Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “FISH”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,675 records · Page 93
All sourcesNASA NTRSDOE OSTI
NASA NTRS2003

STS-107 Flight Day 9 Highlights

This video shows the activities of the STS-107 crew (Rick Husband, Commander; William McCool, Pilot; Kalpana Chawla, David Brown, Michael Anderson, Laurel Clark, Mission Specialists; Ilan Ramon, Payload Specialist) during flight day 9 of the Columbia orbiter's final flight. The primary activities of flight day 9 are spaceborne experiments. The video shows a commercial experiment on roses, a partial view of Africa from Libya to the Horn of Africa through the MEIDEX (Mediterranean Israeli Dust Experiment), and the FAST (Facility for Absorption and Surface Tension) experiment. The STARS (Space Technology and Research Students) international student experiments are shown. The preliminary results of these experiments on the effects of microgravity on silkworms, spiders, crystal growth, fish embryos, carpenter bees, and ants are discussed. The video includes a view of southern Spain and the Mediterranean Sea.

Source record↗
NASA NTRS2003

STS-107 Flight Day 5 Highlights

The fifth day of the STS-107 space mission begins with a presentation of The Six Space Technology and Research Students (STARS) program experiments aboard the Space Shuttle Columbia. Students from Australia, China, Israel, Japan, Lichtenstein and The United States send scientific experiments into space. The video includes the progress of experiments with various insects including silkworms, carpenter bees, ants, fish, and spiders.

Source record↗
NASA NTRS2003

STS-107 Flight Day 12 Highlights

This video shows the activities of the STS-107 crew (Rick Husband, Commander; William McCool, Pilot; Kalpana Chawla, David Brown, Michael Anderson, Laurel Clark, Mission Specialists; Ilan Ramon, Payload Specialist) during flight day 12 of the Columbia orbiter's final mission. The primary activities are spaceborne experiments in the SpaceHab RDM (Research Double Module). Experiments shown in the video include SOFBALL (Structure of Flame Balls at Low Lewis-Number), an experiment to grow cancer cells in microgravity, and the STARS (Space Technology and Research Students) experiments, including bees, ants, chemical gardens, fish, and spiders. Crew Members are shown working on MIST (Water Mist Fire Suppression), a commercial experiment. Red Team crew members (Husband, Chawla, Clark, Ramon) are shown conversing through a handset with the Expedition 6 crew (Kenneth Bowersox, Commander; Donald Pettit, Nikolai Budarin; Flight Engineers) of the ISS (International Space Station).

Source record↗
NASA NTRS2003

STS-107 Flight Day 13 Highlights

This video shows the activities of the STS-107 crew on flight day 13 of the Columbia orbiter's final mission. The crew members include: Rick Husband, Commander; William McCool, Pilot; Kalpana Chawla, David Brown, Michael Anderson, Laurel Clark, Mission Specialists; Ilan Ramon, Payload Specialist. The primary activities of flight day 13 are spaceborne experiments, including troubleshooting undertaken by Mission Specialist Chawla on the Water Mist Fire Suppression (MIST) experiment. Chawla performs troubleshooting tasks relayed to her by Mission Control. She shows Mission Control the location of air and water in a transparent hose that is part of the atomizer on the exterior of the combustion module. She also changes the atomizer head. All six Space Technology and Research Students (STARS) experiments are profiled in the video. These experiments are on ants, crystal growth in a chemical garden, fish embryos, carpenter bees, spiders, and silkworms. The video also includes a view of the southeast Texas coast near Houston, and a view of Portugal, Spain, Gibraltar, Morocco, and the Sahara Desert. The video ends with an explanation of roses at Mission Control which commemorate astronauts who have died on missions.

Source record↗
NASA NTRS2002

A Perspective of Our Planet's Atmosphere, Land, and Oceans: A View from Space

A birds eye view of the Earth from afar and up close reveals the power and magnificence of the Earth and juxtaposes the simultaneous impacts and powerlessness of humankind. The NASA Electronic Theater presents Earth science observations and visualizations in true high definition (HD) format. See the latest spectacular images from NASA & NOAA remote sensing missions like GOES, TRMM, Landsat 7, QuikScat, and Terra, which will be visualized and explained in the context of global change. Marvel at visualizations of global data sets currently available from Earth orbiting satellites, including the Earth at night with its city lights, aerosols from biomass burning, and global cloud properties. See the dynamics of vegetation growth and decay over South America over 17 years, and its contrast to the North American and Africa continents. Spectacular new visualizations of the global atmosphere & oceans will be shown. See massive dust storms sweeping across Africa and across the Atlantic to the Caribbean and Amazon basin. See ocean vortexes and currents that bring up the nutrients to feed tiny phytoplankton and draw the fish, giant whales and fisher- man. See how the ocean blooms in response to these currents and El Nino/La Nina climate changes. We will illustrate these and other topics with a dynamic theater-style presentation, along with animations of satellite launch deployments and orbital mapping to highlight aspects of Earth observations from space.

