (3) 6-8 indicates a higher probability of integrating the technique into AWIPS with code modifications, and (4) 9-10 indicates that the technique was created for AWIPS and is ready for implementation. Eight of the techniques were assigned a rating of 5 or below. The other two received ratings of 6 and 7, and none of the techniques a rating of 9-10. At the current time, there are no phenomenological model verification techniques ready for operational use. However, several of the techniques described in this report may become viable techniques in the future and should be monitored for updates in the literature. The desire to use a phenomenological verification technique is widespread in the modeling community, and it is likely that other techniques besides those described herein are being developed, but the work has not yet been published. Therefore, the AMIU recommends that the literature continue to be monitored for updates to the techniques described in this report and for new techniques being developed whose results have not yet been published. 111

Lambert, Winifred↗
NASA NTRS2012

Microbial Characterization Space Solid Wastes Treated with a Heat Melt Compactor

The on going purpose of the project efforts was to characterize and determine the fate of microorganisms in space-generated solid wastes before and after processing by candidate solid waste processing. For FY 11, the candidate technology that was assessed was the Heat Melt Compactor (HMC). The scope included five HMC. product disks produced at ARC from either simulated space-generated trash or from actual space trash, Volume F compartment wet waste, returned on STS 130. This project used conventional microbiological methods to detect and enumerate microorganisms in heat melt compaction (HMC) product disks as well as surface swab samples of the HMC hardware before and after operation. In addition, biological indicators were added to the STS trash prior to compaction in order to determine if these spore-forming bacteria could survive the HMC processing conditions, i.e., high temperature (160 C) over a long duration (3 hrs). To ensure that surface dwelling microbes did not contaminate HMC product disk interiors, the disk surfaces were sanitized with 70% alcohol. Microbiological assays were run before and after sanitization and found that sanitization greatly reduced the number of identified isolates but did not totally eliminate them. To characterize the interior of the disks, ten 1.25 cm diameter core samples were aseptically obtained for each disk. These were run through the microbial characterization analyses. Low counts of bacteria, on the order of 5 to 50 per core, were found, indicating that the HMC operating conditions might not be sufficient for waste sterilization. However, the direct counts were 6 to 8 orders of magnitude greater, indicating that the vast majority of microbes present in the wastes were dead or non-cultivable. An additional indication that the HMC was sterilizing the wastes was the results from the added commercial spore test strips to the wastes prior to HMC operation. Nearly all could be recovered from the HMC disks post-operation and all were showed negative growth when run through the manufacturer's protocol, meaning that the 106 or so spores impregnated into the strips were dead. Control test strips, i.e., not exposed to the HMC conditions were all strongly positive. One area of concern is that the identities of isolates from the cultivable counts included several human pathogens, namely Staphylococcus aureus. The project reported here provides microbial characterization support to the Waste Management Systems element of the Life Support and Habitation Systems program.

Strayer, Richard F.↗