King, Michael D.↗
NASA NTRS2003

STS-107 Mission Highlights Resource Tape, Part 1 of 4

This video, Part 1 of 4, shows the activities of the STS-107 crew during flight days 1 through 3 of the Columbia orbiter's final flight. The crew consists of Commander Rick Husband, Pilot William McCool, Payload Commander Michael Anderson, Mission Specialists David Brown, Kalpana Chawla, and Laurel Clark, and Payload Specialist Ilan Ramon. Before launch on flight day 1 the astronauts are seen at their pre-flight banquet, during suit-up, and while being seated on the orbiter. David Brown takes footage of the space shuttle's external tank after it is jettisoned. The video includes replays of the launch from several angles. The onboard views of launch are narrated by William McCool and Kalpana Chawla. On flight days 2 and 3 student microgravity experiments in the SpaceHab module in the shuttle's payload bay are profiled. These experiments address microgravity effects on crystal growth, ants, bees, fish embryos, silkworms, and spiders. Other experiments profiled include the Mediterranean Israeli Dust Experiment (MEIDEX), FAST (surface tension of bubbles), SOLS (Ozone), an experiment to culture prostate cancer cells in a bioreactor, and a commercial plant growth experiment. Earth views include lightning at night, and a view of the Strait of Gibraltar, including Spain and Morocco.

Source record↗
NASA NTRS2003

The NASA/NOAA Electronic Theater

The NASA/NOAA Electronic Theater presents Earth science observations and visualizations from space in a historical perspective. Fly in from outer space to Cambridge and Harvard University. Zoom through the Cosmos to SLC and site of the 2002 Winter Olympics using 1 m IKONOS "Spy Satellite" data. Contrast the 1972 Apollo 17 "Blue Marble" image of the Earth with the latest US and International global satellite images that allow us to view our Planet from any vantage point. See the latest spectacular images from NASA/NOAA remote sensing missions like Terra, GOES, TRMM, SeaWiFS, & Landsat 7, of storms & fires like Hurricane Isabel and the LNSan Diego firestorms of 2003. See how High Definition Television (HDTV) is revolutionizing the way we do science communication. Take the pulse of the planet on a daily, annual and 30-year time scale. See daily thunderstorms, the annual blooming of the northern hemisphere landmasses and oceans, fires in Africa, dust storms in Iraq, and carbon monoxide exhaust from global burning. See visualizations featured on Newsweek, TIME, National Geographic, Popular Science covers & National & International Network TV. Spectacular new global visualizations of the observed and simulated atmosphere & oceans are shown. See the currents and vortexes in the oceans that bring up the nutrients to feed tiny plankton and draw the fish, whales and fishermen. See the how the ocean blooms in response to El Niiioh Niiia climate changes. The Etheater will be presented using the latest High Definition TV (HDTV) and video projection technology on a large screen. See the global city lights, and the great NE US blackout of August 2003 observed by the "night-vision" DMSP satellite.

Hasler, A. F.↗
NASA NTRS2003

Bio-inspired Computing for Robots

Living creatures may provide algorithms to enable active sensing/control systems in robots. Active sensing could enable planetary rovers to feel their way in unknown environments. The surface of Jupiter's moon Europa consists of fractured ice over a liquid sea that may contain microbes similar to those on Earth. To explore such extreme environments, NASA needs robots that autonomously survive, navigate, and gather scientific data. They will be too far away for guidance from Earth. They must sense their environment and control their own movements to avoid obstacles or investigate a science opportunity. To meet this challenge, CICT's Information Technology Strategic Research (ITSR) Project is funding neurobiologists at NASA's Jet Propulsion Laboratory (JPL) and selected universities to search for biologically inspired algorithms that enable robust active sensing and control for exploratory robots. Sources for these algorithms are living creatures, including rats and electric fish.

Laufenberg, Larry↗
NASA NTRS2003

Truly incomplete and complex exchanges in prematurely condensed chromosomes of human fibroblasts exposed in vitro to energetic heavy ions

Confluent human fibroblast cells (AG1522) were irradiated with gamma rays, 490 MeV/nucleon silicon ions, or iron ions at either 200 or 500 MeV/nucleon. The cells were allowed to repair at 37 degrees C for 24 h after exposure, and a chemically induced premature chromosome condensation (PCC) technique was used to condense chromosomes in the G2 phase of the cell cycle. Incomplete and complex exchanges were analyzed in the irradiated samples. To verify that chromosomal breaks were truly unrejoined, chromosome aberrations were analyzed using a combination of whole-chromosome specific probes and probes specific for the telomere region of the chromosome. Results showed that the frequency of unrejoined chromosome breaks was higher after irradiation with the heavy ions of high LET, and consequently the ratio of incomplete to complete exchanges increased steadily with LET up to 440 keV/microm, the highest LET included in the present study. For samples exposed to 200 MeV/nucleon iron ions, chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique, which allows identification of both complex and truly incomplete exchanges. Results of the mFISH study showed that 0.7 and 3 Gy iron ions produced similar ratios of complex to simple exchanges and incomplete to complete exchanges; these ratios were higher than those obtained after exposure to 6 Gy gamma rays. After 0.7 Gy of iron ions, most complex aberrations were found to involve three or four chromosomes, which is a likely indication of the maximum number of chromosome domains traversed by a single iron-ion track.

NASA Discipline Radiation Health↗
NASA NTRS2003

Biological Effectiveness of Accelerated Particles for the Induction of Chromosome Damage Measured in Metaphase and Interphase Human Lymphocytes

Chromosome aberrations were investigated in human lymphocytes after in vitro exposure to 1H-, 3He-, 12C-, 40Ar-, 28Si-, 56Fe-, or 197Au-ion beams, with LET ranging from approximately 0.4-1393 keV/microm in the dose range of 0.075-3 Gy. Dose-response curves for chromosome exchanges, measured at the first mitosis postirradiation using fluorescence in situ hybridization (FISH) with whole-chromosome probes, were fitted with linear or linear-quadratic functions. The relative biological effectiveness (RBE) was estimated from the initial slope of the dose-response curve for chromosomal damage with respect to low- or high-dose-rate gamma rays. Estimates of RBEmax values for mitotic spreads, which ranged from near 0.7 to 11.1 for total exchanges, increased with LET, reaching a maximum at about 150 keV/microm, and decreased with further increase in LET. RBEs for complex aberrations are undefined due to the lack of an initial slope for gamma rays. Additionally, the effect of mitotic delay on RBE values was investigated by measuring chromosome aberrations in interphase after chemically induced premature chromosome condensation (PCC), and values were up to threefold higher than for metaphase analysis.

NASA Discipline Radiation Health↗
NASA NTRS2002

Analysis of unrejoined chromosomal breakage in human fibroblast cells exposed to low- and high-LET radiation

Reported studies of DNA breakage induced by radiation of various qualities have generally shown a higher fraction of unrejoined residual breaks after high-LET exposure. This observation is supported by the argument that high-LET radiation induced DNA breaks that are more complex in nature and, thus, less likely to be repaired. In most cases the doses used in these studies were very high. We have studied unrejoined chromosome breaks by analyzing chromosome aberrations using a fluorescence in situ hybridization (FISH) technique with a combination of whole chromosome specific probes and probes specific for the telomere region of the chromosomes. Confluent human fibroblast cells (AG1522) were irradiated with gamma rays, 490 MeV/nucleon Si, or with Fe ions at either 200 and 500 MeV/nucleon, and were allowed to repair at 37 degrees C for 24 hours after exposure. A chemically induced premature chromosome condensation (PCC) technique was used to condense chromosomes in the G2 phase of the cell cycle. Results showed that the frequency of unrejoined chromosome breaks was higher after high-LET radiation, and the ratio of unrejoined to misrejoined chromosome breaks increased steadily with LET up a peak value at 440 keV/microm.

NASA Discipline Radiation Health↗
NASA NTRS2003

Radiation-induced chromosome aberrations in ataxia telangiectasia cells: high frequency of deletions and misrejoining detected by fluorescence in situ hybridization

The mechanisms underlying the hyper-radiosensitivity of AT cells were investigated by analyzing chromosome aberrations in the G(2) and M phases of the cell cycle using a combination of chemically induced premature chromosome condensation (PCC) and fluorescence in situ hybridization (FISH) with chromosome painting probes. Confluent cultures of normal fibroblast cells (AG1522) and fibroblast cells derived from an individual with AT (GM02052) were exposed to gamma rays and allowed to repair at 37 degrees C for 24 h. At doses that resulted in 10% survival, GM02052 cells were approximately five times more sensitive to gamma rays than AG1522 cells. For a given dose, GM02052 cells contained a much higher frequency of deletions and misrejoining than AG1522 cells. For both cell types, a good correlation was found between the percentage of aberrant cells and cell survival. The average number of color junctions, which represent the frequency of chromosome misrejoining, was also found to correlate well with survival. However, in a similar surviving population of GM02052 and AG1522 cells, induced by 1 Gy and 6 Gy, respectively, AG1522 cells contained four times more color junctions and half as many deletions as GM02052 cells. These results indicate that both repair deficiency and misrepair may be involved in the hyper-radiosensitivity of AT cells.

NASA Center JSC↗
NASA NTRS2003

Pigment composition and concentrations within the plant (Ceratophyllum demersum L.) component of the STS-89 C.E.B.A.S. Mini-Module spaceflight experiment

The Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) Mini-Module, a Space Shuttle middeck locker payload which supports a variety of aquatic inhabitants (fish, snails, plants and bacteria) in an enclosed 8.6 L chamber, was tested for its biological stability in microgravity. The aquatic plant, Ceratophyllum demersum L., was critical for the vitality and functioning of this artificial mini-ecosystem. Its photosynthetic pigment concentrations were of interest due to their light harvesting and protective functions. "Post-flight" chlorophyll and carotenoid concentrations within Ceratophyllum apical segments were directly related to the quantities of light received in the experiments, with microgravity exposure (STS-89) failing to account for any significant deviation from ground control studies. Published by Elsevier Science Ltd on behalf of COSPAR.

short duration↗
NASA NTRS2002

Chromosome aberrations in human lymphocytes induced by 250 MeV protons: effects of dose, dose rate and shielding

Although the space radiation environment consists predominantly of energetic protons, astronauts inside a spacecraft are chronically exposed to both primary particles as well as secondary particles that are generated when the primary particles penetrate the spacecraft shielding. Secondary neutrons and secondary charged particles can have an LET value that is greater than the primary protons and, therefore, produce a higher relative biological effectiveness (RBE). Using the accelerator facility at Loma Linda University, we exposed human lymphocytes in vitro to 250 MeV protons with doses ranging from 0 to 60 cGy at three different dose rates: a low dose rate of 7.5 cGy/h, an intermediate dose rate of 30 cGy/h and a high dose rate of 70 cGy/min. The effect of 15 g/cm2 aluminum shielding on the induction of chromosome aberrations was investigated for each dose rate. After exposure, lymphocytes were incubated in growth medium containing phytohemagglutinin (PHA) and chromosome spreads were collected using a chemical-induced premature chromosome condensation (PCC) technique. Aberrations were analyzed using the fluorescence in situ hybridization (FISH) technique with three different colored chromosome-painting probes. The frequency of reciprocal and complex-type chromosome exchanges were compared in shielded and unshielded samples. c2002 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Non-NASA Center↗
NASA NTRS2002

Sexually dimorphic effects of NMDA receptor antagonism on brain-pituitary-gonad axis development in the platyfish

The N-methyl-D-aspartate glutamate receptor (NMDAR) is found in hypothalamic nuclei involved in the regulation of reproduction in several species of mammals and fishes. NMDAR is believed to affect reproductive development and function by regulating gonadotropin releasing hormone (GnRH)-producing cells. These pathways are likely to be sexually dimorphic, as are several other neurotransmitter systems involved in reproductive function. In this report, male and female platyfish received intraperitoneal injections of 0, 5, 10, 20, 40 or 60 microg/g body wt. of the non-competitive NMDAR antagonist MK-801. Injections began at 6 weeks of age and continued thrice weekly until control animals reached puberty, as evidenced by anal fin maturation. The percent of pubescent animals was significantly affected by sex and treatment, with fewer MK-801-injected females in puberty than control females at each dose (P<0.001), and fewer pubescent females than males at 10, 20 and 40 microg/g (P<0.05). There were no MK-801-related effects in males. Histological analyses revealed typical immature gonads and pituitary glands in treated females, and typical mature morphology in control females and all males. Immunocytochemical distribution of the R1 subunit of the NMDAR within the brain-pituitary-gonad (BPG) axis was limited to GnRH-containing brain cells in all animals; however, NMDAR1 distribution was in an immature pattern in treated females and a mature pattern in all others. Neural concentrations of GnRH were unaffected by MK-801 treatment in both sexes. These data suggest that in the platyfish, NMDAR influence on reproductive development is sexually dimorphic and occurs at, or above, the level of GnRH-containing cells of the BPG axis.

Non-NASA Center↗
NASA NTRS2001

Comparison of chromosome aberration frequencies in pre- and post-flight astronaut lymphocytes irradiated in vitro with gamma rays

If radiosensitivity is altered in a microgravity environment, it will affect the accuracy of assessing astronauts' risk from exposure to space radiation. To investigate the effects of space flight on radiosensitivity, we exposed a crewmember's blood to gamma rays at doses ranging from 0 to 3 Gy and analyzed chromosome aberrations in mitotic lymphocytes. The blood samples were collected 10 days prior to an 8-day Shuttle mission, the day the flight returned, and 14 days after the flight. After exposure, lymphocytes were stimulated to grow in media containing phytohaemagglutinin (PHA) and mitotic cells were harvested for chromosome analysis using a fluorescence in situ hybridization (FISH) with whole chromosome specific probes. The dose response of total exchanges showed no changes in the radiosensitivity after the mission.

manned↗
NASA NTRS2001

Chromosome aberrations in the blood lymphocytes of astronauts after space flight

Cytogenetic analysis of the lymphocytes of astronauts provides a direct measurement of space radiation damage in vivo, which takes into account individual radiosensitivity and considers the influence of microgravity and other stress conditions. Chromosome exchanges were measured in the blood lymphocytes of eight crew members after their respective space missions, using fluorescence in situ hybridization (FISH) with chromosome painting probes. Significant increases in aberrations were observed after the long-duration missions. The in vivo dose was derived from the frequencies of translocations and total exchanges using calibration curves determined before flight, and the RBE was estimated by comparison with individually measured physical absorbed doses. The values for average RBE were compared to the average quality factor (Q) from direct measurements of the lineal energy spectra using a tissue-equivalent proportional counter (TEPC) and radiation transport codes. The ratio of aberrations identified as complex was slightly higher after flight, which is thought to be an indication of exposure to high-LET radiation. To determine whether the frequency of complex aberrations measured in metaphase spreads after exposure to high-LET radiation was influenced by a cell cycle delay, chromosome damage was analyzed in prematurely condensed chromosome samples collected from two crew members before and after a short-duration mission. The frequency of complex exchanges after flight was higher in prematurely condensed chromosomes than in metaphase cells for one crew member.

NASA Center JSC↗
NASA NTRS1996

Radiogenic transformation of human mammary epithelial cells in vitro

Cancer induction by space radiations is a major concern for manned space exploration. Accurate assessment of radiation risk at low doses requires basic understanding of mechanism(s) of radiation carcinogenesis. For determining the oncogenic effects of ionizing radiation in human epithelial cells, we transformed a mammary epithelial cell line (185B5), which was immortalized by benzo(a)pyrene, with energetic heavy ions and obtained several transformed clones. These transformed cells showed growth properties on Matrigel similar to human mammary tumor cells. To better understand the mechanisms of radiogenic transformation of human cells, we systematically examined the alterations in chromosomes and cancer genes. Among 16 autosomes examined for translocations, by using fluorescence in situ hybridization (FISH) technique, chromosomes 3, 12, 13, 15, 16, and 18 appeared to be normal in transformed cells. Chromosomes 1, 4, 6, 8, and 17 in transformed cells, however, showed patterns different from those in nontransformed cells. Southern blot analyses indicated no detectable alterations in myc, ras, Rb, or p53 genes. Further studies of chromosome 17 by using in situ hybridization with unique sequence p53 gene probe and a centromere probe showed no loss of p53 gene in transformed cells. Experimental results from cell fusion studies indicated that the transforming gene(s) is recessive. The role of genomic instability and tumor suppressor gene(s) in radiogenic transformation of human breast cells remains to be identified.

NASA Center JSC↗